MD17G1044300.v1.1

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011589
Physical Location & Seq
Forward (+)
3224753 .. 3227752
3000 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1044300.v1.1.491

Sequence Viewer

Length: 1422 bp
ATGGGAGCTTGTGCTTCGAGGCCGAAGGGCTGCAGTTTCGGATTCTCGAAGAAGAAAAAGAAGAATGGCGGCGGCCGCCGCCTGAGACGAGTCATTCGCCGGCGCGTTTCGTCGTCCTCCGGTCGCTCCGTTCCTGCACTCCAAGGAAGTATGGATGGGGCGTTTTTTGATGCAAATTCGGCGATCGAGTCGGAGCGAGATGACGATTTTTACAGTGTTTACGATGATGTGATGTCATTAAACGAGTCGGAAAGTGCATCCACGTTGAGTGTTTTGTCACCGAGAGGTCTTTCTCGGCAGGCACAGAAACCGCCGCCATTGCATTCCACCTCCGCTGTGTCTGCAGAGGAGATCGTGGACGAACATGGCGGAGGGGACAGAATGCAGGGACTGGATCACTGTGGGATTCTTCAAAATTCTTGCTTGCCTTGTCGTCCTTCCAATAGCCTGTCGGGCGACAAAAGAATAACCACGGCCACGCCAAGTTTGAGGAGGAAAGGCCTTTCGTTTAAATGGAGGGAAGGGCATTCTGCTGCAGCTGCAGTTGATCACACCCCCACATTACTTTCACCGAGATCGCTTGCGAAAAGACCACTAGCCGGTTCTACAATTCCCTACTGTCCCATTGAGAAGAGAATGCCAGATTGTTGGTCACCTCTCGAGCCAAACACTTTCAAAGTCCGGGGTAAAAATTATCTTAGGGATAAGAAGAAAGAAATTGCTCCGAATTGCGCTGCATTTTATCCTTTTGCCGCTGATATTTTCGTATCTCCGAAAAAGATTGATCACATTGCTCGATTTGTGAAACTTCCGGTTGTGAGTACCACCGGAGATGTCCCTTCTATTCTTGTTGTAAATGTTCAGATACCACTTTATCCAGCCAATTTTTTCATAGGCGATGGTGATGGAGAAGGAATGAACTTGGTTATGTATTGTAAGATTTCTGAAACTTATTCGAAGGAGCTTCCTCCTCATTTCCGAGAAAGTATCACTAGATTAATCAATGATGAAGTGGAGAGAGTTAGAGGATTCCCTGTCGATACAATTGCACCCTTCAGGGAAAGATTGAAAATTTTGGGCCGAGTGGCAAATTTGGAGGATCTTCATTTAAACACAGCCGAGAAGAAGCTAATGAATGCTTACAATGAAAAACCTGTTCTTTCACGTCCTCAACATGAATTTTACTTGGGAGAAAACTACTTCGAGATCGATTTGGATATGCACAGATTCAGCTACATCCCTAGAAAAGGTATTGATGCATTTCACGAAAGAATGAAGCTGACGATATTGGATTTTGGTCTCACAATTCAGGGGAACAAAGCAGAAGAGTTGCCGGAGCATTTGTTATGCTGCATACGGTTCAACAAAGTTGACTACAATAATAAATCATTACCGGTGGTTCTGAATCAAAAGTTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

474

Amino Acids

52.95

Weight (kDa)

9.15

Isoelectric Point (pI)

