Rh2DG637500

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011589
Physical Location & Seq
Reverse (-)
86608477 .. 86611190
2714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG637500.1

Sequence Viewer

Length: 1308 bp
ATGGGAGCTTGCGCTTCCCGGCCCAAGGGCTGGGCCTTGAGATTGTCAAAGAAGAAGAAGAATGGCCGCCAAAGACGAATCATTCGGAGGCGTGTTTCCTCCTCCAACCGATCTAACTTATTCAACCCACAACTCCAAGGAAGCATGGACGGGGCTTGGTTTGATTGTACTTCAGTGATGGATTCGGAGCGAGGAGACGAAGAGTTCTACAGTGTCTATGATGATGTGGTGTCTTTGAATGGTTCGGAGAGTGCGATACAGAGAACGAGGGAAGGCGGTGCGGATGAGAGTGATAGAATGCAAGGGTTGGTGGTTGTTCAGAATGCTTGCCTGCCTTGTCTACCTTCAACTAGCCCCTTGTCAGTTGATAACAAACTGAGTTTTAGGAAGAGATTGGGGCCTTCTAGGCTTTCGTTTAAGTGGAGGGAAGGGCATGCTGCTCCGGCTGATCCCACATTAGTTTCGCCCAGGGCGGTTGTTAAAAGACCAATAGCAGGTTCGACAATTCCACACTGCCCGATGGGCAAGAGAATGCCAGATTGTTGGTCACCTCTTGAGCCAAACACTTTCAAAGTCCGGGGGAAGAATTATCTTAGGGACAAAAAGAAAGAGTTAGCTCCAGATTGCGCCGCGTATTATCCTTTTGGGGTTGATATTTTTTTATCTCAGAGGAAGATTGATCATATTGCTCGCTTTGTGGAACTTCCTGTTGTGAATGTAGATGGAGATGTCCCTTCTATTCTTGTTGTAAATGTTCAGATACCTTTGTACCCTATCTCTATGTTTCAGAGCGAACATGATGGAGAAGGAATGAATTTGGTTATGTATTTTAAGCTTTCTGAAAGTTACTCAAAAGACCTTCCAGCTCATTTCCGCGAAAGTTTAACTAAATTCATCAATGATGAAGTGGAAAGAGTTAGAGGATTCCCTGTTGATACAATTGCACCCTTCAGGGAAAGATTGAAAATATTGGGCCGAATTGTAAATGTGGATGAACTCAGCAAACTGAATTTAAACGCAGCTGAGAGGAGGCTAATGAATGCATGCAACGAAAAACCTGTCCTTTCACGCCCTCAACATGAATTTTACTTGGGAGAAAACTACTTTGAGATTGATTTGGATTTGCACAGATTCAGCTATGTGTCTAGAAAGGGTATTGAATCATTTCATGAGAGAATGAAGCTATCCATACTGGATTTTGGTCTGACAATTCAGGGGAATAAGGCAGAAGAATTGCCCGAGCATATTTTATGCTGCGTACGGTTGGATGAACTTGACTACCATAATCGATACAACCTGGGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

435

Amino Acids

49.58

Weight (kDa)

8.79

Isoelectric Point (pI)

