MD09G1042800.v1.1

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011589
Physical Location & Seq
Forward (+)
2761557 .. 2765020
3464 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1042800.v1.1.491

Sequence Viewer

Length: 1440 bp
ATGGGGGCTTGTGCTTCGAGGCCGAAGGGCTGCAGTTTCGGATTCAAGAAGAAGAAGAAGAATGACGGCGGCGCCCGCCGCCGGAGGCGAGTCATTCCCCGGCGCGTTTCGTCGGCCTCCGATCGCTCTCACTCCGTTCCTACACACCAAGGAAGTATGGATGCATCGTTTTTTGATGCGAATTCGGCGATCGAGTCGGAGCGAGATGACGAATTTTACAGTGTTCATGATGATGTGATGTCATTACACGAGTCGGAAAGTGCATCCACATTGAGTGTGTCTTCACCGAGAGGTCTTTCTCGGCTGGCACTGAAACCGCCGCCATTGCATTCCACCTCCGCTGTGTCCGCGGAGGTGACTGTGGATGAAATTGGCGGAGGGGACAAGATGCAGGGACTGGATCATTGTTGGATTCTTCAAACTTCTTGCTTGCCTTGTCTTCCTTCCACTTCCAATAGCCCGTTGGGCGACAAAAGAATAACCCCGGCCGCGCCAAGCTTGAGGAGGAAAGTCCTTTCCTTTAAATGGAGGGAAGGGCATTCTGCTAGTGCTGCAGCTGCAGCTGATTCCACCCCCACATTACTTTCCCCCAGAACGCTTGCGAAAAGACCACTTGCCGGTTCTACAATTCCGTACTGTCCCGTTGAGAAGAGAATTCCAGATTGTTGGTCACCTCTCGAGCCAAATACATTCAAAGTCCGGGGTAAAAATTATCTTAGGGATAAAAAGAAAGAAATTGCTCCGAGCTGCGCTGCATTTTATCCTTTCGCCGCTGATATTTTCGTATCTCAGAAAAAGATTGATCACATTGCTCGTTTTGTGGAACTTCCAGTTGTGAGTACCACTGGAGACATCCCGTCTATTCTTGTTGTAAATGTTCAGATACCACTTTATCCAGCCAATTTTTTTCAAGGCGATAGTGATGGAGAAGGAATGAACTTGGTTATGTACTATAAGATTTCTGAAACTTATTCCAAAGAGCTTCCCCCTCATTTCCGAGAAAGTATCACTAGACTGATCAATGACGAAGTGGAGAGAGTTAGAGGATTCCCTGTCGATACAATTGCACCCTTCAGGGAAAGATTGAAAATTTTGGGCCGAGTGGCAAATTTGGAGGATCTTCATTTAAGCACAGCCGAGAAGAAGCTAATGAATGCTTACAATGAAAAACCTGTTCTTTCACGTCCTCAACATGAATTTTACTTGGGAGAAAACTACTTCGAGATCGATTTGGATATGCACAGATTCAGCTACATCTCTAGAAAAGGTATTGAAGCATTTCACGAAAGAATGAAGCTGTCGGTATTGGATTTTGGTCTCACAATTCAGGGGAACAAGCCAGAAGACTTGCCTGAACATTTGTTATGCAGCATACGGTTGAACAAAGTTGACTACAATAATAAATCATTACCAGGAGTTTTGAATCCAAAGTCTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

480

Amino Acids

53.43

Weight (kDa)

8.96

Isoelectric Point (pI)

53.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 223 - 464 9.6e-55 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 71
AccII CGCG 3 cut(s) 105, 350, 491
AclWI GGATC 2 cut(s) 408, 1125
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 6 cut(s) 181, 212, 654, 1089, 1108, 1196
AcuI CTGAAG 1 cut(s) 1057
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 3 cut(s) 635, 841, 950
