MD17G1072000.v1.1

FLX-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011129
Physical Location & Seq
Forward (+)
5781163 .. 5784229
3067 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1072000.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGGGAAGCAAAGGTCGAATTCCGCCGGCTCACATGCGTCGGCCGCTCTCCGGACCGGACCCATTTGGCCCGGGGATGCGGCCGCCCCATGGCGCCTATCCTCCGTTTGATATGTTGCCTCCTCCGCAAGTTATGGAGCAGAAGCTGGCTGCACAACATGTGGAGATGCAGAGGCTTGTGGTGGAGAACCAGAGGCTCGCCGCCACCCATGGAAGCCTGAGGCAAGAGCTGGCGGGTGCCCAACACGAGTTGCAGATATTACATGCCCAGATAGGAGCTATCAAGTCTGAGAGAGAACAGCAGATGAGTAGCCTTGTGGATAGTATAGCTAAGATGGAAGCTGACCTGAAGTCGGCTGAGCCTGTTAAGGCGGAGCTACAGAAGGCTCGAGCCGAGGCCCAGAGCTTGGTTGTTTCGAGGCAAGAACTCATCACCAATGTGCAACAACTCGGGCAGGATCTTCAGAGGACTCATGCAGATGTGCAGCAGATTCCGGCTTTGGTTGCACAGCTTGATGGACTGAGGCAGGAATATCAGCTATGCAGGGATACATATGACTATGAAAAGAGATTATACAGCAGCCACCTTGAATCACTTCAGGTCATGGAGAAGAACTATGTTACCATGGCTAGGGAGGTGGAAAAGCTCCGAGCAGAGTTACTGAATAATTCTAATGTTGATCGTAGAACTGGGGCCCCATATTATGCAACCCCGGGAAATAATGAGAACGAGGCTACTGGGCAGGCTATAGGACAAAATGCGTTTGAAGATAGCTACGGGGTTCAACAGGGACGTGCCCCCTTCCCTCCGGCTACAGCTGCGCCTGTTGCTGCTGGCGTTGGCGGTGCTGCTGCCACCGGGACTCCTCCATTAGTTGGAGCTCAATCTGGACCTCCTGGAAGGACTGGTTATGACGCACCAAGAGGGCCTGGCTATGATCCTTCAGGAGGACCTGCTCATGATGCACAGAGAGGACCTGCTTATGATTCACAGAGAGGGCCTGCTTATGATCCACAGAGAGGACCCGCTTATGACGCACAGAGACCTGGATATGATGTACAGAGACCGGGTTACGAAGTGCAAAGAGTGCCTGGTTATGATCCAACCCGGGTTGTCAACTATGATGCACAGTCTAGAGCTGCTGGTGGTGGTCCTCAAGGACATGTGCCCACAGGCAATGTGCATTATGGTTCTGCAACAACACCACCTGCTCGTGGTGGGAGTGGGTATGAGGCACCACCACCTCGAGGACCGGGAGGGGGAAACCCTGTTCGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

427

Amino Acids

45.7

Weight (kDa)

6.85

Isoelectric Point (pI)

51.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011129)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67170 AT1G67170
fragaria_vesca FvH4_6g45670 FvH4_6g45670 FvH4_6g45670
malus_domestica MD09G1080900.v1.1 MD17G1072000.v1.1
prunus_persica Prupe.3G242800_v2.0.a1
pyrus_communis pycom09g00730 pycom17g07130
rosa_chinensis RchiOBHm_Chr2g0163821
rosa_laevigata RLG00000021418
rosa_multiflora Rmu_sc0001181.1_g000004
rosa_roxburghii Rroxscaffold_2G00086990
rosa_rugosa Rorug02G0504800
rosa_samantha Rh2AG572700 Rh2BG583400 Rh2CG554300 Rh2DG593000
rosa_wichuraiana Rw2G047350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1216
AbsI CCTCGAGG 1 cut(s) 1245
Acc36I ACCTGC 3 cut(s) 961, 985, 1216
AccB1I GGYRCC 3 cut(s) 92, 236, 1234
AccB7I CCANNNNNTGG 2 cut(s) 406, 875
