FvH4_6g45620

Protein trichome birefringence-like 19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013701
Physical Location & Seq
Reverse (-)
34978463 .. 34981417
2955 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g45620.t1

Sequence Viewer

Length: 1413 bp
ATGAAGTTTCATGCCATTGATCTCCCCAATGTGAAATACACACAACCCTACAGCATCTACAATAAGGTTCTCCTTCTGGCCCTTACCTTAATTGTTCTCACTTCAATTCCTCTATATATGAACAAGAGCTCAACTACTTCACCATTGACATCCCCAACACCATCTTTAACAACCAAAACCAGTAATGCTACTGGTTTGGAAAGCATATTGCCATCGCCAACACCATCCCCAACAATAGAAATCAATAGTACTGCTGGTTTGGAAAGCATATTGCCATCCCCAACATCATCCCCAACAACAGAAACCAATAGTACTACTGGTTTGGAAAGCATAGAAGAGAAAGAGGAGGAGCAATGCCAGCTATTTAGTGGGAACTGGATTCCTTACCCTGATGGACCTGCTTACTACACAAATGAGACTTGCGAATGGATCATTCCTCAGCAGAATTGCATGAAGTTTGGGAGGCCGGATTCGGAATTCATGAAGTGGAGGTGGAAGCCTGATGGTTGTGAGTTACCCTTGTTTGATGCAGCTGAGTTCTTGGAGATCTTTCGAGGGAAGTCCTTGGCTTTTATTGGGGACTCTGTGGGAAGAAACCAAATGCAGTCACTGTTGTGCATCTTAGGCAATGTGACTTATCCTGAGGACATTTCTGACCAGTACTCAAATAACACAGATTACTTCAAGCACTATGTCTACACTGATTACAATTTTACTATGGTAACTCTATGGGCTCCTTACTTGATTAGATATAGTTATGCAGACCCTAGTGTACATTCTGGGGACAGCCTCATGAATCTCTACTTAGACGAGCCAGATGAAGCGTGGACAAGTCAGGTTGAAAATTTCGACTATGTGATCTTCTCAGCAGGGCAATGGTTCTTAAGGCCGCTAATCTTTTACGAAAATGGTGGAAGTATTGGGTGTCTCTGGTGCAAACTGGACAACATGACAAAGATGTCAGAGTCCTATGGGTACAGAAAGGCCTTCAGGACCATGTTTAGGACGCTGCAGAGCCTCGAAAACTATAAAGGTGTGACATTTTTGAGGACTTTCTCTCCGGCACACTTTGAAAATGGGGATTGGAATGCTGGAGGGAATTGTGGGAGGACAAAGCCTTTTAGCAAAGAGGAAATGAAGTTAGAAGGATATAATTTGGACATGTATACGGCTCAAGTAGAGGAGTTCAAAGCAGCACAAGTCCAAGCCCAGCAGAAAGGTCTGGACTTTAGATTGATGGAAACAACTGAAGCAATGCTGCTCCGACCTGATGCACACCCGAATTACTATGGAGGATCACACCACAGGAACACGACTACTGCCGACTGTGTTCACTGGTGCTTGCCTGGCCCTATTGATACATGGAATGAGATTTTGCTGCATATGTTGAAGACACGGCAGAATTCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

471

Amino Acids

53.56

Weight (kDa)

