Rroxscaffold_2G00086990

FLX-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011129
Physical Location & Seq
Forward (+)
9094906 .. 9097704
2799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00086990.1

Sequence Viewer

Length: 1293 bp
ATGGGAAGTAAAGGTCGAATCCCACCGGCCCATCTCCGGCGTCCTCATCCCGGACCCGACCCGTATGGGCCTGGTGGGCCGGGCACCCGCCCACCTCCGGGTGCTTATTCACCCTTTGACATGTTGCCTCCTCCACAAGTTTTGGAGCAGAAGCTGGCTTCACAGCATGTGGAGATGCAGAGGCTGGCAACAGAGAACCAGAGACTGGCTTCGACGCACGGCAGCCTCAGGCAGGAGCTGGCAGGGGCTCAGCATGAGCTGCAGATACTGCATGCTCAGGCCGGAGCTATGAAGTCGGAGAGGGATCAGCAGATGAGGAGCCTGGCGGATAAGATTGCAAAAATGGAGGCTGAGCTGAAAGCGGCGGAGTCTGTTAAGGTGGAGCTGCAGCAGGTGCGTGCTGAAGCAAATAACTTGGTTGGGGTGAGGCAGGAGAGTATTGTTAAAGTGCAGCAGTTGAGTCAGGATCTTCAGAGGGCGCATGGGGATGTGCAGCAGATTCCAGTTTTGGTTCAGCAACTCGATGGCCTCAGGCAGGAGTATCAGCATTGCAGGGCTACTTTTGACTATGAAAAGAAATTGTACAATGACCACCTTGAATCACTCCAGGTCATGGAGAAGAACTATGTTACCATGGCTAGGGAGGTAGAAAAGCTACGAGCAGAGTTATTGAATAATTCTGGTGCTGATAGAAGAACTGGTGGGTCTTATTATGGGAATCCTGGAACTAATGAAAGTGAGGCTTCTGGGCTTGCTGCAGGACAAAATGCTTATGAAGATAACTATGGTGTTCCCCAGGGACGTGTCCCCTTTCCTCCTACTGCTGGTGGTGGTGGTCAAAGTGGGCCTGGGGGCAGTGCTGGTGCTGCTTCTGGCGGTGCTGCTGCTGCCGCCCCTGCTGGAACTTCTCCGTTTGCTGGATCTCAATCTGGACCTCCTGGAAGGACAGGTTATGATGCACCAAGAGGGCCTGTTGGTTATGATCCCTCTGGAGGATCTGCTTATGATGCACAAAGAACTGGCTATGATCCACAAAGAGGAGCCGTCTATGATCCACAAAGAGGAGCTGTCTATGATGTACATAGGCCAGGTTATGAGGTGCAAAGAGGAACCGGTTATGATCCAACCCGGGGTCTTAACTATGATGCACAGTCCAGAGGTGCTTCTGGTCCTCAGGGACATGTGCCTACAAGCAATATGCCCTATGGTTCAGCCACTCCACCTACTGCTCGAGGAGGAACTGGGTATGAGGCACCGCCACCTCGAGGAGGTGCAAACCCAGTTCGTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

430

Amino Acids

45.6

Weight (kDa)

7.84

Isoelectric Point (pI)

47.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011129)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67170 AT1G67170
fragaria_vesca FvH4_6g45670 FvH4_6g45670 FvH4_6g45670
malus_domestica MD09G1080900.v1.1 MD17G1072000.v1.1
prunus_persica Prupe.3G242800_v2.0.a1
pyrus_communis pycom09g00730 pycom17g07130
rosa_chinensis RchiOBHm_Chr2g0163821
rosa_laevigata RLG00000021418
rosa_multiflora Rmu_sc0001181.1_g000004
rosa_roxburghii Rroxscaffold_2G00086990
rosa_rugosa Rorug02G0504800
rosa_samantha Rh2AG572700 Rh2BG583400 Rh2CG554300 Rh2DG593000
