MD17G1041000.v1.1

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012381
Physical Location & Seq
Reverse (-)
2979487 .. 2982673
3187 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1041000.v1.1.491

Sequence Viewer

Length: 1632 bp
ATGGCTGGATCAGGAGGAAAAAGACTCGGCAACTCTTGCATTCTGCTGATAGTGATTGCTGGGATGGAGAGATTTGCGTACAAAGGAGTGGCATCGAATTTAGTTACTTATTTAACCGATGTGGTACAAATGAGCAACTCTGCGGCGGCCAAGACGGTGAACAGTTGGTGTGGGTTCACATCCATGCTCCCACTCTTAGTTGCACCCCTTGTTGACTCTTACTGGGACAGATATCCCACCATCTTGGCCTCTTCTTTCCTCTATGTTGCAGGGCTTGTGGCATTGACATCAACAGCATTCACCCGGGCATCATCTGCTGCAAACAAAACAAGTTCCAATTCATCATTTCTGTTTTGGTCTCTGTATTTGATTTCTGTTGGTCAAGGTGGATACAACCCATCTCTACAAGCATTTGGAGCAGACCAACTGGACAGTGAAGAGGAACTCCCTAGCAGCAAAGATGACCAAAAATCTAACAAAAAGAGTGTGTTTTTTCAGTGGTGGTATTTTGGTGTCTGCAGTGGCAGCCTCATGGGTGTCACTCTCATGTCCTACATTCAAGACACTTTTGGTTGGATTCTAGGTTTCGCCATCCCCATGATATCGATGGTTATATCCATCGTGGTATTTTCCTGTGGAAGCCGAATCTATACATATAGAGAGAATGATCAGGCCATGGATTATAAGCCTGTTTTCAACATAGCTCAAACCATCAAGGCAGCTGCATTAAAGCTAATGAAATGCAGAATCACATTACCAAATGAAACTAATGTTACTGAGCTAGAGCTTCAAGAGAAACCGCTTTGCCATCAAAATATTAGCAGCACAGCGAGCTTGGTTGAGAATCCCAAAAGCCGCTTCTACGTGATTGAAAATGCAAAAGTGCTTTTGAGGCTTCTGCCAATATGGATTATGCTTTTGATGTTTGCAGTGATTTTCCAACAGCCTCCAACATTTTTCACCAAACAAGGCATGACAATGAAGAGGAACATTGGAAGCAACTTCAAGATTCCACCAGCAACTCTGCAAAGTGCTATTACACTTTCCATAATCCTCCTGATGCCTCTGTATGACAAAATAATGATCCCGATTACGCGTCTGGTTACATGTGGCGAAAAGGGTATCAGCGTGATGCAACGGATGGGGATTGGAATGTTCGTTTCGGTCATAGCAATGGCCATTGCAGCGGTTGTGGAAACAAAAAGGCTCAAGATCAGCAGGGAAATGGAAATGCGGGGATCTCAATCCGAGACTGTGCCGTTAAGCATCTTCTGGCTGCTGCCACAGTACATTCTTTTGGGCATTTCAGACATTTTCACCGTTGTTGGAATGCAAGAGTTTTTTTACTCTGAAGTTCCGGGAAGAATGAGAACAATGGCCTTTGCATTGTACACTAGTGTTTTTGGGGTGGGAAGTTACCTGAGTACCTTTTTGATTGCAACAGTTGAAGCAGTTACAAGTTCAGAAGGAAGGCAGAGCTGGTTCTCTGATGACATGGGTGAAGCTAGGCTTGACAAATACTACTGGTTCTTGGCATCACTAAGTGCACTTAGCTTTGTGTTTTATGTAATTTTGTCTACGTTTTACACAAGTAAGAAGGATCTGGATTTGGAAAATGAAACCTGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0043207 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

544

Amino Acids

60.49

Weight (kDa)

8.59

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 79 - 494 1.6e-79 POT family
