FvH4_6g49660

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011589
Physical Location & Seq
Reverse (-)
37158219 .. 37161537
3319 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g49660.t2

Sequence Viewer

Length: 1323 bp
ATGGGAGCATGTGTGTCGAGGCCGAAGGGCTGGGCGATGGGATTGTCAAAGAAGAAGAAGAACGGCGGCCACCGGCAAAAACGGATCATCCGGCGGCGTGTTTCCTCCTCCAACAAATCCACCAACCCTCAACACCAAGGGAGTATGGATAGCGCGTGGTTTGATTGTACTTCAGTGATGGATTCGGAGCGAGGAGATGAAGAGTTCTACAGTGTGTATGATGATGTGGTGTCTTTAAACGGTTCGGAGAGCGCGTTACAGAGGACTAGGGAGGGTGGTGGGGATGAGAGTGACAGAATGCAAGGGTTGGCGGTTGTTCAGAATAACTGCCTGCCTTGTATTCCTTCGAGTAGTCCTCCAGCGGTGGAGAACAAGTTGAGTTTTCGGAAGAGGTTGGGGCCGTCTAGGCTTTCGTTTAAGTGGAGGGAAGGGCATGCTGCTGCTGCTGCTGCTGCTCCAGCTGATCCCACATTAGTTTCGCCCAGGGCGGTTGTTAGAAGACCGATAGCAGGTTCGACGATTCCATACTGCCCAATGGGGAAGAGAATGGCAGATTGTTGGTCACCTCTTGAGCCAAACACATTCAAAGTCCGGGGAAAGAATTATCTTAGGGACAAAAAGAAAGAGTTAGCTCCCAATTGCGCTGCGTATTATCCTTTCGGGGTTGATATTTTCTTGTCTCAGAGGAAGATTGATCATATCGCTCGCTTTGTGGAACTTCCTGTTGTGAATGTAGATGGAGATGTCCCTTCTATTCTTGTTGTAAATGTTCAGATACCTCTGTATCCTATCTCTATGTTTCAAAGCGAACATGATGGAGAAGGAATGAATTTGGTCATGTATTTTAAGCTTTCTGAAAGTTACTCAAAAGACCTTCCATCTCATTTCCGCGATAGTTTAACTAAATTCATCAACGATGAAGTGGAGAGAGTTAGAGGATTCCCTGTTGATACAATTGCACCCTTCAGGGAAAGATTGAAAATATTGGGCCGAATAGTAAATATGGATGAACTCAGCAAATTGAATTTAAACACAGCTGAGAGGAAGCTAATGAATGCATGCAACGAAAAACCTGTCCTTTCACGCCCTCAACATGAATTTTACTTGGGAGAAAACTACTTTGAGATTGATTTAGATTTGCACAGATTCAGCTACGTATCCAGAAAGGGTATTGAATCATTTCATGAGAGAATGAAGCTATCAATACTGGATTTTGGTCTGACAATTCAGGGAAACAAGGCAGAAGAATTGCCCGAGCATATTTTATGCTGCGTACGGTTGGATGAACTTGATTACAATAATCGTTACCATCTAGGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

441

Amino Acids

49.83

Weight (kDa)

