Prupe.3G242800_v2.0.a1

FLX-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0011129
Physical Location & Seq
Reverse (-)
23571455 .. 23574523
3069 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G242800.1

Sequence Viewer

Length: 1239 bp
ATGGGAAGCAAAGGTCGAATTCCACCTGCTCATCTTCGTCGACCACTTCCCGGACCCGACCCGTTTGGTCCGGGCATACGCCCGCCCCATGGTGCCTATCCTCCATTTGATATGTTGCCTCCTCCACAAGTTATGGAGCAGAAGTTGGCTGCACAACATGTCGAGATGGAAAGGCTTGTAACTGAGAACCAGAGACTTGCTGCCACACATGGAACCTTGAGACAAGAACTGGCAGGTGCTCAACATGAGTTGCAGATATTGCATGCCCAGATAGGTGCTATCAAGTCTGAGAGAGAACAGCAGATGAGGGGCCTTGTGGATAAAATAGCCAAGATGGAAGCTGAGCTGAAGTCAGCAGAGCCTGTTAAGTTGGAGCTACAGCAGGCTGGAGCTGAAGCCCAAAACTTGGTTGTGTCAAGGCAAGAACTCATTGCCAAAGTGCAACAACTCAGTCAGGATCTTCAGAGGGCTCATACAGATGTGCAGCAGATTCCTGCTTTGGTATCACAGCTTGATAGCCTGAGGCAGGAATATCAGCATTGCAGGGCGACTTATGACTACGAAAAGAAATTATACAATGACCACCTTGAATCGCTTCAGGTCATGGAGAAGAACTATGTTACCATGGCTAGGGAGGTGGAAAAGCTCCGAGCAGAGTTGCTGAATAATTCTAATGTTGATAGAAGAACTGGTGCTCCTTATTATGGAACTCCTGGAAATAATGAAAATGAGTCTACTGGGCACGCAATAGGACAAAATGCTTATGAAGATAGCTATGGTGTTCCCCAGGGACGTGCCCCCTTCCCTCCTCCTGCTGCTGCTGCAGGTGGTGGTGCTACAGCTGCTGGAACTCCTCCATTAGTGGGAGCTCAACCTGGACCACCTGGAAGGACTGTTTATGATGCACCAAGAGGGCCTGGTTATGATGCCTCTGGAGGATCTGGTTATGATGCACAGAGAGGACCTGGTTATGATGCACAAAGAGGAGCTCCCTATGATGCACAGAGACCTGGATATGATGTACAAAGACCAAATTATGAAGTGCAAAGAGGACCCGGTTACGATCCATCCCGAGGTGTCAACTATGATGCACAGTCTAGAGGTGCTGCTGGTCCTCAAGGACATATGCCTACAAGCAATGCGCCTTATGGTTCCGCAACACCACCTGCTCGTGGTGGAAGTGGGTATGAGGCACCACCACCACGAGGAGGGGGAGGGGGCAACCCTGTTCGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

413

Amino Acids

44.31

Weight (kDa)

6.85

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011129)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67170 AT1G67170
fragaria_vesca FvH4_6g45670 FvH4_6g45670 FvH4_6g45670
malus_domestica MD09G1080900.v1.1 MD17G1072000.v1.1
prunus_persica Prupe.3G242800_v2.0.a1
pyrus_communis pycom09g00730 pycom17g07130
rosa_chinensis RchiOBHm_Chr2g0163821
rosa_laevigata RLG00000021418
rosa_multiflora Rmu_sc0001181.1_g000004
rosa_roxburghii Rroxscaffold_2G00086990
rosa_rugosa Rorug02G0504800
rosa_samantha Rh2AG572700 Rh2BG583400 Rh2CG554300 Rh2DG593000
