Rh2CG596100

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0011589
Physical Location & Seq
Reverse (-)
77194033 .. 77197326
3294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG596100.1

Sequence Viewer

Length: 1011 bp
ATGCAAGGGTTGGTGGTTGTTCAGAATGCTTGCCTGCCTTGTCTACCTTCAACTAGCCCCTTGTCAGTGGATAACAAACTGAGTTTTAGGAAGAGATTGGGGCCTTCTAGGCTTTCGTTTAAGTGGAGGGAAGGGCATGCTGCTCCGGCTGATCCCACATTAGTTTCGCCCAGGGCGGTTGTTAAAAGACCAATAGCAGGTTCGACAATTCCACACTGCCCGATGGGCAAGAGAATGCCAGATTGTTGGTCACCTCTTGAGCCAAACACTTTCAAAGTCCGGGGGAGGAATTATCTTAGGGACAAAAAGAAAGAGTTAGCTCCAGATTGCGCCGCGTATTATCCTTTTGGGGTTGATATTTTTTTATCTCAGAGGAAGATTGATCATATTGCTCGCTTTGTGGAACTTCCTGTTGTGAATGTAGATGGAGATGTCCCTTCTATTCTTGTTGTAAATGTTCAGATACCTTTGTACCCTATCTCTATGTTTCAGAGCGAACATGATGGAGAAGGAATGAATTTGGTTATGTATTTTAAGCTTTCTGAAAGTTACTCAAAAGACCTTCCAGCTCATTTCCGCGAAAGTTTAACTAAATTCATCAATGATGAAGTGGAAAGAGTTAGAGGATTCCCTGTTGATACAATTGCACCCTTCAGGGAAAGATTGAAAATATTGGGCCGAATTGTAAATGTGGATGAACTCAGCAAACTGAATTTAAATGCAGCTGAGAGGAGGCTAATGAATGCATGCAATGAAAAACCTGTCCTTTCACGCCCTCAACATGAATTTTACTTGGGAGAAAACTACTTTGAGATTGATTTGGATTTGCACAGATTCAGCTATGTGTCTAGAAAGGGTATTGAATCATTTCATGAGAGAATGAAGCTATCCATACTGGATTTTGGTCTGACAATTCAGGGGAATAAGGCAGAAGAATTGCCCGAGCATATTTTATGCTGCGTACGGTTGGATGAACTTGACTACCATAATCGATACAACCTGGGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

336

Amino Acids

38.46

Weight (kDa)

8.34

Isoelectric Point (pI)