rosa_wichuraiana Rw2G047350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 382
AbsI CCTCGAGG 1 cut(s) 1263
Acc36I ACCTGC 1 cut(s) 382
AccB1I GGYRCC 2 cut(s) 83, 1252
AccB7I CCANNNNNTGG 2 cut(s) 205, 613
AciI CCGC 7 cut(s) 88, 326, 362, 365, 876, 891, 1256
AclWI GGATC 8 cut(s) 312, 474, 928, 977, 1003, 1022, 1046, 1115
AcuI CTGAAG 2 cut(s) 423, 455
AcyI GRCGYC 1 cut(s) 40
AfaI GTAC 2 cut(s) 584, 1080
AflIII ACRYGT 3 cut(s) 120, 802, 1180
AgeI ACCGGT 1 cut(s) 1112
AgsI TTSAA 2 cut(s) 599, 673
AjiI CACGTC 1 cut(s) 803
AjnI CCWGG 8 cut(s) 70, 321, 606, 721, 795, 847, 937, 1087
AluBI AGCT 8 cut(s) 154, 238, 259, 287, 355, 385, 655, 1067
AluI AGCT 8 cut(s) 154, 238, 259, 287, 355, 385, 655, 1067
Alw26I GTCTC 1 cut(s) 196
AlwI GGATC 8 cut(s) 312, 474, 928, 977, 1003, 1022, 1046, 1115
AlwNI CAGNNNCTG 5 cut(s) 154, 184, 205, 238, 268
Ama87I CYCGRG 3 cut(s) 1128, 1230, 1263
AoxI GGCC 8 cut(s) 27, 68, 77, 279, 526, 845, 968, 1085
AsiGI ACCGGT 1 cut(s) 1112
AspLEI GCGC 1 cut(s) 481
AspS9I GGNCC 8 cut(s) 28, 53, 68, 77, 845, 932, 968, 1169
AsuC2I CCSGG 5 cut(s) 51, 81, 99, 1129, 1130
AsuHPI GGTGA 2 cut(s) 102, 436
AvaI CYCGRG 3 cut(s) 1128, 1230, 1263
AvaII GGWCC 3 cut(s) 53, 932, 1169
AxyI CCTNAGG 3 cut(s) 227, 530, 1173
BaeGI GKGCMC 1 cut(s) 86
BanI GGYRCC 2 cut(s) 83, 1252
BanII GRGCYC 1 cut(s) 250
BccI CCATC 2 cut(s) 39, 518
BceAI ACGGC 2 cut(s) 235, 1028
BciT130I CCWGG 8 cut(s) 72, 323, 608, 723, 797, 849, 939, 1089
BcnI CCSGG 5 cut(s) 51, 81, 99, 1129, 1130
BcoDI GTCTC 1 cut(s) 196
BfaI CTAG 1 cut(s) 639
BfmI CTRYAG 3 cut(s) 260, 386, 756
BfuAI ACCTGC 1 cut(s) 382
BglI GCCNNNNNGGC 1 cut(s) 76
BlpI GCTNAGC 2 cut(s) 249, 351
Bme18I GGWCC 3 cut(s) 53, 932, 1169
BmeT110I CYCGRG 3 cut(s) 1128, 1230, 1263
BmgBI CACGTC 1 cut(s) 803
BmgT120I GGNCC 8 cut(s) 28, 53, 68, 77, 845, 932, 968, 1169
BmiI GGNNCC 6 cut(s) 55, 85, 320, 1042, 1111, 1254
BmrI ACTGGG 2 cut(s) 1251, 1274
BmsI GCATC 4 cut(s) 165, 946, 997, 1135
BmuI ACTGGG 2 cut(s) 1251, 1274
BpmI CTGGAG 2 cut(s) 590, 1011
Bpu10I CCTNAGC 1 cut(s) 276
Bpu1102I GCTNAGC 2 cut(s) 249, 351
BpuMI CCSGG 5 cut(s) 51, 81, 99, 1129, 1130
BsaBI GATNNNNATC 1 cut(s) 925
BsaHI GRCGYC 1 cut(s) 40
