Rw2G044900

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0011585
Physical Location & Seq
Forward (+)
71722024 .. 71725400
3377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G044900.1

Sequence Viewer

Length: 1329 bp
ATGAGGCAGAGAGTCTCTTATGGTCCAGGCCTCCTTACTCTTACTCTACTTTTCAGTCTCTGTGTATATGAGTCCCAATGTGGGTCAGTTGGCAATGTGGAGGCAATTGCTCCTGATATCTCATCCAGTGTGAACCCTCAACCTTTTCTGCCTTTATTAGCACCTTCACCATTGACACCATTCACAAATGACAGCATTCCGACATTATCAGGACTCTGTACATTGAATTTTACAGCTGCCAGTGATATGATGAGCAAAACAGCAACCGACTGCTGGGCCTTTTTTGCGCCCGTATTGGCTAATGTAGTGTGTTGCCCTCAGCTTGAGGCCACCTTGACCACTCTTATAGGGCACTCCAGTAAATACTCTAGGATGCTCTCTTTGAATATAACTCATGCAAAGCACTGCCTCTCAGATGTTGAGAAGATCCTAGAGAGTCAAGGAGCCAATAAGACTGTTCAAAAGATTTGCTCAATTCAACCAAAAAACCTCACTCAAGCTTCTTGCCCTGTTACAAAGGTTGATGCGTTTGAGAGCATTGTGGATTCTTCAAAACTACTTGCTGCTTGCCAAAAAGTTGATCCTGTCAATGAGTGTTGTCAGGAAGTTTGCCAAAATGCCATATTAGATGCTGCTAGAAAACTTGCTGAGATTGGTATTGCTGCAAATTTGGAAGGGGTCCATTCCGTACCTGAACAGTCAACCAGGATAGATGATTGTAAGAATATTGTGCTGCGATGGCTAGCGAGTAACCTTGATCCTTCTTCTGCAAATATGGTACTTAGAGGACTTTCAAATTGCAAAGTAAATAAAGTGTGTCCTCTTATTTTCCCCAACATGACACGTGTTGTGAAAGAATGTGCAAATATGGTAACAAGCCAGGCTGCCTGTTGCAAAGCAATGGATAGTTATGTTTCCCAGTTGCAAGAGCAGAGCTTTATTACAAACTTGCAGGCCTTAAATTGTGCTGCATCACTTGGAGTGAAGTTGCAGAAAGCTAATGTATCCAATAATGTTTATCAACTTTGTCACATAAACCTCAAGGACTTCTCTCTTCAAGTTGGATCACAAGAGTATGGCTGCCTTCTGCCAAGCCTGCCTTCAGATGCAACATTTGACAAAACTTCAGGAGTCAGCTTCATTTGTGATCTTAATGACAACATTGCAGCTCCGTGGCCCTCTTCAATTTCTGAACCTCCATCGTCCTGCAACAGAACTACCAAAATCCCAGCAGTACCCAAAGCAACATCTGCACAAAGTGGTCTTTACAGGAACTTGATGCTCCCTTCCTTATTTTCCTCCTTGGTGATTCTCAAGCCGCTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

442

Amino Acids

47.51

Weight (kDa)

