Rh2CG554400

tryptophan synthase beta chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0016013
Physical Location & Seq
Reverse (-)
73296063 .. 73299220
3158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG554400.1

Sequence Viewer

Length: 1284 bp
ATGGCTACTCACTGTGTTAATTTATGTGCAAGTTCATTTGATACTAAGCCTTCTTCATCAGAATCACACTCCAGAGTTGAGAAGAAATGGCTTGGATCTATTGCACTTGCTAGAAAGCCGAAGAATTTGAAGCTTTCAAATGGCTGTAGAGCAAGAGCAGCCCTCAATGCTGATTCAATACCAATCGAAATCCCTCGTCAGTGGTACAACTTGATTGCAGATCTTCCTATAAAACCTCCTCCTTCATTGCATCCCAAGACTTTTGAACCAGTAAAACCTGAAGATCTGTCCCCTCTATTTCCTGATGAGTTGATTAAACAGGAAGCAAGTGGTGAAAGATACATAGATATCCCAGAAGAAGTTCGAGAGATATACAAGCTTTGGCGTCCAACCCCTCTCATTAGGGCAAAGAGGTTGGAGAAGCTTCTTGACACGCCTGCCAGAATTTATTACAAGTATGAAGGTGTTAGCCCTGCTGGATCACACAAACCCAACACTGCAGTTCCTCAAGTGTATTATAATGCACAACAAGGCATCAAGAAGGTTGTGACAGAAACAGGAGCTGGTCAATGGGGATGTTCTTTGGCTTTTGCATCCAGCTTATTCGGTCTTGAATGTGAGGTATGGCAAGTGCGTGCTTCGTATGATCAGAAACCATATCGCAAATTGATGATGCAAACTTGGGGGGCAAAGGTACACCCGTCTCCATCGAACATAACTGAATCAGGTAGAAAAATCCTTCTGATGGATCCATCGACCCCGGGAAGTTTAGGAATAGCTATTTCTGAAGCTGTGGAGGTTGCAGCTATGAATGCTGACACTAAATACTGTCTGGGAAGTGTTCTCAATCATGTTTTGCTCCACCAGACTGTAATAGGTGAAGAGTGTCTGAAACAAATGGAAGCGATAGGTGAAACCCCGGACTTGATCATAGGGTGTACTGGTGGAGGATCCAACTTTGCAGGGCTAAGTTTCCCATTCATTAGAGAAAAGCTCGATGGGAAAATGAGCCCTGTAATTAGAGCAGTTGAGCCTTCTGCTTGTCCTTCATTAACTAAAGGTGTATACAGATACGATTATGGTGACACAGCAGGGATGACTCCACTAATGAAGATGCATACACTTGGGCATGACTTCATTCCGGACCCAATTCATGCCGGAGGATTGCGTTATCATGGAATGGCTCCATTGATTTCTCACGTTTATGAACTCGGTTTCATGGAAGCAATTTCAATTCCCCAGACTGAGTGCTTCAAAGGTATTTCAAATTTTCTAAATACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00400 ko01100 ko01110 ko01130 ko01230 map00260 map00400 map01100 map01110 map01130 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

427

Amino Acids

47.07

Weight (kDa)