rosa_wichuraiana Rw2G047350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 4 cut(s) 34, 224, 815, 1174
Acc36I ACCTGC 4 cut(s) 34, 224, 815, 1174
AccB1I GGYRCC 2 cut(s) 92, 1192
AccB7I CCANNNNNTGG 1 cut(s) 406
AccI GTMKAC 2 cut(s) 40, 734
AciI CCGC 2 cut(s) 83, 1155
AclWI GGATC 3 cut(s) 465, 946, 1058
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 4 cut(s) 368, 414, 446, 581
AfaI GTAC 1 cut(s) 1023
AflIII ACRYGT 1 cut(s) 157
AgsI TTSAA 1 cut(s) 590
AjiI CACGTC 1 cut(s) 794
AjnI CCWGG 7 cut(s) 712, 786, 874, 883, 916, 964, 1009
AloI GAACNNNNNNTCC 2 cut(s) 679, 711
Alw21I GWGCWC 4 cut(s) 241, 697, 871, 991
Alw26I GTCTC 3 cut(s) 187, 214, 1000
AlwI GGATC 3 cut(s) 465, 946, 1058
AlwNI CAGNNNCTG 2 cut(s) 362, 845
Ama87I CYCGRG 1 cut(s) 1071
AoxI GGCC 2 cut(s) 310, 914
ApeKI GCWGC 8 cut(s) 149, 200, 484, 815, 818, 821, 842, 1106
ApoI RAATTY 1 cut(s) 18
Asp700I GAANNNNTTC 1 cut(s) 594
AspLEI GCGC 1 cut(s) 1144
AspS9I GGNCC 8 cut(s) 53, 68, 310, 878, 914, 962, 1052, 1112
AsuC2I CCSGG 3 cut(s) 51, 72, 1056
AsuII TTCGAA 1 cut(s) 1231
AvaI CYCGRG 1 cut(s) 1071
AvaII GGWCC 6 cut(s) 53, 68, 878, 962, 1052, 1112
AxyI CCTNAGG 1 cut(s) 521
BaeGI GKGCMC 2 cut(s) 744, 799
BanI GGYRCC 2 cut(s) 92, 1192
BanII GRGCYC 3 cut(s) 472, 871, 991
BauI CACGAG 2 cut(s) 1170, 1203
Bbv12I GWGCWC 4 cut(s) 241, 697, 871, 991
BbvI GCAGC 8 cut(s) 136, 187, 496, 802, 805, 808, 829, 1093
BccI CCATC 3 cut(s) 160, 328, 1075
BciT130I CCWGG 7 cut(s) 714, 788, 876, 885, 918, 966, 1011
BcnI CCSGG 3 cut(s) 51, 72, 1056
BcoDI GTCTC 3 cut(s) 187, 214, 1000
BfaI CTAG 2 cut(s) 630, 1098
BfmI CTRYAG 3 cut(s) 377, 822, 837
BfuAI ACCTGC 4 cut(s) 34, 224, 815, 1174
BisI GCNGC 8 cut(s) 150, 201, 485, 816, 819, 822, 843, 1107
BlpI GCTNAGC 1 cut(s) 342
BlsI GCNGC 8 cut(s) 151, 202, 486, 817, 820, 823, 844, 1108
Bme18I GGWCC 6 cut(s) 53, 68, 878, 962, 1052, 1112
BmeT110I CYCGRG 1 cut(s) 1071
BmgBI CACGTC 1 cut(s) 794
BmgT120I GGNCC 8 cut(s) 53, 68, 310, 878, 914, 962, 1052, 1112
BmiI GGNNCC 7 cut(s) 55, 94, 214, 311, 1054, 1153, 1194
BmrI ACTGGG 1 cut(s) 747
BmsI GCATC 6 cut(s) 892, 916, 940, 964, 988, 1078
BmuI ACTGGG 1 cut(s) 747
BpmI CTGGAG 2 cut(s) 408, 954
Bpu1102I GCTNAGC 1 cut(s) 342
Bpu14I TTCGAA 1 cut(s) 1231
BpuEI CTTGAG 2 cut(s) 238, 1101