6.88

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPARK PF19160 70 - 221 1.1e-22 SPARK
At1g61900 PF26584 271 - 344 5.7e-34 At1g61900-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 1319
AclWI GGATC 4 cut(s) 421, 575, 752, 1072
AcsI RAATTY 2 cut(s) 226, 667
AcuI CTGAAG 2 cut(s) 1086, 1110
AcvI CACGTG 1 cut(s) 845
AdeI CACNNNGTG 1 cut(s) 1259
AfaI GTAC 4 cut(s) 220, 690, 780, 1236
AfiI CCNNNNNNNGG 2 cut(s) 81, 273
AflIII ACRYGT 2 cut(s) 842, 844
AgsI TTSAA 8 cut(s) 226, 385, 461, 479, 552, 795, 1058, 1185
AjnI CCWGG 3 cut(s) 25, 704, 879
AjuI GAANNNNNNNTTGG 2 cut(s) 1084, 1116
AluBI AGCT 7 cut(s) 236, 322, 500, 936, 998, 1137, 1169
AluI AGCT 7 cut(s) 236, 322, 500, 936, 998, 1137, 1169
Alw26I GTCTC 2 cut(s) 19, 62
AlwI GGATC 4 cut(s) 421, 575, 752, 1072
AlwNI CAGNNNCTG 1 cut(s) 60
AoxI GGCC 5 cut(s) 28, 276, 327, 954, 1175
ApeKI GCWGC 9 cut(s) 236, 563, 632, 662, 733, 884, 968, 1080, 1166
ApoI RAATTY 2 cut(s) 226, 667
AspLEI GCGC 1 cut(s) 289
AspS9I GGNCC 4 cut(s) 23, 276, 679, 1176
AsuHPI GGTGA 2 cut(s) 159, 1318
AsuNHI GCTAGC 1 cut(s) 742
AvaII GGWCC 2 cut(s) 23, 679
BaeGI GKGCMC 1 cut(s) 354
BbrPI CACGTG 1 cut(s) 845
BbvCI CCTCAGC 1 cut(s) 318
BbvI GCAGC 9 cut(s) 223, 550, 619, 649, 720, 871, 955, 1067, 1178
BccI CCATC 2 cut(s) 732, 1207
BciT130I CCWGG 3 cut(s) 27, 706, 881
BciVI GTATCC 1 cut(s) 1015
BcoDI GTCTC 2 cut(s) 19, 62
BfaI CTAG 4 cut(s) 369, 431, 636, 743
BfuI GTATCC 1 cut(s) 1015
Bme1390I CCNGG 3 cut(s) 27, 706, 881
Bme18I GGWCC 2 cut(s) 23, 679
BmgT120I GGNCC 4 cut(s) 23, 276, 679, 1176
BmiI GGNNCC 2 cut(s) 445, 680
BmrFI CCNGG 3 cut(s) 27, 706, 881
BmrI ACTGGG 1 cut(s) 913
BmsI GCATC 6 cut(s) 363, 514, 619, 980, 1096, 1269
BmtI GCTAGC 1 cut(s) 746
BmuI ACTGGG 1 cut(s) 913
BpmI CTGGAG 1 cut(s) 340
Bpu10I CCTNAGC 1 cut(s) 318
BpuEI CTTGAG 4 cut(s) 344, 480, 1025, 1298
BsaAI YACGTR 1 cut(s) 845
BsaJI CCNNGG 2 cut(s) 1172, 1302
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 273
Bse1I ACTGG 4 cut(s) 126, 240, 357, 919
Bse3DI GCAATG 3 cut(s) 100, 906, 1161
BseBI CCWGG 3 cut(s) 27, 706, 881
BseDI CCNNGG 2 cut(s) 1172, 1302
BseGI GGATG 2 cut(s) 122, 378
BseLI CCNNNNNNNGG 2 cut(s) 81, 273
BseMI GCAATG 3 cut(s) 100, 906, 1161
BseMII CTCAG 3 cut(s) 332, 426, 639
BseNI ACTGG 4 cut(s) 126, 240, 357, 919
BseSI GKGCMC 1 cut(s) 354
BseXI GCAGC 9 cut(s) 223, 550, 619, 649, 720, 871, 955, 1067, 1178
BseYI CCCAGC 2 cut(s) 273, 1228
BsgI GTGCAG 1 cut(s) 1236
BshFI GGCC 5 cut(s) 30, 278, 329, 956, 1177
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 2 cut(s) 81, 273
BsmAI GTCTC 2 cut(s) 19, 62
BsmFI GGGAC 1 cut(s) 58
BsmI GAATGC 1 cut(s) 195
BsnI GGCC 5 cut(s) 30, 278, 329, 956, 1177
Bsp1286I GDGCHC 1 cut(s) 354
Bsp1407I TGTACA 1 cut(s) 218
Bsp143I GATC 5 cut(s) 426, 580, 757, 1064, 1147
BspACI CCGC 1 cut(s) 1319
BspANI GGCC 5 cut(s) 30, 278, 329, 956, 1177
BspCNI CTCAG 3 cut(s) 331, 425, 640
BspLI GGNNCC 2 cut(s) 445, 680
BspOI GCTAGC 1 cut(s) 746
BspPI GGATC 4 cut(s) 421, 575, 752, 1072