51.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 182 - 426 2.8e-55 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 485
AccB7I CCANNNNNTGG 1 cut(s) 30
AccI GTMKAC 1 cut(s) 340
AccII CGCG 2 cut(s) 632, 876
AciI CCGC 6 cut(s) 67, 276, 281, 473, 630, 874
AclWI GGATC 1 cut(s) 443
AcoI YGGCCR 1 cut(s) 64
AcsI RAATTY 4 cut(s) 814, 890, 1009, 1082
AcuI CTGAAG 2 cut(s) 156, 934
AfaI GTAC 3 cut(s) 169, 770, 1260
AfiI CCNNNNNNNGG 3 cut(s) 25, 30, 494
AgsI TTSAA 6 cut(s) 124, 238, 348, 571, 964, 1160
AjnI CCWGG 2 cut(s) 467, 1296
AjuI GAANNNNNNNTTGG 2 cut(s) 481, 513
AluBI AGCT 7 cut(s) 8, 617, 835, 866, 1022, 1137, 1183
AluI AGCT 7 cut(s) 8, 617, 835, 866, 1022, 1137, 1183
Alw26I GTCTC 1 cut(s) 189
AlwI GGATC 1 cut(s) 443
Ama87I CYCGRG 1 cut(s) 1238
AoxI GGCC 5 cut(s) 20, 33, 64, 398, 973
ApeKI GCWGC 3 cut(s) 437, 1019, 1254
ApoI RAATTY 4 cut(s) 814, 890, 1009, 1082
ArsI GACNNNNNNTTYG 4 cut(s) 66, 98, 1044, 1076
Asp700I GAANNNNTTC 1 cut(s) 1164
AspLEI GCGC 2 cut(s) 14, 629
AspS9I GGNCC 4 cut(s) 21, 33, 398, 973
AsuC2I CCSGG 2 cut(s) 19, 578
AsuHPI GGTGA 1 cut(s) 540
AvaI CYCGRG 1 cut(s) 1238
BaeI ACNNNNGTAYC 4 cut(s) 752, 752, 785, 785
BbvI GCAGC 3 cut(s) 424, 1031, 1241
BccI CCATC 4 cut(s) 172, 514, 716, 794
BciT130I CCWGG 2 cut(s) 469, 1298
BclI TGATCA 1 cut(s) 679
BcnI CCSGG 2 cut(s) 19, 578
BcoDI GTCTC 1 cut(s) 189
BfaI CTAG 3 cut(s) 351, 405, 1146
BfmI CTRYAG 1 cut(s) 208
BfuAI ACCTGC 1 cut(s) 485
BglI GCCNNNNNGGC 2 cut(s) 406, 522
BisI GCNGC 5 cut(s) 67, 438, 630, 1020, 1255
BlsI GCNGC 5 cut(s) 68, 439, 631, 1021, 1256
Bme1390I CCNGG 4 cut(s) 19, 469, 578, 1298
BmeT110I CYCGRG 1 cut(s) 1238
BmgT120I GGNCC 4 cut(s) 21, 33, 398, 973
BmiI GGNNCC 1 cut(s) 399
BmrFI CCNGG 4 cut(s) 19, 469, 578, 1298
BpmI CTGGAG 1 cut(s) 603
BpuEI CTTGAG 2 cut(s) 58, 575
BpuMI CCSGG 2 cut(s) 19, 578
Bsa29I ATCGAT 1 cut(s) 1288
BsaJI CCNNGG 6 cut(s) 24, 136, 467, 468, 577, 1297
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 30, 494
Bse1I ACTGG 1 cut(s) 1197
BseBI CCWGG 2 cut(s) 469, 1298
BseCI ATCGAT 1 cut(s) 1288
BseDI CCNNGG 6 cut(s) 24, 136, 467, 468, 577, 1297
BseGI GGATG 3 cut(s) 289, 997, 1273
BseLI CCNNNNNNNGG 3 cut(s) 25, 30, 494
BseMII CTCAG 4 cut(s) 368, 680, 1012, 1014
BseNI ACTGG 1 cut(s) 1197
BseRI GAGGAG 3 cut(s) 91, 207, 1042
BseXI GCAGC 3 cut(s) 424, 1031, 1241
