Rorug02G0504800

tryptophan synthase beta chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0011129
Physical Location & Seq
Forward (+)
63622835 .. 63626830
3996 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0504800.1

Sequence Viewer

Length: 1329 bp
ATGGCTGCCCACCTGAGTCCTTGTTCGTCTTCTTCAGCCTTATTCACACCCTCTTCACTCAAGAACAAGACTACCCACTTCACCAAGTTCATCACTTTGATAAGAGAAGGTCATTCCTCCAGATGGGTCTCTCAGATTCGCCCCAGAAATCGGTTTCAGCTCAAATCCTCAAATGGGCATCCACTCAATGCCCTGTCTTTTCATGGCGATGGAAATTCTGTGGGCCAAGAACAATTTGAGCCTGGTGGCAAAGAGGGTACCTTTCCTGTCTTAGATTCGAAAAATGTGGGATCTAAGTTGAGCATAACTGTTGTTGGTGCTTCTGGAGACCTTGCCAAGAAGAAGATATTCCCAGCACTTTTTGCTCTTTATTATGAAGATTGTCTACCTGAGGATTTTACAGTCTTTGGTTTTGCTCGAACTAAAATGACTGATGAAGAGCTAAGGAACATGATCAGCAGAACCTTGACTTGCAGAATAGATAAGAGGGCAAATTGTGAGGACAAAATGGACCAGTTCTTGAAAAGATGCTTTTACCATTCTGGTCTGTATAATTCTGAGCAGAGTTTTGCAGAATTGGACATCAAGCTGAAAGAGAAGGAGGCTGGAAGAAAGTCAAATAGGCTGTTTTACTTATCAATACCTCCAAACATATTTGTGGATGTGGTGAGATGTGCTAGTCTTAGAGCTTCTTCAGAAAATGGGTGGACCAGGCTCATTGTTGAGAAGCCATTTGGTCGTGACTCTGAATCATCTGCGGAGCTAACCAGATGTTTAAAGCAGTACCTTGCTGAAGACCAGATATTTAGGATTGACCATTACTTGGGGAAGGAGCTTGTCGAGAATCTATCAGTGCTTCGCTTCTCAAATCTTGTTTTTGAGCCTGTCTGGTCACGTAACTACATTCGCAATGTGCAACTGATCTTTTCTGAAGATTTTGGCACAGAGGGAAGAGGGGGATATTTTGATAAGTACGGAATCATACGGGACATAATGCAAAATCATCTTCTGCAAATACTAGCACTGTTTGCGATGGAAACACCGGTCAGCTTAGATGCTGAGGACATCAGGAATGAAAAGGTAAAGGTTCTAAGATCAATGAGGCCACTGCAGCTTGAAGATGTAGTTGTTGGCCAGTATAAGGGCCACAGCAAGGGCAGTAAATCTTTTCCTGCTTATATAGATGACCCAACTGTGCCAAACGACAGTCTCACCCCTACATTTGCAGCAGCAGCTCTCTTTATTGATAATGCCAGATGGGATGGAGTTCCTTTCCTGATGAAAGCAGGAAAGGCCCTCCATACCAAGCGAGATCAGAGTTCAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00400 ko01100 ko01110 ko01130 ko01230 map00260 map00400 map01100 map01110 map01130 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

442

Amino Acids

50.01

Weight (kDa)

