Rroxscaffold_2G00086980

tryptophan synthase beta chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016013
Physical Location & Seq
Forward (+)
9091372 .. 9094024
2653 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00086980.1

Sequence Viewer

Length: 1440 bp
ATGGCTACTCACTGTGTTAATTTCTGTGCAACTTCATTTGATGCTAAGCCTTCTTCATCAGAATCACACTCCAGAGTTGAGAAGAAATGGCTTGGATCTATTGCACTTGATAGAAAGCCGAAAAATTTGAAGCTTTCAAATGGCTGTAGAGCAAGAGCAGCCCTCAATGCTGATTCAATACCAATCGAAATCCCTCGTCAGTGGTACAACCTGATTGCAGATCTTCCTATAAAACCTCCTCCTTCATTGCATCCCAAGACTTTTGAACCAGTAAAACCTGAAGATCTGTCCCCTCTATTTCCTGATGAGTTGATTAAACAGGAAGCAAGTGGTGAAAGATACATAGATATCCCAGAAGAAGTTCGAGAGATATACAAGCTTTGGCGTCCAACCCCTCTCATTAGGGCAAAGAGGTTGGAGAAGCTTCTTGACACGCCTGCCAGAATTTATTACAAGTATGAAGGTGTTAGCCCTGCTGGATCACACAAACCCAACACTGCAGTTCCTCAAGTGTATTATAATGCACAACAAGGCATCAAGAAGGTTGTGACAGAAACAGGAGCTGGTCAATGGGGATGTTCTTTGGCTTTTGCATCCAGCTTATTCGGTCTTGAATGTGAGGTATGGCAAGTGCGTGCTTCGTATGATCAGAAACCATATCGCAAATTGATGATGCAAACTTGGGGGCAAAGTTTAGGAATAGCTATTTCTGAAGCTGTGGAGGTTGCAGCTATGAATGCTGACACTAAATACTGTCTGGGAAGTGTTCTCAATCATGTTTTGCTCCACCAGACTGTAATAGGTGAAGAGTGTCTGAAACAAATGGAAGCGATAGGTGAAACCCCGGACTTGATCATAGGGTGTACTGGTGGAGGATCCAACTTTGCAGGGCTAAGTTTCCCATTCATTAGAGAAAAGCTTGATGGGAAAATGAGCCCTGTAATTAGAGCAGTTGAGCCTTCTGCTTGTCCTTCATTAACTAAAGGTGTATACAGATACGATTATGGTGACACAGCAGGGATGACTCCACTAATGAAGATGCATACACTTGGGCATGACTTCATTCCTGACCCAATTCATGCCGGAGGATTGCGTTATCATGGAATGGCTCCATTGATTTCACACGTGTATGAACTCGGTTTCATGGAAGCAATTTCAATTCCCCAGACTGAGTGCTTCAAAGGTGCTATACAATTTGCTAGGTCCGAAGGATTGATTCCGGCGCCAGAGCCAACCCATGCCATAGCTGCCACCATTAGGGAGGCTCTGAAGTGTAAAGAGACCGGAGAAGCAAAAGTTATTCTCACTGCAATGTGTGGACATGGCCATTTCGATTTGCCAGCTTATGACAATTATCTGCAAGGAAATCTGGTTGACCTGTCATTCGAGGATGAGAAGCTACAAGCAGCACTGGGTAGTGTTCCTAACGTAGGGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00400 ko01100 ko01110 ko01130 ko01230 map00260 map00400 map01100 map01110 map01130 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

479

Amino Acids

52.66

Weight (kDa)

