FvH4_6g45670

FLX-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011129
Physical Location & Seq
Reverse (-)
35004220 .. 35007368
3149 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g45670.t1

Sequence Viewer

Length: 1218 bp
ATGGGAAGTAAAGGTCGAATCCCACCGGCCCATCTCCGGCGTCCTCATCCGGGACCCGACCCGTATGGGCCTCCTGGGCCGGGTACCCGCCCACCTCCGGGTGGTTACTCACCCTTTGACATGTTGCCTCCTCCGCAAGTTTTGGAGCAGAAGCTGGCTTCACAGCATGTGGAGATGCAGAGGCTGGCAGCTGAGAATCAGAGACTCGCTTCGACGCACGGCAGCCTGAGGCAGGAGCTGGCTGGGGCTCAGCATGAGCTGCAGATACTGCATGCTCAGGCCGGGGCTATGAAGTCAGAGAGGGATCAGCAGATGAGGAGTTTGATGGATAAGATTGCGAAAATGGAGGCTGAGCTGAAATTGGCAGAGCCTGTTAAGGGGGAGCTGCAGCAGGCACGTGCCGAGGCGAATGGCTTGGTTGGGGCGAGGCAGGAGAGTATTGTGAAAGTGCAGCAGTTGAGTCAGGAGCTTCAGAGGGCGCATGTGGAGGTGCAGCAGATTCCGGTTTTGGTTCAGCAACTTGAGGGCCTCAGGCAGGAGTATCAGCATTGCAGGGCTACTTTTGACTATGAAAAGAAATTGTACAATGACCACCTCGAATCACTTCAGGTCATGGAGAAGAACTATGTTACCATGGCTAGGGAGGTAGGAAAACTACGAGCAGAGTTATTGAATAATCCTGGTGTTGAAAGAAGAACTGGACAAAATGCTTATGAAGATAGCTATGGTGTTCCCCAGGGACGTGTCCCATTTCCTCCTGCTGCTGCTGCTGCTGCTGCTGGTGGCAGTGGGCCTGGGGGCAGTGCTGGTGCACCTACTGGTGGCGCTGCTGCTGCAGCCACTGCCGGAACTTCTCCATTTGCCGGAACTCAGCCTGGACATCTGGGAAGGGCCGGTTATGATGCAGCAAGAGGACCTGTTGGTTATGATGCCTCTGGAGGACATGTTGGTTATGATGCACAAAGACCTGGCTATGATCCGCAAAGAGGACCCGTCTATGATGCACAAAGACCAGGTTATGAGGTGCAAAGAGTAGCTGGTTATGATCCAACCCTTGGTCTTAACTATGATGCACAGTCCAGAGGTGCTTCTGGTCCTCAAGGACATATGCCTGCAACCAATATGCCCTATAATTCAGCCGCTCCGCCTACTTCTCGAGCTGGAACTGGATATGAGGTACCACCACCTCGAGGAGGTGCCAACCCAGTTCGTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

406

Amino Acids

43.13

Weight (kDa)

