Rh2CG525800

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0011585
Physical Location & Seq
Forward (+)
70080847 .. 70085676
4830 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG525800.1

Sequence Viewer

Length: 1296 bp
ATGAGGCAGAGGGTCTCTTATGGTCCAGGGCTCCTTACTCTTGTTCTACTTTTCAGTCTCTGTGTATCTGAGTCCCAATGTGGGTCAGTTGGCGATGTGGAGGCAATTGCTCCTGATATCTCATCCAGTGTGAACCCTCAACCTTTTCTGCCTTTATTAGCACCTTCACCATTGACACCATTGACACCATTCACAAATGACAGCATTCCGACATTATCAGGACTCTGTACATTGAATTTTACAGCTGCCAGTGATATGATGAGCAAAACAGCAACCGACTGCTGGGCCTTTTTTGCGCCCGTATTGGCTAATGTAGTGTGTTGCCCTCAGCTTGAGGCCACCTTGACCACTCTTATAGGGCACTCCAGTAAATACTCTAGGATGCTCTCTTTGAATATAACTCATGCAAAGCACTGCCTCTCAGATGTTGAGAAGATCCTAGAGAGTCAAGAAGCCAATAAGACTGTTCAAAAGATTTGCTCAATTCAACCAAAAAACCTCACTGAAGCTTCTTGCCCTGTTACAAAGGTTGATGCGTTTGAGAGCATTGTGGATTCTTCAAAACTACTTGCTGCTTGCCAAAAAGTTGATCCTGTCAATGAGTGTTGTCAGGAAGTTTGCCAAAATGCCATATTAGATGCTGCTAGAAAACTTGCTGAGATTGGTATTGCTGCAAATTTGGAAGGGGTCCATTCCGTACCTGAACAGTCAACCAGGATAGATGATTGTAAGAATATTGTGCTGCGATGGCTAGCGAGTAACCTTGATCCTTCTTCTGCAAATAAGGTCCTTAGAGGACTTTCAAATTGCAAAGTAAATAAAGTGTGTCCTCTTATTTTCCCCAACATGACACGTGTTGTGAAAGAATGTGCAAATATGATAACAAGCCAGGCTGCCTGTTGCAAAGCAATGGATAGTTATGTTTCCCAGTTGCAAGAGCAGAGCTTTATTACAAACTTGCAGGCCTTAAATTGTGCTGCATCACTTGGAGTGAAGTTGCAGAAAGCTAATGTATCCAATAATGTTTATCAACTTTGTCACATAAACCTCAAGGACTTCTCTCTTCAAGTTGGATCACAAGAGTATGGCTGCCTTCTGCCAAGCCTGCCTTCAGATGCAACATTTGACAAAACTTCAGGAGTCAGCTTCATTTGTGATCTTAATGACAACATTGCAGCTCCGTGGCCCTCTTCAATTTCTGAACCTCCATCGTCCTGCAACAGAATAAAATCCCTAGTATATATATGTATGAGCAGCTCCATACAAGTTTCTGAGGCAGAGGTATTGGTGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

431

Amino Acids

46.38

Weight (kDa)

5.28

Isoelectric Point (pI)

