MD09G1040700.v1.1

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012381
Physical Location & Seq
Reverse (-)
2607173 .. 2618823
11651 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1040700.v1.1.491

Sequence Viewer

Length: 1581 bp
ATGGCTGGATCAGGAGGAAAAAGACTCGGCTCCTCTTGCTTTCTTCTGATAGTGATTGCTGGGATGGAGAGATTTGCATACAAAGGAGTGGCATCAAATCTAGTGACTTATTTAACCGATGTGGTGAAAATGAGCAACTCGGCAGCGGCCAAGACGGTAAACAGTTGGTGTGGGTTCACATCCATGCTCCCACTCTTAGTTGCACCCCTTGTCGACTCTTACTGGGATAGATATCCCACCATCTTGGTCTCTTCTTTCCTCTATGTTGCAGGGCTTGTGGCATTGACATCAACGGCATTCAAACGGGCAACACCTGCTGCAAACAAAACAAGTTCCAATTCATCATTTCTGTTTTGCTCTCTGTACTTGATTTCTCTAGGTCAAGGTGGATACAACCCATCGCTGCAAGCATTTGGAGCAGACCAACTGGACAGTGAAGAGGAACTTCCTAGCAGCAAAGACGATCAAAAATCAAGCAAAAAGAGAATGTTTTTTCAGTGGTGGTATTTTGGTGTTTGCAGTGGCAGCCTCATGGGTGTCACTCTCATGTCCTACATTCAAGACACTTTCGGTTGGATTATAGGTTTCGCCATCCCCATGATATCGATGGTTATATCGGTTGTGGTGTTTATGTGTGGAAGCCGAATCTATACATATAGAGAGAATGATGAGGCCATGGATTATAAGCCTATTTTCAACATAGTTCAAGTCATCAAGGCAACTGCATTAAAGTTAATGAAATGCAGAATCATATTACCGAATGAAACTAATGTTACCGAGCTAGAGCTTCAAGAGAAACCGCTTTGCCGTCAAAATAATAGCAGCACAGCAAGCTTGGTTGGGAATCCCAAAAGTGGCTTCTACGTGCTTGAAAATGCAAAAGTGCTTTTGAGGCTTCTGCCAATATGGATTATGCTTTTGATGTTTGCAGTGATTTTCCAACAGCCTCCAACATTTTTCACCAAACAAGGCATGACGATGAAGAGGAACATTGGAAGCAACTTCAAGGTTCCACCAGCAACTCTTCAAAGCGCTATCACACTTTCCATAATCCTCCTGATGCCTCTATATGACAAAATAATGATCCCGATTACGCGTCTGGTTACGTCTAATGAAAAGGGTATTAGCGTGATGCAACGGATGGGGATCGGAATGTTCGTTTCGGTCATAGCAATGGTCATTGCAGCGGTTGTGGAAGCAAAAAGGCTCAAGATCAGCAGAGAAATGGAAATGCTGGGATCTCAATCCGAAACTGTACCGTTTAGCATCTTTTGGCTACTTCCACAGTACATTCTTTTAGGCATTTCAGACATTTTCACCGTTGTTGGAATACAAGAGTTCTTTTACTCTGAAGTCCCCAGAAGAATGAGAACAATGGCCTTTGCATTGTACACTAGTGTTTTTGGGGTGGGAAGTTACCTGAGTACCCTTTTGATTGCAACAGTTGAAGCAGTTACAAGTTCAGGAGGAAGGCAGAGCTGGTTCTCCGATGACATGAATGAAGCTAGACTTGATAAATACTACTGGTTTTTTGGCCTCACTAAGTGCATTGAGCTTTGTGTTTTATGTAATTTTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0043207 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

527

Amino Acids

58.64

Weight (kDa)