BseYI CCCAGC 1 cut(s) 30
Bsh1236I CGCG 2 cut(s) 632, 876
BshFI GGCC 5 cut(s) 22, 35, 66, 400, 975
BshVI ATCGAT 1 cut(s) 1288
BsiHKCI CYCGRG 1 cut(s) 1238
BsiSI CCGG 3 cut(s) 19, 443, 577
BsiWI CGTACG 1 cut(s) 1258
BslFI GGGAC 2 cut(s) 611, 716
BslI CCNNNNNNNGG 3 cut(s) 25, 30, 494
BsmAI GTCTC 1 cut(s) 189
BsmBI CGTCTC 1 cut(s) 189
BsmFI GGGAC 2 cut(s) 611, 716
BsmI GAATGC 4 cut(s) 303, 328, 537, 1045
BsnI GGCC 5 cut(s) 22, 35, 66, 400, 975
BsoBI CYCGRG 1 cut(s) 1238
Bsp143I GATC 3 cut(s) 110, 448, 679
BspACI CCGC 6 cut(s) 67, 276, 281, 473, 630, 874
BspANI GGCC 5 cut(s) 22, 35, 66, 400, 975
BspCNI CTCAG 4 cut(s) 369, 679, 1011, 1015
BspDI ATCGAT 1 cut(s) 1288
BspFNI CGCG 2 cut(s) 632, 876
BspHI TCATGA 1 cut(s) 1168
BspLI GGNNCC 1 cut(s) 399
BspMI ACCTGC 1 cut(s) 485
BspPI GGATC 1 cut(s) 443
BsrI ACTGG 1 cut(s) 1197
BssECI CCNNGG 6 cut(s) 24, 136, 467, 468, 577, 1297
BssMI GATC 3 cut(s) 110, 448, 679
BssT1I CCWWGG 2 cut(s) 24, 136
Bst2UI CCWGG 2 cut(s) 469, 1298
Bst4CI ACNGT 2 cut(s) 212, 1263
Bst6I CTCTTC 2 cut(s) 195, 383
BstC8I GCNNGC 6 cut(s) 10, 328, 332, 435, 691, 1045
BstDEI CTNAG 5 cut(s) 377, 593, 666, 998, 1023
BstEII GGTNACC 1 cut(s) 546
BstF5I GGATG 3 cut(s) 289, 997, 1273
BstFNI CGCG 2 cut(s) 632, 876
BstHHI GCGC 2 cut(s) 14, 629
BstKTI GATC 3 cut(s) 113, 451, 682
BstMAI GTCTC 1 cut(s) 189
BstMBI GATC 3 cut(s) 110, 448, 679
BstMWI GCNNNNNNNGC 3 cut(s) 406, 443, 522
BstNI CCWGG 2 cut(s) 469, 1298
BstNSI RCATGY 2 cut(s) 437, 1047
BstPI GGTNACC 1 cut(s) 546
BstSCI CCNGG 4 cut(s) 17, 467, 576, 1296
BstSFI CTRYAG 1 cut(s) 208
BstUI CGCG 2 cut(s) 632, 876
BstV1I GCAGC 3 cut(s) 424, 1031, 1241
BstXI CCANNNNNNTGG 1 cut(s) 543
Bsu15I ATCGAT 1 cut(s) 1288
BsuRI GGCC 5 cut(s) 22, 35, 66, 400, 975
BsuTUI ATCGAT 1 cut(s) 1288
BtsCI GGATG 3 cut(s) 289, 997, 1273
BtsI GCAGTG 1 cut(s) 511
BtsIMutI CAGTG 3 cut(s) 180, 217, 511
BveI ACCTGC 1 cut(s) 485
Cac8I GCNNGC 6 cut(s) 10, 328, 332, 435, 691, 1045
CciI TCATGA 1 cut(s) 1168
CfoI GCGC 2 cut(s) 14, 629
Cfr13I GGNCC 4 cut(s) 21, 33, 398, 973
ClaI ATCGAT 1 cut(s) 1288
Csp6I GTAC 3 cut(s) 168, 769, 1259
CviAII CATG 6 cut(s) 145, 434, 797, 1044, 1079, 1169
CviQI GTAC 3 cut(s) 168, 769, 1259
DdeI CTNAG 5 cut(s) 377, 593, 666, 998, 1023
DpnI GATC 3 cut(s) 112, 450, 681
DpnII GATC 3 cut(s) 110, 448, 679
DraI TTTAAA 1 cut(s) 1014
