Rw2G050930

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0011589
Physical Location & Seq
Reverse (-)
78634649 .. 78637981
3333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G050930.1

Sequence Viewer

Length: 1305 bp
ATGGGAGCTTGCGCTTCCCGGCCCAAGGGCTGGGCCTTGAGATTGTCAAAGAAGAAGAAGAATGGCCGCCAAAGACGAATCATTCGGAGGCGTGCTTCCTCCTCCAACCGATCTAACTTCAACCCACAGCTCCAAGGAAGCATGGACGGGGCTTGGTTTGATTGTACTTCAGTGATGGATTCGGAGCGAGGAGACGAAGAGTTCTACAGTGTCTATGATGATGTGGTGTCTTTGAATGGTTCGGAGAGTGCGATACAGAGAACGAGGGAAGGCGGTGCGGATGAGAGTGATAGAATGCAAGGGTTGGTGGTTGTTCAGAATGCTTGCCTGCCTTGTCTACCTTCAACTAGCCCCTTGTCAGTGGATAACAAACTGAGTTTTAGGAAGAGATTGGGGCCTTCTAGGCTTTCGTTTAAGTGGAGGGAAGGGCATGCTGCTCCGGCTGATCCCACATTAGTTTCGCCCAGGGCGGTTGTTAAAAGACCAATAGCAGGTTCGACAATTCCACACTGCCCGATGGGCAAGAGAATGCCAGATTGTTGGTCACCTCTTGAGCCAAACACTTTCAAAGTCCGGGGGAAGAATTATCTTAGGGACAAAAAGAAAGAGTTAGCTCCAGATTGCGCCGCGTATTATCCTTTTGGGGTTGATATTTTTTTATCTCAGAGGAAGATTGATCATATTGCTCGCTTTGTGGAACTTCCTGTTGTGAATGTAGATGGAGATGTCCCTTCTATTCTTGTTGTAAATGTTCAGATACCTTTGTACCCTATCTCTATGTTTCAGAGCGAACATGATGGAGAAGGAATGAATTTGGTTATGTATTTTAAGCTTTCTGAAAGTTACTCAAAAGACCTTCCAGCTCATTTCCGCGAAAGTTTAACTAAATTCATCAATGATGAAGTGGAAAGAGTTAGAGGATTCCCTGTTGATACAATTGCACCCTTCAGGGAAAGATTGAAAATATTGGGCCGAATTGTAAATGTGGATGAACTCAGCAAACTGAATTTAAATGCAGCTGAGAGGAGGCTAATGAATGCATGCAATGAAAAACCTGTCCTTTCACGCCCTCAACATGAATTTTACTTGGGAGAAAACTACTTTGAGATTGATTTGGATTTGCACAGATTCAGCTATGTGTCTAGAAAGGGTATTGAATCATTTCATGAGAGAATGAAGCTATCCATACTGGATTTTGGTCTGACAATTCAGGGGAATAAGGCAGAAGAATTGCCCGAGCATATTTTATGCTGCGTACGGTTGGATGAACTTGACTACCATAATCGATACAACCTGGGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

434

Amino Acids

49.43

Weight (kDa)

8.79

Isoelectric Point (pI)

