FvH4_6g45680

tryptophan synthase beta chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016013
Physical Location & Seq
Reverse (-)
35007422 .. 35010360
2939 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g45680.t1

Sequence Viewer

Length: 1515 bp
ATGGCTACTCACTGTGTTAATTTTTCTGCAAGTTCATTTCATACTAAGCCTTCTTCATCAGGACCAAGTACCAATGTTGAGAAGAAATGGCTTGGATTTATCACACTTGATAGAAAGCCGAGGAATTTGAAGCTTTCAAAAGGCTGTAGAGCAAGAGCAGCTCTCAATGCTGATACAGTACCAGTCGAAATCCCTCGTCAATGGTACAACCTGATTGCAGATCTGCCTATAAAACCTCCTCCCCCATTGCATCCCAAGACTTTTGAACCTGTTAAACCTGAAGATCTGTCCCCTCTATTTCCTGATGAGTTGATTAAACAGGAGGCAAGTGGTGAGAGATACATAGATATCCCAGAAGAAGTTCGCGAGATATACAAGCTTTGGCGCCCAACCCCTCTCATTAGGGCCAAGAGGTTGGAGAAGCTTCTTGACACGCCGGCCAGAATTTATTACAAATACGAAGGCGGTAGCCCTGCTGGATCACACAAACCCAACAGTGCAGTTCCACAAGTGTATTATAATGCAAAGCAAGGCATCAAGAAGGTTGTGACAGAAACAGGAGCTGGACAATGGGGATGTTCTTTGGCTTTTGCATCCAGCTTATTTGGTCTTGACTGTGAGGTATGGCAAGTGCGAGCTTCATATGATCAGAAACCGTATCGCAAATTGATGATGCAAACTTGGGGGGCAAAGGTACACCCATCTCCATCAAACATAACTGAGTCCGGTAGAAAAACCCTTCTGATGGATCCATCAACCCCGGGAAGCTTAGGAATAGCTATTTCTGAAGCTGTGGAGGTTGCAGCTATGAATGCCGACACTAAATATTGTCTAGGGAGTGTTCTCAATCATGTTTTGCTCCACCAGACTGTAATTGGTGAAGAGTGTCTGAAACAAATGGAAGCAATAGGTGAGACCCCGGACTTGATCATAGGGTGTACTGGTGGAGGATCTAACTTTGCAGGCCTCAGTTTCCCATTCATTAGAGAAAAGCTCAATGGAAAAATGAGCCCTGTAATAAGAGCAGTTGAACCTTCTGCTTGTCCTTCATTAACTCAAGGTGTATACAGATATGATTATGGTGACACAGCAGGGATGACTCCACTAATGAAGATGCATACACTTGGGCATGACTTCATTCCTGACCCAATTCATGCCGGAGGATTGCGCTATCATGGCATGGCTCCACTGATTTCACATGTCTATGAACTCGGTTTCATGGAAGCATTAGCAATTCCCCAGACTGAGTGCTTCAGAGGTGCTATACAATTTGCTAGGTCCGAAGGATTGATTCCAGCACCAGAGCCAACCCATGCCATAGCTGCCACCATTAGGGAGGCTCTGAAGTGTAAAGAATCTGGAGAAGCAAAAGTTATTCTCACTGCAATGTGTGGACATGGCCATTTCGATTTGCCAGCTTACGACAATTACCTACAAGGAAATCTGGTTGACCTGTCATTCGATGATGAGAAGCTACAAGCAGCATTGAGCAGTGTTCCTAACATGCGTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00400 ko01100 ko01110 ko01130 ko01230 map00260 map00400 map01100 map01110 map01130 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

505

Amino Acids

55.32

Weight (kDa)

7.22

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 127 - 465 5.3e-38 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016013)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38530
