AT1G67170

FLX-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011129
Physical Location & Seq
Forward (+)
25127173 .. 25129464
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G67170.2

Sequence Viewer

Length: 1080 bp
ATGGAAAGCAAAGGAAGAATCCATCCATCTCATCATCATATGAGGCGTCCTCTTCCAGGTCCCGGTGGCTGTATAGCGCATCCGGAGACTTTCGGTAATCACGGTGCTATACCACCTTCTGCTGCTCAAGGTGTGTATCCTTCCTTCAACATGTTACCTCCACCTGAAGTTATGGAGCAAAAGTTTGTGGCACAACACGGGGAATTACAGAGACTTGCTATAGAGAATCAGAGACTTGGTGGAACTCATGGTAGTTTAAGACAAGAGTTAGCAGCAGCACAGCATGAAATACAGATGTTGCACGCGCAAATTGGGTCGATGAAGTCCGAGAGAGAGCAACGGATGATGGGTCTTGCTGAGAAAGTTGCTAAAATGGAGACTGAGCTTCAGAAATCTGAGGCTGTTAAGTTGGAGATGCAACAAGCACGTGCTGAGGCACGGAGTCTTGTTGTGGCTAGGGAGGAGCTTATGTCTAAAGTGCATCAGTTGACTCAGGAACTTCAAAAATCTCGTTCTGATGTGCAGCAAATACCTGCTCTGATGTCTGAACTTGAGAATCTAAGACAGGAGTACCAGCAGTGCAGGGCAACATATGACTATGAGAAGAAGTTTTATAATGACCATCTCGAGTCACTTCAGGCAATGGAGAAGAACTACATGACTATGGCTAGGGAAGTTGAAAAACTTCAAGCACAGTTGATGAACAATGCAAATTCAGATAGAAGAGCAGGTGGCCCTTATGGTAACAACATAAATGCTGAAATTGACGCTTCTGGACATCAGAGTGGAAACGGTTATTATGAAGATGCTTTTGGTCCTCAGGGATATATTCCTCAACCAGTAGCTGGTAACGCAACTGGACCAAATTCAGTTGTTGGCGCAGCTCAATACCCTTATCAAGGAGTAACTCAGCCAGGATACTTCCCTCAAAGACCCGGTTACAACTTTCCAAGAGGCCCTCCTGGTTCATATGACCCAACAACAAGGTTACCAACAGGACCTTACGGCGCTCCATTCCCACCTGGACCATCTAACAATACTCCTTACGCCGGTACACACGGAAACCCTAGTCGCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

359

Amino Acids

39.74

Weight (kDa)

6.93

Isoelectric Point (pI)