7.96

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 127 - 421 4.8e-32 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016013)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38530
fragaria_vesca FvH4_6g45680
malus_domestica MD17G1071900.v1.1
prunus_persica Prupe.3G243000_v2.0.a1
pyrus_communis pycom17g07120
rosa_chinensis RchiOBHm_Chr2g0163831
rosa_laevigata RLG00000021419
rosa_multiflora Rmu_sc0005917.1_g000003
rosa_roxburghii Rroxscaffold_2G00086980
rosa_rugosa Rorug02G0504800
rosa_samantha Rh2AG572800 Rh2BG583500 Rh2CG554400 Rh2DG593100
rosa_wichuraiana Rw2G047360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 519
AccI GTMKAC 1 cut(s) 1065
AccIII TCCGGA 1 cut(s) 1141
AclWI GGATC 6 cut(s) 103, 487, 743, 756, 945, 958
AcsI RAATTY 3 cut(s) 124, 444, 1267
AcuI CTGAAG 2 cut(s) 300, 807
AcyI GRCGYC 1 cut(s) 385
AfaI GTAC 3 cut(s) 206, 696, 940
AfiI CCNNNNNNNGG 1 cut(s) 745
AgsI TTSAA 8 cut(s) 130, 138, 177, 266, 614, 1233, 1255, 1266
AluBI AGCT 9 cut(s) 133, 379, 424, 563, 600, 779, 791, 806, 994
AluI AGCT 9 cut(s) 133, 379, 424, 563, 600, 779, 791, 806, 994
Alw26I GTCTC 1 cut(s) 708
AlwI GGATC 6 cut(s) 103, 487, 743, 756, 945, 958
AlwNI CAGNNNCTG 1 cut(s) 563
Ama87I CYCGRG 1 cut(s) 760
Aor13HI TCCGGA 1 cut(s) 1141
ApeKI GCWGC 2 cut(s) 158, 803
ApoI RAATTY 3 cut(s) 124, 444, 1267
Asp700I GAANNNNTTC 1 cut(s) 360
AspS9I GGNCC 1 cut(s) 1144
AsuC2I CCSGG 3 cut(s) 761, 762, 920
AsuHPI GGTGA 4 cut(s) 344, 890, 923, 1094
AvaI CYCGRG 1 cut(s) 760
AvaII GGWCC 1 cut(s) 1144
BaeI ACNNNNGTAYC 2 cut(s) 686, 719
BamHI GGATCC 2 cut(s) 748, 950
BanII GRGCYC 1 cut(s) 1013
BbvI GCAGC 2 cut(s) 170, 815
BccI CCATC 4 cut(s) 715, 739, 760, 992
BclI TGATCA 2 cut(s) 646, 927
BcnI CCSGG 3 cut(s) 761, 762, 920
BcoDI GTCTC 1 cut(s) 708
BfaI CTAG 1 cut(s) 111
BfmI CTRYAG 2 cut(s) 145, 498
BglII AGATCT 2 cut(s) 220, 283
BisI GCNGC 2 cut(s) 159, 804
BlsI GCNGC 2 cut(s) 160, 805
Bme1390I CCNGG 3 cut(s) 761, 762, 920
Bme18I GGWCC 1 cut(s) 1144
BmeT110I CYCGRG 1 cut(s) 760
BmgT120I GGNCC 1 cut(s) 1144
BmiI GGNNCC 4 cut(s) 750, 952, 1146, 1185
BmrFI CCNGG 3 cut(s) 761, 762, 920
BmsI GCATC 5 cut(s) 259, 543, 602, 663, 1104
BplI GAGNNNNNCTC 4 cut(s) 147, 179, 978, 1010
BpmI CTGGAG 1 cut(s) 55
BpuEI CTTGAG 1 cut(s) 492
BpuMI CCSGG 3 cut(s) 761, 762, 920
