RchiOBHm_Chr2g0157561

Ribonuclease z

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0015269
Physical Location & Seq
Forward (+)
73897235 .. 73900242
3008 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52629

Sequence Viewer

Length: 1062 bp
ATGCAAATCTCTCTGACCATTACACCCTCCAAAGCCCCTCTAATTTTCCCACTCCACCACCCCATTTCCCAAACCCCCCCAAAACCCCACACAACTTCCCTTCAAACCCTCTCCAATCCTCTCAATTCCCTCAAATCCTCCTCGCCTTATCTATCTGCAGTGGGCCGGGCTATCGAGGACGACGAGTACCGGATAGCCCGGAACCAGGTTCTCCGAAAAGGTGTGGACTTGGAGGGCTACTCCATTGAGGGGGTCTCCGTCGGGGGTCATGAGACTTGTATCATTATACCGGAGTTTAAGTGTGCTTTCGATATCGGCAGGTGTCCCTCCAGAGCCATTCATCAGAATTTTCTCTTCATCACTCATGCCCATCTTGACCACATTGGTGGGCTGCCGATGTATGTAGCCAGCCGTGGTTTGTACAACTTAAAGCCTCCGACTGTGTTCGTGCCTCCTTGTATTAAAGAGGATGTGGAGAAGCTGATGGACATTCACAGGAGCATGGGACATGTGGAGCTGGAACTTGAATTGGTTGCATTGGATGTGGGTGAAACATATGAGCTGCGGAATAACCTTGTTGTCCGGCCATTTAGGACTCAGCATGCCATACCCAGCCAGGGTTATGTTATTTACTCGGTTAGAAAAAAACTGAAGAAGCAGTACATGCATCTGAAAGGGAAACAGATTGAGAAATTGAAAAAGTCTGGTACTGAGATTACAGACACTATATTGTCACCAGAGGTGGCCTTCACAGGGGATACAACATCGGAGTATATGCTGGATCCACGTAATGCTGATGCCTTGAGGGCAAAGATTCTCATAACTGAGGCAACTTTTCTGGATGAAGGATTCAGTATTGAGGATGCACGACAACATGGTCATACGCATATAAATGAGATCATTGAAAATGCTCAGTGGATTAGAAATAAGGCAATTTTGTTGACCCATTTCTCCTCCCGCTACCATTTAGAGGACATTCGTCAAGCTGTATCAAGGTTGCAGTCTAAGGTGTCAGCGAAAGTGGTCCCTCTTACAGAAGGTTTCAAATCCATGCACACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Lactamase_B_2

Protein Analysis

353

Amino Acids

39.76

Weight (kDa)

8.24

Isoelectric Point (pI)

