Rh2AG614400

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0011589
Physical Location & Seq
Reverse (-)
83982997 .. 83987232
4236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG614400.1

Sequence Viewer

Length: 942 bp
ATGGGAGCTTGCGCTTCCCGGCCCAAGGGCTGGGCCTTGAGATTGTCAAAGAAGAAGAAGAATGGCCGCCAAAGACGAATCATTCGGAGGCGTGTTTCCTCCTCCAACCGATCTAACTTCAACCCACAACTCCAAGGAAGCATGGACGGGGCTTGGTTTGATTGTACTTCAGTGATGGATTCGGAGCGAGGAGACGACGAGTTCTACAGTGTCTATGATGATGTGGTGTCTTTGAATGGGTCGGAGAGTGCGATACAGAGAACGAGGGAAGGCGGTGCGGATGAGAGTGATAGAATGCAAGGGTTGGTGGTTGTTCAGAATGCTTGCCTGCCTTGTCTACCTTCAACTAGCCCCTTGTCAGTTGATAACAAACTGAGTTTTAGGAAGAGATTGGGGCCTTCTAGGCTCTCGTTTAAGTGGAGGGAAGGGCATGCTGCTCCGGCTGATCCCACATTAGTTTCGCCCAGGGCGGTTGTTAAAAGACCAATAGCAGGTTCGACAATTCCACACTGCCCGATGGGCAAGAGAATGCCAGATTGTTGGTCACCTCTTGAGCCAAACACTTTCAAAGTCCGGGGGAAGAATTATCTTAGGGACAAAAAGAAAGAGTTAGCTCCAGATTGCGCCGCGTATTATCCTTTTGGGGTTGATATTTTTTTATCTCAGAGGAAGATTGATCATATTGCTCGCTTTGTGGAACTTCCTGTTGTGAATGTGGATGGAGATGTCCCTTCTATTCTTGTTGTAAATGTTCAGATACCTTTGTACCCTATCTCTATGTTTCAGAGCGAACATGATGGAGAAGGAATGAATTTGGTTATGTATTTTAAGCTTTCTGAAAGTTACTCAAAAGACCTTCCAGCTCATTTCCGCGAAAGTTTAACTGTAAGATCCGTGAATTATAAAAATATATGGTCTCTCATGTCACACTGGATCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

313

Amino Acids

35.33

Weight (kDa)

9.42

Isoelectric Point (pI)