52.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011129)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67170 AT1G67170
fragaria_vesca FvH4_6g45670 FvH4_6g45670 FvH4_6g45670
malus_domestica MD09G1080900.v1.1 MD17G1072000.v1.1
prunus_persica Prupe.3G242800_v2.0.a1
pyrus_communis pycom09g00730 pycom17g07130
rosa_chinensis RchiOBHm_Chr2g0163821
rosa_laevigata RLG00000021418
rosa_multiflora Rmu_sc0001181.1_g000004
rosa_roxburghii Rroxscaffold_2G00086990
rosa_rugosa Rorug02G0504800
rosa_samantha Rh2AG572700 Rh2BG583400 Rh2CG554300 Rh2DG593000
rosa_wichuraiana Rw2G047350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 615
AarI CACCTGC 1 cut(s) 719
Acc36I ACCTGC 2 cut(s) 541, 719
AccB7I CCANNNNNTGG 1 cut(s) 845
AccII CGCG 1 cut(s) 305
AccIII TCCGGA 1 cut(s) 82
AcsI RAATTY 2 cut(s) 712, 865
AcuI CTGAAG 3 cut(s) 186, 371, 620
AcvI CACGTG 1 cut(s) 428
AcyI GRCGYC 1 cut(s) 46
AfaI GTAC 2 cut(s) 572, 1054
AfiI CCNNNNNNNGG 5 cut(s) 56, 62, 845, 899, 1049
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 4 cut(s) 148, 503, 680, 689
AjnI CCWGG 4 cut(s) 55, 913, 961, 1021
AjuI GAANNNNNNNTTGG 2 cut(s) 795, 827
AluBI AGCT 4 cut(s) 385, 466, 845, 884
AluI AGCT 4 cut(s) 385, 466, 845, 884
Alw26I GTCTC 4 cut(s) 80, 205, 226, 371
AlwNI CAGNNNCTG 1 cut(s) 845
Ama87I CYCGRG 1 cut(s) 626
Aor13HI TCCGGA 1 cut(s) 82
AoxI GGCC 2 cut(s) 733, 955
ApeKI GCWGC 5 cut(s) 122, 272, 275, 523, 881
ApoI RAATTY 2 cut(s) 712, 865
Asp700I GAANNNNTTC 1 cut(s) 684
AspLEI GCGC 4 cut(s) 79, 307, 881, 1010
AspS9I GGNCC 7 cut(s) 59, 734, 815, 860, 956, 998, 1025
AsuC2I CCSGG 2 cut(s) 63, 936
AvaI CYCGRG 1 cut(s) 626
AvaII GGWCC 5 cut(s) 59, 815, 860, 998, 1025
AxyI CCTNAGG 1 cut(s) 819
BaeI ACNNNNGTAYC 2 cut(s) 554, 587
BarI GAAGNNNNNNTAC 2 cut(s) 128, 160
BbrPI CACGTG 1 cut(s) 428
BbvCI CCTCAGC 1 cut(s) 432
BbvI GCAGC 5 cut(s) 109, 284, 287, 535, 893
BccI CCATC 5 cut(s) 30, 34, 340, 630, 1036
BceAI ACGGC 1 cut(s) 1021
BciT130I CCWGG 4 cut(s) 57, 915, 963, 1023
BciVI GTATCC 2 cut(s) 147, 911
BcnI CCSGG 2 cut(s) 63, 936
BcoDI GTCTC 4 cut(s) 80, 205, 226, 371
BfaI CTAG 3 cut(s) 456, 669, 1068
BfmI CTRYAG 1 cut(s) 219
BfoI RGCGCY 1 cut(s) 1011
BfuAI ACCTGC 2 cut(s) 541, 719
BfuI GTATCC 2 cut(s) 147, 911
BisI GCNGC 5 cut(s) 123, 273, 276, 524, 882
BlsI GCNGC 5 cut(s) 124, 274, 277, 525, 883
Bme1390I CCNGG 6 cut(s) 57, 63, 915, 936, 963, 1023
Bme18I GGWCC 5 cut(s) 59, 815, 860, 998, 1025
BmeT110I CYCGRG 1 cut(s) 626
BmgT120I GGNCC 7 cut(s) 59, 734, 815, 860, 956, 998, 1025
BmiI GGNNCC 1 cut(s) 61
BmrFI CCNGG 6 cut(s) 57, 63, 915, 936, 963, 1023
BmsI GCATC 4 cut(s) 88, 405, 490, 796