51.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 181 - 425 2.7e-55 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 482
AccB7I CCANNNNNTGG 1 cut(s) 30
AccI GTMKAC 1 cut(s) 337
AccII CGCG 2 cut(s) 629, 873
AciI CCGC 6 cut(s) 67, 273, 278, 470, 627, 871
AclWI GGATC 1 cut(s) 440
AcoI YGGCCR 1 cut(s) 64
AcsI RAATTY 4 cut(s) 811, 887, 1006, 1079
AcuI CTGAAG 2 cut(s) 153, 931
AfaI GTAC 3 cut(s) 166, 767, 1257
AfiI CCNNNNNNNGG 3 cut(s) 25, 30, 491
AgsI TTSAA 6 cut(s) 121, 235, 345, 568, 961, 1157
AjnI CCWGG 2 cut(s) 464, 1293
AjuI GAANNNNNNNTTGG 2 cut(s) 478, 510
AluBI AGCT 8 cut(s) 8, 130, 614, 832, 863, 1019, 1134, 1180
AluI AGCT 8 cut(s) 8, 130, 614, 832, 863, 1019, 1134, 1180
Alw26I GTCTC 1 cut(s) 186
AlwI GGATC 1 cut(s) 440
Ama87I CYCGRG 1 cut(s) 1235
AoxI GGCC 5 cut(s) 20, 33, 64, 395, 970
ApeKI GCWGC 3 cut(s) 434, 1016, 1251
ApoI RAATTY 4 cut(s) 811, 887, 1006, 1079
ArsI GACNNNNNNTTYG 2 cut(s) 66, 98
Asp700I GAANNNNTTC 1 cut(s) 1161
AspLEI GCGC 2 cut(s) 14, 626
AspS9I GGNCC 4 cut(s) 21, 33, 395, 970
AsuC2I CCSGG 2 cut(s) 19, 575
AsuHPI GGTGA 1 cut(s) 537
AvaI CYCGRG 1 cut(s) 1235
BaeI ACNNNNGTAYC 4 cut(s) 749, 749, 782, 782
BbvI GCAGC 3 cut(s) 421, 1028, 1238
BccI CCATC 4 cut(s) 169, 511, 713, 791
BciT130I CCWGG 2 cut(s) 466, 1295
BclI TGATCA 1 cut(s) 676
BcnI CCSGG 2 cut(s) 19, 575
BcoDI GTCTC 1 cut(s) 186
BfaI CTAG 3 cut(s) 348, 402, 1143
BfmI CTRYAG 1 cut(s) 205
BfuAI ACCTGC 1 cut(s) 482
BglI GCCNNNNNGGC 2 cut(s) 403, 519
BisI GCNGC 5 cut(s) 67, 435, 627, 1017, 1252
BlsI GCNGC 5 cut(s) 68, 436, 628, 1018, 1253
Bme1390I CCNGG 4 cut(s) 19, 466, 575, 1295
BmeT110I CYCGRG 1 cut(s) 1235
BmgT120I GGNCC 4 cut(s) 21, 33, 395, 970
BmiI GGNNCC 1 cut(s) 396
BmrFI CCNGG 4 cut(s) 19, 466, 575, 1295
BpmI CTGGAG 1 cut(s) 600
BpuEI CTTGAG 2 cut(s) 58, 572
BpuMI CCSGG 2 cut(s) 19, 575
Bsa29I ATCGAT 1 cut(s) 1285
BsaJI CCNNGG 6 cut(s) 24, 133, 464, 465, 574, 1294
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 30, 491
Bse1I ACTGG 1 cut(s) 1194
Bse3DI GCAATG 1 cut(s) 1051
BseBI CCWGG 2 cut(s) 466, 1295
BseCI ATCGAT 1 cut(s) 1285
BseDI CCNNGG 6 cut(s) 24, 133, 464, 465, 574, 1294
BseGI GGATG 3 cut(s) 286, 994, 1270
BseLI CCNNNNNNNGG 3 cut(s) 25, 30, 491
BseMI GCAATG 1 cut(s) 1051
BseMII CTCAG 4 cut(s) 365, 677, 1009, 1011
BseNI ACTGG 1 cut(s) 1194
BseRI GAGGAG 3 cut(s) 91, 204, 1039
