MD17G1072300.v1.1

Protein trichome birefringence-like 19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013701
Physical Location & Seq
Forward (+)
5810776 .. 5812179
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1072300.v1.1.491

Sequence Viewer

Length: 1260 bp
ATGAAGTTTCAAGCCATTGGTCTGCCTAATGCAAAATACACACAACCAAACATCACCTACAAAAAAGGTATTCTTCTAGCCCTCACCTTACTTCTACTCACCACAATCCCTCTCTGTATAAACAACAGCACAACTTCTCCATTGCCATCTCCTAACAGCAACATTACCAGTGGATTAAAAACCCTACAAGATGAAGACCAATGCCAAATATTTAGTGGGAGTTGGATTCCATATCCAGATGGACCTGCTTACTACACAAATGAAACTTGCAATTTGATCCTTGATCAGCAGAACTGCTTGAAGTTCGGGAGGCCCGACAAGGAGTTCATGAAGTGGAGGTGGAAGCCCCAAGATTGCGAGTTGCCATTGTTCGATGCAGCTCAGTTCTTGGAGCTTGTTAGAGGGAAGTCATTAGCATTTCTTGGTGATTCTGTTGGAAGGAATCAAATGCAGTCCTTGCTGTGCCTTTTGTCCAATGTGACTTATCCTGAGGATGTTTCTCACAAGTATTCAACAAACACAGATTACTTCAAGCGCTACGTATACCATGACTACAACTTCACTATGGCAACTCTCTGGGCACCCTACTTGGTCAAATCTAGAGATGCCGACCCGAATGGCCACGGCATCAACAGCCTCATGAGCCTCTACTTAGACGAGCCCGACGAGGGATGGGAAACTGAGGTTGAAAATTTCGATTATGTCATCGTCTCAGCAGGGCAATGGTTCTTCCGGCCGCTGATATACTACGAAAATGGTCGCGTCATAGGGTGTCACAAGTGCGGACGAGACGACATGAAATCTTTTCTCACGTACTACGGTTACAAAAAGGCATTCCGAACAGTGTTTAGGACCCTTCGGAATCTCAAAAACTACAAAGGGGTGACGTTTTTGAGGACGTTTTCTCCGTCACACTTCGAAAACGGGGCGTGGAACGAGGGAGGGAATTGTCCGAGGACGAGGCCTTTCAGCAAGGAAGAAATGAAGCTAGATGGGTACATTTTGGAGATGCATTTGACACAAGTAGAGGAGCTAAAAGCAGCAGAAAAGAAAGGTATGAAAAGAGGCCTTGAATTTAGGTTAATGGATACAACTGAAGCTATGTTGCTGAGGCCTGATGGACATCCAAACTTCTACGGACAGTCACCTCACCGGAACATGACTATTGCTGACTGTGTCCACTGGTGCTTGCCTGGCCCCATTGATACGTGGAATGAGATTTTGCTGTATATGTTGAAATCCGGGCACAAACCATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

420

Amino Acids

48.32

Weight (kDa)

7.11

Isoelectric Point (pI)