BpuMI CCSGG 3 cut(s) 51, 72, 1056
BsaI GGTCTC 1 cut(s) 1000
BsaJI CCNNGG 5 cut(s) 88, 624, 786, 787, 1072
BsaXI ACNNNNNCTCC 4 cut(s) 128, 158, 679, 709
Bse1I ACTGG 3 cut(s) 234, 694, 742
Bse21I CCTNAGG 1 cut(s) 521
Bse3DI GCAATG 3 cut(s) 429, 538, 1144
BseBI CCWGG 7 cut(s) 714, 788, 876, 885, 918, 966, 1011
BseDI CCNNGG 5 cut(s) 88, 624, 786, 787, 1072
BseGI GGATG 1 cut(s) 1067
BseMI GCAATG 3 cut(s) 429, 538, 1144
BseMII CTCAG 5 cut(s) 174, 279, 333, 463, 512
BseNI ACTGG 3 cut(s) 234, 694, 742
BseRI GAGGAG 5 cut(s) 111, 798, 843, 999, 1221
BseSI GKGCMC 2 cut(s) 744, 799
BseXI GCAGC 8 cut(s) 136, 187, 496, 802, 805, 808, 829, 1093
BsgI GTGCAG 2 cut(s) 135, 503
BshFI GGCC 2 cut(s) 312, 916
BshNI GGYRCC 2 cut(s) 92, 1192
BsiHKAI GWGCWC 4 cut(s) 241, 697, 871, 991
BsiHKCI CYCGRG 1 cut(s) 1071
BsiSI CCGG 3 cut(s) 51, 71, 1056
BslFI GGGAC 1 cut(s) 804
BsmAI GTCTC 3 cut(s) 187, 214, 1000
BsmFI GGGAC 1 cut(s) 804
BsnI GGCC 2 cut(s) 312, 916
Bso31I GGTCTC 1 cut(s) 1000
BsoBI CYCGRG 1 cut(s) 1071
Bsp119I TTCGAA 1 cut(s) 1231
Bsp1286I GDGCHC 7 cut(s) 241, 472, 697, 744, 799, 871, 991
Bsp1407I TGTACA 1 cut(s) 1021
Bsp143I GATC 3 cut(s) 457, 938, 1063
Bsp1720I GCTNAGC 1 cut(s) 342
Bsp19I CCATGG 2 cut(s) 88, 624
BspACI CCGC 2 cut(s) 83, 1155
BspANI GGCC 2 cut(s) 312, 916
BspCNI CTCAG 5 cut(s) 175, 280, 334, 462, 513
BspLI GGNNCC 7 cut(s) 55, 94, 214, 311, 1054, 1153, 1194
BspMAI CTGCAG 1 cut(s) 826
BspMI ACCTGC 4 cut(s) 34, 224, 815, 1174
BspPI GGATC 3 cut(s) 465, 946, 1058
BspT104I TTCGAA 1 cut(s) 1231
BspT107I GGYRCC 2 cut(s) 92, 1192
BspTNI GGTCTC 1 cut(s) 1000
BsrDI GCAATG 3 cut(s) 429, 538, 1144
BsrGI TGTACA 1 cut(s) 1021
BsrI ACTGG 3 cut(s) 234, 694, 742
BssECI CCNNGG 5 cut(s) 88, 624, 786, 787, 1072
BssMI GATC 3 cut(s) 457, 938, 1063
BssSI CACGAG 2 cut(s) 1170, 1203
BssT1I CCWWGG 2 cut(s) 88, 624
Bst2BI CACGAG 2 cut(s) 1170, 1203
Bst2UI CCWGG 7 cut(s) 714, 788, 876, 885, 918, 966, 1011
Bst4CI ACNGT 2 cut(s) 895, 1095
BstAPI GCANNNNNTGC 1 cut(s) 259
BstAUI TGTACA 1 cut(s) 1021
BstBI TTCGAA 1 cut(s) 1231
BstC8I GCNNGC 4 cut(s) 83, 264, 384, 744
BstDEI CTNAG 5 cut(s) 183, 288, 342, 449, 521
BstDSI CCRYGG 2 cut(s) 88, 624
BstENI CCTNNNNNAGG 1 cut(s) 524