8.99

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 79 - 494 9.7e-79 POT family
MFS_1 PF07690 167 - 474 2.3e-08 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012381)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01350 AT5G14940
fragaria_vesca FvH4_6g49960
malus_domestica MD09G1040700.v1.1 MD17G1041000.v1.1
prunus_persica Prupe.3G277200_v2.0.a1 Prupe.3G277200_v2.0.a1
pyrus_communis pycom111g03160 pycom17g03740
rosa_chinensis RchiOBHm_Chr2g0170141
rosa_laevigata RLG00000021928
rosa_multiflora Rmu_sc0001605.1_g000022
rosa_roxburghii Rroxscaffold_2G00081560
rosa_rugosa Rorug02G0546100.1
rosa_samantha Rh2BG629700 Rh2CG599200 Rh2DG641600
rosa_wichuraiana Rw0G018340 Rw2G051190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 684
AarI CACCTGC 1 cut(s) 322
Acc36I ACCTGC 1 cut(s) 322
AccI GTMKAC 1 cut(s) 213
AccII CGCG 1 cut(s) 1096
AciI CCGC 3 cut(s) 146, 800, 1187
AclWI GGATC 4 cut(s) 16, 1078, 1154, 1246
AcoI YGGCCR 1 cut(s) 147
AcuI CTGAAG 1 cut(s) 1371
AdeI CACNNNGTG 1 cut(s) 1545
AfaI GTAC 5 cut(s) 365, 1257, 1289, 1391, 1426
AfeI AGCGCT 1 cut(s) 1033
AfiI CCNNNNNNNGG 1 cut(s) 854
AflIII ACRYGT 1 cut(s) 1094
AgsI TTSAA 9 cut(s) 301, 560, 697, 707, 791, 872, 1006, 1028, 1448
AhlI ACTAGT 1 cut(s) 1394
AjuI GAANNNNNNNTTGG 2 cut(s) 842, 874
AluBI AGCT 6 cut(s) 781, 787, 834, 1479, 1505, 1555
AluI AGCT 6 cut(s) 781, 787, 834, 1479, 1505, 1555
Alw26I GTCTC 1 cut(s) 253
AlwI GGATC 4 cut(s) 16, 1078, 1154, 1246
Aor51HI AGCGCT 1 cut(s) 1033
AoxI GGCC 4 cut(s) 147, 672, 1377, 1534
ApeKI GCWGC 7 cut(s) 143, 317, 403, 453, 525, 822, 1184
AspLEI GCGC 1 cut(s) 1034
AsuHPI GGTGA 3 cut(s) 136, 952, 1309
BaeI ACNNNNGTAYC 2 cut(s) 1408, 1441
BbvI GCAGC 7 cut(s) 155, 304, 390, 465, 537, 834, 1196
BccI CCATC 6 cut(s) 58, 248, 406, 599, 601, 1135
BceAI ACGGC 2 cut(s) 309, 792
BciVI GTATCC 1 cut(s) 383
BcoDI GTCTC 1 cut(s) 253
BcuI ACTAGT 1 cut(s) 1394
BfaI CTAG 7 cut(s) 101, 377, 450, 782, 1395, 1506, 1579
BfoI RGCGCY 1 cut(s) 1035
BfuAI ACCTGC 1 cut(s) 322
BfuI GTATCC 1 cut(s) 383
BisI GCNGC 8 cut(s) 144, 147, 318, 404, 454, 526, 823, 1185
BlsI GCNGC 8 cut(s) 145, 148, 319, 405, 455, 527, 824, 1186
BmiI GGNNCC 2 cut(s) 31, 1011
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 4 cut(s) 101, 1050, 1122, 1275
BmuI ACTGGG 1 cut(s) 232
BpuEI CTTGAG 1 cut(s) 1193
Bsa29I ATCGAT 1 cut(s) 605
BsaAI YACGTR 1 cut(s) 865
BsaBI GATNNNNATC 3 cut(s) 231, 1145, 1243
BsaI GGTCTC 1 cut(s) 253
BsaJI CCNNGG 1 cut(s) 675
Bsc4I CCNNNNNNNGG 1 cut(s) 854
Bse1I ACTGG 3 cut(s) 227, 432, 1529
