Rh2AG572800

tryptophan synthase beta chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0016013
Physical Location & Seq
Reverse (-)
80316015 .. 80319170
3156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG572800.1

Sequence Viewer

Length: 1515 bp
ATGGCTACTCACTGTGTTAATTTCTGTGCAAGTTCATTTGATGCTAAGCCTTCTTCATCAGGATCACACTCCAGAGTTGAGAAGAAATGGCTTGGATCTATTGCACTTGATAGAAAGCCGAAGAATTTGAAGCTTTCAAATGGCTGTAGAGCAAGAGCAGCCCTCAATGCTGATTCAATACCAATCGAAATCCCTCGTCAGTGGTACAACCTGATTGCAGATCTTCCTATAAAACCTCCTCCTTCATTGCATCCCAAAACTTTTGAACCAGTAAAACCTGAAGATCTGTCCCCTCTATTTCCTGATGAGTTGATTAAACAGGAAGCAAGTGGTGAAAGATACATAGATATCCCAGAAGAAGTTCGAGAGATATACAAGCTTTGGCGTCCAACCCCTCTCATTAGGGCAAAGAGGTTGGAGAAGCTTCTTGACACGCCTGCCAGAATTTATTACAAGTATGAAGGTGTTAGCCCTGCTGGATCACACAAACCCAACACTGCAGTTCCTCAAGTGTATTATAATGCACAACAAGGCATCAAGAAGGTTGTGACAGAAACAGGAGCTGGTCAATGGGGATGTTCGTTGGCTTTTGCATCCAGCTTATTCGGTCTTGACTGTGAGGTATGGCAAGTGCGTGCTTCGTATGATCAGAAACCATATCGCAAATTGATGATGCAAACTTGGGGGGCAAAGGTACACCCATCTCCATCGAACATAACTGAATCAGGTAGAAAAATCCTTCTGATGGATCCATCGACCCCGGGAAGTTTAGGAATAGCTATTTCTGAAGCTGTGGAGGTTGCAGCTATGAATGCTGACACTAAATACTGTCTGGGAAGTGTTCTCAATCATGTTTTGCTCCACCAGACTGTAATAGGTGAAGAGTGTCTGAAACAAATGGAAGCGATAGGTGAAACCCCGGACTTGATCATAGGGTGTACTGGTGGAGGATCCAACTTTGCAGGGCTAAGTTTCCCATTCATTAGAGAAAAGCTCGATGGGAAAATGAGCCCTGTAATTAGAGCAGTTGAGCCTTCTGCTTGTCCTTCATTAACTAAAGGTGTATACAGATACGATTATGGGGACACAGCAGGGATGACTCCACTAATGAAGATGCATACACTTGGGCATGACTTCATTCCTGATCCAATTCATGCCGGAGGATTGCGTTATCATGGAATGGCTCCATTGATTTCACACGTCTATGAACTCGGTTTCATGGAAGCAATTTCAATTCCCCAGATTGAGTGCTTCAAAGGTGCTATACAATTTGCTAGGTCTGAAGGATTGATTCCAGCGCCAGAGCCAACCCATGCCATAGCTGCCACCATTAGGGAGGCTCTGAAGTGTAAAGAGACCGGAGAAGCAAAAGTTATTCTCACTGCAATGTGTGGACATGGCCATTTCGATTTGCCAGCTTATGACAATTATCTGCAAGGAAATCTGGTTGACCTGTCATTCGAGGATGAGAAGCTACAAGCAGCACTGGGTAGTGTTCCTAACGTAGGGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00400 ko01100 ko01110 ko01130 ko01230 map00260 map00400 map01100 map01110 map01130 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

504

Amino Acids

55.16

Weight (kDa)

