FvH4_6g43210

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011585
Physical Location & Seq
Forward (+)
33486612 .. 33490770
4159 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g43210.t1

Sequence Viewer

Length: 1329 bp
ATGAGGCAAAGTGTCTCTTATAGTCTAAACCTGCTCACTCTTAGTCTGCTTTTCAGTCAATGTGTATATGAGTGCCATTGTGGGTTAGTTGGTGATGCGGAAGCAATGGCTCCTGATATCTCTTCCAGTGTGAACCCTCAACCTTTTCTGCCTTTATTAGCGCCTTCACCATTGACACCATTCACAAATGATAGCATTCCAACATTATCAGGTCTCTGTACATTGAATTTTTCAGTTGTCGGTGATCTGATGAGCAAAACAGCCACCGACTGCTGGGCCTTTTTTGCGCCTGTATTGGCTAATGTAGTGTGTTGTCCTCAGCTTGAGGCCACCTTGACGACTCTTATAGGGCACTCCAGTAAATACTCTAGGATGCTGTCTTTGGATACAACTCATGCAAAACACTGCCTCTCAGATGTTGAGAAGATCCTAGAGAGTCAAGGAGCCAATAAGACTATTCAAAAGATATGCTCAATTCAACCAGAAAACCTCACTGAAGCCTCTTGCCCTGTTATGGAGGTTGATACGTTTGAGAGCATTGTGGATTCTTCGAAACTACTTGCTGCTTGCCAAAAAGTTGATCCTGTCAATGAGTGTTGTCAGGAAGTTTGCCAAAATGCCATATTAGATGCTTCTAGAAAACTTGCTGATATTGGTATTGCTGCAAATTTGGAAGGTGTCCATGCTGTACCTCAACAGTCATCCAGGATAGACGATTGTAAGAATATTGTGCTGCGATGGCTAGCGAGTAACCTTGATCCTTCTTCTGCAAATAAGGTCCTTAGAGGACTATCAAATTGCAAAGTAAATAAAGTGTGTCCTCTTGTTTTCCCCAACATGACACGTGTTGTGAAAGAATGTGCAAATATGATAACAAGCCAGGCCTCCTGTTGTAAAGCAATGGATAGTTATGTTTCCCAGTTGCAAGAGCAGAGCTTTATTACAAACTTGCAGGCCTTAAATTGTGCTGCGTCACTTGGAGTGAAGTTGCAGAAAGCTAATGTATCCAATAATGTTTATCAACTTTGTCACATAAACCTCAAGGACTTTTCTCTTCAAGTTGGATCACAAGAGTATGGCTGCCTTCTGCCAAGCCTGCCTACAGATGCAACATTTGACAAAACTTCGGGAGTCAGCTTCATTTGTGATCTAAATGACAACATTGCAGCTCCGTGGCCCTCTTCATTTTCTGAACCTCCATCGACATGCAACAGAACTACCAAAATCCCAGCAGTACCCAAAGCAACATCTGCACAAAGCGGCCTGTACAAGAATTTGATGCTTCCTTCTTTCTTTTCCTCCTTGGTGGTTCTCAAGCTGCTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

443

Amino Acids

47.7

Weight (kDa)

5.57

Isoelectric Point (pI)

