Rroxscaffold_2G00087060

Protein trichome birefringence-like 19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013701
Physical Location & Seq
Forward (+)
9174953 .. 9176476
1524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00087060.1

Sequence Viewer

Length: 1275 bp
ATGAAGTTTCATGCCATTGATCTCCCCAGTGTGAAATACACACAACCCTACAGCATCTACAGAAGGGTTCTCCTTCTGGCCCTTACCTTAATTGTTCTCACTTCAATTCCTCTCTATGTGAACAAGAGCTCAACTACTTCACCATTGCCATCCCCAACAACCAAAACCAGTACTACTACTGGTTTGGAAAGCATAGAAGAGAAAGAGGAGGAGCAATGCCATATATTTAGTGGGAACTGGGTTCCTTACCCTGATGGACCTGCTTACTACACTAATGAGACTTGCAAATTGATCATTCCTCAGCAGAATTGCATGAAGTTTGGGAGACCGGATTCAGAGTTCATGAAGTGGAGGTGGAAGCCAGATGGTTGTGAGTTACCTTTGTTTGATGCAGCCGAGTTTTTGGAGATCTTTAGAGGGAAGTCCTTGGCTTTTCTTGGGGACTCTGTGGGAAGGAATCAAATGCAGTCATTGTTGTGCCTCTTAGCCAATGTGACTTATCCTGAGGACCTTTCTGACCAGTACTCAAATGACACAGATTACTTCAAGCACTATGTCTACACTGATTACAATTTTACCATGGCAACTCTATGGGCTCCTTACTTGGTTAGATCTAGTGATGCAGACCCTAGTGGACATTCCCGGAACAGCCTCATGAACCTCTACTTAGATGAGCCAGACGAGGCCTGGACAAGTCAGGTTGAAAATTTCGACTATGTGATCGTCTCAGCAGGGCAATGGTTCTTCCGGCCGCTAGTGTATTATGAAAATGGTGGAAGTATTGGGTGTCACTGGTGCAACCTAGACAACATGACAGAGATTCCAGAGACCTATGGGTACAGAAAGGCCTTCAGGACCGTGTTTAGGACACTCCAGAACATCAAAAACTATAAGGGTGTGACATTTTTGAGGACTTTCTCTCCAGCACACTTTGAAAATGGGGCTTGGAATGCTGGAGGGAGTTGTGGGAGGACAAAGCCTTTTAGCAAAGAGGAAATGAAGTTAGAAGGATTTATTTTGGACATGTATATGACCCAAGTAGAAGAGCTCAAAGCAGCACAAAATCAAGCTAAGCAGAAAGGTCTGGACTTTAGATTGATGGAAACAACTGAAGCAATGCTGCTGAGACCTGATGGACACCCAAATTTCTATGGAGCCTCACCGCACAGGAATACGACTTATGCCGACTGTGTTCACTGGTGCTTGCCCGGCCCTATTGATACATGGAATGAGATTTTGCTGCATATGTTGAAGACAGGGAAGAGATCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

424

Amino Acids

48.71

Weight (kDa)

5.64

Isoelectric Point (pI)

44.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 71 - 125 4.5e-20 PMR5 N terminal Domain
PC-Esterase PF13839 126 - 416 4.5e-93 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013701)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45620
malus_domestica MD17G1072300.v1.1
prunus_persica Prupe.3G242400_v2.0.a1 Prupe.3G242900_v2.0.a1 Prupe.8G194900_v2.0.a1
pyrus_communis pycom09g00780 pycom17g07180 pycom17g07330