BplI GAGNNNNNCTC 2 cut(s) 34, 66
Bpu10I CCTNAGC 1 cut(s) 432
BpuEI CTTGAG 2 cut(s) 111, 572
BpuMI CCSGG 2 cut(s) 63, 936
BsaAI YACGTR 1 cut(s) 428
BsaHI GRCGYC 1 cut(s) 46
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 455, 485
Bsc4I CCNNNNNNNGG 5 cut(s) 56, 62, 845, 899, 1049
Bse118I RCCGGY 1 cut(s) 1049
Bse1I ACTGG 2 cut(s) 839, 862
Bse21I CCTNAGG 1 cut(s) 819
Bse3DI GCAATG 1 cut(s) 648
BseAI TCCGGA 1 cut(s) 82
BseBI CCWGG 4 cut(s) 57, 915, 963, 1023
BseGI GGATG 3 cut(s) 22, 79, 348
BseLI CCNNNNNNNGG 5 cut(s) 56, 62, 845, 899, 1049
BseMI GCAATG 1 cut(s) 648
BseMII CTCAG 7 cut(s) 348, 372, 387, 423, 506, 833, 923
BseNI ACTGG 2 cut(s) 839, 862
BseRI GAGGAG 1 cut(s) 476
BseXI GCAGC 5 cut(s) 109, 284, 287, 535, 893
BsgI GTGCAG 2 cut(s) 542, 601
Bsh1236I CGCG 1 cut(s) 305
BshFI GGCC 2 cut(s) 735, 957
BsiHKCI CYCGRG 1 cut(s) 626
BsiSI CCGG 4 cut(s) 63, 83, 936, 1050
BslFI GGGAC 1 cut(s) 45
BslI CCNNNNNNNGG 5 cut(s) 56, 62, 845, 899, 1049
BsmAI GTCTC 4 cut(s) 80, 205, 226, 371
BsmFI GGGAC 1 cut(s) 45
BsnI GGCC 2 cut(s) 735, 957
BsoBI CYCGRG 1 cut(s) 626
Bsp13I TCCGGA 1 cut(s) 82
BspANI GGCC 2 cut(s) 735, 957
BspCNI CTCAG 7 cut(s) 349, 373, 388, 424, 505, 832, 922
BspEI TCCGGA 1 cut(s) 82
BspFNI CGCG 1 cut(s) 305
BspLI GGNNCC 1 cut(s) 61
BspMI ACCTGC 2 cut(s) 541, 719
BspQI GCTCTTC 1 cut(s) 718
BsrDI GCAATG 1 cut(s) 648
BsrFI RCCGGY 1 cut(s) 1049
BsrI ACTGG 2 cut(s) 839, 862
BssAI RCCGGY 1 cut(s) 1049
BssNI GRCGYC 1 cut(s) 46
Bst2UI CCWGG 4 cut(s) 57, 915, 963, 1023
Bst4CI ACNGT 3 cut(s) 104, 696, 794
Bst6I CTCTTC 2 cut(s) 57, 718
BstACI GRCGYC 1 cut(s) 46
BstBAI YACGTR 1 cut(s) 428
BstC8I GCNNGC 1 cut(s) 303
BstDEI CTNAG 8 cut(s) 357, 381, 396, 432, 492, 560, 819, 909
BstEII GGTNACC 1 cut(s) 987
BstENI CCTNNNNNAGG 2 cut(s) 54, 897
BstF5I GGATG 3 cut(s) 22, 79, 348
BstFNI CGCG 1 cut(s) 305
BstH2I RGCGCY 1 cut(s) 1011
BstHHI GCGC 4 cut(s) 79, 307, 881, 1010
BstMAI GTCTC 4 cut(s) 80, 205, 226, 371
BstMWI GCNNNNNNNGC 1 cut(s) 851
BstNI CCWGG 4 cut(s) 57, 915, 963, 1023
BstNSI RCATGY 1 cut(s) 154
BstPI GGTNACC 1 cut(s) 987
BstSCI CCNGG 6 cut(s) 55, 61, 913, 934, 961, 1021
BstSFI CTRYAG 1 cut(s) 219
BstUI CGCG 1 cut(s) 305
BstV1I GCAGC 5 cut(s) 109, 284, 287, 535, 893
Bsu36I CCTNAGG 1 cut(s) 819
BsuI GTATCC 2 cut(s) 147, 911
BsuRI GGCC 2 cut(s) 735, 957
BtsCI GGATG 3 cut(s) 22, 79, 348
BtsI GCAGTG 1 cut(s) 584
BtsIMutI CAGTG 1 cut(s) 584
BveI ACCTGC 2 cut(s) 541, 719
Cac8I GCNNGC 1 cut(s) 303
CaiI CAGNNNCTG 1 cut(s) 845
CfoI GCGC 4 cut(s) 79, 307, 881, 1010
Cfr10I RCCGGY 1 cut(s) 1049
Cfr13I GGNCC 7 cut(s) 59, 734, 815, 860, 956, 998, 1025