53.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 217 - 458 5e-55 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 105
AciI CCGC 9 cut(s) 69, 72, 76, 79, 311, 314, 333, 369, 753
AclWI GGATC 2 cut(s) 402, 1107
AcoI YGGCCR 2 cut(s) 73, 474
AcsI RAATTY 5 cut(s) 175, 415, 1071, 1090, 1178
AcuI CTGAAG 1 cut(s) 1039
AfaI GTAC 1 cut(s) 823
AfiI CCNNNNNNNGG 2 cut(s) 599, 1247
AgeI ACCGGT 1 cut(s) 1393
AgsI TTSAA 4 cut(s) 413, 676, 1069, 1363
AjiI CACGTC 1 cut(s) 1166
AluBI AGCT 6 cut(s) 8, 539, 964, 1129, 1233, 1279
AluI AGCT 6 cut(s) 8, 539, 964, 1129, 1233, 1279
Alw26I GTCTC 2 cut(s) 79, 1304
AlwI GGATC 2 cut(s) 402, 1107
AlwNI CAGNNNCTG 1 cut(s) 391
Ama87I CYCGRG 1 cut(s) 659
AoxI GGCC 5 cut(s) 20, 73, 474, 499, 1078
ApeKI GCWGC 6 cut(s) 30, 533, 536, 539, 734, 1350
ApoI RAATTY 5 cut(s) 175, 415, 1071, 1090, 1178
ArsI GACNNNNNNTTYG 2 cut(s) 78, 110
AseI ATTAAT 1 cut(s) 998
AsiGI ACCGGT 1 cut(s) 1393
AspLEI GCGC 2 cut(s) 105, 734
AspS9I GGNCC 1 cut(s) 1078
AsuC2I CCSGG 1 cut(s) 683
AsuHPI GGTGA 4 cut(s) 270, 561, 645, 914
AsuII TTCGAA 1 cut(s) 956
AvaI CYCGRG 1 cut(s) 659
BbvI GCAGC 6 cut(s) 17, 520, 526, 548, 721, 1337
BccI CCATC 3 cut(s) 149, 893, 899
BceAI ACGGC 1 cut(s) 489
BcgI CGANNNNNNTGC 2 cut(s) 1028, 1062
BclI TGATCA 2 cut(s) 547, 784
BcnI CCSGG 1 cut(s) 683
BcoDI GTCTC 2 cut(s) 79, 1304
BfaI CTAG 3 cut(s) 596, 993, 1242
BfmI CTRYAG 4 cut(s) 31, 342, 534, 540
BglI GCCNNNNNGGC 1 cut(s) 453
Bme1390I CCNGG 1 cut(s) 683
BmeT110I CYCGRG 1 cut(s) 659
BmgBI CACGTC 1 cut(s) 1166
BmgT120I GGNCC 1 cut(s) 1078
BmrFI CCNGG 1 cut(s) 683
BmsI GCATC 3 cut(s) 160, 266, 1246
Bpu14I TTCGAA 1 cut(s) 956
BpuMI CCSGG 1 cut(s) 683
Bsa29I ATCGAT 1 cut(s) 1209
BsaI GGTCTC 1 cut(s) 1304
BsaJI CCNNGG 3 cut(s) 142, 471, 682
BsaWI WCCGGW 4 cut(s) 119, 811, 827, 1393
BsaXI ACNNNNNCTCC 2 cut(s) 1328, 1358
Bsc4I CCNNNNNNNGG 2 cut(s) 599, 1247
Bse118I RCCGGY 3 cut(s) 99, 599, 1393
Bse1I ACTGG 1 cut(s) 396
Bse3DI GCAATG 2 cut(s) 317, 789
BseCI ATCGAT 1 cut(s) 1209
BseDI CCNNGG 3 cut(s) 142, 471, 682
BseGI GGATG 3 cut(s) 160, 257, 1236
BseLI CCNNNNNNNGG 2 cut(s) 599, 1247
BseMI GCAATG 2 cut(s) 317, 789
BseMII CTCAG 1 cut(s) 74
BseNI ACTGG 1 cut(s) 396
BseRI GAGGAG 3 cut(s) 362, 505, 960
BseX3I CGGCCG 1 cut(s) 73
BseXI GCAGC 6 cut(s) 17, 520, 526, 548, 721, 1337
BsgI GTGCAG 1 cut(s) 120
Bsh1236I CGCG 1 cut(s) 105
Bsh1285I CGRYCG 3 cut(s) 76, 124, 186
BshFI GGCC 5 cut(s) 22, 75, 476, 501, 1080
BshTI ACCGGT 1 cut(s) 1393
BshVI ATCGAT 1 cut(s) 1209
BsiEI CGRYCG 3 cut(s) 76, 124, 186
BsiHKCI CYCGRG 1 cut(s) 659
BsiSI CCGG 8 cut(s) 100, 120, 600, 682, 812, 828, 1334, 1394