BsaJI CCNNGG 6 cut(s) 633, 795, 796, 848, 1128, 1129
BsaWI WCCGGW 1 cut(s) 1112
Bse118I RCCGGY 2 cut(s) 25, 1112
Bse1I ACTGG 6 cut(s) 210, 503, 703, 1024, 1246, 1280
Bse21I CCTNAGG 3 cut(s) 227, 530, 1173
Bse3DI GCAATG 1 cut(s) 547
Bse8I GATNNNNATC 1 cut(s) 925
BseBI CCWGG 8 cut(s) 72, 323, 608, 723, 797, 849, 939, 1089
BseDI CCNNGG 6 cut(s) 633, 795, 796, 848, 1128, 1129
BseGI GGATG 2 cut(s) 46, 493
BseJI GATNNNNATC 1 cut(s) 925
BseMI GCAATG 1 cut(s) 547
BseMII CTCAG 6 cut(s) 241, 263, 290, 342, 544, 1187
BseNI ACTGG 6 cut(s) 210, 503, 703, 1024, 1246, 1280
BseRI GAGGAG 6 cut(s) 120, 331, 1053, 1077, 1248, 1281
BseSI GKGCMC 1 cut(s) 86
BsgI GTGCAG 2 cut(s) 470, 512
BshFI GGCC 8 cut(s) 29, 70, 79, 281, 528, 847, 970, 1087
BshNI GGYRCC 2 cut(s) 83, 1252
BshTI ACCGGT 1 cut(s) 1112
BsiHKCI CYCGRG 3 cut(s) 1128, 1230, 1263
BsiSI CCGG 8 cut(s) 26, 37, 51, 80, 98, 282, 1113, 1129
BslFI GGGAC 3 cut(s) 791, 813, 1191
BsmAI GTCTC 1 cut(s) 196
BsmFI GGGAC 3 cut(s) 791, 813, 1191
BsnI GGCC 8 cut(s) 29, 70, 79, 281, 528, 847, 970, 1087
BsoBI CYCGRG 3 cut(s) 1128, 1230, 1263
Bsp1286I GDGCHC 2 cut(s) 86, 250
Bsp1407I TGTACA 2 cut(s) 582, 1078
Bsp143I GATC 8 cut(s) 304, 466, 920, 982, 995, 1027, 1051, 1120
Bsp1720I GCTNAGC 2 cut(s) 249, 351
Bsp19I CCATGG 1 cut(s) 633
BspACI CCGC 7 cut(s) 88, 326, 362, 365, 876, 891, 1256
BspANI GGCC 8 cut(s) 29, 70, 79, 281, 528, 847, 970, 1087
BspCNI CTCAG 6 cut(s) 240, 262, 289, 343, 543, 1186
BspLI GGNNCC 6 cut(s) 55, 85, 320, 1042, 1111, 1254
BspMAI CTGCAG 3 cut(s) 264, 390, 760
BspMI ACCTGC 1 cut(s) 382
BspPI GGATC 8 cut(s) 312, 474, 928, 977, 1003, 1022, 1046, 1115
BspT107I GGYRCC 2 cut(s) 83, 1252
BsrDI GCAATG 1 cut(s) 547
BsrFI RCCGGY 2 cut(s) 25, 1112
BsrGI TGTACA 2 cut(s) 582, 1078
BsrI ACTGG 6 cut(s) 210, 503, 703, 1024, 1246, 1280
BssAI RCCGGY 2 cut(s) 25, 1112
BssECI CCNNGG 6 cut(s) 633, 795, 796, 848, 1128, 1129
BssMI GATC 8 cut(s) 304, 466, 920, 982, 995, 1027, 1051, 1120
BssNI GRCGYC 1 cut(s) 40
BssT1I CCWWGG 1 cut(s) 633
Bst2UI CCWGG 8 cut(s) 72, 323, 608, 723, 797, 849, 939, 1089
Bst4CI ACNGT 1 cut(s) 1152
BstACI GRCGYC 1 cut(s) 40
BstAPI GCANNNNNTGC 3 cut(s) 259, 268, 394
BstAUI TGTACA 2 cut(s) 582, 1078
BstC8I GCNNGC 6 cut(s) 156, 186, 240, 273, 399, 753