38.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 67 - 120 4.9e-19 PMR5 N terminal Domain
PC-Esterase PF13839 121 - 410 4.6e-93 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013701)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45620
malus_domestica MD17G1072300.v1.1
prunus_persica Prupe.3G242400_v2.0.a1 Prupe.3G242900_v2.0.a1 Prupe.8G194900_v2.0.a1
pyrus_communis pycom09g00780 pycom17g07180 pycom17g07330
rosa_chinensis RchiOBHm_Chr2g0163771
rosa_laevigata RLG00000021414
rosa_multiflora Rmu_sc0004918.1_g000002
rosa_roxburghii Rroxscaffold_2G00087060
rosa_rugosa Rorug02G0504500
rosa_samantha Rh2AG572400 Rh2BG583000 Rh2CG553900 Rh2DG592700
rosa_wichuraiana Rw2G047310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 253
AccB1I GGYRCC 1 cut(s) 580
AccI GTMKAC 1 cut(s) 543
AccII CGCG 1 cut(s) 762
AciI CCGC 2 cut(s) 737, 783
AclWI GGATC 1 cut(s) 271
AcoI YGGCCR 2 cut(s) 619, 734
AcsI RAATTY 2 cut(s) 691, 1073
AcuI CTGAAG 1 cut(s) 1116
AfaI GTAC 2 cut(s) 815, 998
AfeI AGCGCT 1 cut(s) 536
AfiI CCNNNNNNNGG 1 cut(s) 668
AgsI TTSAA 7 cut(s) 11, 301, 513, 532, 689, 1073, 1237
AjnI CCWGG 1 cut(s) 1192
AluBI AGCT 5 cut(s) 380, 394, 988, 1033, 1100
AluI AGCT 5 cut(s) 380, 394, 988, 1033, 1100
Alw26I GTCTC 2 cut(s) 715, 783
AlwI GGATC 1 cut(s) 271
Aor51HI AGCGCT 1 cut(s) 536
AoxI GGCC 7 cut(s) 311, 619, 734, 962, 1066, 1112, 1195
ApeKI GCWGC 2 cut(s) 377, 1040
ApoI RAATTY 2 cut(s) 691, 1073
AspLEI GCGC 1 cut(s) 537
AspS9I GGNCC 4 cut(s) 242, 312, 852, 1196
AsuC2I CCSGG 1 cut(s) 1243
AsuHPI GGTGA 7 cut(s) 46, 76, 91, 437, 895, 1137, 1142
AsuII TTCGAA 1 cut(s) 918
AvaII GGWCC 2 cut(s) 242, 852
AxyI CCTNAGG 1 cut(s) 489
BaeGI GKGCMC 2 cut(s) 583, 1248
BalI TGGCCA 1 cut(s) 621
BanI GGYRCC 1 cut(s) 580
BanII GRGCYC 1 cut(s) 663
BbsI GAAGAC 1 cut(s) 201
BbvCI CCTCAGC 1 cut(s) 1109
BbvI GCAGC 2 cut(s) 389, 1052
BccI CCATC 5 cut(s) 154, 233, 666, 986, 1112
BceAI ACGGC 1 cut(s) 640
BciT130I CCWGG 1 cut(s) 1194
BciVI GTATCC 1 cut(s) 1081
BclI TGATCA 1 cut(s) 283
BcnI CCSGG 1 cut(s) 1243
BcoDI GTCTC 2 cut(s) 715, 783
BfaI CTAG 3 cut(s) 77, 600, 989
BfoI RGCGCY 1 cut(s) 538
BfuAI ACCTGC 1 cut(s) 253
BfuI GTATCC 1 cut(s) 1081
BisI GCNGC 3 cut(s) 378, 737, 1041
BlsI GCNGC 3 cut(s) 379, 738, 1042
Bme1390I CCNGG 2 cut(s) 1194, 1243
Bme18I GGWCC 2 cut(s) 242, 852
BmgT120I GGNCC 4 cut(s) 242, 312, 852, 1196
BmiI GGNNCC 3 cut(s) 582, 854, 1198
BmrFI CCNGG 2 cut(s) 1194, 1243
BmsI GCATC 4 cut(s) 364, 595, 636, 999
BpiI GAAGAC 1 cut(s) 201
Bpu10I CCTNAGC 1 cut(s) 1109
Bpu14I TTCGAA 1 cut(s) 918
BpuMI CCSGG 1 cut(s) 1243
BsaAI YACGTR 3 cut(s) 541, 813, 1209
BsaBI GATNNNNATC 1 cut(s) 1122
BsaJI CCNNGG 2 cut(s) 622, 953
