RLG00000021419

tryptophan synthase beta chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016013
Physical Location & Seq
Reverse (-)
75244811 .. 75248770
3960 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021419

Sequence Viewer

Length: 1572 bp
ATGGCAACTCACTGTGTTTTTAATTTCTGTGCAAGTTCATTTGATGCTAAGCCGCCTTCATCAGAATCACACTCCAGAGTTGAGAAGAAATGGCTTGGATCTATTGCACTTGATAGAAAGCTGAAGAATTTGAAGCTTTCAAATGGCTGTAGAGCAAGAGCAGCCCTCAATGCTGATTCAATACCAATCGAAATCCCTCGTCAGTGGTACAACCTGATTGCAGATCTTCCTATAAAACCTCCTCCTCCATTGCATCCCAAGACTTTTGAACCAGTAAAACCTGAAGATCTGTCCCCTCTATTTCCTGATGAGTTGATTAAACAGGAAGCAAGTGGTGAAAGATACATAGATATCCCAGAAGAAGTTCGAGAGATATACAAGCTTTGGCGTCCAACCCCTCTCATTAGGGCAAAGAGGTTGGAGAAGCTTCTTGACACGCCTGCCAGAATTTATTACAAGTATGAAGGTGTTAGCCCTGCTGGATCACACAAACCCAACACTGCAGTTCCTCAGGTGTATTATAATGCACAACAAGGCATCAAGAAGGTTGTGACAGAAACAGGAGCTGGTCAATGGGGATGTTCTTTGGCTTTTGCATCCAGCTTATTCGGTCTTGAATGTGAGGTATGGCAAGTGCGCGCTTCGTATGATCAGAAACCATATCGCAAATTGATGATGCAAACTTGGGGGGCAAAGGTACACCCATCTCCATCGAACATAACTGAATCAGGTAGAAAAATCCTTCTGATGGATCCATCGACCCCGGGAAGTTTAGGAATAGCTATATCTGAAGCTGTGGAGGTTGCAGCTATGAATACTGACACTAAATACTGTCTGGGAAGTGTTCTCAATCATGTTTTGCTCCACCAGACTGTAATAGGTGAAGAGTGTCTGAAACAAATGGAAGCGATAGGTGAAACCCCGGACTTGATCATAGGGTGTACAGGTGGAGGATCCAACTTTGCAGGGCTAAGTTTCCCATTCATTAGAGAAAAGCTCGATGGGAAAATGAGCCCTGTAATTAGAGCAGTTGAGCCTTCTGCTTGTCCTTCATTAACTAAAGGTGTATACAGATATGATTATGGTGACACAGCAGGGATGACTCCACTAATGAAGATGCATACACTTGGGCATGACTTCATTCCGGACCCAATTCATGCCGGAGGATTGCGTTATCATGGAATGGCTCCATTGATTTCACACGTCTATGAACTCGGTTTCATGGAAGCACTTTCAATTCCCCAGACTGAGTGCTTCAAAGGTGCTATACAATTTGCTAGGTCCGAAGGATTGATTCCTGCGCCAGAGCCAACCCATGCCATAGCTGCCACCATTAGGGAGGCTCTGAAGTGTAAAGAGACCGGAGAAGCAAAAGTTATTCTCACTGCAATGTGTGGACATGGCCATTTCGATTTGCCAGCTTATGACAATTATCTGCAAGGAAATCTGGTTGACCTGTCATTCGAGGATGAGAAGCTACAAGCAGCACTGGGTAGTGTTCCTAACATCACACAGTCACAGAGAACGGAGGTCCTTCAAATTGTTCTTCAGAGAAACATTCATAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00400 ko01100 ko01110 ko01130 ko01230 map00260 map00400 map01100 map01110 map01130 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

524

Amino Acids

57.72

Weight (kDa)

6.96

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 128 - 466 2.5e-38 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016013)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38530