BsaHI GRCGYC 1 cut(s) 385
BsaJI CCNNGG 3 cut(s) 759, 760, 918
BsaWI WCCGGW 1 cut(s) 1141
Bsc4I CCNNNNNNNGG 1 cut(s) 745
Bse1I ACTGG 2 cut(s) 269, 946
Bse3DI GCAATG 1 cut(s) 245
BseAI TCCGGA 1 cut(s) 1141
BseDI CCNNGG 3 cut(s) 759, 760, 918
BseGI GGATG 4 cut(s) 250, 581, 593, 1101
BseLI CCNNNNNNNGG 1 cut(s) 745
BseMI GCAATG 1 cut(s) 245
BseMII CTCAG 1 cut(s) 1236
BseNI ACTGG 2 cut(s) 269, 946
BseRI GAGGAG 1 cut(s) 228
BseXI GCAGC 2 cut(s) 170, 815
BsiHKCI CYCGRG 1 cut(s) 760
BsiSI CCGG 4 cut(s) 761, 920, 1142, 1158
BslFI GGGAC 1 cut(s) 274
BslI CCNNNNNNNGG 1 cut(s) 745
BsmAI GTCTC 1 cut(s) 708
BsmBI CGTCTC 1 cut(s) 708
BsmFI GGGAC 1 cut(s) 274
BsmI GAATGC 1 cut(s) 817
BsoBI CYCGRG 1 cut(s) 760
Bsp1286I GDGCHC 1 cut(s) 1013
Bsp13I TCCGGA 1 cut(s) 1141
Bsp143I GATC 8 cut(s) 95, 220, 283, 479, 646, 748, 927, 950
BspCNI CTCAG 1 cut(s) 1237
BspEI TCCGGA 1 cut(s) 1141
BspLI GGNNCC 4 cut(s) 750, 952, 1146, 1185
BspMAI CTGCAG 1 cut(s) 502
BspPI GGATC 6 cut(s) 103, 487, 743, 756, 945, 958
BsrDI GCAATG 1 cut(s) 245
BsrI ACTGG 2 cut(s) 269, 946
BssECI CCNNGG 3 cut(s) 759, 760, 918
BssMI GATC 8 cut(s) 95, 220, 283, 479, 646, 748, 927, 950
BssNAI GTATAC 1 cut(s) 1066
BssNI GRCGYC 1 cut(s) 385
Bst1107I GTATAC 1 cut(s) 1066
Bst4CI ACNGT 3 cut(s) 14, 830, 871
Bst6I CTCTTC 1 cut(s) 876
BstACI GRCGYC 1 cut(s) 385
BstC8I GCNNGC 2 cut(s) 438, 636
BstDEI CTNAG 3 cut(s) 45, 968, 1245
BstF5I GGATG 4 cut(s) 250, 581, 593, 1101
BstKTI GATC 8 cut(s) 98, 223, 286, 482, 649, 751, 930, 953
BstMAI GTCTC 1 cut(s) 708
BstMBI GATC 8 cut(s) 95, 220, 283, 479, 646, 748, 927, 950
BstMWI GCNNNNNNNGC 3 cut(s) 158, 167, 812
BstSCI CCNGG 3 cut(s) 759, 760, 918
BstSFI CTRYAG 2 cut(s) 145, 498
BstV1I GCAGC 2 cut(s) 170, 815
BstX2I RGATCY 5 cut(s) 95, 220, 283, 748, 950
BstYI RGATCY 5 cut(s) 95, 220, 283, 748, 950
BstZ17I GTATAC 1 cut(s) 1066
BtsCI GGATG 4 cut(s) 250, 581, 593, 1101
BtsI GCAGTG 1 cut(s) 495
BtsIMutI CAGTG 3 cut(s) 10, 206, 495
Cac8I GCNNGC 2 cut(s) 438, 636
CaiI CAGNNNCTG 1 cut(s) 563
Cfr13I GGNCC 1 cut(s) 1144
Cfr9I CCCGGG 1 cut(s) 760
CseI GACGC 1 cut(s) 374
Csp6I GTAC 3 cut(s) 205, 695, 939
CviAII CATG 5 cut(s) 851, 1130, 1154, 1175, 1219
CviQI GTAC 3 cut(s) 205, 695, 939
DdeI CTNAG 3 cut(s) 45, 968, 1245
DpnI GATC 8 cut(s) 97, 222, 285, 481, 648, 750, 929, 952