BstF5I GGATG 1 cut(s) 1067
BstHHI GCGC 1 cut(s) 1144
BstKTI GATC 3 cut(s) 460, 941, 1066
BstMAI GTCTC 3 cut(s) 187, 214, 1000
BstMBI GATC 3 cut(s) 457, 938, 1063
BstMWI GCNNNNNNNGC 3 cut(s) 259, 821, 842
BstNI CCWGG 7 cut(s) 714, 788, 876, 885, 918, 966, 1011
BstNSI RCATGY 2 cut(s) 161, 266
BstSFI CTRYAG 3 cut(s) 377, 822, 837
BstSLI GKGCMC 2 cut(s) 744, 799
BstV1I GCAGC 8 cut(s) 136, 187, 496, 802, 805, 808, 829, 1093
BstX2I RGATCY 2 cut(s) 457, 938
BstYI RGATCY 2 cut(s) 457, 938
Bsu36I CCTNAGG 1 cut(s) 521
BsuRI GGCC 2 cut(s) 312, 916
BtgI CCRYGG 2 cut(s) 88, 624
BtrI CACGTC 1 cut(s) 794
BtsCI GGATG 1 cut(s) 1067
BveI ACCTGC 4 cut(s) 34, 224, 815, 1174
Cac8I GCNNGC 4 cut(s) 83, 264, 384, 744
CaiI CAGNNNCTG 2 cut(s) 362, 845
CfoI GCGC 1 cut(s) 1144
Cfr13I GGNCC 8 cut(s) 53, 68, 310, 878, 914, 962, 1052, 1112
CsiI ACCWGGT 1 cut(s) 964
Csp6I GTAC 1 cut(s) 1022
CviAII CATG 7 cut(s) 89, 158, 209, 245, 263, 604, 625
CviQI GTAC 1 cut(s) 1022
DdeI CTNAG 5 cut(s) 183, 288, 342, 449, 521
DpnI GATC 3 cut(s) 459, 940, 1065
DpnII GATC 3 cut(s) 457, 938, 1063
Ecl136II GAGCTC 2 cut(s) 869, 989
Eco130I CCWWGG 2 cut(s) 88, 624
Eco24I GRGCYC 3 cut(s) 472, 871, 991
Eco31I GGTCTC 1 cut(s) 1000
Eco47I GGWCC 6 cut(s) 53, 68, 878, 962, 1052, 1112
Eco53kI GAGCTC 2 cut(s) 869, 989
Eco57I CTGAAG 4 cut(s) 368, 414, 446, 581
Eco81I CCTNAGG 1 cut(s) 521
Eco88I CYCGRG 1 cut(s) 1071
EcoICRI GAGCTC 2 cut(s) 869, 989
EcoNI CCTNNNNNAGG 1 cut(s) 524
EcoO109I RGGNCCY 4 cut(s) 310, 914, 962, 1052
EcoRI GAATTC 1 cut(s) 18
EcoRII CCWGG 7 cut(s) 712, 786, 874, 883, 916, 964, 1009
EcoT14I CCWWGG 2 cut(s) 88, 624
EcoT38I GRGCYC 3 cut(s) 472, 871, 991
ErhI CCWWGG 2 cut(s) 88, 624
FaeI CATG 7 cut(s) 92, 161, 212, 248, 266, 607, 628
FaqI GGGAC 1 cut(s) 804
FatI CATG 7 cut(s) 88, 157, 208, 244, 262, 603, 624
FauI CCCGC 1 cut(s) 90
FauNDI CATATG 1 cut(s) 1125
FblI GTMKAC 2 cut(s) 40, 734
Fnu4HI GCNGC 8 cut(s) 150, 201, 485, 816, 819, 822, 843, 1107
FokI GGATG 1 cut(s) 1054
FriOI GRGCYC 3 cut(s) 472, 871, 991
Fsp4HI GCNGC 8 cut(s) 150, 201, 485, 816, 819, 822, 843, 1107
FspBI CTAG 2 cut(s) 630, 1098
GlaI GCGC 1 cut(s) 1143
GluI GCNGC 8 cut(s) 150, 201, 485, 816, 819, 822, 843, 1107