Bse3DI GCAATG 2 cut(s) 1179, 1179
Bse8I GATNNNNATC 3 cut(s) 231, 1145, 1243
BseCI ATCGAT 1 cut(s) 605
BseDI CCNNGG 1 cut(s) 675
BseGI GGATG 4 cut(s) 69, 179, 591, 1146
BseJI GATNNNNATC 3 cut(s) 231, 1145, 1243
BseLI CCNNNNNNNGG 1 cut(s) 854
BseMI GCAATG 2 cut(s) 1179, 1179
BseMII CTCAG 1 cut(s) 1412
BseNI ACTGG 3 cut(s) 227, 432, 1529
BseRI GAGGAG 1 cut(s) 22
BseXI GCAGC 7 cut(s) 155, 304, 390, 465, 537, 834, 1196
BseYI CCCAGC 2 cut(s) 59, 1234
Bsh1236I CGCG 1 cut(s) 1096
BshFI GGCC 4 cut(s) 149, 674, 1379, 1536
BshVI ATCGAT 1 cut(s) 605
BslFI GGGAC 1 cut(s) 1340
BslI CCNNNNNNNGG 1 cut(s) 854
BsmAI GTCTC 1 cut(s) 253
BsmFI GGGAC 1 cut(s) 1340
BsmI GAATGC 1 cut(s) 296
BsnI GGCC 4 cut(s) 149, 674, 1379, 1536
Bso31I GGTCTC 1 cut(s) 253
Bsp1407I TGTACA 1 cut(s) 1389
Bsp143I GATC 6 cut(s) 8, 463, 1083, 1146, 1212, 1238
Bsp19I CCATGG 1 cut(s) 675
BspACI CCGC 3 cut(s) 146, 800, 1187
BspANI GGCC 4 cut(s) 149, 674, 1379, 1536
BspCNI CTCAG 1 cut(s) 1413
BspDI ATCGAT 1 cut(s) 605
BspFNI CGCG 1 cut(s) 1096
BspLI GGNNCC 2 cut(s) 31, 1011
BspMI ACCTGC 1 cut(s) 322
BspPI GGATC 4 cut(s) 16, 1078, 1154, 1246
BspTNI GGTCTC 1 cut(s) 253
BsrDI GCAATG 2 cut(s) 1179, 1179
BsrGI TGTACA 1 cut(s) 1389
BsrI ACTGG 3 cut(s) 227, 432, 1529
BssECI CCNNGG 1 cut(s) 675
BssMI GATC 6 cut(s) 8, 463, 1083, 1146, 1212, 1238
BssT1I CCWWGG 1 cut(s) 675
Bst4CI ACNGT 8 cut(s) 157, 164, 434, 1255, 1260, 1287, 1321, 1444
Bst6I CTCTTC 4 cut(s) 256, 432, 977, 1029
BstAPI GCANNNNNTGC 1 cut(s) 314
BstAUI TGTACA 1 cut(s) 1389
BstBAI YACGTR 1 cut(s) 865
BstC8I GCNNGC 2 cut(s) 408, 832
BstDEI CTNAG 3 cut(s) 196, 1421, 1542
BstDSI CCRYGG 1 cut(s) 675
BstF5I GGATG 4 cut(s) 69, 179, 591, 1146
BstFNI CGCG 1 cut(s) 1096
BstH2I RGCGCY 1 cut(s) 1035
BstHHI GCGC 1 cut(s) 1034
BstKTI GATC 6 cut(s) 11, 466, 1086, 1149, 1215, 1241
BstMAI GTCTC 1 cut(s) 253
BstMBI GATC 6 cut(s) 8, 463, 1083, 1146, 1212, 1238
BstMWI GCNNNNNNNGC 6 cut(s) 36, 314, 416, 525, 831, 892
BstUI CGCG 1 cut(s) 1096
BstV1I GCAGC 7 cut(s) 155, 304, 390, 465, 537, 834, 1196
BstX2I RGATCY 1 cut(s) 1238
BstXI CCANNNNNNTGG 1 cut(s) 244
BstYI RGATCY 1 cut(s) 1238
Bsu15I ATCGAT 1 cut(s) 605
BsuI GTATCC 1 cut(s) 383
BsuRI GGCC 4 cut(s) 149, 674, 1379, 1536
BsuTUI ATCGAT 1 cut(s) 605
BtgI CCRYGG 1 cut(s) 675
BtgZI GCGATG 1 cut(s) 384
BtsCI GGATG 4 cut(s) 69, 179, 591, 1146
BtsI GCAGTG 2 cut(s) 526, 936
BtsIMutI CAGTG 4 cut(s) 439, 503, 526, 936
BveI ACCTGC 1 cut(s) 322
Cac8I GCNNGC 2 cut(s) 408, 832
CfoI GCGC 1 cut(s) 1034
ClaI ATCGAT 1 cut(s) 605
CseI GACGC 1 cut(s) 1085