MFS_1 PF07690 167 - 474 1e-06 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012381)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01350 AT5G14940
fragaria_vesca FvH4_6g49960
malus_domestica MD09G1040700.v1.1 MD17G1041000.v1.1
prunus_persica Prupe.3G277200_v2.0.a1 Prupe.3G277200_v2.0.a1
pyrus_communis pycom111g03160 pycom17g03740
rosa_chinensis RchiOBHm_Chr2g0170141
rosa_laevigata RLG00000021928
rosa_multiflora Rmu_sc0001605.1_g000022
rosa_roxburghii Rroxscaffold_2G00081560
rosa_rugosa Rorug02G0546100.1
rosa_samantha Rh2BG629700 Rh2CG599200 Rh2DG641600
rosa_wichuraiana Rw0G018340 Rw2G051190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 684
AccI GTMKAC 1 cut(s) 1577
AccII CGCG 1 cut(s) 1096
AciI CCGC 6 cut(s) 143, 146, 800, 856, 1187, 1234
AclWI GGATC 4 cut(s) 16, 1078, 1246, 1608
AcoI YGGCCR 2 cut(s) 147, 1176
AcsI RAATTY 1 cut(s) 97
AcuI CTGAAG 1 cut(s) 1371
AdeI CACNNNGTG 1 cut(s) 1544
AfaI GTAC 5 cut(s) 80, 126, 1289, 1391, 1426
AflIII ACRYGT 2 cut(s) 1094, 1106
AgsI TTSAA 6 cut(s) 560, 697, 791, 872, 1006, 1448
AhlI ACTAGT 1 cut(s) 1394
AjuI GAANNNNNNNTTGG 2 cut(s) 842, 874
AluBI AGCT 9 cut(s) 704, 722, 733, 781, 787, 834, 1479, 1505, 1554
AluI AGCT 9 cut(s) 704, 722, 733, 781, 787, 834, 1479, 1505, 1554
Alw21I GWGCWC 1 cut(s) 1549
Alw26I GTCTC 2 cut(s) 363, 1244
Alw44I GTGCAC 1 cut(s) 1545
AlwI GGATC 4 cut(s) 16, 1078, 1246, 1608
Ama87I CYCGRG 1 cut(s) 303
AoxI GGCC 5 cut(s) 147, 246, 672, 1176, 1377
ApaLI GTGCAC 1 cut(s) 1545
ApeKI GCWGC 9 cut(s) 317, 453, 525, 719, 722, 822, 1184, 1276, 1279
ApoI RAATTY 1 cut(s) 97
AsuC2I CCSGG 3 cut(s) 304, 305, 1359
AsuHPI GGTGA 5 cut(s) 169, 292, 952, 1309, 1511
AvaI CYCGRG 1 cut(s) 303
BaeGI GKGCMC 1 cut(s) 1549
BaeI ACNNNNGTAYC 2 cut(s) 1408, 1441
BalI TGGCCA 1 cut(s) 1178
Bbv12I GWGCWC 1 cut(s) 1549
BbvI GCAGC 9 cut(s) 304, 465, 537, 709, 731, 834, 1196, 1263, 1266
BccI CCATC 9 cut(s) 58, 248, 406, 599, 601, 626, 719, 816, 1135
BceAI ACGGC 1 cut(s) 1243
BciVI GTATCC 1 cut(s) 383
BclI TGATCA 1 cut(s) 667
BcnI CCSGG 3 cut(s) 304, 305, 1359
BcoDI GTCTC 2 cut(s) 363, 1244
BcuI ACTAGT 1 cut(s) 1394
BfaI CTAG 5 cut(s) 450, 581, 782, 1395, 1506
BfmI CTRYAG 1 cut(s) 517
