FvH4_6g49960

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012381
Physical Location & Seq
Forward (+)
37297080 .. 37300107
3028 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g49960.t1

Sequence Viewer

Length: 1638 bp
ATGGCTGGCACAGCAAGAAAGAGACTCGGCAACTCATGCATTCTTCTCATAATGATTTCTGGTATGGAAAGATTTGCATACAAAGGAGTGGCATCCAATCTGATAACTTATCTAACGGATGTAGTAAAGATGAGCAACTCTTCTGCGGCGAAGACGGTGAACAGTTGGTGCGGATTCACATCCATGGTTCCACTCTTGATTGCACCCTTTGCTGATTCTTACTGGGATAGATATTACACCATTGTAGCTTCTTCTCTTGTCTATGTTGCCGGGCTTGTGGCATTGACATCAACAGCAATAATGTCCCGGGCATCCACGGATCAAGCAAACAAAACAAGTTCATCTTCATTTCTGTTTTGGTCATTGTATTTGATTTCTCTTGGTCAAGGTGGGTATAATCCATCTTTACAAGCTTTCGGAGCAGATCAACTTGACAGTGAAGAGGAAGAGTTGCCTACTAGCGAAGATGACAAAAAACCCAACAAAAAGAGTTTGTTCTTTCAATGGTGGTACTTTGGTGTTTGTAGTGGCACCGTCTTGGGTATCAGTGTTATGTCCTACATTCAAGATAATTTTGGTTGGATACTAGGTTTTGCTATTCCCATGATTTCTATGGTCACATCAGTCGTAGTTTTCTCGTGTGGAAGCCGAATTTATACATATAAACAGAGGAATGATGATTACAAGCCTGTTGTGAGCATCCTTCAAGTTATTAAGGCAGCTGCATGGAAATTACTGAATTGCAAAGTCAAGTTGCCCAATGAAAATAAAGTTACTGAGCTAGAGCTTCAAGGGAAACCGCTCTGTCTTCGGAACTTTGGCAGCAATGAAGGCTTGAACAATGAACATCCCAAAAGCAGCGTCTACAAGTTCGAAAACGCAAAAGTTATTCTTAGGCTTTTGCCCATTTGGCTAATGCTGTTAATGTTTGCAGTGATTTTCCAGCAACCTTCGACATTCTTCACCAAACAAGGCATGACAATGAAGAGGAACATTGGAAGCAATTTTCTGATTCCACCGGCAACACTACAAAGTGCTATTGCACTTTCTATAGTCCTATTGATGCCTCTTTATGACAAAGTTTTGATCCCAATTACTAAGGTCCTGACTTGTAACGAAAAGGGTATCACCGTAATGCAAAGGATGGGAATCGGGATATTCACTTCGGTCATAGCACTGATCATTGCAGCAATTGTGGAGACAAGAAGGCTCCATATCAGCAGAAAGATGGAAATGCTAGGATCTCAATCTGAGACAGTTCCACTTAGCATATTCTGGTTGCTGCCACAATACATTCTTCTAGGAATTTCAGACATTTTCACTGTGGTTGGGATGCAAGAGTTCTTCTACTCTGAATTTCCCATTAAAATGAGAACAATGGCCTTTGCACTATACACCAGTGTTTTTGGGGTTGGGAGTTACTTGAGTGCCTTTTTGATATCCACAGTTGAGACCATCACAAGTTCAAATGGAAATAAGAGCTGGTTCTGTGATGATATGAGTGAAGCTAGGCTAGACAAGTACTACTGGTTTTTAGCAGCATCAAGTGCACTGAGCTTTCTGTTTTTTGTGATTATTTCATCATGTTACACAAGTAAAAGGGATTTGGACTTGGACAGTAATGAAAGTTGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0043207 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

546

Amino Acids

60.92

Weight (kDa)

8.8

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 27 - 475 1.1e-08 Major Facilitator Superfamily