BseXI GCAGC 3 cut(s) 421, 1028, 1238
BseYI CCCAGC 1 cut(s) 30
Bsh1236I CGCG 2 cut(s) 629, 873
BshFI GGCC 5 cut(s) 22, 35, 66, 397, 972
BshVI ATCGAT 1 cut(s) 1285
BsiHKCI CYCGRG 1 cut(s) 1235
BsiSI CCGG 3 cut(s) 19, 440, 574
BsiWI CGTACG 1 cut(s) 1255
BslFI GGGAC 2 cut(s) 608, 713
BslI CCNNNNNNNGG 3 cut(s) 25, 30, 491
BsmAI GTCTC 1 cut(s) 186
BsmBI CGTCTC 1 cut(s) 186
BsmFI GGGAC 2 cut(s) 608, 713
BsmI GAATGC 4 cut(s) 300, 325, 534, 1042
BsnI GGCC 5 cut(s) 22, 35, 66, 397, 972
BsoBI CYCGRG 1 cut(s) 1235
Bsp143I GATC 3 cut(s) 110, 445, 676
BspACI CCGC 6 cut(s) 67, 273, 278, 470, 627, 871
BspANI GGCC 5 cut(s) 22, 35, 66, 397, 972
BspCNI CTCAG 4 cut(s) 366, 676, 1008, 1012
BspDI ATCGAT 1 cut(s) 1285
BspFNI CGCG 2 cut(s) 629, 873
BspHI TCATGA 1 cut(s) 1165
BspLI GGNNCC 1 cut(s) 396
BspMI ACCTGC 1 cut(s) 482
BspPI GGATC 1 cut(s) 440
BsrDI GCAATG 1 cut(s) 1051
BsrI ACTGG 1 cut(s) 1194
BssECI CCNNGG 6 cut(s) 24, 133, 464, 465, 574, 1294
BssMI GATC 3 cut(s) 110, 445, 676
BssT1I CCWWGG 2 cut(s) 24, 133
Bst2UI CCWGG 2 cut(s) 466, 1295
Bst4CI ACNGT 2 cut(s) 209, 1260
Bst6I CTCTTC 2 cut(s) 192, 380
BstC8I GCNNGC 7 cut(s) 10, 93, 325, 329, 432, 688, 1042
BstDEI CTNAG 5 cut(s) 374, 590, 663, 995, 1020
BstEII GGTNACC 1 cut(s) 543
BstF5I GGATG 3 cut(s) 286, 994, 1270
BstFNI CGCG 2 cut(s) 629, 873
BstHHI GCGC 2 cut(s) 14, 626
BstKTI GATC 3 cut(s) 113, 448, 679
BstMAI GTCTC 1 cut(s) 186
BstMBI GATC 3 cut(s) 110, 445, 676
BstMWI GCNNNNNNNGC 3 cut(s) 403, 440, 519
BstNI CCWGG 2 cut(s) 466, 1295
BstNSI RCATGY 2 cut(s) 434, 1044
BstPI GGTNACC 1 cut(s) 543
BstSCI CCNGG 4 cut(s) 17, 464, 573, 1293
BstSFI CTRYAG 1 cut(s) 205
BstUI CGCG 2 cut(s) 629, 873
BstV1I GCAGC 3 cut(s) 421, 1028, 1238
BstXI CCANNNNNNTGG 1 cut(s) 540
Bsu15I ATCGAT 1 cut(s) 1285
BsuRI GGCC 5 cut(s) 22, 35, 66, 397, 972
BsuTUI ATCGAT 1 cut(s) 1285
BtsCI GGATG 3 cut(s) 286, 994, 1270
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 4 cut(s) 177, 214, 366, 508
BveI ACCTGC 1 cut(s) 482
Cac8I GCNNGC 7 cut(s) 10, 93, 325, 329, 432, 688, 1042
CciI TCATGA 1 cut(s) 1165
CfoI GCGC 2 cut(s) 14, 626
Cfr13I GGNCC 4 cut(s) 21, 33, 395, 970
ClaI ATCGAT 1 cut(s) 1285
Csp6I GTAC 3 cut(s) 165, 766, 1256
CviAII CATG 6 cut(s) 142, 431, 794, 1041, 1076, 1166
CviQI GTAC 3 cut(s) 165, 766, 1256
DdeI CTNAG 5 cut(s) 374, 590, 663, 995, 1020
DpnI GATC 3 cut(s) 112, 447, 678