6.58

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 127 - 465 3.3e-38 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016013)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38530
fragaria_vesca FvH4_6g45680
malus_domestica MD17G1071900.v1.1
prunus_persica Prupe.3G243000_v2.0.a1
pyrus_communis pycom17g07120
rosa_chinensis RchiOBHm_Chr2g0163831
rosa_laevigata RLG00000021419
rosa_multiflora Rmu_sc0005917.1_g000003
rosa_roxburghii Rroxscaffold_2G00086980
rosa_rugosa Rorug02G0504800
rosa_samantha Rh2AG572800 Rh2BG583500 Rh2CG554400 Rh2DG593100
rosa_wichuraiana Rw2G047360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 519
AccI GTMKAC 1 cut(s) 1065
AclWI GGATC 8 cut(s) 70, 103, 487, 743, 756, 945, 958, 1139
AcoI YGGCCR 1 cut(s) 1399
AcsI RAATTY 2 cut(s) 124, 444
AcuI CTGAAG 4 cut(s) 300, 807, 1302, 1364
AcyI GRCGYC 1 cut(s) 385
AfaI GTAC 3 cut(s) 206, 696, 940
AfiI CCNNNNNNNGG 3 cut(s) 745, 1332, 1505
AflIII ACRYGT 1 cut(s) 1198
AgsI TTSAA 6 cut(s) 130, 138, 177, 266, 1233, 1255
AjiI CACGTC 1 cut(s) 1201
Alw26I GTCTC 1 cut(s) 1349
AlwI GGATC 8 cut(s) 70, 103, 487, 743, 756, 945, 958, 1139
AlwNI CAGNNNCTG 1 cut(s) 563
Ama87I CYCGRG 1 cut(s) 760
AoxI GGCC 1 cut(s) 1399
ApeKI GCWGC 4 cut(s) 158, 803, 1322, 1481
ApoI RAATTY 2 cut(s) 124, 444
ArsI GACNNNNNNTTYG 2 cut(s) 1442, 1474
Asp700I GAANNNNTTC 1 cut(s) 360
AspLEI GCGC 1 cut(s) 1300
AsuC2I CCSGG 3 cut(s) 761, 762, 920
AsuHPI GGTGA 3 cut(s) 344, 890, 923
AvaI CYCGRG 1 cut(s) 760
BalI TGGCCA 1 cut(s) 1401
BamHI GGATCC 2 cut(s) 748, 950
BanII GRGCYC 1 cut(s) 1013
BbvI GCAGC 4 cut(s) 170, 815, 1309, 1493
BccI CCATC 5 cut(s) 709, 715, 739, 760, 992
BclI TGATCA 2 cut(s) 646, 927
BcnI CCSGG 3 cut(s) 761, 762, 920
BcoDI GTCTC 1 cut(s) 1349
BfaI CTAG 1 cut(s) 1275
BfmI CTRYAG 2 cut(s) 145, 498
BfoI RGCGCY 1 cut(s) 1301
BglII AGATCT 2 cut(s) 220, 283
BisI GCNGC 4 cut(s) 159, 804, 1323, 1482
BlpI GCTNAGC 1 cut(s) 45
BlsI GCNGC 4 cut(s) 160, 805, 1324, 1483
Bme1390I CCNGG 3 cut(s) 761, 762, 920
BmeT110I CYCGRG 1 cut(s) 760
BmgBI CACGTC 1 cut(s) 1201
BmiI GGNNCC 3 cut(s) 750, 952, 1185
BmrFI CCNGG 3 cut(s) 761, 762, 920
BmrI ACTGGG 1 cut(s) 1496
BmsI GCATC 6 cut(s) 31, 259, 543, 602, 663, 1104
BmuI ACTGGG 1 cut(s) 1496
BplI GAGNNNNNCTC 4 cut(s) 147, 179, 978, 1010
BpmI CTGGAG 1 cut(s) 55
Bpu1102I GCTNAGC 1 cut(s) 45