54.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPARK PF19160 70 - 218 6.3e-23 SPARK
At1g61900 PF26584 271 - 344 9.6e-35 At1g61900-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 39
AciI CCGC 2 cut(s) 98, 1260
AclWI GGATC 4 cut(s) 421, 575, 752, 1072
AcsI RAATTY 3 cut(s) 226, 667, 1273
AcuI CTGAAG 1 cut(s) 516
AcvI CACGTG 1 cut(s) 845
AfaI GTAC 4 cut(s) 220, 690, 1236, 1268
AfiI CCNNNNNNNGG 2 cut(s) 273, 514
AflIII ACRYGT 2 cut(s) 842, 844
AgsI TTSAA 4 cut(s) 226, 461, 479, 1058
AjnI CCWGG 2 cut(s) 704, 879
AluBI AGCT 6 cut(s) 322, 936, 998, 1137, 1169, 1318
AluI AGCT 6 cut(s) 322, 936, 998, 1137, 1169, 1318
Alw26I GTCTC 2 cut(s) 19, 218
AlwI GGATC 4 cut(s) 421, 575, 752, 1072
AoxI GGCC 6 cut(s) 276, 327, 882, 954, 1175, 1261
ApeKI GCWGC 7 cut(s) 563, 662, 733, 968, 1080, 1166, 1318
ApoI RAATTY 3 cut(s) 226, 667, 1273
AspLEI GCGC 2 cut(s) 163, 289
AspS9I GGNCC 3 cut(s) 276, 778, 1176
AsuHPI GGTGA 3 cut(s) 104, 159, 254
AsuII TTCGAA 1 cut(s) 551
AsuNHI GCTAGC 1 cut(s) 742
AvaII GGWCC 1 cut(s) 778
BaeGI GKGCMC 1 cut(s) 354
BbrPI CACGTG 1 cut(s) 845
BbvCI CCTCAGC 1 cut(s) 318
BbvI GCAGC 7 cut(s) 550, 649, 720, 955, 1067, 1178, 1305
BccI CCATC 2 cut(s) 732, 1207
BciT130I CCWGG 2 cut(s) 706, 881
BciVI GTATCC 2 cut(s) 379, 1015
BcoDI GTCTC 2 cut(s) 19, 218
BfaI CTAG 4 cut(s) 369, 431, 636, 743
BfmI CTRYAG 1 cut(s) 1101
BfoI RGCGCY 1 cut(s) 164
BfuAI ACCTGC 1 cut(s) 39
BfuI GTATCC 2 cut(s) 379, 1015
BisI GCNGC 8 cut(s) 564, 663, 734, 969, 1081, 1167, 1261, 1319
BlsI GCNGC 8 cut(s) 565, 664, 735, 970, 1082, 1168, 1262, 1320
Bme1390I CCNGG 2 cut(s) 706, 881
Bme18I GGWCC 1 cut(s) 778
BmgT120I GGNCC 3 cut(s) 276, 778, 1176
BmiI GGNNCC 2 cut(s) 111, 445
BmrFI CCNGG 2 cut(s) 706, 881
BmrI ACTGGG 1 cut(s) 913
BmsI GCATC 5 cut(s) 85, 363, 619, 1096, 1269
BmtI GCTAGC 1 cut(s) 746
BmuI ACTGGG 1 cut(s) 913
BpmI CTGGAG 1 cut(s) 340
Bpu10I CCTNAGC 1 cut(s) 318
Bpu14I TTCGAA 1 cut(s) 551
BpuEI CTTGAG 3 cut(s) 344, 1025, 1298
BsaAI YACGTR 1 cut(s) 845
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 2 cut(s) 1172, 1302
Bsc4I CCNNNNNNNGG 2 cut(s) 273, 514
Bse1I ACTGG 3 cut(s) 126, 357, 919
Bse3DI GCAATG 3 cut(s) 111, 906, 1161
BseBI CCWGG 2 cut(s) 706, 881
BseDI CCNNGG 2 cut(s) 1172, 1302
BseGI GGATG 2 cut(s) 378, 701
BseLI CCNNNNNNNGG 2 cut(s) 273, 514
BseMI GCAATG 3 cut(s) 111, 906, 1161
BseMII CTCAG 2 cut(s) 332, 426
BseNI ACTGG 3 cut(s) 126, 357, 919
BseSI GKGCMC 1 cut(s) 354
BseXI GCAGC 7 cut(s) 550, 649, 720, 955, 1067, 1178, 1305