47.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B_2 PF12706 116 - 317 8e-11 Beta-lactamase superfamily domain
Anti-Pycsar_Apyc1 PF23023 117 - 321 1.4e-07 Anti-Pycsar protein Apyc1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04530
fragaria_vesca FvH4_6g41771 FvH4_6g41771 FvH4_6g41771 FvH4_6g41771
malus_domestica MD09G1117300.v1.1
prunus_persica Prupe.3G206500_v2.0.a1
pyrus_communis pycom09g04240
rosa_chinensis RchiOBHm_Chr2g0157561
rosa_laevigata RLG00000020998
rosa_roxburghii Rroxscaffold_2G00092120
rosa_rugosa Rorug02G0462100
rosa_samantha Rh2AG526200 Rh2BG540900 Rh2CG510900 Rh2DG549400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 309
AasI GACNNNNNNGTC 1 cut(s) 876
Acc36I ACCTGC 1 cut(s) 309
AciI CCGC 2 cut(s) 565, 958
AclWI GGATC 2 cut(s) 776, 789
AcoI YGGCCR 1 cut(s) 584
AcsI RAATTY 1 cut(s) 346
AcuI CTGAAG 1 cut(s) 671
AfaI GTAC 4 cut(s) 188, 422, 662, 709
AfiI CCNNNNNNNGG 5 cut(s) 205, 249, 617, 753, 970
AflIII ACRYGT 1 cut(s) 508
AgsI TTSAA 5 cut(s) 104, 527, 697, 905, 1045
AjnI CCWGG 2 cut(s) 204, 615
AleI CACNNNNGTG 1 cut(s) 384
AluBI AGCT 4 cut(s) 481, 517, 562, 986
AluI AGCT 4 cut(s) 481, 517, 562, 986
Alw26I GTCTC 2 cut(s) 259, 266
AlwI GGATC 2 cut(s) 776, 789
AoxI GGCC 3 cut(s) 163, 584, 744
ApeKI GCWGC 2 cut(s) 391, 562
ApoI RAATTY 1 cut(s) 346
AspS9I GGNCC 2 cut(s) 163, 1024
AsuC2I CCSGG 2 cut(s) 167, 199
AsuHPI GGTGA 2 cut(s) 560, 726
AvaII GGWCC 1 cut(s) 1024
BamHI GGATCC 1 cut(s) 781
BarI GAAGNNNNNNTAC 2 cut(s) 644, 676
BbvI GCAGC 2 cut(s) 378, 549
BccI CCATC 2 cut(s) 378, 478
BceAI ACGGC 1 cut(s) 396
BciT130I CCWGG 2 cut(s) 206, 617
BciVI GTATCC 1 cut(s) 751
BcnI CCSGG 2 cut(s) 167, 199
BcoDI GTCTC 2 cut(s) 259, 266
BfmI CTRYAG 1 cut(s) 156
BfuAI ACCTGC 1 cut(s) 309
BfuI GTATCC 1 cut(s) 751
BglI GCCNNNNNGGC 1 cut(s) 806
BisI GCNGC 2 cut(s) 392, 563
BlsI GCNGC 2 cut(s) 393, 564
Bme1390I CCNGG 4 cut(s) 167, 199, 206, 617
Bme18I GGWCC 1 cut(s) 1024
BmgT120I GGNCC 2 cut(s) 163, 1024
BmiI GGNNCC 3 cut(s) 203, 783, 1026
BmrFI CCNGG 4 cut(s) 167, 199, 206, 617
BmsI GCATC 3 cut(s) 676, 787, 853
BoxI GACNNNNGTC 1 cut(s) 978
BplI GAGNNNNNCTC 4 cut(s) 224, 256, 239, 271
BpmI CTGGAG 1 cut(s) 313
BpuEI CTTGAG 1 cut(s) 823
BpuMI CCSGG 2 cut(s) 167, 199
BsaAI YACGTR 1 cut(s) 788
BsaI GGTCTC 1 cut(s) 259
BsaJI CCNNGG 2 cut(s) 412, 616
BsaWI WCCGGW 2 cut(s) 189, 289
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 5 cut(s) 205, 249, 617, 753, 970
BseBI CCWGG 2 cut(s) 206, 617
BseDI CCNNGG 2 cut(s) 412, 616
BseGI GGATG 4 cut(s) 475, 547, 847, 868
BseLI CCNNNNNNNGG 5 cut(s) 205, 249, 617, 753, 970
BseMII CTCAG 4 cut(s) 611, 702, 816, 926
BseRI GAGGAG 2 cut(s) 130, 943
BseXI GCAGC 2 cut(s) 378, 549
BseYI CCCAGC 1 cut(s) 611
BshFI GGCC 3 cut(s) 165, 586, 746
BsiSI CCGG 5 cut(s) 166, 190, 199, 290, 583
BslFI GGGAC 3 cut(s) 309, 519, 1010
BslI CCNNNNNNNGG 5 cut(s) 205, 249, 617, 753, 970
BsmAI GTCTC 2 cut(s) 259, 266
BsmFI GGGAC 3 cut(s) 309, 519, 1010
BsnI GGCC 3 cut(s) 165, 586, 746
Bso31I GGTCTC 1 cut(s) 259
Bsp1407I TGTACA 1 cut(s) 420