BpuEI CTTGAG 1 cut(s) 492
BpuMI CCSGG 3 cut(s) 761, 762, 920
BsaHI GRCGYC 1 cut(s) 385
BsaI GGTCTC 1 cut(s) 1349
BsaJI CCNNGG 3 cut(s) 759, 760, 918
BsaWI WCCGGW 1 cut(s) 1358
Bsc4I CCNNNNNNNGG 3 cut(s) 745, 1332, 1505
Bse1I ACTGG 3 cut(s) 269, 946, 1491
Bse3DI GCAATG 2 cut(s) 245, 1392
BseDI CCNNGG 3 cut(s) 759, 760, 918
BseGI GGATG 5 cut(s) 250, 581, 593, 1101, 1471
BseLI CCNNNNNNNGG 3 cut(s) 745, 1332, 1505
BseMI GCAATG 2 cut(s) 245, 1392
BseNI ACTGG 3 cut(s) 269, 946, 1491
BseRI GAGGAG 1 cut(s) 228
BseXI GCAGC 4 cut(s) 170, 815, 1309, 1493
BshFI GGCC 1 cut(s) 1401
BsiHKCI CYCGRG 1 cut(s) 760
BsiSI CCGG 4 cut(s) 761, 920, 1158, 1359
BslFI GGGAC 2 cut(s) 274, 1097
BslI CCNNNNNNNGG 3 cut(s) 745, 1332, 1505
BsmAI GTCTC 1 cut(s) 1349
BsmFI GGGAC 2 cut(s) 274, 1097
BsmI GAATGC 1 cut(s) 817
BsnI GGCC 1 cut(s) 1401
Bso31I GGTCTC 1 cut(s) 1349
BsoBI CYCGRG 1 cut(s) 760
Bsp1286I GDGCHC 1 cut(s) 1013
Bsp1720I GCTNAGC 1 cut(s) 45
BspANI GGCC 1 cut(s) 1401
BspLI GGNNCC 3 cut(s) 750, 952, 1185
BspMAI CTGCAG 1 cut(s) 502
BspPI GGATC 8 cut(s) 70, 103, 487, 743, 756, 945, 958, 1139
BspTNI GGTCTC 1 cut(s) 1349
BsrDI GCAATG 2 cut(s) 245, 1392
BsrI ACTGG 3 cut(s) 269, 946, 1491
BssECI CCNNGG 3 cut(s) 759, 760, 918
BssNAI GTATAC 1 cut(s) 1066
BssNI GRCGYC 1 cut(s) 385
Bst1107I GTATAC 1 cut(s) 1066
Bst4CI ACNGT 4 cut(s) 14, 617, 830, 871
Bst6I CTCTTC 1 cut(s) 876
BstACI GRCGYC 1 cut(s) 385
BstC8I GCNNGC 3 cut(s) 438, 636, 1416
BstDEI CTNAG 2 cut(s) 45, 968
BstENI CCTNNNNNAGG 1 cut(s) 1503
BstF5I GGATG 5 cut(s) 250, 581, 593, 1101, 1471
BstH2I RGCGCY 1 cut(s) 1301
BstHHI GCGC 1 cut(s) 1300
BstMAI GTCTC 1 cut(s) 1349
BstMWI GCNNNNNNNGC 4 cut(s) 158, 167, 812, 1322
BstSCI CCNGG 3 cut(s) 759, 760, 918
BstSFI CTRYAG 2 cut(s) 145, 498
BstV1I GCAGC 4 cut(s) 170, 815, 1309, 1493
BstX2I RGATCY 5 cut(s) 95, 220, 283, 748, 950
BstYI RGATCY 5 cut(s) 95, 220, 283, 748, 950
BstZ17I GTATAC 1 cut(s) 1066
BsuRI GGCC 1 cut(s) 1401
BtrI CACGTC 1 cut(s) 1201
BtsCI GGATG 5 cut(s) 250, 581, 593, 1101, 1471
BtsI GCAGTG 2 cut(s) 495, 1380
BtsIMutI CAGTG 5 cut(s) 10, 206, 495, 1380, 1484
Cac8I GCNNGC 3 cut(s) 438, 636, 1416
CaiI CAGNNNCTG 1 cut(s) 563
CfoI GCGC 1 cut(s) 1300
Cfr9I CCCGGG 1 cut(s) 760
CseI GACGC 1 cut(s) 374
Csp6I GTAC 3 cut(s) 205, 695, 939
CviAII CATG 7 cut(s) 851, 1130, 1154, 1175, 1219, 1313, 1397
CviQI GTAC 3 cut(s) 205, 695, 939