AccBSI CCGCTC 1 cut(s) 46
AccIII TCCGGA 1 cut(s) 50
AclWI GGATC 4 cut(s) 465, 932, 1004, 1094
AcoI YGGCCR 2 cut(s) 41, 80
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 4 cut(s) 368, 446, 581, 927
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 1 cut(s) 1059
AflIII ACRYGT 2 cut(s) 157, 1162
AgsI TTSAA 3 cut(s) 590, 767, 785
AhdI GACNNNNNGTC 1 cut(s) 349
AjiI CACGTC 1 cut(s) 794
AjnI CCWGG 4 cut(s) 895, 928, 1045, 1090
AleI CACNNNNGTG 1 cut(s) 437
AloI GAACNNNNNNTCC 1 cut(s) 1254
Alw21I GWGCWC 1 cut(s) 883
Alw26I GTCTC 2 cut(s) 1036, 1057
AlwI GGATC 4 cut(s) 465, 932, 1004, 1094
AlwNI CAGNNNCTG 1 cut(s) 145
Ama87I CYCGRG 6 cut(s) 70, 387, 449, 712, 1107, 1245
Aor13HI TCCGGA 1 cut(s) 50
AoxI GGCC 7 cut(s) 41, 67, 80, 396, 693, 926, 998
ApaI GGGCCC 1 cut(s) 697
ApeKI GCWGC 8 cut(s) 149, 484, 579, 818, 830, 848, 851, 1139
ApoI RAATTY 1 cut(s) 18
Asp700I GAANNNNTTC 1 cut(s) 594
AspLEI GCGC 2 cut(s) 95, 823
AsuC2I CCSGG 9 cut(s) 71, 72, 713, 714, 859, 1068, 1108, 1109, 1254
AsuHPI GGTGA 1 cut(s) 424
AsuII TTCGAA 1 cut(s) 1273
AvaI CYCGRG 6 cut(s) 70, 387, 449, 712, 1107, 1245
AvaII GGWCC 8 cut(s) 53, 58, 890, 950, 974, 1022, 1151, 1250
AxyI CCTNAGG 1 cut(s) 218
BaeGI GKGCMC 4 cut(s) 241, 697, 799, 1170
BanI GGYRCC 3 cut(s) 92, 236, 1234
BanII GRGCYC 2 cut(s) 697, 883
BauI CACGAG 2 cut(s) 245, 1212
Bbv12I GWGCWC 1 cut(s) 883
BbvI GCAGC 8 cut(s) 136, 496, 591, 805, 817, 835, 838, 1126
BccI CCATC 2 cut(s) 328, 509
BciT130I CCWGG 4 cut(s) 897, 930, 1047, 1092
BciVI GTATCC 1 cut(s) 541
BcnI CCSGG 9 cut(s) 71, 72, 713, 714, 859, 1068, 1108, 1109, 1254
BcoDI GTCTC 2 cut(s) 1036, 1057
BfaI CTAG 2 cut(s) 630, 1134
BfmI CTRYAG 3 cut(s) 377, 747, 813
BfoI RGCGCY 1 cut(s) 96
BfuAI ACCTGC 3 cut(s) 961, 985, 1216
BfuI GTATCC 1 cut(s) 541
BlpI GCTNAGC 1 cut(s) 357
Bme18I GGWCC 8 cut(s) 53, 58, 890, 950, 974, 1022, 1151, 1250
BmeRI GACNNNNNGTC 1 cut(s) 349
BmeT110I CYCGRG 6 cut(s) 70, 387, 449, 712, 1107, 1245
BmgBI CACGTC 1 cut(s) 794
BmiI GGNNCC 8 cut(s) 60, 94, 238, 694, 695, 696, 1024, 1236
BmrI ACTGGG 2 cut(s) 699, 747
BmsI GCATC 4 cut(s) 66, 156, 952, 1114
BmuI ACTGGG 2 cut(s) 699, 747
Bpu1102I GCTNAGC 1 cut(s) 357
Bpu14I TTCGAA 1 cut(s) 1273
BpuEI CTTGAG 1 cut(s) 1140
BpuMI CCSGG 9 cut(s) 71, 72, 713, 714, 859, 1068, 1108, 1109, 1254
BsaHI GRCGYC 1 cut(s) 93
BsaI GGTCTC 2 cut(s) 1036, 1057
BsaJI CCNNGG 9 cut(s) 70, 71, 88, 208, 393, 624, 711, 712, 1107
BsaWI WCCGGW 2 cut(s) 50, 55