47.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 83 - 327 2.5e-55 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 188
AccI GTMKAC 1 cut(s) 43
AccII CGCG 2 cut(s) 335, 579
AciI CCGC 3 cut(s) 176, 333, 577
AclWI GGATC 1 cut(s) 146
AcsI RAATTY 4 cut(s) 517, 593, 712, 785
AcuI CTGAAG 1 cut(s) 637
AfaI GTAC 2 cut(s) 473, 963
AfiI CCNNNNNNNGG 1 cut(s) 197
AgsI TTSAA 4 cut(s) 51, 274, 667, 863
AjnI CCWGG 2 cut(s) 170, 999
AjuI GAANNNNNNNTTGG 2 cut(s) 184, 216
AluBI AGCT 6 cut(s) 320, 538, 569, 725, 840, 886
AluI AGCT 6 cut(s) 320, 538, 569, 725, 840, 886
AlwI GGATC 1 cut(s) 146
Ama87I CYCGRG 1 cut(s) 941
AoxI GGCC 2 cut(s) 101, 676
ApeKI GCWGC 3 cut(s) 140, 722, 957
ApoI RAATTY 4 cut(s) 517, 593, 712, 785
Asp700I GAANNNNTTC 1 cut(s) 867
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 2 cut(s) 101, 676
AsuC2I CCSGG 1 cut(s) 281
AsuHPI GGTGA 1 cut(s) 243
AvaI CYCGRG 1 cut(s) 941
BaeI ACNNNNGTAYC 4 cut(s) 455, 455, 488, 488
BbvI GCAGC 3 cut(s) 127, 734, 944
BccI CCATC 3 cut(s) 217, 419, 497
BciT130I CCWGG 2 cut(s) 172, 1001
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 1 cut(s) 281
BfaI CTAG 3 cut(s) 54, 108, 849
BfuAI ACCTGC 1 cut(s) 188
BglI GCCNNNNNGGC 2 cut(s) 109, 225
BisI GCNGC 4 cut(s) 141, 333, 723, 958
BlsI GCNGC 4 cut(s) 142, 334, 724, 959
Bme1390I CCNGG 3 cut(s) 172, 281, 1001
BmeT110I CYCGRG 1 cut(s) 941
BmgT120I GGNCC 2 cut(s) 101, 676
BmiI GGNNCC 1 cut(s) 102
BmrFI CCNGG 3 cut(s) 172, 281, 1001
BpmI CTGGAG 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 278
BpuMI CCSGG 1 cut(s) 281
Bsa29I ATCGAT 1 cut(s) 991
BsaJI CCNNGG 4 cut(s) 170, 171, 280, 1000
Bsc4I CCNNNNNNNGG 1 cut(s) 197
Bse1I ACTGG 1 cut(s) 900
Bse3DI GCAATG 1 cut(s) 757
BseBI CCWGG 2 cut(s) 172, 1001
BseCI ATCGAT 1 cut(s) 991
BseDI CCNNGG 4 cut(s) 170, 171, 280, 1000
BseGI GGATG 2 cut(s) 700, 976
BseLI CCNNNNNNNGG 1 cut(s) 197
BseMI GCAATG 1 cut(s) 757
BseMII CTCAG 4 cut(s) 71, 383, 715, 717
BseNI ACTGG 1 cut(s) 900
BseRI GAGGAG 1 cut(s) 745
BseXI GCAGC 3 cut(s) 127, 734, 944
Bsh1236I CGCG 2 cut(s) 335, 579
BshFI GGCC 2 cut(s) 103, 678
BshVI ATCGAT 1 cut(s) 991
BsiHKCI CYCGRG 1 cut(s) 941
BsiSI CCGG 2 cut(s) 146, 280
BsiWI CGTACG 1 cut(s) 961
BslFI GGGAC 2 cut(s) 314, 419
BslI CCNNNNNNNGG 1 cut(s) 197
BsmFI GGGAC 2 cut(s) 314, 419
BsmI GAATGC 3 cut(s) 31, 240, 748
BsnI GGCC 2 cut(s) 103, 678
BsoBI CYCGRG 1 cut(s) 941
Bsp143I GATC 2 cut(s) 151, 382
BspACI CCGC 3 cut(s) 176, 333, 577
BspANI GGCC 2 cut(s) 103, 678
BspCNI CTCAG 4 cut(s) 72, 382, 714, 718
BspDI ATCGAT 1 cut(s) 991
BspFNI CGCG 2 cut(s) 335, 579
BspHI TCATGA 1 cut(s) 871
BspLI GGNNCC 1 cut(s) 102
BspMI ACCTGC 1 cut(s) 188