PTR2 PF00854 79 - 495 1.3e-76 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012381)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01350 AT5G14940
fragaria_vesca FvH4_6g49960
malus_domestica MD09G1040700.v1.1 MD17G1041000.v1.1
prunus_persica Prupe.3G277200_v2.0.a1 Prupe.3G277200_v2.0.a1
pyrus_communis pycom111g03160 pycom17g03740
rosa_chinensis RchiOBHm_Chr2g0170141
rosa_laevigata RLG00000021928
rosa_multiflora Rmu_sc0001605.1_g000022
rosa_roxburghii Rroxscaffold_2G00081560
rosa_rugosa Rorug02G0546100.1
rosa_samantha Rh2BG629700 Rh2CG599200 Rh2DG641600
rosa_wichuraiana Rw0G018340 Rw2G051190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 530
AccBSI CCGCTC 1 cut(s) 802
AccI GTMKAC 1 cut(s) 864
AciI CCGC 3 cut(s) 146, 171, 800
AclWI GGATC 3 cut(s) 327, 1081, 1249
AcsI RAATTY 3 cut(s) 651, 1305, 1355
AfaI GTAC 2 cut(s) 512, 1523
AgsI TTSAA 6 cut(s) 503, 566, 707, 791, 838, 1467
AluBI AGCT 8 cut(s) 248, 413, 722, 781, 787, 1482, 1508, 1557
AluI AGCT 8 cut(s) 248, 413, 722, 781, 787, 1482, 1508, 1557
Alw21I GWGCWC 1 cut(s) 1552
Alw26I GTCTC 4 cut(s) 16, 1193, 1247, 1445
Alw44I GTGCAC 1 cut(s) 1548
AlwI GGATC 3 cut(s) 327, 1081, 1249
Ama87I CYCGRG 1 cut(s) 306
AoxI GGCC 1 cut(s) 1380
ApaLI GTGCAC 1 cut(s) 1548
ApeKI GCWGC 7 cut(s) 719, 722, 822, 858, 1187, 1282, 1538
ApoI RAATTY 3 cut(s) 651, 1305, 1355
ArsI GACNNNNNNTTYG 2 cut(s) 846, 878
AspS9I GGNCC 1 cut(s) 1102
AsuC2I CCSGG 3 cut(s) 271, 307, 308
AsuHPI GGTGA 3 cut(s) 169, 955, 1120
AsuII TTCGAA 1 cut(s) 873
AvaI CYCGRG 1 cut(s) 306
AvaII GGWCC 1 cut(s) 1102
BaeGI GKGCMC 1 cut(s) 1552
BaeI ACNNNNGTAYC 2 cut(s) 502, 535
BanI GGYRCC 1 cut(s) 530
BauI CACGAG 1 cut(s) 637
BbsI GAAGAC 2 cut(s) 158, 800
Bbv12I GWGCWC 1 cut(s) 1552
BbvI GCAGC 7 cut(s) 709, 731, 834, 870, 1199, 1269, 1550
BccI CCATC 4 cut(s) 409, 1138, 1222, 1463
BciVI GTATCC 1 cut(s) 576
BclI TGATCA 1 cut(s) 1179
BcnI CCSGG 3 cut(s) 271, 307, 308
BcoDI GTCTC 4 cut(s) 16, 1193, 1247, 1445
BfaI CTAG 7 cut(s) 459, 587, 782, 1238, 1301, 1509, 1514
BfmI CTRYAG 1 cut(s) 1050
BfuI GTATCC 1 cut(s) 576
BglI GCCNNNNNGGC 1 cut(s) 910
BisI GCNGC 8 cut(s) 147, 720, 723, 823, 859, 1188, 1283, 1539
BlsI GCNGC 8 cut(s) 148, 721, 724, 824, 860, 1189, 1284, 1540
BmcAI AGTACT 1 cut(s) 1523
Bme1390I CCNGG 3 cut(s) 271, 307, 308