BsaXI ACNNNNNCTCC 2 cut(s) 847, 877
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 3 cut(s) 694, 742, 910
Bse21I CCTNAGG 1 cut(s) 218
Bse3DI GCAATG 1 cut(s) 1183
BseAI TCCGGA 1 cut(s) 50
BseBI CCWGG 4 cut(s) 897, 930, 1047, 1092
BseDI CCNNGG 9 cut(s) 70, 71, 88, 208, 393, 624, 711, 712, 1107
BseGI GGATG 1 cut(s) 81
BseMI GCAATG 1 cut(s) 1183
BseMII CTCAG 4 cut(s) 209, 279, 348, 512
BseNI ACTGG 3 cut(s) 694, 742, 910
BseRI GAGGAG 2 cut(s) 111, 855
BseSI GKGCMC 4 cut(s) 241, 697, 799, 1170
BseX3I CGGCCG 2 cut(s) 41, 80
BseXI GCAGC 8 cut(s) 136, 496, 591, 805, 817, 835, 838, 1126
BsgI GTGCAG 2 cut(s) 135, 503
Bsh1285I CGRYCG 2 cut(s) 44, 83
BshFI GGCC 7 cut(s) 43, 69, 82, 398, 695, 928, 1000
BshNI GGYRCC 3 cut(s) 92, 236, 1234
BsiEI CGRYCG 2 cut(s) 44, 83
BsiHKAI GWGCWC 1 cut(s) 883
BsiHKCI CYCGRG 6 cut(s) 70, 387, 449, 712, 1107, 1245
BslFI GGGAC 2 cut(s) 804, 874
BsmAI GTCTC 2 cut(s) 1036, 1057
BsmFI GGGAC 2 cut(s) 804, 874
BsnI GGCC 7 cut(s) 43, 69, 82, 398, 695, 928, 1000
Bso31I GGTCTC 2 cut(s) 1036, 1057
BsoBI CYCGRG 6 cut(s) 70, 387, 449, 712, 1107, 1245
Bsp119I TTCGAA 1 cut(s) 1273
Bsp120I GGGCCC 1 cut(s) 693
Bsp1286I GDGCHC 5 cut(s) 241, 697, 799, 883, 1170
Bsp13I TCCGGA 1 cut(s) 50
Bsp1407I TGTACA 1 cut(s) 1057
Bsp143I GATC 5 cut(s) 457, 679, 937, 1009, 1099
Bsp1720I GCTNAGC 1 cut(s) 357
Bsp19I CCATGG 3 cut(s) 88, 208, 624
BspANI GGCC 7 cut(s) 43, 69, 82, 398, 695, 928, 1000
BspCNI CTCAG 4 cut(s) 210, 280, 349, 513
BspEI TCCGGA 1 cut(s) 50
BspHI TCATGA 1 cut(s) 958
BspLI GGNNCC 8 cut(s) 60, 94, 238, 694, 695, 696, 1024, 1236
BspMI ACCTGC 3 cut(s) 961, 985, 1216
BspPI GGATC 4 cut(s) 465, 932, 1004, 1094
BspT104I TTCGAA 1 cut(s) 1273
BspT107I GGYRCC 3 cut(s) 92, 236, 1234
BspTNI GGTCTC 2 cut(s) 1036, 1057
BsrBI CCGCTC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 1183
BsrFI RCCGGY 1 cut(s) 25
BsrGI TGTACA 1 cut(s) 1057
BsrI ACTGG 3 cut(s) 694, 742, 910
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 9 cut(s) 70, 71, 88, 208, 393, 624, 711, 712, 1107
BssMI GATC 5 cut(s) 457, 679, 937, 1009, 1099
BssNI GRCGYC 1 cut(s) 93
BssSI CACGAG 2 cut(s) 245, 1212
BssT1I CCWWGG 3 cut(s) 88, 208, 624
Bst2BI CACGAG 2 cut(s) 245, 1212
Bst2UI CCWGG 4 cut(s) 897, 930, 1047, 1092
Bst4CI ACNGT 1 cut(s) 1131
BstACI GRCGYC 1 cut(s) 93
BstAPI GCANNNNNTGC 1 cut(s) 1087
BstAUI TGTACA 1 cut(s) 1057
BstBI TTCGAA 1 cut(s) 1273
BstC8I GCNNGC 7 cut(s) 27, 147, 198, 231, 744, 835, 1002