8.77

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 139 - 383 1.1e-55 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 500
AccII CGCG 3 cut(s) 155, 254, 891
AciI CCGC 6 cut(s) 66, 94, 311, 362, 488, 889
AclWI GGATC 2 cut(s) 92, 458
AcoI YGGCCR 1 cut(s) 67
AcsI RAATTY 4 cut(s) 829, 905, 1024, 1097
AcuI CTGAAG 2 cut(s) 156, 949
AfaI GTAC 2 cut(s) 169, 1275
AfiI CCNNNNNNNGG 1 cut(s) 509
AgsI TTSAA 5 cut(s) 586, 803, 979, 1024, 1175
AjnI CCWGG 1 cut(s) 482
AluBI AGCT 7 cut(s) 461, 632, 850, 1037, 1048, 1152, 1198
AluI AGCT 7 cut(s) 461, 632, 850, 1037, 1048, 1152, 1198
Alw26I GTCTC 1 cut(s) 684
AlwI GGATC 2 cut(s) 92, 458
Ama87I CYCGRG 1 cut(s) 1253
AoxI GGCC 4 cut(s) 20, 67, 398, 988
ApeKI GCWGC 8 cut(s) 437, 440, 443, 446, 449, 452, 644, 1269
ApoI RAATTY 4 cut(s) 829, 905, 1024, 1097
ArsI GACNNNNNNTTYG 2 cut(s) 1059, 1091
Asp700I GAANNNNTTC 1 cut(s) 1179
AspLEI GCGC 3 cut(s) 155, 254, 644
AspS9I GGNCC 2 cut(s) 398, 988
AsuC2I CCSGG 1 cut(s) 593
AsuHPI GGTGA 1 cut(s) 555
AvaI CYCGRG 1 cut(s) 1253
BaeI ACNNNNGTAYC 4 cut(s) 767, 767, 800, 800
BbsI GAAGAC 1 cut(s) 505
BbvI GCAGC 8 cut(s) 424, 427, 430, 433, 436, 439, 631, 1256
BccI CCATC 6 cut(s) 31, 172, 731, 809, 886, 1317
BceAI ACGGC 2 cut(s) 79, 385
BcgI CGANNNNNNTGC 1 cut(s) 31
BciT130I CCWGG 1 cut(s) 484
BciVI GTATCC 2 cut(s) 795, 1168
BclI TGATCA 1 cut(s) 694
BcnI CCSGG 1 cut(s) 593
BcoDI GTCTC 1 cut(s) 684
BfaI CTAG 3 cut(s) 267, 405, 1313
BfmI CTRYAG 1 cut(s) 208
BfuAI ACCTGC 1 cut(s) 500
BfuI GTATCC 2 cut(s) 795, 1168
BglI GCCNNNNNGGC 1 cut(s) 406
Bme1390I CCNGG 2 cut(s) 484, 593
BmeT110I CYCGRG 1 cut(s) 1253
BmgT120I GGNCC 2 cut(s) 398, 988
BmiI GGNNCC 1 cut(s) 399
BmrFI CCNGG 2 cut(s) 484, 593
BpiI GAAGAC 1 cut(s) 505
BplI GAGNNNNNCTC 2 cut(s) 340, 372
BpmI CTGGAG 2 cut(s) 342, 441
BpuEI CTTGAG 1 cut(s) 590
BpuMI CCSGG 1 cut(s) 593
BsaAI YACGTR 1 cut(s) 1156
BsaJI CCNNGG 4 cut(s) 136, 482, 483, 592
BsaXI ACNNNNNCTCC 5 cut(s) 27, 239, 269, 359, 389
Bsc4I CCNNNNNNNGG 1 cut(s) 509
Bse118I RCCGGY 1 cut(s) 72
Bse1I ACTGG 1 cut(s) 1212
BseBI CCWGG 1 cut(s) 484
BseDI CCNNGG 4 cut(s) 136, 482, 483, 592
BseGI GGATG 4 cut(s) 87, 289, 1012, 1288
BseLI CCNNNNNNNGG 1 cut(s) 509
BseMII CTCAG 3 cut(s) 695, 1027, 1029
BseNI ACTGG 1 cut(s) 1212
BseRI GAGGAG 2 cut(s) 97, 207