BspPI GGATC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 757
BsrI ACTGG 1 cut(s) 900
BssECI CCNNGG 4 cut(s) 170, 171, 280, 1000
BssMI GATC 2 cut(s) 151, 382
Bst2UI CCWGG 2 cut(s) 172, 1001
Bst4CI ACNGT 1 cut(s) 966
Bst6I CTCTTC 1 cut(s) 86
BstC8I GCNNGC 5 cut(s) 31, 35, 138, 394, 748
BstDEI CTNAG 5 cut(s) 80, 296, 369, 701, 726
BstEII GGTNACC 1 cut(s) 249
BstF5I GGATG 2 cut(s) 700, 976
BstFNI CGCG 2 cut(s) 335, 579
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 2 cut(s) 154, 385
BstMBI GATC 2 cut(s) 151, 382
BstMWI GCNNNNNNNGC 3 cut(s) 109, 146, 225
BstNI CCWGG 2 cut(s) 172, 1001
BstNSI RCATGY 2 cut(s) 140, 750
BstPI GGTNACC 1 cut(s) 249
BstSCI CCNGG 3 cut(s) 170, 279, 999
BstUI CGCG 2 cut(s) 335, 579
BstV1I GCAGC 3 cut(s) 127, 734, 944
BstXI CCANNNNNNTGG 1 cut(s) 246
Bsu15I ATCGAT 1 cut(s) 991
BsuRI GGCC 2 cut(s) 103, 678
BsuTUI ATCGAT 1 cut(s) 991
BtsCI GGATG 2 cut(s) 700, 976
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 2 cut(s) 72, 214
BveI ACCTGC 1 cut(s) 188
Cac8I GCNNGC 5 cut(s) 31, 35, 138, 394, 748
CciI TCATGA 1 cut(s) 871
CfoI GCGC 1 cut(s) 332
Cfr13I GGNCC 2 cut(s) 101, 676
ClaI ATCGAT 1 cut(s) 991
Csp6I GTAC 2 cut(s) 472, 962
CviAII CATG 5 cut(s) 137, 500, 747, 782, 872
CviQI GTAC 2 cut(s) 472, 962
DdeI CTNAG 5 cut(s) 80, 296, 369, 701, 726
DpnI GATC 2 cut(s) 153, 384
DpnII GATC 2 cut(s) 151, 382
DraI TTTAAA 1 cut(s) 717
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
Eco57I CTGAAG 1 cut(s) 637
Eco88I CYCGRG 1 cut(s) 941
Eco91I GGTNACC 1 cut(s) 249
EcoO109I RGGNCCY 1 cut(s) 101
EcoO65I GGTNACC 1 cut(s) 249
EcoRII CCWGG 2 cut(s) 170, 999
EcoT22I ATGCAT 1 cut(s) 748
FaeI CATG 5 cut(s) 140, 503, 750, 785, 875
FaqI GGGAC 2 cut(s) 314, 419
FatI CATG 5 cut(s) 136, 499, 746, 781, 871
FbaI TGATCA 1 cut(s) 382
FblI GTMKAC 1 cut(s) 43
Fnu4HI GCNGC 4 cut(s) 141, 333, 723, 958
FokI GGATG 2 cut(s) 707, 983
Fsp4HI GCNGC 4 cut(s) 141, 333, 723, 958
FspBI CTAG 3 cut(s) 54, 108, 849
GlaI GCGC 1 cut(s) 331
GluI GCNGC 4 cut(s) 141, 333, 723, 958
GsuI CTGGAG 1 cut(s) 306
HaeIII GGCC 2 cut(s) 103, 678
HapII CCGG 2 cut(s) 146, 280
HhaI GCGC 1 cut(s) 332
Hin1II CATG 5 cut(s) 140, 503, 750, 785, 875
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 3 cut(s) 627, 834, 863
HpaII CCGG 2 cut(s) 146, 280
HphI GGTGA 1 cut(s) 243
Hpy166II GTNNAC 1 cut(s) 44
Hpy188I TCNGA 6 cut(s) 24, 372, 462, 492, 544, 909
Hpy188III TCNNGA 4 cut(s) 257, 323, 849, 872
Hpy8I GTNNAC 1 cut(s) 44
HpyAV CCTTC 7 cut(s) 57, 114, 125, 447, 503, 572, 661
HpyCH4III ACNGT 1 cut(s) 966
HpyCH4V TGCA 6 cut(s) 4, 647, 722, 746, 750, 829
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 146, 225
