MD17G1071900.v1.1

tryptophan synthase beta chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0016013
Physical Location & Seq
Forward (+)
5778342 .. 5780420
2079 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1071900.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGAAGTTCGTGATGTATACAAGCTTTGGCGCCCATCACCTCTCATTAGGTTTGGCCAAGAGGTTGGAGAAGCTTCTTGACACACCTGCCAGAATTTACTACAAGTATGAAGGTGTCAGCCCGGCTGGATCACACAAGCCCAACTCTGCAGTTCCACAAGTGTGGTATAATGCACAAGAAGGAATCAAGAAGGTCGTGACAGAAACGGGAGCTGGCCAATGGGGAAGTGCATTGGCTTTTGCTTGCACCTTATTTGATGTTGAGTGTGAGGTGTGGCAAGTGCGTGCATCTTTTGATCAGAAACCATATCGCAGATTGATGATGCAAACTTGGGGTGCAAGGGTATACCCATCTCCATCCACCCTAACTGAAGCAGGTAGAAAAATCCTTCAAATGGATCCATCCAGCCCTGGAAGTTTGGGAATAGCTATTTCTGAAGCCGTGGAGATTGCAGCTATGAATGGTGATACTAAATACTGTCTAGGAAGTGTTCTCAACCATGTTTTGCTTCACCAGACAGTAATAGGAGAGGAGTGTCTAAAACAAATGGAGGCGATAGGTGAAGTCCCGGATGTTATCATAGGATGTACTGGTGGAGGATCCAATTTTGCAGGGCTCAGTTTCCCATTCATCAGGGAGAAGCTGAAAGGGAAAATGAACCCTGTAATTAGAGCAGTTGAACCTGCAGCATGTCCTTCGTTAACTAAAGGGGTATACGCATATGATTATGGCGATACAGCGGGGCTGACTCCACTAATGAAGATGCATACGCTTGGGCACGACTTCATCCCTGACCCAATTCATGCTGGAGGATTGCGCTACCATGGTATGTCACCGTTAATTTCACACGTCTACGAACTGGGTTACATGGAAGCAATTTCAATTCCCCAGATTGAGTGCTTTCAAGGTGCTATAAAATTTGCTAGAACCGAAGGATTGATACCAGCGCCAGAGCCAACACATGCCATAGCTGCCACCATCAGGGAAGCTCAGAAATGTCGAGAGACCGGAGAAGCCAAAGTGATTCTCACAGCAATGTGTGGGCATGGACATTTTGATTTGCCAGCTTACGAGAATTATCTGCAAGGAAATCTGGTGGATTTGTCATTTGAAGAGGAGAAGATTCAAGCATCATTGGCTAGTGTTCCTAAAGTAGGTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00400 ko01100 ko01110 ko01130 ko01230 map00260 map00400 map01100 map01110 map01130 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

388

Amino Acids

42.05

Weight (kDa)

6.23

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 19 - 348 1e-34 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016013)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38530
fragaria_vesca FvH4_6g45680
malus_domestica MD17G1071900.v1.1
prunus_persica Prupe.3G243000_v2.0.a1
pyrus_communis pycom17g07120
rosa_chinensis RchiOBHm_Chr2g0163831
rosa_laevigata RLG00000021419
rosa_multiflora Rmu_sc0005917.1_g000003
rosa_roxburghii Rroxscaffold_2G00086980
rosa_rugosa Rorug02G0504800
rosa_samantha Rh2AG572800 Rh2BG583500 Rh2CG554400 Rh2DG593100
rosa_wichuraiana Rw2G047360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 94
Acc36I ACCTGC 3 cut(s) 94, 365, 691
AccB1I GGYRCC 1 cut(s) 29