BstDEI CTNAG 5 cut(s) 218, 288, 330, 357, 521
BstDSI CCRYGG 3 cut(s) 88, 208, 624
BstENI CCTNNNNNAGG 1 cut(s) 945
BstF5I GGATG 1 cut(s) 81
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 2 cut(s) 95, 823
BstKTI GATC 5 cut(s) 460, 682, 940, 1012, 1102
BstMAI GTCTC 2 cut(s) 1036, 1057
BstMBI GATC 5 cut(s) 457, 679, 937, 1009, 1099
BstMCI CGRYCG 2 cut(s) 44, 83
BstMWI GCNNNNNNNGC 8 cut(s) 43, 124, 503, 818, 827, 962, 1034, 1087
BstNI CCWGG 4 cut(s) 897, 930, 1047, 1092
BstNSI RCATGY 4 cut(s) 37, 161, 266, 1166
BstSFI CTRYAG 3 cut(s) 377, 747, 813
BstSLI GKGCMC 4 cut(s) 241, 697, 799, 1170
BstV1I GCAGC 8 cut(s) 136, 496, 591, 805, 817, 835, 838, 1126
BstX2I RGATCY 1 cut(s) 457
BstYI RGATCY 1 cut(s) 457
BstZI CGGCCG 2 cut(s) 41, 80
Bsu36I CCTNAGG 1 cut(s) 218
BsuI GTATCC 1 cut(s) 541
BsuRI GGCC 7 cut(s) 43, 69, 82, 398, 695, 928, 1000
BtgI CCRYGG 3 cut(s) 88, 208, 624
BtrI CACGTC 1 cut(s) 794
BtsCI GGATG 1 cut(s) 81
BveI ACCTGC 3 cut(s) 961, 985, 1216
Cac8I GCNNGC 7 cut(s) 27, 147, 198, 231, 744, 835, 1002
CaiI CAGNNNCTG 1 cut(s) 145
CciI TCATGA 1 cut(s) 958
CciNI GCGGCCGC 1 cut(s) 80
CfoI GCGC 2 cut(s) 95, 823
Cfr10I RCCGGY 1 cut(s) 25
Cfr9I CCCGGG 3 cut(s) 70, 712, 1107
CpoI CGGWCCG 1 cut(s) 53
CseI GACGC 3 cut(s) 26, 923, 1043
Csp6I GTAC 1 cut(s) 1058
CspI CGGWCCG 1 cut(s) 53
CviQI GTAC 1 cut(s) 1058
DdeI CTNAG 5 cut(s) 218, 288, 330, 357, 521
DinI GGCGCC 1 cut(s) 94
DpnI GATC 5 cut(s) 459, 681, 939, 1011, 1101
DpnII GATC 5 cut(s) 457, 679, 937, 1009, 1099
DriI GACNNNNNGTC 1 cut(s) 349
EaeI YGGCCR 2 cut(s) 41, 80
EagI CGGCCG 2 cut(s) 41, 80
Eam1105I GACNNNNNGTC 1 cut(s) 349
EciI GGCGGA 2 cut(s) 12, 386
Ecl136II GAGCTC 1 cut(s) 881
EclXI CGGCCG 2 cut(s) 41, 80
Eco130I CCWWGG 3 cut(s) 88, 208, 624
Eco24I GRGCYC 2 cut(s) 697, 883
Eco31I GGTCTC 2 cut(s) 1036, 1057
Eco47I GGWCC 8 cut(s) 53, 58, 890, 950, 974, 1022, 1151, 1250
Eco52I CGGCCG 2 cut(s) 41, 80
Eco53kI GAGCTC 1 cut(s) 881
Eco57I CTGAAG 4 cut(s) 368, 446, 581, 927
Eco81I CCTNAGG 1 cut(s) 218
Eco88I CYCGRG 6 cut(s) 70, 387, 449, 712, 1107, 1245
EcoICRI GAGCTC 1 cut(s) 881
EcoNI CCTNNNNNAGG 1 cut(s) 945
EcoO109I RGGNCCY 7 cut(s) 693, 694, 926, 950, 974, 998, 1022
EcoRI GAATTC 1 cut(s) 18
EcoRII CCWGG 4 cut(s) 895, 928, 1045, 1090
EcoT14I CCWWGG 3 cut(s) 88, 208, 624
EcoT38I GRGCYC 2 cut(s) 697, 883
EgeI GGCGCC 1 cut(s) 94
EheI GGCGCC 1 cut(s) 94
ErhI CCWWGG 3 cut(s) 88, 208, 624
FaqI GGGAC 2 cut(s) 804, 874
FauI CCCGC 2 cut(s) 226, 1033