BseXI GCAGC 8 cut(s) 424, 427, 430, 433, 436, 439, 631, 1256
BseYI CCCAGC 1 cut(s) 30
Bsh1236I CGCG 3 cut(s) 155, 254, 891
BshFI GGCC 4 cut(s) 22, 69, 400, 990
BsiHKCI CYCGRG 1 cut(s) 1253
BsiSI CCGG 3 cut(s) 73, 91, 592
BsiWI CGTACG 1 cut(s) 1273
BslFI GGGAC 2 cut(s) 626, 731
BslI CCNNNNNNNGG 1 cut(s) 509
BsmAI GTCTC 1 cut(s) 684
BsmFI GGGAC 2 cut(s) 626, 731
BsmI GAATGC 2 cut(s) 303, 1060
BsnI GGCC 4 cut(s) 22, 69, 400, 990
BsoBI CYCGRG 1 cut(s) 1253
Bsp143I GATC 3 cut(s) 84, 463, 694
BspACI CCGC 6 cut(s) 66, 94, 311, 362, 488, 889
BspANI GGCC 4 cut(s) 22, 69, 400, 990
BspCNI CTCAG 3 cut(s) 694, 1026, 1030
BspFNI CGCG 3 cut(s) 155, 254, 891
BspHI TCATGA 1 cut(s) 1183
BspLI GGNNCC 1 cut(s) 399
BspMI ACCTGC 1 cut(s) 500
BspPI GGATC 2 cut(s) 92, 458
BsrFI RCCGGY 1 cut(s) 72
BsrI ACTGG 1 cut(s) 1212
BssAI RCCGGY 1 cut(s) 72
BssECI CCNNGG 4 cut(s) 136, 482, 483, 592
BssMI GATC 3 cut(s) 84, 463, 694
BssT1I CCWWGG 1 cut(s) 136
Bst2UI CCWGG 1 cut(s) 484
Bst4CI ACNGT 3 cut(s) 212, 242, 1278
Bst6I CTCTTC 3 cut(s) 195, 383, 536
BstBAI YACGTR 1 cut(s) 1156
BstC8I GCNNGC 4 cut(s) 332, 435, 706, 1060
BstDEI CTNAG 4 cut(s) 608, 681, 1013, 1038
BstEII GGTNACC 1 cut(s) 561
BstF5I GGATG 4 cut(s) 87, 289, 1012, 1288
BstFNI CGCG 3 cut(s) 155, 254, 891
BstHHI GCGC 3 cut(s) 155, 254, 644
BstKTI GATC 3 cut(s) 87, 466, 697
BstMAI GTCTC 1 cut(s) 684
BstMBI GATC 3 cut(s) 84, 463, 694
BstMWI GCNNNNNNNGC 6 cut(s) 406, 443, 446, 449, 452, 458
BstNI CCWGG 1 cut(s) 484
BstNSI RCATGY 3 cut(s) 12, 437, 1062
BstPI GGTNACC 1 cut(s) 561
BstSCI CCNGG 2 cut(s) 482, 591
BstSFI CTRYAG 1 cut(s) 208
BstSNI TACGTA 1 cut(s) 1156
BstUI CGCG 3 cut(s) 155, 254, 891
BstV1I GCAGC 8 cut(s) 424, 427, 430, 433, 436, 439, 631, 1256
BstV2I GAAGAC 1 cut(s) 505
BsuI GTATCC 2 cut(s) 795, 1168
BsuRI GGCC 4 cut(s) 22, 69, 400, 990
BtgZI GCGATG 1 cut(s) 50
BtsCI GGATG 4 cut(s) 87, 289, 1012, 1288
BtsIMutI CAGTG 2 cut(s) 180, 217
BveI ACCTGC 1 cut(s) 500
Cac8I GCNNGC 4 cut(s) 332, 435, 706, 1060
CciI TCATGA 1 cut(s) 1183
CfoI GCGC 3 cut(s) 155, 254, 644
Cfr10I RCCGGY 1 cut(s) 72
Cfr13I GGNCC 2 cut(s) 398, 988
Csp6I GTAC 2 cut(s) 168, 1274
CviAII CATG 7 cut(s) 9, 434, 812, 838, 1059, 1094, 1184
CviQI GTAC 2 cut(s) 168, 1274
DdeI CTNAG 4 cut(s) 608, 681, 1013, 1038