56.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 181 - 285 6.7e-22 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 903
Acc36I ACCTGC 1 cut(s) 482
AccB7I CCANNNNNTGG 1 cut(s) 30
AccI GTMKAC 1 cut(s) 337
AccII CGCG 2 cut(s) 629, 873
AciI CCGC 6 cut(s) 67, 273, 278, 470, 627, 871
AclWI GGATC 3 cut(s) 440, 885, 941
AcoI YGGCCR 1 cut(s) 64
AcsI RAATTY 1 cut(s) 811
AcuI CTGAAG 1 cut(s) 153
AfaI GTAC 2 cut(s) 166, 767
AfiI CCNNNNNNNGG 3 cut(s) 25, 30, 491
AgsI TTSAA 4 cut(s) 121, 235, 345, 568
AjnI CCWGG 1 cut(s) 464
AjuI GAANNNNNNNTTGG 2 cut(s) 478, 510
AluBI AGCT 4 cut(s) 8, 614, 832, 863
AluI AGCT 4 cut(s) 8, 614, 832, 863
Alw26I GTCTC 2 cut(s) 186, 921
AlwI GGATC 3 cut(s) 440, 885, 941
AoxI GGCC 4 cut(s) 20, 33, 64, 395
ApeKI GCWGC 1 cut(s) 434
ApoI RAATTY 1 cut(s) 811
ArsI GACNNNNNNTTYG 2 cut(s) 66, 98
AspLEI GCGC 2 cut(s) 14, 626
AspS9I GGNCC 3 cut(s) 21, 33, 395
AsuC2I CCSGG 2 cut(s) 19, 575
AsuHPI GGTGA 1 cut(s) 537
BaeI ACNNNNGTAYC 4 cut(s) 749, 749, 782, 782
BbvI GCAGC 1 cut(s) 421
BccI CCATC 4 cut(s) 169, 511, 713, 791
BciT130I CCWGG 1 cut(s) 466
BclI TGATCA 1 cut(s) 676
BcnI CCSGG 2 cut(s) 19, 575
BcoDI GTCTC 2 cut(s) 186, 921
BfaI CTAG 2 cut(s) 348, 402
BfmI CTRYAG 1 cut(s) 205
BfuAI ACCTGC 1 cut(s) 482
BglI GCCNNNNNGGC 2 cut(s) 403, 519
BisI GCNGC 3 cut(s) 67, 435, 627
BlsI GCNGC 3 cut(s) 68, 436, 628
Bme1390I CCNGG 3 cut(s) 19, 466, 575
BmgT120I GGNCC 3 cut(s) 21, 33, 395
BmiI GGNNCC 1 cut(s) 396
BmrFI CCNGG 3 cut(s) 19, 466, 575
BpmI CTGGAG 1 cut(s) 600
BpuEI CTTGAG 2 cut(s) 58, 572
BpuMI CCSGG 2 cut(s) 19, 575
BsaI GGTCTC 1 cut(s) 921
BsaJI CCNNGG 5 cut(s) 24, 133, 464, 465, 574
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 30, 491
Bse1I ACTGG 1 cut(s) 935
BseBI CCWGG 1 cut(s) 466
BseDI CCNNGG 5 cut(s) 24, 133, 464, 465, 574
BseGI GGATG 2 cut(s) 286, 724
BseLI CCNNNNNNNGG 3 cut(s) 25, 30, 491
BseMII CTCAG 2 cut(s) 365, 677
BseNI ACTGG 1 cut(s) 935
BseRI GAGGAG 2 cut(s) 91, 204
BseXI GCAGC 1 cut(s) 421
BseYI CCCAGC 1 cut(s) 30
Bsh1236I CGCG 2 cut(s) 629, 873
BshFI GGCC 4 cut(s) 22, 35, 66, 397
BsiSI CCGG 3 cut(s) 19, 440, 574
BslFI GGGAC 2 cut(s) 608, 713
BslI CCNNNNNNNGG 3 cut(s) 25, 30, 491
BsmAI GTCTC 2 cut(s) 186, 921
BsmBI CGTCTC 1 cut(s) 186
BsmFI GGGAC 2 cut(s) 608, 713
BsmI GAATGC 3 cut(s) 300, 325, 534
BsnI GGCC 4 cut(s) 22, 35, 66, 397
Bso31I GGTCTC 1 cut(s) 921
Bsp143I GATC 5 cut(s) 110, 445, 676, 890, 933
BspACI CCGC 6 cut(s) 67, 273, 278, 470, 627, 871
BspANI GGCC 4 cut(s) 22, 35, 66, 397
BspCNI CTCAG 2 cut(s) 366, 676
BspFNI CGCG 2 cut(s) 629, 873
BspLI GGNNCC 1 cut(s) 396
BspMI ACCTGC 1 cut(s) 482
BspPI GGATC 3 cut(s) 440, 885, 941
BspTNI GGTCTC 1 cut(s) 921
BsrI ACTGG 1 cut(s) 935
BssECI CCNNGG 5 cut(s) 24, 133, 464, 465, 574