fragaria_vesca FvH4_6g45680
malus_domestica MD17G1071900.v1.1
prunus_persica Prupe.3G243000_v2.0.a1
pyrus_communis pycom17g07120
rosa_chinensis RchiOBHm_Chr2g0163831
rosa_laevigata RLG00000021419
rosa_multiflora Rmu_sc0005917.1_g000003
rosa_roxburghii Rroxscaffold_2G00086980
rosa_rugosa Rorug02G0504800
rosa_samantha Rh2AG572800 Rh2BG583500 Rh2CG554400 Rh2DG593100
rosa_wichuraiana Rw2G047360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 522
AccI GTMKAC 1 cut(s) 1068
AccII CGCG 1 cut(s) 639
AccIII TCCGGA 1 cut(s) 1144
AciI CCGC 1 cut(s) 53
AclWI GGATC 6 cut(s) 106, 490, 746, 759, 948, 961
AcoI YGGCCR 1 cut(s) 1402
AcsI RAATTY 2 cut(s) 127, 447
AcuI CTGAAG 5 cut(s) 143, 303, 810, 1367, 1532
AcyI GRCGYC 1 cut(s) 388
AfaI GTAC 3 cut(s) 209, 699, 943
AfiI CCNNNNNNNGG 2 cut(s) 748, 1335
AflIII ACRYGT 1 cut(s) 1201
AgsI TTSAA 8 cut(s) 133, 141, 180, 269, 617, 1236, 1258, 1538
AjiI CACGTC 1 cut(s) 1204
Alw26I GTCTC 1 cut(s) 1352
AlwI GGATC 6 cut(s) 106, 490, 746, 759, 948, 961
AlwNI CAGNNNCTG 1 cut(s) 566
Ama87I CYCGRG 1 cut(s) 763
Aor13HI TCCGGA 1 cut(s) 1144
AoxI GGCC 1 cut(s) 1402
ApeKI GCWGC 4 cut(s) 161, 806, 1325, 1484
ApoI RAATTY 2 cut(s) 127, 447
ArsI GACNNNNNNTTYG 2 cut(s) 1445, 1477
Asp700I GAANNNNTTC 1 cut(s) 363
AspLEI GCGC 3 cut(s) 639, 641, 1303
AspS9I GGNCC 3 cut(s) 1147, 1281, 1531
AsuC2I CCSGG 3 cut(s) 764, 765, 923
AsuHPI GGTGA 4 cut(s) 347, 893, 926, 1097
AvaI CYCGRG 1 cut(s) 763
AvaII GGWCC 3 cut(s) 1147, 1281, 1531
AxyI CCTNAGG 1 cut(s) 510
BalI TGGCCA 1 cut(s) 1404
BamHI GGATCC 2 cut(s) 751, 953
BanII GRGCYC 1 cut(s) 1016
BbvI GCAGC 4 cut(s) 173, 818, 1312, 1496
BccI CCATC 5 cut(s) 712, 718, 742, 763, 995
BclI TGATCA 2 cut(s) 649, 930
BcnI CCSGG 3 cut(s) 764, 765, 923
BcoDI GTCTC 1 cut(s) 1352
BfaI CTAG 1 cut(s) 1278
BfmI CTRYAG 2 cut(s) 148, 501
BglII AGATCT 2 cut(s) 223, 286
BisI GCNGC 5 cut(s) 53, 162, 807, 1326, 1485
BlpI GCTNAGC 1 cut(s) 48
BlsI GCNGC 5 cut(s) 54, 163, 808, 1327, 1486
Bme1390I CCNGG 3 cut(s) 764, 765, 923
Bme18I GGWCC 3 cut(s) 1147, 1281, 1531
BmeT110I CYCGRG 1 cut(s) 763
BmgBI CACGTC 1 cut(s) 1204
BmgT120I GGNCC 3 cut(s) 1147, 1281, 1531