BstDEI CTNAG 6 cut(s) 227, 249, 276, 351, 530, 1173
BstDSI CCRYGG 1 cut(s) 633
BstENI CCTNNNNNAGG 3 cut(s) 230, 533, 1266
BstF5I GGATG 2 cut(s) 46, 493
BstHHI GCGC 1 cut(s) 481
BstKTI GATC 8 cut(s) 307, 469, 923, 985, 998, 1030, 1054, 1123
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 8 cut(s) 304, 466, 920, 982, 995, 1027, 1051, 1120
BstMWI GCNNNNNNNGC 9 cut(s) 76, 259, 268, 394, 866, 887, 890, 896, 1007
BstNI CCWGG 8 cut(s) 72, 323, 608, 723, 797, 849, 939, 1089
BstNSI RCATGY 4 cut(s) 124, 170, 275, 1184
BstSFI CTRYAG 3 cut(s) 260, 386, 756
BstSLI GKGCMC 1 cut(s) 86
BstX2I RGATCY 3 cut(s) 466, 920, 995
BstYI RGATCY 3 cut(s) 466, 920, 995
Bsu36I CCTNAGG 3 cut(s) 227, 530, 1173
BsuRI GGCC 8 cut(s) 29, 70, 79, 281, 528, 847, 970, 1087
BtgI CCRYGG 1 cut(s) 633
BtrI CACGTC 1 cut(s) 803
BtsCI GGATG 2 cut(s) 46, 493
BtsI GCAGTG 1 cut(s) 862
BtsIMutI CAGTG 1 cut(s) 862
BveI ACCTGC 1 cut(s) 382
Cac8I GCNNGC 6 cut(s) 156, 186, 240, 273, 399, 753
CaiI CAGNNNCTG 5 cut(s) 154, 184, 205, 238, 268
CfoI GCGC 1 cut(s) 481
Cfr10I RCCGGY 2 cut(s) 25, 1112
Cfr13I GGNCC 8 cut(s) 28, 53, 68, 77, 845, 932, 968, 1169
Cfr9I CCCGGG 1 cut(s) 1128
CseI GACGC 2 cut(s) 29, 223
Csp6I GTAC 2 cut(s) 583, 1079
CspAI ACCGGT 1 cut(s) 1112
CspCI CAANNNNNGTGG 2 cut(s) 123, 158
CviAII CATG 8 cut(s) 121, 167, 254, 272, 482, 613, 634, 1181
CviQI GTAC 2 cut(s) 583, 1079
DdeI CTNAG 6 cut(s) 227, 249, 276, 351, 530, 1173
DpnI GATC 8 cut(s) 306, 468, 922, 984, 997, 1029, 1053, 1122
DpnII GATC 8 cut(s) 304, 466, 920, 982, 995, 1027, 1051, 1120
EciI GGCGGA 2 cut(s) 341, 380
Eco130I CCWWGG 1 cut(s) 633
Eco24I GRGCYC 1 cut(s) 250
Eco47I GGWCC 3 cut(s) 53, 932, 1169
Eco57I CTGAAG 2 cut(s) 423, 455
Eco81I CCTNAGG 3 cut(s) 227, 530, 1173
Eco88I CYCGRG 3 cut(s) 1128, 1230, 1263
EcoNI CCTNNNNNAGG 3 cut(s) 230, 533, 1266
EcoO109I RGGNCCY 1 cut(s) 968
EcoRII CCWGG 8 cut(s) 70, 321, 606, 721, 795, 847, 937, 1087
EcoT14I CCWWGG 1 cut(s) 633
EcoT38I GRGCYC 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 633
FaeI CATG 8 cut(s) 124, 170, 257, 275, 485, 616, 637, 1184
FalI AAGNNNNNCTT 2 cut(s) 727, 759
FaqI GGGAC 3 cut(s) 791, 813, 1191
FatI CATG 8 cut(s) 120, 166, 253, 271, 481, 612, 633, 1180
