Rroxscaffold_2G00081560

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012381
Physical Location & Seq
Reverse (-)
4630921 .. 4634965
4045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00081560.1

Sequence Viewer

Length: 1644 bp
ATGGCTGGTTCAACAAGAAAGAGACTCGGCAACTCATGCATTCTTCTCATAGTAATTGCTGGTATGGAAAGATTTGCATACAAAGGAGTGGCATCCAATCTGATAACTTATCTGACCGATGTAGTAAAGATGAGCAACTCTTCTGCGGCGAAGACGGTGAACAGGTGGTGCGGATTCACATCCATGGTTCCACTCTTGATTGCACCCCTTGCTGATTCTTACTGGGATAGATATTACACCATTGTAGCCTCTTGTTTTATCTATGTTGCCGGGCTTGTGGCATTGACATCAACAGCACTAATGTCCCGGGCATCGTCCGATCAAGCAAACAAAACAAGTTCATCTTCATTTCTGTTTTGCTCATTGTATTTGATTTCTCTTGGTCAAGGTGGGTACAACCCATCTTTGCAAGCTTTTGGAGCAGATCAACTTGATAGTGAAGAGGAAGAGTTGCCTAGTAGCAAAGATGACAAAAAATCCAACAAAAAAAGTTTGTTCTTTCAATGGTGGTACTTTGGTATTTGTAGTGGCACCGTCTTGGGTATCAGTGTTATGTCCTACATTCAAGATACTTTTGGTTGGATACTAGGTTTTGCCATCCCCATGATTTCGATGGTCACATCAGTCATAGTTTTCTCGTGTGGAAGCCCAATTTATACATATAGACAGAGAAATGAGACCATGGGTAATAAGCCAGTTGTGAGCATGCTTCAAGTTATTAAGGCAGCTGCATTGAAATTACTGAATTCCAAAGTCAAGTTACCCGATGAAAATGATGTTACTGAGCTAGAGCTTCAAGGGAAACCGCTCTGTCGTCAGAACTTAAGCAGCAATGAAGGCTTGAACAATGAGCATCCCAAAAGAAGCATCCACATTTTCGAAAATGCAAAAGTTATTCTTAGGCTTTTGCCCATATGGCTAATGCTTTTAATGTTTGCAGTGATTTTCCAGCAACCTTCGACATTCTTCACCAAACAAGGCATGACAATGAAGAGGAATATCGGAAGCAATTTTCTGATTCCACCCGCAACACTACAGAGTGCTATTGCACTTTCTATAGTTGTGTTGATGCCTCTTTATGACAAAGTTCTGATCCCAATTACAAAGGTCCTGACTTGTAACGAAAAGGGTATCACCGTAATGCAAAGGATGGGGATTGGGATGTTCACTTCGGTCATAGCACTGATCATCGCAGCACTTGTAGAGACAAGAAGGCTCCATATCAGCAGAAATATGGAAATGCTAGGATCTCAATCTGAGACGGTTCCATTAAGCATATTCTGGTTGCTGCCACAATACATTCTTCTGGGCATTTCGGACATTTTCACTGTCGTTGGGATGCAAGAGTTCTTTTACTCTGAATTTCCGATTAGAATGAGAACAATGGCCTTTGCACTATACACCAGTGTTTTTGGGGTTGGGAGCTACTTGAGTGCCCTTTTGATCTCAACAGTTGAAGGCATCACAAGTTCAAATGGAAGTAAGAGCTGGTTCTGTGATGATATGACTGAAGCAAGGCTAGACAAGTACTACTGGTTCTTAGCAGCAGCAAGTGCACTGAGCTTTCTGTTTTTTGTGATTATTTCATCATTTTACACAAGTAAGAGGGATTTGGAGTTGGACAGTAATGAAAGTTGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0043207 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

547

Amino Acids

60.93

Weight (kDa)

8.74

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 79 - 497 5.4e-73 POT family
MFS_1 PF07690 169 - 477 7.2e-07 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012381)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01350 AT5G14940