DpnI GATC 3 cut(s) 86, 465, 696
DpnII GATC 3 cut(s) 84, 463, 694
DraI TTTAAA 2 cut(s) 237, 1029
EaeI YGGCCR 1 cut(s) 67
Eam1104I CTCTTC 3 cut(s) 195, 383, 536
EarI CTCTTC 3 cut(s) 195, 383, 536
Eco105I TACGTA 1 cut(s) 1156
Eco130I CCWWGG 1 cut(s) 136
Eco57I CTGAAG 2 cut(s) 156, 949
Eco88I CYCGRG 1 cut(s) 1253
Eco91I GGTNACC 1 cut(s) 561
EcoO65I GGTNACC 1 cut(s) 561
EcoRII CCWGG 1 cut(s) 482
EcoT14I CCWWGG 1 cut(s) 136
EcoT22I ATGCAT 1 cut(s) 1060
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 7 cut(s) 12, 437, 815, 841, 1062, 1097, 1187
FaqI GGGAC 2 cut(s) 626, 731
FatI CATG 7 cut(s) 8, 433, 811, 837, 1058, 1093, 1183
FbaI TGATCA 1 cut(s) 694
FokI GGATG 4 cut(s) 74, 296, 1019, 1295
FspBI CTAG 3 cut(s) 267, 405, 1313
GlaI GCGC 3 cut(s) 154, 253, 643
GsaI CCCAGC 1 cut(s) 34
GsuI CTGGAG 2 cut(s) 342, 441
HaeIII GGCC 4 cut(s) 22, 69, 400, 990
HapII CCGG 3 cut(s) 73, 91, 592
HhaI GCGC 3 cut(s) 155, 254, 644
Hin1II CATG 7 cut(s) 12, 437, 815, 841, 1062, 1097, 1187
Hin6I GCGC 3 cut(s) 153, 252, 642
HinP1I GCGC 3 cut(s) 153, 252, 642
HindIII AAGCTT 1 cut(s) 848
HinfI GANTC 5 cut(s) 182, 520, 939, 1146, 1175
HpaII CCGG 3 cut(s) 73, 91, 592
HphI GGTGA 1 cut(s) 555
Hpy188I TCNGA 8 cut(s) 187, 247, 321, 387, 684, 774, 856, 1221
Hpy188III TCNNGA 3 cut(s) 569, 1161, 1184
Hpy99I CGWCG 1 cut(s) 520
HpyAV CCTTC 7 cut(s) 19, 354, 422, 759, 815, 884, 973
HpyCH4III ACNGT 3 cut(s) 212, 242, 1278
HpyCH4IV ACGT 1 cut(s) 1155
HpyCH4V TGCA 5 cut(s) 301, 959, 1058, 1062, 1141
HpyF10VI GCNNNNNNNGC 6 cut(s) 406, 443, 446, 449, 452, 458
HpyF3I CTNAG 4 cut(s) 608, 681, 1013, 1038
HpySE526I ACGT 1 cut(s) 1155
Hsp92II CATG 7 cut(s) 12, 437, 815, 841, 1062, 1097, 1187
HspAI GCGC 3 cut(s) 153, 252, 642
Ksp22I TGATCA 1 cut(s) 694
Kzo9I GATC 3 cut(s) 84, 463, 694
LmnI GCTCC 4 cut(s) 5, 187, 460, 637
Lsp1109I GCAGC 8 cut(s) 424, 427, 430, 433, 436, 439, 631, 1256
MaeI CTAG 3 cut(s) 267, 405, 1313
MaeII ACGT 1 cut(s) 1155
MaeIII GTNAC 5 cut(s) 255, 290, 561, 860, 1304
MalI GATC 3 cut(s) 86, 465, 696
MboI GATC 3 cut(s) 84, 463, 694
MboII GAAGA 9 cut(s) 64, 67, 70, 212, 400, 510, 553, 700, 1256
MfeI CAATTG 2 cut(s) 637, 954
MmeI TCCRAC 2 cut(s) 135, 1260
Mph1103I ATGCAT 1 cut(s) 1060
MroXI GAANNNNTTC 1 cut(s) 1179
MseI TTAA 5 cut(s) 236, 417, 846, 899, 1028