BsrDI GCAATG 3 cut(s) 100, 906, 1161
BsrGI TGTACA 1 cut(s) 218
BsrI ACTGG 4 cut(s) 126, 240, 357, 919
BssECI CCNNGG 2 cut(s) 1172, 1302
BssMI GATC 5 cut(s) 426, 580, 757, 1064, 1147
BssT1I CCWWGG 1 cut(s) 1302
Bst2UI CCWGG 3 cut(s) 27, 706, 881
Bst4CI ACNGT 2 cut(s) 457, 699
Bst6I CTCTTC 2 cut(s) 1059, 1186
BstAPI GCANNNNNTGC 1 cut(s) 1250
BstAUI TGTACA 1 cut(s) 218
BstBAI YACGTR 1 cut(s) 845
BstC8I GCNNGC 4 cut(s) 568, 744, 954, 1097
BstDEI CTNAG 4 cut(s) 318, 412, 648, 782
BstDSI CCRYGG 1 cut(s) 1172
BstF5I GGATG 2 cut(s) 122, 378
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 5 cut(s) 429, 583, 760, 1067, 1150
BstMAI GTCTC 2 cut(s) 19, 62
BstMBI GATC 5 cut(s) 426, 580, 757, 1064, 1147
BstMWI GCNNNNNNNGC 4 cut(s) 284, 739, 1096, 1250
BstNI CCWGG 3 cut(s) 27, 706, 881
BstSCI CCNGG 3 cut(s) 25, 704, 879
BstSLI GKGCMC 1 cut(s) 354
BstV1I GCAGC 9 cut(s) 223, 550, 619, 649, 720, 871, 955, 1067, 1178
BstX2I RGATCY 1 cut(s) 426
BstYI RGATCY 1 cut(s) 426
BsuI GTATCC 1 cut(s) 1015
BsuRI GGCC 5 cut(s) 30, 278, 329, 956, 1177
BtgI CCRYGG 1 cut(s) 1172
BtgZI GCGATG 1 cut(s) 751
BtsCI GGATG 2 cut(s) 122, 378
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 3 cut(s) 133, 247, 403
Cac8I GCNNGC 4 cut(s) 568, 744, 954, 1097
CaiI CAGNNNCTG 1 cut(s) 60
CfoI GCGC 1 cut(s) 289
Cfr13I GGNCC 4 cut(s) 23, 276, 679, 1176
Csp6I GTAC 4 cut(s) 219, 689, 779, 1235
CviAII CATG 2 cut(s) 395, 838
CviQI GTAC 4 cut(s) 219, 689, 779, 1235
DdeI CTNAG 4 cut(s) 318, 412, 648, 782
DpnI GATC 5 cut(s) 428, 582, 759, 1066, 1149
DpnII GATC 5 cut(s) 426, 580, 757, 1064, 1147
DraIII CACNNNGTG 1 cut(s) 1259
Eam1104I CTCTTC 2 cut(s) 1059, 1186
EarI CTCTTC 2 cut(s) 1059, 1186
Eco130I CCWWGG 1 cut(s) 1302
Eco147I AGGCCT 2 cut(s) 30, 956
Eco32I GATATC 1 cut(s) 118
Eco47I GGWCC 2 cut(s) 23, 679
Eco57I CTGAAG 2 cut(s) 1086, 1110
Eco72I CACGTG 1 cut(s) 845
EcoRII CCWGG 3 cut(s) 25, 704, 879
EcoRV GATATC 1 cut(s) 118
EcoT14I CCWWGG 1 cut(s) 1302
ErhI CCWWGG 1 cut(s) 1302
FaeI CATG 2 cut(s) 398, 841
FalI AAGNNNNNCTT 2 cut(s) 1084, 1116
FaqI GGGAC 1 cut(s) 58
FatI CATG 2 cut(s) 394, 837
FokI GGATG 2 cut(s) 109, 385
FspBI CTAG 4 cut(s) 369, 431, 636, 743
GlaI GCGC 1 cut(s) 288
GsaI CCCAGC 2 cut(s) 277, 1232
GsuI CTGGAG 1 cut(s) 340
HaeIII GGCC 5 cut(s) 30, 278, 329, 956, 1177
HhaI GCGC 1 cut(s) 289
Hin1II CATG 2 cut(s) 398, 841
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HincII GTYRAC 1 cut(s) 702
HindII GTYRAC 1 cut(s) 702
HindIII AAGCTT 1 cut(s) 498
HinfI GANTC 7 cut(s) 12, 71, 213, 436, 545, 1131, 1309
HphI GGTGA 2 cut(s) 159, 1318
Hpy166II GTNNAC 2 cut(s) 133, 702
Hpy188I TCNGA 5 cut(s) 201, 415, 1105, 1192, 1328
Hpy188III TCNNGA 4 cut(s) 113, 210, 602, 1128
Hpy8I GTNNAC 2 cut(s) 133, 702
HpyAV CCTTC 6 cut(s) 174, 668, 771, 1094, 1110, 1296
HpyCH4III ACNGT 2 cut(s) 457, 699
HpyCH4IV ACGT 1 cut(s) 844
HpyF10VI GCNNNNNNNGC 4 cut(s) 284, 739, 1096, 1250
HpyF3I CTNAG 4 cut(s) 318, 412, 648, 782
HpySE526I ACGT 1 cut(s) 844
Hsp92II CATG 2 cut(s) 398, 841
HspAI GCGC 1 cut(s) 287