BfuI GTATCC 1 cut(s) 383
Bme1390I CCNGG 3 cut(s) 304, 305, 1359
BmeT110I CYCGRG 1 cut(s) 303
BmrFI CCNGG 3 cut(s) 304, 305, 1359
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 6 cut(s) 101, 317, 1050, 1122, 1275, 1544
BmuI ACTGGG 1 cut(s) 232
BpuEI CTTGAG 1 cut(s) 1193
BpuMI CCSGG 3 cut(s) 304, 305, 1359
Bsa29I ATCGAT 1 cut(s) 605
BsaAI YACGTR 1 cut(s) 865
BsaBI GATNNNNATC 1 cut(s) 1243
BsaI GGTCTC 1 cut(s) 363
BsaJI CCNNGG 2 cut(s) 303, 675
Bse1I ACTGG 3 cut(s) 227, 432, 1529
Bse3DI GCAATG 2 cut(s) 1179, 1179
Bse8I GATNNNNATC 1 cut(s) 1243
BseCI ATCGAT 1 cut(s) 605
BseDI CCNNGG 2 cut(s) 303, 675
BseGI GGATG 4 cut(s) 69, 179, 591, 1146
BseJI GATNNNNATC 1 cut(s) 1243
BseMI GCAATG 2 cut(s) 1179, 1179
BseMII CTCAG 2 cut(s) 768, 1412
BseNI ACTGG 3 cut(s) 227, 432, 1529
BseSI GKGCMC 1 cut(s) 1549
BseXI GCAGC 9 cut(s) 304, 465, 537, 709, 731, 834, 1196, 1263, 1266
BseYI CCCAGC 1 cut(s) 59
Bsh1236I CGCG 1 cut(s) 1096
BshFI GGCC 5 cut(s) 149, 248, 674, 1178, 1379
BshVI ATCGAT 1 cut(s) 605
BsiHKAI GWGCWC 1 cut(s) 1549
BsiHKCI CYCGRG 1 cut(s) 303
BsiSI CCGG 2 cut(s) 304, 1358
BslFI GGGAC 1 cut(s) 239
BsmAI GTCTC 2 cut(s) 363, 1244
BsmFI GGGAC 1 cut(s) 239
BsmI GAATGC 3 cut(s) 39, 296, 1335
BsnI GGCC 5 cut(s) 149, 248, 674, 1178, 1379
Bso31I GGTCTC 1 cut(s) 363
BsoBI CYCGRG 1 cut(s) 303
Bsp1286I GDGCHC 1 cut(s) 1549
Bsp1407I TGTACA 1 cut(s) 1389
Bsp143I GATC 6 cut(s) 8, 667, 1083, 1212, 1238, 1600
Bsp19I CCATGG 1 cut(s) 675
BspACI CCGC 6 cut(s) 143, 146, 800, 856, 1187, 1234
BspANI GGCC 5 cut(s) 149, 248, 674, 1178, 1379
BspCNI CTCAG 2 cut(s) 769, 1413
BspDI ATCGAT 1 cut(s) 605
BspFNI CGCG 1 cut(s) 1096
BspMAI CTGCAG 1 cut(s) 521
BspPI GGATC 4 cut(s) 16, 1078, 1246, 1608
BspTNI GGTCTC 1 cut(s) 363
BsrDI GCAATG 2 cut(s) 1179, 1179
BsrGI TGTACA 1 cut(s) 1389
BsrI ACTGG 3 cut(s) 227, 432, 1529
BssECI CCNNGG 2 cut(s) 303, 675
BssMI GATC 6 cut(s) 8, 667, 1083, 1212, 1238, 1600
BssT1I CCWWGG 1 cut(s) 675
Bst4CI ACNGT 7 cut(s) 157, 164, 434, 1255, 1287, 1321, 1444
Bst6I CTCTTC 3 cut(s) 256, 432, 977
BstAPI GCANNNNNTGC 2 cut(s) 36, 314
BstAUI TGTACA 1 cut(s) 1389
BstBAI YACGTR 1 cut(s) 865
BstC8I GCNNGC 1 cut(s) 832