rosa_chinensis RchiOBHm_Chr2g0163771
rosa_laevigata RLG00000021414
rosa_multiflora Rmu_sc0004918.1_g000002
rosa_roxburghii Rroxscaffold_2G00087060
rosa_rugosa Rorug02G0504500
rosa_samantha Rh2AG572400 Rh2BG583000 Rh2CG553900 Rh2DG592700
rosa_wichuraiana Rw2G047310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 268
AccI GTMKAC 1 cut(s) 558
AciI CCGC 2 cut(s) 752, 1163
AcoI YGGCCR 1 cut(s) 749
AcsI RAATTY 2 cut(s) 706, 1144
AcuI CTGAAG 2 cut(s) 835, 1131
AfaI GTAC 3 cut(s) 172, 524, 839
AfiI CCNNNNNNNGG 1 cut(s) 864
AflIII ACRYGT 1 cut(s) 1023
AgsI TTSAA 5 cut(s) 105, 547, 704, 935, 1252
AjnI CCWGG 1 cut(s) 686
AloI GAACNNNNNNTCC 2 cut(s) 323, 355
AluBI AGCT 3 cut(s) 129, 1048, 1070
AluI AGCT 3 cut(s) 129, 1048, 1070
Alw21I GWGCWC 2 cut(s) 131, 1050
Alw26I GTCTC 5 cut(s) 272, 319, 730, 821, 1120
AoxI GGCC 5 cut(s) 78, 684, 749, 846, 1210
ApeKI GCWGC 4 cut(s) 392, 1055, 1120, 1240
ApoI RAATTY 2 cut(s) 706, 1144
AspS9I GGNCC 5 cut(s) 79, 257, 508, 855, 1211
AsuC2I CCSGG 2 cut(s) 643, 1209
AsuHPI GGTGA 2 cut(s) 132, 1152
AvaII GGWCC 3 cut(s) 257, 508, 855
AxyI CCTNAGG 1 cut(s) 504
BanII GRGCYC 3 cut(s) 131, 598, 1050
BbsI GAAGAC 1 cut(s) 1259
Bbv12I GWGCWC 2 cut(s) 131, 1050
BbvCI CCTCAGC 1 cut(s) 300
BbvI GCAGC 4 cut(s) 404, 1067, 1107, 1227
BccI CCATC 5 cut(s) 157, 248, 359, 1093, 1127
BciT130I CCWGG 1 cut(s) 688
BclI TGATCA 1 cut(s) 291
BcnI CCSGG 2 cut(s) 643, 1209
BcoDI GTCTC 5 cut(s) 272, 319, 730, 821, 1120
BfaI CTAG 4 cut(s) 615, 630, 755, 803
BfmI CTRYAG 2 cut(s) 49, 58
BfuAI ACCTGC 1 cut(s) 268
BglII AGATCT 2 cut(s) 408, 611
BisI GCNGC 5 cut(s) 393, 752, 1056, 1121, 1241
BlpI GCTNAGC 1 cut(s) 1071
BlsI GCNGC 5 cut(s) 394, 753, 1057, 1122, 1242
BmcAI AGTACT 2 cut(s) 172, 524
Bme1390I CCNGG 3 cut(s) 643, 688, 1209
Bme18I GGWCC 3 cut(s) 257, 508, 855
BmgT120I GGNCC 5 cut(s) 79, 257, 508, 855, 1211
BmiI GGNNCC 3 cut(s) 243, 597, 1156
BmrFI CCNGG 3 cut(s) 643, 688, 1209
BmrI ACTGGG 2 cut(s) 21, 247
BmsI GCATC 3 cut(s) 63, 379, 610
BmuI ACTGGG 2 cut(s) 21, 247
BpiI GAAGAC 1 cut(s) 1259
BpmI CTGGAG 3 cut(s) 857, 906, 975
Bpu10I CCTNAGC 1 cut(s) 300