AccI GTMKAC 4 cut(s) 17, 345, 714, 852
AciI CCGC 1 cut(s) 740
AclWI GGATC 5 cut(s) 136, 392, 405, 594, 607
AcoI YGGCCR 2 cut(s) 54, 214
AcsI RAATTY 2 cut(s) 93, 917
AcuI CTGAAG 2 cut(s) 390, 456
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 1 cut(s) 589
AfiI CCNNNNNNNGG 3 cut(s) 394, 981, 1154
AflIII ACRYGT 1 cut(s) 847
AgsI TTSAA 6 cut(s) 392, 680, 882, 905, 1112, 1127
AjiI CACGTC 1 cut(s) 850
AjnI CCWGG 1 cut(s) 409
AleI CACNNNNGTG 1 cut(s) 160
AluBI AGCT 9 cut(s) 24, 73, 212, 428, 455, 643, 971, 989, 1067
AluI AGCT 9 cut(s) 24, 73, 212, 428, 455, 643, 971, 989, 1067
Alw26I GTCTC 1 cut(s) 998
AlwI GGATC 5 cut(s) 136, 392, 405, 594, 607
AoxI GGCC 2 cut(s) 54, 214
ApeKI GCWGC 3 cut(s) 452, 686, 971
ApoI RAATTY 2 cut(s) 93, 917
AspLEI GCGC 3 cut(s) 32, 819, 949
AsuC2I CCSGG 2 cut(s) 122, 569
AsuHPI GGTGA 5 cut(s) 29, 476, 503, 572, 825
BaeGI GKGCMC 1 cut(s) 780
BalI TGGCCA 2 cut(s) 56, 216
BamHI GGATCC 2 cut(s) 397, 599
BanI GGYRCC 1 cut(s) 29
BanII GRGCYC 1 cut(s) 618
BarI GAAGNNNNNNTAC 2 cut(s) 924, 956
BbvI GCAGC 3 cut(s) 464, 698, 958
BccI CCATC 5 cut(s) 42, 358, 364, 409, 986
BceAI ACGGC 1 cut(s) 425
BcgI CGANNNNNNTGC 2 cut(s) 678, 712
BciT130I CCWGG 1 cut(s) 411
BclI TGATCA 1 cut(s) 295
BcnI CCSGG 2 cut(s) 122, 569
BcoDI GTCTC 1 cut(s) 998
BfaI CTAG 3 cut(s) 482, 924, 1140
BfmI CTRYAG 2 cut(s) 147, 684
BfoI RGCGCY 2 cut(s) 33, 950
BfuAI ACCTGC 3 cut(s) 94, 365, 691
BisI GCNGC 3 cut(s) 453, 687, 972
BlsI GCNGC 3 cut(s) 454, 688, 973
Bme1390I CCNGG 3 cut(s) 122, 411, 569
BmgBI CACGTC 1 cut(s) 850
BmiI GGNNCC 3 cut(s) 31, 399, 601
BmrFI CCNGG 3 cut(s) 122, 411, 569
BmrI ACTGGG 1 cut(s) 869
BmsI GCATC 4 cut(s) 296, 312, 753, 1139
BmuI ACTGGG 1 cut(s) 869
BpmI CTGGAG 1 cut(s) 828
BpuMI CCSGG 2 cut(s) 122, 569
BsaHI GRCGYC 1 cut(s) 30
BsaI GGTCTC 1 cut(s) 998
BsaJI CCNNGG 3 cut(s) 409, 441, 823
BsaWI WCCGGW 1 cut(s) 1007
Bsc4I CCNNNNNNNGG 3 cut(s) 394, 981, 1154
Bse1I ACTGG 2 cut(s) 595, 864
Bse3DI GCAATG 1 cut(s) 1041
BseBI CCWGG 1 cut(s) 411
BseDI CCNNGG 3 cut(s) 409, 441, 823
BseGI GGATG 5 cut(s) 356, 401, 577, 590, 786
BseLI CCNNNNNNNGG 3 cut(s) 394, 981, 1154
BseMI GCAATG 1 cut(s) 1041
BseMII CTCAG 2 cut(s) 631, 1004
BseNI ACTGG 2 cut(s) 595, 864
BseRI GAGGAG 2 cut(s) 545, 1130
BseSI GKGCMC 1 cut(s) 780
BseXI GCAGC 3 cut(s) 464, 698, 958
BshFI GGCC 2 cut(s) 56, 216
BshNI GGYRCC 1 cut(s) 29
BsiSI CCGG 3 cut(s) 122, 569, 1008