DpnII GATC 3 cut(s) 110, 445, 676
DraI TTTAAA 1 cut(s) 1011
EaeI YGGCCR 1 cut(s) 64
Eam1104I CTCTTC 2 cut(s) 192, 380
EarI CTCTTC 2 cut(s) 192, 380
Eco130I CCWWGG 2 cut(s) 24, 133
Eco57I CTGAAG 2 cut(s) 153, 931
Eco88I CYCGRG 1 cut(s) 1235
Eco91I GGTNACC 1 cut(s) 543
EcoO109I RGGNCCY 1 cut(s) 395
EcoO65I GGTNACC 1 cut(s) 543
EcoRII CCWGG 2 cut(s) 464, 1293
EcoT14I CCWWGG 2 cut(s) 24, 133
EcoT22I ATGCAT 1 cut(s) 1042
ErhI CCWWGG 2 cut(s) 24, 133
Esp3I CGTCTC 1 cut(s) 186
FaeI CATG 6 cut(s) 145, 434, 797, 1044, 1079, 1169
FaqI GGGAC 2 cut(s) 608, 713
FatI CATG 6 cut(s) 141, 430, 793, 1040, 1075, 1165
FbaI TGATCA 1 cut(s) 676
FblI GTMKAC 1 cut(s) 337
Fnu4HI GCNGC 5 cut(s) 67, 435, 627, 1017, 1252
FokI GGATG 3 cut(s) 293, 1001, 1277
Fsp4HI GCNGC 5 cut(s) 67, 435, 627, 1017, 1252
FspBI CTAG 3 cut(s) 348, 402, 1143
GlaI GCGC 2 cut(s) 13, 625
GluI GCNGC 5 cut(s) 67, 435, 627, 1017, 1252
GsaI CCCAGC 1 cut(s) 34
GsuI CTGGAG 1 cut(s) 600
HaeIII GGCC 5 cut(s) 22, 35, 66, 397, 972
HapII CCGG 3 cut(s) 19, 440, 574
HhaI GCGC 2 cut(s) 14, 626
Hin1II CATG 6 cut(s) 145, 434, 797, 1044, 1079, 1169
Hin6I GCGC 2 cut(s) 12, 624
HinP1I GCGC 2 cut(s) 12, 624
HindIII AAGCTT 1 cut(s) 830
HinfI GANTC 5 cut(s) 78, 179, 921, 1128, 1157
HpaII CCGG 3 cut(s) 19, 440, 574
HphI GGTGA 1 cut(s) 537
Hpy166II GTNNAC 1 cut(s) 338
Hpy188I TCNGA 9 cut(s) 87, 184, 244, 318, 666, 756, 786, 838, 1203
Hpy188III TCNNGA 4 cut(s) 551, 617, 1143, 1166
Hpy8I GTNNAC 1 cut(s) 338
HpyAV CCTTC 8 cut(s) 263, 351, 408, 419, 741, 797, 866, 955
HpyCH4III ACNGT 2 cut(s) 209, 1260
HpyCH4V TGCA 6 cut(s) 298, 941, 1016, 1040, 1044, 1123
HpyF10VI GCNNNNNNNGC 3 cut(s) 403, 440, 519
HpyF3I CTNAG 5 cut(s) 374, 590, 663, 995, 1020
Hsp92II CATG 6 cut(s) 145, 434, 797, 1044, 1079, 1169
HspAI GCGC 2 cut(s) 12, 624
Ksp22I TGATCA 1 cut(s) 676
Kzo9I GATC 3 cut(s) 110, 445, 676
LmnI GCTCC 5 cut(s) 5, 135, 184, 442, 619
Lsp1109I GCAGC 3 cut(s) 421, 1028, 1238
MaeI CTAG 3 cut(s) 348, 402, 1143
MaeIII GTNAC 2 cut(s) 543, 842
MalI GATC 3 cut(s) 112, 447, 678
MboI GATC 3 cut(s) 110, 445, 676
MboII GAAGA 8 cut(s) 64, 67, 70, 209, 397, 592, 682, 1238
MfeI CAATTG 1 cut(s) 936
MmeI TCCRAC 2 cut(s) 129, 1242
Mph1103I ATGCAT 1 cut(s) 1042
MroXI GAANNNNTTC 1 cut(s) 1161
MseI TTAA 5 cut(s) 414, 477, 828, 881, 1010
MspA1I CMGCKG 1 cut(s) 1019