HpyF3I CTNAG 5 cut(s) 80, 296, 369, 701, 726
Hsp92II CATG 5 cut(s) 140, 503, 750, 785, 875
HspAI GCGC 1 cut(s) 330
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 2 cut(s) 151, 382
LmnI GCTCC 2 cut(s) 148, 325
Lsp1109I GCAGC 3 cut(s) 127, 734, 944
MaeI CTAG 3 cut(s) 54, 108, 849
MaeIII GTNAC 2 cut(s) 249, 548
MalI GATC 2 cut(s) 153, 384
MboI GATC 2 cut(s) 151, 382
MboII GAAGA 3 cut(s) 103, 388, 944
MfeI CAATTG 1 cut(s) 642
MmeI TCCRAC 1 cut(s) 948
MnlI CCTC 8 cut(s) 120, 264, 279, 366, 617, 723, 726, 786
Mph1103I ATGCAT 1 cut(s) 748
MroXI GAANNNNTTC 1 cut(s) 867
MseI TTAA 5 cut(s) 120, 183, 534, 587, 716
MspA1I CMGCKG 1 cut(s) 725
MspI CCGG 2 cut(s) 146, 280
MspR9I CCNGG 3 cut(s) 172, 281, 1001
MunI CAATTG 1 cut(s) 642
Mva1269I GAATGC 3 cut(s) 31, 240, 748
MvaI CCWGG 2 cut(s) 172, 1001
MvnI CGCG 2 cut(s) 335, 579
MwoI GCNNNNNNNGC 3 cut(s) 109, 146, 225
NciI CCSGG 1 cut(s) 281
NdeII GATC 2 cut(s) 151, 382
NlaIII CATG 5 cut(s) 140, 503, 750, 785, 875
NlaIV GGNNCC 1 cut(s) 102
NmuCI GTSAC 1 cut(s) 249
NsiI ATGCAT 1 cut(s) 748
NspI RCATGY 2 cut(s) 140, 750
PaeI GCATGC 2 cut(s) 140, 750
PagI TCATGA 1 cut(s) 871
PasI CCCWGGG 1 cut(s) 171
PctI GAATGC 3 cut(s) 31, 240, 748
PdmI GAANNNNTTC 1 cut(s) 867
PfeI GAWTC 3 cut(s) 627, 834, 863
Pfl23II CGTACG 1 cut(s) 961
PkrI GCNGC 4 cut(s) 142, 334, 724, 959
Psp6I CCWGG 2 cut(s) 170, 999
PspEI GGTNACC 1 cut(s) 249
PspGI CCWGG 2 cut(s) 170, 999
PspLI CGTACG 1 cut(s) 961
PspN4I GGNNCC 1 cut(s) 102
PspPI GGNCC 2 cut(s) 101, 676
PvuII CAGCTG 1 cut(s) 725
RsaI GTAC 2 cut(s) 473, 963
RsaNI GTAC 2 cut(s) 472, 962
SaqAI TTAA 5 cut(s) 120, 183, 534, 587, 716
SatI GCNGC 4 cut(s) 141, 333, 723, 958
Sau3AI GATC 2 cut(s) 151, 382
Sau96I GGNCC 2 cut(s) 101, 676
ScrFI CCNGG 3 cut(s) 172, 281, 1001
SmiI ATTTAAAT 1 cut(s) 717
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
SphI GCATGC 2 cut(s) 140, 750
SsiI CCGC 3 cut(s) 176, 333, 577
SspI AATATT 1 cut(s) 672
SspMI CTAG 3 cut(s) 54, 108, 849
StyD4I CCNGG 3 cut(s) 170, 279, 999
SwaI ATTTAAAT 1 cut(s) 717
TaaI ACNGT 1 cut(s) 966
TaqI TCGA 2 cut(s) 203, 991
TauI GCSGC 1 cut(s) 335
TfiI GAWTC 3 cut(s) 627, 834, 863
Tru1I TTAA 5 cut(s) 120, 183, 534, 587, 716
Tru9I TTAA 5 cut(s) 120, 183, 534, 587, 716
TscAI CASTG 2 cut(s) 72, 221
TseFI GTSAC 1 cut(s) 249
TseI GCWGC 3 cut(s) 140, 722, 957
Tsp45I GTSAC 1 cut(s) 249
TspRI CASTG 2 cut(s) 72, 221
XapI RAATTY 4 cut(s) 517, 593, 712, 785
XbaI TCTAGA 1 cut(s) 848
XceI RCATGY 2 cut(s) 140, 750
XmiI GTMKAC 1 cut(s) 43
XmnI GAANNNNTTC 1 cut(s) 867
XspI CTAG 3 cut(s) 54, 108, 849
Zsp2I ATGCAT 1 cut(s) 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.