AfiI CCNNNNNNNGG 3 cut(s) 81, 525, 617
AgsI TTSAA 8 cut(s) 46, 419, 694, 911, 1087, 1274, 1381, 1423
AhdI GACNNNNNGTC 1 cut(s) 856
AjiI CACGTC 1 cut(s) 1184
AjnI CCWGG 1 cut(s) 1411
AluBI AGCT 8 cut(s) 498, 557, 563, 747, 982, 1147, 1251, 1297
AluI AGCT 8 cut(s) 498, 557, 563, 747, 982, 1147, 1251, 1297
Alw26I GTCTC 2 cut(s) 843, 1322
AlwI GGATC 2 cut(s) 408, 1125
AlwNI CAGNNNCTG 1 cut(s) 397
Ama87I CYCGRG 1 cut(s) 677
AoxI GGCC 4 cut(s) 20, 114, 486, 1096
ApeKI GCWGC 8 cut(s) 30, 551, 554, 557, 560, 747, 752, 1368
ApoI RAATTY 6 cut(s) 181, 212, 654, 1089, 1108, 1196
Asp700I GAANNNNTTC 1 cut(s) 1278
AspLEI GCGC 4 cut(s) 74, 105, 493, 752
AspS9I GGNCC 1 cut(s) 1096
AsuC2I CCSGG 3 cut(s) 100, 485, 701
AsuHPI GGTGA 3 cut(s) 276, 367, 663
AvaI CYCGRG 1 cut(s) 677
BanI GGYRCC 1 cut(s) 71
BauI CACGAG 1 cut(s) 248
BbsI GAAGAC 3 cut(s) 273, 431, 1350
BbvI GCAGC 8 cut(s) 17, 538, 544, 566, 572, 734, 739, 1380
BccI CCATC 1 cut(s) 917
BceAI ACGGC 1 cut(s) 82
BcgI CGANNNNNNTGC 2 cut(s) 1046, 1080
BciT130I CCWGG 1 cut(s) 1413
BclI TGATCA 2 cut(s) 802, 1017
BcnI CCSGG 3 cut(s) 100, 485, 701
BcoDI GTCTC 2 cut(s) 843, 1322
BfaI CTAG 3 cut(s) 546, 1011, 1260
BfmI CTRYAG 3 cut(s) 31, 552, 558
BfoI RGCGCY 1 cut(s) 75
BglI GCCNNNNNGGC 1 cut(s) 465
Bme1390I CCNGG 4 cut(s) 100, 485, 701, 1413
BmeRI GACNNNNNGTC 1 cut(s) 856
BmeT110I CYCGRG 1 cut(s) 677
BmgBI CACGTC 1 cut(s) 1184
BmgT120I GGNCC 1 cut(s) 1096
BmiI GGNNCC 1 cut(s) 73
BmrFI CCNGG 4 cut(s) 100, 485, 701, 1413
BmsI GCATC 5 cut(s) 151, 166, 173, 272, 378
BpiI GAAGAC 3 cut(s) 273, 431, 1350
BpmI CTGGAG 1 cut(s) 867
BpuEI CTTGAG 1 cut(s) 520
BpuMI CCSGG 3 cut(s) 100, 485, 701
Bsa29I ATCGAT 1 cut(s) 1227
BsaHI GRCGYC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 1322
BsaJI CCNNGG 5 cut(s) 98, 148, 348, 483, 700
Bsc4I CCNNNNNNNGG 3 cut(s) 81, 525, 617
Bse118I RCCGGY 1 cut(s) 617
Bse1I ACTGG 3 cut(s) 402, 830, 850
Bse3DI GCAATG 2 cut(s) 323, 807
BseBI CCWGG 1 cut(s) 1413
BseCI ATCGAT 1 cut(s) 1227
BseDI CCNNGG 5 cut(s) 98, 148, 348, 483, 700
BseGI GGATG 4 cut(s) 166, 263, 370, 852
BseLI CCNNNNNNNGG 3 cut(s) 81, 525, 617
BseMI GCAATG 2 cut(s) 323, 807
BseMII CTCAG 1 cut(s) 803
BseNI ACTGG 3 cut(s) 402, 830, 850