BssMI GATC 5 cut(s) 110, 445, 676, 890, 933
BssT1I CCWWGG 2 cut(s) 24, 133
Bst2UI CCWGG 1 cut(s) 466
Bst4CI ACNGT 2 cut(s) 209, 886
Bst6I CTCTTC 1 cut(s) 380
BstC8I GCNNGC 5 cut(s) 10, 325, 329, 432, 688
BstDEI CTNAG 3 cut(s) 374, 590, 663
BstEII GGTNACC 1 cut(s) 543
BstF5I GGATG 2 cut(s) 286, 724
BstFNI CGCG 2 cut(s) 629, 873
BstHHI GCGC 2 cut(s) 14, 626
BstKTI GATC 5 cut(s) 113, 448, 679, 893, 936
BstMAI GTCTC 2 cut(s) 186, 921
BstMBI GATC 5 cut(s) 110, 445, 676, 890, 933
BstMWI GCNNNNNNNGC 3 cut(s) 403, 440, 519
BstNI CCWGG 1 cut(s) 466
BstNSI RCATGY 1 cut(s) 434
BstPI GGTNACC 1 cut(s) 543
BstSCI CCNGG 3 cut(s) 17, 464, 573
BstSFI CTRYAG 1 cut(s) 205
BstUI CGCG 2 cut(s) 629, 873
BstV1I GCAGC 1 cut(s) 421
BstX2I RGATCY 2 cut(s) 890, 933
BstXI CCANNNNNNTGG 1 cut(s) 540
BstYI RGATCY 2 cut(s) 890, 933
BsuRI GGCC 4 cut(s) 22, 35, 66, 397
BtsCI GGATG 2 cut(s) 286, 724
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 4 cut(s) 177, 214, 508, 928
BveI ACCTGC 1 cut(s) 482
Cac8I GCNNGC 5 cut(s) 10, 325, 329, 432, 688
CfoI GCGC 2 cut(s) 14, 626
Cfr13I GGNCC 3 cut(s) 21, 33, 395
Csp6I GTAC 2 cut(s) 165, 766
CviAII CATG 4 cut(s) 142, 431, 794, 922
CviQI GTAC 2 cut(s) 165, 766
DdeI CTNAG 3 cut(s) 374, 590, 663
DpnI GATC 5 cut(s) 112, 447, 678, 892, 935
DpnII GATC 5 cut(s) 110, 445, 676, 890, 933
EaeI YGGCCR 1 cut(s) 64
Eam1104I CTCTTC 1 cut(s) 380
EarI CTCTTC 1 cut(s) 380
Eco130I CCWWGG 2 cut(s) 24, 133
Eco31I GGTCTC 1 cut(s) 921
Eco57I CTGAAG 1 cut(s) 153
Eco91I GGTNACC 1 cut(s) 543
EcoO109I RGGNCCY 1 cut(s) 395
EcoO65I GGTNACC 1 cut(s) 543
EcoRII CCWGG 1 cut(s) 464
EcoT14I CCWWGG 2 cut(s) 24, 133
ErhI CCWWGG 2 cut(s) 24, 133
Esp3I CGTCTC 1 cut(s) 186
FaeI CATG 4 cut(s) 145, 434, 797, 925
FaqI GGGAC 2 cut(s) 608, 713
FatI CATG 4 cut(s) 141, 430, 793, 921
FbaI TGATCA 1 cut(s) 676
FblI GTMKAC 1 cut(s) 337
Fnu4HI GCNGC 3 cut(s) 67, 435, 627
FokI GGATG 2 cut(s) 293, 731
Fsp4HI GCNGC 3 cut(s) 67, 435, 627
FspBI CTAG 2 cut(s) 348, 402
GlaI GCGC 2 cut(s) 13, 625
GluI GCNGC 3 cut(s) 67, 435, 627
GsaI CCCAGC 1 cut(s) 34
GsuI CTGGAG 1 cut(s) 600
HaeIII GGCC 4 cut(s) 22, 35, 66, 397
HapII CCGG 3 cut(s) 19, 440, 574
HhaI GCGC 2 cut(s) 14, 626
Hin1II CATG 4 cut(s) 145, 434, 797, 925
Hin6I GCGC 2 cut(s) 12, 624
HinP1I GCGC 2 cut(s) 12, 624
HindIII AAGCTT 1 cut(s) 830
HinfI GANTC 2 cut(s) 78, 179
HpaII CCGG 3 cut(s) 19, 440, 574
HphI GGTGA 1 cut(s) 537
Hpy166II GTNNAC 1 cut(s) 338
Hpy188I TCNGA 8 cut(s) 87, 184, 244, 318, 666, 756, 786, 838
Hpy188III TCNNGA 2 cut(s) 551, 617
Hpy8I GTNNAC 1 cut(s) 338
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 7 cut(s) 263, 351, 408, 419, 741, 797, 866
HpyCH4III ACNGT 2 cut(s) 209, 886
HpyCH4V TGCA 1 cut(s) 298