DpnII GATC 8 cut(s) 95, 220, 283, 479, 646, 748, 927, 950
Eam1104I CTCTTC 1 cut(s) 876
EarI CTCTTC 1 cut(s) 876
Eco24I GRGCYC 1 cut(s) 1013
Eco32I GATATC 1 cut(s) 349
Eco47I GGWCC 1 cut(s) 1144
Eco57I CTGAAG 2 cut(s) 300, 807
Eco88I CYCGRG 1 cut(s) 760
EcoRV GATATC 1 cut(s) 349
EcoT22I ATGCAT 1 cut(s) 1119
EcoT38I GRGCYC 1 cut(s) 1013
Esp3I CGTCTC 1 cut(s) 708
FaeI CATG 5 cut(s) 854, 1133, 1157, 1178, 1222
FaqI GGGAC 1 cut(s) 274
FatI CATG 5 cut(s) 850, 1129, 1153, 1174, 1218
FbaI TGATCA 2 cut(s) 646, 927
FblI GTMKAC 1 cut(s) 1065
Fnu4HI GCNGC 2 cut(s) 159, 804
FokI GGATG 4 cut(s) 237, 580, 588, 1108
FriOI GRGCYC 1 cut(s) 1013
Fsp4HI GCNGC 2 cut(s) 159, 804
FspBI CTAG 1 cut(s) 111
GluI GCNGC 2 cut(s) 159, 804
GsuI CTGGAG 1 cut(s) 55
HapII CCGG 4 cut(s) 761, 920, 1142, 1158
HgaI GACGC 1 cut(s) 374
Hin1I GRCGYC 1 cut(s) 385
Hin1II CATG 5 cut(s) 854, 1133, 1157, 1178, 1222
HindIII AAGCTT 3 cut(s) 131, 377, 422
HinfI GANTC 4 cut(s) 62, 173, 722, 1099
HpaII CCGG 4 cut(s) 761, 920, 1142, 1158
HphI GGTGA 4 cut(s) 344, 890, 923, 1094
Hpy166II GTNNAC 3 cut(s) 697, 939, 1066
Hpy188I TCNGA 5 cut(s) 61, 651, 744, 787, 891
Hpy188III TCNNGA 7 cut(s) 72, 302, 365, 428, 538, 611, 1142
Hpy8I GTNNAC 3 cut(s) 697, 939, 1066
HpyAV CCTTC 7 cut(s) 60, 252, 455, 535, 749, 1044, 1056
HpyCH4III ACNGT 3 cut(s) 14, 830, 871
HpyCH4IV ACGT 1 cut(s) 1200
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 167, 812
HpyF3I CTNAG 3 cut(s) 45, 968, 1245
HpySE526I ACGT 1 cut(s) 1200
Hsp92I GRCGYC 1 cut(s) 385
Hsp92II CATG 5 cut(s) 854, 1133, 1157, 1178, 1222
Kpn2I TCCGGA 1 cut(s) 1141
Ksp22I TGATCA 2 cut(s) 646, 927
Kzo9I GATC 8 cut(s) 95, 220, 283, 479, 646, 748, 927, 950
LmnI GCTCC 3 cut(s) 560, 864, 1189
Lsp1109I GCAGC 2 cut(s) 170, 815
LweI GCATC 5 cut(s) 259, 543, 602, 663, 1104
MaeI CTAG 1 cut(s) 111
MaeII ACGT 1 cut(s) 1200
MaeIII GTNAC 2 cut(s) 547, 1082
MalI GATC 8 cut(s) 97, 222, 285, 481, 648, 750, 929, 952
MboI GATC 8 cut(s) 95, 220, 283, 479, 646, 748, 927, 950
MboII GAAGA 8 cut(s) 45, 94, 133, 215, 293, 368, 893, 1123
MflI RGATCY 5 cut(s) 95, 220, 283, 748, 950
MhlI GDGCHC 1 cut(s) 1013
MluCI AATT 9 cut(s) 19, 124, 444, 665, 1017, 1149, 1227, 1233, 1267
MlyI GAGTC 1 cut(s) 1093
MmeI TCCRAC 3 cut(s) 396, 413, 978
Mph1103I ATGCAT 1 cut(s) 1119
MroI TCCGGA 1 cut(s) 1141