6.29

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 127 - 440 5.4e-37 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016013)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38530
fragaria_vesca FvH4_6g45680
malus_domestica MD17G1071900.v1.1
prunus_persica Prupe.3G243000_v2.0.a1
pyrus_communis pycom17g07120
rosa_chinensis RchiOBHm_Chr2g0163831
rosa_laevigata RLG00000021419
rosa_multiflora Rmu_sc0005917.1_g000003
rosa_roxburghii Rroxscaffold_2G00086980
rosa_rugosa Rorug02G0504800
rosa_samantha Rh2AG572800 Rh2BG583500 Rh2CG554400 Rh2DG593100
rosa_wichuraiana Rw2G047360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 519
AccB1I GGYRCC 1 cut(s) 1222
AccI GTMKAC 1 cut(s) 990
AclWI GGATC 4 cut(s) 103, 487, 870, 883
AcoI YGGCCR 1 cut(s) 1324
AcsI RAATTY 2 cut(s) 124, 444
AcuI CTGAAG 3 cut(s) 300, 732, 1289
AcvI CACGTG 1 cut(s) 1126
AcyI GRCGYC 2 cut(s) 385, 1223
AfaI GTAC 2 cut(s) 206, 865
AfiI CCNNNNNNNGG 2 cut(s) 1257, 1430
AflIII ACRYGT 2 cut(s) 1123, 1125
AgsI TTSAA 7 cut(s) 130, 138, 177, 266, 614, 1158, 1180
Alw26I GTCTC 1 cut(s) 1274
AlwI GGATC 4 cut(s) 103, 487, 870, 883
AlwNI CAGNNNCTG 1 cut(s) 563
AoxI GGCC 1 cut(s) 1324
ApeKI GCWGC 4 cut(s) 158, 728, 1247, 1406
ApoI RAATTY 2 cut(s) 124, 444
ArsI GACNNNNNNTTYG 2 cut(s) 1367, 1399
Asp700I GAANNNNTTC 1 cut(s) 360
AspLEI GCGC 1 cut(s) 1225
AspS9I GGNCC 1 cut(s) 1203
AsuC2I CCSGG 1 cut(s) 845
AsuHPI GGTGA 4 cut(s) 344, 815, 848, 1019
AvaII GGWCC 1 cut(s) 1203
BalI TGGCCA 1 cut(s) 1326
BamHI GGATCC 1 cut(s) 875
BanI GGYRCC 1 cut(s) 1222
BanII GRGCYC 1 cut(s) 938
BbrPI CACGTG 1 cut(s) 1126
BbvI GCAGC 4 cut(s) 170, 740, 1234, 1418
BccI CCATC 1 cut(s) 917
BclI TGATCA 2 cut(s) 646, 852
BcnI CCSGG 1 cut(s) 845
BcoDI GTCTC 1 cut(s) 1274
BfaI CTAG 1 cut(s) 1200
BfmI CTRYAG 2 cut(s) 145, 498
BfoI RGCGCY 1 cut(s) 1226
BglII AGATCT 2 cut(s) 220, 283
BisI GCNGC 4 cut(s) 159, 729, 1248, 1407
BlpI GCTNAGC 1 cut(s) 45
BlsI GCNGC 4 cut(s) 160, 730, 1249, 1408
Bme1390I CCNGG 1 cut(s) 845
Bme18I GGWCC 1 cut(s) 1203
BmgT120I GGNCC 1 cut(s) 1203
BmiI GGNNCC 3 cut(s) 877, 1110, 1224
BmrFI CCNGG 1 cut(s) 845
BmrI ACTGGG 1 cut(s) 1421
BmsI GCATC 6 cut(s) 31, 259, 543, 602, 663, 1029
BmuI ACTGGG 1 cut(s) 1421
BplI GAGNNNNNCTC 2 cut(s) 147, 179
BpmI CTGGAG 1 cut(s) 55
Bpu1102I GCTNAGC 1 cut(s) 45
BpuEI CTTGAG 1 cut(s) 492
BpuMI CCSGG 1 cut(s) 845
BsaAI YACGTR 1 cut(s) 1126
BsaHI GRCGYC 2 cut(s) 385, 1223
BsaI GGTCTC 1 cut(s) 1274
BsaJI CCNNGG 1 cut(s) 843
BsaWI WCCGGW 1 cut(s) 1283
Bsc4I CCNNNNNNNGG 2 cut(s) 1257, 1430
Bse1I ACTGG 3 cut(s) 269, 871, 1416
