Rw0G018340

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00818
OG0012381
Physical Location & Seq
Forward (+)
8400 .. 11476
3077 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G018340.1

Sequence Viewer

Length: 1494 bp
ATGGCTGGTTCAACAAGAAAGAGACTCGGCAACTCATGCATTCTTCTCATAGTAATTGCTGGTATGGAAAGATTTGCATACAAAGGAGTGGCATCCAATCTGATAACTTATCTGACGGATGTAGTAAAGATGAGCAACTCTTCTGCGGCGAAGACGGTGAACAGGTGGTGCGGATTCACATCCATGGTTCCACTCTTGATTGCACCCCTTGCTGATTCTTACTGGGATAGATATTACACCATTGTAGCCTCTTGTTTTATCTATGTTGCCTTCATCTCATTTCTGTCTGCTCATTGTATTTTGATTTCTCTTGGTCAAGGTGGCTACAACCCATCTTTGCAAGCTTTTGGAGCAGATCAACTTGATAGTGAAGATGAAGAGTTGCCTAGTAGCAAAGATGACAAAAAATCCAACAAAAAGAGTTTTGGCACCGTCTTGGGTATAAGTGTTATGTTCCTACATTCAAGATACTTTTGGTTGGATACTAGGTTTTGCCATCCCCATGATTTCGATGGTCCATCAGTCATAGTTTTCTCGTGTGGAAGCCCAATTTATACATATAGACAGAGAAATGAGACCATGGGTAATAAGCCAGTTGTGAGCATGCTTCAAGTTTTTAAGGCAGCTGCATTGAAATTACTGAATTCCAAAGTCAAGTTACCCGATGAAAATGATGTTACTGAGCTAGAGCTTCAAGGGAAACCGCTCTGTCGACATCAGAACTTAAGCAGCAATGAAGGCTTGAACAATGAGCATCCCAAAAGCAGCATCCACATGTTCGAAAATGCAAAAGTTATTCTTAGGCTTTTGCCCATATGGCTAATGCTTTTAATGTTTGCAGTGATTTTCCAGCAACCTTCGACATTCTTCACCAAACAAGGCATGACAATGAAGAGGAATATCGGAAGCAATTTTCTGATTCCACCCGCAACACTGCAGAGTGCTATTGCACTTTCTATAGTTGTGTTGATGCCTCTTTATGACAAAGTTCTGATCCCAATTACTAAGGTCCTGACTTGTAACGAAAAGGCACTGATCATTGCAGCACTTGTAGAGACAAGAAGGCTCCATATCAGCAGAAATATGGAAATCCTAGGATCTCAATCTGAGACGGTTCCATTAAGCATATTCTGGTTGCTGCCACAGTACATTCTTCTGGGCATTTCGGACATTTTCACTGTCGTTGGGATGCAAGAGTTCTTTTACTCTGAATTTCCCATTAGAATGAGAACAATGGCCTTTGCACTATACACCAGTGTTTTTGGGGTCGGGAGCTACTTGAGTGCCCTTTTGATCTCAACAGTTGAAGGCATCACAAGTTCAAATGGAAGTAAGAGCTGGTTCTGTGATGATATGAATGAAGCAAGGCTAGACAAGTACTACTGGTTTTTAGCAGCAGCAAGTGCACTGAGCTTTCTGTTTTTTGTGATTATTTCATCATTTTACACAAGTAAGAGGGATTTGGAGTTGGACGGTAATGAAAGTTGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0043207 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

497

Amino Acids

55.74

Weight (kDa)

8.4

Isoelectric Point (pI)