8.78

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G6PD_N PF00479 104 - 282 7.3e-58 Glucose-6-phosphate dehydrogenase, NAD binding domain
G6PD_C PF02781 285 - 438 5.7e-66 Glucose-6-phosphate dehydrogenase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011129)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67170 AT1G67170
fragaria_vesca FvH4_6g45670 FvH4_6g45670 FvH4_6g45670
malus_domestica MD09G1080900.v1.1 MD17G1072000.v1.1
prunus_persica Prupe.3G242800_v2.0.a1
pyrus_communis pycom09g00730 pycom17g07130
rosa_chinensis RchiOBHm_Chr2g0163821
rosa_laevigata RLG00000021418
rosa_multiflora Rmu_sc0001181.1_g000004
rosa_roxburghii Rroxscaffold_2G00086990
rosa_rugosa Rorug02G0504800
rosa_samantha Rh2AG572700 Rh2BG583400 Rh2CG554300 Rh2DG593000
rosa_wichuraiana Rw2G047350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 257
AccB1I GGYRCC 1 cut(s) 257
AccB7I CCANNNNNTGG 1 cut(s) 823
AccI GTMKAC 1 cut(s) 385
AciI CCGC 1 cut(s) 758
AclWI GGATC 1 cut(s) 298
AcoI YGGCCR 1 cut(s) 1132
AcsI RAATTY 1 cut(s) 214
AcuI CTGAAG 4 cut(s) 18, 678, 813, 951
AfaI GTAC 3 cut(s) 259, 785, 974
AfiI CCNNNNNNNGG 6 cut(s) 123, 150, 174, 823, 1141, 1153
AgeI ACCGGT 1 cut(s) 1042
AgsI TTSAA 3 cut(s) 523, 1118, 1323
AjnI CCWGG 2 cut(s) 241, 710
AluBI AGCT 9 cut(s) 160, 442, 589, 689, 763, 835, 1050, 1114, 1235
AluI AGCT 9 cut(s) 160, 442, 589, 689, 763, 835, 1050, 1114, 1235
Alw26I GTCTC 3 cut(s) 133, 321, 1214
AlwI GGATC 1 cut(s) 298
AoxI GGCC 5 cut(s) 223, 1103, 1132, 1144, 1293
ApeKI GCWGC 5 cut(s) 5, 1111, 1226, 1229, 1232
ApoI RAATTY 1 cut(s) 214
AsiGI ACCGGT 1 cut(s) 1042
Asp700I GAANNNNTTC 1 cut(s) 347
Asp718I GGTACC 1 cut(s) 257
AspS9I GGNCC 5 cut(s) 223, 511, 708, 1144, 1294
AsuHPI GGTGA 3 cut(s) 73, 679, 1204
AsuII TTCGAA 1 cut(s) 278
AvaII GGWCC 2 cut(s) 511, 708
AxyI CCTNAGG 1 cut(s) 390
BalI TGGCCA 1 cut(s) 1134
BanI GGYRCC 1 cut(s) 257
BarI GAAGNNNNNNTAC 2 cut(s) 369, 401
BbsI GAAGAC 2 cut(s) 21, 801
BbvCI CCTCAGC 1 cut(s) 1059
BbvI GCAGC 4 cut(s) 1123, 1238, 1241, 1244
BccI CCATC 5 cut(s) 117, 203, 1027, 1251, 1256
BciT130I CCWGG 2 cut(s) 243, 712
BclI TGATCA 1 cut(s) 453
BcoDI GTCTC 3 cut(s) 133, 321, 1214
BfaI CTAG 2 cut(s) 678, 1019
BfmI CTRYAG 1 cut(s) 1109
BisI GCNGC 5 cut(s) 6, 1112, 1227, 1230, 1233
BlsI GCNGC 5 cut(s) 7, 1113, 1228, 1231, 1234
Bme1390I CCNGG 2 cut(s) 243, 712
Bme18I GGWCC 2 cut(s) 511, 708
BmgT120I GGNCC 5 cut(s) 223, 511, 708, 1144, 1294