BseYI CCCAGC 2 cut(s) 273, 1228
BsgI GTGCAG 1 cut(s) 1236
BshFI GGCC 6 cut(s) 278, 329, 884, 956, 1177, 1263
BslI CCNNNNNNNGG 2 cut(s) 273, 514
BsmAI GTCTC 2 cut(s) 19, 218
BsmI GAATGC 1 cut(s) 195
BsnI GGCC 6 cut(s) 278, 329, 884, 956, 1177, 1263
Bso31I GGTCTC 1 cut(s) 218
Bsp119I TTCGAA 1 cut(s) 551
Bsp1286I GDGCHC 1 cut(s) 354
Bsp1407I TGTACA 2 cut(s) 218, 1266
Bsp143I GATC 6 cut(s) 244, 426, 580, 757, 1064, 1147
BspACI CCGC 2 cut(s) 98, 1260
BspANI GGCC 6 cut(s) 278, 329, 884, 956, 1177, 1263
BspCNI CTCAG 2 cut(s) 331, 425
BspLI GGNNCC 2 cut(s) 111, 445
BspMI ACCTGC 1 cut(s) 39
BspOI GCTAGC 1 cut(s) 746
BspPI GGATC 4 cut(s) 421, 575, 752, 1072
BspT104I TTCGAA 1 cut(s) 551
BspTNI GGTCTC 1 cut(s) 218
BsrDI GCAATG 3 cut(s) 111, 906, 1161
BsrGI TGTACA 2 cut(s) 218, 1266
BsrI ACTGG 3 cut(s) 126, 357, 919
BssECI CCNNGG 2 cut(s) 1172, 1302
BssMI GATC 6 cut(s) 244, 426, 580, 757, 1064, 1147
BssT1I CCWWGG 1 cut(s) 1302
Bst2UI CCWGG 2 cut(s) 706, 881
Bst4CI ACNGT 1 cut(s) 699
Bst6I CTCTTC 3 cut(s) 127, 1059, 1186
BstAPI GCANNNNNTGC 1 cut(s) 1250
BstAUI TGTACA 2 cut(s) 218, 1266
BstBAI YACGTR 1 cut(s) 845
BstBI TTCGAA 1 cut(s) 551
BstC8I GCNNGC 4 cut(s) 568, 744, 954, 1097
BstDEI CTNAG 4 cut(s) 41, 318, 412, 782
BstDSI CCRYGG 1 cut(s) 1172
BstF5I GGATG 2 cut(s) 378, 701
BstH2I RGCGCY 1 cut(s) 164
BstHHI GCGC 2 cut(s) 163, 289
BstKTI GATC 6 cut(s) 247, 429, 583, 760, 1067, 1150
BstMAI GTCTC 2 cut(s) 19, 218
BstMBI GATC 6 cut(s) 244, 426, 580, 757, 1064, 1147
BstMWI GCNNNNNNNGC 4 cut(s) 284, 739, 1096, 1250
BstNI CCWGG 2 cut(s) 706, 881
BstNSI RCATGY 1 cut(s) 1209
BstSCI CCNGG 2 cut(s) 704, 879
BstSFI CTRYAG 1 cut(s) 1101
BstSLI GKGCMC 1 cut(s) 354
BstV1I GCAGC 7 cut(s) 550, 649, 720, 955, 1067, 1178, 1305
BstX2I RGATCY 1 cut(s) 426
BstYI RGATCY 1 cut(s) 426
BsuI GTATCC 2 cut(s) 379, 1015
BsuRI GGCC 6 cut(s) 278, 329, 884, 956, 1177, 1263
BtgI CCRYGG 1 cut(s) 1172
BtgZI GCGATG 1 cut(s) 751
BtsCI GGATG 2 cut(s) 378, 701
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 3 cut(s) 133, 403, 492
BveI ACCTGC 1 cut(s) 39
Cac8I GCNNGC 4 cut(s) 568, 744, 954, 1097
CfoI GCGC 2 cut(s) 163, 289
Cfr13I GGNCC 3 cut(s) 276, 778, 1176
CseI GACGC 1 cut(s) 960
Csp6I GTAC 4 cut(s) 219, 689, 1235, 1267
CviAII CATG 4 cut(s) 395, 683, 838, 1206
CviQI GTAC 4 cut(s) 219, 689, 1235, 1267
DdeI CTNAG 4 cut(s) 41, 318, 412, 782
DpnI GATC 6 cut(s) 246, 428, 582, 759, 1066, 1149
DpnII GATC 6 cut(s) 244, 426, 580, 757, 1064, 1147