41.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 174 - 447 2e-31 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012381)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01350 AT5G14940
fragaria_vesca FvH4_6g49960
malus_domestica MD09G1040700.v1.1 MD17G1041000.v1.1
prunus_persica Prupe.3G277200_v2.0.a1 Prupe.3G277200_v2.0.a1
pyrus_communis pycom111g03160 pycom17g03740
rosa_chinensis RchiOBHm_Chr2g0170141
rosa_laevigata RLG00000021928
rosa_multiflora Rmu_sc0001605.1_g000022
rosa_roxburghii Rroxscaffold_2G00081560
rosa_rugosa Rorug02G0546100.1
rosa_samantha Rh2BG629700 Rh2CG599200 Rh2DG641600
rosa_wichuraiana Rw0G018340 Rw2G051190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 428
AccBSI CCGCTC 1 cut(s) 706
AccI GTMKAC 1 cut(s) 712
AciI CCGC 4 cut(s) 146, 171, 704, 927
AclWI GGATC 2 cut(s) 988, 1105
AcsI RAATTY 2 cut(s) 643, 1211
AfaI GTAC 2 cut(s) 1148, 1379
AflII CTTAAG 1 cut(s) 724
AflIII ACRYGT 1 cut(s) 774
AgsI TTSAA 8 cut(s) 12, 465, 611, 634, 695, 745, 1307, 1323
AluBI AGCT 7 cut(s) 344, 626, 685, 691, 1275, 1338, 1413
AluI AGCT 7 cut(s) 344, 626, 685, 691, 1275, 1338, 1413
Alw21I GWGCWC 1 cut(s) 1408
Alw26I GTCTC 4 cut(s) 16, 569, 1049, 1103
Alw44I GTGCAC 1 cut(s) 1404
AlwI GGATC 2 cut(s) 988, 1105
AoxI GGCC 1 cut(s) 1236
ApaLI GTGCAC 1 cut(s) 1404
ApeKI GCWGC 8 cut(s) 623, 626, 729, 765, 1043, 1138, 1394, 1397
ApoI RAATTY 2 cut(s) 643, 1211
AspA2I CCTAGG 1 cut(s) 1093
AspS9I GGNCC 2 cut(s) 515, 1009
AsuHPI GGTGA 2 cut(s) 169, 862
AsuII TTCGAA 1 cut(s) 780
AvaII GGWCC 2 cut(s) 515, 1009
AvrII CCTAGG 1 cut(s) 1093
BaeGI GKGCMC 2 cut(s) 1288, 1408
BanI GGYRCC 1 cut(s) 428
BauI CACGAG 1 cut(s) 535
BbsI GAAGAC 1 cut(s) 158
Bbv12I GWGCWC 1 cut(s) 1408
BbvI GCAGC 8 cut(s) 613, 635, 741, 777, 1055, 1125, 1406, 1409
BccI CCATC 4 cut(s) 340, 504, 506, 526
BciVI GTATCC 1 cut(s) 475
BclI TGATCA 1 cut(s) 1035
BcoDI GTCTC 4 cut(s) 16, 569, 1049, 1103
BfaI CTAG 5 cut(s) 387, 486, 686, 1094, 1370
BfmI CTRYAG 2 cut(s) 935, 957
BfrI CTTAAG 1 cut(s) 724
BfuI GTATCC 1 cut(s) 475
BglI GCCNNNNNGGC 1 cut(s) 817
BisI GCNGC 9 cut(s) 147, 624, 627, 730, 766, 1044, 1139, 1395, 1398
BlnI CCTAGG 1 cut(s) 1093
BlsI GCNGC 9 cut(s) 148, 625, 628, 731, 767, 1045, 1140, 1396, 1399
BmcAI AGTACT 1 cut(s) 1379
Bme18I GGWCC 2 cut(s) 515, 1009
BmgT120I GGNCC 2 cut(s) 515, 1009
BmiI GGNNCC 4 cut(s) 189, 430, 1067, 1116
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 6 cut(s) 101, 763, 777, 960, 1179, 1320
BmuI ACTGGG 1 cut(s) 232
BpiI GAAGAC 1 cut(s) 158
Bpu14I TTCGAA 1 cut(s) 780
BpuEI CTTGAG 1 cut(s) 1300
BsaBI GATNNNNATC 2 cut(s) 178, 1102
BsaI GGTCTC 1 cut(s) 569
BsaJI CCNNGG 3 cut(s) 183, 579, 1093
Bse1I ACTGG 4 cut(s) 227, 593, 1254, 1388
Bse3DI GCAATG 2 cut(s) 739, 1038
Bse8I GATNNNNATC 2 cut(s) 178, 1102
BseDI CCNNGG 3 cut(s) 183, 579, 1093
BseGI GGATG 7 cut(s) 92, 124, 179, 496, 754, 768, 1194
BseJI GATNNNNATC 2 cut(s) 178, 1102
BseMI GCAATG 2 cut(s) 739, 1038
BseMII CTCAG 3 cut(s) 672, 1098, 1400
BseNI ACTGG 4 cut(s) 227, 593, 1254, 1388