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 3 cut(s) 394, 981, 1154
BsmAI GTCTC 1 cut(s) 998
BsmFI GGGAC 1 cut(s) 551
BsnI GGCC 2 cut(s) 56, 216
Bso31I GGTCTC 1 cut(s) 998
Bsp1286I GDGCHC 2 cut(s) 618, 780
Bsp143I GATC 4 cut(s) 128, 295, 397, 599
Bsp19I CCATGG 1 cut(s) 823
BspACI CCGC 1 cut(s) 740
BspANI GGCC 2 cut(s) 56, 216
BspCNI CTCAG 2 cut(s) 630, 1003
BspLI GGNNCC 3 cut(s) 31, 399, 601
BspMAI CTGCAG 2 cut(s) 151, 688
BspMI ACCTGC 3 cut(s) 94, 365, 691
BspPI GGATC 5 cut(s) 136, 392, 405, 594, 607
BspT107I GGYRCC 1 cut(s) 29
BspTNI GGTCTC 1 cut(s) 998
BsrDI GCAATG 1 cut(s) 1041
BsrI ACTGG 2 cut(s) 595, 864
BssECI CCNNGG 3 cut(s) 409, 441, 823
BssMI GATC 4 cut(s) 128, 295, 397, 599
BssNAI GTATAC 3 cut(s) 18, 346, 715
BssNI GRCGYC 1 cut(s) 30
BssT1I CCWWGG 1 cut(s) 823
Bst1107I GTATAC 3 cut(s) 18, 346, 715
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 3 cut(s) 479, 520, 837
Bst6I CTCTTC 1 cut(s) 1107
BstACI GRCGYC 1 cut(s) 30
BstC8I GCNNGC 4 cut(s) 214, 244, 285, 1065
BstDEI CTNAG 2 cut(s) 617, 990
BstDSI CCRYGG 2 cut(s) 441, 823
BstENI CCTNNNNNAGG 1 cut(s) 1152
BstF5I GGATG 5 cut(s) 356, 401, 577, 590, 786
BstH2I RGCGCY 2 cut(s) 33, 950
BstHHI GCGC 3 cut(s) 32, 819, 949
BstKTI GATC 4 cut(s) 131, 298, 400, 602
BstMAI GTCTC 1 cut(s) 998
BstMBI GATC 4 cut(s) 128, 295, 397, 599
BstMWI GCNNNNNNNGC 2 cut(s) 971, 1136
BstNI CCWGG 1 cut(s) 411
BstNSI RCATGY 2 cut(s) 693, 965
BstSCI CCNGG 3 cut(s) 120, 409, 567
BstSFI CTRYAG 2 cut(s) 147, 684
BstSLI GKGCMC 1 cut(s) 780
BstV1I GCAGC 3 cut(s) 464, 698, 958
BstX2I RGATCY 2 cut(s) 397, 599
BstXI CCANNNNNNTGG 2 cut(s) 64, 162
BstYI RGATCY 2 cut(s) 397, 599
BstZ17I GTATAC 3 cut(s) 18, 346, 715
BsuRI GGCC 2 cut(s) 56, 216
BtgI CCRYGG 2 cut(s) 441, 823
BtrI CACGTC 1 cut(s) 850
BtsCI GGATG 5 cut(s) 356, 401, 577, 590, 786
BveI ACCTGC 3 cut(s) 94, 365, 691
Cac8I GCNNGC 4 cut(s) 214, 244, 285, 1065
CfoI GCGC 3 cut(s) 32, 819, 949
Csp6I GTAC 1 cut(s) 588
CviAII CATG 7 cut(s) 500, 690, 803, 824, 868, 962, 1046
CviQI GTAC 1 cut(s) 588
DdeI CTNAG 2 cut(s) 617, 990
DinI GGCGCC 1 cut(s) 31
DpnI GATC 4 cut(s) 130, 297, 399, 601
DpnII GATC 4 cut(s) 128, 295, 397, 599
EaeI YGGCCR 2 cut(s) 54, 214
Eam1104I CTCTTC 1 cut(s) 1107
EarI CTCTTC 1 cut(s) 1107
Eco130I CCWWGG 1 cut(s) 823
Eco24I GRGCYC 1 cut(s) 618
Eco31I GGTCTC 1 cut(s) 998
Eco57I CTGAAG 2 cut(s) 390, 456
EcoNI CCTNNNNNAGG 1 cut(s) 1152
EcoRII CCWGG 1 cut(s) 409