BslFI GGGAC 4 cut(s) 389, 402, 606, 821
BslI CCNNNNNNNGG 2 cut(s) 599, 1247
BsmAI GTCTC 2 cut(s) 79, 1304
BsmBI CGTCTC 1 cut(s) 79
BsmFI GGGAC 4 cut(s) 389, 402, 606, 821
BsmI GAATGC 5 cut(s) 322, 387, 526, 642, 1141
BsnI GGCC 5 cut(s) 22, 75, 476, 501, 1080
Bso31I GGTCTC 1 cut(s) 1304
BsoBI CYCGRG 1 cut(s) 659
Bsp119I TTCGAA 1 cut(s) 956
Bsp143I GATC 8 cut(s) 183, 351, 394, 547, 575, 784, 1099, 1206
BspACI CCGC 9 cut(s) 69, 72, 76, 79, 311, 314, 333, 369, 753
BspANI GGCC 5 cut(s) 22, 75, 476, 501, 1080
BspCNI CTCAG 1 cut(s) 75
BspDI ATCGAT 1 cut(s) 1209
BspFNI CGCG 1 cut(s) 105
BspMAI CTGCAG 4 cut(s) 35, 346, 538, 544
BspPI GGATC 2 cut(s) 402, 1107
BspT104I TTCGAA 1 cut(s) 956
BspTNI GGTCTC 1 cut(s) 1304
BsrDI GCAATG 2 cut(s) 317, 789
BsrFI RCCGGY 3 cut(s) 99, 599, 1393
BsrI ACTGG 1 cut(s) 396
BssAI RCCGGY 3 cut(s) 99, 599, 1393
BssECI CCNNGG 3 cut(s) 142, 471, 682
BssMI GATC 8 cut(s) 183, 351, 394, 547, 575, 784, 1099, 1206
BssT1I CCWWGG 1 cut(s) 142
Bst4CI ACNGT 4 cut(s) 215, 401, 620, 1359
Bst6I CTCTTC 2 cut(s) 626, 1320
BstBI TTCGAA 1 cut(s) 956
BstC8I GCNNGC 4 cut(s) 101, 300, 425, 582
BstDEI CTNAG 2 cut(s) 83, 698
BstDSI CCRYGG 1 cut(s) 471
BstEII GGTNACC 1 cut(s) 651
BstENI CCTNNNNNAGG 1 cut(s) 1245
BstF5I GGATG 3 cut(s) 160, 257, 1236
BstFNI CGCG 1 cut(s) 105
BstHHI GCGC 2 cut(s) 105, 734
BstKTI GATC 8 cut(s) 186, 354, 397, 550, 578, 787, 1102, 1209
BstMAI GTCTC 2 cut(s) 79, 1304
BstMBI GATC 8 cut(s) 183, 351, 394, 547, 575, 784, 1099, 1206
BstMCI CGRYCG 3 cut(s) 76, 124, 186
BstMWI GCNNNNNNNGC 7 cut(s) 75, 78, 179, 319, 341, 453, 539
BstPI GGTNACC 1 cut(s) 651
BstSCI CCNGG 1 cut(s) 681
BstSFI CTRYAG 4 cut(s) 31, 342, 534, 540
BstUI CGCG 1 cut(s) 105
BstV1I GCAGC 6 cut(s) 17, 520, 526, 548, 721, 1337
BstX2I RGATCY 1 cut(s) 1099
BstXI CCANNNNNNTGG 1 cut(s) 648
BstYI RGATCY 1 cut(s) 1099
BstZI CGGCCG 1 cut(s) 73
Bsu15I ATCGAT 1 cut(s) 1209
BsuRI GGCC 5 cut(s) 22, 75, 476, 501, 1080
BsuTUI ATCGAT 1 cut(s) 1209
BtgI CCRYGG 1 cut(s) 471
BtgZI GCGATG 1 cut(s) 912
BtrI CACGTC 1 cut(s) 1166
BtsCI GGATG 3 cut(s) 160, 257, 1236
BtsIMutI CAGTG 2 cut(s) 220, 397
Cac8I GCNNGC 4 cut(s) 101, 300, 425, 582
CaiI CAGNNNCTG 1 cut(s) 391
CciNI GCGGCCGC 1 cut(s) 73
CfoI GCGC 2 cut(s) 105, 734
Cfr10I RCCGGY 3 cut(s) 99, 599, 1393
Cfr13I GGNCC 1 cut(s) 1078
ClaI ATCGAT 1 cut(s) 1209
Csp6I GTAC 1 cut(s) 822
CspAI ACCGGT 1 cut(s) 1393
CviAII CATG 2 cut(s) 365, 1175
CviQI GTAC 1 cut(s) 822
DdeI CTNAG 2 cut(s) 83, 698
DpnI GATC 8 cut(s) 185, 353, 396, 549, 577, 786, 1101, 1208
DpnII GATC 8 cut(s) 183, 351, 394, 547, 575, 784, 1099, 1206
DraI TTTAAA 2 cut(s) 511, 1110