FauNDI CATATG 1 cut(s) 553
FokI GGATG 1 cut(s) 88
FriOI GRGCYC 2 cut(s) 697, 883
FspBI CTAG 2 cut(s) 630, 1134
GlaI GCGC 2 cut(s) 94, 822
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 7 cut(s) 43, 69, 82, 398, 695, 928, 1000
HgaI GACGC 3 cut(s) 26, 923, 1043
HhaI GCGC 2 cut(s) 95, 823
Hin1I GRCGYC 1 cut(s) 93
Hin6I GCGC 2 cut(s) 93, 821
HinP1I GCGC 2 cut(s) 93, 821
HincII GTYRAC 1 cut(s) 1117
HindII GTYRAC 1 cut(s) 1117
HinfI GANTC 5 cut(s) 469, 490, 590, 862, 986
HphI GGTGA 1 cut(s) 424
Hpy166II GTNNAC 1 cut(s) 1117
Hpy188I TCNGA 3 cut(s) 289, 465, 650
Hpy188III TCNNGA 5 cut(s) 51, 888, 945, 959, 1134
Hpy8I GTNNAC 1 cut(s) 1117
Hpy99I CGWCG 1 cut(s) 42
HpyAV CCTTC 4 cut(s) 376, 811, 894, 951
HpyCH4III ACNGT 1 cut(s) 1131
HpyCH4IV ACGT 1 cut(s) 793
HpyF10VI GCNNNNNNNGC 8 cut(s) 43, 124, 503, 818, 827, 962, 1034, 1087
HpyF3I CTNAG 5 cut(s) 218, 288, 330, 357, 521
HpySE526I ACGT 1 cut(s) 793
Hsp92I GRCGYC 1 cut(s) 93
HspAI GCGC 2 cut(s) 93, 821
KasI GGCGCC 1 cut(s) 92
Kpn2I TCCGGA 1 cut(s) 50
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Kzo9I GATC 5 cut(s) 457, 679, 937, 1009, 1099
LmnI GCTCC 5 cut(s) 136, 275, 373, 651, 878
Lsp1109I GCAGC 8 cut(s) 136, 496, 591, 805, 817, 835, 838, 1126
LweI GCATC 4 cut(s) 66, 156, 952, 1114
MaeI CTAG 2 cut(s) 630, 1134
MaeII ACGT 1 cut(s) 793
MaeIII GTNAC 3 cut(s) 619, 657, 1070
MalI GATC 5 cut(s) 459, 681, 939, 1011, 1101
MbiI CCGCTC 1 cut(s) 46
MboI GATC 5 cut(s) 457, 679, 937, 1009, 1099
MboII GAAGA 3 cut(s) 452, 622, 779
MflI RGATCY 1 cut(s) 457
MhlI GDGCHC 5 cut(s) 241, 697, 799, 883, 1170
MluCI AATT 2 cut(s) 18, 667
Mly113I GGCGCC 1 cut(s) 93
MlyI GAGTC 2 cut(s) 463, 856
MmeI TCCRAC 2 cut(s) 856, 1127
MroI TCCGGA 1 cut(s) 50
MroNI GCCGGC 1 cut(s) 25
MroXI GAANNNNTTC 1 cut(s) 594
MseI TTAA 1 cut(s) 366
MslI CAYNNNNRTG 2 cut(s) 437, 477
MspA1I CMGCKG 1 cut(s) 818
MvaI CCWGG 4 cut(s) 897, 930, 1047, 1092
MwoI GCNNNNNNNGC 8 cut(s) 43, 124, 503, 818, 827, 962, 1034, 1087
NaeI GCCGGC 1 cut(s) 27
NarI GGCGCC 1 cut(s) 93
NciI CCSGG 9 cut(s) 71, 72, 713, 714, 859, 1068, 1108, 1109, 1254
NcoI CCATGG 3 cut(s) 88, 208, 624
NdeI CATATG 1 cut(s) 553
NdeII GATC 5 cut(s) 457, 679, 937, 1009, 1099
NgoMIV GCCGGC 1 cut(s) 25
NlaIV GGNNCC 8 cut(s) 60, 94, 238, 694, 695, 696, 1024, 1236
NmeAIII GCCGAG 1 cut(s) 418
NotI GCGGCCGC 1 cut(s) 80
NspI RCATGY 4 cut(s) 37, 161, 266, 1166
NspV TTCGAA 1 cut(s) 1273
OliI CACNNNNGTG 1 cut(s) 437
PaeR7I CTCGAG 2 cut(s) 387, 1245