MroXI GAANNNNTTC 1 cut(s) 360
MseI TTAA 3 cut(s) 18, 315, 1052
MslI CAYNNNNRTG 1 cut(s) 1203
MspI CCGG 4 cut(s) 761, 920, 1142, 1158
MspR9I CCNGG 3 cut(s) 761, 762, 920
Mva1269I GAATGC 1 cut(s) 817
MwoI GCNNNNNNNGC 3 cut(s) 158, 167, 812
NciI CCSGG 3 cut(s) 761, 762, 920
NdeII GATC 8 cut(s) 95, 220, 283, 479, 646, 748, 927, 950
NlaIII CATG 5 cut(s) 854, 1133, 1157, 1178, 1222
NlaIV GGNNCC 4 cut(s) 750, 952, 1146, 1185
NmuCI GTSAC 2 cut(s) 547, 1082
NsiI ATGCAT 1 cut(s) 1119
PctI GAATGC 1 cut(s) 817
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 3 cut(s) 62, 173, 722
PkrI GCNGC 2 cut(s) 160, 805
PleI GAGTC 1 cut(s) 1093
PpsI GAGTC 1 cut(s) 1093
PsiI TTATAA 1 cut(s) 519
PspN4I GGNNCC 4 cut(s) 750, 952, 1146, 1185
PspPI GGNCC 1 cut(s) 1144
PstI CTGCAG 1 cut(s) 502
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 5 cut(s) 95, 220, 283, 748, 950
RsaI GTAC 3 cut(s) 206, 696, 940
RsaNI GTAC 3 cut(s) 205, 695, 939
RseI CAYNNNNRTG 1 cut(s) 1203
SaqAI TTAA 3 cut(s) 18, 315, 1052
SatI GCNGC 2 cut(s) 159, 804
Sau3AI GATC 8 cut(s) 95, 220, 283, 479, 646, 748, 927, 950
Sau96I GGNCC 1 cut(s) 1144
SchI GAGTC 1 cut(s) 1093
ScrFI CCNGG 3 cut(s) 761, 762, 920
SduI GDGCHC 1 cut(s) 1013
SfaNI GCATC 5 cut(s) 259, 543, 602, 663, 1104
SfcI CTRYAG 2 cut(s) 145, 498
SinI GGWCC 1 cut(s) 1144
SmaI CCCGGG 1 cut(s) 762
SmiMI CAYNNNNRTG 1 cut(s) 1203
SmlI CTYRAG 1 cut(s) 507
SmoI CTYRAG 1 cut(s) 507
Sse9I AATT 9 cut(s) 19, 124, 444, 665, 1017, 1149, 1227, 1233, 1267
SspMI CTAG 1 cut(s) 111
StyD4I CCNGG 3 cut(s) 759, 760, 918
TaaI ACNGT 3 cut(s) 14, 830, 871
TaiI ACGT 1 cut(s) 1203
TaqI TCGA 5 cut(s) 186, 364, 710, 755, 996
TaqII GACCGA 1 cut(s) 596
TasI AATT 9 cut(s) 19, 124, 444, 665, 1017, 1149, 1227, 1233, 1267
TatI WGTACW 1 cut(s) 938
TfiI GAWTC 3 cut(s) 62, 173, 722
Tru1I TTAA 3 cut(s) 18, 315, 1052
Tru9I TTAA 3 cut(s) 18, 315, 1052
TscAI CASTG 3 cut(s) 17, 206, 502
TseFI GTSAC 2 cut(s) 547, 1082
TseI GCWGC 2 cut(s) 158, 803
Tsp45I GTSAC 2 cut(s) 547, 1082
TspMI CCCGGG 1 cut(s) 760
TspRI CASTG 3 cut(s) 17, 206, 502
VpaK11BI GGWCC 1 cut(s) 1144
XapI RAATTY 3 cut(s) 124, 444, 1267
XmaI CCCGGG 1 cut(s) 760
XmiI GTMKAC 1 cut(s) 1065
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 1 cut(s) 111
Zsp2I ATGCAT 1 cut(s) 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.