Bse3DI GCAATG 2 cut(s) 245, 1317
BseDI CCNNGG 1 cut(s) 843
BseGI GGATG 5 cut(s) 250, 581, 593, 1026, 1396
BseLI CCNNNNNNNGG 2 cut(s) 1257, 1430
BseMI GCAATG 2 cut(s) 245, 1317
BseMII CTCAG 1 cut(s) 1161
BseNI ACTGG 3 cut(s) 269, 871, 1416
BseRI GAGGAG 1 cut(s) 228
BseXI GCAGC 4 cut(s) 170, 740, 1234, 1418
BshFI GGCC 1 cut(s) 1326
BshNI GGYRCC 1 cut(s) 1222
BsiSI CCGG 4 cut(s) 845, 1083, 1220, 1284
BslFI GGGAC 1 cut(s) 274
BslI CCNNNNNNNGG 2 cut(s) 1257, 1430
BsmAI GTCTC 1 cut(s) 1274
BsmFI GGGAC 1 cut(s) 274
BsmI GAATGC 1 cut(s) 742
BsnI GGCC 1 cut(s) 1326
Bso31I GGTCTC 1 cut(s) 1274
Bsp1286I GDGCHC 1 cut(s) 938
Bsp143I GATC 7 cut(s) 95, 220, 283, 479, 646, 852, 875
Bsp1720I GCTNAGC 1 cut(s) 45
BspANI GGCC 1 cut(s) 1326
BspCNI CTCAG 1 cut(s) 1162
BspLI GGNNCC 3 cut(s) 877, 1110, 1224
BspMAI CTGCAG 1 cut(s) 502
BspPI GGATC 4 cut(s) 103, 487, 870, 883
BspT107I GGYRCC 1 cut(s) 1222
BspTNI GGTCTC 1 cut(s) 1274
BsrDI GCAATG 2 cut(s) 245, 1317
BsrI ACTGG 3 cut(s) 269, 871, 1416
BssECI CCNNGG 1 cut(s) 843
BssMI GATC 7 cut(s) 95, 220, 283, 479, 646, 852, 875
BssNAI GTATAC 1 cut(s) 991
BssNI GRCGYC 2 cut(s) 385, 1223
Bst1107I GTATAC 1 cut(s) 991
Bst4CI ACNGT 3 cut(s) 14, 755, 796
Bst6I CTCTTC 1 cut(s) 801
BstACI GRCGYC 2 cut(s) 385, 1223
BstBAI YACGTR 1 cut(s) 1126
BstC8I GCNNGC 3 cut(s) 438, 636, 1341
BstDEI CTNAG 3 cut(s) 45, 893, 1170
BstENI CCTNNNNNAGG 1 cut(s) 1428
BstF5I GGATG 5 cut(s) 250, 581, 593, 1026, 1396
BstH2I RGCGCY 1 cut(s) 1226
BstHHI GCGC 1 cut(s) 1225
BstKTI GATC 7 cut(s) 98, 223, 286, 482, 649, 855, 878
BstMAI GTCTC 1 cut(s) 1274
BstMBI GATC 7 cut(s) 95, 220, 283, 479, 646, 852, 875
BstMWI GCNNNNNNNGC 4 cut(s) 158, 167, 737, 1247
BstSCI CCNGG 1 cut(s) 843
BstSFI CTRYAG 2 cut(s) 145, 498
BstV1I GCAGC 4 cut(s) 170, 740, 1234, 1418
BstX2I RGATCY 4 cut(s) 95, 220, 283, 875
BstYI RGATCY 4 cut(s) 95, 220, 283, 875
BstZ17I GTATAC 1 cut(s) 991
BsuRI GGCC 1 cut(s) 1326
BtsCI GGATG 5 cut(s) 250, 581, 593, 1026, 1396
BtsI GCAGTG 2 cut(s) 495, 1305
BtsIMutI CAGTG 5 cut(s) 10, 206, 495, 1305, 1409
Cac8I GCNNGC 3 cut(s) 438, 636, 1341
CaiI CAGNNNCTG 1 cut(s) 563
CfoI GCGC 1 cut(s) 1225
Cfr13I GGNCC 1 cut(s) 1203
CseI GACGC 1 cut(s) 374
Csp6I GTAC 2 cut(s) 205, 864
CviAII CATG 7 cut(s) 776, 1055, 1079, 1100, 1144, 1238, 1322
CviQI GTAC 2 cut(s) 205, 864
DdeI CTNAG 3 cut(s) 45, 893, 1170
DinI GGCGCC 1 cut(s) 1224
DpnI GATC 7 cut(s) 97, 222, 285, 481, 648, 854, 877
DpnII GATC 7 cut(s) 95, 220, 283, 479, 646, 852, 875
EaeI YGGCCR 1 cut(s) 1324
Eam1104I CTCTTC 1 cut(s) 801
EarI CTCTTC 1 cut(s) 801