PagI TCATGA 1 cut(s) 958
PaqCI CACCTGC 1 cut(s) 1216
PciI ACATGT 2 cut(s) 157, 1162
PdiI GCCGGC 1 cut(s) 27
PdmI GAANNNNTTC 1 cut(s) 594
PfeI GAWTC 3 cut(s) 490, 590, 986
PflMI CCANNNNNTGG 2 cut(s) 406, 875
PfoI TCCNGGA 1 cut(s) 895
PleI GAGTC 2 cut(s) 463, 856
PluTI GGCGCC 1 cut(s) 96
PpsI GAGTC 2 cut(s) 463, 856
PpuMI RGGWCCY 3 cut(s) 950, 974, 1022
PscI ACATGT 2 cut(s) 157, 1162
Psp124BI GAGCTC 1 cut(s) 883
Psp5II RGGWCCY 3 cut(s) 950, 974, 1022
Psp6I CCWGG 4 cut(s) 895, 928, 1045, 1090
PspGI CCWGG 4 cut(s) 895, 928, 1045, 1090
PspN4I GGNNCC 8 cut(s) 60, 94, 238, 694, 695, 696, 1024, 1236
PspOMI GGGCCC 1 cut(s) 693
PspPPI RGGWCCY 3 cut(s) 950, 974, 1022
PspXI VCTCGAGB 2 cut(s) 387, 1245
PstNI CAGNNNCTG 1 cut(s) 145
PsuI RGATCY 1 cut(s) 457
PvuII CAGCTG 1 cut(s) 818
RsaI GTAC 1 cut(s) 1059
RsaNI GTAC 1 cut(s) 1058
RseI CAYNNNNRTG 2 cut(s) 437, 477
Rsr2I CGGWCCG 1 cut(s) 53
RsrII CGGWCCG 1 cut(s) 53
SacI GAGCTC 1 cut(s) 883
SaqAI TTAA 1 cut(s) 366
Sau3AI GATC 5 cut(s) 457, 679, 937, 1009, 1099
SchI GAGTC 2 cut(s) 463, 856
SduI GDGCHC 5 cut(s) 241, 697, 799, 883, 1170
SfaNI GCATC 4 cut(s) 66, 156, 952, 1114
SfcI CTRYAG 3 cut(s) 377, 747, 813
SfoI GGCGCC 1 cut(s) 94
Sfr274I CTCGAG 2 cut(s) 387, 1245
SfuI TTCGAA 1 cut(s) 1273
SinI GGWCC 8 cut(s) 53, 58, 890, 950, 974, 1022, 1151, 1250
SlaI CTCGAG 2 cut(s) 387, 1245
SmaI CCCGGG 3 cut(s) 72, 714, 1109
SmiMI CAYNNNNRTG 2 cut(s) 437, 477
SmlI CTYRAG 3 cut(s) 387, 1155, 1245
SmoI CTYRAG 3 cut(s) 387, 1155, 1245
Sse9I AATT 2 cut(s) 18, 667
SspDI GGCGCC 1 cut(s) 92
SspMI CTAG 2 cut(s) 630, 1134
SstI GAGCTC 1 cut(s) 883
StyI CCWWGG 3 cut(s) 88, 208, 624
TaaI ACNGT 1 cut(s) 1131
TaiI ACGT 1 cut(s) 796
TaqI TCGA 5 cut(s) 16, 388, 416, 1246, 1273
TasI AATT 2 cut(s) 18, 667
TatI WGTACW 1 cut(s) 1057
TauI GCSGC 4 cut(s) 46, 82, 85, 203
TfiI GAWTC 3 cut(s) 490, 590, 986
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TseI GCWGC 8 cut(s) 149, 484, 579, 818, 830, 848, 851, 1139
TspDTI ATGAA 1 cut(s) 576
TspGWI ACGGA 1 cut(s) 93
TspMI CCCGGG 3 cut(s) 70, 712, 1107
Van91I CCANNNNNTGG 2 cut(s) 406, 875
VpaK11BI GGWCC 8 cut(s) 53, 58, 890, 950, 974, 1022, 1151, 1250
XagI CCTNNNNNAGG 1 cut(s) 945
XapI RAATTY 1 cut(s) 18
XbaI TCTAGA 1 cut(s) 1133
XceI RCATGY 4 cut(s) 37, 161, 266, 1166
XhoI CTCGAG 2 cut(s) 387, 1245
XmaI CCCGGG 3 cut(s) 70, 712, 1107
XmnI GAANNNNTTC 1 cut(s) 594
XspI CTAG 2 cut(s) 630, 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.