HpyF10VI GCNNNNNNNGC 3 cut(s) 403, 440, 519
HpyF3I CTNAG 3 cut(s) 374, 590, 663
Hsp92II CATG 4 cut(s) 145, 434, 797, 925
HspAI GCGC 2 cut(s) 12, 624
Ksp22I TGATCA 1 cut(s) 676
Kzo9I GATC 5 cut(s) 110, 445, 676, 890, 933
LmnI GCTCC 4 cut(s) 5, 184, 442, 619
Lsp1109I GCAGC 1 cut(s) 421
MaeI CTAG 2 cut(s) 348, 402
MaeIII GTNAC 3 cut(s) 543, 842, 924
MalI GATC 5 cut(s) 112, 447, 678, 892, 935
MboI GATC 5 cut(s) 110, 445, 676, 890, 933
MboII GAAGA 6 cut(s) 64, 67, 70, 397, 592, 682
MflI RGATCY 2 cut(s) 890, 933
MluCI AATT 4 cut(s) 501, 583, 811, 898
MmeI TCCRAC 2 cut(s) 129, 222
MnlI CCTC 8 cut(s) 81, 109, 112, 182, 258, 414, 558, 660
MseI TTAA 4 cut(s) 414, 477, 828, 881
MspI CCGG 3 cut(s) 19, 440, 574
MspR9I CCNGG 3 cut(s) 19, 466, 575
Mva1269I GAATGC 3 cut(s) 300, 325, 534
MvaI CCWGG 1 cut(s) 466
MvnI CGCG 2 cut(s) 629, 873
MwoI GCNNNNNNNGC 3 cut(s) 403, 440, 519
NciI CCSGG 2 cut(s) 19, 575
NdeII GATC 5 cut(s) 110, 445, 676, 890, 933
NlaIII CATG 4 cut(s) 145, 434, 797, 925
NlaIV GGNNCC 1 cut(s) 396
NmuCI GTSAC 2 cut(s) 543, 924
NspI RCATGY 1 cut(s) 434
PaeI GCATGC 1 cut(s) 434
PasI CCCWGGG 1 cut(s) 465
PcsI WCGNNNNNNNCGW 1 cut(s) 248
PctI GAATGC 3 cut(s) 300, 325, 534
PfeI GAWTC 2 cut(s) 78, 179
PflMI CCANNNNNTGG 1 cut(s) 30
PkrI GCNGC 3 cut(s) 68, 436, 628
PsiI TTATAA 1 cut(s) 903
Psp6I CCWGG 1 cut(s) 464
PspEI GGTNACC 1 cut(s) 543
PspFI CCCAGC 1 cut(s) 30
PspGI CCWGG 1 cut(s) 464
PspN4I GGNNCC 1 cut(s) 396
PspPI GGNCC 3 cut(s) 21, 33, 395
PsuI RGATCY 2 cut(s) 890, 933
RsaI GTAC 2 cut(s) 166, 767
RsaNI GTAC 2 cut(s) 165, 766
SaqAI TTAA 4 cut(s) 414, 477, 828, 881
SatI GCNGC 3 cut(s) 67, 435, 627
Sau3AI GATC 5 cut(s) 110, 445, 676, 890, 933
Sau96I GGNCC 3 cut(s) 21, 33, 395
ScrFI CCNGG 3 cut(s) 19, 466, 575
SetI ASST 9 cut(s) 10, 343, 496, 550, 616, 763, 834, 858, 865
SfcI CTRYAG 1 cut(s) 205
SmlI CTYRAG 2 cut(s) 37, 551
SmoI CTYRAG 2 cut(s) 37, 551
SphI GCATGC 1 cut(s) 434
Sse9I AATT 4 cut(s) 501, 583, 811, 898
SsiI CCGC 6 cut(s) 67, 273, 278, 470, 627, 871
SspMI CTAG 2 cut(s) 348, 402
StyD4I CCNGG 3 cut(s) 17, 464, 573
StyI CCWWGG 2 cut(s) 24, 133
TaaI ACNGT 2 cut(s) 209, 886
TaqI TCGA 1 cut(s) 497
TasI AATT 4 cut(s) 501, 583, 811, 898
TatI WGTACW 1 cut(s) 164
TauI GCSGC 2 cut(s) 69, 629
TfiI GAWTC 2 cut(s) 78, 179
Tru1I TTAA 4 cut(s) 414, 477, 828, 881
Tru9I TTAA 4 cut(s) 414, 477, 828, 881
TscAI CASTG 4 cut(s) 177, 214, 515, 935
TseFI GTSAC 2 cut(s) 543, 924
TseI GCWGC 1 cut(s) 434
Tsp45I GTSAC 2 cut(s) 543, 924
TspDTI ATGAA 1 cut(s) 824
TspGWI ACGGA 1 cut(s) 883
TspRI CASTG 4 cut(s) 177, 214, 515, 935
Van91I CCANNNNNTGG 1 cut(s) 30
XapI RAATTY 1 cut(s) 811
XceI RCATGY 1 cut(s) 434
XmiI GTMKAC 1 cut(s) 337
XspI CTAG 2 cut(s) 348, 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.