pycom17g07130

FLX-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0011129
Physical Location & Seq
Forward (+)
5137136 .. 5138073
938 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g07130.2

Sequence Viewer

Length: 723 bp
ATGGGAAGCAAAGGTCGAATTCCACCGGCTCACATGCGTCGGCCGCTCTCCGGACCGGACCCATTTGGCCCGGGGATGCGGCCGCCCCATGGCGCCTATGCTCCGTTCGATATGTTGCCTCCTCCGCAAGTTATGGAGCAGAAGCTGGCTGCACAACATGTGGAGATGCAGAGGCTTGTGGTGGAGAACCAGAGGCTCGCCGCCACCCACGGAAGCCTGAGGCAAGAGCTGGCGGGTGCCCAACATGAGTTGCAGATATTACATGCCCAGATAGGAGCTATCAAGTCTGAGAGAGAACAGCAGATGAGTAGCCTTGTGGATAGTATAGCTAAGATGGAAGCTGACCTGAAGTCGGCTGAGCCTGTTAAGGCGGAGCTACAGAAGGCTCGAGCCGAGGCCCAGAGCTTGGTTGTTTCGAGGCAAGAACTCATCACCAAAGTGCAACAACTTGGGCAGGATCTTCAGAGGACTCATGCAGATGTGCAGCAGATTCCGGCTTTGGTTGCACAGCTTGATGGACTGAGGCAGGAATATCAGCTATGCAGGGATACATATGACTATGAAAAGAGATTATACAGCAGCCACCTTGAATCACTTCAGGTCATGGAGAAGAACTATGTTACCATGGCTAGGGAGGTGGAAAAGCTCCGAGCAGAGTTACTGAATAATTCTAATGTTCGATCGTCGAACTGGTGCCCCATATTATGGAACCCCGGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

241

Amino Acids

27.05

Weight (kDa)

7.83

Isoelectric Point (pI)