Bpu1102I GCTNAGC 1 cut(s) 1071
BpuMI CCSGG 2 cut(s) 643, 1209
BsaI GGTCTC 3 cut(s) 319, 821, 1120
BsaJI CCNNGG 2 cut(s) 426, 579
BsaWI WCCGGW 1 cut(s) 328
BsaXI ACNNNNNCTCC 2 cut(s) 904, 934
Bsc4I CCNNNNNNNGG 1 cut(s) 864
Bse1I ACTGG 7 cut(s) 27, 168, 184, 242, 520, 797, 1202
Bse21I CCTNAGG 1 cut(s) 504
Bse3DI GCAATG 4 cut(s) 143, 221, 743, 1122
BseBI CCWGG 1 cut(s) 688
BseDI CCNNGG 2 cut(s) 426, 579
BseGI GGATG 1 cut(s) 149
BseLI CCNNNNNNNGG 1 cut(s) 864
BseMI GCAATG 4 cut(s) 143, 221, 743, 1122
BseMII CTCAG 4 cut(s) 314, 495, 741, 1115
BseNI ACTGG 7 cut(s) 27, 168, 184, 242, 520, 797, 1202
BseRI GAGGAG 2 cut(s) 221, 224
BseX3I CGGCCG 1 cut(s) 749
BseXI GCAGC 4 cut(s) 404, 1067, 1107, 1227
Bsh1285I CGRYCG 1 cut(s) 752
BshFI GGCC 5 cut(s) 80, 686, 751, 848, 1212
BsiEI CGRYCG 1 cut(s) 752
BsiHKAI GWGCWC 2 cut(s) 131, 1050
BsiSI CCGG 4 cut(s) 329, 643, 748, 1209
BslFI GGGAC 1 cut(s) 455
BslI CCNNNNNNNGG 1 cut(s) 864
BsmAI GTCTC 5 cut(s) 272, 319, 730, 821, 1120
BsmBI CGTCTC 1 cut(s) 730
BsmFI GGGAC 1 cut(s) 455
BsmI GAATGC 1 cut(s) 955
BsnI GGCC 5 cut(s) 80, 686, 751, 848, 1212
Bso31I GGTCTC 3 cut(s) 319, 821, 1120
Bsp1286I GDGCHC 3 cut(s) 131, 598, 1050
Bsp143I GATC 6 cut(s) 19, 291, 408, 611, 720, 1265
Bsp1720I GCTNAGC 1 cut(s) 1071
Bsp19I CCATGG 1 cut(s) 579
BspACI CCGC 2 cut(s) 752, 1163
BspANI GGCC 5 cut(s) 80, 686, 751, 848, 1212
BspCNI CTCAG 4 cut(s) 313, 496, 740, 1116
BspHI TCATGA 2 cut(s) 342, 654
BspLI GGNNCC 3 cut(s) 243, 597, 1156
BspMI ACCTGC 1 cut(s) 268
BspQI GCTCTTC 1 cut(s) 1038
BspTNI GGTCTC 3 cut(s) 319, 821, 1120
BsrDI GCAATG 4 cut(s) 143, 221, 743, 1122
BsrI ACTGG 7 cut(s) 27, 168, 184, 242, 520, 797, 1202
BssECI CCNNGG 2 cut(s) 426, 579
BssMI GATC 6 cut(s) 19, 291, 408, 611, 720, 1265
BssT1I CCWWGG 2 cut(s) 426, 579
Bst2UI CCWGG 1 cut(s) 688
Bst4CI ACNGT 2 cut(s) 859, 1190
Bst6I CTCTTC 3 cut(s) 192, 1038, 1256
BstC8I GCNNGC 1 cut(s) 1205
BstDEI CTNAG 7 cut(s) 300, 484, 504, 667, 727, 1071, 1124
BstDSI CCRYGG 1 cut(s) 579
BstF5I GGATG 1 cut(s) 149
BstKTI GATC 6 cut(s) 22, 294, 411, 614, 723, 1268
BstMAI GTCTC 5 cut(s) 272, 319, 730, 821, 1120