DdeI CTNAG 2 cut(s) 45, 968
EaeI YGGCCR 1 cut(s) 1399
Eam1104I CTCTTC 1 cut(s) 876
EarI CTCTTC 1 cut(s) 876
Eco24I GRGCYC 1 cut(s) 1013
Eco31I GGTCTC 1 cut(s) 1349
Eco32I GATATC 1 cut(s) 349
Eco57I CTGAAG 4 cut(s) 300, 807, 1302, 1364
Eco88I CYCGRG 1 cut(s) 760
EcoNI CCTNNNNNAGG 1 cut(s) 1503
EcoRV GATATC 1 cut(s) 349
EcoT22I ATGCAT 1 cut(s) 1119
EcoT38I GRGCYC 1 cut(s) 1013
FaeI CATG 7 cut(s) 854, 1133, 1157, 1178, 1222, 1316, 1400
FaqI GGGAC 2 cut(s) 274, 1097
FatI CATG 7 cut(s) 850, 1129, 1153, 1174, 1218, 1312, 1396
FbaI TGATCA 2 cut(s) 646, 927
FblI GTMKAC 1 cut(s) 1065
Fnu4HI GCNGC 4 cut(s) 159, 804, 1323, 1482
FokI GGATG 5 cut(s) 237, 580, 588, 1108, 1478
FriOI GRGCYC 1 cut(s) 1013
Fsp4HI GCNGC 4 cut(s) 159, 804, 1323, 1482
FspBI CTAG 1 cut(s) 1275
GlaI GCGC 1 cut(s) 1299
GluI GCNGC 4 cut(s) 159, 804, 1323, 1482
GsuI CTGGAG 1 cut(s) 55
HaeII RGCGCY 1 cut(s) 1301
HaeIII GGCC 1 cut(s) 1401
HapII CCGG 4 cut(s) 761, 920, 1158, 1359
HgaI GACGC 1 cut(s) 374
HhaI GCGC 1 cut(s) 1300
Hin1I GRCGYC 1 cut(s) 385
Hin1II CATG 7 cut(s) 854, 1133, 1157, 1178, 1222, 1316, 1400
Hin6I GCGC 1 cut(s) 1298
HinP1I GCGC 1 cut(s) 1298
HincII GTYRAC 1 cut(s) 1450
HindII GTYRAC 1 cut(s) 1450
HindIII AAGCTT 3 cut(s) 131, 377, 422
HinfI GANTC 4 cut(s) 173, 722, 1099, 1291
HpaII CCGG 4 cut(s) 761, 920, 1158, 1359
HphI GGTGA 3 cut(s) 344, 890, 923
Hpy166II GTNNAC 5 cut(s) 697, 939, 1066, 1394, 1450
Hpy188I TCNGA 6 cut(s) 651, 744, 787, 891, 1282, 1344
Hpy188III TCNNGA 8 cut(s) 60, 72, 302, 365, 428, 538, 611, 1142
Hpy8I GTNNAC 5 cut(s) 697, 939, 1066, 1394, 1450
HpyAV CCTTC 8 cut(s) 60, 252, 455, 535, 749, 1044, 1056, 1277
HpyCH4III ACNGT 4 cut(s) 14, 617, 830, 871
HpyCH4IV ACGT 2 cut(s) 1200, 1503
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 167, 812, 1322
HpyF3I CTNAG 2 cut(s) 45, 968
HpySE526I ACGT 2 cut(s) 1200, 1503
Hsp92I GRCGYC 1 cut(s) 385
Hsp92II CATG 7 cut(s) 854, 1133, 1157, 1178, 1222, 1316, 1400
HspAI GCGC 1 cut(s) 1298
Ksp22I TGATCA 2 cut(s) 646, 927
LmnI GCTCC 4 cut(s) 560, 864, 1189, 1509
Lsp1109I GCAGC 4 cut(s) 170, 815, 1309, 1493
LweI GCATC 6 cut(s) 31, 259, 543, 602, 663, 1104
MaeI CTAG 1 cut(s) 1275
MaeII ACGT 2 cut(s) 1200, 1503
MaeIII GTNAC 1 cut(s) 547
MboII GAAGA 8 cut(s) 45, 94, 133, 215, 293, 368, 893, 1123
MflI RGATCY 5 cut(s) 95, 220, 283, 748, 950