52.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011129)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67170 AT1G67170
fragaria_vesca FvH4_6g45670 FvH4_6g45670 FvH4_6g45670
malus_domestica MD09G1080900.v1.1 MD17G1072000.v1.1
prunus_persica Prupe.3G242800_v2.0.a1
pyrus_communis pycom09g00730 pycom17g07130
rosa_chinensis RchiOBHm_Chr2g0163821
rosa_laevigata RLG00000021418
rosa_multiflora Rmu_sc0001181.1_g000004
rosa_roxburghii Rroxscaffold_2G00086990
rosa_rugosa Rorug02G0504800
rosa_samantha Rh2AG572700 Rh2BG583400 Rh2CG554300 Rh2DG593000
rosa_wichuraiana Rw2G047350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 92, 236, 693
AccB7I CCANNNNNTGG 2 cut(s) 406, 705
AccBSI CCGCTC 1 cut(s) 46
AccIII TCCGGA 1 cut(s) 50
AciI CCGC 7 cut(s) 44, 79, 83, 125, 201, 233, 371
AclWI GGATC 1 cut(s) 465
AcoI YGGCCR 2 cut(s) 41, 80
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 3 cut(s) 368, 446, 581
AcyI GRCGYC 1 cut(s) 93
AfiI CCNNNNNNNGG 6 cut(s) 50, 89, 352, 406, 630, 705
AflIII ACRYGT 1 cut(s) 157
AgsI TTSAA 1 cut(s) 590
AhdI GACNNNNNGTC 1 cut(s) 349
AleI CACNNNNGTG 1 cut(s) 437
AlwI GGATC 1 cut(s) 465
AlwNI CAGNNNCTG 1 cut(s) 145
Ama87I CYCGRG 3 cut(s) 70, 387, 713
Aor13HI TCCGGA 1 cut(s) 50
AoxI GGCC 4 cut(s) 41, 67, 80, 396
ApeKI GCWGC 3 cut(s) 149, 484, 579
ApoI RAATTY 1 cut(s) 18
Asp700I GAANNNNTTC 1 cut(s) 594
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 4 cut(s) 53, 58, 68, 397
AsuC2I CCSGG 4 cut(s) 71, 72, 714, 715
AsuHPI GGTGA 1 cut(s) 424
AvaI CYCGRG 3 cut(s) 70, 387, 713
AvaII GGWCC 2 cut(s) 53, 58
AxyI CCTNAGG 1 cut(s) 218
BaeGI GKGCMC 2 cut(s) 241, 698
BanI GGYRCC 3 cut(s) 92, 236, 693
BbvI GCAGC 3 cut(s) 136, 496, 591
BccI CCATC 2 cut(s) 328, 509
BciVI GTATCC 1 cut(s) 541
BcnI CCSGG 4 cut(s) 71, 72, 714, 715
BfaI CTAG 1 cut(s) 630
BfmI CTRYAG 1 cut(s) 377
BfoI RGCGCY 1 cut(s) 96
BfuI GTATCC 1 cut(s) 541
BisI GCNGC 7 cut(s) 44, 80, 83, 150, 201, 485, 580
BlpI GCTNAGC 1 cut(s) 357
BlsI GCNGC 7 cut(s) 45, 81, 84, 151, 202, 486, 581
Bme1390I CCNGG 4 cut(s) 71, 72, 714, 715
Bme18I GGWCC 2 cut(s) 53, 58
BmeRI GACNNNNNGTC 1 cut(s) 349
BmeT110I CYCGRG 3 cut(s) 70, 387, 713
BmgT120I GGNCC 4 cut(s) 53, 58, 68, 397
BmiI GGNNCC 5 cut(s) 60, 94, 238, 695, 710
BmrFI CCNGG 4 cut(s) 71, 72, 714, 715
BmsI GCATC 2 cut(s) 66, 156
Bpu1102I GCTNAGC 1 cut(s) 357
BpuMI CCSGG 4 cut(s) 71, 72, 714, 715
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 8 cut(s) 70, 71, 88, 208, 393, 624, 712, 713
BsaWI WCCGGW 2 cut(s) 50, 55
Bsc4I CCNNNNNNNGG 6 cut(s) 50, 89, 352, 406, 630, 705
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 695
Bse21I CCTNAGG 1 cut(s) 218
BseAI TCCGGA 1 cut(s) 50
BseDI CCNNGG 8 cut(s) 70, 71, 88, 208, 393, 624, 712, 713
BseGI GGATG 1 cut(s) 81
BseLI CCNNNNNNNGG 6 cut(s) 50, 89, 352, 406, 630, 705
BseMII CTCAG 4 cut(s) 209, 279, 348, 512
BseNI ACTGG 1 cut(s) 695
BseRI GAGGAG 1 cut(s) 111
BseSI GKGCMC 2 cut(s) 241, 698
BseX3I CGGCCG 2 cut(s) 41, 80
BseXI GCAGC 3 cut(s) 136, 496, 591
BsgI GTGCAG 2 cut(s) 135, 503
Bsh1285I CGRYCG 3 cut(s) 44, 83, 683
BshFI GGCC 4 cut(s) 43, 69, 82, 398
BshNI GGYRCC 3 cut(s) 92, 236, 693
BsiEI CGRYCG 3 cut(s) 44, 83, 683
BsiHKCI CYCGRG 3 cut(s) 70, 387, 713
BsiSI CCGG 6 cut(s) 26, 51, 56, 71, 494, 714
BslI CCNNNNNNNGG 6 cut(s) 50, 89, 352, 406, 630, 705
BsnI GGCC 4 cut(s) 43, 69, 82, 398
BsoBI CYCGRG 3 cut(s) 70, 387, 713