BsaWI WCCGGW 1 cut(s) 1152
BsaXI ACNNNNNCTCC 4 cut(s) 121, 151, 889, 919
Bsc4I CCNNNNNNNGG 1 cut(s) 668
Bse1I ACTGG 2 cut(s) 168, 1187
Bse21I CCTNAGG 1 cut(s) 489
Bse3DI GCAATG 2 cut(s) 140, 728
Bse8I GATNNNNATC 1 cut(s) 1122
BseBI CCWGG 1 cut(s) 1194
BseDI CCNNGG 2 cut(s) 622, 953
BseGI GGATG 4 cut(s) 499, 677, 1123, 1253
BseJI GATNNNNATC 1 cut(s) 1122
BseLI CCNNNNNNNGG 1 cut(s) 668
BseMI GCAATG 2 cut(s) 140, 728
BseMII CTCAG 5 cut(s) 395, 480, 672, 726, 1100
BseNI ACTGG 2 cut(s) 168, 1187
BseRI GAGGAG 1 cut(s) 1043
BseSI GKGCMC 2 cut(s) 583, 1248
BseX3I CGGCCG 1 cut(s) 734
BseXI GCAGC 2 cut(s) 389, 1052
Bsh1236I CGCG 1 cut(s) 762
Bsh1285I CGRYCG 1 cut(s) 737
BshFI GGCC 7 cut(s) 313, 621, 736, 964, 1068, 1114, 1197
BshNI GGYRCC 1 cut(s) 580
BsiEI CGRYCG 1 cut(s) 737
BsiSI CCGG 3 cut(s) 733, 1153, 1242
BslI CCNNNNNNNGG 1 cut(s) 668
BsmAI GTCTC 2 cut(s) 715, 783
BsmBI CGTCTC 2 cut(s) 715, 783
BsmI GAATGC 1 cut(s) 833
BsnI GGCC 7 cut(s) 313, 621, 736, 964, 1068, 1114, 1197
Bsp119I TTCGAA 1 cut(s) 918
Bsp1286I GDGCHC 3 cut(s) 583, 663, 1248
Bsp143I GATC 2 cut(s) 276, 283
BspACI CCGC 2 cut(s) 737, 783
BspANI GGCC 7 cut(s) 313, 621, 736, 964, 1068, 1114, 1197
BspCNI CTCAG 5 cut(s) 394, 481, 673, 725, 1101
BspFNI CGCG 1 cut(s) 762
BspHI TCATGA 2 cut(s) 327, 639
BspLI GGNNCC 3 cut(s) 582, 854, 1198
BspMI ACCTGC 1 cut(s) 253
BspPI GGATC 1 cut(s) 271
BspT104I TTCGAA 1 cut(s) 918
BspT107I GGYRCC 1 cut(s) 580
BsrDI GCAATG 2 cut(s) 140, 728
BsrI ACTGG 2 cut(s) 168, 1187
BssECI CCNNGG 2 cut(s) 622, 953
BssMI GATC 2 cut(s) 276, 283
BssNAI GTATAC 1 cut(s) 544
Bst1107I GTATAC 1 cut(s) 544
Bst2UI CCWGG 1 cut(s) 1194
Bst4CI ACNGT 4 cut(s) 821, 844, 1143, 1175
BstAPI GCANNNNNTGC 1 cut(s) 457
BstBAI YACGTR 3 cut(s) 541, 813, 1209
BstBI TTCGAA 1 cut(s) 918
BstC8I GCNNGC 1 cut(s) 1190
BstDEI CTNAG 6 cut(s) 381, 489, 652, 681, 712, 1109
BstDSI CCRYGG 1 cut(s) 622
BstF5I GGATG 4 cut(s) 499, 677, 1123, 1253
BstFNI CGCG 1 cut(s) 762
BstH2I RGCGCY 1 cut(s) 538
BstHHI GCGC 1 cut(s) 537
BstKTI GATC 2 cut(s) 279, 286
BstMAI GTCTC 2 cut(s) 715, 783
BstMBI GATC 2 cut(s) 276, 283
BstMCI CGRYCG 1 cut(s) 737
BstMWI GCNNNNNNNGC 4 cut(s) 457, 633, 642, 1194
BstNI CCWGG 1 cut(s) 1194
BstSCI CCNGG 2 cut(s) 1192, 1241
BstSLI GKGCMC 2 cut(s) 583, 1248
BstSNI TACGTA 1 cut(s) 541
BstUI CGCG 1 cut(s) 762
BstV1I GCAGC 2 cut(s) 389, 1052
BstV2I GAAGAC 1 cut(s) 201
BstZ17I GTATAC 1 cut(s) 544
BstZI CGGCCG 1 cut(s) 734
Bsu36I CCTNAGG 1 cut(s) 489
BsuI GTATCC 1 cut(s) 1081
BsuRI GGCC 7 cut(s) 313, 621, 736, 964, 1068, 1114, 1197
BtgI CCRYGG 1 cut(s) 622
BtsCI GGATG 4 cut(s) 499, 677, 1123, 1253
BtsIMutI CAGTG 3 cut(s) 175, 849, 1180
BveI ACCTGC 1 cut(s) 253