Bsp143I GATC 2 cut(s) 781, 897
BspACI CCGC 2 cut(s) 565, 958
BspANI GGCC 3 cut(s) 165, 586, 746
BspCNI CTCAG 4 cut(s) 610, 703, 817, 925
BspHI TCATGA 1 cut(s) 268
BspLI GGNNCC 3 cut(s) 203, 783, 1026
BspMAI CTGCAG 1 cut(s) 160
BspMI ACCTGC 1 cut(s) 309
BspPI GGATC 2 cut(s) 776, 789
BspTNI GGTCTC 1 cut(s) 259
BsrGI TGTACA 1 cut(s) 420
BssECI CCNNGG 2 cut(s) 412, 616
BssMI GATC 2 cut(s) 781, 897
Bst2UI CCWGG 2 cut(s) 206, 617
Bst4CI ACNGT 1 cut(s) 442
Bst6I CTCTTC 1 cut(s) 359
BstAPI GCANNNNNTGC 1 cut(s) 664
BstAUI TGTACA 1 cut(s) 420
BstBAI YACGTR 1 cut(s) 788
BstC8I GCNNGC 2 cut(s) 409, 603
BstDEI CTNAG 6 cut(s) 597, 711, 825, 912, 1005, 1059
BstDSI CCRYGG 1 cut(s) 412
BstF5I GGATG 4 cut(s) 475, 547, 847, 868
BstKTI GATC 2 cut(s) 784, 900
BstMAI GTCTC 2 cut(s) 259, 266
BstMBI GATC 2 cut(s) 781, 897
BstMWI GCNNNNNNNGC 2 cut(s) 664, 806
BstNI CCWGG 2 cut(s) 206, 617
BstNSI RCATGY 3 cut(s) 512, 605, 667
BstPAI GACNNNNGTC 1 cut(s) 978
BstSCI CCNGG 4 cut(s) 165, 197, 204, 615
BstSFI CTRYAG 1 cut(s) 156
BstV1I GCAGC 2 cut(s) 378, 549
BstX2I RGATCY 1 cut(s) 781
BstXI CCANNNNNNTGG 1 cut(s) 386
BstYI RGATCY 1 cut(s) 781
BsuI GTATCC 1 cut(s) 751
BsuRI GGCC 3 cut(s) 165, 586, 746
BtgI CCRYGG 1 cut(s) 412
BtsCI GGATG 4 cut(s) 475, 547, 847, 868
BtsI GCAGTG 1 cut(s) 165
BtsIMutI CAGTG 2 cut(s) 165, 920
BveI ACCTGC 1 cut(s) 309
Cac8I GCNNGC 2 cut(s) 409, 603
CciI TCATGA 1 cut(s) 268
Cfr13I GGNCC 2 cut(s) 163, 1024
CsiI ACCWGGT 1 cut(s) 204
Csp6I GTAC 4 cut(s) 187, 421, 661, 708
CviAII CATG 8 cut(s) 269, 365, 502, 509, 602, 664, 875, 1051
CviQI GTAC 4 cut(s) 187, 421, 661, 708
DdeI CTNAG 6 cut(s) 597, 711, 825, 912, 1005, 1059
DpnI GATC 2 cut(s) 783, 899
DpnII GATC 2 cut(s) 781, 897
DrdI GACNNNNNNGTC 1 cut(s) 876
DseDI GACNNNNNNGTC 1 cut(s) 876
EaeI YGGCCR 1 cut(s) 584
Eam1104I CTCTTC 1 cut(s) 359
EarI CTCTTC 1 cut(s) 359
Eco31I GGTCTC 1 cut(s) 259
Eco32I GATATC 1 cut(s) 313
Eco47I GGWCC 1 cut(s) 1024
Eco57I CTGAAG 1 cut(s) 671
EcoRII CCWGG 2 cut(s) 204, 615
EcoRV GATATC 1 cut(s) 313
EcoT22I ATGCAT 1 cut(s) 669
FaeI CATG 8 cut(s) 272, 368, 505, 512, 605, 667, 878, 1054
FaqI GGGAC 3 cut(s) 309, 519, 1010
FatI CATG 8 cut(s) 268, 364, 501, 508, 601, 663, 874, 1050
FauI CCCGC 1 cut(s) 965
FauNDI CATATG 1 cut(s) 556
Fnu4HI GCNGC 2 cut(s) 392, 563
FokI GGATG 4 cut(s) 482, 554, 854, 875
Fsp4HI GCNGC 2 cut(s) 392, 563
GluI GCNGC 2 cut(s) 392, 563
GsaI CCCAGC 1 cut(s) 615
GsuI CTGGAG 1 cut(s) 313
HaeIII GGCC 3 cut(s) 165, 586, 746
HapII CCGG 5 cut(s) 166, 190, 199, 290, 583
Hin1II CATG 8 cut(s) 272, 368, 505, 512, 605, 667, 878, 1054
HincII GTYRAC 1 cut(s) 942
HindII GTYRAC 1 cut(s) 942
HinfI GANTC 3 cut(s) 595, 814, 849
HpaII CCGG 5 cut(s) 166, 190, 199, 290, 583
HphI GGTGA 2 cut(s) 560, 726
Hpy166II GTNNAC 2 cut(s) 226, 942
Hpy188I TCNGA 6 cut(s) 15, 215, 345, 438, 672, 769
Hpy188III TCNNGA 4 cut(s) 269, 330, 374, 839
Hpy8I GTNNAC 2 cut(s) 226, 942
Hpy99I CGWCG 2 cut(s) 185, 263
HpyAV CCTTC 4 cut(s) 110, 757, 839, 1031
HpyCH4III ACNGT 1 cut(s) 442
HpyCH4IV ACGT 1 cut(s) 787