8.77

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011129)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67170 AT1G67170
fragaria_vesca FvH4_6g45670 FvH4_6g45670 FvH4_6g45670
malus_domestica MD09G1080900.v1.1 MD17G1072000.v1.1
prunus_persica Prupe.3G242800_v2.0.a1
pyrus_communis pycom09g00730 pycom17g07130
rosa_chinensis RchiOBHm_Chr2g0163821
rosa_laevigata RLG00000021418
rosa_multiflora Rmu_sc0001181.1_g000004
rosa_roxburghii Rroxscaffold_2G00086990
rosa_rugosa Rorug02G0504800
rosa_samantha Rh2AG572700 Rh2BG583400 Rh2CG554300 Rh2DG593000
rosa_wichuraiana Rw2G047350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1188
Acc65I GGTACC 2 cut(s) 83, 1177
AccB1I GGYRCC 3 cut(s) 83, 1177, 1196
AccB7I CCANNNNNTGG 1 cut(s) 1055
AccBSI CCGCTC 1 cut(s) 1142
AciI CCGC 5 cut(s) 88, 134, 980, 1140, 1145
AclWI GGATC 3 cut(s) 312, 971, 1040
AcuI CTGAAG 2 cut(s) 455, 590
AcvI CACGTG 1 cut(s) 398
AcyI GRCGYC 1 cut(s) 40
AfaI GTAC 3 cut(s) 85, 584, 1179
AflIII ACRYGT 3 cut(s) 120, 742, 943
AgsI TTSAA 2 cut(s) 673, 689
AjiI CACGTC 1 cut(s) 743
AjnI CCWGG 7 cut(s) 73, 679, 735, 793, 874, 967, 1012
Alw21I GWGCWC 1 cut(s) 814
Alw26I GTCTC 1 cut(s) 196
Alw44I GTGCAC 1 cut(s) 810
AlwI GGATC 3 cut(s) 312, 971, 1040
AlwNI CAGNNNCTG 6 cut(s) 154, 184, 238, 268, 371, 842
Ama87I CYCGRG 2 cut(s) 1155, 1188
AoxI GGCC 7 cut(s) 27, 68, 77, 279, 526, 791, 891
ApaLI GTGCAC 1 cut(s) 810
Asp700I GAANNNNTTC 1 cut(s) 603
Asp718I GGTACC 2 cut(s) 83, 1177
AspLEI GCGC 2 cut(s) 481, 827
AsuC2I CCSGG 4 cut(s) 51, 81, 99, 283
AsuHPI GGTGA 1 cut(s) 102
AvaI CYCGRG 2 cut(s) 1155, 1188
AvaII GGWCC 4 cut(s) 53, 914, 989, 1094
AxyI CCTNAGG 2 cut(s) 227, 530
BaeGI GKGCMC 1 cut(s) 814
BanI GGYRCC 3 cut(s) 83, 1177, 1196
BanII GRGCYC 1 cut(s) 250
BbrPI CACGTG 1 cut(s) 398
Bbv12I GWGCWC 1 cut(s) 814
BccI CCATC 2 cut(s) 39, 319
BceAI ACGGC 1 cut(s) 235
BciT130I CCWGG 7 cut(s) 75, 681, 737, 795, 876, 969, 1014
BcnI CCSGG 4 cut(s) 51, 81, 99, 283
BcoDI GTCTC 1 cut(s) 196
BfaI CTAG 1 cut(s) 639
BfmI CTRYAG 3 cut(s) 260, 386, 834
BfoI RGCGCY 1 cut(s) 828
BglI GCCNNNNNGGC 1 cut(s) 76
BlpI GCTNAGC 2 cut(s) 249, 351
Bme18I GGWCC 4 cut(s) 53, 914, 989, 1094
BmeT110I CYCGRG 2 cut(s) 1155, 1188
BmgBI CACGTC 1 cut(s) 743
BmiI GGNNCC 6 cut(s) 54, 55, 85, 991, 1179, 1198
BmrI ACTGGG 1 cut(s) 1199
BmsI GCATC 6 cut(s) 165, 892, 919, 946, 991, 1060
BmuI ACTGGG 1 cut(s) 1199
BpmI CTGGAG 1 cut(s) 957
Bpu10I CCTNAGC 1 cut(s) 276
Bpu1102I GCTNAGC 2 cut(s) 249, 351
BpuEI CTTGAG 2 cut(s) 542, 1083
BpuMI CCSGG 4 cut(s) 51, 81, 99, 283
BsaAI YACGTR 1 cut(s) 398
BsaHI GRCGYC 1 cut(s) 40
BsaJI CCNNGG 8 cut(s) 74, 282, 402, 633, 735, 736, 794, 1054
BsaWI WCCGGW 1 cut(s) 502