CseI GACGC 2 cut(s) 35, 776
Csp6I GTAC 2 cut(s) 571, 1053
CviAII CATG 4 cut(s) 151, 248, 284, 658
CviQI GTAC 2 cut(s) 571, 1053
DdeI CTNAG 8 cut(s) 357, 381, 396, 432, 492, 560, 819, 909
Eam1104I CTCTTC 2 cut(s) 57, 718
EarI CTCTTC 2 cut(s) 57, 718
Eco47I GGWCC 5 cut(s) 59, 815, 860, 998, 1025
Eco57I CTGAAG 3 cut(s) 186, 371, 620
Eco72I CACGTG 1 cut(s) 428
Eco81I CCTNAGG 1 cut(s) 819
Eco88I CYCGRG 1 cut(s) 626
Eco91I GGTNACC 1 cut(s) 987
EcoNI CCTNNNNNAGG 2 cut(s) 54, 897
EcoO109I RGGNCCY 3 cut(s) 59, 956, 998
EcoO65I GGTNACC 1 cut(s) 987
EcoRII CCWGG 4 cut(s) 55, 913, 961, 1021
FaeI CATG 4 cut(s) 154, 251, 287, 661
FaqI GGGAC 1 cut(s) 45
FatI CATG 4 cut(s) 150, 247, 283, 657
FauNDI CATATG 3 cut(s) 39, 592, 970
Fnu4HI GCNGC 5 cut(s) 123, 273, 276, 524, 882
FokI GGATG 3 cut(s) 9, 66, 355
Fsp4HI GCNGC 5 cut(s) 123, 273, 276, 524, 882
FspBI CTAG 3 cut(s) 456, 669, 1068
GlaI GCGC 4 cut(s) 78, 306, 880, 1009
GluI GCNGC 5 cut(s) 123, 273, 276, 524, 882
HaeII RGCGCY 1 cut(s) 1011
HaeIII GGCC 2 cut(s) 735, 957
HapII CCGG 4 cut(s) 63, 83, 936, 1050
HgaI GACGC 2 cut(s) 35, 776
HhaI GCGC 4 cut(s) 79, 307, 881, 1010
Hin1I GRCGYC 1 cut(s) 46
Hin1II CATG 4 cut(s) 154, 251, 287, 661
Hin6I GCGC 4 cut(s) 77, 305, 879, 1008
HinP1I GCGC 4 cut(s) 77, 305, 879, 1008
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HinfI GANTC 6 cut(s) 18, 226, 442, 490, 556, 629
HpaII CCGG 4 cut(s) 63, 83, 936, 1050
Hpy166II GTNNAC 2 cut(s) 489, 1055
Hpy188I TCNGA 9 cut(s) 231, 328, 390, 397, 517, 540, 547, 718, 783
Hpy188III TCNNGA 4 cut(s) 83, 494, 626, 774
Hpy8I GTNNAC 2 cut(s) 489, 1055
HpyAV CCTTC 3 cut(s) 126, 150, 154
HpyCH4III ACNGT 3 cut(s) 104, 696, 794
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 6 cut(s) 301, 418, 481, 523, 582, 710
HpyF10VI GCNNNNNNNGC 1 cut(s) 851
HpyF3I CTNAG 8 cut(s) 357, 381, 396, 432, 492, 560, 819, 909
HpySE526I ACGT 1 cut(s) 427
Hsp92I GRCGYC 1 cut(s) 46
Hsp92II CATG 4 cut(s) 154, 251, 287, 661
HspAI GCGC 4 cut(s) 77, 305, 879, 1008
Kpn2I TCCGGA 1 cut(s) 82
LguI GCTCTTC 1 cut(s) 718
LmnI GCTCC 3 cut(s) 175, 463, 1015
Lsp1109I GCAGC 5 cut(s) 109, 284, 287, 535, 893
LweI GCATC 4 cut(s) 88, 405, 490, 796
MaeI CTAG 3 cut(s) 456, 669, 1068
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 7 cut(s) 153, 630, 743, 848, 904, 938, 987
MboII GAAGA 6 cut(s) 27, 44, 616, 661, 735, 815
MluCI AATT 5 cut(s) 203, 309, 712, 762, 865
MlyI GAGTC 3 cut(s) 451, 484, 638
MmeI TCCRAC 1 cut(s) 390
MroI TCCGGA 1 cut(s) 82
MroXI GAANNNNTTC 1 cut(s) 684
MseI TTAA 2 cut(s) 257, 405
MslI CAYNNNNRTG 2 cut(s) 662, 783
MspI CCGG 4 cut(s) 63, 83, 936, 1050