Eam1104I CTCTTC 3 cut(s) 127, 1059, 1186
EarI CTCTTC 3 cut(s) 127, 1059, 1186
Eco130I CCWWGG 1 cut(s) 1302
Eco147I AGGCCT 2 cut(s) 884, 956
Eco31I GGTCTC 1 cut(s) 218
Eco32I GATATC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 778
Eco57I CTGAAG 1 cut(s) 516
Eco72I CACGTG 1 cut(s) 845
EcoO109I RGGNCCY 1 cut(s) 778
EcoRII CCWGG 2 cut(s) 704, 879
EcoRV GATATC 1 cut(s) 118
EcoT14I CCWWGG 1 cut(s) 1302
ErhI CCWWGG 1 cut(s) 1302
FaeI CATG 4 cut(s) 398, 686, 841, 1209
FalI AAGNNNNNCTT 1 cut(s) 33
FatI CATG 4 cut(s) 394, 682, 837, 1205
Fnu4HI GCNGC 8 cut(s) 564, 663, 734, 969, 1081, 1167, 1261, 1319
FokI GGATG 2 cut(s) 385, 688
Fsp4HI GCNGC 8 cut(s) 564, 663, 734, 969, 1081, 1167, 1261, 1319
FspBI CTAG 4 cut(s) 369, 431, 636, 743
GlaI GCGC 2 cut(s) 162, 288
GluI GCNGC 8 cut(s) 564, 663, 734, 969, 1081, 1167, 1261, 1319
GsaI CCCAGC 2 cut(s) 277, 1232
GsuI CTGGAG 1 cut(s) 340
HaeII RGCGCY 1 cut(s) 164
HaeIII GGCC 6 cut(s) 278, 329, 884, 956, 1177, 1263
HgaI GACGC 1 cut(s) 960
HhaI GCGC 2 cut(s) 163, 289
Hin1II CATG 4 cut(s) 398, 686, 841, 1209
Hin6I GCGC 2 cut(s) 161, 287
HinP1I GCGC 2 cut(s) 161, 287
HinfI GANTC 4 cut(s) 340, 436, 545, 1131
HphI GGTGA 3 cut(s) 104, 159, 254
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 4 cut(s) 249, 415, 1192, 1328
Hpy188III TCNNGA 4 cut(s) 113, 602, 636, 1128
Hpy8I GTNNAC 1 cut(s) 133
HpyAV CCTTC 5 cut(s) 174, 668, 771, 1094, 1296
HpyCH4III ACNGT 1 cut(s) 699
HpyCH4IV ACGT 2 cut(s) 527, 844
HpyF10VI GCNNNNNNNGC 4 cut(s) 284, 739, 1096, 1250
HpyF3I CTNAG 4 cut(s) 41, 318, 412, 782
HpySE526I ACGT 2 cut(s) 527, 844
Hsp92II CATG 4 cut(s) 398, 686, 841, 1209
HspAI GCGC 2 cut(s) 161, 287
Kzo9I GATC 6 cut(s) 244, 426, 580, 757, 1064, 1147
LmnI GCTCC 3 cut(s) 115, 443, 1174
Lsp1109I GCAGC 7 cut(s) 550, 649, 720, 955, 1067, 1178, 1305
LweI GCATC 5 cut(s) 85, 363, 619, 1096, 1269
MaeI CTAG 4 cut(s) 369, 431, 636, 743
MaeII ACGT 2 cut(s) 527, 844
MaeIII GTNAC 3 cut(s) 749, 972, 1028
MalI GATC 6 cut(s) 246, 428, 582, 759, 1066, 1149
MboI GATC 6 cut(s) 244, 426, 580, 757, 1064, 1147
MboII GAAGA 6 cut(s) 114, 436, 540, 756, 1046, 1173
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 1 cut(s) 354
MluCI AATT 6 cut(s) 226, 474, 667, 796, 961, 1273
MlyI GAGTC 3 cut(s) 334, 445, 1140
MmeI TCCRAC 2 cut(s) 224, 1042
MseI TTAA 1 cut(s) 959
MslI CAYNNNNRTG 1 cut(s) 1204
MspR9I CCNGG 2 cut(s) 706, 881
Mva1269I GAATGC 1 cut(s) 195
MvaI CCWGG 2 cut(s) 706, 881