EaeI YGGCCR 2 cut(s) 73, 474
EagI CGGCCG 1 cut(s) 73
Eam1104I CTCTTC 2 cut(s) 626, 1320
EarI CTCTTC 2 cut(s) 626, 1320
EciI GGCGGA 1 cut(s) 384
EclXI CGGCCG 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 142
Eco147I AGGCCT 1 cut(s) 501
Eco31I GGTCTC 1 cut(s) 1304
Eco52I CGGCCG 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 1039
Eco88I CYCGRG 1 cut(s) 659
Eco91I GGTNACC 1 cut(s) 651
EcoNI CCTNNNNNAGG 1 cut(s) 1245
EcoO65I GGTNACC 1 cut(s) 651
EcoT14I CCWWGG 1 cut(s) 142
EcoT22I ATGCAT 1 cut(s) 1261
ErhI CCWWGG 1 cut(s) 142
Esp3I CGTCTC 1 cut(s) 79
FaeI CATG 2 cut(s) 368, 1178
FaiI YATR 8 cut(s) 152, 366, 893, 929, 1176, 1220, 1348, 1355
FaqI GGGAC 4 cut(s) 389, 402, 606, 821
FatI CATG 2 cut(s) 364, 1174
FbaI TGATCA 2 cut(s) 547, 784
FokI GGATG 3 cut(s) 167, 244, 1223
FspBI CTAG 3 cut(s) 596, 993, 1242
GlaI GCGC 2 cut(s) 104, 733
HaeIII GGCC 5 cut(s) 22, 75, 476, 501, 1080
HapII CCGG 8 cut(s) 100, 120, 600, 682, 812, 828, 1334, 1394
HhaI GCGC 2 cut(s) 105, 734
Hin1II CATG 2 cut(s) 368, 1178
Hin6I GCGC 2 cut(s) 103, 732
HinP1I GCGC 2 cut(s) 103, 732
HincII GTYRAC 1 cut(s) 1372
HindII GTYRAC 1 cut(s) 1372
HinfI GANTC 8 cut(s) 42, 90, 188, 245, 406, 1029, 1227, 1405
HpaII CCGG 8 cut(s) 100, 120, 600, 682, 812, 828, 1334, 1394
HphI GGTGA 4 cut(s) 270, 561, 645, 914
Hpy166II GTNNAC 3 cut(s) 220, 358, 1372
Hpy188I TCNGA 9 cut(s) 41, 193, 250, 726, 774, 864, 946, 980, 1404
Hpy188III TCNNGA 4 cut(s) 46, 659, 1204, 1265
Hpy8I GTNNAC 3 cut(s) 220, 358, 1372
Hpy99I CGWCG 1 cut(s) 115
HpyAV CCTTC 7 cut(s) 19, 447, 515, 849, 905, 952, 1063
HpyCH4III ACNGT 4 cut(s) 215, 401, 620, 1359
HpyCH4IV ACGT 2 cut(s) 263, 1165
HpyF10VI GCNNNNNNNGC 7 cut(s) 75, 78, 179, 319, 341, 453, 539
HpyF3I CTNAG 2 cut(s) 83, 698
HpySE526I ACGT 2 cut(s) 263, 1165
Hsp92II CATG 2 cut(s) 368, 1178
HspAI GCGC 2 cut(s) 103, 732
KroI GCCGGC 1 cut(s) 99
KroNI GCCGGC 1 cut(s) 101
Ksp22I TGATCA 2 cut(s) 547, 784
Kzo9I GATC 8 cut(s) 183, 351, 394, 547, 575, 784, 1099, 1206
LmnI GCTCC 6 cut(s) 5, 131, 193, 727, 961, 1336
Lsp1109I GCAGC 6 cut(s) 17, 520, 526, 548, 721, 1337
LweI GCATC 3 cut(s) 160, 266, 1246
MaeI CTAG 3 cut(s) 596, 993, 1242
MaeII ACGT 2 cut(s) 263, 1165
MaeIII GTNAC 2 cut(s) 276, 651
MalI GATC 8 cut(s) 185, 353, 396, 549, 577, 786, 1101, 1208
MboI GATC 8 cut(s) 183, 351, 394, 547, 575, 784, 1099, 1206
MboII GAAGA 9 cut(s) 61, 64, 73, 401, 643, 721, 1094, 1135, 1337
MfeI CAATTG 1 cut(s) 1044
MflI RGATCY 1 cut(s) 1099
MlyI GAGTC 3 cut(s) 99, 197, 254
MmeI TCCRAC 2 cut(s) 171, 228
Mph1103I ATGCAT 1 cut(s) 1261
MreI CGCCGGCG 1 cut(s) 99
MroNI GCCGGC 1 cut(s) 99
MseI TTAA 4 cut(s) 239, 510, 998, 1109