MspI CCGG 3 cut(s) 19, 440, 574
MspR9I CCNGG 4 cut(s) 19, 466, 575, 1295
MunI CAATTG 1 cut(s) 936
Mva1269I GAATGC 4 cut(s) 300, 325, 534, 1042
MvaI CCWGG 2 cut(s) 466, 1295
MvnI CGCG 2 cut(s) 629, 873
MwoI GCNNNNNNNGC 3 cut(s) 403, 440, 519
NciI CCSGG 2 cut(s) 19, 575
NdeII GATC 3 cut(s) 110, 445, 676
NlaIII CATG 6 cut(s) 145, 434, 797, 1044, 1079, 1169
NlaIV GGNNCC 1 cut(s) 396
NmuCI GTSAC 1 cut(s) 543
NsiI ATGCAT 1 cut(s) 1042
NspI RCATGY 2 cut(s) 434, 1044
PaeI GCATGC 2 cut(s) 434, 1044
PagI TCATGA 1 cut(s) 1165
PasI CCCWGGG 1 cut(s) 465
PcsI WCGNNNNNNNCGW 1 cut(s) 248
PctI GAATGC 4 cut(s) 300, 325, 534, 1042
PdmI GAANNNNTTC 1 cut(s) 1161
PfeI GAWTC 5 cut(s) 78, 179, 921, 1128, 1157
Pfl23II CGTACG 1 cut(s) 1255
PflMI CCANNNNNTGG 1 cut(s) 30
PkrI GCNGC 5 cut(s) 68, 436, 628, 1018, 1253
Psp6I CCWGG 2 cut(s) 464, 1293
PspEI GGTNACC 1 cut(s) 543
PspFI CCCAGC 1 cut(s) 30
PspGI CCWGG 2 cut(s) 464, 1293
PspLI CGTACG 1 cut(s) 1255
PspN4I GGNNCC 1 cut(s) 396
PspPI GGNCC 4 cut(s) 21, 33, 395, 970
PvuII CAGCTG 1 cut(s) 1019
RsaI GTAC 3 cut(s) 166, 767, 1257
RsaNI GTAC 3 cut(s) 165, 766, 1256
SaqAI TTAA 5 cut(s) 414, 477, 828, 881, 1010
SatI GCNGC 5 cut(s) 67, 435, 627, 1017, 1252
Sau3AI GATC 3 cut(s) 110, 445, 676
Sau96I GGNCC 4 cut(s) 21, 33, 395, 970
ScrFI CCNGG 4 cut(s) 19, 466, 575, 1295
SfcI CTRYAG 1 cut(s) 205
SmiI ATTTAAAT 1 cut(s) 1011
SmlI CTYRAG 2 cut(s) 37, 551
SmoI CTYRAG 2 cut(s) 37, 551
SphI GCATGC 2 cut(s) 434, 1044
SsiI CCGC 6 cut(s) 67, 273, 278, 470, 627, 871
SspI AATATT 1 cut(s) 966
SspMI CTAG 3 cut(s) 348, 402, 1143
StyD4I CCNGG 4 cut(s) 17, 464, 573, 1293
StyI CCWWGG 2 cut(s) 24, 133
SwaI ATTTAAAT 1 cut(s) 1011
TaaI ACNGT 2 cut(s) 209, 1260
TaqI TCGA 2 cut(s) 497, 1285
TatI WGTACW 1 cut(s) 164
TauI GCSGC 2 cut(s) 69, 629
TfiI GAWTC 5 cut(s) 78, 179, 921, 1128, 1157
Tru1I TTAA 5 cut(s) 414, 477, 828, 881, 1010
Tru9I TTAA 5 cut(s) 414, 477, 828, 881, 1010
TscAI CASTG 4 cut(s) 177, 214, 366, 515
TseFI GTSAC 1 cut(s) 543
TseI GCWGC 3 cut(s) 434, 1016, 1251
Tsp45I GTSAC 1 cut(s) 543
TspRI CASTG 4 cut(s) 177, 214, 366, 515
Van91I CCANNNNNTGG 1 cut(s) 30
XapI RAATTY 4 cut(s) 811, 887, 1006, 1079
XbaI TCTAGA 1 cut(s) 1142
XceI RCATGY 2 cut(s) 434, 1044
XmiI GTMKAC 1 cut(s) 337
XmnI GAANNNNTTC 1 cut(s) 1161
XspI CTAG 3 cut(s) 348, 402, 1143
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.