4.97

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 117 - 171 3.2e-19 PMR5 N terminal Domain
PC-Esterase PF13839 172 - 462 5.7e-93 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013701)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45620
malus_domestica MD17G1072300.v1.1
prunus_persica Prupe.3G242400_v2.0.a1 Prupe.3G242900_v2.0.a1 Prupe.8G194900_v2.0.a1
pyrus_communis pycom09g00780 pycom17g07180 pycom17g07330
rosa_chinensis RchiOBHm_Chr2g0163771
rosa_laevigata RLG00000021414
rosa_multiflora Rmu_sc0004918.1_g000002
rosa_roxburghii Rroxscaffold_2G00087060
rosa_rugosa Rorug02G0504500
rosa_samantha Rh2AG572400 Rh2BG583000 Rh2CG553900 Rh2DG592700
rosa_wichuraiana Rw2G047310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 958
Acc36I ACCTGC 1 cut(s) 406
AccI GTMKAC 2 cut(s) 696, 1166
AciI CCGC 1 cut(s) 890
AclWI GGATC 2 cut(s) 437, 1303
AcsI RAATTY 3 cut(s) 476, 844, 1402
AcuI CTGAAG 2 cut(s) 973, 1269
AfaI GTAC 5 cut(s) 250, 313, 662, 774, 977
AfiI CCNNNNNNNGG 1 cut(s) 1002
AflII CTTAAG 1 cut(s) 883
AflIII ACRYGT 1 cut(s) 1161
AgsI TTSAA 7 cut(s) 105, 685, 842, 1073, 1189, 1390, 1407
AjnI CCWGG 1 cut(s) 1345
AleI CACNNNNGTG 1 cut(s) 613
AluBI AGCT 3 cut(s) 129, 361, 533
AluI AGCT 3 cut(s) 129, 361, 533
Alw21I GWGCWC 1 cut(s) 131
Alw26I GTCTC 2 cut(s) 410, 932
AlwI GGATC 2 cut(s) 437, 1303
AlwNI CAGNNNCTG 1 cut(s) 610
AoxI GGCC 5 cut(s) 78, 464, 887, 984, 1348
ApeKI GCWGC 5 cut(s) 530, 1009, 1193, 1258, 1378
ApoI RAATTY 3 cut(s) 476, 844, 1402
AspS9I GGNCC 4 cut(s) 79, 395, 993, 1349
AsuHPI GGTGA 1 cut(s) 132
AvaII GGWCC 2 cut(s) 395, 993
AxyI CCTNAGG 1 cut(s) 642
BanII GRGCYC 2 cut(s) 131, 736
BbsI GAAGAC 1 cut(s) 1397
Bbv12I GWGCWC 1 cut(s) 131
BbvCI CCTCAGC 1 cut(s) 438
BbvI GCAGC 5 cut(s) 542, 996, 1205, 1245, 1365
BccI CCATC 7 cut(s) 169, 220, 232, 283, 386, 497, 1231
BceAI ACGGC 1 cut(s) 1185
BciT130I CCWGG 1 cut(s) 1347
BcoDI GTCTC 2 cut(s) 410, 932
BfaI CTAG 1 cut(s) 768
BfmI CTRYAG 2 cut(s) 49, 1010
BfrI CTTAAG 1 cut(s) 883
BfuAI ACCTGC 1 cut(s) 406
BglII AGATCT 1 cut(s) 546
BisI GCNGC 6 cut(s) 531, 890, 1010, 1194, 1259, 1379
BlsI GCNGC 6 cut(s) 532, 891, 1011, 1195, 1260, 1380
BmcAI AGTACT 3 cut(s) 250, 313, 662
Bme1390I CCNGG 1 cut(s) 1347
Bme18I GGWCC 2 cut(s) 395, 993
BmgT120I GGNCC 4 cut(s) 79, 395, 993, 1349
BmiI GGNNCC 1 cut(s) 735
BmrFI CCNGG 1 cut(s) 1347
BmsI GCATC 4 cut(s) 63, 517, 627, 1261
BpiI GAAGAC 1 cut(s) 1397
BpmI CTGGAG 1 cut(s) 1113
Bpu10I CCTNAGC 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 1158
BsaJI CCNNGG 1 cut(s) 564
BsaXI ACNNNNNCTCC 2 cut(s) 1042, 1072
Bsc4I CCNNNNNNNGG 1 cut(s) 1002