fragaria_vesca FvH4_6g45680
malus_domestica MD17G1071900.v1.1
prunus_persica Prupe.3G243000_v2.0.a1
pyrus_communis pycom17g07120
rosa_chinensis RchiOBHm_Chr2g0163831
rosa_laevigata RLG00000021419
rosa_multiflora Rmu_sc0005917.1_g000003
rosa_roxburghii Rroxscaffold_2G00086980
rosa_rugosa Rorug02G0504800
rosa_samantha Rh2AG572800 Rh2BG583500 Rh2CG554400 Rh2DG593100
rosa_wichuraiana Rw2G047360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 519
AccB1I GGYRCC 1 cut(s) 384
AccI GTMKAC 1 cut(s) 1065
AccII CGCG 1 cut(s) 366
AciI CCGC 1 cut(s) 465
AclWI GGATC 4 cut(s) 487, 743, 756, 958
AcoI YGGCCR 2 cut(s) 438, 1399
AcsI RAATTY 2 cut(s) 124, 444
AcuI CTGAAG 4 cut(s) 300, 807, 1237, 1364
AcyI GRCGYC 1 cut(s) 385
AfaI GTAC 5 cut(s) 70, 180, 206, 696, 940
AfiI CCNNNNNNNGG 2 cut(s) 745, 1332
AflIII ACRYGT 1 cut(s) 1198
AgsI TTSAA 4 cut(s) 130, 138, 266, 1031
Alw26I GTCTC 1 cut(s) 908
AlwI GGATC 4 cut(s) 487, 743, 756, 958
AlwNI CAGNNNCTG 1 cut(s) 563
Ama87I CYCGRG 1 cut(s) 760
AoxI GGCC 4 cut(s) 405, 438, 964, 1399
ApeKI GCWGC 4 cut(s) 158, 803, 1322, 1481
ApoI RAATTY 2 cut(s) 124, 444
ArsI GACNNNNNNTTYG 2 cut(s) 1442, 1474
Asp700I GAANNNNTTC 1 cut(s) 360
AspLEI GCGC 2 cut(s) 387, 1170
AspS9I GGNCC 3 cut(s) 62, 405, 1278
AsuC2I CCSGG 3 cut(s) 761, 762, 920
AsuHPI GGTGA 4 cut(s) 344, 890, 923, 1094
AvaI CYCGRG 1 cut(s) 760
AvaII GGWCC 2 cut(s) 62, 1278
BalI TGGCCA 1 cut(s) 1401
BamHI GGATCC 1 cut(s) 748
BanI GGYRCC 1 cut(s) 384
BanII GRGCYC 1 cut(s) 1013
BbvI GCAGC 4 cut(s) 170, 815, 1309, 1493
BccI CCATC 4 cut(s) 709, 715, 739, 760
BclI TGATCA 2 cut(s) 646, 927
BcnI CCSGG 3 cut(s) 761, 762, 920
BcoDI GTCTC 1 cut(s) 908
BfaI CTAG 2 cut(s) 833, 1275
BfmI CTRYAG 1 cut(s) 145
BfoI RGCGCY 1 cut(s) 388
BglII AGATCT 2 cut(s) 220, 283
BisI GCNGC 4 cut(s) 159, 804, 1323, 1482
BlsI GCNGC 4 cut(s) 160, 805, 1324, 1483
Bme1390I CCNGG 3 cut(s) 761, 762, 920
Bme18I GGWCC 2 cut(s) 62, 1278
BmeT110I CYCGRG 1 cut(s) 760
BmgT120I GGNCC 3 cut(s) 62, 405, 1278
BmiI GGNNCC 3 cut(s) 386, 750, 1185
BmrFI CCNGG 3 cut(s) 761, 762, 920
BmsI GCATC 5 cut(s) 259, 543, 602, 663, 1104
BplI GAGNNNNNCTC 4 cut(s) 147, 179, 978, 1010
BpmI CTGGAG 1 cut(s) 1380