fragaria_vesca FvH4_6g49960
malus_domestica MD09G1040700.v1.1 MD17G1041000.v1.1
prunus_persica Prupe.3G277200_v2.0.a1 Prupe.3G277200_v2.0.a1
pyrus_communis pycom111g03160 pycom17g03740
rosa_chinensis RchiOBHm_Chr2g0170141
rosa_laevigata RLG00000021928
rosa_multiflora Rmu_sc0001605.1_g000022
rosa_roxburghii Rroxscaffold_2G00081560
rosa_rugosa Rorug02G0546100.1
rosa_samantha Rh2BG629700 Rh2CG599200 Rh2DG641600
rosa_wichuraiana Rw0G018340 Rw2G051190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 530
AccBSI CCGCTC 1 cut(s) 808
AciI CCGC 4 cut(s) 146, 171, 806, 1026
AclWI GGATC 2 cut(s) 1087, 1255
AcsI RAATTY 2 cut(s) 745, 1361
AcuI CTGAAG 1 cut(s) 1530
AfaI GTAC 3 cut(s) 395, 512, 1529
AflII CTTAAG 1 cut(s) 823
AgsI TTSAA 9 cut(s) 12, 503, 566, 713, 736, 797, 844, 1457, 1473
AluBI AGCT 7 cut(s) 413, 728, 787, 793, 1425, 1488, 1563
AluI AGCT 7 cut(s) 413, 728, 787, 793, 1425, 1488, 1563
Alw21I GWGCWC 1 cut(s) 1558
Alw26I GTCTC 4 cut(s) 16, 671, 1199, 1253
Alw44I GTGCAC 1 cut(s) 1554
AlwI GGATC 2 cut(s) 1087, 1255
Ama87I CYCGRG 1 cut(s) 306
AoxI GGCC 1 cut(s) 1386
ApaLI GTGCAC 1 cut(s) 1554
ApeKI GCWGC 7 cut(s) 725, 728, 828, 1193, 1288, 1544, 1547
ApoI RAATTY 2 cut(s) 745, 1361
AspS9I GGNCC 1 cut(s) 1108
AsuC2I CCSGG 3 cut(s) 271, 307, 308
AsuHPI GGTGA 3 cut(s) 169, 961, 1126
AsuII TTCGAA 1 cut(s) 879
AvaI CYCGRG 1 cut(s) 306
AvaII GGWCC 1 cut(s) 1108
BaeGI GKGCMC 2 cut(s) 1438, 1558
BaeI ACNNNNGTAYC 2 cut(s) 502, 535
BanI GGYRCC 1 cut(s) 530
BauI CACGAG 1 cut(s) 637
BbsI GAAGAC 1 cut(s) 158
Bbv12I GWGCWC 1 cut(s) 1558
BbvI GCAGC 7 cut(s) 715, 737, 840, 1205, 1275, 1556, 1559
BccI CCATC 4 cut(s) 409, 605, 607, 1144
BciVI GTATCC 1 cut(s) 576
BclI TGATCA 1 cut(s) 1185
BcnI CCSGG 3 cut(s) 271, 307, 308
BcoDI GTCTC 4 cut(s) 16, 671, 1199, 1253
BfaI CTAG 5 cut(s) 456, 587, 788, 1244, 1520
BfmI CTRYAG 2 cut(s) 1034, 1056
BfrI CTTAAG 1 cut(s) 823
BfuI GTATCC 1 cut(s) 576
BglI GCCNNNNNGGC 1 cut(s) 916
BisI GCNGC 8 cut(s) 147, 726, 729, 829, 1194, 1289, 1545, 1548
BlsI GCNGC 8 cut(s) 148, 727, 730, 830, 1195, 1290, 1546, 1549
BmcAI AGTACT 1 cut(s) 1529
Bme1390I CCNGG 3 cut(s) 271, 307, 308