Bse1I ACTGG 7 cut(s) 180, 196, 322, 380, 658, 945, 1340
Bse21I CCTNAGG 1 cut(s) 642
Bse3DI GCAATG 4 cut(s) 359, 634, 881, 1260
BseBI CCWGG 1 cut(s) 1347
BseDI CCNNGG 1 cut(s) 564
BseGI GGATG 4 cut(s) 149, 224, 275, 287
BseLI CCNNNNNNNGG 1 cut(s) 1002
BseMI GCAATG 4 cut(s) 359, 634, 881, 1260
BseMII CTCAG 4 cut(s) 452, 525, 633, 879
BseNI ACTGG 7 cut(s) 180, 196, 322, 380, 658, 945, 1340
BseRI GAGGAG 3 cut(s) 359, 362, 1196
BseXI GCAGC 5 cut(s) 542, 996, 1205, 1245, 1365
BseYI CCCAGC 1 cut(s) 1209
BshFI GGCC 5 cut(s) 80, 466, 889, 986, 1350
BsiHKAI GWGCWC 1 cut(s) 131
BsiSI CCGG 2 cut(s) 467, 1061
BslFI GGGAC 2 cut(s) 593, 797
BslI CCNNNNNNNGG 1 cut(s) 1002
BsmAI GTCTC 2 cut(s) 410, 932
BsmFI GGGAC 2 cut(s) 593, 797
BsmI GAATGC 1 cut(s) 1093
BsnI GGCC 5 cut(s) 80, 466, 889, 986, 1350
Bsp1286I GDGCHC 2 cut(s) 131, 736
Bsp1407I TGTACA 1 cut(s) 772
Bsp143I GATC 5 cut(s) 19, 429, 546, 858, 1295
BspACI CCGC 1 cut(s) 890
BspANI GGCC 5 cut(s) 80, 466, 889, 986, 1350
BspCNI CTCAG 4 cut(s) 451, 526, 634, 878
BspHI TCATGA 2 cut(s) 480, 792
BspLI GGNNCC 1 cut(s) 735
BspMAI CTGCAG 1 cut(s) 1014
BspMI ACCTGC 1 cut(s) 406
BspPI GGATC 2 cut(s) 437, 1303
BspTI CTTAAG 1 cut(s) 883
BsrDI GCAATG 4 cut(s) 359, 634, 881, 1260
BsrGI TGTACA 1 cut(s) 772
BsrI ACTGG 7 cut(s) 180, 196, 322, 380, 658, 945, 1340
BssECI CCNNGG 1 cut(s) 564
BssMI GATC 5 cut(s) 19, 429, 546, 858, 1295
BssNAI GTATAC 1 cut(s) 1167
BssT1I CCWWGG 1 cut(s) 564
Bst1107I GTATAC 1 cut(s) 1167
Bst2UI CCWGG 1 cut(s) 1347
Bst4CI ACNGT 2 cut(s) 612, 1328
Bst6I CTCTTC 1 cut(s) 330
BstAFI CTTAAG 1 cut(s) 883
BstAUI TGTACA 1 cut(s) 772
BstC8I GCNNGC 2 cut(s) 359, 1343
BstDEI CTNAG 6 cut(s) 438, 534, 622, 642, 805, 865
BstF5I GGATG 4 cut(s) 149, 224, 275, 287
BstKTI GATC 5 cut(s) 22, 432, 549, 861, 1298
BstMAI GTCTC 2 cut(s) 410, 932
BstMBI GATC 5 cut(s) 19, 429, 546, 858, 1295
BstMWI GCNNNNNNNGC 3 cut(s) 358, 624, 1347
BstNI CCWGG 1 cut(s) 1347
BstNSI RCATGY 1 cut(s) 1165
BstSCI CCNGG 1 cut(s) 1345
BstSFI CTRYAG 2 cut(s) 49, 1010
BstV1I GCAGC 5 cut(s) 542, 996, 1205, 1245, 1365
BstV2I GAAGAC 1 cut(s) 1397
BstX2I RGATCY 1 cut(s) 546
BstYI RGATCY 1 cut(s) 546
BstZ17I GTATAC 1 cut(s) 1167
Bsu36I CCTNAGG 1 cut(s) 642
BsuRI GGCC 5 cut(s) 80, 466, 889, 986, 1350
BtgZI GCGATG 1 cut(s) 198
BtsCI GGATG 4 cut(s) 149, 224, 275, 287
BtsIMutI CAGTG 3 cut(s) 608, 699, 1333
BveI ACCTGC 1 cut(s) 406
Cac8I GCNNGC 2 cut(s) 359, 1343
CaiI CAGNNNCTG 1 cut(s) 610
CciI TCATGA 2 cut(s) 480, 792
Cfr13I GGNCC 4 cut(s) 79, 395, 993, 1349
CseI GACGC 1 cut(s) 1015
Csp6I GTAC 5 cut(s) 249, 312, 661, 773, 976