BstDEI CTNAG 5 cut(s) 196, 777, 1421, 1541, 1550
BstDSI CCRYGG 1 cut(s) 675
BstF5I GGATG 4 cut(s) 69, 179, 591, 1146
BstFNI CGCG 1 cut(s) 1096
BstKTI GATC 6 cut(s) 11, 670, 1086, 1215, 1241, 1603
BstMAI GTCTC 2 cut(s) 363, 1244
BstMBI GATC 6 cut(s) 8, 667, 1083, 1212, 1238, 1600
BstMWI GCNNNNNNNGC 7 cut(s) 36, 314, 416, 525, 831, 892, 1184
BstNSI RCATGY 1 cut(s) 1110
BstSCI CCNGG 3 cut(s) 302, 303, 1357
BstSFI CTRYAG 1 cut(s) 517
BstSLI GKGCMC 1 cut(s) 1549
BstUI CGCG 1 cut(s) 1096
BstV1I GCAGC 9 cut(s) 304, 465, 537, 709, 731, 834, 1196, 1263, 1266
BstX2I RGATCY 2 cut(s) 1238, 1600
BstXI CCANNNNNNTGG 1 cut(s) 244
BstYI RGATCY 2 cut(s) 1238, 1600
Bsu15I ATCGAT 1 cut(s) 605
BsuI GTATCC 1 cut(s) 383
BsuRI GGCC 5 cut(s) 149, 248, 674, 1178, 1379
BsuTUI ATCGAT 1 cut(s) 605
BtgI CCRYGG 1 cut(s) 675
BtsCI GGATG 4 cut(s) 69, 179, 591, 1146
BtsI GCAGTG 2 cut(s) 526, 936
BtsIMutI CAGTG 4 cut(s) 439, 503, 526, 936
Cac8I GCNNGC 1 cut(s) 832
Cfr9I CCCGGG 1 cut(s) 303
ClaI ATCGAT 1 cut(s) 605
CseI GACGC 1 cut(s) 1085
Csp6I GTAC 5 cut(s) 79, 125, 1288, 1390, 1425
CviAII CATG 8 cut(s) 184, 532, 547, 598, 676, 973, 1107, 1495
CviQI GTAC 5 cut(s) 79, 125, 1288, 1390, 1425
DdeI CTNAG 5 cut(s) 196, 777, 1421, 1541, 1550
DpnI GATC 6 cut(s) 10, 669, 1085, 1214, 1240, 1602
DpnII GATC 6 cut(s) 8, 667, 1083, 1212, 1238, 1600
DraIII CACNNNGTG 1 cut(s) 1544
EaeI YGGCCR 2 cut(s) 147, 1176
Eam1104I CTCTTC 3 cut(s) 256, 432, 977
EarI CTCTTC 3 cut(s) 256, 432, 977
Eco130I CCWWGG 1 cut(s) 675
Eco31I GGTCTC 1 cut(s) 363
Eco32I GATATC 2 cut(s) 233, 603
Eco57I CTGAAG 1 cut(s) 1371
Eco88I CYCGRG 1 cut(s) 303
EcoRV GATATC 2 cut(s) 233, 603
EcoT14I CCWWGG 1 cut(s) 675
ErhI CCWWGG 1 cut(s) 675
FaeI CATG 8 cut(s) 187, 535, 550, 601, 679, 976, 1110, 1498
FalI AAGNNNNNCTT 2 cut(s) 1494, 1526
FaqI GGGAC 1 cut(s) 239
FatI CATG 8 cut(s) 183, 531, 546, 597, 675, 972, 1106, 1494
FauI CCCGC 1 cut(s) 1227
FbaI TGATCA 1 cut(s) 667
FblI GTMKAC 1 cut(s) 1577
FokI GGATG 4 cut(s) 76, 166, 578, 1153
FspBI CTAG 5 cut(s) 450, 581, 782, 1395, 1506
GsaI CCCAGC 1 cut(s) 63
HaeIII GGCC 5 cut(s) 149, 248, 674, 1178, 1379
HapII CCGG 2 cut(s) 304, 1358
HgaI GACGC 1 cut(s) 1085