BmiI GGNNCC 4 cut(s) 753, 955, 1149, 1188
BmrFI CCNGG 3 cut(s) 764, 765, 923
BmrI ACTGGG 1 cut(s) 1499
BmsI GCATC 6 cut(s) 34, 262, 546, 605, 666, 1107
BmuI ACTGGG 1 cut(s) 1499
BplI GAGNNNNNCTC 4 cut(s) 150, 182, 981, 1013
BpmI CTGGAG 1 cut(s) 58
Bpu1102I GCTNAGC 1 cut(s) 48
BpuMI CCSGG 3 cut(s) 764, 765, 923
BsaHI GRCGYC 1 cut(s) 388
BsaI GGTCTC 1 cut(s) 1352
BsaJI CCNNGG 3 cut(s) 762, 763, 921
BsaWI WCCGGW 2 cut(s) 1144, 1361
Bsc4I CCNNNNNNNGG 2 cut(s) 748, 1335
Bse1I ACTGG 2 cut(s) 272, 1494
Bse21I CCTNAGG 1 cut(s) 510
Bse3DI GCAATG 2 cut(s) 248, 1395
BseAI TCCGGA 1 cut(s) 1144
BseDI CCNNGG 3 cut(s) 762, 763, 921
BseGI GGATG 5 cut(s) 253, 584, 596, 1104, 1474
BseLI CCNNNNNNNGG 2 cut(s) 748, 1335
BseMI GCAATG 2 cut(s) 248, 1395
BseMII CTCAG 2 cut(s) 524, 1239
BseNI ACTGG 2 cut(s) 272, 1494
BsePI GCGCGC 1 cut(s) 637
BseRI GAGGAG 2 cut(s) 231, 234
BseXI GCAGC 4 cut(s) 173, 818, 1312, 1496
Bsh1236I CGCG 1 cut(s) 639
BshFI GGCC 1 cut(s) 1404
BsiHKCI CYCGRG 1 cut(s) 763
BsiSI CCGG 5 cut(s) 764, 923, 1145, 1161, 1362
BslFI GGGAC 1 cut(s) 277
BslI CCNNNNNNNGG 2 cut(s) 748, 1335
BsmAI GTCTC 1 cut(s) 1352
BsmFI GGGAC 1 cut(s) 277
BsnI GGCC 1 cut(s) 1404
Bso31I GGTCTC 1 cut(s) 1352
BsoBI CYCGRG 1 cut(s) 763
Bsp1286I GDGCHC 1 cut(s) 1016
Bsp13I TCCGGA 1 cut(s) 1144
Bsp1407I TGTACA 1 cut(s) 941
Bsp143I GATC 8 cut(s) 98, 223, 286, 482, 649, 751, 930, 953
Bsp1720I GCTNAGC 1 cut(s) 48
BspACI CCGC 1 cut(s) 53
BspANI GGCC 1 cut(s) 1404
BspCNI CTCAG 2 cut(s) 523, 1240
BspEI TCCGGA 1 cut(s) 1144
BspFNI CGCG 1 cut(s) 639
BspLI GGNNCC 4 cut(s) 753, 955, 1149, 1188
BspMAI CTGCAG 1 cut(s) 505
BspPI GGATC 6 cut(s) 106, 490, 746, 759, 948, 961
BspTNI GGTCTC 1 cut(s) 1352
BsrDI GCAATG 2 cut(s) 248, 1395
BsrGI TGTACA 1 cut(s) 941
BsrI ACTGG 2 cut(s) 272, 1494
BssECI CCNNGG 3 cut(s) 762, 763, 921
BssHII GCGCGC 1 cut(s) 637
BssMI GATC 8 cut(s) 98, 223, 286, 482, 649, 751, 930, 953
BssNAI GTATAC 1 cut(s) 1069
BssNI GRCGYC 1 cut(s) 388
Bst1107I GTATAC 1 cut(s) 1069
Bst4CI ACNGT 4 cut(s) 14, 833, 874, 1515
Bst6I CTCTTC 1 cut(s) 879
BstACI GRCGYC 1 cut(s) 388
BstAUI TGTACA 1 cut(s) 941
BstC8I GCNNGC 3 cut(s) 441, 639, 1419
BstDEI CTNAG 4 cut(s) 48, 510, 971, 1248