Eco24I GRGCYC 1 cut(s) 938
Eco31I GGTCTC 1 cut(s) 1274
Eco32I GATATC 1 cut(s) 349
Eco47I GGWCC 1 cut(s) 1203
Eco57I CTGAAG 3 cut(s) 300, 732, 1289
Eco72I CACGTG 1 cut(s) 1126
EcoNI CCTNNNNNAGG 1 cut(s) 1428
EcoRV GATATC 1 cut(s) 349
EcoT22I ATGCAT 1 cut(s) 1044
EcoT38I GRGCYC 1 cut(s) 938
EgeI GGCGCC 1 cut(s) 1224
EheI GGCGCC 1 cut(s) 1224
FaeI CATG 7 cut(s) 779, 1058, 1082, 1103, 1147, 1241, 1325
FaqI GGGAC 1 cut(s) 274
FatI CATG 7 cut(s) 775, 1054, 1078, 1099, 1143, 1237, 1321
FbaI TGATCA 2 cut(s) 646, 852
FblI GTMKAC 1 cut(s) 990
Fnu4HI GCNGC 4 cut(s) 159, 729, 1248, 1407
FokI GGATG 5 cut(s) 237, 580, 588, 1033, 1403
FriOI GRGCYC 1 cut(s) 938
Fsp4HI GCNGC 4 cut(s) 159, 729, 1248, 1407
FspBI CTAG 1 cut(s) 1200
GlaI GCGC 1 cut(s) 1224
GluI GCNGC 4 cut(s) 159, 729, 1248, 1407
GsuI CTGGAG 1 cut(s) 55
HaeII RGCGCY 1 cut(s) 1226
HaeIII GGCC 1 cut(s) 1326
HapII CCGG 4 cut(s) 845, 1083, 1220, 1284
HgaI GACGC 1 cut(s) 374
HhaI GCGC 1 cut(s) 1225
Hin1I GRCGYC 2 cut(s) 385, 1223
Hin1II CATG 7 cut(s) 779, 1058, 1082, 1103, 1147, 1241, 1325
Hin6I GCGC 1 cut(s) 1223
HinP1I GCGC 1 cut(s) 1223
HincII GTYRAC 1 cut(s) 1375
HindII GTYRAC 1 cut(s) 1375
HindIII AAGCTT 4 cut(s) 131, 377, 422, 917
HinfI GANTC 4 cut(s) 62, 173, 1024, 1216
HpaII CCGG 4 cut(s) 845, 1083, 1220, 1284
HphI GGTGA 4 cut(s) 344, 815, 848, 1019
Hpy166II GTNNAC 4 cut(s) 864, 991, 1319, 1375
Hpy188I TCNGA 6 cut(s) 61, 651, 712, 816, 1207, 1269
Hpy188III TCNNGA 7 cut(s) 72, 302, 365, 428, 538, 611, 1067
Hpy8I GTNNAC 4 cut(s) 864, 991, 1319, 1375
HpyAV CCTTC 7 cut(s) 60, 252, 455, 535, 969, 981, 1202
HpyCH4III ACNGT 3 cut(s) 14, 755, 796
HpyCH4IV ACGT 2 cut(s) 1125, 1428
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 167, 737, 1247
HpyF3I CTNAG 3 cut(s) 45, 893, 1170
HpySE526I ACGT 2 cut(s) 1125, 1428
Hsp92I GRCGYC 2 cut(s) 385, 1223
Hsp92II CATG 7 cut(s) 779, 1058, 1082, 1103, 1147, 1241, 1325
HspAI GCGC 1 cut(s) 1223
KasI GGCGCC 1 cut(s) 1222
Ksp22I TGATCA 2 cut(s) 646, 852
Kzo9I GATC 7 cut(s) 95, 220, 283, 479, 646, 852, 875
LmnI GCTCC 4 cut(s) 560, 789, 1114, 1434
Lsp1109I GCAGC 4 cut(s) 170, 740, 1234, 1418
LweI GCATC 6 cut(s) 31, 259, 543, 602, 663, 1029
MaeI CTAG 1 cut(s) 1200
MaeII ACGT 2 cut(s) 1125, 1428
MaeIII GTNAC 2 cut(s) 547, 1007
MalI GATC 7 cut(s) 97, 222, 285, 481, 648, 854, 877
MboI GATC 7 cut(s) 95, 220, 283, 479, 646, 852, 875
MboII GAAGA 7 cut(s) 45, 94, 215, 293, 368, 818, 1048
MflI RGATCY 4 cut(s) 95, 220, 283, 875
MhlI GDGCHC 1 cut(s) 938
MlsI TGGCCA 1 cut(s) 1326
MluNI TGGCCA 1 cut(s) 1326
Mly113I GGCGCC 1 cut(s) 1223