Bpu10I CCTNAGC 1 cut(s) 769
BpuEI CTTGAG 1 cut(s) 1041
BpuMI CCSGG 3 cut(s) 761, 762, 920
BsaHI GRCGYC 1 cut(s) 385
BsaI GGTCTC 1 cut(s) 908
BsaJI CCNNGG 4 cut(s) 119, 759, 760, 918
BsaWI WCCGGW 1 cut(s) 725
Bsc4I CCNNNNNNNGG 2 cut(s) 745, 1332
Bse118I RCCGGY 1 cut(s) 436
Bse1I ACTGG 2 cut(s) 182, 946
Bse3DI GCAATG 2 cut(s) 245, 1392
BseDI CCNNGG 4 cut(s) 119, 759, 760, 918
BseGI GGATG 4 cut(s) 250, 581, 593, 1101
BseLI CCNNNNNNNGG 2 cut(s) 745, 1332
BseMI GCAATG 2 cut(s) 245, 1392
BseMII CTCAG 3 cut(s) 711, 982, 1236
BseNI ACTGG 2 cut(s) 182, 946
BseRI GAGGAG 1 cut(s) 228
BseXI GCAGC 4 cut(s) 170, 815, 1309, 1493
BsgI GTGCAG 1 cut(s) 519
Bsh1236I CGCG 1 cut(s) 366
BshFI GGCC 4 cut(s) 407, 440, 966, 1401
BshNI GGYRCC 1 cut(s) 384
BsiHKCI CYCGRG 1 cut(s) 760
BsiSI CCGG 5 cut(s) 437, 726, 761, 920, 1158
BslFI GGGAC 1 cut(s) 274
BslI CCNNNNNNNGG 2 cut(s) 745, 1332
BsmAI GTCTC 1 cut(s) 908
BsmFI GGGAC 1 cut(s) 274
BsmI GAATGC 1 cut(s) 817
BsnI GGCC 4 cut(s) 407, 440, 966, 1401
Bso31I GGTCTC 1 cut(s) 908
BsoBI CYCGRG 1 cut(s) 760
Bsp1286I GDGCHC 1 cut(s) 1013
Bsp143I GATC 7 cut(s) 220, 283, 479, 646, 748, 927, 950
Bsp68I TCGCGA 1 cut(s) 366
BspACI CCGC 1 cut(s) 465
BspANI GGCC 4 cut(s) 407, 440, 966, 1401
BspCNI CTCAG 3 cut(s) 712, 981, 1237
BspFNI CGCG 1 cut(s) 366
BspLI GGNNCC 3 cut(s) 386, 750, 1185
BspPI GGATC 4 cut(s) 487, 743, 756, 958
BspT107I GGYRCC 1 cut(s) 384
BspTNI GGTCTC 1 cut(s) 908
BsrDI GCAATG 2 cut(s) 245, 1392
BsrFI RCCGGY 1 cut(s) 436
BsrI ACTGG 2 cut(s) 182, 946
BssAI RCCGGY 1 cut(s) 436
BssECI CCNNGG 4 cut(s) 119, 759, 760, 918
BssMI GATC 7 cut(s) 220, 283, 479, 646, 748, 927, 950
BssNAI GTATAC 1 cut(s) 1066
BssNI GRCGYC 1 cut(s) 385
Bst1107I GTATAC 1 cut(s) 1066
Bst4CI ACNGT 6 cut(s) 14, 178, 497, 617, 657, 871
Bst6I CTCTTC 1 cut(s) 876
BstACI GRCGYC 1 cut(s) 385
BstC8I GCNNGC 4 cut(s) 438, 636, 964, 1416
BstDEI CTNAG 5 cut(s) 45, 720, 769, 968, 1245
BstF5I GGATG 4 cut(s) 250, 581, 593, 1101
BstFNI CGCG 1 cut(s) 366
BstH2I RGCGCY 1 cut(s) 388
BstHHI GCGC 2 cut(s) 387, 1170
BstKTI GATC 7 cut(s) 223, 286, 482, 649, 751, 930, 953
BstMAI GTCTC 1 cut(s) 908
BstMBI GATC 7 cut(s) 220, 283, 479, 646, 748, 927, 950
BstMWI GCNNNNNNNGC 5 cut(s) 158, 167, 812, 1176, 1322