BstF5I GGATG 5 cut(s) 253, 584, 596, 1104, 1474
BstFNI CGCG 1 cut(s) 639
BstHHI GCGC 3 cut(s) 639, 641, 1303
BstKTI GATC 8 cut(s) 101, 226, 289, 485, 652, 754, 933, 956
BstMAI GTCTC 1 cut(s) 1352
BstMBI GATC 8 cut(s) 98, 223, 286, 482, 649, 751, 930, 953
BstMWI GCNNNNNNNGC 3 cut(s) 161, 170, 1325
BstSCI CCNGG 3 cut(s) 762, 763, 921
BstSFI CTRYAG 2 cut(s) 148, 501
BstUI CGCG 1 cut(s) 639
BstV1I GCAGC 4 cut(s) 173, 818, 1312, 1496
BstX2I RGATCY 5 cut(s) 98, 223, 286, 751, 953
BstYI RGATCY 5 cut(s) 98, 223, 286, 751, 953
BstZ17I GTATAC 1 cut(s) 1069
Bsu36I CCTNAGG 1 cut(s) 510
BsuRI GGCC 1 cut(s) 1404
BtrI CACGTC 1 cut(s) 1204
BtsCI GGATG 5 cut(s) 253, 584, 596, 1104, 1474
BtsI GCAGTG 2 cut(s) 498, 1383
BtsIMutI CAGTG 5 cut(s) 10, 209, 498, 1383, 1487
Cac8I GCNNGC 3 cut(s) 441, 639, 1419
CaiI CAGNNNCTG 1 cut(s) 566
CfoI GCGC 3 cut(s) 639, 641, 1303
Cfr13I GGNCC 3 cut(s) 1147, 1281, 1531
Cfr9I CCCGGG 1 cut(s) 763
CseI GACGC 1 cut(s) 377
Csp6I GTAC 3 cut(s) 208, 698, 942
CviAII CATG 7 cut(s) 854, 1133, 1157, 1178, 1222, 1316, 1400
CviQI GTAC 3 cut(s) 208, 698, 942
DdeI CTNAG 4 cut(s) 48, 510, 971, 1248
DpnI GATC 8 cut(s) 100, 225, 288, 484, 651, 753, 932, 955
DpnII GATC 8 cut(s) 98, 223, 286, 482, 649, 751, 930, 953
EaeI YGGCCR 1 cut(s) 1402
Eam1104I CTCTTC 1 cut(s) 879
EarI CTCTTC 1 cut(s) 879
Eco24I GRGCYC 1 cut(s) 1016
Eco31I GGTCTC 1 cut(s) 1352
Eco32I GATATC 1 cut(s) 352
Eco47I GGWCC 3 cut(s) 1147, 1281, 1531
Eco57I CTGAAG 5 cut(s) 143, 303, 810, 1367, 1532
Eco81I CCTNAGG 1 cut(s) 510
Eco88I CYCGRG 1 cut(s) 763
EcoO109I RGGNCCY 1 cut(s) 1531
EcoRV GATATC 1 cut(s) 352
EcoT22I ATGCAT 1 cut(s) 1122
EcoT38I GRGCYC 1 cut(s) 1016
FaeI CATG 7 cut(s) 857, 1136, 1160, 1181, 1225, 1319, 1403
FaqI GGGAC 1 cut(s) 277
FatI CATG 7 cut(s) 853, 1132, 1156, 1177, 1221, 1315, 1399
FbaI TGATCA 2 cut(s) 649, 930
FblI GTMKAC 1 cut(s) 1068
Fnu4HI GCNGC 5 cut(s) 53, 162, 807, 1326, 1485
FokI GGATG 5 cut(s) 240, 583, 591, 1111, 1481
FriOI GRGCYC 1 cut(s) 1016
Fsp4HI GCNGC 5 cut(s) 53, 162, 807, 1326, 1485
FspBI CTAG 1 cut(s) 1278
GlaI GCGC 3 cut(s) 638, 640, 1302
GluI GCNGC 5 cut(s) 53, 162, 807, 1326, 1485
GsuI CTGGAG 1 cut(s) 58
HaeIII GGCC 1 cut(s) 1404