BseRI GAGGAG 1 cut(s) 517
BseX3I CGGCCG 1 cut(s) 486
BseXI GCAGC 8 cut(s) 17, 538, 544, 566, 572, 734, 739, 1380
Bsh1236I CGCG 3 cut(s) 105, 350, 491
Bsh1285I CGRYCG 3 cut(s) 124, 192, 489
BshFI GGCC 4 cut(s) 22, 116, 488, 1098
BshNI GGYRCC 1 cut(s) 71
BshVI ATCGAT 1 cut(s) 1227
BsiEI CGRYCG 3 cut(s) 124, 192, 489
BsiHKCI CYCGRG 1 cut(s) 677
BsiSI CCGG 5 cut(s) 82, 100, 485, 618, 700
BslFI GGGAC 3 cut(s) 395, 408, 624
BslI CCNNNNNNNGG 3 cut(s) 81, 525, 617
BsmAI GTCTC 2 cut(s) 843, 1322
BsmFI GGGAC 3 cut(s) 395, 408, 624
BsmI GAATGC 3 cut(s) 328, 538, 1159
BsnI GGCC 4 cut(s) 22, 116, 488, 1098
Bso31I GGTCTC 1 cut(s) 1322
BsoBI CYCGRG 1 cut(s) 677
Bsp143I GATC 7 cut(s) 121, 189, 400, 802, 1017, 1117, 1224
BspANI GGCC 4 cut(s) 22, 116, 488, 1098
BspCNI CTCAG 1 cut(s) 802
BspDI ATCGAT 1 cut(s) 1227
BspFNI CGCG 3 cut(s) 105, 350, 491
BspHI TCATGA 1 cut(s) 226
BspLI GGNNCC 1 cut(s) 73
BspMAI CTGCAG 3 cut(s) 35, 556, 562
BspPI GGATC 2 cut(s) 408, 1125
BspT107I GGYRCC 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 1322
BsrDI GCAATG 2 cut(s) 323, 807
BsrFI RCCGGY 1 cut(s) 617
BsrI ACTGG 3 cut(s) 402, 830, 850
BssAI RCCGGY 1 cut(s) 617
BssECI CCNNGG 5 cut(s) 98, 148, 348, 483, 700
BssMI GATC 7 cut(s) 121, 189, 400, 802, 1017, 1117, 1224
BssNI GRCGYC 1 cut(s) 72
BssSI CACGAG 1 cut(s) 248
BssT1I CCWWGG 1 cut(s) 148
Bst2BI CACGAG 1 cut(s) 248
Bst2UI CCWGG 1 cut(s) 1413
Bst4CI ACNGT 4 cut(s) 221, 361, 638, 1377
Bst6I CTCTTC 1 cut(s) 644
BstACI GRCGYC 1 cut(s) 72
BstC8I GCNNGC 4 cut(s) 76, 306, 431, 600
BstDEI CTNAG 2 cut(s) 716, 789
BstDSI CCRYGG 1 cut(s) 348
BstEII GGTNACC 1 cut(s) 669
BstF5I GGATG 4 cut(s) 166, 263, 370, 852
BstFNI CGCG 3 cut(s) 105, 350, 491
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 4 cut(s) 74, 105, 493, 752
BstKTI GATC 7 cut(s) 124, 192, 403, 805, 1020, 1120, 1227
BstMAI GTCTC 2 cut(s) 843, 1322
BstMBI GATC 7 cut(s) 121, 189, 400, 802, 1017, 1117, 1224
BstMCI CGRYCG 3 cut(s) 124, 192, 489
BstMWI GCNNNNNNNGC 9 cut(s) 75, 78, 185, 325, 347, 465, 551, 557, 560
BstNI CCWGG 1 cut(s) 1413
BstPI GGTNACC 1 cut(s) 669
BstSCI CCNGG 4 cut(s) 98, 483, 699, 1411
BstSFI CTRYAG 3 cut(s) 31, 552, 558
BstUI CGCG 3 cut(s) 105, 350, 491