Bme18I GGWCC 1 cut(s) 1108
BmeT110I CYCGRG 1 cut(s) 306
BmgT120I GGNCC 1 cut(s) 1108
BmiI GGNNCC 4 cut(s) 189, 532, 1217, 1266
BmrFI CCNGG 3 cut(s) 271, 307, 308
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 7 cut(s) 101, 320, 862, 876, 1059, 1329, 1470
BmuI ACTGGG 1 cut(s) 232
BpiI GAAGAC 1 cut(s) 158
Bpu14I TTCGAA 1 cut(s) 879
BpuEI CTTGAG 1 cut(s) 1450
BpuMI CCSGG 3 cut(s) 271, 307, 308
BsaBI GATNNNNATC 2 cut(s) 178, 1252
BsaI GGTCTC 1 cut(s) 671
BsaJI CCNNGG 3 cut(s) 183, 306, 681
Bse1I ACTGG 4 cut(s) 227, 695, 1404, 1538
Bse3DI GCAATG 1 cut(s) 838
Bse8I GATNNNNATC 2 cut(s) 178, 1252
BseDI CCNNGG 3 cut(s) 183, 306, 681
BseGI GGATG 8 cut(s) 92, 179, 597, 853, 867, 1155, 1167, 1344
BseJI GATNNNNATC 2 cut(s) 178, 1252
BseMI GCAATG 1 cut(s) 838
BseMII CTCAG 3 cut(s) 774, 1248, 1550
BseNI ACTGG 4 cut(s) 227, 695, 1404, 1538
BseSI GKGCMC 2 cut(s) 1438, 1558
BseXI GCAGC 7 cut(s) 715, 737, 840, 1205, 1275, 1556, 1559
BshFI GGCC 1 cut(s) 1388
BshNI GGYRCC 1 cut(s) 530
BsiHKAI GWGCWC 1 cut(s) 1558
BsiHKCI CYCGRG 1 cut(s) 306
BsiSI CCGG 2 cut(s) 270, 307
BslFI GGGAC 1 cut(s) 289
BsmAI GTCTC 4 cut(s) 16, 671, 1199, 1253
BsmBI CGTCTC 1 cut(s) 1253
BsmFI GGGAC 1 cut(s) 289
BsmI GAATGC 1 cut(s) 39
BsnI GGCC 1 cut(s) 1388
Bso31I GGTCTC 1 cut(s) 671
BsoBI CYCGRG 1 cut(s) 306
Bsp119I TTCGAA 1 cut(s) 879
Bsp1286I GDGCHC 2 cut(s) 1438, 1558
Bsp143I GATC 6 cut(s) 319, 424, 1092, 1185, 1247, 1443
Bsp19I CCATGG 2 cut(s) 183, 681
BspACI CCGC 4 cut(s) 146, 171, 806, 1026
BspANI GGCC 1 cut(s) 1388
BspCNI CTCAG 3 cut(s) 775, 1249, 1551
BspLI GGNNCC 4 cut(s) 189, 532, 1217, 1266
BspPI GGATC 2 cut(s) 1087, 1255
BspT104I TTCGAA 1 cut(s) 879
BspT107I GGYRCC 1 cut(s) 530
BspTI CTTAAG 1 cut(s) 823
BspTNI GGTCTC 1 cut(s) 671
BsrBI CCGCTC 1 cut(s) 808
BsrDI GCAATG 1 cut(s) 838
BsrI ACTGG 4 cut(s) 227, 695, 1404, 1538
BssECI CCNNGG 3 cut(s) 183, 306, 681
BssMI GATC 6 cut(s) 319, 424, 1092, 1185, 1247, 1443
BssSI CACGAG 1 cut(s) 637
BssT1I CCWWGG 2 cut(s) 183, 681
Bst2BI CACGAG 1 cut(s) 637
Bst4CI ACNGT 7 cut(s) 157, 535, 1138, 1264, 1330, 1453, 1625
Bst6I CTCTTC 4 cut(s) 145, 435, 441, 986
BstAFI CTTAAG 1 cut(s) 823
BstAPI GCANNNNNTGC 3 cut(s) 36, 209, 1553
BstBI TTCGAA 1 cut(s) 879
BstC8I GCNNGC 2 cut(s) 411, 707