Csp6I GTAC 5 cut(s) 364, 1256, 1288, 1390, 1425
CviAII CATG 7 cut(s) 184, 532, 547, 598, 676, 973, 1495
CviQI GTAC 5 cut(s) 364, 1256, 1288, 1390, 1425
DdeI CTNAG 3 cut(s) 196, 1421, 1542
DpnI GATC 6 cut(s) 10, 465, 1085, 1148, 1214, 1240
DpnII GATC 6 cut(s) 8, 463, 1083, 1146, 1212, 1238
DraIII CACNNNGTG 1 cut(s) 1545
EaeI YGGCCR 1 cut(s) 147
Eam1104I CTCTTC 4 cut(s) 256, 432, 977, 1029
EarI CTCTTC 4 cut(s) 256, 432, 977, 1029
Eco130I CCWWGG 1 cut(s) 675
Eco31I GGTCTC 1 cut(s) 253
Eco32I GATATC 2 cut(s) 233, 603
Eco47III AGCGCT 1 cut(s) 1033
Eco57I CTGAAG 1 cut(s) 1371
EcoRV GATATC 2 cut(s) 233, 603
EcoT14I CCWWGG 1 cut(s) 675
ErhI CCWWGG 1 cut(s) 675
FaeI CATG 7 cut(s) 187, 535, 550, 601, 679, 976, 1498
FalI AAGNNNNNCTT 4 cut(s) 429, 461, 1494, 1526
FaqI GGGAC 1 cut(s) 1340
FatI CATG 7 cut(s) 183, 531, 546, 597, 675, 972, 1494
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 8 cut(s) 144, 147, 318, 404, 454, 526, 823, 1185
FokI GGATG 4 cut(s) 76, 166, 578, 1153
Fsp4HI GCNGC 8 cut(s) 144, 147, 318, 404, 454, 526, 823, 1185
FspBI CTAG 7 cut(s) 101, 377, 450, 782, 1395, 1506, 1579
GlaI GCGC 1 cut(s) 1033
GluI GCNGC 8 cut(s) 144, 147, 318, 404, 454, 526, 823, 1185
GsaI CCCAGC 2 cut(s) 63, 1238
HaeII RGCGCY 1 cut(s) 1035
HaeIII GGCC 4 cut(s) 149, 674, 1379, 1536
HgaI GACGC 1 cut(s) 1085
HhaI GCGC 1 cut(s) 1034
Hin1II CATG 7 cut(s) 187, 535, 550, 601, 679, 976, 1498
Hin6I GCGC 1 cut(s) 1032
HinP1I GCGC 1 cut(s) 1032
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 1 cut(s) 832
HinfI GANTC 5 cut(s) 24, 215, 645, 747, 844
HphI GGTGA 3 cut(s) 136, 952, 1309
Hpy166II GTNNAC 4 cut(s) 160, 177, 214, 1392
Hpy188I TCNGA 6 cut(s) 48, 1151, 1249, 1309, 1351, 1489
Hpy188III TCNNGA 7 cut(s) 12, 560, 791, 1057, 1087, 1210, 1464
Hpy8I GTNNAC 4 cut(s) 160, 177, 214, 1392
HpyAV CCTTC 1 cut(s) 1464
HpyCH4III ACNGT 8 cut(s) 157, 164, 434, 1255, 1260, 1287, 1321, 1444
HpyCH4IV ACGT 2 cut(s) 864, 1106
HpyF10VI GCNNNNNNNGC 6 cut(s) 36, 314, 416, 525, 831, 892
HpyF3I CTNAG 3 cut(s) 196, 1421, 1542
HpySE526I ACGT 2 cut(s) 864, 1106
Hsp92II CATG 7 cut(s) 187, 535, 550, 601, 679, 976, 1498
HspAI GCGC 1 cut(s) 1032
Kzo9I GATC 6 cut(s) 8, 463, 1083, 1146, 1212, 1238
LmnI GCTCC 3 cut(s) 35, 192, 416
Lsp1109I GCAGC 7 cut(s) 155, 304, 390, 465, 537, 834, 1196
LweI GCATC 4 cut(s) 101, 1050, 1122, 1275
MaeI CTAG 7 cut(s) 101, 377, 450, 782, 1395, 1506, 1579
MaeII ACGT 2 cut(s) 864, 1106
MaeIII GTNAC 6 cut(s) 103, 538, 772, 1102, 1415, 1453
MalI GATC 6 cut(s) 10, 465, 1085, 1148, 1214, 1240