Bsp1286I GDGCHC 2 cut(s) 241, 698
Bsp13I TCCGGA 1 cut(s) 50
Bsp143I GATC 2 cut(s) 457, 680
Bsp1720I GCTNAGC 1 cut(s) 357
Bsp19I CCATGG 2 cut(s) 88, 624
BspACI CCGC 7 cut(s) 44, 79, 83, 125, 201, 233, 371
BspANI GGCC 4 cut(s) 43, 69, 82, 398
BspCNI CTCAG 4 cut(s) 210, 280, 349, 513
BspEI TCCGGA 1 cut(s) 50
BspLI GGNNCC 5 cut(s) 60, 94, 238, 695, 710
BspPI GGATC 1 cut(s) 465
BspT107I GGYRCC 3 cut(s) 92, 236, 693
BsrBI CCGCTC 1 cut(s) 46
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 1 cut(s) 695
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 8 cut(s) 70, 71, 88, 208, 393, 624, 712, 713
BssMI GATC 2 cut(s) 457, 680
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 2 cut(s) 88, 624
BstACI GRCGYC 1 cut(s) 93
BstC8I GCNNGC 3 cut(s) 147, 198, 231
BstDEI CTNAG 5 cut(s) 218, 288, 330, 357, 521
BstDSI CCRYGG 3 cut(s) 88, 208, 624
BstF5I GGATG 1 cut(s) 81
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 2 cut(s) 460, 683
BstMBI GATC 2 cut(s) 457, 680
BstMCI CGRYCG 3 cut(s) 44, 83, 683
BstMWI GCNNNNNNNGC 3 cut(s) 43, 124, 503
BstNSI RCATGY 3 cut(s) 37, 161, 266
BstSCI CCNGG 4 cut(s) 69, 70, 712, 713
BstSFI CTRYAG 1 cut(s) 377
BstSLI GKGCMC 2 cut(s) 241, 698
BstV1I GCAGC 3 cut(s) 136, 496, 591
BstX2I RGATCY 1 cut(s) 457
BstYI RGATCY 1 cut(s) 457
BstZI CGGCCG 2 cut(s) 41, 80
Bsu36I CCTNAGG 1 cut(s) 218
BsuI GTATCC 1 cut(s) 541
BsuRI GGCC 4 cut(s) 43, 69, 82, 398
BtgI CCRYGG 3 cut(s) 88, 208, 624
BtsCI GGATG 1 cut(s) 81
Cac8I GCNNGC 3 cut(s) 147, 198, 231
CaiI CAGNNNCTG 1 cut(s) 145
CciNI GCGGCCGC 1 cut(s) 80
CfoI GCGC 1 cut(s) 95
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 4 cut(s) 53, 58, 68, 397
Cfr9I CCCGGG 2 cut(s) 70, 713
CpoI CGGWCCG 1 cut(s) 53
CseI GACGC 1 cut(s) 26
CspI CGGWCCG 1 cut(s) 53
CviAII CATG 8 cut(s) 34, 89, 158, 245, 263, 473, 604, 625
DdeI CTNAG 5 cut(s) 218, 288, 330, 357, 521
DinI GGCGCC 1 cut(s) 94
DpnI GATC 2 cut(s) 459, 682
DpnII GATC 2 cut(s) 457, 680
DriI GACNNNNNGTC 1 cut(s) 349
EaeI YGGCCR 2 cut(s) 41, 80
EagI CGGCCG 2 cut(s) 41, 80
Eam1105I GACNNNNNGTC 1 cut(s) 349
EciI GGCGGA 1 cut(s) 386
EclXI CGGCCG 2 cut(s) 41, 80
Eco130I CCWWGG 2 cut(s) 88, 624
Eco47I GGWCC 2 cut(s) 53, 58
Eco52I CGGCCG 2 cut(s) 41, 80
Eco57I CTGAAG 3 cut(s) 368, 446, 581
Eco81I CCTNAGG 1 cut(s) 218
Eco88I CYCGRG 3 cut(s) 70, 387, 713
EcoRI GAATTC 1 cut(s) 18
EcoT14I CCWWGG 2 cut(s) 88, 624
EgeI GGCGCC 1 cut(s) 94
EheI GGCGCC 1 cut(s) 94
ErhI CCWWGG 2 cut(s) 88, 624
FaeI CATG 8 cut(s) 37, 92, 161, 248, 266, 476, 607, 628
FatI CATG 8 cut(s) 33, 88, 157, 244, 262, 472, 603, 624
FauI CCCGC 1 cut(s) 226
FauNDI CATATG 1 cut(s) 553
Fnu4HI GCNGC 7 cut(s) 44, 80, 83, 150, 201, 485, 580
FokI GGATG 1 cut(s) 88
Fsp4HI GCNGC 7 cut(s) 44, 80, 83, 150, 201, 485, 580
FspBI CTAG 1 cut(s) 630
GlaI GCGC 1 cut(s) 94
GluI GCNGC 7 cut(s) 44, 80, 83, 150, 201, 485, 580
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 4 cut(s) 43, 69, 82, 398
HapII CCGG 6 cut(s) 26, 51, 56, 71, 494, 714
HgaI GACGC 1 cut(s) 26
HhaI GCGC 1 cut(s) 95
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 8 cut(s) 37, 92, 161, 248, 266, 476, 607, 628
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HinfI GANTC 3 cut(s) 469, 490, 590
HpaII CCGG 6 cut(s) 26, 51, 56, 71, 494, 714
HphI GGTGA 1 cut(s) 424
Hpy188I TCNGA 3 cut(s) 289, 465, 650
Hpy188III TCNNGA 1 cut(s) 51
Hpy99I CGWCG 2 cut(s) 42, 688