EaeI YGGCCR 1 cut(s) 64
Eam1104I CTCTTC 2 cut(s) 195, 383
EarI CTCTTC 2 cut(s) 195, 383
Eco130I CCWWGG 2 cut(s) 24, 136
Eco57I CTGAAG 2 cut(s) 156, 934
Eco88I CYCGRG 1 cut(s) 1238
Eco91I GGTNACC 1 cut(s) 546
EcoO109I RGGNCCY 1 cut(s) 398
EcoO65I GGTNACC 1 cut(s) 546
EcoRII CCWGG 2 cut(s) 467, 1296
EcoT14I CCWWGG 2 cut(s) 24, 136
EcoT22I ATGCAT 1 cut(s) 1045
ErhI CCWWGG 2 cut(s) 24, 136
Esp3I CGTCTC 1 cut(s) 189
FaeI CATG 6 cut(s) 148, 437, 800, 1047, 1082, 1172
FaqI GGGAC 2 cut(s) 611, 716
FatI CATG 6 cut(s) 144, 433, 796, 1043, 1078, 1168
FbaI TGATCA 1 cut(s) 679
FblI GTMKAC 1 cut(s) 340
Fnu4HI GCNGC 5 cut(s) 67, 438, 630, 1020, 1255
FokI GGATG 3 cut(s) 296, 1004, 1280
Fsp4HI GCNGC 5 cut(s) 67, 438, 630, 1020, 1255
FspBI CTAG 3 cut(s) 351, 405, 1146
GlaI GCGC 2 cut(s) 13, 628
GluI GCNGC 5 cut(s) 67, 438, 630, 1020, 1255
GsaI CCCAGC 1 cut(s) 34
GsuI CTGGAG 1 cut(s) 603
HaeIII GGCC 5 cut(s) 22, 35, 66, 400, 975
HapII CCGG 3 cut(s) 19, 443, 577
HhaI GCGC 2 cut(s) 14, 629
Hin1II CATG 6 cut(s) 148, 437, 800, 1047, 1082, 1172
Hin6I GCGC 2 cut(s) 12, 627
HinP1I GCGC 2 cut(s) 12, 627
HindIII AAGCTT 1 cut(s) 833
HinfI GANTC 5 cut(s) 78, 182, 924, 1131, 1160
HpaII CCGG 3 cut(s) 19, 443, 577
HphI GGTGA 1 cut(s) 540
Hpy166II GTNNAC 1 cut(s) 341
Hpy188I TCNGA 9 cut(s) 87, 187, 247, 321, 669, 759, 789, 841, 1206
Hpy188III TCNNGA 4 cut(s) 554, 620, 1146, 1169
Hpy8I GTNNAC 1 cut(s) 341
HpyAV CCTTC 8 cut(s) 266, 354, 411, 422, 744, 800, 869, 958
HpyCH4III ACNGT 2 cut(s) 212, 1263
HpyCH4V TGCA 5 cut(s) 301, 944, 1043, 1047, 1126
HpyF10VI GCNNNNNNNGC 3 cut(s) 406, 443, 522
HpyF3I CTNAG 5 cut(s) 377, 593, 666, 998, 1023
Hsp92II CATG 6 cut(s) 148, 437, 800, 1047, 1082, 1172
HspAI GCGC 2 cut(s) 12, 627
Ksp22I TGATCA 1 cut(s) 679
Kzo9I GATC 3 cut(s) 110, 448, 679
LmnI GCTCC 4 cut(s) 5, 187, 445, 622
Lsp1109I GCAGC 3 cut(s) 424, 1031, 1241
MaeI CTAG 3 cut(s) 351, 405, 1146
MaeIII GTNAC 2 cut(s) 546, 845
MalI GATC 3 cut(s) 112, 450, 681
MboI GATC 3 cut(s) 110, 448, 679
MboII GAAGA 8 cut(s) 64, 67, 70, 212, 400, 595, 685, 1241
MfeI CAATTG 1 cut(s) 939
MmeI TCCRAC 2 cut(s) 129, 1245
Mph1103I ATGCAT 1 cut(s) 1045
MroXI GAANNNNTTC 1 cut(s) 1164
MseI TTAA 5 cut(s) 417, 480, 831, 884, 1013
MspA1I CMGCKG 1 cut(s) 1022
MspI CCGG 3 cut(s) 19, 443, 577
MspR9I CCNGG 4 cut(s) 19, 469, 578, 1298