Bme18I GGWCC 1 cut(s) 1102
BmeT110I CYCGRG 1 cut(s) 306
BmgT120I GGNCC 1 cut(s) 1102
BmiI GGNNCC 3 cut(s) 189, 532, 1211
BmrFI CCNGG 3 cut(s) 271, 307, 308
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 6 cut(s) 101, 320, 708, 1053, 1323, 1550
BmuI ACTGGG 1 cut(s) 232
BpiI GAAGAC 2 cut(s) 158, 800
Bpu14I TTCGAA 1 cut(s) 873
BpuEI CTTGAG 1 cut(s) 1444
BpuMI CCSGG 3 cut(s) 271, 307, 308
BsaBI GATNNNNATC 3 cut(s) 178, 1148, 1246
BsaI GGTCTC 1 cut(s) 1445
BsaJI CCNNGG 3 cut(s) 183, 306, 315
Bse118I RCCGGY 1 cut(s) 1018
Bse1I ACTGG 3 cut(s) 227, 1398, 1532
Bse3DI GCAATG 2 cut(s) 832, 1182
Bse8I GATNNNNATC 3 cut(s) 178, 1148, 1246
BseDI CCNNGG 3 cut(s) 183, 306, 315
BseGI GGATG 8 cut(s) 92, 124, 179, 311, 699, 847, 1149, 1338
BseJI GATNNNNATC 3 cut(s) 178, 1148, 1246
BseMI GCAATG 2 cut(s) 832, 1182
BseMII CTCAG 3 cut(s) 768, 1242, 1544
BseNI ACTGG 3 cut(s) 227, 1398, 1532
BseSI GKGCMC 1 cut(s) 1552
BseXI GCAGC 7 cut(s) 709, 731, 834, 870, 1199, 1269, 1550
BshFI GGCC 1 cut(s) 1382
BshNI GGYRCC 1 cut(s) 530
BsiHKAI GWGCWC 1 cut(s) 1552
BsiHKCI CYCGRG 1 cut(s) 306
BsiSI CCGG 3 cut(s) 270, 307, 1019
BslFI GGGAC 1 cut(s) 289
BsmAI GTCTC 4 cut(s) 16, 1193, 1247, 1445
BsmFI GGGAC 1 cut(s) 289
BsmI GAATGC 1 cut(s) 39
BsnI GGCC 1 cut(s) 1382
Bso31I GGTCTC 1 cut(s) 1445
BsoBI CYCGRG 1 cut(s) 306
Bsp119I TTCGAA 1 cut(s) 873
Bsp1286I GDGCHC 1 cut(s) 1552
Bsp143I GATC 5 cut(s) 319, 424, 1086, 1179, 1241
Bsp19I CCATGG 1 cut(s) 183
BspACI CCGC 3 cut(s) 146, 171, 800
BspANI GGCC 1 cut(s) 1382
BspCNI CTCAG 3 cut(s) 769, 1243, 1545
BspLI GGNNCC 3 cut(s) 189, 532, 1211
BspPI GGATC 3 cut(s) 327, 1081, 1249
BspT104I TTCGAA 1 cut(s) 873
BspT107I GGYRCC 1 cut(s) 530
BspTNI GGTCTC 1 cut(s) 1445
BsrBI CCGCTC 1 cut(s) 802
BsrDI GCAATG 2 cut(s) 832, 1182
BsrFI RCCGGY 1 cut(s) 1018
BsrI ACTGG 3 cut(s) 227, 1398, 1532
BssAI RCCGGY 1 cut(s) 1018
BssECI CCNNGG 3 cut(s) 183, 306, 315
BssMI GATC 5 cut(s) 319, 424, 1086, 1179, 1241
BssSI CACGAG 1 cut(s) 637
BssT1I CCWWGG 1 cut(s) 183
Bst2BI CACGAG 1 cut(s) 637
Bst4CI ACNGT 9 cut(s) 157, 164, 437, 535, 1132, 1258, 1324, 1447, 1619
Bst6I CTCTTC 4 cut(s) 145, 435, 441, 980
BstAPI GCANNNNNTGC 3 cut(s) 36, 209, 1547
BstBI TTCGAA 1 cut(s) 873