BmiI GGNNCC 1 cut(s) 259
BmrFI CCNGG 2 cut(s) 243, 712
BmsI GCATC 3 cut(s) 187, 518, 1045
BpiI GAAGAC 2 cut(s) 21, 801
BpmI CTGGAG 2 cut(s) 103, 345
Bpu10I CCTNAGC 2 cut(s) 443, 1059
Bpu14I TTCGAA 1 cut(s) 278
BpuEI CTTGAG 1 cut(s) 44
BsaAI YACGTR 1 cut(s) 896
BsaI GGTCTC 2 cut(s) 133, 321
BsaWI WCCGGW 1 cut(s) 1042
Bsc4I CCNNNNNNNGG 6 cut(s) 123, 150, 174, 823, 1141, 1153
Bse118I RCCGGY 1 cut(s) 1042
Bse1I ACTGG 2 cut(s) 514, 1135
Bse21I CCTNAGG 1 cut(s) 390
Bse3DI GCAATG 1 cut(s) 916
BseBI CCWGG 2 cut(s) 243, 712
BseGI GGATG 3 cut(s) 178, 667, 1267
BseLI CCNNNNNNNGG 6 cut(s) 123, 150, 174, 823, 1141, 1153
BseMI GCAATG 1 cut(s) 916
BseMII CTCAG 5 cut(s) 5, 146, 381, 549, 1050
BseNI ACTGG 2 cut(s) 514, 1135
BseXI GCAGC 4 cut(s) 1123, 1238, 1241, 1244
BseYI CCCAGC 1 cut(s) 352
BshFI GGCC 5 cut(s) 225, 1105, 1134, 1146, 1295
BshNI GGYRCC 1 cut(s) 257
BshTI ACCGGT 1 cut(s) 1042
BsiSI CCGG 1 cut(s) 1043
BslFI GGGAC 1 cut(s) 1001
BslI CCNNNNNNNGG 6 cut(s) 123, 150, 174, 823, 1141, 1153
BsmAI GTCTC 3 cut(s) 133, 321, 1214
BsmFI GGGAC 1 cut(s) 1001
BsnI GGCC 5 cut(s) 225, 1105, 1134, 1146, 1295
Bso31I GGTCTC 2 cut(s) 133, 321
Bsp119I TTCGAA 1 cut(s) 278
Bsp143I GATC 5 cut(s) 290, 453, 921, 1094, 1312
BspACI CCGC 1 cut(s) 758
BspANI GGCC 5 cut(s) 225, 1105, 1134, 1146, 1295
BspCNI CTCAG 5 cut(s) 6, 145, 382, 550, 1051
BspLI GGNNCC 1 cut(s) 259
BspMAI CTGCAG 1 cut(s) 1113
BspPI GGATC 1 cut(s) 298
BspQI GCTCTTC 1 cut(s) 432
BspT104I TTCGAA 1 cut(s) 278
BspT107I GGYRCC 1 cut(s) 257
BspTNI GGTCTC 2 cut(s) 133, 321
BsrDI GCAATG 1 cut(s) 916
BsrFI RCCGGY 1 cut(s) 1042
BsrI ACTGG 2 cut(s) 514, 1135
BssAI RCCGGY 1 cut(s) 1042
BssMI GATC 5 cut(s) 290, 453, 921, 1094, 1312
Bst2UI CCWGG 2 cut(s) 243, 712
Bst4CI ACNGT 5 cut(s) 310, 403, 1026, 1195, 1208
Bst6I CTCTTC 3 cut(s) 58, 432, 946
BstAPI GCANNNNNTGC 2 cut(s) 362, 1028
BstBAI YACGTR 1 cut(s) 896
BstBI TTCGAA 1 cut(s) 278
BstF5I GGATG 3 cut(s) 178, 667, 1267
BstKTI GATC 5 cut(s) 293, 456, 924, 1097, 1315
BstMAI GTCTC 3 cut(s) 133, 321, 1214
BstMBI GATC 5 cut(s) 290, 453, 921, 1094, 1312
BstMWI GCNNNNNNNGC 5 cut(s) 362, 1028, 1111, 1232, 1292
BstNI CCWGG 2 cut(s) 243, 712
BstSCI CCNGG 2 cut(s) 241, 710
BstSFI CTRYAG 1 cut(s) 1109
BstV1I GCAGC 4 cut(s) 1123, 1238, 1241, 1244
BstV2I GAAGAC 2 cut(s) 21, 801
BstX2I RGATCY 1 cut(s) 290
BstYI RGATCY 1 cut(s) 290
Bsu36I CCTNAGG 1 cut(s) 390