GsuI CTGGAG 2 cut(s) 408, 954
HaeIII GGCC 2 cut(s) 312, 916
HapII CCGG 3 cut(s) 51, 71, 1056
HhaI GCGC 1 cut(s) 1144
Hin1II CATG 7 cut(s) 92, 161, 212, 248, 266, 607, 628
Hin6I GCGC 1 cut(s) 1142
HinP1I GCGC 1 cut(s) 1142
HincII GTYRAC 2 cut(s) 41, 1081
HindII GTYRAC 2 cut(s) 41, 1081
HinfI GANTC 3 cut(s) 490, 590, 731
HpaII CCGG 3 cut(s) 51, 71, 1056
Hpy166II GTNNAC 3 cut(s) 41, 735, 1081
Hpy188I TCNGA 3 cut(s) 289, 465, 650
Hpy188III TCNNGA 5 cut(s) 163, 455, 933, 1071, 1098
Hpy8I GTNNAC 3 cut(s) 41, 735, 1081
Hpy99I CGWCG 1 cut(s) 42
HpyAV CCTTC 2 cut(s) 811, 882
HpyCH4III ACNGT 2 cut(s) 895, 1095
HpyCH4IV ACGT 1 cut(s) 793
HpyF10VI GCNNNNNNNGC 3 cut(s) 259, 821, 842
HpyF3I CTNAG 5 cut(s) 183, 288, 342, 449, 521
HpySE526I ACGT 1 cut(s) 793
Hsp92II CATG 7 cut(s) 92, 161, 212, 248, 266, 607, 628
HspAI GCGC 1 cut(s) 1142
Kzo9I GATC 3 cut(s) 457, 938, 1063
LmnI GCTCC 8 cut(s) 136, 373, 389, 651, 700, 866, 986, 994
Lsp1109I GCAGC 8 cut(s) 136, 187, 496, 802, 805, 808, 829, 1093
LweI GCATC 6 cut(s) 892, 916, 940, 964, 988, 1078
MabI ACCWGGT 1 cut(s) 964
MaeI CTAG 2 cut(s) 630, 1098
MaeII ACGT 1 cut(s) 793
MaeIII GTNAC 3 cut(s) 178, 619, 1058
MalI GATC 3 cut(s) 459, 940, 1065
MboI GATC 3 cut(s) 457, 938, 1063
MboII GAAGA 5 cut(s) 26, 452, 622, 696, 779
MflI RGATCY 2 cut(s) 457, 938
MhlI GDGCHC 7 cut(s) 241, 472, 697, 744, 799, 871, 991
MluCI AATT 4 cut(s) 18, 569, 667, 1033
MlyI GAGTC 1 cut(s) 740
MmeI TCCRAC 1 cut(s) 351
MroXI GAANNNNTTC 1 cut(s) 594
MseI TTAA 1 cut(s) 366
MslI CAYNNNNRTG 1 cut(s) 477
MspA1I CMGCKG 1 cut(s) 842
MspI CCGG 3 cut(s) 51, 71, 1056
MvaI CCWGG 7 cut(s) 714, 788, 876, 885, 918, 966, 1011
MwoI GCNNNNNNNGC 3 cut(s) 259, 821, 842
NciI CCSGG 3 cut(s) 51, 72, 1056
NcoI CCATGG 2 cut(s) 88, 624
NdeI CATATG 1 cut(s) 1125
NdeII GATC 3 cut(s) 457, 938, 1063
NlaIII CATG 7 cut(s) 92, 161, 212, 248, 266, 607, 628
NlaIV GGNNCC 7 cut(s) 55, 94, 214, 311, 1054, 1153, 1194
NspI RCATGY 2 cut(s) 161, 266
NspV TTCGAA 1 cut(s) 1231
PaeI GCATGC 1 cut(s) 266
PaqCI CACCTGC 4 cut(s) 34, 224, 815, 1174
PasI CCCWGGG 1 cut(s) 787
PciI ACATGT 1 cut(s) 157
PdmI GAANNNNTTC 1 cut(s) 594
PfeI GAWTC 2 cut(s) 490, 590
PflMI CCANNNNNTGG 1 cut(s) 406