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 6 cut(s) 777, 893, 1098, 1251, 1265, 1553
BstDSI CCRYGG 2 cut(s) 183, 315
BstF5I GGATG 8 cut(s) 92, 124, 179, 311, 699, 847, 1149, 1338
BstKTI GATC 5 cut(s) 322, 427, 1089, 1182, 1244
BstMAI GTCTC 4 cut(s) 16, 1193, 1247, 1445
BstMBI GATC 5 cut(s) 319, 424, 1086, 1179, 1241
BstMWI GCNNNNNNNGC 7 cut(s) 11, 36, 209, 419, 831, 910, 1547
BstSCI CCNGG 3 cut(s) 269, 305, 306
BstSFI CTRYAG 1 cut(s) 1050
BstSLI GKGCMC 1 cut(s) 1552
BstV1I GCAGC 7 cut(s) 709, 731, 834, 870, 1199, 1269, 1550
BstV2I GAAGAC 2 cut(s) 158, 800
BstX2I RGATCY 1 cut(s) 1241
BstYI RGATCY 1 cut(s) 1241
BsuI GTATCC 1 cut(s) 576
BsuRI GGCC 1 cut(s) 1382
BtgI CCRYGG 2 cut(s) 183, 315
BtsCI GGATG 8 cut(s) 92, 124, 179, 311, 699, 847, 1149, 1338
BtsI GCAGTG 1 cut(s) 939
BtsIMutI CAGTG 7 cut(s) 442, 553, 939, 1175, 1320, 1405, 1550
Cac8I GCNNGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 1018
Cfr13I GGNCC 1 cut(s) 1102
Cfr9I CCCGGG 1 cut(s) 306
CseI GACGC 1 cut(s) 850
Csp6I GTAC 2 cut(s) 511, 1522
CviAII CATG 6 cut(s) 36, 184, 604, 726, 976, 1584
CviQI GTAC 2 cut(s) 511, 1522
DdeI CTNAG 6 cut(s) 777, 893, 1098, 1251, 1265, 1553
DpnI GATC 5 cut(s) 321, 426, 1088, 1181, 1243
DpnII GATC 5 cut(s) 319, 424, 1086, 1179, 1241
Eam1104I CTCTTC 4 cut(s) 145, 435, 441, 980
EarI CTCTTC 4 cut(s) 145, 435, 441, 980
Eco130I CCWWGG 1 cut(s) 183
Eco31I GGTCTC 1 cut(s) 1445
Eco32I GATATC 1 cut(s) 1440
Eco47I GGWCC 1 cut(s) 1102
Eco88I CYCGRG 1 cut(s) 306
EcoO109I RGGNCCY 1 cut(s) 1102
EcoRV GATATC 1 cut(s) 1440
EcoT14I CCWWGG 1 cut(s) 183
EcoT22I ATGCAT 1 cut(s) 41
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 6 cut(s) 39, 187, 607, 729, 979, 1587
FalI AAGNNNNNCTT 4 cut(s) 328, 360, 876, 908
FaqI GGGAC 1 cut(s) 289
FatI CATG 6 cut(s) 35, 183, 603, 725, 975, 1583
FbaI TGATCA 1 cut(s) 1179
FblI GTMKAC 1 cut(s) 864
Fnu4HI GCNGC 8 cut(s) 147, 720, 723, 823, 859, 1188, 1283, 1539
FokI GGATG 8 cut(s) 79, 131, 166, 298, 686, 834, 1156, 1345
Fsp4HI GCNGC 8 cut(s) 147, 720, 723, 823, 859, 1188, 1283, 1539
FspBI CTAG 7 cut(s) 459, 587, 782, 1238, 1301, 1509, 1514
GluI GCNGC 8 cut(s) 147, 720, 723, 823, 859, 1188, 1283, 1539
HaeIII GGCC 1 cut(s) 1382