56.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPARK PF19160 73 - 224 1e-22 SPARK
At1g61900 PF26584 274 - 347 2.4e-34 At1g61900-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 430, 584, 761, 1081
AcsI RAATTY 2 cut(s) 235, 676
AcuI CTGAAG 3 cut(s) 525, 1095, 1119
AcvI CACGTG 1 cut(s) 854
AfaI GTAC 2 cut(s) 229, 699
AfiI CCNNNNNNNGG 2 cut(s) 81, 282
AflIII ACRYGT 2 cut(s) 851, 853
AgsI TTSAA 8 cut(s) 235, 394, 470, 488, 561, 804, 1067, 1194
AjnI CCWGG 3 cut(s) 25, 713, 888
AjuI GAANNNNNNNTTGG 2 cut(s) 1093, 1125
AluBI AGCT 8 cut(s) 245, 331, 509, 945, 1007, 1146, 1178, 1257
AluI AGCT 8 cut(s) 245, 331, 509, 945, 1007, 1146, 1178, 1257
Alw26I GTCTC 2 cut(s) 19, 62
AlwI GGATC 4 cut(s) 430, 584, 761, 1081
AlwNI CAGNNNCTG 1 cut(s) 60
AoxI GGCC 4 cut(s) 285, 336, 963, 1184
ApoI RAATTY 2 cut(s) 235, 676
AspLEI GCGC 1 cut(s) 298
AspS9I GGNCC 5 cut(s) 23, 285, 688, 787, 1185
AsuHPI GGTGA 1 cut(s) 159
AsuNHI GCTAGC 1 cut(s) 751
AvaII GGWCC 3 cut(s) 23, 688, 787
BaeGI GKGCMC 1 cut(s) 363
BanII GRGCYC 1 cut(s) 33
BbrPI CACGTG 1 cut(s) 854
BbvCI CCTCAGC 1 cut(s) 327
BccI CCATC 2 cut(s) 741, 1216
BciT130I CCWGG 3 cut(s) 27, 715, 890
BciVI GTATCC 1 cut(s) 1024
BcoDI GTCTC 2 cut(s) 19, 62
BfaI CTAG 5 cut(s) 378, 440, 645, 752, 1235
BfuI GTATCC 1 cut(s) 1024
Bme1390I CCNGG 3 cut(s) 27, 715, 890
Bme18I GGWCC 3 cut(s) 23, 688, 787
BmgT120I GGNCC 5 cut(s) 23, 285, 688, 787, 1185
BmiI GGNNCC 2 cut(s) 32, 689
BmrFI CCNGG 3 cut(s) 27, 715, 890
BmrI ACTGGG 1 cut(s) 922
BmsI GCATC 5 cut(s) 372, 523, 628, 989, 1105
BmtI GCTAGC 1 cut(s) 755
BmuI ACTGGG 1 cut(s) 922
BpmI CTGGAG 1 cut(s) 349
Bpu10I CCTNAGC 1 cut(s) 327
BpuEI CTTGAG 2 cut(s) 353, 1034
BsaAI YACGTR 1 cut(s) 854
BsaI GGTCTC 1 cut(s) 19
BsaJI CCNNGG 2 cut(s) 26, 1181
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 282
Bse1I ACTGG 4 cut(s) 126, 249, 366, 928
Bse3DI GCAATG 2 cut(s) 915, 1170
BseBI CCWGG 3 cut(s) 27, 715, 890
BseDI CCNNGG 2 cut(s) 26, 1181
BseGI GGATG 2 cut(s) 122, 387
BseLI CCNNNNNNNGG 2 cut(s) 81, 282
BseMI GCAATG 2 cut(s) 915, 1170
BseMII CTCAG 5 cut(s) 60, 341, 435, 648, 1263
BseNI ACTGG 4 cut(s) 126, 249, 366, 928
BseSI GKGCMC 1 cut(s) 363
BseYI CCCAGC 1 cut(s) 282
BshFI GGCC 4 cut(s) 287, 338, 965, 1186
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 2 cut(s) 81, 282
BsmAI GTCTC 2 cut(s) 19, 62
BsmFI GGGAC 1 cut(s) 58
BsmI GAATGC 1 cut(s) 204
BsnI GGCC 4 cut(s) 287, 338, 965, 1186
Bso31I GGTCTC 1 cut(s) 19
Bsp1286I GDGCHC 2 cut(s) 33, 363
Bsp1407I TGTACA 1 cut(s) 227
Bsp143I GATC 5 cut(s) 435, 589, 766, 1073, 1156
BspANI GGCC 4 cut(s) 287, 338, 965, 1186
BspCNI CTCAG 5 cut(s) 61, 340, 434, 649, 1264
BspLI GGNNCC 2 cut(s) 32, 689
BspOI GCTAGC 1 cut(s) 755
BspPI GGATC 4 cut(s) 430, 584, 761, 1081
BspTNI GGTCTC 1 cut(s) 19
BsrDI GCAATG 2 cut(s) 915, 1170