HapII CCGG 5 cut(s) 764, 923, 1145, 1161, 1362
HgaI GACGC 1 cut(s) 377
HhaI GCGC 3 cut(s) 639, 641, 1303
Hin1I GRCGYC 1 cut(s) 388
Hin1II CATG 7 cut(s) 857, 1136, 1160, 1181, 1225, 1319, 1403
Hin6I GCGC 3 cut(s) 637, 639, 1301
HinP1I GCGC 3 cut(s) 637, 639, 1301
HincII GTYRAC 1 cut(s) 1453
HindII GTYRAC 1 cut(s) 1453
HindIII AAGCTT 3 cut(s) 134, 380, 425
HinfI GANTC 5 cut(s) 65, 176, 725, 1102, 1294
HpaII CCGG 5 cut(s) 764, 923, 1145, 1161, 1362
HphI GGTGA 4 cut(s) 347, 893, 926, 1097
Hpy166II GTNNAC 5 cut(s) 700, 942, 1069, 1397, 1453
Hpy188I TCNGA 8 cut(s) 64, 654, 747, 790, 894, 1285, 1347, 1551
Hpy188III TCNNGA 7 cut(s) 75, 305, 368, 431, 541, 614, 1145
Hpy8I GTNNAC 5 cut(s) 700, 942, 1069, 1397, 1453
HpyAV CCTTC 8 cut(s) 66, 458, 538, 752, 1047, 1059, 1280, 1544
HpyCH4III ACNGT 4 cut(s) 14, 833, 874, 1515
HpyCH4IV ACGT 1 cut(s) 1203
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 170, 1325
HpyF3I CTNAG 4 cut(s) 48, 510, 971, 1248
HpySE526I ACGT 1 cut(s) 1203
Hsp92I GRCGYC 1 cut(s) 388
Hsp92II CATG 7 cut(s) 857, 1136, 1160, 1181, 1225, 1319, 1403
HspAI GCGC 3 cut(s) 637, 639, 1301
Kpn2I TCCGGA 1 cut(s) 1144
Ksp22I TGATCA 2 cut(s) 649, 930
Kzo9I GATC 8 cut(s) 98, 223, 286, 482, 649, 751, 930, 953
LmnI GCTCC 3 cut(s) 563, 867, 1192
Lsp1109I GCAGC 4 cut(s) 173, 818, 1312, 1496
LweI GCATC 6 cut(s) 34, 262, 546, 605, 666, 1107
MaeI CTAG 1 cut(s) 1278
MaeII ACGT 1 cut(s) 1203
MaeIII GTNAC 3 cut(s) 550, 1085, 1515
MalI GATC 8 cut(s) 100, 225, 288, 484, 651, 753, 932, 955
MboI GATC 8 cut(s) 98, 223, 286, 482, 649, 751, 930, 953
MboII GAAGA 8 cut(s) 97, 136, 218, 296, 371, 896, 1126, 1538
MflI RGATCY 5 cut(s) 98, 223, 286, 751, 953
MhlI GDGCHC 1 cut(s) 1016
MlsI TGGCCA 1 cut(s) 1404
MluNI TGGCCA 1 cut(s) 1404
MlyI GAGTC 1 cut(s) 1096
MmeI TCCRAC 3 cut(s) 399, 416, 981
Mox20I TGGCCA 1 cut(s) 1404
Mph1103I ATGCAT 1 cut(s) 1122
MroI TCCGGA 1 cut(s) 1144
MroXI GAANNNNTTC 1 cut(s) 363
MscI TGGCCA 1 cut(s) 1404
MseI TTAA 3 cut(s) 21, 318, 1055
MslI CAYNNNNRTG 2 cut(s) 1206, 1388
Msp20I TGGCCA 1 cut(s) 1404
MspI CCGG 5 cut(s) 764, 923, 1145, 1161, 1362
MspR9I CCNGG 3 cut(s) 764, 765, 923
MvnI CGCG 1 cut(s) 639
MwoI GCNNNNNNNGC 3 cut(s) 161, 170, 1325
NciI CCSGG 3 cut(s) 764, 765, 923