BsaXI ACNNNNNCTCC 2 cut(s) 930, 960
Bse118I RCCGGY 2 cut(s) 25, 893
Bse1I ACTGG 4 cut(s) 703, 823, 1171, 1205
Bse21I CCTNAGG 2 cut(s) 227, 530
Bse3DI GCAATG 1 cut(s) 547
BseBI CCWGG 7 cut(s) 75, 681, 737, 795, 876, 969, 1014
BseDI CCNNGG 8 cut(s) 74, 282, 402, 633, 735, 736, 794, 1054
BseGI GGATG 1 cut(s) 46
BseMI GCAATG 1 cut(s) 547
BseMII CTCAG 7 cut(s) 183, 218, 263, 290, 342, 544, 884
BseNI ACTGG 4 cut(s) 703, 823, 1171, 1205
BseRI GAGGAG 3 cut(s) 120, 331, 1206
BseSI GKGCMC 1 cut(s) 814
BseYI CCCAGC 1 cut(s) 242
BsgI GTGCAG 2 cut(s) 470, 512
BshFI GGCC 7 cut(s) 29, 70, 79, 281, 528, 793, 893
BshNI GGYRCC 3 cut(s) 83, 1177, 1196
BsiHKAI GWGCWC 1 cut(s) 814
BsiHKCI CYCGRG 2 cut(s) 1155, 1188
BslFI GGGAC 3 cut(s) 66, 731, 753
BsmAI GTCTC 1 cut(s) 196
BsmFI GGGAC 3 cut(s) 66, 731, 753
BsnI GGCC 7 cut(s) 29, 70, 79, 281, 528, 793, 893
BsoBI CYCGRG 2 cut(s) 1155, 1188
Bsp1286I GDGCHC 2 cut(s) 250, 814
Bsp1407I TGTACA 1 cut(s) 582
Bsp143I GATC 3 cut(s) 304, 976, 1045
Bsp1720I GCTNAGC 2 cut(s) 249, 351
Bsp19I CCATGG 1 cut(s) 633
BspACI CCGC 5 cut(s) 88, 134, 980, 1140, 1145
BspANI GGCC 7 cut(s) 29, 70, 79, 281, 528, 793, 893
BspCNI CTCAG 7 cut(s) 184, 219, 262, 289, 343, 543, 883
BspLI GGNNCC 6 cut(s) 54, 55, 85, 991, 1179, 1198
BspMAI CTGCAG 3 cut(s) 264, 390, 838
BspPI GGATC 3 cut(s) 312, 971, 1040
BspT107I GGYRCC 3 cut(s) 83, 1177, 1196
BsrBI CCGCTC 1 cut(s) 1142
BsrDI GCAATG 1 cut(s) 547
BsrFI RCCGGY 2 cut(s) 25, 893
BsrGI TGTACA 1 cut(s) 582
BsrI ACTGG 4 cut(s) 703, 823, 1171, 1205
BssAI RCCGGY 2 cut(s) 25, 893
BssECI CCNNGG 8 cut(s) 74, 282, 402, 633, 735, 736, 794, 1054
BssMI GATC 3 cut(s) 304, 976, 1045
BssNI GRCGYC 1 cut(s) 40
BssT1I CCWWGG 2 cut(s) 633, 1054
Bst2UI CCWGG 7 cut(s) 75, 681, 737, 795, 876, 969, 1014
Bst4CI ACNGT 1 cut(s) 1077
BstACI GRCGYC 1 cut(s) 40
BstAPI GCANNNNNTGC 3 cut(s) 259, 268, 842
BstAUI TGTACA 1 cut(s) 582
BstBAI YACGTR 1 cut(s) 398
BstC8I GCNNGC 6 cut(s) 156, 186, 240, 273, 393, 1113
BstDEI CTNAG 7 cut(s) 192, 227, 249, 276, 351, 530, 870
BstDSI CCRYGG 1 cut(s) 633
BstENI CCTNNNNNAGG 3 cut(s) 230, 533, 1191
BstF5I GGATG 1 cut(s) 46
BstH2I RGCGCY 1 cut(s) 828
BstHHI GCGC 2 cut(s) 481, 827
BstKTI GATC 3 cut(s) 307, 979, 1048
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 3 cut(s) 304, 976, 1045
BstNI CCWGG 7 cut(s) 75, 681, 737, 795, 876, 969, 1014
BstNSI RCATGY 5 cut(s) 124, 170, 275, 485, 947
BstSFI CTRYAG 3 cut(s) 260, 386, 834
BstSLI GKGCMC 1 cut(s) 814
Bsu36I CCTNAGG 2 cut(s) 227, 530
BsuRI GGCC 7 cut(s) 29, 70, 79, 281, 528, 793, 893
BtgI CCRYGG 1 cut(s) 633
BtrI CACGTC 1 cut(s) 743
BtsCI GGATG 1 cut(s) 46
BtsI GCAGTG 3 cut(s) 793, 808, 840
BtsIMutI CAGTG 3 cut(s) 793, 808, 840