MwoI GCNNNNNNNGC 4 cut(s) 284, 739, 1096, 1250
NdeII GATC 6 cut(s) 244, 426, 580, 757, 1064, 1147
NheI GCTAGC 1 cut(s) 742
NlaIII CATG 4 cut(s) 398, 686, 841, 1209
NlaIV GGNNCC 2 cut(s) 111, 445
NmuCI GTSAC 2 cut(s) 972, 1028
NspI RCATGY 1 cut(s) 1209
NspV TTCGAA 1 cut(s) 551
PceI AGGCCT 2 cut(s) 884, 956
PctI GAATGC 1 cut(s) 195
PfeI GAWTC 1 cut(s) 545
PfoI TCCNGGA 1 cut(s) 704
PkrI GCNGC 8 cut(s) 565, 664, 735, 970, 1082, 1168, 1262, 1320
PleI GAGTC 3 cut(s) 334, 444, 1139
PmaCI CACGTG 1 cut(s) 845
PmlI CACGTG 1 cut(s) 845
PpsI GAGTC 3 cut(s) 334, 444, 1139
Ppu21I YACGTR 1 cut(s) 845
PpuMI RGGWCCY 1 cut(s) 778
Psp5II RGGWCCY 1 cut(s) 778
Psp6I CCWGG 2 cut(s) 704, 879
PspCI CACGTG 1 cut(s) 845
PspFI CCCAGC 2 cut(s) 273, 1228
PspGI CCWGG 2 cut(s) 704, 879
PspN4I GGNNCC 2 cut(s) 111, 445
PspPI GGNCC 3 cut(s) 276, 778, 1176
PspPPI RGGWCCY 1 cut(s) 778
PsuI RGATCY 1 cut(s) 426
RsaI GTAC 4 cut(s) 220, 690, 1236, 1268
RsaNI GTAC 4 cut(s) 219, 689, 1235, 1267
RseI CAYNNNNRTG 1 cut(s) 1204
SaqAI TTAA 1 cut(s) 959
SatI GCNGC 8 cut(s) 564, 663, 734, 969, 1081, 1167, 1261, 1319
Sau3AI GATC 6 cut(s) 244, 426, 580, 757, 1064, 1147
Sau96I GGNCC 3 cut(s) 276, 778, 1176
SchI GAGTC 3 cut(s) 334, 445, 1140
ScrFI CCNGG 2 cut(s) 706, 881
SduI GDGCHC 1 cut(s) 354
SfaNI GCATC 5 cut(s) 85, 363, 619, 1096, 1269
SfcI CTRYAG 1 cut(s) 1101
SfuI TTCGAA 1 cut(s) 551
SinI GGWCC 1 cut(s) 778
SmiMI CAYNNNNRTG 1 cut(s) 1204
SmlI CTYRAG 3 cut(s) 323, 1040, 1313
SmoI CTYRAG 3 cut(s) 323, 1040, 1313
Sse9I AATT 6 cut(s) 226, 474, 667, 796, 961, 1273
SseBI AGGCCT 2 cut(s) 884, 956
SsiI CCGC 2 cut(s) 98, 1260
SspI AATATT 1 cut(s) 727
SspMI CTAG 4 cut(s) 369, 431, 636, 743
StuI AGGCCT 2 cut(s) 884, 956
StyD4I CCNGG 2 cut(s) 704, 879
StyI CCWWGG 1 cut(s) 1302
TaaI ACNGT 1 cut(s) 699
TaiI ACGT 2 cut(s) 530, 847
TaqI TCGA 2 cut(s) 551, 1202
TasI AATT 6 cut(s) 226, 474, 667, 796, 961, 1273
TatI WGTACW 2 cut(s) 218, 1266
TauI GCSGC 1 cut(s) 1263
TfiI GAWTC 1 cut(s) 545
Tru1I TTAA 1 cut(s) 959
Tru9I TTAA 1 cut(s) 959
TscAI CASTG 3 cut(s) 133, 410, 499
TseFI GTSAC 2 cut(s) 972, 1028
TseI GCWGC 7 cut(s) 563, 662, 733, 968, 1080, 1166, 1318
Tsp45I GTSAC 2 cut(s) 972, 1028
TspDTI ATGAA 2 cut(s) 1129, 1173
TspGWI ACGGA 1 cut(s) 1161
TspRI CASTG 3 cut(s) 133, 410, 499
VpaK11BI GGWCC 1 cut(s) 778
XapI RAATTY 3 cut(s) 226, 667, 1273
XbaI TCTAGA 1 cut(s) 635
XceI RCATGY 1 cut(s) 1209
XspI CTAG 4 cut(s) 369, 431, 636, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.