CviAII CATG 8 cut(s) 11, 451, 481, 793, 949, 997, 1162, 1362
CviQI GTAC 5 cut(s) 249, 312, 661, 773, 976
DdeI CTNAG 6 cut(s) 438, 534, 622, 642, 805, 865
DpnI GATC 5 cut(s) 21, 431, 548, 860, 1297
DpnII GATC 5 cut(s) 19, 429, 546, 858, 1295
DrdI GACNNNNNNGTC 1 cut(s) 958
DseDI GACNNNNNNGTC 1 cut(s) 958
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Ecl136II GAGCTC 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 564
Eco147I AGGCCT 1 cut(s) 986
Eco24I GRGCYC 2 cut(s) 131, 736
Eco47I GGWCC 2 cut(s) 395, 993
Eco53kI GAGCTC 1 cut(s) 129
Eco57I CTGAAG 2 cut(s) 973, 1269
Eco81I CCTNAGG 1 cut(s) 642
EcoICRI GAGCTC 1 cut(s) 129
EcoRI GAATTC 2 cut(s) 476, 1402
EcoRII CCWGG 1 cut(s) 1345
EcoT14I CCWWGG 1 cut(s) 564
EcoT38I GRGCYC 2 cut(s) 131, 736
ErhI CCWWGG 1 cut(s) 564
FaeI CATG 8 cut(s) 14, 454, 484, 796, 952, 1000, 1165, 1365
FaqI GGGAC 2 cut(s) 593, 797
FatI CATG 8 cut(s) 10, 450, 480, 792, 948, 996, 1161, 1361
FauNDI CATATG 1 cut(s) 1383
FblI GTMKAC 2 cut(s) 696, 1166
Fnu4HI GCNGC 6 cut(s) 531, 890, 1010, 1194, 1259, 1379
FokI GGATG 4 cut(s) 136, 211, 262, 274
FriOI GRGCYC 2 cut(s) 131, 736
Fsp4HI GCNGC 6 cut(s) 531, 890, 1010, 1194, 1259, 1379
FspBI CTAG 1 cut(s) 768
GluI GCNGC 6 cut(s) 531, 890, 1010, 1194, 1259, 1379
GsaI CCCAGC 1 cut(s) 1213
GsuI CTGGAG 1 cut(s) 1113
HaeIII GGCC 5 cut(s) 80, 466, 889, 986, 1350
HapII CCGG 2 cut(s) 467, 1061
HgaI GACGC 1 cut(s) 1015
Hin1II CATG 8 cut(s) 14, 454, 484, 796, 952, 1000, 1165, 1365
HinfI GANTC 5 cut(s) 379, 470, 581, 796, 965
HpaII CCGG 2 cut(s) 467, 1061
HphI GGTGA 1 cut(s) 132
Hpy166II GTNNAC 5 cut(s) 697, 773, 828, 1167, 1333
Hpy188I TCNGA 4 cut(s) 475, 655, 964, 1265
Hpy188III TCNNGA 5 cut(s) 481, 641, 793, 991, 1223
Hpy8I GTNNAC 5 cut(s) 697, 773, 828, 1167, 1333
HpyAV CCTTC 3 cut(s) 83, 997, 1139
HpyCH4III ACNGT 2 cut(s) 612, 1328
HpyCH4V TGCA 9 cut(s) 450, 530, 604, 618, 761, 936, 1012, 1274, 1381
HpyF10VI GCNNNNNNNGC 3 cut(s) 358, 624, 1347
HpyF3I CTNAG 6 cut(s) 438, 534, 622, 642, 805, 865
Hsp92II CATG 8 cut(s) 14, 454, 484, 796, 952, 1000, 1165, 1365
Kzo9I GATC 5 cut(s) 19, 429, 546, 858, 1295
LmnI GCTCC 3 cut(s) 349, 739, 1266
Lsp1109I GCAGC 5 cut(s) 542, 996, 1205, 1245, 1365
LweI GCATC 4 cut(s) 63, 517, 627, 1261
MaeI CTAG 1 cut(s) 768
MaeIII GTNAC 5 cut(s) 513, 606, 631, 721, 1036
MalI GATC 5 cut(s) 21, 431, 548, 860, 1297
MboI GATC 5 cut(s) 19, 429, 546, 858, 1295
MboII GAAGA 4 cut(s) 347, 603, 853, 1402
MflI RGATCY 1 cut(s) 546
MhlI GDGCHC 2 cut(s) 131, 736
MlyI GAGTC 2 cut(s) 575, 974
MmeI TCCRAC 1 cut(s) 1288
MseI TTAA 3 cut(s) 89, 167, 884
MslI CAYNNNNRTG 1 cut(s) 613
MspA1I CMGCKG 1 cut(s) 533