Cac8I GCNNGC 6 cut(s) 156, 186, 240, 273, 393, 1113
CaiI CAGNNNCTG 6 cut(s) 154, 184, 238, 268, 371, 842
CfoI GCGC 2 cut(s) 481, 827
Cfr10I RCCGGY 2 cut(s) 25, 893
CseI GACGC 2 cut(s) 29, 223
CsiI ACCWGGT 1 cut(s) 1012
Csp6I GTAC 3 cut(s) 84, 583, 1178
CviAII CATG 8 cut(s) 121, 167, 254, 272, 482, 613, 634, 944
CviQI GTAC 3 cut(s) 84, 583, 1178
DdeI CTNAG 7 cut(s) 192, 227, 249, 276, 351, 530, 870
DpnI GATC 3 cut(s) 306, 978, 1047
DpnII GATC 3 cut(s) 304, 976, 1045
EciI GGCGGA 1 cut(s) 1134
Eco130I CCWWGG 2 cut(s) 633, 1054
Eco24I GRGCYC 1 cut(s) 250
Eco47I GGWCC 4 cut(s) 53, 914, 989, 1094
Eco57I CTGAAG 2 cut(s) 455, 590
Eco72I CACGTG 1 cut(s) 398
Eco81I CCTNAGG 2 cut(s) 227, 530
Eco88I CYCGRG 2 cut(s) 1155, 1188
EcoNI CCTNNNNNAGG 3 cut(s) 230, 533, 1191
EcoO109I RGGNCCY 4 cut(s) 53, 526, 914, 989
EcoRII CCWGG 7 cut(s) 73, 679, 735, 793, 874, 967, 1012
EcoT14I CCWWGG 2 cut(s) 633, 1054
EcoT38I GRGCYC 1 cut(s) 250
ErhI CCWWGG 2 cut(s) 633, 1054
FaeI CATG 8 cut(s) 124, 170, 257, 275, 485, 616, 637, 947
FaqI GGGAC 3 cut(s) 66, 731, 753
FatI CATG 8 cut(s) 120, 166, 253, 271, 481, 612, 633, 943
FauI CCCGC 1 cut(s) 95
FauNDI CATATG 1 cut(s) 1107
FokI GGATG 1 cut(s) 33
FriOI GRGCYC 1 cut(s) 250
FspBI CTAG 1 cut(s) 639
GlaI GCGC 2 cut(s) 480, 826
GsaI CCCAGC 1 cut(s) 246
GsuI CTGGAG 1 cut(s) 957
HaeII RGCGCY 1 cut(s) 828
HaeIII GGCC 7 cut(s) 29, 70, 79, 281, 528, 793, 893
HgaI GACGC 2 cut(s) 29, 223
HhaI GCGC 2 cut(s) 481, 827
Hin1I GRCGYC 1 cut(s) 40
Hin1II CATG 8 cut(s) 124, 170, 257, 275, 485, 616, 637, 947
Hin6I GCGC 2 cut(s) 479, 825
HinP1I GCGC 2 cut(s) 479, 825
HinfI GANTC 6 cut(s) 18, 196, 204, 460, 499, 599
HphI GGTGA 1 cut(s) 102
Hpy166II GTNNAC 1 cut(s) 812
Hpy188I TCNGA 3 cut(s) 201, 298, 474
Hpy188III TCNNGA 4 cut(s) 464, 936, 1080, 1155
Hpy8I GTNNAC 1 cut(s) 812
Hpy99I CGWCG 1 cut(s) 217
HpyAV CCTTC 1 cut(s) 882
HpyCH4III ACNGT 1 cut(s) 1077
HpyCH4IV ACGT 2 cut(s) 397, 742
HpyF3I CTNAG 7 cut(s) 192, 227, 249, 276, 351, 530, 870
HpySE526I ACGT 2 cut(s) 397, 742
Hsp92I GRCGYC 1 cut(s) 40
Hsp92II CATG 8 cut(s) 124, 170, 257, 275, 485, 616, 637, 947
HspAI GCGC 2 cut(s) 479, 825
KflI GGGWCCC 1 cut(s) 53
KpnI GGTACC 2 cut(s) 87, 1181
Kzo9I GATC 3 cut(s) 304, 976, 1045
LmnI GCTCC 5 cut(s) 145, 235, 382, 466, 1147
LweI GCATC 6 cut(s) 165, 892, 919, 946, 991, 1060
MabI ACCWGGT 1 cut(s) 1012
MaeI CTAG 1 cut(s) 639
MaeII ACGT 2 cut(s) 397, 742
MaeIII GTNAC 2 cut(s) 104, 628
MalI GATC 3 cut(s) 306, 978, 1047
MbiI CCGCTC 1 cut(s) 1142
MboI GATC 3 cut(s) 304, 976, 1045
MboII GAAGA 3 cut(s) 631, 705, 728
MhlI GDGCHC 2 cut(s) 250, 814
MluCI AATT 3 cut(s) 359, 578, 1132
MlyI GAGTC 2 cut(s) 198, 469
MmeI TCCRAC 1 cut(s) 1073
MroXI GAANNNNTTC 1 cut(s) 603