BsrGI TGTACA 1 cut(s) 227
BsrI ACTGG 4 cut(s) 126, 249, 366, 928
BssECI CCNNGG 2 cut(s) 26, 1181
BssMI GATC 5 cut(s) 435, 589, 766, 1073, 1156
Bst2UI CCWGG 3 cut(s) 27, 715, 890
Bst4CI ACNGT 2 cut(s) 466, 708
Bst6I CTCTTC 2 cut(s) 1068, 1195
BstAUI TGTACA 1 cut(s) 227
BstBAI YACGTR 1 cut(s) 854
BstC8I GCNNGC 4 cut(s) 577, 753, 963, 1106
BstDEI CTNAG 6 cut(s) 69, 327, 421, 657, 791, 1272
BstDSI CCRYGG 1 cut(s) 1181
BstF5I GGATG 2 cut(s) 122, 387
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 5 cut(s) 438, 592, 769, 1076, 1159
BstMAI GTCTC 2 cut(s) 19, 62
BstMBI GATC 5 cut(s) 435, 589, 766, 1073, 1156
BstMWI GCNNNNNNNGC 3 cut(s) 293, 748, 1105
BstNI CCWGG 3 cut(s) 27, 715, 890
BstSCI CCNGG 3 cut(s) 25, 713, 888
BstSLI GKGCMC 1 cut(s) 363
BstX2I RGATCY 1 cut(s) 435
BstYI RGATCY 1 cut(s) 435
BsuI GTATCC 1 cut(s) 1024
BsuRI GGCC 4 cut(s) 287, 338, 965, 1186
BtgI CCRYGG 1 cut(s) 1181
BtgZI GCGATG 2 cut(s) 108, 760
BtsCI GGATG 2 cut(s) 122, 387
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 4 cut(s) 133, 256, 412, 501
Cac8I GCNNGC 4 cut(s) 577, 753, 963, 1106
CaiI CAGNNNCTG 1 cut(s) 60
CfoI GCGC 1 cut(s) 298
Cfr13I GGNCC 5 cut(s) 23, 285, 688, 787, 1185
Csp6I GTAC 2 cut(s) 228, 698
CviAII CATG 2 cut(s) 404, 847
CviQI GTAC 2 cut(s) 228, 698
DdeI CTNAG 6 cut(s) 69, 327, 421, 657, 791, 1272
DpnI GATC 5 cut(s) 437, 591, 768, 1075, 1158
DpnII GATC 5 cut(s) 435, 589, 766, 1073, 1156
Eam1104I CTCTTC 2 cut(s) 1068, 1195
EarI CTCTTC 2 cut(s) 1068, 1195
Eco147I AGGCCT 1 cut(s) 965
Eco24I GRGCYC 1 cut(s) 33
Eco31I GGTCTC 1 cut(s) 19
Eco32I GATATC 1 cut(s) 118
Eco47I GGWCC 3 cut(s) 23, 688, 787
Eco57I CTGAAG 3 cut(s) 525, 1095, 1119
Eco72I CACGTG 1 cut(s) 854
EcoO109I RGGNCCY 1 cut(s) 787
EcoRII CCWGG 3 cut(s) 25, 713, 888
EcoRV GATATC 1 cut(s) 118
EcoT38I GRGCYC 1 cut(s) 33
FaeI CATG 2 cut(s) 407, 850
FalI AAGNNNNNCTT 2 cut(s) 1093, 1125
FaqI GGGAC 1 cut(s) 58
FatI CATG 2 cut(s) 403, 846
FokI GGATG 2 cut(s) 109, 394
FriOI GRGCYC 1 cut(s) 33
FspBI CTAG 5 cut(s) 378, 440, 645, 752, 1235
GlaI GCGC 1 cut(s) 297
GsaI CCCAGC 1 cut(s) 286
GsuI CTGGAG 1 cut(s) 349
HaeIII GGCC 4 cut(s) 287, 338, 965, 1186
HhaI GCGC 1 cut(s) 298
Hin1II CATG 2 cut(s) 407, 850
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HincII GTYRAC 1 cut(s) 711
HindII GTYRAC 1 cut(s) 711
HindIII AAGCTT 1 cut(s) 507
HinfI GANTC 5 cut(s) 71, 222, 445, 554, 1140
HphI GGTGA 1 cut(s) 159
Hpy166II GTNNAC 2 cut(s) 133, 711
Hpy188I TCNGA 6 cut(s) 70, 210, 424, 1114, 1201, 1273
Hpy188III TCNNGA 5 cut(s) 113, 219, 449, 611, 1137
Hpy8I GTNNAC 2 cut(s) 133, 711
HpyAV CCTTC 5 cut(s) 174, 677, 780, 1103, 1119
HpyCH4III ACNGT 2 cut(s) 466, 708
HpyCH4IV ACGT 1 cut(s) 853
HpyF10VI GCNNNNNNNGC 3 cut(s) 293, 748, 1105
HpyF3I CTNAG 6 cut(s) 69, 327, 421, 657, 791, 1272
HpySE526I ACGT 1 cut(s) 853