PfoI TCCNGGA 2 cut(s) 49, 712
PkrI GCNGC 8 cut(s) 151, 202, 486, 817, 820, 823, 844, 1108
PleI GAGTC 1 cut(s) 739
PpsI GAGTC 1 cut(s) 739
PpuMI RGGWCCY 2 cut(s) 962, 1052
PscI ACATGT 1 cut(s) 157
Psp124BI GAGCTC 2 cut(s) 871, 991
Psp5II RGGWCCY 2 cut(s) 962, 1052
Psp6I CCWGG 7 cut(s) 712, 786, 874, 883, 916, 964, 1009
PspGI CCWGG 7 cut(s) 712, 786, 874, 883, 916, 964, 1009
PspN4I GGNNCC 7 cut(s) 55, 94, 214, 311, 1054, 1153, 1194
PspPI GGNCC 8 cut(s) 53, 68, 310, 878, 914, 962, 1052, 1112
PspPPI RGGWCCY 2 cut(s) 962, 1052
PstI CTGCAG 1 cut(s) 826
PstNI CAGNNNCTG 2 cut(s) 362, 845
PsuI RGATCY 2 cut(s) 457, 938
PvuII CAGCTG 1 cut(s) 842
RsaI GTAC 1 cut(s) 1023
RsaNI GTAC 1 cut(s) 1022
RseI CAYNNNNRTG 1 cut(s) 477
SacI GAGCTC 2 cut(s) 871, 991
SalI GTCGAC 1 cut(s) 39
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 8 cut(s) 150, 201, 485, 816, 819, 822, 843, 1107
Sau3AI GATC 3 cut(s) 457, 938, 1063
Sau96I GGNCC 8 cut(s) 53, 68, 310, 878, 914, 962, 1052, 1112
SchI GAGTC 1 cut(s) 740
SduI GDGCHC 7 cut(s) 241, 472, 697, 744, 799, 871, 991
SexAI ACCWGGT 1 cut(s) 964
SfaNI GCATC 6 cut(s) 892, 916, 940, 964, 988, 1078
SfcI CTRYAG 3 cut(s) 377, 822, 837
SfuI TTCGAA 1 cut(s) 1231
SinI GGWCC 6 cut(s) 53, 68, 878, 962, 1052, 1112
SmiMI CAYNNNNRTG 1 cut(s) 477
SmlI CTYRAG 2 cut(s) 217, 1116
SmoI CTYRAG 2 cut(s) 217, 1116
SphI GCATGC 1 cut(s) 266
Sse9I AATT 4 cut(s) 18, 569, 667, 1033
SsiI CCGC 2 cut(s) 83, 1155
SspMI CTAG 2 cut(s) 630, 1098
SstI GAGCTC 2 cut(s) 871, 991
StyI CCWWGG 2 cut(s) 88, 624
TaaI ACNGT 2 cut(s) 895, 1095
TaiI ACGT 1 cut(s) 796
TaqI TCGA 4 cut(s) 16, 40, 162, 1231
TasI AATT 4 cut(s) 18, 569, 667, 1033
TatI WGTACW 1 cut(s) 1021
TfiI GAWTC 2 cut(s) 490, 590
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TseI GCWGC 8 cut(s) 149, 200, 484, 815, 818, 821, 842, 1106
TspDTI ATGAA 3 cut(s) 738, 780, 1053
Van91I CCANNNNNTGG 1 cut(s) 406
VpaK11BI GGWCC 6 cut(s) 53, 68, 878, 962, 1052, 1112
XagI CCTNNNNNAGG 1 cut(s) 524
XapI RAATTY 1 cut(s) 18
XbaI TCTAGA 1 cut(s) 1097
XceI RCATGY 2 cut(s) 161, 266
XmiI GTMKAC 2 cut(s) 40, 734
XmnI GAANNNNTTC 1 cut(s) 594
XspI CTAG 2 cut(s) 630, 1098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.