HpyAV CCTTC 1 cut(s) 376
HpyCH4V TGCA 8 cut(s) 152, 169, 253, 442, 476, 484, 506, 543
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 124, 503
HpyF3I CTNAG 5 cut(s) 218, 288, 330, 357, 521
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 8 cut(s) 37, 92, 161, 248, 266, 476, 607, 628
HspAI GCGC 1 cut(s) 93
KasI GGCGCC 1 cut(s) 92
Kpn2I TCCGGA 1 cut(s) 50
Kzo9I GATC 2 cut(s) 457, 680
LmnI GCTCC 5 cut(s) 106, 136, 275, 373, 651
Lsp1109I GCAGC 3 cut(s) 136, 496, 591
LweI GCATC 2 cut(s) 66, 156
MaeI CTAG 1 cut(s) 630
MaeIII GTNAC 2 cut(s) 619, 657
MalI GATC 2 cut(s) 459, 682
MbiI CCGCTC 1 cut(s) 46
MboI GATC 2 cut(s) 457, 680
MboII GAAGA 2 cut(s) 452, 622
MflI RGATCY 1 cut(s) 457
MhlI GDGCHC 2 cut(s) 241, 698
MluCI AATT 2 cut(s) 18, 667
Mly113I GGCGCC 1 cut(s) 93
MlyI GAGTC 1 cut(s) 463
MroI TCCGGA 1 cut(s) 50
MroXI GAANNNNTTC 1 cut(s) 594
MseI TTAA 1 cut(s) 366
MslI CAYNNNNRTG 2 cut(s) 437, 477
MspI CCGG 6 cut(s) 26, 51, 56, 71, 494, 714
MspR9I CCNGG 4 cut(s) 71, 72, 714, 715
MwoI GCNNNNNNNGC 3 cut(s) 43, 124, 503
NarI GGCGCC 1 cut(s) 93
NciI CCSGG 4 cut(s) 71, 72, 714, 715
NcoI CCATGG 2 cut(s) 88, 624
NdeI CATATG 1 cut(s) 553
NdeII GATC 2 cut(s) 457, 680
NlaIII CATG 8 cut(s) 37, 92, 161, 248, 266, 476, 607, 628
NlaIV GGNNCC 5 cut(s) 60, 94, 238, 695, 710
NmeAIII GCCGAG 1 cut(s) 418
NotI GCGGCCGC 1 cut(s) 80
NspI RCATGY 3 cut(s) 37, 161, 266
OliI CACNNNNGTG 1 cut(s) 437
PaeR7I CTCGAG 1 cut(s) 387
PciI ACATGT 1 cut(s) 157
PdmI GAANNNNTTC 1 cut(s) 594
PfeI GAWTC 2 cut(s) 490, 590
PflMI CCANNNNNTGG 2 cut(s) 406, 705
PkrI GCNGC 7 cut(s) 45, 81, 84, 151, 202, 486, 581
Ple19I CGATCG 1 cut(s) 683
PleI GAGTC 1 cut(s) 463
PluTI GGCGCC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 463
PscI ACATGT 1 cut(s) 157
PspN4I GGNNCC 5 cut(s) 60, 94, 238, 695, 710
PspPI GGNCC 4 cut(s) 53, 58, 68, 397
PspXI VCTCGAGB 1 cut(s) 387
PstNI CAGNNNCTG 1 cut(s) 145
PsuI RGATCY 1 cut(s) 457
PvuI CGATCG 1 cut(s) 683
RseI CAYNNNNRTG 2 cut(s) 437, 477
Rsr2I CGGWCCG 1 cut(s) 53
RsrII CGGWCCG 1 cut(s) 53
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 7 cut(s) 44, 80, 83, 150, 201, 485, 580
Sau3AI GATC 2 cut(s) 457, 680
Sau96I GGNCC 4 cut(s) 53, 58, 68, 397
SchI GAGTC 1 cut(s) 463
ScrFI CCNGG 4 cut(s) 71, 72, 714, 715
SduI GDGCHC 2 cut(s) 241, 698
SfaNI GCATC 2 cut(s) 66, 156
SfcI CTRYAG 1 cut(s) 377
SfoI GGCGCC 1 cut(s) 94
Sfr274I CTCGAG 1 cut(s) 387
SinI GGWCC 2 cut(s) 53, 58
SlaI CTCGAG 1 cut(s) 387
SmaI CCCGGG 2 cut(s) 72, 715
SmiMI CAYNNNNRTG 2 cut(s) 437, 477
SmlI CTYRAG 1 cut(s) 387
SmoI CTYRAG 1 cut(s) 387
Sse9I AATT 2 cut(s) 18, 667
SsiI CCGC 7 cut(s) 44, 79, 83, 125, 201, 233, 371
SspDI GGCGCC 1 cut(s) 92
SspMI CTAG 1 cut(s) 630
StyD4I CCNGG 4 cut(s) 69, 70, 712, 713
StyI CCWWGG 2 cut(s) 88, 624
TaqI TCGA 6 cut(s) 16, 108, 388, 416, 679, 686
TasI AATT 2 cut(s) 18, 667
TauI GCSGC 4 cut(s) 46, 82, 85, 203
TfiI GAWTC 2 cut(s) 490, 590
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TseI GCWGC 3 cut(s) 149, 484, 579
TspDTI ATGAA 1 cut(s) 576
TspGWI ACGGA 2 cut(s) 93, 225
TspMI CCCGGG 2 cut(s) 70, 713
Van91I CCANNNNNTGG 2 cut(s) 406, 705
VpaK11BI GGWCC 2 cut(s) 53, 58
XapI RAATTY 1 cut(s) 18
XceI RCATGY 3 cut(s) 37, 161, 266
XhoI CTCGAG 1 cut(s) 387
XmaI CCCGGG 2 cut(s) 70, 713
XmnI GAANNNNTTC 1 cut(s) 594
XspI CTAG 1 cut(s) 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.