MunI CAATTG 1 cut(s) 939
Mva1269I GAATGC 4 cut(s) 303, 328, 537, 1045
MvaI CCWGG 2 cut(s) 469, 1298
MvnI CGCG 2 cut(s) 632, 876
MwoI GCNNNNNNNGC 3 cut(s) 406, 443, 522
NciI CCSGG 2 cut(s) 19, 578
NdeII GATC 3 cut(s) 110, 448, 679
NlaIII CATG 6 cut(s) 148, 437, 800, 1047, 1082, 1172
NlaIV GGNNCC 1 cut(s) 399
NmuCI GTSAC 1 cut(s) 546
NsiI ATGCAT 1 cut(s) 1045
NspI RCATGY 2 cut(s) 437, 1047
PaeI GCATGC 2 cut(s) 437, 1047
PagI TCATGA 1 cut(s) 1168
PasI CCCWGGG 1 cut(s) 468
PcsI WCGNNNNNNNCGW 1 cut(s) 251
PctI GAATGC 4 cut(s) 303, 328, 537, 1045
PdmI GAANNNNTTC 1 cut(s) 1164
PfeI GAWTC 5 cut(s) 78, 182, 924, 1131, 1160
Pfl23II CGTACG 1 cut(s) 1258
PflMI CCANNNNNTGG 1 cut(s) 30
PkrI GCNGC 5 cut(s) 68, 439, 631, 1021, 1256
Psp6I CCWGG 2 cut(s) 467, 1296
PspEI GGTNACC 1 cut(s) 546
PspFI CCCAGC 1 cut(s) 30
PspGI CCWGG 2 cut(s) 467, 1296
PspLI CGTACG 1 cut(s) 1258
PspN4I GGNNCC 1 cut(s) 399
PspPI GGNCC 4 cut(s) 21, 33, 398, 973
PvuII CAGCTG 1 cut(s) 1022
RsaI GTAC 3 cut(s) 169, 770, 1260
RsaNI GTAC 3 cut(s) 168, 769, 1259
SaqAI TTAA 5 cut(s) 417, 480, 831, 884, 1013
SatI GCNGC 5 cut(s) 67, 438, 630, 1020, 1255
Sau3AI GATC 3 cut(s) 110, 448, 679
Sau96I GGNCC 4 cut(s) 21, 33, 398, 973
ScrFI CCNGG 4 cut(s) 19, 469, 578, 1298
SfcI CTRYAG 1 cut(s) 208
SmlI CTYRAG 2 cut(s) 37, 554
SmoI CTYRAG 2 cut(s) 37, 554
SphI GCATGC 2 cut(s) 437, 1047
SsiI CCGC 6 cut(s) 67, 276, 281, 473, 630, 874
SspI AATATT 1 cut(s) 969
SspMI CTAG 3 cut(s) 351, 405, 1146
StyD4I CCNGG 4 cut(s) 17, 467, 576, 1296
StyI CCWWGG 2 cut(s) 24, 136
TaaI ACNGT 2 cut(s) 212, 1263
TaqI TCGA 2 cut(s) 500, 1288
TatI WGTACW 1 cut(s) 167
TauI GCSGC 2 cut(s) 69, 632
TfiI GAWTC 5 cut(s) 78, 182, 924, 1131, 1160
Tru1I TTAA 5 cut(s) 417, 480, 831, 884, 1013
Tru9I TTAA 5 cut(s) 417, 480, 831, 884, 1013
TscAI CASTG 3 cut(s) 180, 217, 518
TseFI GTSAC 1 cut(s) 546
TseI GCWGC 3 cut(s) 437, 1019, 1254
Tsp45I GTSAC 1 cut(s) 546
TspDTI ATGAA 9 cut(s) 827, 883, 918, 1008, 1052, 1095, 1157, 1193, 1284
TspRI CASTG 3 cut(s) 180, 217, 518
Van91I CCANNNNNTGG 1 cut(s) 30
XapI RAATTY 4 cut(s) 814, 890, 1009, 1082
XbaI TCTAGA 1 cut(s) 1145
XceI RCATGY 2 cut(s) 437, 1047
XmiI GTMKAC 1 cut(s) 340
XmnI GAANNNNTTC 1 cut(s) 1164
XspI CTAG 3 cut(s) 351, 405, 1146
Zsp2I ATGCAT 1 cut(s) 1045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.