Hin1II CATG 8 cut(s) 187, 535, 550, 601, 679, 976, 1110, 1498
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 7 cut(s) 24, 215, 577, 645, 747, 844, 1009
HpaII CCGG 2 cut(s) 304, 1358
HphI GGTGA 5 cut(s) 169, 292, 952, 1309, 1511
Hpy166II GTNNAC 6 cut(s) 160, 177, 214, 1392, 1547, 1578
Hpy188I TCNGA 5 cut(s) 1249, 1309, 1351, 1465, 1489
Hpy188III TCNNGA 8 cut(s) 12, 560, 791, 1006, 1057, 1087, 1210, 1604
Hpy8I GTNNAC 6 cut(s) 160, 177, 214, 1392, 1547, 1578
HpyAV CCTTC 3 cut(s) 1460, 1464, 1591
HpyCH4III ACNGT 7 cut(s) 157, 164, 434, 1255, 1287, 1321, 1444
HpyCH4IV ACGT 2 cut(s) 864, 1580
HpyF10VI GCNNNNNNNGC 7 cut(s) 36, 314, 416, 525, 831, 892, 1184
HpyF3I CTNAG 5 cut(s) 196, 777, 1421, 1541, 1550
HpySE526I ACGT 2 cut(s) 864, 1580
Hsp92II CATG 8 cut(s) 187, 535, 550, 601, 679, 976, 1110, 1498
Ksp22I TGATCA 1 cut(s) 667
Kzo9I GATC 6 cut(s) 8, 667, 1083, 1212, 1238, 1600
LmnI GCTCC 2 cut(s) 192, 416
Lsp1109I GCAGC 9 cut(s) 304, 465, 537, 709, 731, 834, 1196, 1263, 1266
LweI GCATC 6 cut(s) 101, 317, 1050, 1122, 1275, 1544
MaeI CTAG 5 cut(s) 450, 581, 782, 1395, 1506
MaeII ACGT 2 cut(s) 864, 1580
MaeIII GTNAC 6 cut(s) 103, 538, 772, 1102, 1415, 1453
MalI GATC 6 cut(s) 10, 669, 1085, 1214, 1240, 1602
MboI GATC 6 cut(s) 8, 667, 1083, 1212, 1238, 1600
MboII GAAGA 5 cut(s) 243, 449, 994, 1261, 1374
MflI RGATCY 2 cut(s) 1238, 1600
MhlI GDGCHC 1 cut(s) 1549
MlsI TGGCCA 1 cut(s) 1178
MluCI AATT 3 cut(s) 97, 337, 1569
MluI ACGCGT 1 cut(s) 1094
MluNI TGGCCA 1 cut(s) 1178
MlyI GAGTC 2 cut(s) 18, 209
MmeI TCCRAC 4 cut(s) 554, 964, 974, 1306
Mox20I TGGCCA 1 cut(s) 1178
MscI TGGCCA 1 cut(s) 1178
MseI TTAA 3 cut(s) 113, 728, 1262
MslI CAYNNNNRTG 5 cut(s) 182, 545, 596, 977, 1172
Msp20I TGGCCA 1 cut(s) 1178
MspA1I CMGCKG 2 cut(s) 722, 1187
MspI CCGG 2 cut(s) 304, 1358
MspR9I CCNGG 3 cut(s) 304, 305, 1359
Mva1269I GAATGC 3 cut(s) 39, 296, 1335
MvnI CGCG 1 cut(s) 1096
MwoI GCNNNNNNNGC 7 cut(s) 36, 314, 416, 525, 831, 892, 1184
NciI CCSGG 3 cut(s) 304, 305, 1359
NcoI CCATGG 1 cut(s) 675
NdeII GATC 6 cut(s) 8, 667, 1083, 1212, 1238, 1600
NlaIII CATG 8 cut(s) 187, 535, 550, 601, 679, 976, 1110, 1498