BstDEI CTNAG 5 cut(s) 783, 899, 1257, 1540, 1559
BstDSI CCRYGG 2 cut(s) 183, 681
BstF5I GGATG 8 cut(s) 92, 179, 597, 853, 867, 1155, 1167, 1344
BstKTI GATC 6 cut(s) 322, 427, 1095, 1188, 1250, 1446
BstMAI GTCTC 4 cut(s) 16, 671, 1199, 1253
BstMBI GATC 6 cut(s) 319, 424, 1092, 1185, 1247, 1443
BstMWI GCNNNNNNNGC 6 cut(s) 36, 209, 419, 837, 916, 1553
BstNSI RCATGY 1 cut(s) 709
BstSCI CCNGG 3 cut(s) 269, 305, 306
BstSFI CTRYAG 2 cut(s) 1034, 1056
BstSLI GKGCMC 2 cut(s) 1438, 1558
BstV1I GCAGC 7 cut(s) 715, 737, 840, 1205, 1275, 1556, 1559
BstV2I GAAGAC 1 cut(s) 158
BstX2I RGATCY 1 cut(s) 1247
BstYI RGATCY 1 cut(s) 1247
BsuI GTATCC 1 cut(s) 576
BsuRI GGCC 1 cut(s) 1388
BtgI CCRYGG 2 cut(s) 183, 681
BtgZI GCGATG 1 cut(s) 1174
BtsCI GGATG 8 cut(s) 92, 179, 597, 853, 867, 1155, 1167, 1344
BtsI GCAGTG 1 cut(s) 945
BtsIMutI CAGTG 6 cut(s) 553, 945, 1181, 1326, 1411, 1556
Cac8I GCNNGC 2 cut(s) 411, 707
Cfr13I GGNCC 1 cut(s) 1108
Cfr9I CCCGGG 1 cut(s) 306
Csp6I GTAC 3 cut(s) 394, 511, 1528
CviAII CATG 6 cut(s) 36, 184, 604, 682, 706, 982
CviQI GTAC 3 cut(s) 394, 511, 1528
DdeI CTNAG 5 cut(s) 783, 899, 1257, 1540, 1559
DpnI GATC 6 cut(s) 321, 426, 1094, 1187, 1249, 1445
DpnII GATC 6 cut(s) 319, 424, 1092, 1185, 1247, 1443
Eam1104I CTCTTC 4 cut(s) 145, 435, 441, 986
EarI CTCTTC 4 cut(s) 145, 435, 441, 986
Eco130I CCWWGG 2 cut(s) 183, 681
Eco31I GGTCTC 1 cut(s) 671
Eco47I GGWCC 1 cut(s) 1108
Eco57I CTGAAG 1 cut(s) 1530
Eco88I CYCGRG 1 cut(s) 306
EcoO109I RGGNCCY 1 cut(s) 1108
EcoRI GAATTC 1 cut(s) 745
EcoT14I CCWWGG 2 cut(s) 183, 681
EcoT22I ATGCAT 1 cut(s) 41
ErhI CCWWGG 2 cut(s) 183, 681
Esp3I CGTCTC 1 cut(s) 1253
FaeI CATG 6 cut(s) 39, 187, 607, 685, 709, 985
FalI AAGNNNNNCTT 4 cut(s) 328, 360, 882, 914
FaqI GGGAC 1 cut(s) 289
FatI CATG 6 cut(s) 35, 183, 603, 681, 705, 981
FauI CCCGC 1 cut(s) 1033
FauNDI CATATG 1 cut(s) 914
FbaI TGATCA 1 cut(s) 1185
Fnu4HI GCNGC 8 cut(s) 147, 726, 729, 829, 1194, 1289, 1545, 1548
FokI GGATG 8 cut(s) 79, 166, 584, 840, 854, 1162, 1174, 1351
Fsp4HI GCNGC 8 cut(s) 147, 726, 729, 829, 1194, 1289, 1545, 1548
FspBI CTAG 5 cut(s) 456, 587, 788, 1244, 1520
GluI GCNGC 8 cut(s) 147, 726, 729, 829, 1194, 1289, 1545, 1548
HaeIII GGCC 1 cut(s) 1388