MspA1I CMGCKG 3 cut(s) 335, 539, 755
MspI CCGG 8 cut(s) 100, 120, 600, 682, 812, 828, 1334, 1394
MspR9I CCNGG 1 cut(s) 683
MunI CAATTG 1 cut(s) 1044
Mva1269I GAATGC 5 cut(s) 322, 387, 526, 642, 1141
MvnI CGCG 1 cut(s) 105
MwoI GCNNNNNNNGC 7 cut(s) 75, 78, 179, 319, 341, 453, 539
NaeI GCCGGC 1 cut(s) 101
NciI CCSGG 1 cut(s) 683
NdeII GATC 8 cut(s) 183, 351, 394, 547, 575, 784, 1099, 1206
NgoMIV GCCGGC 1 cut(s) 99
NlaIII CATG 2 cut(s) 368, 1178
NmeAIII GCCGAG 3 cut(s) 274, 1106, 1144
NmuCI GTSAC 2 cut(s) 276, 651
NotI GCGGCCGC 1 cut(s) 73
NsiI ATGCAT 1 cut(s) 1261
NspV TTCGAA 1 cut(s) 956
PaeR7I CTCGAG 1 cut(s) 659
PceI AGGCCT 1 cut(s) 501
PctI GAATGC 5 cut(s) 322, 387, 526, 642, 1141
PdiI GCCGGC 1 cut(s) 101
PfeI GAWTC 5 cut(s) 42, 406, 1029, 1227, 1405
PinAI ACCGGT 1 cut(s) 1393
Ple19I CGATCG 1 cut(s) 186
PleI GAGTC 3 cut(s) 98, 196, 253
PpsI GAGTC 3 cut(s) 98, 196, 253
PshBI ATTAAT 1 cut(s) 998
PspEI GGTNACC 1 cut(s) 651
PspPI GGNCC 1 cut(s) 1078
PstI CTGCAG 4 cut(s) 35, 346, 538, 544
PstNI CAGNNNCTG 1 cut(s) 391
PsuI RGATCY 1 cut(s) 1099
PvuI CGATCG 1 cut(s) 186
PvuII CAGCTG 1 cut(s) 539
RsaI GTAC 1 cut(s) 823
RsaNI GTAC 1 cut(s) 822
SaqAI TTAA 4 cut(s) 239, 510, 998, 1109
Sau3AI GATC 8 cut(s) 183, 351, 394, 547, 575, 784, 1099, 1206
Sau96I GGNCC 1 cut(s) 1078
SchI GAGTC 3 cut(s) 99, 197, 254
ScrFI CCNGG 1 cut(s) 683
SfaNI GCATC 3 cut(s) 160, 266, 1246
SfcI CTRYAG 4 cut(s) 31, 342, 534, 540
Sfr274I CTCGAG 1 cut(s) 659
SfuI TTCGAA 1 cut(s) 956
SgrAI CRCCGGYG 1 cut(s) 99
SlaI CTCGAG 1 cut(s) 659
SmlI CTYRAG 1 cut(s) 659
SmoI CTYRAG 1 cut(s) 659
SseBI AGGCCT 1 cut(s) 501
SsiI CCGC 9 cut(s) 69, 72, 76, 79, 311, 314, 333, 369, 753
SspMI CTAG 3 cut(s) 596, 993, 1242
StuI AGGCCT 1 cut(s) 501
StyD4I CCNGG 1 cut(s) 681
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 4 cut(s) 215, 401, 620, 1359
TaiI ACGT 2 cut(s) 266, 1168
TaqI TCGA 9 cut(s) 17, 47, 186, 660, 796, 956, 1038, 1203, 1209
TauI GCSGC 6 cut(s) 72, 75, 78, 81, 316, 755
TfiI GAWTC 5 cut(s) 42, 406, 1029, 1227, 1405
Tru1I TTAA 4 cut(s) 239, 510, 998, 1109
Tru9I TTAA 4 cut(s) 239, 510, 998, 1109
TscAI CASTG 2 cut(s) 220, 404
TseFI GTSAC 2 cut(s) 276, 651
TseI GCWGC 6 cut(s) 30, 533, 536, 539, 734, 1350
Tsp45I GTSAC 2 cut(s) 276, 651
TspDTI ATGAA 8 cut(s) 880, 932, 1023, 1094, 1148, 1161, 1191, 1289
TspGWI ACGGA 1 cut(s) 118
TspRI CASTG 2 cut(s) 220, 404
VspI ATTAAT 1 cut(s) 998
XagI CCTNNNNNAGG 1 cut(s) 1245
XapI RAATTY 5 cut(s) 175, 415, 1071, 1090, 1178
XhoI CTCGAG 1 cut(s) 659
XspI CTAG 3 cut(s) 596, 993, 1242
Zsp2I ATGCAT 1 cut(s) 1261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.