Hsp92II CATG 2 cut(s) 407, 850
HspAI GCGC 1 cut(s) 296
Kzo9I GATC 5 cut(s) 435, 589, 766, 1073, 1156
LmnI GCTCC 4 cut(s) 36, 115, 1183, 1262
LweI GCATC 5 cut(s) 372, 523, 628, 989, 1105
MaeI CTAG 5 cut(s) 378, 440, 645, 752, 1235
MaeII ACGT 1 cut(s) 853
MaeIII GTNAC 3 cut(s) 520, 758, 1037
MalI GATC 5 cut(s) 437, 591, 768, 1075, 1158
MboI GATC 5 cut(s) 435, 589, 766, 1073, 1156
MboII GAAGA 5 cut(s) 445, 549, 765, 1055, 1182
MfeI CAATTG 1 cut(s) 105
MflI RGATCY 1 cut(s) 435
MhlI GDGCHC 2 cut(s) 33, 363
MluCI AATT 7 cut(s) 105, 235, 483, 676, 805, 970, 1194
MlyI GAGTC 4 cut(s) 80, 216, 454, 1149
MmeI TCCRAC 2 cut(s) 233, 1051
MseI TTAA 2 cut(s) 968, 1161
MspA1I CMGCKG 1 cut(s) 245
MspR9I CCNGG 3 cut(s) 27, 715, 890
MunI CAATTG 1 cut(s) 105
Mva1269I GAATGC 1 cut(s) 204
MvaI CCWGG 3 cut(s) 27, 715, 890
MwoI GCNNNNNNNGC 3 cut(s) 293, 748, 1105
NdeII GATC 5 cut(s) 435, 589, 766, 1073, 1156
NheI GCTAGC 1 cut(s) 751
NlaIII CATG 2 cut(s) 407, 850
NlaIV GGNNCC 2 cut(s) 32, 689
NmuCI GTSAC 1 cut(s) 1037
PceI AGGCCT 1 cut(s) 965
PctI GAATGC 1 cut(s) 204
PfeI GAWTC 1 cut(s) 554
PleI GAGTC 4 cut(s) 79, 216, 453, 1148
PmaCI CACGTG 1 cut(s) 854
PmlI CACGTG 1 cut(s) 854
PpsI GAGTC 4 cut(s) 79, 216, 453, 1148
Ppu21I YACGTR 1 cut(s) 854
PpuMI RGGWCCY 1 cut(s) 787
Psp5II RGGWCCY 1 cut(s) 787
Psp6I CCWGG 3 cut(s) 25, 713, 888
PspCI CACGTG 1 cut(s) 854
PspFI CCCAGC 1 cut(s) 282
PspGI CCWGG 3 cut(s) 25, 713, 888
PspN4I GGNNCC 2 cut(s) 32, 689
PspPI GGNCC 5 cut(s) 23, 285, 688, 787, 1185
PspPPI RGGWCCY 1 cut(s) 787
PstNI CAGNNNCTG 1 cut(s) 60
PsuI RGATCY 1 cut(s) 435
PvuII CAGCTG 1 cut(s) 245
RsaI GTAC 2 cut(s) 229, 699
RsaNI GTAC 2 cut(s) 228, 698
SaqAI TTAA 2 cut(s) 968, 1161
Sau3AI GATC 5 cut(s) 435, 589, 766, 1073, 1156
Sau96I GGNCC 5 cut(s) 23, 285, 688, 787, 1185
SchI GAGTC 4 cut(s) 80, 216, 454, 1149
ScrFI CCNGG 3 cut(s) 27, 715, 890
SduI GDGCHC 2 cut(s) 33, 363
SfaNI GCATC 5 cut(s) 372, 523, 628, 989, 1105
SinI GGWCC 3 cut(s) 23, 688, 787
SmlI CTYRAG 2 cut(s) 332, 1049
SmoI CTYRAG 2 cut(s) 332, 1049
Sse9I AATT 7 cut(s) 105, 235, 483, 676, 805, 970, 1194
SseBI AGGCCT 1 cut(s) 965
SspI AATATT 1 cut(s) 736
SspMI CTAG 5 cut(s) 378, 440, 645, 752, 1235
StuI AGGCCT 1 cut(s) 965
StyD4I CCNGG 3 cut(s) 25, 713, 888
TaaI ACNGT 2 cut(s) 466, 708
TaiI ACGT 1 cut(s) 856
TasI AATT 7 cut(s) 105, 235, 483, 676, 805, 970, 1194
TatI WGTACW 1 cut(s) 227
TfiI GAWTC 1 cut(s) 554
Tru1I TTAA 2 cut(s) 968, 1161
Tru9I TTAA 2 cut(s) 968, 1161
TscAI CASTG 4 cut(s) 133, 256, 419, 508
TseFI GTSAC 1 cut(s) 1037
Tsp45I GTSAC 1 cut(s) 1037
TspDTI ATGAA 1 cut(s) 1138
TspGWI ACGGA 2 cut(s) 685, 1170
TspRI CASTG 4 cut(s) 133, 256, 419, 508
VpaK11BI GGWCC 3 cut(s) 23, 688, 787
XapI RAATTY 2 cut(s) 235, 676
XspI CTAG 5 cut(s) 378, 440, 645, 752, 1235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.