Kzo9I GATC 5 cut(s) 426, 580, 757, 1064, 1147
LmnI GCTCC 4 cut(s) 115, 443, 1174, 1287
Lsp1109I GCAGC 9 cut(s) 223, 550, 619, 649, 720, 871, 955, 1067, 1178
LweI GCATC 6 cut(s) 363, 514, 619, 980, 1096, 1269
MaeI CTAG 4 cut(s) 369, 431, 636, 743
MaeII ACGT 1 cut(s) 844
MaeIII GTNAC 4 cut(s) 511, 749, 871, 1028
MalI GATC 5 cut(s) 428, 582, 759, 1066, 1149
MboI GATC 5 cut(s) 426, 580, 757, 1064, 1147
MboII GAAGA 5 cut(s) 436, 540, 756, 1046, 1173
MfeI CAATTG 1 cut(s) 105
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 1 cut(s) 354
MluCI AATT 7 cut(s) 105, 226, 474, 667, 796, 961, 1185
MlyI GAGTC 5 cut(s) 21, 80, 207, 445, 1140
MmeI TCCRAC 2 cut(s) 224, 1042
MseI TTAA 2 cut(s) 959, 1152
MspA1I CMGCKG 1 cut(s) 236
MspR9I CCNGG 3 cut(s) 27, 706, 881
MunI CAATTG 1 cut(s) 105
Mva1269I GAATGC 1 cut(s) 195
MvaI CCWGG 3 cut(s) 27, 706, 881
MwoI GCNNNNNNNGC 4 cut(s) 284, 739, 1096, 1250
NdeII GATC 5 cut(s) 426, 580, 757, 1064, 1147
NheI GCTAGC 1 cut(s) 742
NlaIII CATG 2 cut(s) 398, 841
NlaIV GGNNCC 2 cut(s) 445, 680
NmuCI GTSAC 1 cut(s) 1028
PceI AGGCCT 2 cut(s) 30, 956
PctI GAATGC 1 cut(s) 195
PfeI GAWTC 2 cut(s) 545, 1309
PleI GAGTC 5 cut(s) 20, 79, 207, 444, 1139
PmaCI CACGTG 1 cut(s) 845
PmlI CACGTG 1 cut(s) 845
PpsI GAGTC 5 cut(s) 20, 79, 207, 444, 1139
Ppu21I YACGTR 1 cut(s) 845
Psp6I CCWGG 3 cut(s) 25, 704, 879
PspCI CACGTG 1 cut(s) 845
PspFI CCCAGC 2 cut(s) 273, 1228
PspGI CCWGG 3 cut(s) 25, 704, 879
PspN4I GGNNCC 2 cut(s) 445, 680
PspPI GGNCC 4 cut(s) 23, 276, 679, 1176
PstNI CAGNNNCTG 1 cut(s) 60
PsuI RGATCY 1 cut(s) 426
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 4 cut(s) 220, 690, 780, 1236
RsaNI GTAC 4 cut(s) 219, 689, 779, 1235
SaqAI TTAA 2 cut(s) 959, 1152
Sau3AI GATC 5 cut(s) 426, 580, 757, 1064, 1147
Sau96I GGNCC 4 cut(s) 23, 276, 679, 1176
SchI GAGTC 5 cut(s) 21, 80, 207, 445, 1140
ScrFI CCNGG 3 cut(s) 27, 706, 881
SduI GDGCHC 1 cut(s) 354
SfaNI GCATC 6 cut(s) 363, 514, 619, 980, 1096, 1269
SinI GGWCC 2 cut(s) 23, 679
SmlI CTYRAG 4 cut(s) 323, 495, 1040, 1313
SmoI CTYRAG 4 cut(s) 323, 495, 1040, 1313
Sse9I AATT 7 cut(s) 105, 226, 474, 667, 796, 961, 1185
SseBI AGGCCT 2 cut(s) 30, 956
SsiI CCGC 1 cut(s) 1319
SspI AATATT 1 cut(s) 727
SspMI CTAG 4 cut(s) 369, 431, 636, 743
StuI AGGCCT 2 cut(s) 30, 956
StyD4I CCNGG 3 cut(s) 25, 704, 879
StyI CCWWGG 1 cut(s) 1302
TaaI ACNGT 2 cut(s) 457, 699
TaiI ACGT 1 cut(s) 847
TasI AATT 7 cut(s) 105, 226, 474, 667, 796, 961, 1185
TatI WGTACW 1 cut(s) 218
TauI GCSGC 1 cut(s) 1321
TfiI GAWTC 2 cut(s) 545, 1309
Tru1I TTAA 2 cut(s) 959, 1152
Tru9I TTAA 2 cut(s) 959, 1152
TscAI CASTG 3 cut(s) 133, 247, 410
TseFI GTSAC 1 cut(s) 1028
TseI GCWGC 9 cut(s) 236, 563, 632, 662, 733, 884, 968, 1080, 1166
Tsp45I GTSAC 1 cut(s) 1028
TspDTI ATGAA 1 cut(s) 1129
TspGWI ACGGA 2 cut(s) 676, 1161
TspRI CASTG 3 cut(s) 133, 247, 410
VpaK11BI GGWCC 2 cut(s) 23, 679
XapI RAATTY 2 cut(s) 226, 667
XspI CTAG 4 cut(s) 369, 431, 636, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.