BsuRI GGCC 5 cut(s) 225, 1105, 1134, 1146, 1295
BtgZI GCGATG 2 cut(s) 222, 1046
BtsCI GGATG 3 cut(s) 178, 667, 1267
BtsI GCAGTG 1 cut(s) 1106
BtsIMutI CAGTG 3 cut(s) 858, 1022, 1106
Cfr10I RCCGGY 1 cut(s) 1042
Cfr13I GGNCC 5 cut(s) 223, 511, 708, 1144, 1294
Csp6I GTAC 3 cut(s) 258, 784, 973
CspAI ACCGGT 1 cut(s) 1042
CviAII CATG 2 cut(s) 203, 451
CviQI GTAC 3 cut(s) 258, 784, 973
DpnI GATC 5 cut(s) 292, 455, 923, 1096, 1314
DpnII GATC 5 cut(s) 290, 453, 921, 1094, 1312
DraI TTTAAA 1 cut(s) 777
EaeI YGGCCR 1 cut(s) 1132
Eam1104I CTCTTC 3 cut(s) 58, 432, 946
EarI CTCTTC 3 cut(s) 58, 432, 946
Eco31I GGTCTC 2 cut(s) 133, 321
Eco47I GGWCC 2 cut(s) 511, 708
Eco57I CTGAAG 4 cut(s) 18, 678, 813, 951
Eco81I CCTNAGG 1 cut(s) 390
EcoO109I RGGNCCY 1 cut(s) 1294
EcoRII CCWGG 2 cut(s) 241, 710
FaeI CATG 2 cut(s) 206, 454
FaqI GGGAC 1 cut(s) 1001
FatI CATG 2 cut(s) 202, 450
FbaI TGATCA 1 cut(s) 453
FblI GTMKAC 1 cut(s) 385
Fnu4HI GCNGC 5 cut(s) 6, 1112, 1227, 1230, 1233
FokI GGATG 3 cut(s) 165, 674, 1274
Fsp4HI GCNGC 5 cut(s) 6, 1112, 1227, 1230, 1233
FspBI CTAG 2 cut(s) 678, 1019
GluI GCNGC 5 cut(s) 6, 1112, 1227, 1230, 1233
GsaI CCCAGC 1 cut(s) 356
GsuI CTGGAG 2 cut(s) 103, 345
HaeIII GGCC 5 cut(s) 225, 1105, 1134, 1146, 1295
HapII CCGG 1 cut(s) 1043
Hin1II CATG 2 cut(s) 206, 454
HinfI GANTC 7 cut(s) 16, 136, 275, 743, 749, 844, 978
HpaII CCGG 1 cut(s) 1043
HphI GGTGA 3 cut(s) 73, 679, 1204
Hpy166II GTNNAC 2 cut(s) 386, 708
Hpy188I TCNGA 6 cut(s) 135, 559, 697, 748, 931, 1317
Hpy188III TCNNGA 8 cut(s) 61, 120, 324, 520, 740, 841, 1069, 1276
Hpy8I GTNNAC 2 cut(s) 386, 708
HpyAV CCTTC 3 cut(s) 101, 592, 823
HpyCH4III ACNGT 5 cut(s) 310, 403, 1026, 1195, 1208
HpyCH4IV ACGT 1 cut(s) 895
HpyCH4V TGCA 7 cut(s) 474, 572, 916, 997, 1012, 1111, 1226
HpyF10VI GCNNNNNNNGC 5 cut(s) 362, 1028, 1111, 1232, 1292
HpySE526I ACGT 1 cut(s) 895
Hsp92II CATG 2 cut(s) 206, 454
KpnI GGTACC 1 cut(s) 261
Ksp22I TGATCA 1 cut(s) 453
Kzo9I GATC 5 cut(s) 290, 453, 921, 1094, 1312
LguI GCTCTTC 1 cut(s) 432
LmnI GCTCC 2 cut(s) 760, 832
Lsp1109I GCAGC 4 cut(s) 1123, 1238, 1241, 1244
LweI GCATC 3 cut(s) 187, 518, 1045
MaeI CTAG 2 cut(s) 678, 1019
MaeII ACGT 1 cut(s) 895
MaeIII GTNAC 3 cut(s) 740, 891, 896
MalI GATC 5 cut(s) 292, 455, 923, 1096, 1314
MboI GATC 5 cut(s) 290, 453, 921, 1094, 1312
MflI RGATCY 1 cut(s) 290
MlsI TGGCCA 1 cut(s) 1134
MluCI AATT 6 cut(s) 214, 233, 493, 553, 575, 1323
MluNI TGGCCA 1 cut(s) 1134