FauI CCCGC 1 cut(s) 95
FokI GGATG 2 cut(s) 33, 500
FriOI GRGCYC 1 cut(s) 250
FspBI CTAG 1 cut(s) 639
GlaI GCGC 1 cut(s) 480
GsuI CTGGAG 2 cut(s) 590, 1011
HaeIII GGCC 8 cut(s) 29, 70, 79, 281, 528, 847, 970, 1087
HapII CCGG 8 cut(s) 26, 37, 51, 80, 98, 282, 1113, 1129
HgaI GACGC 2 cut(s) 29, 223
HhaI GCGC 1 cut(s) 481
Hin1I GRCGYC 1 cut(s) 40
Hin1II CATG 8 cut(s) 124, 170, 257, 275, 485, 616, 637, 1184
Hin6I GCGC 1 cut(s) 479
HinP1I GCGC 1 cut(s) 479
HinfI GANTC 6 cut(s) 18, 368, 460, 499, 599, 718
HpaII CCGG 8 cut(s) 26, 37, 51, 80, 98, 282, 1113, 1129
HphI GGTGA 2 cut(s) 102, 436
Hpy188I TCNGA 2 cut(s) 298, 474
Hpy188III TCNNGA 4 cut(s) 464, 930, 990, 1155
Hpy99I CGWCG 1 cut(s) 217
HpyAV CCTTC 1 cut(s) 936
HpyCH4III ACNGT 1 cut(s) 1152
HpyCH4IV ACGT 1 cut(s) 802
HpyF10VI GCNNNNNNNGC 9 cut(s) 76, 259, 268, 394, 866, 887, 890, 896, 1007
HpyF3I CTNAG 6 cut(s) 227, 249, 276, 351, 530, 1173
HpySE526I ACGT 1 cut(s) 802
Hsp92I GRCGYC 1 cut(s) 40
Hsp92II CATG 8 cut(s) 124, 170, 257, 275, 485, 616, 637, 1184
HspAI GCGC 1 cut(s) 479
Kzo9I GATC 8 cut(s) 304, 466, 920, 982, 995, 1027, 1051, 1120
LmnI GCTCC 7 cut(s) 145, 235, 284, 318, 382, 1040, 1064
LweI GCATC 4 cut(s) 165, 946, 997, 1135
MaeI CTAG 1 cut(s) 639
MaeII ACGT 1 cut(s) 802
MaeIII GTNAC 1 cut(s) 628
MalI GATC 8 cut(s) 306, 468, 922, 984, 997, 1029, 1053, 1122
MboI GATC 8 cut(s) 304, 466, 920, 982, 995, 1027, 1051, 1120
MboII GAAGA 4 cut(s) 461, 631, 705, 788
MflI RGATCY 3 cut(s) 466, 920, 995
MhlI GDGCHC 2 cut(s) 86, 250
MluCI AATT 2 cut(s) 578, 676
MlyI GAGTC 2 cut(s) 377, 469
MmeI TCCRAC 2 cut(s) 276, 1148
MseI TTAA 3 cut(s) 375, 444, 1137
MslI CAYNNNNRTG 1 cut(s) 486
MspI CCGG 8 cut(s) 26, 37, 51, 80, 98, 282, 1113, 1129
MvaI CCWGG 8 cut(s) 72, 323, 608, 723, 797, 849, 939, 1089
MwoI GCNNNNNNNGC 9 cut(s) 76, 259, 268, 394, 866, 887, 890, 896, 1007
NciI CCSGG 5 cut(s) 51, 81, 99, 1129, 1130
NcoI CCATGG 1 cut(s) 633
NdeII GATC 8 cut(s) 304, 466, 920, 982, 995, 1027, 1051, 1120
NlaIII CATG 8 cut(s) 124, 170, 257, 275, 485, 616, 637, 1184
NlaIV GGNNCC 6 cut(s) 55, 85, 320, 1042, 1111, 1254
NspI RCATGY 4 cut(s) 124, 170, 275, 1184
PaeI GCATGC 1 cut(s) 275
PaeR7I CTCGAG 2 cut(s) 1230, 1263