HapII CCGG 3 cut(s) 270, 307, 1019
HgaI GACGC 1 cut(s) 850
Hin1II CATG 6 cut(s) 39, 187, 607, 729, 979, 1587
HindIII AAGCTT 1 cut(s) 411
HinfI GANTC 5 cut(s) 24, 174, 215, 1012, 1149
HpaII CCGG 3 cut(s) 270, 307, 1019
HphI GGTGA 3 cut(s) 169, 955, 1120
Hpy166II GTNNAC 3 cut(s) 160, 865, 1550
Hpy188I TCNGA 7 cut(s) 102, 419, 813, 1011, 1252, 1312, 1354
Hpy188III TCNNGA 4 cut(s) 196, 566, 1105, 1153
Hpy8I GTNNAC 3 cut(s) 160, 865, 1550
HpyAV CCTTC 4 cut(s) 713, 824, 960, 1200
HpyCH4III ACNGT 9 cut(s) 157, 164, 437, 535, 1132, 1258, 1324, 1447, 1619
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 36, 209, 419, 831, 910, 1547
HpyF3I CTNAG 6 cut(s) 777, 893, 1098, 1251, 1265, 1553
Hsp92II CATG 6 cut(s) 39, 187, 607, 729, 979, 1587
Ksp22I TGATCA 1 cut(s) 1179
Kzo9I GATC 5 cut(s) 319, 424, 1086, 1179, 1241
LmnI GCTCC 2 cut(s) 419, 1215
Lsp1109I GCAGC 7 cut(s) 709, 731, 834, 870, 1199, 1269, 1550
LweI GCATC 6 cut(s) 101, 320, 708, 1053, 1323, 1550
MaeI CTAG 7 cut(s) 459, 587, 782, 1238, 1301, 1509, 1514
MaeIII GTNAC 5 cut(s) 616, 772, 1112, 1418, 1586
MalI GATC 5 cut(s) 321, 426, 1088, 1181, 1243
MbiI CCGCTC 1 cut(s) 802
MboI GATC 5 cut(s) 319, 424, 1086, 1179, 1241
MfeI CAATTG 1 cut(s) 1191
MflI RGATCY 1 cut(s) 1241
MhlI GDGCHC 1 cut(s) 1552
MluCI AATT 9 cut(s) 571, 651, 731, 739, 1003, 1092, 1191, 1305, 1355
MlyI GAGTC 1 cut(s) 18
MmeI TCCRAC 1 cut(s) 560
MnlI CCTC 4 cut(s) 436, 663, 981, 1077
Mph1103I ATGCAT 1 cut(s) 41
MseI TTAA 3 cut(s) 714, 923, 1365
MslI CAYNNNNRTG 4 cut(s) 182, 980, 1133, 1367
MspA1I CMGCKG 1 cut(s) 722
MspI CCGG 3 cut(s) 270, 307, 1019
MspR9I CCNGG 3 cut(s) 271, 307, 308
MunI CAATTG 1 cut(s) 1191
Mva1269I GAATGC 1 cut(s) 39
MwoI GCNNNNNNNGC 7 cut(s) 11, 36, 209, 419, 831, 910, 1547
NciI CCSGG 3 cut(s) 271, 307, 308
NcoI CCATGG 1 cut(s) 183
NdeII GATC 5 cut(s) 319, 424, 1086, 1179, 1241
NlaIII CATG 6 cut(s) 39, 187, 607, 729, 979, 1587
NlaIV GGNNCC 3 cut(s) 189, 532, 1211
NmeAIII GCCGAG 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 616
NsiI ATGCAT 1 cut(s) 41
NspV TTCGAA 1 cut(s) 873
PctI GAATGC 1 cut(s) 39
PfeI GAWTC 4 cut(s) 174, 215, 1012, 1149
PkrI GCNGC 8 cut(s) 148, 721, 724, 824, 860, 1189, 1284, 1540