BstV1I GCAGC 8 cut(s) 17, 538, 544, 566, 572, 734, 739, 1380
BstV2I GAAGAC 3 cut(s) 273, 431, 1350
BstX2I RGATCY 1 cut(s) 1117
BstXI CCANNNNNNTGG 1 cut(s) 666
BstYI RGATCY 1 cut(s) 1117
BstZI CGGCCG 1 cut(s) 486
Bsu15I ATCGAT 1 cut(s) 1227
BsuRI GGCC 4 cut(s) 22, 116, 488, 1098
BsuTUI ATCGAT 1 cut(s) 1227
BtgI CCRYGG 1 cut(s) 348
BtrI CACGTC 1 cut(s) 1184
BtsCI GGATG 4 cut(s) 166, 263, 370, 852
BtsIMutI CAGTG 3 cut(s) 226, 308, 843
Cac8I GCNNGC 4 cut(s) 76, 306, 431, 600
CaiI CAGNNNCTG 1 cut(s) 397
CciI TCATGA 1 cut(s) 226
CfoI GCGC 4 cut(s) 74, 105, 493, 752
Cfr10I RCCGGY 1 cut(s) 617
Cfr13I GGNCC 1 cut(s) 1096
Cfr42I CCGCGG 1 cut(s) 351
ClaI ATCGAT 1 cut(s) 1227
Csp6I GTAC 3 cut(s) 634, 840, 949
CviAII CATG 2 cut(s) 227, 1193
CviQI GTAC 3 cut(s) 634, 840, 949
DdeI CTNAG 2 cut(s) 716, 789
DinI GGCGCC 1 cut(s) 73
DpnI GATC 7 cut(s) 123, 191, 402, 804, 1019, 1119, 1226
DpnII GATC 7 cut(s) 121, 189, 400, 802, 1017, 1117, 1224
DraI TTTAAA 1 cut(s) 523
DriI GACNNNNNGTC 1 cut(s) 856
EaeI YGGCCR 1 cut(s) 486
EagI CGGCCG 1 cut(s) 486
Eam1104I CTCTTC 1 cut(s) 644
Eam1105I GACNNNNNGTC 1 cut(s) 856
EarI CTCTTC 1 cut(s) 644
EciI GGCGGA 1 cut(s) 390
EclXI CGGCCG 1 cut(s) 486
Eco130I CCWWGG 1 cut(s) 148
Eco31I GGTCTC 1 cut(s) 1322
Eco52I CGGCCG 1 cut(s) 486
Eco57I CTGAAG 1 cut(s) 1057
Eco88I CYCGRG 1 cut(s) 677
Eco91I GGTNACC 1 cut(s) 669
EcoO65I GGTNACC 1 cut(s) 669
EcoRI GAATTC 2 cut(s) 181, 654
EcoRII CCWGG 1 cut(s) 1411
EcoT14I CCWWGG 1 cut(s) 148
EcoT22I ATGCAT 1 cut(s) 166
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 148
FaeI CATG 2 cut(s) 230, 1196
FaiI YATR 8 cut(s) 158, 228, 947, 954, 1194, 1238, 1366, 1373
FaqI GGGAC 3 cut(s) 395, 408, 624
FatI CATG 2 cut(s) 226, 1192
FauI CCCGC 1 cut(s) 83
FbaI TGATCA 2 cut(s) 802, 1017
FokI GGATG 4 cut(s) 173, 250, 377, 839
FspBI CTAG 3 cut(s) 546, 1011, 1260
GlaI GCGC 4 cut(s) 73, 104, 492, 751
GsuI CTGGAG 1 cut(s) 867
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 4 cut(s) 22, 116, 488, 1098
HapII CCGG 5 cut(s) 82, 100, 485, 618, 700
HhaI GCGC 4 cut(s) 74, 105, 493, 752
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 2 cut(s) 230, 1196
Hin6I GCGC 4 cut(s) 72, 103, 491, 750