MlyI GAGTC 1 cut(s) 1018
MmeI TCCRAC 3 cut(s) 396, 413, 903
Mox20I TGGCCA 1 cut(s) 1326
Mph1103I ATGCAT 1 cut(s) 1044
MroXI GAANNNNTTC 1 cut(s) 360
MscI TGGCCA 1 cut(s) 1326
MseI TTAA 3 cut(s) 18, 315, 977
MslI CAYNNNNRTG 2 cut(s) 1128, 1310
Msp20I TGGCCA 1 cut(s) 1326
MspI CCGG 4 cut(s) 845, 1083, 1220, 1284
MspR9I CCNGG 1 cut(s) 845
Mva1269I GAATGC 1 cut(s) 742
MwoI GCNNNNNNNGC 4 cut(s) 158, 167, 737, 1247
NarI GGCGCC 1 cut(s) 1223
NciI CCSGG 1 cut(s) 845
NdeII GATC 7 cut(s) 95, 220, 283, 479, 646, 852, 875
NlaIII CATG 7 cut(s) 779, 1058, 1082, 1103, 1147, 1241, 1325
NlaIV GGNNCC 3 cut(s) 877, 1110, 1224
NmuCI GTSAC 2 cut(s) 547, 1007
NsiI ATGCAT 1 cut(s) 1044
PctI GAATGC 1 cut(s) 742
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 3 cut(s) 62, 173, 1216
PkrI GCNGC 4 cut(s) 160, 730, 1249, 1408
PleI GAGTC 1 cut(s) 1018
PluTI GGCGCC 1 cut(s) 1226
PmaCI CACGTG 1 cut(s) 1126
PmlI CACGTG 1 cut(s) 1126
PpsI GAGTC 1 cut(s) 1018
Ppu21I YACGTR 1 cut(s) 1126
PsiI TTATAA 1 cut(s) 519
PspCI CACGTG 1 cut(s) 1126
PspN4I GGNNCC 3 cut(s) 877, 1110, 1224
PspPI GGNCC 1 cut(s) 1203
PstI CTGCAG 1 cut(s) 502
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 4 cut(s) 95, 220, 283, 875
RsaI GTAC 2 cut(s) 206, 865
RsaNI GTAC 2 cut(s) 205, 864
RseI CAYNNNNRTG 2 cut(s) 1128, 1310
SaqAI TTAA 3 cut(s) 18, 315, 977
SatI GCNGC 4 cut(s) 159, 729, 1248, 1407
Sau3AI GATC 7 cut(s) 95, 220, 283, 479, 646, 852, 875
Sau96I GGNCC 1 cut(s) 1203
SchI GAGTC 1 cut(s) 1018
ScrFI CCNGG 1 cut(s) 845
SduI GDGCHC 1 cut(s) 938
SfaNI GCATC 6 cut(s) 31, 259, 543, 602, 663, 1029
SfcI CTRYAG 2 cut(s) 145, 498
SfoI GGCGCC 1 cut(s) 1224
SinI GGWCC 1 cut(s) 1203
SmiMI CAYNNNNRTG 2 cut(s) 1128, 1310
SmlI CTYRAG 1 cut(s) 507
SmoI CTYRAG 1 cut(s) 507
SspDI GGCGCC 1 cut(s) 1222
SspMI CTAG 1 cut(s) 1200
StyD4I CCNGG 1 cut(s) 843
TaaI ACNGT 3 cut(s) 14, 755, 796
TaiI ACGT 2 cut(s) 1128, 1431
TaqI TCGA 4 cut(s) 186, 364, 1332, 1386
TaqII GACCGA 1 cut(s) 596
TatI WGTACW 1 cut(s) 863
TfiI GAWTC 3 cut(s) 62, 173, 1216
Tru1I TTAA 3 cut(s) 18, 315, 977
Tru9I TTAA 3 cut(s) 18, 315, 977
TscAI CASTG 5 cut(s) 17, 206, 502, 1312, 1416
TseFI GTSAC 2 cut(s) 547, 1007
TseI GCWGC 4 cut(s) 158, 728, 1247, 1406
Tsp45I GTSAC 2 cut(s) 547, 1007
TspRI CASTG 5 cut(s) 17, 206, 502, 1312, 1416
VpaK11BI GGWCC 1 cut(s) 1203
XagI CCTNNNNNAGG 1 cut(s) 1428
XapI RAATTY 2 cut(s) 124, 444
XmiI GTMKAC 1 cut(s) 990
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 1 cut(s) 1200
Zsp2I ATGCAT 1 cut(s) 1044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.