PleI GAGTC 1 cut(s) 18
PpsI GAGTC 1 cut(s) 18
PpuMI RGGWCCY 1 cut(s) 1102
Psp5II RGGWCCY 1 cut(s) 1102
PspN4I GGNNCC 3 cut(s) 189, 532, 1211
PspPI GGNCC 1 cut(s) 1102
PspPPI RGGWCCY 1 cut(s) 1102
PsuI RGATCY 1 cut(s) 1241
PvuII CAGCTG 1 cut(s) 722
RsaI GTAC 2 cut(s) 512, 1523
RsaNI GTAC 2 cut(s) 511, 1522
RseI CAYNNNNRTG 4 cut(s) 182, 980, 1133, 1367
SaqAI TTAA 3 cut(s) 714, 923, 1365
SatI GCNGC 8 cut(s) 147, 720, 723, 823, 859, 1188, 1283, 1539
Sau3AI GATC 5 cut(s) 319, 424, 1086, 1179, 1241
Sau96I GGNCC 1 cut(s) 1102
ScaI AGTACT 1 cut(s) 1523
SchI GAGTC 1 cut(s) 18
ScrFI CCNGG 3 cut(s) 271, 307, 308
SduI GDGCHC 1 cut(s) 1552
SfaNI GCATC 6 cut(s) 101, 320, 708, 1053, 1323, 1550
SfcI CTRYAG 1 cut(s) 1050
SfuI TTCGAA 1 cut(s) 873
SinI GGWCC 1 cut(s) 1102
SmaI CCCGGG 1 cut(s) 308
SmiMI CAYNNNNRTG 4 cut(s) 182, 980, 1133, 1367
SmlI CTYRAG 1 cut(s) 1423
SmoI CTYRAG 1 cut(s) 1423
Sse9I AATT 9 cut(s) 571, 651, 731, 739, 1003, 1092, 1191, 1305, 1355
SsiI CCGC 3 cut(s) 146, 171, 800
SspMI CTAG 7 cut(s) 459, 587, 782, 1238, 1301, 1509, 1514
StyD4I CCNGG 3 cut(s) 269, 305, 306
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 9 cut(s) 157, 164, 437, 535, 1132, 1258, 1324, 1447, 1619
TaqI TCGA 2 cut(s) 873, 953
TaqII GACCGA 1 cut(s) 1156
TasI AATT 9 cut(s) 571, 651, 731, 739, 1003, 1092, 1191, 1305, 1355
TatI WGTACW 1 cut(s) 1521
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 4 cut(s) 174, 215, 1012, 1149
Tru1I TTAA 3 cut(s) 714, 923, 1365
Tru9I TTAA 3 cut(s) 714, 923, 1365
TscAI CASTG 7 cut(s) 442, 553, 939, 1182, 1327, 1405, 1557
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 7 cut(s) 719, 722, 822, 858, 1187, 1282, 1538
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 8 cut(s) 330, 336, 777, 843, 858, 998, 1569, 1638
TspGWI ACGGA 2 cut(s) 131, 332
TspMI CCCGGG 1 cut(s) 306
TspRI CASTG 7 cut(s) 442, 553, 939, 1182, 1327, 1405, 1557
VneI GTGCAC 1 cut(s) 1548
VpaK11BI GGWCC 1 cut(s) 1102
XapI RAATTY 3 cut(s) 651, 1305, 1355
XcmI CCANNNNNNNNNTGG 1 cut(s) 610
XmaI CCCGGG 1 cut(s) 306
XmiI GTMKAC 1 cut(s) 864
XspI CTAG 7 cut(s) 459, 587, 782, 1238, 1301, 1509, 1514
ZrmI AGTACT 1 cut(s) 1523
Zsp2I ATGCAT 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.