Cac8I GCNNGC 1 cut(s) 1190
CciI TCATGA 2 cut(s) 327, 639
CfoI GCGC 1 cut(s) 537
Cfr13I GGNCC 4 cut(s) 242, 312, 852, 1196
CseI GACGC 1 cut(s) 751
Csp6I GTAC 2 cut(s) 814, 997
CviAII CATG 5 cut(s) 328, 548, 640, 796, 1159
CviQI GTAC 2 cut(s) 814, 997
DdeI CTNAG 6 cut(s) 381, 489, 652, 681, 712, 1109
DpnI GATC 2 cut(s) 278, 285
DpnII GATC 2 cut(s) 276, 283
EaeI YGGCCR 2 cut(s) 619, 734
EagI CGGCCG 1 cut(s) 734
EclXI CGGCCG 1 cut(s) 734
Eco105I TACGTA 1 cut(s) 541
Eco147I AGGCCT 3 cut(s) 964, 1068, 1114
Eco24I GRGCYC 1 cut(s) 663
Eco47I GGWCC 2 cut(s) 242, 852
Eco47III AGCGCT 1 cut(s) 536
Eco52I CGGCCG 1 cut(s) 734
Eco57I CTGAAG 1 cut(s) 1116
Eco81I CCTNAGG 1 cut(s) 489
EcoO109I RGGNCCY 1 cut(s) 852
EcoRII CCWGG 1 cut(s) 1192
EcoT22I ATGCAT 1 cut(s) 1014
EcoT38I GRGCYC 1 cut(s) 663
Esp3I CGTCTC 2 cut(s) 715, 783
FaeI CATG 5 cut(s) 331, 551, 643, 799, 1162
FalI AAGNNNNNCTT 2 cut(s) 57, 89
FatI CATG 5 cut(s) 327, 547, 639, 795, 1158
FbaI TGATCA 1 cut(s) 283
FblI GTMKAC 1 cut(s) 543
Fnu4HI GCNGC 3 cut(s) 378, 737, 1041
FokI GGATG 4 cut(s) 506, 684, 1110, 1240
FriOI GRGCYC 1 cut(s) 663
Fsp4HI GCNGC 3 cut(s) 378, 737, 1041
FspBI CTAG 3 cut(s) 77, 600, 989
GlaI GCGC 1 cut(s) 536
GluI GCNGC 3 cut(s) 378, 737, 1041
HaeII RGCGCY 1 cut(s) 538
HaeIII GGCC 7 cut(s) 313, 621, 736, 964, 1068, 1114, 1197
HapII CCGG 3 cut(s) 733, 1153, 1242
HgaI GACGC 1 cut(s) 751
HhaI GCGC 1 cut(s) 537
Hin1II CATG 5 cut(s) 331, 551, 643, 799, 1162
Hin6I GCGC 1 cut(s) 535
HinP1I GCGC 1 cut(s) 535
HinfI GANTC 4 cut(s) 226, 428, 442, 862
HpaII CCGG 3 cut(s) 733, 1153, 1242
HphI GGTGA 7 cut(s) 46, 76, 91, 437, 895, 1137, 1142
Hpy166II GTNNAC 2 cut(s) 544, 1180
Hpy188I TCNGA 3 cut(s) 839, 861, 954
Hpy188III TCNNGA 7 cut(s) 236, 307, 328, 488, 600, 640, 1257
Hpy8I GTNNAC 2 cut(s) 544, 1180
Hpy99I CGWCG 1 cut(s) 668
HpyAV CCTTC 2 cut(s) 432, 866
HpyCH4III ACNGT 4 cut(s) 821, 844, 1143, 1175
HpyCH4IV ACGT 5 cut(s) 540, 812, 887, 899, 1208
HpyCH4V TGCA 5 cut(s) 32, 270, 377, 451, 1012
HpyF10VI GCNNNNNNNGC 4 cut(s) 457, 633, 642, 1194
HpyF3I CTNAG 6 cut(s) 381, 489, 652, 681, 712, 1109
HpySE526I ACGT 5 cut(s) 540, 812, 887, 899, 1208
Hsp92II CATG 5 cut(s) 331, 551, 643, 799, 1162
HspAI GCGC 1 cut(s) 535
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 2 cut(s) 276, 283
LmnI GCTCC 2 cut(s) 391, 1030
Lsp1109I GCAGC 2 cut(s) 389, 1052
LweI GCATC 4 cut(s) 364, 595, 636, 999
MaeI CTAG 3 cut(s) 77, 600, 989
MaeII ACGT 5 cut(s) 540, 812, 887, 899, 1208
MaeIII GTNAC 6 cut(s) 478, 773, 821, 883, 909, 1143
MalI GATC 2 cut(s) 278, 285
MboI GATC 2 cut(s) 276, 283
MboII GAAGA 4 cut(s) 65, 206, 721, 989
MhlI GDGCHC 3 cut(s) 583, 663, 1248
MlsI TGGCCA 1 cut(s) 621