NmeAIII GCCGAG 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 538
NspI RCATGY 1 cut(s) 1110
PciI ACATGT 1 cut(s) 1106
PctI GAATGC 3 cut(s) 39, 296, 1335
PfeI GAWTC 5 cut(s) 577, 645, 747, 844, 1009
PfoI TCCNGGA 1 cut(s) 1357
PleI GAGTC 2 cut(s) 18, 209
PpsI GAGTC 2 cut(s) 18, 209
Ppu21I YACGTR 1 cut(s) 865
PscI ACATGT 1 cut(s) 1106
PsiI TTATAA 1 cut(s) 684
PspFI CCCAGC 1 cut(s) 59
PstI CTGCAG 1 cut(s) 521
PsuI RGATCY 2 cut(s) 1238, 1600
PvuII CAGCTG 1 cut(s) 722
RsaI GTAC 5 cut(s) 80, 126, 1289, 1391, 1426
RsaNI GTAC 5 cut(s) 79, 125, 1288, 1390, 1425
RseI CAYNNNNRTG 5 cut(s) 182, 545, 596, 977, 1172
SaqAI TTAA 3 cut(s) 113, 728, 1262
Sau3AI GATC 6 cut(s) 8, 667, 1083, 1212, 1238, 1600
SchI GAGTC 2 cut(s) 18, 209
ScrFI CCNGG 3 cut(s) 304, 305, 1359
SduI GDGCHC 1 cut(s) 1549
SfaNI GCATC 6 cut(s) 101, 317, 1050, 1122, 1275, 1544
SfcI CTRYAG 1 cut(s) 517
SmaI CCCGGG 1 cut(s) 305
SmiMI CAYNNNNRTG 5 cut(s) 182, 545, 596, 977, 1172
SmlI CTYRAG 1 cut(s) 1208
SmoI CTYRAG 1 cut(s) 1208
SpeI ACTAGT 1 cut(s) 1394
Sse9I AATT 3 cut(s) 97, 337, 1569
SsiI CCGC 6 cut(s) 143, 146, 800, 856, 1187, 1234
SspI AATATT 1 cut(s) 817
SspMI CTAG 5 cut(s) 450, 581, 782, 1395, 1506
StyD4I CCNGG 3 cut(s) 302, 303, 1357
StyI CCWWGG 1 cut(s) 675
TaaI ACNGT 7 cut(s) 157, 164, 434, 1255, 1287, 1321, 1444
TaiI ACGT 2 cut(s) 867, 1583
TaqI TCGA 2 cut(s) 95, 605
TaqII GACCGA 1 cut(s) 1153
TasI AATT 3 cut(s) 97, 337, 1569
TatI WGTACW 2 cut(s) 1287, 1389
TauI GCSGC 3 cut(s) 146, 149, 858
TfiI GAWTC 5 cut(s) 577, 645, 747, 844, 1009
Tru1I TTAA 3 cut(s) 113, 728, 1262
Tru9I TTAA 3 cut(s) 113, 728, 1262
TscAI CASTG 4 cut(s) 439, 503, 526, 936
TseFI GTSAC 1 cut(s) 538
TseI GCWGC 9 cut(s) 317, 453, 525, 719, 722, 822, 1184, 1276, 1279
Tsp45I GTSAC 1 cut(s) 538
TspDTI ATGAA 5 cut(s) 330, 752, 777, 995, 1632
TspGWI ACGGA 1 cut(s) 1153
TspMI CCCGGG 1 cut(s) 303
TspRI CASTG 4 cut(s) 439, 503, 526, 936
VneI GTGCAC 1 cut(s) 1545
XapI RAATTY 1 cut(s) 97
XceI RCATGY 1 cut(s) 1110
XcmI CCANNNNNNNNNTGG 1 cut(s) 604
XmaI CCCGGG 1 cut(s) 303
XmiI GTMKAC 1 cut(s) 1577
XspI CTAG 5 cut(s) 450, 581, 782, 1395, 1506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.