HinP1I GCGC 4 cut(s) 72, 103, 491, 750
HincII GTYRAC 1 cut(s) 1390
HindII GTYRAC 1 cut(s) 1390
HindIII AAGCTT 1 cut(s) 496
HinfI GANTC 9 cut(s) 42, 90, 194, 251, 412, 566, 1047, 1245, 1423
HpaII CCGG 5 cut(s) 82, 100, 485, 618, 700
HphI GGTGA 3 cut(s) 276, 367, 663
Hpy166II GTNNAC 1 cut(s) 1390
Hpy188I TCNGA 9 cut(s) 41, 121, 199, 256, 744, 792, 882, 964, 998
Hpy188III TCNNGA 7 cut(s) 46, 227, 659, 677, 1222, 1260, 1283
Hpy8I GTNNAC 1 cut(s) 1390
Hpy99I CGWCG 1 cut(s) 115
HpyAV CCTTC 5 cut(s) 19, 453, 527, 923, 1081
HpyCH4III ACNGT 4 cut(s) 221, 361, 638, 1377
HpyCH4IV ACGT 1 cut(s) 1183
HpyF10VI GCNNNNNNNGC 9 cut(s) 75, 78, 185, 325, 347, 465, 551, 557, 560
HpyF3I CTNAG 2 cut(s) 716, 789
HpySE526I ACGT 1 cut(s) 1183
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 2 cut(s) 230, 1196
HspAI GCGC 4 cut(s) 72, 103, 491, 750
KasI GGCGCC 1 cut(s) 71
Ksp22I TGATCA 2 cut(s) 802, 1017
KspI CCGCGG 1 cut(s) 351
Kzo9I GATC 7 cut(s) 121, 189, 400, 802, 1017, 1117, 1224
LmnI GCTCC 2 cut(s) 199, 745
Lsp1109I GCAGC 8 cut(s) 17, 538, 544, 566, 572, 734, 739, 1380
LweI GCATC 5 cut(s) 151, 166, 173, 272, 378
MaeI CTAG 3 cut(s) 546, 1011, 1260
MaeII ACGT 1 cut(s) 1183
MaeIII GTNAC 2 cut(s) 355, 669
MalI GATC 7 cut(s) 123, 191, 402, 804, 1019, 1119, 1226
MboI GATC 7 cut(s) 121, 189, 400, 802, 1017, 1117, 1224
MfeI CAATTG 1 cut(s) 1062
MflI RGATCY 1 cut(s) 1117
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 3 cut(s) 99, 203, 260
MmeI TCCRAC 3 cut(s) 177, 234, 389
Mph1103I ATGCAT 1 cut(s) 166
MroXI GAANNNNTTC 1 cut(s) 1278
MseI TTAA 2 cut(s) 522, 1127
MslI CAYNNNNRTG 1 cut(s) 231
MspA1I CMGCKG 5 cut(s) 341, 350, 557, 563, 773
MspI CCGG 5 cut(s) 82, 100, 485, 618, 700
MspR9I CCNGG 4 cut(s) 100, 485, 701, 1413
MunI CAATTG 1 cut(s) 1062
Mva1269I GAATGC 3 cut(s) 328, 538, 1159
MvaI CCWGG 1 cut(s) 1413
MvnI CGCG 3 cut(s) 105, 350, 491
MwoI GCNNNNNNNGC 9 cut(s) 75, 78, 185, 325, 347, 465, 551, 557, 560
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 3 cut(s) 100, 485, 701
NdeII GATC 7 cut(s) 121, 189, 400, 802, 1017, 1117, 1224
NlaIII CATG 2 cut(s) 230, 1196
NlaIV GGNNCC 1 cut(s) 73
NmeAIII GCCGAG 3 cut(s) 280, 1124, 1162
NmuCI GTSAC 2 cut(s) 355, 669
NsiI ATGCAT 1 cut(s) 166