MluCI AATT 4 cut(s) 271, 691, 946, 1073
MluNI TGGCCA 1 cut(s) 621
MmeI TCCRAC 2 cut(s) 203, 415
Mox20I TGGCCA 1 cut(s) 621
Mph1103I ATGCAT 1 cut(s) 1014
MscI TGGCCA 1 cut(s) 621
MseI TTAA 2 cut(s) 176, 1082
Msp20I TGGCCA 1 cut(s) 621
MspA1I CMGCKG 1 cut(s) 739
MspI CCGG 3 cut(s) 733, 1153, 1242
MspR9I CCNGG 2 cut(s) 1194, 1243
Mva1269I GAATGC 1 cut(s) 833
MvaI CCWGG 1 cut(s) 1194
MvnI CGCG 1 cut(s) 762
MwoI GCNNNNNNNGC 4 cut(s) 457, 633, 642, 1194
NciI CCSGG 1 cut(s) 1243
NdeII GATC 2 cut(s) 276, 283
NlaIII CATG 5 cut(s) 331, 551, 643, 799, 1162
NlaIV GGNNCC 3 cut(s) 582, 854, 1198
NmuCI GTSAC 5 cut(s) 478, 773, 883, 909, 1143
NsiI ATGCAT 1 cut(s) 1014
NspV TTCGAA 1 cut(s) 918
PagI TCATGA 2 cut(s) 327, 639
PceI AGGCCT 3 cut(s) 964, 1068, 1114
PcsI WCGNNNNNNNCGW 3 cut(s) 312, 663, 905
PctI GAATGC 1 cut(s) 833
PfeI GAWTC 4 cut(s) 226, 428, 442, 862
PflFI GACNNNGTC 1 cut(s) 1175
PkrI GCNGC 3 cut(s) 379, 738, 1042
Ppu21I YACGTR 3 cut(s) 541, 813, 1209
PpuMI RGGWCCY 1 cut(s) 852
Psp5II RGGWCCY 1 cut(s) 852
Psp6I CCWGG 1 cut(s) 1192
PspGI CCWGG 1 cut(s) 1192
PspN4I GGNNCC 3 cut(s) 582, 854, 1198
PspPI GGNCC 4 cut(s) 242, 312, 852, 1196
PspPPI RGGWCCY 1 cut(s) 852
PsyI GACNNNGTC 1 cut(s) 1175
RsaI GTAC 2 cut(s) 815, 998
RsaNI GTAC 2 cut(s) 814, 997
SaqAI TTAA 2 cut(s) 176, 1082
SatI GCNGC 3 cut(s) 378, 737, 1041
Sau3AI GATC 2 cut(s) 276, 283
Sau96I GGNCC 4 cut(s) 242, 312, 852, 1196
ScrFI CCNGG 2 cut(s) 1194, 1243
SduI GDGCHC 3 cut(s) 583, 663, 1248
SfaNI GCATC 4 cut(s) 364, 595, 636, 999
SfuI TTCGAA 1 cut(s) 918
SinI GGWCC 2 cut(s) 242, 852
SnaBI TACGTA 1 cut(s) 541
Sse9I AATT 4 cut(s) 271, 691, 946, 1073
SseBI AGGCCT 3 cut(s) 964, 1068, 1114
SsiI CCGC 2 cut(s) 737, 783
SspI AATATT 1 cut(s) 210
SspMI CTAG 3 cut(s) 77, 600, 989
StuI AGGCCT 3 cut(s) 964, 1068, 1114
StyD4I CCNGG 2 cut(s) 1192, 1241
TaaI ACNGT 4 cut(s) 821, 844, 1143, 1175
TaiI ACGT 5 cut(s) 543, 815, 890, 902, 1211
TaqI TCGA 3 cut(s) 372, 696, 918
TasI AATT 4 cut(s) 271, 691, 946, 1073
TauI GCSGC 1 cut(s) 739
TfiI GAWTC 4 cut(s) 226, 428, 442, 862
Tru1I TTAA 2 cut(s) 176, 1082
Tru9I TTAA 2 cut(s) 176, 1082
TscAI CASTG 3 cut(s) 175, 849, 1187
TseFI GTSAC 5 cut(s) 478, 773, 883, 909, 1143
TseI GCWGC 2 cut(s) 377, 1040
Tsp45I GTSAC 5 cut(s) 478, 773, 883, 909, 1143
TspDTI ATGAA 8 cut(s) 17, 207, 276, 316, 344, 812, 998, 1073
TspGWI ACGGA 2 cut(s) 897, 1152
TspRI CASTG 3 cut(s) 175, 849, 1187
Tth111I GACNNNGTC 1 cut(s) 1175
VpaK11BI GGWCC 2 cut(s) 242, 852
XapI RAATTY 2 cut(s) 691, 1073
XbaI TCTAGA 1 cut(s) 599
XcmI CCANNNNNNNNNTGG 1 cut(s) 212
XmiI GTMKAC 1 cut(s) 543
XspI CTAG 3 cut(s) 77, 600, 989
Zsp2I ATGCAT 1 cut(s) 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.