BstNSI RCATGY 2 cut(s) 1202, 1507
BstSCI CCNGG 3 cut(s) 759, 760, 918
BstSFI CTRYAG 1 cut(s) 145
BstUI CGCG 1 cut(s) 366
BstV1I GCAGC 4 cut(s) 170, 815, 1309, 1493
BstX2I RGATCY 4 cut(s) 220, 283, 748, 950
BstXI CCANNNNNNTGG 1 cut(s) 415
BstYI RGATCY 4 cut(s) 220, 283, 748, 950
BstZ17I GTATAC 1 cut(s) 1066
BsuRI GGCC 4 cut(s) 407, 440, 966, 1401
BtsCI GGATG 4 cut(s) 250, 581, 593, 1101
BtsI GCAGTG 2 cut(s) 1380, 1498
BtsIMutI CAGTG 5 cut(s) 10, 502, 1187, 1380, 1498
BtuMI TCGCGA 1 cut(s) 366
Cac8I GCNNGC 4 cut(s) 438, 636, 964, 1416
CaiI CAGNNNCTG 1 cut(s) 563
CfoI GCGC 2 cut(s) 387, 1170
Cfr10I RCCGGY 1 cut(s) 436
Cfr13I GGNCC 3 cut(s) 62, 405, 1278
Cfr9I CCCGGG 1 cut(s) 760
Csp6I GTAC 5 cut(s) 69, 179, 205, 695, 939
CviQI GTAC 5 cut(s) 69, 179, 205, 695, 939
DdeI CTNAG 5 cut(s) 45, 720, 769, 968, 1245
DinI GGCGCC 1 cut(s) 386
DpnI GATC 7 cut(s) 222, 285, 481, 648, 750, 929, 952
DpnII GATC 7 cut(s) 220, 283, 479, 646, 748, 927, 950
EaeI YGGCCR 2 cut(s) 438, 1399
Eam1104I CTCTTC 1 cut(s) 876
EarI CTCTTC 1 cut(s) 876
Eco147I AGGCCT 1 cut(s) 966
Eco24I GRGCYC 1 cut(s) 1013
Eco31I GGTCTC 1 cut(s) 908
Eco32I GATATC 1 cut(s) 349
Eco47I GGWCC 2 cut(s) 62, 1278
Eco57I CTGAAG 4 cut(s) 300, 807, 1237, 1364
Eco88I CYCGRG 1 cut(s) 760
EcoRV GATATC 1 cut(s) 349
EcoT22I ATGCAT 1 cut(s) 1119
EcoT38I GRGCYC 1 cut(s) 1013
EgeI GGCGCC 1 cut(s) 386
EheI GGCGCC 1 cut(s) 386
FaqI GGGAC 1 cut(s) 274
FauNDI CATATG 1 cut(s) 643
FbaI TGATCA 2 cut(s) 646, 927
FblI GTMKAC 1 cut(s) 1065
Fnu4HI GCNGC 4 cut(s) 159, 804, 1323, 1482
FokI GGATG 4 cut(s) 237, 580, 588, 1108
FriOI GRGCYC 1 cut(s) 1013
Fsp4HI GCNGC 4 cut(s) 159, 804, 1323, 1482
FspBI CTAG 2 cut(s) 833, 1275
GlaI GCGC 2 cut(s) 386, 1169
GluI GCNGC 4 cut(s) 159, 804, 1323, 1482
GsuI CTGGAG 1 cut(s) 1380
HaeII RGCGCY 1 cut(s) 388
HaeIII GGCC 4 cut(s) 407, 440, 966, 1401
HapII CCGG 5 cut(s) 437, 726, 761, 920, 1158
HhaI GCGC 2 cut(s) 387, 1170
Hin1I GRCGYC 1 cut(s) 385
Hin6I GCGC 2 cut(s) 385, 1168
HinP1I GCGC 2 cut(s) 385, 1168
HincII GTYRAC 1 cut(s) 1450
HindII GTYRAC 1 cut(s) 1450
HindIII AAGCTT 4 cut(s) 131, 377, 422, 766
HinfI GANTC 4 cut(s) 722, 1099, 1291, 1355
HpaII CCGG 5 cut(s) 437, 726, 761, 920, 1158