NdeII GATC 8 cut(s) 98, 223, 286, 482, 649, 751, 930, 953
NlaIII CATG 7 cut(s) 857, 1136, 1160, 1181, 1225, 1319, 1403
NlaIV GGNNCC 4 cut(s) 753, 955, 1149, 1188
NmuCI GTSAC 3 cut(s) 550, 1085, 1515
NsiI ATGCAT 1 cut(s) 1122
PauI GCGCGC 1 cut(s) 637
PdmI GAANNNNTTC 1 cut(s) 363
PfeI GAWTC 4 cut(s) 65, 176, 725, 1294
PkrI GCNGC 5 cut(s) 54, 163, 808, 1327, 1486
PleI GAGTC 1 cut(s) 1096
PpsI GAGTC 1 cut(s) 1096
PpuMI RGGWCCY 1 cut(s) 1531
PsiI TTATAA 1 cut(s) 522
Psp5II RGGWCCY 1 cut(s) 1531
PspN4I GGNNCC 4 cut(s) 753, 955, 1149, 1188
PspPI GGNCC 3 cut(s) 1147, 1281, 1531
PspPPI RGGWCCY 1 cut(s) 1531
PstI CTGCAG 1 cut(s) 505
PstNI CAGNNNCTG 1 cut(s) 566
PsuI RGATCY 5 cut(s) 98, 223, 286, 751, 953
PteI GCGCGC 1 cut(s) 637
RsaI GTAC 3 cut(s) 209, 699, 943
RsaNI GTAC 3 cut(s) 208, 698, 942
RseI CAYNNNNRTG 2 cut(s) 1206, 1388
SaqAI TTAA 3 cut(s) 21, 318, 1055
SatI GCNGC 5 cut(s) 53, 162, 807, 1326, 1485
Sau3AI GATC 8 cut(s) 98, 223, 286, 482, 649, 751, 930, 953
Sau96I GGNCC 3 cut(s) 1147, 1281, 1531
SchI GAGTC 1 cut(s) 1096
ScrFI CCNGG 3 cut(s) 764, 765, 923
SduI GDGCHC 1 cut(s) 1016
SfaNI GCATC 6 cut(s) 34, 262, 546, 605, 666, 1107
SfcI CTRYAG 2 cut(s) 148, 501
SinI GGWCC 3 cut(s) 1147, 1281, 1531
SmaI CCCGGG 1 cut(s) 765
SmiMI CAYNNNNRTG 2 cut(s) 1206, 1388
SsiI CCGC 1 cut(s) 53
SspMI CTAG 1 cut(s) 1278
StyD4I CCNGG 3 cut(s) 762, 763, 921
TaaI ACNGT 4 cut(s) 14, 833, 874, 1515
TaiI ACGT 1 cut(s) 1206
TaqI TCGA 7 cut(s) 189, 367, 713, 758, 999, 1410, 1464
TaqII GACCGA 1 cut(s) 599
TatI WGTACW 1 cut(s) 941
TauI GCSGC 1 cut(s) 55
TfiI GAWTC 4 cut(s) 65, 176, 725, 1294
Tru1I TTAA 3 cut(s) 21, 318, 1055
Tru9I TTAA 3 cut(s) 21, 318, 1055
TscAI CASTG 5 cut(s) 17, 209, 505, 1390, 1494
TseFI GTSAC 3 cut(s) 550, 1085, 1515
TseI GCWGC 4 cut(s) 161, 806, 1325, 1484
Tsp45I GTSAC 3 cut(s) 550, 1085, 1515
TspGWI ACGGA 1 cut(s) 1541
TspMI CCCGGG 1 cut(s) 763
TspRI CASTG 5 cut(s) 17, 209, 505, 1390, 1494
VpaK11BI GGWCC 3 cut(s) 1147, 1281, 1531
XapI RAATTY 2 cut(s) 127, 447
XmaI CCCGGG 1 cut(s) 763
XmiI GTMKAC 1 cut(s) 1068
XmnI GAANNNNTTC 1 cut(s) 363
XspI CTAG 1 cut(s) 1278
Zsp2I ATGCAT 1 cut(s) 1122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.