HpyCH4V TGCA 7 cut(s) 4, 158, 536, 667, 866, 1000, 1054
HpyF10VI GCNNNNNNNGC 2 cut(s) 664, 806
HpyF3I CTNAG 6 cut(s) 597, 711, 825, 912, 1005, 1059
HpySE526I ACGT 1 cut(s) 787
Hsp92II CATG 8 cut(s) 272, 368, 505, 512, 605, 667, 878, 1054
Kzo9I GATC 2 cut(s) 781, 897
LmnI GCTCC 2 cut(s) 498, 514
Lsp1109I GCAGC 2 cut(s) 378, 549
LweI GCATC 3 cut(s) 676, 787, 853
MabI ACCWGGT 1 cut(s) 204
MaeII ACGT 1 cut(s) 787
MaeIII GTNAC 1 cut(s) 732
MalI GATC 2 cut(s) 783, 899
MboI GATC 2 cut(s) 781, 897
MboII GAAGA 2 cut(s) 346, 664
MflI RGATCY 1 cut(s) 781
MluCI AATT 6 cut(s) 42, 124, 346, 527, 692, 933
MlyI GAGTC 1 cut(s) 589
MmeI TCCRAC 1 cut(s) 461
Mph1103I ATGCAT 1 cut(s) 669
MseI TTAA 3 cut(s) 297, 428, 462
MslI CAYNNNNRTG 2 cut(s) 384, 891
MspI CCGG 5 cut(s) 166, 190, 199, 290, 583
MspR9I CCNGG 4 cut(s) 167, 199, 206, 617
MvaI CCWGG 2 cut(s) 206, 617
MwoI GCNNNNNNNGC 2 cut(s) 664, 806
NciI CCSGG 2 cut(s) 167, 199
NdeI CATATG 1 cut(s) 556
NdeII GATC 2 cut(s) 781, 897
NlaIII CATG 8 cut(s) 272, 368, 505, 512, 605, 667, 878, 1054
NlaIV GGNNCC 3 cut(s) 203, 783, 1026
NmuCI GTSAC 1 cut(s) 732
NsiI ATGCAT 1 cut(s) 669
NspI RCATGY 3 cut(s) 512, 605, 667
OliI CACNNNNGTG 1 cut(s) 384
PaeI GCATGC 1 cut(s) 605
PagI TCATGA 1 cut(s) 268
PaqCI CACCTGC 1 cut(s) 309
PciI ACATGT 1 cut(s) 508
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PfeI GAWTC 2 cut(s) 814, 849
PkrI GCNGC 2 cut(s) 393, 564
PleI GAGTC 1 cut(s) 589
PpsI GAGTC 1 cut(s) 589
Ppu21I YACGTR 1 cut(s) 788
PscI ACATGT 1 cut(s) 508
PshAI GACNNNNGTC 1 cut(s) 978
Psp6I CCWGG 2 cut(s) 204, 615
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 2 cut(s) 204, 615
PspN4I GGNNCC 3 cut(s) 203, 783, 1026
PspPI GGNCC 2 cut(s) 163, 1024
PstI CTGCAG 1 cut(s) 160
PsuI RGATCY 1 cut(s) 781
RsaI GTAC 4 cut(s) 188, 422, 662, 709
RsaNI GTAC 4 cut(s) 187, 421, 661, 708
RseI CAYNNNNRTG 2 cut(s) 384, 891
SaqAI TTAA 3 cut(s) 297, 428, 462
SatI GCNGC 2 cut(s) 392, 563
Sau3AI GATC 2 cut(s) 781, 897
Sau96I GGNCC 2 cut(s) 163, 1024
SchI GAGTC 1 cut(s) 589
ScrFI CCNGG 4 cut(s) 167, 199, 206, 617
SexAI ACCWGGT 1 cut(s) 204
SfaNI GCATC 3 cut(s) 676, 787, 853
SfcI CTRYAG 1 cut(s) 156
SinI GGWCC 1 cut(s) 1024
SmiMI CAYNNNNRTG 2 cut(s) 384, 891
SmlI CTYRAG 1 cut(s) 802
SmoI CTYRAG 1 cut(s) 802
SphI GCATGC 1 cut(s) 605
Sse9I AATT 6 cut(s) 42, 124, 346, 527, 692, 933
SsiI CCGC 2 cut(s) 565, 958
StyD4I CCNGG 4 cut(s) 165, 197, 204, 615
TaaI ACNGT 1 cut(s) 442
TaiI ACGT 1 cut(s) 790
TaqI TCGA 2 cut(s) 174, 309
TasI AATT 6 cut(s) 42, 124, 346, 527, 692, 933
TatI WGTACW 2 cut(s) 420, 660
TfiI GAWTC 2 cut(s) 814, 849
Tru1I TTAA 3 cut(s) 297, 428, 462
Tru9I TTAA 3 cut(s) 297, 428, 462
TscAI CASTG 2 cut(s) 165, 920
TseFI GTSAC 1 cut(s) 732
TseI GCWGC 2 cut(s) 391, 562
Tsp45I GTSAC 1 cut(s) 732
TspDTI ATGAA 3 cut(s) 329, 346, 858
TspGWI ACGGA 1 cut(s) 247
TspRI CASTG 2 cut(s) 165, 920
VpaK11BI GGWCC 1 cut(s) 1024
XapI RAATTY 1 cut(s) 346
XceI RCATGY 3 cut(s) 512, 605, 667
Zsp2I ATGCAT 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.