BstMBI GATC 6 cut(s) 19, 291, 408, 611, 720, 1265
BstMCI CGRYCG 1 cut(s) 752
BstMWI GCNNNNNNNGC 2 cut(s) 950, 1209
BstNI CCWGG 1 cut(s) 688
BstNSI RCATGY 1 cut(s) 1027
BstSCI CCNGG 3 cut(s) 641, 686, 1207
BstSFI CTRYAG 2 cut(s) 49, 58
BstV1I GCAGC 4 cut(s) 404, 1067, 1107, 1227
BstV2I GAAGAC 1 cut(s) 1259
BstX2I RGATCY 2 cut(s) 408, 611
BstYI RGATCY 2 cut(s) 408, 611
BstZI CGGCCG 1 cut(s) 749
Bsu36I CCTNAGG 1 cut(s) 504
BsuRI GGCC 5 cut(s) 80, 686, 751, 848, 1212
BtgI CCRYGG 1 cut(s) 579
BtsCI GGATG 1 cut(s) 149
BtsIMutI CAGTG 4 cut(s) 34, 561, 790, 1195
BveI ACCTGC 1 cut(s) 268
Cac8I GCNNGC 1 cut(s) 1205
CciI TCATGA 2 cut(s) 342, 654
Cfr13I GGNCC 5 cut(s) 79, 257, 508, 855, 1211
Csp6I GTAC 3 cut(s) 171, 523, 838
CviAII CATG 8 cut(s) 11, 313, 343, 580, 655, 811, 1024, 1224
CviQI GTAC 3 cut(s) 171, 523, 838
DdeI CTNAG 7 cut(s) 300, 484, 504, 667, 727, 1071, 1124
DpnI GATC 6 cut(s) 21, 293, 410, 613, 722, 1267
DpnII GATC 6 cut(s) 19, 291, 408, 611, 720, 1265
EaeI YGGCCR 1 cut(s) 749
EagI CGGCCG 1 cut(s) 749
Eam1104I CTCTTC 3 cut(s) 192, 1038, 1256
EarI CTCTTC 3 cut(s) 192, 1038, 1256
Ecl136II GAGCTC 2 cut(s) 129, 1048
EclXI CGGCCG 1 cut(s) 749
Eco130I CCWWGG 2 cut(s) 426, 579
Eco147I AGGCCT 2 cut(s) 686, 848
Eco24I GRGCYC 3 cut(s) 131, 598, 1050
Eco31I GGTCTC 3 cut(s) 319, 821, 1120
Eco47I GGWCC 3 cut(s) 257, 508, 855
Eco52I CGGCCG 1 cut(s) 749
Eco53kI GAGCTC 2 cut(s) 129, 1048
Eco57I CTGAAG 2 cut(s) 835, 1131
Eco81I CCTNAGG 1 cut(s) 504
EcoICRI GAGCTC 2 cut(s) 129, 1048
EcoO109I RGGNCCY 1 cut(s) 508
EcoRII CCWGG 1 cut(s) 686
EcoT14I CCWWGG 2 cut(s) 426, 579
EcoT38I GRGCYC 3 cut(s) 131, 598, 1050
ErhI CCWWGG 2 cut(s) 426, 579
Esp3I CGTCTC 1 cut(s) 730
FaeI CATG 8 cut(s) 14, 316, 346, 583, 658, 814, 1027, 1227
FaqI GGGAC 1 cut(s) 455
FatI CATG 8 cut(s) 10, 312, 342, 579, 654, 810, 1023, 1223
FauNDI CATATG 1 cut(s) 1245
FbaI TGATCA 1 cut(s) 291
FblI GTMKAC 1 cut(s) 558
Fnu4HI GCNGC 5 cut(s) 393, 752, 1056, 1121, 1241
FokI GGATG 1 cut(s) 136
FriOI GRGCYC 3 cut(s) 131, 598, 1050
Fsp4HI GCNGC 5 cut(s) 393, 752, 1056, 1121, 1241
FspBI CTAG 4 cut(s) 615, 630, 755, 803