MboI GATC 6 cut(s) 8, 463, 1083, 1146, 1212, 1238
MboII GAAGA 6 cut(s) 35, 243, 449, 994, 1016, 1374
MflI RGATCY 1 cut(s) 1238
MluCI AATT 2 cut(s) 337, 1570
MluI ACGCGT 1 cut(s) 1094
MlyI GAGTC 2 cut(s) 18, 209
MmeI TCCRAC 4 cut(s) 554, 964, 974, 1306
MseI TTAA 3 cut(s) 113, 728, 734
MslI CAYNNNNRTG 5 cut(s) 182, 545, 596, 977, 1172
MspA1I CMGCKG 2 cut(s) 146, 1187
Mva1269I GAATGC 1 cut(s) 296
MvnI CGCG 1 cut(s) 1096
MwoI GCNNNNNNNGC 6 cut(s) 36, 314, 416, 525, 831, 892
NcoI CCATGG 1 cut(s) 675
NdeII GATC 6 cut(s) 8, 463, 1083, 1146, 1212, 1238
NlaIII CATG 7 cut(s) 187, 535, 550, 601, 679, 976, 1498
NlaIV GGNNCC 2 cut(s) 31, 1011
NmeAIII GCCGAG 2 cut(s) 6, 119
NmuCI GTSAC 2 cut(s) 103, 538
PaqCI CACCTGC 1 cut(s) 322
PcsI WCGNNNNNNNCGW 1 cut(s) 1155
PctI GAATGC 1 cut(s) 296
PfeI GAWTC 3 cut(s) 645, 747, 844
PkrI GCNGC 8 cut(s) 145, 148, 319, 405, 455, 527, 824, 1186
PleI GAGTC 2 cut(s) 18, 209
PpsI GAGTC 2 cut(s) 18, 209
Ppu21I YACGTR 1 cut(s) 865
PsiI TTATAA 1 cut(s) 684
PspFI CCCAGC 2 cut(s) 59, 1234
PspN4I GGNNCC 2 cut(s) 31, 1011
PsuI RGATCY 1 cut(s) 1238
RsaI GTAC 5 cut(s) 365, 1257, 1289, 1391, 1426
RsaNI GTAC 5 cut(s) 364, 1256, 1288, 1390, 1425
RseI CAYNNNNRTG 5 cut(s) 182, 545, 596, 977, 1172
SalI GTCGAC 1 cut(s) 212
SaqAI TTAA 3 cut(s) 113, 728, 734
SatI GCNGC 8 cut(s) 144, 147, 318, 404, 454, 526, 823, 1185
Sau3AI GATC 6 cut(s) 8, 463, 1083, 1146, 1212, 1238
SchI GAGTC 2 cut(s) 18, 209
SfaNI GCATC 4 cut(s) 101, 1050, 1122, 1275
SmiMI CAYNNNNRTG 5 cut(s) 182, 545, 596, 977, 1172
SmlI CTYRAG 1 cut(s) 1208
SmoI CTYRAG 1 cut(s) 1208
SpeI ACTAGT 1 cut(s) 1394
Sse9I AATT 2 cut(s) 337, 1570
SsiI CCGC 3 cut(s) 146, 800, 1187
SspMI CTAG 7 cut(s) 101, 377, 450, 782, 1395, 1506, 1579
StyI CCWWGG 1 cut(s) 675
TaaI ACNGT 8 cut(s) 157, 164, 434, 1255, 1260, 1287, 1321, 1444
TaiI ACGT 2 cut(s) 867, 1109
TaqI TCGA 2 cut(s) 213, 605
TaqII GACCGA 1 cut(s) 1153
TasI AATT 2 cut(s) 337, 1570
TatI WGTACW 3 cut(s) 363, 1287, 1389
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 3 cut(s) 645, 747, 844
Tru1I TTAA 3 cut(s) 113, 728, 734
Tru9I TTAA 3 cut(s) 113, 728, 734
TscAI CASTG 4 cut(s) 439, 503, 526, 936
TseFI GTSAC 2 cut(s) 103, 538
TseI GCWGC 7 cut(s) 143, 317, 403, 453, 525, 822, 1184
Tsp45I GTSAC 2 cut(s) 103, 538
TspDTI ATGAA 7 cut(s) 330, 752, 777, 995, 1128, 1511, 1515
TspGWI ACGGA 1 cut(s) 1153
TspRI CASTG 4 cut(s) 439, 503, 526, 936
XcmI CCANNNNNNNNNTGG 1 cut(s) 604
XmiI GTMKAC 1 cut(s) 213
XspI CTAG 7 cut(s) 101, 377, 450, 782, 1395, 1506, 1579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.