MD17G1096500.v1.1

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011585
Physical Location & Seq
Reverse (-)
8149286 .. 8151354
2069 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1096500.v1.1.491

Sequence Viewer

Length: 369 bp
ATGCTGACTTATAAATCTTCAACGTATGGTTCAATGTGTCCTTTGGTTTTCCCCAACATGACAAATGTTGTGAAAGAATGTGCAAATGTGATAAGCAATGAGACAGCGTGCTGCAGTGCGATGGATATATGGCTGCCTTCTGCTAGCTTGCCTTTGGATGCGACTTATGATAAAACTTCAGAAATCAGCTTCATTTGTGATCTTAACAACAATATTGCGGCTCCATGGCCGTCTTTGTCTTTTGAATCTCCACCATCTTGCAAGAAGAGTATGCAAGAAATGTGTTTATTTTTTACTCTCTTTTTTCTATGCTCCTTATGTTTTTTTTTTTATTTTACAGCCACACATTTTACACTGCTTTTCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

123

Amino Acids

13.75

Weight (kDa)

4.6

Isoelectric Point (pI)

53.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At1g61900 PF26584 12 - 44 8e-11 At1g61900-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 12
AciI CCGC 1 cut(s) 218
AcoI YGGCCR 1 cut(s) 227
AcuI CTGAAG 1 cut(s) 162
AgsI TTSAA 3 cut(s) 21, 33, 245
AluBI AGCT 2 cut(s) 147, 189
AluI AGCT 2 cut(s) 147, 189
Alw26I GTCTC 1 cut(s) 95
AoxI GGCC 1 cut(s) 227
ApeKI GCWGC 2 cut(s) 111, 133
AsuNHI GCTAGC 1 cut(s) 143
BbvI GCAGC 2 cut(s) 98, 120
BccI CCATC 2 cut(s) 115, 262
BceAI ACGGC 1 cut(s) 214
BcoDI GTCTC 1 cut(s) 95
BfaI CTAG 1 cut(s) 144
BfmI CTRYAG 1 cut(s) 112
BisI GCNGC 3 cut(s) 112, 134, 219
BlsI GCNGC 3 cut(s) 113, 135, 220
BmiI GGNNCC 1 cut(s) 222
BmsI GCATC 1 cut(s) 148
BmtI GCTAGC 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 224
Bse3DI GCAATG 1 cut(s) 103
BseDI CCNNGG 1 cut(s) 224
BseGI GGATG 1 cut(s) 163
BseMI GCAATG 1 cut(s) 103
BseXI GCAGC 2 cut(s) 98, 120
BshFI GGCC 1 cut(s) 229
BsmAI GTCTC 1 cut(s) 95
BsnI GGCC 1 cut(s) 229
Bsp143I GATC 1 cut(s) 199
Bsp19I CCATGG 1 cut(s) 224
BspACI CCGC 1 cut(s) 218
BspANI GGCC 1 cut(s) 229
BspLI GGNNCC 1 cut(s) 222
BspMAI CTGCAG 1 cut(s) 116
BspOI GCTAGC 1 cut(s) 147
BsrDI GCAATG 1 cut(s) 103
BssECI CCNNGG 1 cut(s) 224
BssMI GATC 1 cut(s) 199
BssT1I CCWWGG 1 cut(s) 224
Bst6I CTCTTC 1 cut(s) 260
BstC8I GCNNGC 3 cut(s) 109, 145, 149
BstDSI CCRYGG 1 cut(s) 224
BstF5I GGATG 1 cut(s) 163
BstKTI GATC 1 cut(s) 202
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 1 cut(s) 199
BstSFI CTRYAG 1 cut(s) 112
BstV1I GCAGC 2 cut(s) 98, 120
BsuRI GGCC 1 cut(s) 229
BtgI CCRYGG 1 cut(s) 224
BtgZI GCGATG 1 cut(s) 134
BtsCI GGATG 1 cut(s) 163
BtsI GCAGTG 2 cut(s) 121, 353
BtsIMutI CAGTG 2 cut(s) 121, 353
Cac8I GCNNGC 3 cut(s) 109, 145, 149
CviAII CATG 2 cut(s) 58, 225
CviJI RGCY 6 cut(s) 133, 147, 189, 221, 229, 341
CviKI_1 RGCY 6 cut(s) 133, 147, 189, 221, 229, 341
DpnI GATC 1 cut(s) 201
DpnII GATC 1 cut(s) 199
EaeI YGGCCR 1 cut(s) 227
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
Eco130I CCWWGG 1 cut(s) 224
Eco57I CTGAAG 1 cut(s) 162
EcoT14I CCWWGG 1 cut(s) 224
ErhI CCWWGG 1 cut(s) 224
FaeI CATG 2 cut(s) 61, 228
FatI CATG 2 cut(s) 57, 224
Fnu4HI GCNGC 3 cut(s) 112, 134, 219
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 3 cut(s) 112, 134, 219
FspBI CTAG 1 cut(s) 144
GluI GCNGC 3 cut(s) 112, 134, 219
HaeIII GGCC 1 cut(s) 229
Hin1II CATG 2 cut(s) 61, 228
HinfI GANTC 1 cut(s) 245
Hpy188I TCNGA 1 cut(s) 181
HpyAV CCTTC 1 cut(s) 147
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 4 cut(s) 83, 114, 261, 274
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 2 cut(s) 61, 228
Kzo9I GATC 1 cut(s) 199
LmnI GCTCC 2 cut(s) 226, 317
Lsp1109I GCAGC 2 cut(s) 98, 120
LweI GCATC 1 cut(s) 148
MaeI CTAG 1 cut(s) 144
MaeII ACGT 1 cut(s) 23
MalI GATC 1 cut(s) 201
MboI GATC 1 cut(s) 199
MboII GAAGA 2 cut(s) 9, 277
MseI TTAA 1 cut(s) 204
NcoI CCATGG 1 cut(s) 224
NdeII GATC 1 cut(s) 199
NheI GCTAGC 1 cut(s) 143
NlaIII CATG 2 cut(s) 61, 228
NlaIV GGNNCC 1 cut(s) 222
PfeI GAWTC 1 cut(s) 245
PkrI GCNGC 3 cut(s) 113, 135, 220
PsiI TTATAA 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 222
PstI CTGCAG 1 cut(s) 116
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 3 cut(s) 112, 134, 219
Sau3AI GATC 1 cut(s) 199
SetI ASST 3 cut(s) 26, 149, 191
SfaNI GCATC 1 cut(s) 148
SfcI CTRYAG 1 cut(s) 112
SgeI CNNG 8 cut(s) 70, 120, 156, 160, 237, 270, 274, 287
SsiI CCGC 1 cut(s) 218
SspI AATATT 1 cut(s) 214
SspMI CTAG 1 cut(s) 144
StyI CCWWGG 1 cut(s) 224
TaiI ACGT 1 cut(s) 26
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 1 cut(s) 245
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TscAI CASTG 2 cut(s) 121, 360
TseI GCWGC 2 cut(s) 111, 133
TspDTI ATGAA 2 cut(s) 181, 352
TspRI CASTG 2 cut(s) 121, 360
XspI CTAG 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.