GluI GCNGC 5 cut(s) 393, 752, 1056, 1121, 1241
GsuI CTGGAG 3 cut(s) 857, 906, 975
HaeIII GGCC 5 cut(s) 80, 686, 751, 848, 1212
HapII CCGG 4 cut(s) 329, 643, 748, 1209
Hin1II CATG 8 cut(s) 14, 316, 346, 583, 658, 814, 1027, 1227
HinfI GANTC 4 cut(s) 332, 443, 457, 820
HpaII CCGG 4 cut(s) 329, 643, 748, 1209
HphI GGTGA 2 cut(s) 132, 1152
Hpy166II GTNNAC 4 cut(s) 121, 559, 635, 1195
Hpy188I TCNGA 2 cut(s) 337, 517
Hpy188III TCNNGA 7 cut(s) 343, 503, 655, 824, 853, 874, 1085
Hpy8I GTNNAC 4 cut(s) 121, 559, 635, 1195
HpyAV CCTTC 5 cut(s) 57, 83, 447, 859, 1001
HpyCH4III ACNGT 2 cut(s) 859, 1190
HpyCH4V TGCA 7 cut(s) 285, 312, 392, 466, 623, 798, 1243
HpyF10VI GCNNNNNNNGC 2 cut(s) 950, 1209
HpyF3I CTNAG 7 cut(s) 300, 484, 504, 667, 727, 1071, 1124
Hsp92II CATG 8 cut(s) 14, 316, 346, 583, 658, 814, 1027, 1227
Ksp22I TGATCA 1 cut(s) 291
Kzo9I GATC 6 cut(s) 19, 291, 408, 611, 720, 1265
LguI GCTCTTC 1 cut(s) 1038
LmnI GCTCC 3 cut(s) 211, 601, 1154
Lsp1109I GCAGC 4 cut(s) 404, 1067, 1107, 1227
LweI GCATC 3 cut(s) 63, 379, 610
MaeI CTAG 4 cut(s) 615, 630, 755, 803
MaeIII GTNAC 4 cut(s) 375, 493, 788, 898
MalI GATC 6 cut(s) 21, 293, 410, 613, 722, 1267
MboI GATC 6 cut(s) 19, 291, 408, 611, 720, 1265
MboII GAAGA 5 cut(s) 209, 736, 1055, 1264, 1273
MflI RGATCY 2 cut(s) 408, 611
MhlI GDGCHC 3 cut(s) 131, 598, 1050
MluCI AATT 7 cut(s) 90, 105, 287, 307, 571, 706, 1144
MlyI GAGTC 1 cut(s) 437
MseI TTAA 1 cut(s) 89
MslI CAYNNNNRTG 2 cut(s) 475, 1028
MspI CCGG 4 cut(s) 329, 643, 748, 1209
MspR9I CCNGG 3 cut(s) 643, 688, 1209
Mva1269I GAATGC 1 cut(s) 955
MvaI CCWGG 1 cut(s) 688
MwoI GCNNNNNNNGC 2 cut(s) 950, 1209
NciI CCSGG 2 cut(s) 643, 1209
NcoI CCATGG 1 cut(s) 579
NdeI CATATG 1 cut(s) 1245
NdeII GATC 6 cut(s) 19, 291, 408, 611, 720, 1265
NlaIII CATG 8 cut(s) 14, 316, 346, 583, 658, 814, 1027, 1227
NlaIV GGNNCC 3 cut(s) 243, 597, 1156
NmeAIII GCCGAG 1 cut(s) 421
NmuCI GTSAC 3 cut(s) 493, 788, 898
NspI RCATGY 1 cut(s) 1027
PagI TCATGA 2 cut(s) 342, 654
PceI AGGCCT 2 cut(s) 686, 848
PciI ACATGT 1 cut(s) 1023
PciSI GCTCTTC 1 cut(s) 1038
PctI GAATGC 1 cut(s) 955