MspA1I CMGCKG 3 cut(s) 362, 461, 1037
MspI CCGG 3 cut(s) 73, 91, 592
MspR9I CCNGG 2 cut(s) 484, 593
MunI CAATTG 2 cut(s) 637, 954
Mva1269I GAATGC 2 cut(s) 303, 1060
MvaI CCWGG 1 cut(s) 484
MvnI CGCG 3 cut(s) 155, 254, 891
MwoI GCNNNNNNNGC 6 cut(s) 406, 443, 446, 449, 452, 458
NciI CCSGG 1 cut(s) 593
NdeII GATC 3 cut(s) 84, 463, 694
NlaIII CATG 7 cut(s) 12, 437, 815, 841, 1062, 1097, 1187
NlaIV GGNNCC 1 cut(s) 399
NmuCI GTSAC 2 cut(s) 290, 561
NsiI ATGCAT 1 cut(s) 1060
NspI RCATGY 3 cut(s) 12, 437, 1062
PaeI GCATGC 2 cut(s) 437, 1062
PagI TCATGA 1 cut(s) 1183
PasI CCCWGGG 1 cut(s) 483
PcsI WCGNNNNNNNCGW 1 cut(s) 251
PctI GAATGC 2 cut(s) 303, 1060
PdmI GAANNNNTTC 1 cut(s) 1179
PfeI GAWTC 5 cut(s) 182, 520, 939, 1146, 1175
Pfl23II CGTACG 1 cut(s) 1273
Ppu21I YACGTR 1 cut(s) 1156
Psp6I CCWGG 1 cut(s) 482
PspEI GGTNACC 1 cut(s) 561
PspFI CCCAGC 1 cut(s) 30
PspGI CCWGG 1 cut(s) 482
PspLI CGTACG 1 cut(s) 1273
PspN4I GGNNCC 1 cut(s) 399
PspPI GGNCC 2 cut(s) 398, 988
PvuII CAGCTG 2 cut(s) 461, 1037
RsaI GTAC 2 cut(s) 169, 1275
RsaNI GTAC 2 cut(s) 168, 1274
SaqAI TTAA 5 cut(s) 236, 417, 846, 899, 1028
Sau3AI GATC 3 cut(s) 84, 463, 694
Sau96I GGNCC 2 cut(s) 398, 988
ScrFI CCNGG 2 cut(s) 484, 593
SfcI CTRYAG 1 cut(s) 208
SmlI CTYRAG 1 cut(s) 569
SmoI CTYRAG 1 cut(s) 569
SnaBI TACGTA 1 cut(s) 1156
SphI GCATGC 2 cut(s) 437, 1062
SsiI CCGC 6 cut(s) 66, 94, 311, 362, 488, 889
SspI AATATT 1 cut(s) 984
SspMI CTAG 3 cut(s) 267, 405, 1313
StyD4I CCNGG 2 cut(s) 482, 591
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 3 cut(s) 212, 242, 1278
TaiI ACGT 1 cut(s) 1158
TaqI TCGA 3 cut(s) 17, 347, 515
TaqII GACCGA 1 cut(s) 517
TatI WGTACW 1 cut(s) 167
TauI GCSGC 2 cut(s) 69, 97
TfiI GAWTC 5 cut(s) 182, 520, 939, 1146, 1175
Tru1I TTAA 5 cut(s) 236, 417, 846, 899, 1028
Tru9I TTAA 5 cut(s) 236, 417, 846, 899, 1028
TscAI CASTG 2 cut(s) 180, 217
TseFI GTSAC 2 cut(s) 290, 561
TseI GCWGC 8 cut(s) 437, 440, 443, 446, 449, 452, 644, 1269
Tsp45I GTSAC 2 cut(s) 290, 561
TspGWI ACGGA 1 cut(s) 97
TspRI CASTG 2 cut(s) 180, 217
XapI RAATTY 4 cut(s) 829, 905, 1024, 1097
XceI RCATGY 3 cut(s) 12, 437, 1062
XmnI GAANNNNTTC 1 cut(s) 1179
XspI CTAG 3 cut(s) 267, 405, 1313
Zsp2I ATGCAT 1 cut(s) 1060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.