MspCI CTTAAG 1 cut(s) 883
MspI CCGG 2 cut(s) 467, 1061
MspR9I CCNGG 1 cut(s) 1347
Mva1269I GAATGC 1 cut(s) 1093
MvaI CCWGG 1 cut(s) 1347
MwoI GCNNNNNNNGC 3 cut(s) 358, 624, 1347
NdeI CATATG 1 cut(s) 1383
NdeII GATC 5 cut(s) 19, 429, 546, 858, 1295
NlaIII CATG 8 cut(s) 14, 454, 484, 796, 952, 1000, 1165, 1365
NlaIV GGNNCC 1 cut(s) 735
NmuCI GTSAC 3 cut(s) 606, 631, 1036
NspI RCATGY 1 cut(s) 1165
OliI CACNNNNGTG 1 cut(s) 613
PagI TCATGA 2 cut(s) 480, 792
PceI AGGCCT 1 cut(s) 986
PciI ACATGT 1 cut(s) 1161
PctI GAATGC 1 cut(s) 1093
PfeI GAWTC 3 cut(s) 379, 470, 796
PkrI GCNGC 6 cut(s) 532, 891, 1011, 1195, 1260, 1380
PleI GAGTC 2 cut(s) 575, 973
PpsI GAGTC 2 cut(s) 575, 973
PscI ACATGT 1 cut(s) 1161
Psp124BI GAGCTC 1 cut(s) 131
Psp6I CCWGG 1 cut(s) 1345
PspFI CCCAGC 1 cut(s) 1209
PspGI CCWGG 1 cut(s) 1345
PspN4I GGNNCC 1 cut(s) 735
PspPI GGNCC 4 cut(s) 79, 395, 993, 1349
PstI CTGCAG 1 cut(s) 1014
PstNI CAGNNNCTG 1 cut(s) 610
PsuI RGATCY 1 cut(s) 546
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 5 cut(s) 250, 313, 662, 774, 977
RsaNI GTAC 5 cut(s) 249, 312, 661, 773, 976
RseI CAYNNNNRTG 1 cut(s) 613
SacI GAGCTC 1 cut(s) 131
SaqAI TTAA 3 cut(s) 89, 167, 884
SatI GCNGC 6 cut(s) 531, 890, 1010, 1194, 1259, 1379
Sau3AI GATC 5 cut(s) 19, 429, 546, 858, 1295
Sau96I GGNCC 4 cut(s) 79, 395, 993, 1349
ScaI AGTACT 3 cut(s) 250, 313, 662
SchI GAGTC 2 cut(s) 575, 974
ScrFI CCNGG 1 cut(s) 1347
SduI GDGCHC 2 cut(s) 131, 736
SfaNI GCATC 4 cut(s) 63, 517, 627, 1261
SfcI CTRYAG 2 cut(s) 49, 1010
SinI GGWCC 2 cut(s) 395, 993
SmiMI CAYNNNNRTG 1 cut(s) 613
SmlI CTYRAG 2 cut(s) 883, 1173
SmoI CTYRAG 2 cut(s) 883, 1173
SseBI AGGCCT 1 cut(s) 986
SsiI CCGC 1 cut(s) 890
SspMI CTAG 1 cut(s) 768
SstI GAGCTC 1 cut(s) 131
StuI AGGCCT 1 cut(s) 986
StyD4I CCNGG 1 cut(s) 1345
StyI CCWWGG 1 cut(s) 564
TaaI ACNGT 2 cut(s) 612, 1328
TaqI TCGA 3 cut(s) 553, 849, 1020
TatI WGTACW 4 cut(s) 248, 311, 660, 772
TauI GCSGC 1 cut(s) 892
TfiI GAWTC 3 cut(s) 379, 470, 796
Tru1I TTAA 3 cut(s) 89, 167, 884
Tru9I TTAA 3 cut(s) 89, 167, 884
TscAI CASTG 3 cut(s) 615, 706, 1340
TseFI GTSAC 3 cut(s) 606, 631, 1036
TseI GCWGC 5 cut(s) 530, 1009, 1193, 1258, 1378
Tsp45I GTSAC 3 cut(s) 606, 631, 1036
TspDTI ATGAA 8 cut(s) 17, 134, 467, 469, 497, 809, 834, 1151
TspRI CASTG 3 cut(s) 615, 706, 1340
Vha464I CTTAAG 1 cut(s) 883
VpaK11BI GGWCC 2 cut(s) 395, 993
XapI RAATTY 3 cut(s) 476, 844, 1402
XceI RCATGY 1 cut(s) 1165
XcmI CCANNNNNNNNNTGG 2 cut(s) 365, 822
XmiI GTMKAC 2 cut(s) 696, 1166
XspI CTAG 1 cut(s) 768
ZrmI AGTACT 3 cut(s) 250, 313, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.