MhlI GDGCHC 1 cut(s) 1013
MlsI TGGCCA 1 cut(s) 1401
MluNI TGGCCA 1 cut(s) 1401
MlyI GAGTC 1 cut(s) 1093
MmeI TCCRAC 3 cut(s) 396, 413, 978
Mox20I TGGCCA 1 cut(s) 1401
Mph1103I ATGCAT 1 cut(s) 1119
MroXI GAANNNNTTC 1 cut(s) 360
MscI TGGCCA 1 cut(s) 1401
MseI TTAA 3 cut(s) 18, 315, 1052
MslI CAYNNNNRTG 2 cut(s) 1203, 1385
Msp20I TGGCCA 1 cut(s) 1401
MspI CCGG 4 cut(s) 761, 920, 1158, 1359
MspR9I CCNGG 3 cut(s) 761, 762, 920
Mva1269I GAATGC 1 cut(s) 817
MwoI GCNNNNNNNGC 4 cut(s) 158, 167, 812, 1322
NciI CCSGG 3 cut(s) 761, 762, 920
NlaIII CATG 7 cut(s) 854, 1133, 1157, 1178, 1222, 1316, 1400
NlaIV GGNNCC 3 cut(s) 750, 952, 1185
NmuCI GTSAC 1 cut(s) 547
NsiI ATGCAT 1 cut(s) 1119
PctI GAATGC 1 cut(s) 817
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 3 cut(s) 173, 722, 1291
PkrI GCNGC 4 cut(s) 160, 805, 1324, 1483
PleI GAGTC 1 cut(s) 1093
PpsI GAGTC 1 cut(s) 1093
PsiI TTATAA 1 cut(s) 519
PspN4I GGNNCC 3 cut(s) 750, 952, 1185
PstI CTGCAG 1 cut(s) 502
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 5 cut(s) 95, 220, 283, 748, 950
RsaI GTAC 3 cut(s) 206, 696, 940
RsaNI GTAC 3 cut(s) 205, 695, 939
RseI CAYNNNNRTG 2 cut(s) 1203, 1385
SaqAI TTAA 3 cut(s) 18, 315, 1052
SatI GCNGC 4 cut(s) 159, 804, 1323, 1482
SchI GAGTC 1 cut(s) 1093
ScrFI CCNGG 3 cut(s) 761, 762, 920
SduI GDGCHC 1 cut(s) 1013
SfaNI GCATC 6 cut(s) 31, 259, 543, 602, 663, 1104
SfcI CTRYAG 2 cut(s) 145, 498
SmaI CCCGGG 1 cut(s) 762
SmiMI CAYNNNNRTG 2 cut(s) 1203, 1385
SmlI CTYRAG 1 cut(s) 507
SmoI CTYRAG 1 cut(s) 507
SspMI CTAG 1 cut(s) 1275
StyD4I CCNGG 3 cut(s) 759, 760, 918
TaaI ACNGT 4 cut(s) 14, 617, 830, 871
TaiI ACGT 2 cut(s) 1203, 1506
TaqI TCGA 7 cut(s) 186, 364, 710, 755, 996, 1407, 1461
TaqII GACCGA 1 cut(s) 596
TatI WGTACW 1 cut(s) 938
TfiI GAWTC 3 cut(s) 173, 722, 1291
Tru1I TTAA 3 cut(s) 18, 315, 1052
Tru9I TTAA 3 cut(s) 18, 315, 1052
TscAI CASTG 5 cut(s) 17, 206, 502, 1387, 1491
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 4 cut(s) 158, 803, 1322, 1481
Tsp45I GTSAC 1 cut(s) 547
TspMI CCCGGG 1 cut(s) 760
TspRI CASTG 5 cut(s) 17, 206, 502, 1387, 1491
XagI CCTNNNNNAGG 1 cut(s) 1503
XapI RAATTY 2 cut(s) 124, 444
XmaI CCCGGG 1 cut(s) 760
XmiI GTMKAC 1 cut(s) 1065
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 1 cut(s) 1275
Zsp2I ATGCAT 1 cut(s) 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.