MlyI GAGTC 2 cut(s) 25, 737
Mox20I TGGCCA 1 cut(s) 1134
MroXI GAANNNNTTC 1 cut(s) 347
MscI TGGCCA 1 cut(s) 1134
MseI TTAA 1 cut(s) 776
MslI CAYNNNNRTG 2 cut(s) 207, 656
Msp20I TGGCCA 1 cut(s) 1134
MspI CCGG 1 cut(s) 1043
MspR9I CCNGG 2 cut(s) 243, 712
MvaI CCWGG 2 cut(s) 243, 712
MwoI GCNNNNNNNGC 5 cut(s) 362, 1028, 1111, 1232, 1292
NdeII GATC 5 cut(s) 290, 453, 921, 1094, 1312
NlaIII CATG 2 cut(s) 206, 454
NlaIV GGNNCC 1 cut(s) 259
NmuCI GTSAC 2 cut(s) 740, 891
NspV TTCGAA 1 cut(s) 278
PciSI GCTCTTC 1 cut(s) 432
PdmI GAANNNNTTC 1 cut(s) 347
PfeI GAWTC 5 cut(s) 136, 275, 749, 844, 978
PflMI CCANNNNNTGG 1 cut(s) 823
PinAI ACCGGT 1 cut(s) 1042
PkrI GCNGC 5 cut(s) 7, 1113, 1228, 1231, 1234
PleI GAGTC 2 cut(s) 24, 737
PpsI GAGTC 2 cut(s) 24, 737
Ppu21I YACGTR 1 cut(s) 896
Psp6I CCWGG 2 cut(s) 241, 710
PspFI CCCAGC 1 cut(s) 352
PspGI CCWGG 2 cut(s) 241, 710
PspN4I GGNNCC 1 cut(s) 259
PspPI GGNCC 5 cut(s) 223, 511, 708, 1144, 1294
PstI CTGCAG 1 cut(s) 1113
PsuI RGATCY 1 cut(s) 290
RsaI GTAC 3 cut(s) 259, 785, 974
RsaNI GTAC 3 cut(s) 258, 784, 973
RseI CAYNNNNRTG 2 cut(s) 207, 656
SapI GCTCTTC 1 cut(s) 432
SaqAI TTAA 1 cut(s) 776
SatI GCNGC 5 cut(s) 6, 1112, 1227, 1230, 1233
Sau3AI GATC 5 cut(s) 290, 453, 921, 1094, 1312
Sau96I GGNCC 5 cut(s) 223, 511, 708, 1144, 1294
SchI GAGTC 2 cut(s) 25, 737
ScrFI CCNGG 2 cut(s) 243, 712
SfaNI GCATC 3 cut(s) 187, 518, 1045
SfcI CTRYAG 1 cut(s) 1109
SfuI TTCGAA 1 cut(s) 278
SinI GGWCC 2 cut(s) 511, 708
SmiMI CAYNNNNRTG 2 cut(s) 207, 656
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 6 cut(s) 214, 233, 493, 553, 575, 1323
SsiI CCGC 1 cut(s) 758
SspMI CTAG 2 cut(s) 678, 1019
StyD4I CCNGG 2 cut(s) 241, 710
TaaI ACNGT 5 cut(s) 310, 403, 1026, 1195, 1208
TaiI ACGT 1 cut(s) 898
TaqI TCGA 3 cut(s) 278, 418, 840
TasI AATT 6 cut(s) 214, 233, 493, 553, 575, 1323
TfiI GAWTC 5 cut(s) 136, 275, 749, 844, 978
Tru1I TTAA 1 cut(s) 776
Tru9I TTAA 1 cut(s) 776
TscAI CASTG 3 cut(s) 858, 1029, 1113
TseFI GTSAC 2 cut(s) 740, 891
TseI GCWGC 5 cut(s) 5, 1111, 1226, 1229, 1232
Tsp45I GTSAC 2 cut(s) 740, 891
TspDTI ATGAA 6 cut(s) 79, 191, 390, 450, 1089, 1295
TspGWI ACGGA 1 cut(s) 990
TspRI CASTG 3 cut(s) 858, 1029, 1113
Van91I CCANNNNNTGG 1 cut(s) 823
VpaK11BI GGWCC 2 cut(s) 511, 708
XapI RAATTY 1 cut(s) 214
XmiI GTMKAC 1 cut(s) 385
XmnI GAANNNNTTC 1 cut(s) 347
XspI CTAG 2 cut(s) 678, 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.