EcoT14I CCWWGG 1 cut(s) 823
EcoT22I ATGCAT 1 cut(s) 768
EcoT38I GRGCYC 1 cut(s) 618
EgeI GGCGCC 1 cut(s) 31
EheI GGCGCC 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 823
FaeI CATG 7 cut(s) 503, 693, 806, 827, 871, 965, 1049
FaqI GGGAC 1 cut(s) 551
FatI CATG 7 cut(s) 499, 689, 802, 823, 867, 961, 1045
FauI CCCGC 1 cut(s) 733
FauNDI CATATG 1 cut(s) 721
FbaI TGATCA 1 cut(s) 295
FblI GTMKAC 4 cut(s) 17, 345, 714, 852
Fnu4HI GCNGC 3 cut(s) 453, 687, 972
FokI GGATG 5 cut(s) 343, 388, 584, 597, 773
FriOI GRGCYC 1 cut(s) 618
Fsp4HI GCNGC 3 cut(s) 453, 687, 972
FspBI CTAG 3 cut(s) 482, 924, 1140
GlaI GCGC 3 cut(s) 31, 818, 948
GluI GCNGC 3 cut(s) 453, 687, 972
GsuI CTGGAG 1 cut(s) 828
HaeII RGCGCY 2 cut(s) 33, 950
HaeIII GGCC 2 cut(s) 56, 216
HapII CCGG 3 cut(s) 122, 569, 1008
HhaI GCGC 3 cut(s) 32, 819, 949
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 7 cut(s) 503, 693, 806, 827, 871, 965, 1049
Hin6I GCGC 3 cut(s) 30, 817, 947
HinP1I GCGC 3 cut(s) 30, 817, 947
HincII GTYRAC 1 cut(s) 702
HindII GTYRAC 1 cut(s) 702
HindIII AAGCTT 2 cut(s) 22, 71
HinfI GANTC 4 cut(s) 183, 748, 1024, 1123
HpaI GTTAAC 1 cut(s) 702
HpaII CCGG 3 cut(s) 122, 569, 1008
HphI GGTGA 5 cut(s) 29, 476, 503, 572, 825
Hpy166II GTNNAC 5 cut(s) 18, 346, 702, 715, 853
Hpy188I TCNGA 3 cut(s) 300, 436, 993
Hpy188III TCNNGA 5 cut(s) 10, 77, 187, 196, 1001
Hpy8I GTNNAC 5 cut(s) 18, 346, 702, 715, 853
HpyAV CCTTC 6 cut(s) 104, 173, 184, 398, 705, 926
HpyCH4III ACNGT 3 cut(s) 479, 520, 837
HpyCH4IV ACGT 1 cut(s) 849
HpyF10VI GCNNNNNNNGC 2 cut(s) 971, 1136
HpyF3I CTNAG 2 cut(s) 617, 990
HpySE526I ACGT 1 cut(s) 849
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 7 cut(s) 503, 693, 806, 827, 871, 965, 1049
HspAI GCGC 3 cut(s) 30, 817, 947
KasI GGCGCC 1 cut(s) 29
Ksp22I TGATCA 1 cut(s) 295
KspAI GTTAAC 1 cut(s) 702
Kzo9I GATC 4 cut(s) 128, 295, 397, 599
LmnI GCTCC 1 cut(s) 209
Lsp1109I GCAGC 3 cut(s) 464, 698, 958
LweI GCATC 4 cut(s) 296, 312, 753, 1139
MaeI CTAG 3 cut(s) 482, 924, 1140
MaeII ACGT 1 cut(s) 849
MaeIII GTNAC 3 cut(s) 196, 831, 863
MalI GATC 4 cut(s) 130, 297, 399, 601
MboI GATC 4 cut(s) 128, 295, 397, 599
MboII GAAGA 3 cut(s) 772, 1124, 1132
MflI RGATCY 2 cut(s) 397, 599
MhlI GDGCHC 2 cut(s) 618, 780
MlsI TGGCCA 2 cut(s) 56, 216
MluCI AATT 9 cut(s) 93, 604, 666, 798, 840, 876, 882, 917, 1075
MluNI TGGCCA 2 cut(s) 56, 216
Mly113I GGCGCC 1 cut(s) 30
MlyI GAGTC 1 cut(s) 742