HapII CCGG 2 cut(s) 270, 307
Hin1II CATG 6 cut(s) 39, 187, 607, 685, 709, 985
HindIII AAGCTT 1 cut(s) 411
HinfI GANTC 4 cut(s) 24, 174, 215, 1018
HpaII CCGG 2 cut(s) 270, 307
HphI GGTGA 3 cut(s) 169, 961, 1126
Hpy166II GTNNAC 3 cut(s) 160, 1167, 1556
Hpy188III TCNNGA 3 cut(s) 196, 566, 1111
Hpy8I GTNNAC 3 cut(s) 160, 1167, 1556
HpyAV CCTTC 4 cut(s) 830, 966, 1206, 1451
HpyCH4III ACNGT 7 cut(s) 157, 535, 1138, 1264, 1330, 1453, 1625
HpyF10VI GCNNNNNNNGC 6 cut(s) 36, 209, 419, 837, 916, 1553
HpyF3I CTNAG 5 cut(s) 783, 899, 1257, 1540, 1559
Hsp92II CATG 6 cut(s) 39, 187, 607, 685, 709, 985
Ksp22I TGATCA 1 cut(s) 1185
Kzo9I GATC 6 cut(s) 319, 424, 1092, 1185, 1247, 1443
LmnI GCTCC 3 cut(s) 419, 1221, 1422
Lsp1109I GCAGC 7 cut(s) 715, 737, 840, 1205, 1275, 1556, 1559
LweI GCATC 7 cut(s) 101, 320, 862, 876, 1059, 1329, 1470
MaeI CTAG 5 cut(s) 456, 587, 788, 1244, 1520
MaeIII GTNAC 4 cut(s) 616, 759, 778, 1118
MalI GATC 6 cut(s) 321, 426, 1094, 1187, 1249, 1445
MbiI CCGCTC 1 cut(s) 808
MboI GATC 6 cut(s) 319, 424, 1092, 1185, 1247, 1443
MboII GAAGA 9 cut(s) 35, 132, 163, 336, 452, 458, 958, 1003, 1295
MflI RGATCY 1 cut(s) 1247
MhlI GDGCHC 2 cut(s) 1438, 1558
MluCI AATT 7 cut(s) 54, 651, 737, 745, 1009, 1098, 1361
MlyI GAGTC 1 cut(s) 18
MmeI TCCRAC 3 cut(s) 504, 560, 1599
MnlI CCTC 5 cut(s) 259, 436, 987, 1083, 1599
Mph1103I ATGCAT 1 cut(s) 41
MseI TTAA 4 cut(s) 720, 824, 929, 1271
MslI CAYNNNNRTG 4 cut(s) 182, 602, 986, 1139
MspA1I CMGCKG 1 cut(s) 728
MspCI CTTAAG 1 cut(s) 823
MspI CCGG 2 cut(s) 270, 307
MspR9I CCNGG 3 cut(s) 271, 307, 308
Mva1269I GAATGC 1 cut(s) 39
MwoI GCNNNNNNNGC 6 cut(s) 36, 209, 419, 837, 916, 1553
NciI CCSGG 3 cut(s) 271, 307, 308
NcoI CCATGG 2 cut(s) 183, 681
NdeI CATATG 1 cut(s) 914
NdeII GATC 6 cut(s) 319, 424, 1092, 1185, 1247, 1443
NlaIII CATG 6 cut(s) 39, 187, 607, 685, 709, 985
NlaIV GGNNCC 4 cut(s) 189, 532, 1217, 1266
NmeAIII GCCGAG 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 616
NsiI ATGCAT 1 cut(s) 41
NspI RCATGY 1 cut(s) 709
NspV TTCGAA 1 cut(s) 879
PaeI GCATGC 1 cut(s) 709
PctI GAATGC 1 cut(s) 39
PfeI GAWTC 3 cut(s) 174, 215, 1018
PkrI GCNGC 8 cut(s) 148, 727, 730, 830, 1195, 1290, 1546, 1549
PleI GAGTC 1 cut(s) 18
PpsI GAGTC 1 cut(s) 18