HphI GGTGA 4 cut(s) 344, 890, 923, 1094
Hpy166II GTNNAC 5 cut(s) 697, 939, 1066, 1394, 1450
Hpy188I TCNGA 7 cut(s) 651, 744, 787, 891, 1256, 1282, 1344
Hpy188III TCNNGA 8 cut(s) 60, 302, 365, 428, 538, 611, 1142, 1359
Hpy8I GTNNAC 5 cut(s) 697, 939, 1066, 1394, 1450
HpyAV CCTTC 7 cut(s) 60, 455, 535, 749, 1044, 1056, 1277
HpyCH4III ACNGT 6 cut(s) 14, 178, 497, 617, 657, 871
HpyF10VI GCNNNNNNNGC 5 cut(s) 158, 167, 812, 1176, 1322
HpyF3I CTNAG 5 cut(s) 45, 720, 769, 968, 1245
Hsp92I GRCGYC 1 cut(s) 385
HspAI GCGC 2 cut(s) 385, 1168
KasI GGCGCC 1 cut(s) 384
KroI GCCGGC 1 cut(s) 436
KroNI GCCGGC 1 cut(s) 438
Ksp22I TGATCA 2 cut(s) 646, 927
Kzo9I GATC 7 cut(s) 220, 283, 479, 646, 748, 927, 950
LmnI GCTCC 3 cut(s) 560, 864, 1189
Lsp1109I GCAGC 4 cut(s) 170, 815, 1309, 1493
LweI GCATC 5 cut(s) 259, 543, 602, 663, 1104
MaeI CTAG 2 cut(s) 833, 1275
MaeIII GTNAC 2 cut(s) 547, 1082
MalI GATC 7 cut(s) 222, 285, 481, 648, 750, 929, 952
MboI GATC 7 cut(s) 220, 283, 479, 646, 748, 927, 950
MboII GAAGA 6 cut(s) 45, 94, 293, 368, 893, 1123
MflI RGATCY 4 cut(s) 220, 283, 748, 950
MhlI GDGCHC 1 cut(s) 1013
MlsI TGGCCA 1 cut(s) 1401
MluCI AATT 9 cut(s) 19, 124, 444, 665, 873, 1149, 1233, 1268, 1426
MluNI TGGCCA 1 cut(s) 1401
Mly113I GGCGCC 1 cut(s) 385
MlyI GAGTC 2 cut(s) 731, 1093
MmeI TCCRAC 1 cut(s) 396
Mox20I TGGCCA 1 cut(s) 1401
Mph1103I ATGCAT 1 cut(s) 1119
MroNI GCCGGC 1 cut(s) 436
MroXI GAANNNNTTC 1 cut(s) 360
MscI TGGCCA 1 cut(s) 1401
MseI TTAA 4 cut(s) 18, 273, 315, 1052
MslI CAYNNNNRTG 2 cut(s) 1203, 1385
Msp20I TGGCCA 1 cut(s) 1401
MspI CCGG 5 cut(s) 437, 726, 761, 920, 1158
MspR9I CCNGG 3 cut(s) 761, 762, 920
Mva1269I GAATGC 1 cut(s) 817
MvnI CGCG 1 cut(s) 366
MwoI GCNNNNNNNGC 5 cut(s) 158, 167, 812, 1176, 1322
NaeI GCCGGC 1 cut(s) 438
NarI GGCGCC 1 cut(s) 385
NciI CCSGG 3 cut(s) 761, 762, 920
NdeI CATATG 1 cut(s) 643
NdeII GATC 7 cut(s) 220, 283, 479, 646, 748, 927, 950
NgoMIV GCCGGC 1 cut(s) 436
NlaIV GGNNCC 3 cut(s) 386, 750, 1185
NmeAIII GCCGAG 1 cut(s) 144
NmuCI GTSAC 2 cut(s) 547, 1082
NruI TCGCGA 1 cut(s) 366
NsiI ATGCAT 1 cut(s) 1119
NspI RCATGY 2 cut(s) 1202, 1507
PceI AGGCCT 1 cut(s) 966
PciI ACATGT 1 cut(s) 1198
PctI GAATGC 1 cut(s) 817