MseI TTAA 2 cut(s) 375, 1062
MspA1I CMGCKG 1 cut(s) 191
MvaI CCWGG 7 cut(s) 75, 681, 737, 795, 876, 969, 1014
NciI CCSGG 4 cut(s) 51, 81, 99, 283
NcoI CCATGG 1 cut(s) 633
NdeI CATATG 1 cut(s) 1107
NdeII GATC 3 cut(s) 304, 976, 1045
NlaIII CATG 8 cut(s) 124, 170, 257, 275, 485, 616, 637, 947
NlaIV GGNNCC 6 cut(s) 54, 55, 85, 991, 1179, 1198
NmeAIII GCCGAG 1 cut(s) 427
NspI RCATGY 5 cut(s) 124, 170, 275, 485, 947
PaeI GCATGC 1 cut(s) 275
PaeR7I CTCGAG 2 cut(s) 1155, 1188
PasI CCCWGGG 1 cut(s) 736
PciI ACATGT 2 cut(s) 120, 943
PdmI GAANNNNTTC 1 cut(s) 603
PfeI GAWTC 4 cut(s) 18, 196, 499, 599
PflMI CCANNNNNTGG 1 cut(s) 1055
PfoI TCCNGGA 1 cut(s) 49
PleI GAGTC 2 cut(s) 198, 468
PmaCI CACGTG 1 cut(s) 398
PmlI CACGTG 1 cut(s) 398
PpsI GAGTC 2 cut(s) 198, 468
Ppu21I YACGTR 1 cut(s) 398
PpuMI RGGWCCY 3 cut(s) 53, 914, 989
PscI ACATGT 2 cut(s) 120, 943
Psp5II RGGWCCY 3 cut(s) 53, 914, 989
Psp6I CCWGG 7 cut(s) 73, 679, 735, 793, 874, 967, 1012
PspCI CACGTG 1 cut(s) 398
PspFI CCCAGC 1 cut(s) 242
PspGI CCWGG 7 cut(s) 73, 679, 735, 793, 874, 967, 1012
PspN4I GGNNCC 6 cut(s) 54, 55, 85, 991, 1179, 1198
PspPPI RGGWCCY 3 cut(s) 53, 914, 989
PspXI VCTCGAGB 1 cut(s) 1188
PstI CTGCAG 3 cut(s) 264, 390, 838
PstNI CAGNNNCTG 6 cut(s) 154, 184, 238, 268, 371, 842
PvuII CAGCTG 1 cut(s) 191
RsaI GTAC 3 cut(s) 85, 584, 1179
RsaNI GTAC 3 cut(s) 84, 583, 1178
SaqAI TTAA 2 cut(s) 375, 1062
Sau3AI GATC 3 cut(s) 304, 976, 1045
SchI GAGTC 2 cut(s) 198, 469
SduI GDGCHC 2 cut(s) 250, 814
SexAI ACCWGGT 1 cut(s) 1012
SfaNI GCATC 6 cut(s) 165, 892, 919, 946, 991, 1060
SfcI CTRYAG 3 cut(s) 260, 386, 834
SfiI GGCCNNNNNGGCC 1 cut(s) 76
Sfr274I CTCGAG 2 cut(s) 1155, 1188
SinI GGWCC 4 cut(s) 53, 914, 989, 1094
SlaI CTCGAG 2 cut(s) 1155, 1188
SmlI CTYRAG 4 cut(s) 521, 1098, 1155, 1188
SmoI CTYRAG 4 cut(s) 521, 1098, 1155, 1188
SphI GCATGC 1 cut(s) 275
Sse9I AATT 3 cut(s) 359, 578, 1132
SsiI CCGC 5 cut(s) 88, 134, 980, 1140, 1145
SspMI CTAG 1 cut(s) 639
StyI CCWWGG 2 cut(s) 633, 1054
TaaI ACNGT 1 cut(s) 1077
TaiI ACGT 2 cut(s) 400, 745
TaqI TCGA 5 cut(s) 16, 212, 597, 1156, 1189
TasI AATT 3 cut(s) 359, 578, 1132
TatI WGTACW 1 cut(s) 582
TauI GCSGC 1 cut(s) 1142
TfiI GAWTC 4 cut(s) 18, 196, 499, 599
Tru1I TTAA 2 cut(s) 375, 1062
Tru9I TTAA 2 cut(s) 375, 1062
TscAI CASTG 3 cut(s) 793, 808, 847
TspDTI ATGAA 3 cut(s) 305, 585, 729
TspRI CASTG 3 cut(s) 793, 808, 847
Van91I CCANNNNNTGG 1 cut(s) 1055
VneI GTGCAC 1 cut(s) 810
VpaK11BI GGWCC 4 cut(s) 53, 914, 989, 1094
XagI CCTNNNNNAGG 3 cut(s) 230, 533, 1191
XceI RCATGY 5 cut(s) 124, 170, 275, 485, 947
XhoI CTCGAG 2 cut(s) 1155, 1188
XmnI GAANNNNTTC 1 cut(s) 603
XspI CTAG 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.