PfeI GAWTC 3 cut(s) 332, 457, 820
PfoI TCCNGGA 1 cut(s) 641
PkrI GCNGC 5 cut(s) 394, 753, 1057, 1122, 1242
PleI GAGTC 1 cut(s) 437
PpsI GAGTC 1 cut(s) 437
PpuMI RGGWCCY 1 cut(s) 508
PscI ACATGT 1 cut(s) 1023
Psp124BI GAGCTC 2 cut(s) 131, 1050
Psp5II RGGWCCY 1 cut(s) 508
Psp6I CCWGG 1 cut(s) 686
PspGI CCWGG 1 cut(s) 686
PspN4I GGNNCC 3 cut(s) 243, 597, 1156
PspPI GGNCC 5 cut(s) 79, 257, 508, 855, 1211
PspPPI RGGWCCY 1 cut(s) 508
PsuI RGATCY 2 cut(s) 408, 611
RsaI GTAC 3 cut(s) 172, 524, 839
RsaNI GTAC 3 cut(s) 171, 523, 838
RseI CAYNNNNRTG 2 cut(s) 475, 1028
SacI GAGCTC 2 cut(s) 131, 1050
SapI GCTCTTC 1 cut(s) 1038
SaqAI TTAA 1 cut(s) 89
SatI GCNGC 5 cut(s) 393, 752, 1056, 1121, 1241
Sau3AI GATC 6 cut(s) 19, 291, 408, 611, 720, 1265
Sau96I GGNCC 5 cut(s) 79, 257, 508, 855, 1211
ScaI AGTACT 2 cut(s) 172, 524
SchI GAGTC 1 cut(s) 437
ScrFI CCNGG 3 cut(s) 643, 688, 1209
SduI GDGCHC 3 cut(s) 131, 598, 1050
SfaNI GCATC 3 cut(s) 63, 379, 610
SfcI CTRYAG 2 cut(s) 49, 58
SinI GGWCC 3 cut(s) 257, 508, 855
SmiMI CAYNNNNRTG 2 cut(s) 475, 1028
Sse9I AATT 7 cut(s) 90, 105, 287, 307, 571, 706, 1144
SseBI AGGCCT 2 cut(s) 686, 848
SsiI CCGC 2 cut(s) 752, 1163
SspMI CTAG 4 cut(s) 615, 630, 755, 803
SstI GAGCTC 2 cut(s) 131, 1050
StuI AGGCCT 2 cut(s) 686, 848
StyD4I CCNGG 3 cut(s) 641, 686, 1207
StyI CCWWGG 2 cut(s) 426, 579
TaaI ACNGT 2 cut(s) 859, 1190
TaqI TCGA 1 cut(s) 711
TasI AATT 7 cut(s) 90, 105, 287, 307, 571, 706, 1144
TatI WGTACW 2 cut(s) 170, 522
TauI GCSGC 1 cut(s) 754
TfiI GAWTC 3 cut(s) 332, 457, 820
Tru1I TTAA 1 cut(s) 89
Tru9I TTAA 1 cut(s) 89
TscAI CASTG 4 cut(s) 34, 568, 797, 1202
TseFI GTSAC 3 cut(s) 493, 788, 898
TseI GCWGC 4 cut(s) 392, 1055, 1120, 1240
Tsp45I GTSAC 3 cut(s) 493, 788, 898
TspDTI ATGAA 7 cut(s) 17, 329, 331, 359, 671, 780, 1013
TspRI CASTG 4 cut(s) 34, 568, 797, 1202
VpaK11BI GGWCC 3 cut(s) 257, 508, 855
XapI RAATTY 2 cut(s) 706, 1144
XceI RCATGY 1 cut(s) 1027
XcmI CCANNNNNNNNNTGG 2 cut(s) 227, 684
XmiI GTMKAC 1 cut(s) 558
XspI CTAG 4 cut(s) 615, 630, 755, 803
ZrmI AGTACT 2 cut(s) 172, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.