MspR9I CCNGG 6 cut(s) 57, 63, 915, 936, 963, 1023
MvaI CCWGG 4 cut(s) 57, 915, 963, 1023
MvnI CGCG 1 cut(s) 305
MwoI GCNNNNNNNGC 1 cut(s) 851
NciI CCSGG 2 cut(s) 63, 936
NdeI CATATG 3 cut(s) 39, 592, 970
NlaIII CATG 4 cut(s) 154, 251, 287, 661
NlaIV GGNNCC 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 630
NspI RCATGY 1 cut(s) 154
PaeR7I CTCGAG 1 cut(s) 626
PaqCI CACCTGC 1 cut(s) 719
PciI ACATGT 1 cut(s) 150
PciSI GCTCTTC 1 cut(s) 718
PdmI GAANNNNTTC 1 cut(s) 684
PfeI GAWTC 3 cut(s) 18, 226, 556
PflMI CCANNNNNTGG 1 cut(s) 845
PkrI GCNGC 5 cut(s) 124, 274, 277, 525, 883
PleI GAGTC 3 cut(s) 450, 484, 637
PmaCI CACGTG 1 cut(s) 428
PmlI CACGTG 1 cut(s) 428
PpsI GAGTC 3 cut(s) 450, 484, 637
Ppu21I YACGTR 1 cut(s) 428
PpuMI RGGWCCY 2 cut(s) 59, 998
PscI ACATGT 1 cut(s) 150
PsiI TTATAA 1 cut(s) 615
Psp5II RGGWCCY 2 cut(s) 59, 998
Psp6I CCWGG 4 cut(s) 55, 913, 961, 1021
PspCI CACGTG 1 cut(s) 428
PspEI GGTNACC 1 cut(s) 987
PspGI CCWGG 4 cut(s) 55, 913, 961, 1021
PspN4I GGNNCC 1 cut(s) 61
PspPI GGNCC 7 cut(s) 59, 734, 815, 860, 956, 998, 1025
PspPPI RGGWCCY 2 cut(s) 59, 998
PstNI CAGNNNCTG 1 cut(s) 845
RsaI GTAC 2 cut(s) 572, 1054
RsaNI GTAC 2 cut(s) 571, 1053
RseI CAYNNNNRTG 2 cut(s) 662, 783
SapI GCTCTTC 1 cut(s) 718
SaqAI TTAA 2 cut(s) 257, 405
SatI GCNGC 5 cut(s) 123, 273, 276, 524, 882
Sau96I GGNCC 7 cut(s) 59, 734, 815, 860, 956, 998, 1025
SchI GAGTC 3 cut(s) 451, 484, 638
ScrFI CCNGG 6 cut(s) 57, 63, 915, 936, 963, 1023
SfaNI GCATC 4 cut(s) 88, 405, 490, 796
SfcI CTRYAG 1 cut(s) 219
Sfr274I CTCGAG 1 cut(s) 626
SinI GGWCC 5 cut(s) 59, 815, 860, 998, 1025
SlaI CTCGAG 1 cut(s) 626
SmiMI CAYNNNNRTG 2 cut(s) 662, 783
SmlI CTYRAG 3 cut(s) 126, 551, 626
SmoI CTYRAG 3 cut(s) 126, 551, 626
Sse9I AATT 5 cut(s) 203, 309, 712, 762, 865
SspMI CTAG 3 cut(s) 456, 669, 1068
StyD4I CCNGG 6 cut(s) 55, 61, 913, 934, 961, 1021
TaaI ACNGT 3 cut(s) 104, 696, 794
TaiI ACGT 1 cut(s) 430
TaqI TCGA 2 cut(s) 317, 627
TasI AATT 5 cut(s) 203, 309, 712, 762, 865
TfiI GAWTC 3 cut(s) 18, 226, 556
Tru1I TTAA 2 cut(s) 257, 405
Tru9I TTAA 2 cut(s) 257, 405
TscAI CASTG 1 cut(s) 584
TseFI GTSAC 1 cut(s) 630
TseI GCWGC 5 cut(s) 122, 272, 275, 523, 881
Tsp45I GTSAC 1 cut(s) 630
TspDTI ATGAA 5 cut(s) 300, 335, 716, 816, 957
TspGWI ACGGA 3 cut(s) 355, 454, 1074
TspRI CASTG 1 cut(s) 584
Van91I CCANNNNNTGG 1 cut(s) 845
VpaK11BI GGWCC 5 cut(s) 59, 815, 860, 998, 1025
XagI CCTNNNNNAGG 2 cut(s) 54, 897
XapI RAATTY 2 cut(s) 712, 865
XceI RCATGY 1 cut(s) 154
XhoI CTCGAG 1 cut(s) 626
XmnI GAANNNNTTC 1 cut(s) 684
XspI CTAG 3 cut(s) 456, 669, 1068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.