PaeR7I CTCGAG 1 cut(s) 677
PagI TCATGA 1 cut(s) 226
PctI GAATGC 3 cut(s) 328, 538, 1159
PdmI GAANNNNTTC 1 cut(s) 1278
PfeI GAWTC 6 cut(s) 42, 412, 566, 1047, 1245, 1423
Ple19I CGATCG 2 cut(s) 124, 192
PleI GAGTC 3 cut(s) 98, 202, 259
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 3 cut(s) 98, 202, 259
Psp6I CCWGG 1 cut(s) 1411
PspEI GGTNACC 1 cut(s) 669
PspGI CCWGG 1 cut(s) 1411
PspN4I GGNNCC 1 cut(s) 73
PspPI GGNCC 1 cut(s) 1096
PstI CTGCAG 3 cut(s) 35, 556, 562
PstNI CAGNNNCTG 1 cut(s) 397
PsuI RGATCY 1 cut(s) 1117
PvuI CGATCG 2 cut(s) 124, 192
PvuII CAGCTG 2 cut(s) 557, 563
RsaI GTAC 3 cut(s) 635, 841, 950
RsaNI GTAC 3 cut(s) 634, 840, 949
RseI CAYNNNNRTG 1 cut(s) 231
SacII CCGCGG 1 cut(s) 351
SaqAI TTAA 2 cut(s) 522, 1127
Sau3AI GATC 7 cut(s) 121, 189, 400, 802, 1017, 1117, 1224
Sau96I GGNCC 1 cut(s) 1096
SchI GAGTC 3 cut(s) 99, 203, 260
ScrFI CCNGG 4 cut(s) 100, 485, 701, 1413
SfaNI GCATC 5 cut(s) 151, 166, 173, 272, 378
SfcI CTRYAG 3 cut(s) 31, 552, 558
SfoI GGCGCC 1 cut(s) 73
Sfr274I CTCGAG 1 cut(s) 677
Sfr303I CCGCGG 1 cut(s) 351
SgrBI CCGCGG 1 cut(s) 351
SlaI CTCGAG 1 cut(s) 677
SmiMI CAYNNNNRTG 1 cut(s) 231
SmlI CTYRAG 2 cut(s) 499, 677
SmoI CTYRAG 2 cut(s) 499, 677
SspDI GGCGCC 1 cut(s) 71
SspMI CTAG 3 cut(s) 546, 1011, 1260
StyD4I CCNGG 4 cut(s) 98, 483, 699, 1411
StyI CCWWGG 1 cut(s) 148
TaaI ACNGT 4 cut(s) 221, 361, 638, 1377
TaiI ACGT 1 cut(s) 1186
TaqI TCGA 6 cut(s) 17, 192, 678, 1056, 1221, 1227
TatI WGTACW 1 cut(s) 948
TauI GCSGC 5 cut(s) 72, 81, 322, 491, 773
TfiI GAWTC 6 cut(s) 42, 412, 566, 1047, 1245, 1423
Tru1I TTAA 2 cut(s) 522, 1127
Tru9I TTAA 2 cut(s) 522, 1127
TscAI CASTG 3 cut(s) 226, 315, 850
TseFI GTSAC 2 cut(s) 355, 669
TseI GCWGC 8 cut(s) 30, 551, 554, 557, 560, 747, 752, 1368
Tsp45I GTSAC 2 cut(s) 355, 669
TspDTI ATGAA 8 cut(s) 215, 381, 950, 1112, 1166, 1179, 1209, 1307
TspGWI ACGGA 2 cut(s) 124, 621
TspRI CASTG 3 cut(s) 226, 315, 850
XapI RAATTY 6 cut(s) 181, 212, 654, 1089, 1108, 1196
XbaI TCTAGA 1 cut(s) 1259
XcmI CCANNNNNNNNNTGG 1 cut(s) 460
XhoI CTCGAG 1 cut(s) 677
XmnI GAANNNNTTC 1 cut(s) 1278
XspI CTAG 3 cut(s) 546, 1011, 1260
Zsp2I ATGCAT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.