PaqCI CACCTGC 1 cut(s) 382
PasI CCCWGGG 1 cut(s) 796
PciI ACATGT 2 cut(s) 120, 1180
PfeI GAWTC 4 cut(s) 18, 499, 599, 718
PflMI CCANNNNNTGG 2 cut(s) 205, 613
PfoI TCCNGGA 3 cut(s) 49, 721, 937
PinAI ACCGGT 1 cut(s) 1112
PleI GAGTC 2 cut(s) 376, 468
PpsI GAGTC 2 cut(s) 376, 468
PscI ACATGT 2 cut(s) 120, 1180
Psp6I CCWGG 8 cut(s) 70, 321, 606, 721, 795, 847, 937, 1087
PspGI CCWGG 8 cut(s) 70, 321, 606, 721, 795, 847, 937, 1087
PspN4I GGNNCC 6 cut(s) 55, 85, 320, 1042, 1111, 1254
PspPI GGNCC 8 cut(s) 28, 53, 68, 77, 845, 932, 968, 1169
PspXI VCTCGAGB 2 cut(s) 1230, 1263
PstI CTGCAG 3 cut(s) 264, 390, 760
PstNI CAGNNNCTG 5 cut(s) 154, 184, 205, 238, 268
PsuI RGATCY 3 cut(s) 466, 920, 995
RsaI GTAC 2 cut(s) 584, 1080
RsaNI GTAC 2 cut(s) 583, 1079
RseI CAYNNNNRTG 1 cut(s) 486
SaqAI TTAA 3 cut(s) 375, 444, 1137
Sau3AI GATC 8 cut(s) 304, 466, 920, 982, 995, 1027, 1051, 1120
Sau96I GGNCC 8 cut(s) 28, 53, 68, 77, 845, 932, 968, 1169
SchI GAGTC 2 cut(s) 377, 469
SduI GDGCHC 2 cut(s) 86, 250
SfaNI GCATC 4 cut(s) 165, 946, 997, 1135
SfcI CTRYAG 3 cut(s) 260, 386, 756
SfiI GGCCNNNNNGGCC 1 cut(s) 76
Sfr274I CTCGAG 2 cut(s) 1230, 1263
SinI GGWCC 3 cut(s) 53, 932, 1169
SlaI CTCGAG 2 cut(s) 1230, 1263
SmaI CCCGGG 1 cut(s) 1130
SmiMI CAYNNNNRTG 1 cut(s) 486
SmlI CTYRAG 2 cut(s) 1230, 1263
SmoI CTYRAG 2 cut(s) 1230, 1263
SphI GCATGC 1 cut(s) 275
Sse9I AATT 2 cut(s) 578, 676
SsiI CCGC 7 cut(s) 88, 326, 362, 365, 876, 891, 1256
SspMI CTAG 1 cut(s) 639
StyI CCWWGG 1 cut(s) 633
TaaI ACNGT 1 cut(s) 1152
TaiI ACGT 1 cut(s) 805
TaqI TCGA 5 cut(s) 16, 212, 522, 1231, 1264
TasI AATT 2 cut(s) 578, 676
TatI WGTACW 2 cut(s) 582, 1078
TauI GCSGC 2 cut(s) 365, 893
TfiI GAWTC 4 cut(s) 18, 499, 599, 718
Tru1I TTAA 3 cut(s) 375, 444, 1137
Tru9I TTAA 3 cut(s) 375, 444, 1137
TscAI CASTG 1 cut(s) 862
TspDTI ATGAA 4 cut(s) 305, 585, 747, 789
TspGWI ACGGA 1 cut(s) 900
TspMI CCCGGG 1 cut(s) 1128
TspRI CASTG 1 cut(s) 862
Van91I CCANNNNNTGG 2 cut(s) 205, 613
VpaK11BI GGWCC 3 cut(s) 53, 932, 1169
XagI CCTNNNNNAGG 3 cut(s) 230, 533, 1266
XceI RCATGY 4 cut(s) 124, 170, 275, 1184
XhoI CTCGAG 2 cut(s) 1230, 1263
XmaI CCCGGG 1 cut(s) 1128
XspI CTAG 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.