MmeI TCCRAC 1 cut(s) 45
MnlI CCTC 8 cut(s) 50, 54, 262, 523, 544, 590, 803, 1108
Mox20I TGGCCA 2 cut(s) 56, 216
Mph1103I ATGCAT 1 cut(s) 768
MscI TGGCCA 2 cut(s) 56, 216
MseI TTAA 2 cut(s) 701, 839
MslI CAYNNNNRTG 2 cut(s) 160, 1034
Msp20I TGGCCA 2 cut(s) 56, 216
MspA1I CMGCKG 1 cut(s) 740
MspI CCGG 3 cut(s) 122, 569, 1008
MspR9I CCNGG 3 cut(s) 122, 411, 569
MvaI CCWGG 1 cut(s) 411
MwoI GCNNNNNNNGC 2 cut(s) 971, 1136
NarI GGCGCC 1 cut(s) 30
NciI CCSGG 2 cut(s) 122, 569
NcoI CCATGG 1 cut(s) 823
NdeI CATATG 1 cut(s) 721
NdeII GATC 4 cut(s) 128, 295, 397, 599
NlaIII CATG 7 cut(s) 503, 693, 806, 827, 871, 965, 1049
NlaIV GGNNCC 3 cut(s) 31, 399, 601
NmuCI GTSAC 2 cut(s) 196, 831
NsiI ATGCAT 1 cut(s) 768
NspI RCATGY 2 cut(s) 693, 965
OliI CACNNNNGTG 1 cut(s) 160
PaqCI CACCTGC 1 cut(s) 94
PfeI GAWTC 3 cut(s) 183, 1024, 1123
PfoI TCCNGGA 1 cut(s) 567
PkrI GCNGC 3 cut(s) 454, 688, 973
PleI GAGTC 1 cut(s) 742
PluTI GGCGCC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 742
Psp6I CCWGG 1 cut(s) 409
PspGI CCWGG 1 cut(s) 409
PspN4I GGNNCC 3 cut(s) 31, 399, 601
PstI CTGCAG 2 cut(s) 151, 688
PsuI RGATCY 2 cut(s) 397, 599
RsaI GTAC 1 cut(s) 589
RsaNI GTAC 1 cut(s) 588
RseI CAYNNNNRTG 2 cut(s) 160, 1034
SaqAI TTAA 2 cut(s) 701, 839
SatI GCNGC 3 cut(s) 453, 687, 972
Sau3AI GATC 4 cut(s) 128, 295, 397, 599
SchI GAGTC 1 cut(s) 742
ScrFI CCNGG 3 cut(s) 122, 411, 569
SduI GDGCHC 2 cut(s) 618, 780
SfaNI GCATC 4 cut(s) 296, 312, 753, 1139
SfcI CTRYAG 2 cut(s) 147, 684
SfoI GGCGCC 1 cut(s) 31
SmiMI CAYNNNNRTG 2 cut(s) 160, 1034
Sse9I AATT 9 cut(s) 93, 604, 666, 798, 840, 876, 882, 917, 1075
SsiI CCGC 1 cut(s) 740
SspDI GGCGCC 1 cut(s) 29
SspMI CTAG 3 cut(s) 482, 924, 1140
StyD4I CCNGG 3 cut(s) 120, 409, 567
StyI CCWWGG 1 cut(s) 823
TaaI ACNGT 3 cut(s) 479, 520, 837
TaiI ACGT 1 cut(s) 852
TaqI TCGA 1 cut(s) 1000
TasI AATT 9 cut(s) 93, 604, 666, 798, 840, 876, 882, 917, 1075
TatI WGTACW 1 cut(s) 587
TfiI GAWTC 3 cut(s) 183, 1024, 1123
Tru1I TTAA 2 cut(s) 701, 839
Tru9I TTAA 2 cut(s) 701, 839
TseFI GTSAC 2 cut(s) 196, 831
TseI GCWGC 3 cut(s) 452, 686, 971
Tsp45I GTSAC 2 cut(s) 196, 831
TspDTI ATGAA 8 cut(s) 17, 123, 473, 619, 671, 773, 775, 791
XagI CCTNNNNNAGG 1 cut(s) 1152
XapI RAATTY 2 cut(s) 93, 917
XceI RCATGY 2 cut(s) 693, 965
XmiI GTMKAC 4 cut(s) 17, 345, 714, 852
XspI CTAG 3 cut(s) 482, 924, 1140
Zsp2I ATGCAT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.