PpuMI RGGWCCY 1 cut(s) 1108
Psp5II RGGWCCY 1 cut(s) 1108
PspN4I GGNNCC 4 cut(s) 189, 532, 1217, 1266
PspPI GGNCC 1 cut(s) 1108
PspPPI RGGWCCY 1 cut(s) 1108
PsrI GAACNNNNNNTAC 2 cut(s) 1520, 1552
PsuI RGATCY 1 cut(s) 1247
PvuII CAGCTG 1 cut(s) 728
RsaI GTAC 3 cut(s) 395, 512, 1529
RsaNI GTAC 3 cut(s) 394, 511, 1528
RseI CAYNNNNRTG 4 cut(s) 182, 602, 986, 1139
SaqAI TTAA 4 cut(s) 720, 824, 929, 1271
SatI GCNGC 8 cut(s) 147, 726, 729, 829, 1194, 1289, 1545, 1548
Sau3AI GATC 6 cut(s) 319, 424, 1092, 1185, 1247, 1443
Sau96I GGNCC 1 cut(s) 1108
ScaI AGTACT 1 cut(s) 1529
SchI GAGTC 1 cut(s) 18
ScrFI CCNGG 3 cut(s) 271, 307, 308
SduI GDGCHC 2 cut(s) 1438, 1558
SfaNI GCATC 7 cut(s) 101, 320, 862, 876, 1059, 1329, 1470
SfcI CTRYAG 2 cut(s) 1034, 1056
SfuI TTCGAA 1 cut(s) 879
SinI GGWCC 1 cut(s) 1108
SmaI CCCGGG 1 cut(s) 308
SmiMI CAYNNNNRTG 4 cut(s) 182, 602, 986, 1139
SmlI CTYRAG 2 cut(s) 823, 1429
SmoI CTYRAG 2 cut(s) 823, 1429
SphI GCATGC 1 cut(s) 709
Sse9I AATT 7 cut(s) 54, 651, 737, 745, 1009, 1098, 1361
SsiI CCGC 4 cut(s) 146, 171, 806, 1026
SspMI CTAG 5 cut(s) 456, 587, 788, 1244, 1520
StyD4I CCNGG 3 cut(s) 269, 305, 306
StyI CCWWGG 2 cut(s) 183, 681
TaaI ACNGT 7 cut(s) 157, 535, 1138, 1264, 1330, 1453, 1625
TaqI TCGA 3 cut(s) 611, 879, 959
TaqII GACCGA 2 cut(s) 131, 1162
TasI AATT 7 cut(s) 54, 651, 737, 745, 1009, 1098, 1361
TatI WGTACW 1 cut(s) 1527
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 3 cut(s) 174, 215, 1018
Tru1I TTAA 4 cut(s) 720, 824, 929, 1271
Tru9I TTAA 4 cut(s) 720, 824, 929, 1271
TscAI CASTG 6 cut(s) 553, 945, 1188, 1333, 1411, 1563
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 7 cut(s) 725, 728, 828, 1193, 1288, 1544, 1547
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 7 cut(s) 330, 336, 783, 849, 1004, 1575, 1644
TspMI CCCGGG 1 cut(s) 306
TspRI CASTG 6 cut(s) 553, 945, 1188, 1333, 1411, 1563
Vha464I CTTAAG 1 cut(s) 823
VneI GTGCAC 1 cut(s) 1554
VpaK11BI GGWCC 1 cut(s) 1108
XapI RAATTY 2 cut(s) 745, 1361
XceI RCATGY 1 cut(s) 709
XcmI CCANNNNNNNNNTGG 1 cut(s) 610
XmaI CCCGGG 1 cut(s) 306
XspI CTAG 5 cut(s) 456, 587, 788, 1244, 1520
ZrmI AGTACT 1 cut(s) 1529
Zsp2I ATGCAT 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.