PdiI GCCGGC 1 cut(s) 438
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 2 cut(s) 1291, 1355
PkrI GCNGC 4 cut(s) 160, 805, 1324, 1483
PleI GAGTC 2 cut(s) 730, 1093
PluTI GGCGCC 1 cut(s) 388
PpsI GAGTC 2 cut(s) 730, 1093
PscI ACATGT 1 cut(s) 1198
PsiI TTATAA 1 cut(s) 519
PspN4I GGNNCC 3 cut(s) 386, 750, 1185
PspPI GGNCC 3 cut(s) 62, 405, 1278
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 4 cut(s) 220, 283, 748, 950
RruI TCGCGA 1 cut(s) 366
RsaI GTAC 5 cut(s) 70, 180, 206, 696, 940
RsaNI GTAC 5 cut(s) 69, 179, 205, 695, 939
RseI CAYNNNNRTG 2 cut(s) 1203, 1385
SaqAI TTAA 4 cut(s) 18, 273, 315, 1052
SatI GCNGC 4 cut(s) 159, 804, 1323, 1482
Sau3AI GATC 7 cut(s) 220, 283, 479, 646, 748, 927, 950
Sau96I GGNCC 3 cut(s) 62, 405, 1278
SchI GAGTC 2 cut(s) 731, 1093
ScrFI CCNGG 3 cut(s) 761, 762, 920
SduI GDGCHC 1 cut(s) 1013
SfaNI GCATC 5 cut(s) 259, 543, 602, 663, 1104
SfcI CTRYAG 1 cut(s) 145
SfoI GGCGCC 1 cut(s) 386
SinI GGWCC 2 cut(s) 62, 1278
SmaI CCCGGG 1 cut(s) 762
SmiMI CAYNNNNRTG 2 cut(s) 1203, 1385
SmlI CTYRAG 1 cut(s) 1056
SmoI CTYRAG 1 cut(s) 1056
Sse9I AATT 9 cut(s) 19, 124, 444, 665, 873, 1149, 1233, 1268, 1426
SseBI AGGCCT 1 cut(s) 966
SsiI CCGC 1 cut(s) 465
SspDI GGCGCC 1 cut(s) 384
SspI AATATT 1 cut(s) 827
SspMI CTAG 2 cut(s) 833, 1275
StuI AGGCCT 1 cut(s) 966
StyD4I CCNGG 3 cut(s) 759, 760, 918
TaaI ACNGT 6 cut(s) 14, 178, 497, 617, 657, 871
TaqI TCGA 3 cut(s) 186, 1407, 1461
TasI AATT 9 cut(s) 19, 124, 444, 665, 873, 1149, 1233, 1268, 1426
TatI WGTACW 1 cut(s) 938
TfiI GAWTC 2 cut(s) 1291, 1355
Tru1I TTAA 4 cut(s) 18, 273, 315, 1052
Tru9I TTAA 4 cut(s) 18, 273, 315, 1052
TscAI CASTG 5 cut(s) 17, 502, 1194, 1387, 1498
TseFI GTSAC 2 cut(s) 547, 1082
TseI GCWGC 4 cut(s) 158, 803, 1322, 1481
Tsp45I GTSAC 2 cut(s) 547, 1082
TspMI CCCGGG 1 cut(s) 760
TspRI CASTG 5 cut(s) 17, 502, 1194, 1387, 1498
VpaK11BI GGWCC 2 cut(s) 62, 1278
XapI RAATTY 2 cut(s) 124, 444
XceI RCATGY 2 cut(s) 1202, 1507
XcmI CCANNNNNNNNNTGG 1 cut(s) 872
XmaI CCCGGG 1 cut(s) 760
XmiI GTMKAC 1 cut(s) 1065
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 2 cut(s) 833, 1275
Zsp2I ATGCAT 1 cut(s) 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.