BseSI GKGCMC 2 cut(s) 1288, 1408
BseXI GCAGC 8 cut(s) 613, 635, 741, 777, 1055, 1125, 1406, 1409
BshFI GGCC 1 cut(s) 1238
BshNI GGYRCC 1 cut(s) 428
BsiHKAI GWGCWC 1 cut(s) 1408
BsmAI GTCTC 4 cut(s) 16, 569, 1049, 1103
BsmBI CGTCTC 1 cut(s) 1103
BsmI GAATGC 1 cut(s) 39
BsnI GGCC 1 cut(s) 1238
Bso31I GGTCTC 1 cut(s) 569
Bsp119I TTCGAA 1 cut(s) 780
Bsp1286I GDGCHC 2 cut(s) 1288, 1408
Bsp143I GATC 5 cut(s) 355, 993, 1035, 1097, 1293
Bsp19I CCATGG 2 cut(s) 183, 579
BspACI CCGC 4 cut(s) 146, 171, 704, 927
BspANI GGCC 1 cut(s) 1238
BspCNI CTCAG 3 cut(s) 673, 1099, 1401
BspLI GGNNCC 4 cut(s) 189, 430, 1067, 1116
BspMAI CTGCAG 1 cut(s) 939
BspPI GGATC 2 cut(s) 988, 1105
BspT104I TTCGAA 1 cut(s) 780
BspT107I GGYRCC 1 cut(s) 428
BspTI CTTAAG 1 cut(s) 724
BspTNI GGTCTC 1 cut(s) 569
BsrBI CCGCTC 1 cut(s) 706
BsrDI GCAATG 2 cut(s) 739, 1038
BsrI ACTGG 4 cut(s) 227, 593, 1254, 1388
BssECI CCNNGG 3 cut(s) 183, 579, 1093
BssMI GATC 5 cut(s) 355, 993, 1035, 1097, 1293
BssSI CACGAG 1 cut(s) 535
BssT1I CCWWGG 3 cut(s) 183, 579, 1093
Bst2BI CACGAG 1 cut(s) 535
Bst4CI ACNGT 7 cut(s) 157, 433, 1114, 1146, 1180, 1303, 1475
Bst6I CTCTTC 3 cut(s) 145, 372, 887
BstAFI CTTAAG 1 cut(s) 724
BstAPI GCANNNNNTGC 3 cut(s) 36, 209, 1403
BstBI TTCGAA 1 cut(s) 780
BstC8I GCNNGC 2 cut(s) 342, 605
BstDEI CTNAG 5 cut(s) 681, 800, 1005, 1107, 1409
BstDSI CCRYGG 2 cut(s) 183, 579
BstF5I GGATG 7 cut(s) 92, 124, 179, 496, 754, 768, 1194
BstKTI GATC 5 cut(s) 358, 996, 1038, 1100, 1296
BstMAI GTCTC 4 cut(s) 16, 569, 1049, 1103
BstMBI GATC 5 cut(s) 355, 993, 1035, 1097, 1293
BstMWI GCNNNNNNNGC 6 cut(s) 36, 209, 350, 738, 817, 1403
BstNSI RCATGY 2 cut(s) 607, 778
BstSFI CTRYAG 2 cut(s) 935, 957
BstSLI GKGCMC 2 cut(s) 1288, 1408
BstV1I GCAGC 8 cut(s) 613, 635, 741, 777, 1055, 1125, 1406, 1409
BstV2I GAAGAC 1 cut(s) 158
BstX2I RGATCY 1 cut(s) 1097
BstYI RGATCY 1 cut(s) 1097
BsuI GTATCC 1 cut(s) 475
BsuRI GGCC 1 cut(s) 1238
BtgI CCRYGG 2 cut(s) 183, 579
BtsCI GGATG 7 cut(s) 92, 124, 179, 496, 754, 768, 1194
BtsI GCAGTG 2 cut(s) 846, 932
BtsIMutI CAGTG 6 cut(s) 846, 932, 1031, 1176, 1261, 1406
Cac8I GCNNGC 2 cut(s) 342, 605
Cfr13I GGNCC 2 cut(s) 515, 1009
Csp6I GTAC 2 cut(s) 1147, 1378
CviAII CATG 7 cut(s) 36, 184, 503, 580, 604, 775, 883
CviQI GTAC 2 cut(s) 1147, 1378
DdeI CTNAG 5 cut(s) 681, 800, 1005, 1107, 1409
DpnI GATC 5 cut(s) 357, 995, 1037, 1099, 1295
DpnII GATC 5 cut(s) 355, 993, 1035, 1097, 1293
Eam1104I CTCTTC 3 cut(s) 145, 372, 887
EarI CTCTTC 3 cut(s) 145, 372, 887
Eco130I CCWWGG 3 cut(s) 183, 579, 1093
Eco31I GGTCTC 1 cut(s) 569
Eco47I GGWCC 2 cut(s) 515, 1009
EcoO109I RGGNCCY 1 cut(s) 1009
EcoRI GAATTC 1 cut(s) 643
EcoT14I CCWWGG 3 cut(s) 183, 579, 1093
EcoT22I ATGCAT 1 cut(s) 41
ErhI CCWWGG 3 cut(s) 183, 579, 1093
Esp3I CGTCTC 1 cut(s) 1103
FaeI CATG 7 cut(s) 39, 187, 506, 583, 607, 778, 886
FalI AAGNNNNNCTT 2 cut(s) 783, 815
FatI CATG 7 cut(s) 35, 183, 502, 579, 603, 774, 882
FauI CCCGC 1 cut(s) 934
FauNDI CATATG 1 cut(s) 815
FbaI TGATCA 1 cut(s) 1035
FblI GTMKAC 1 cut(s) 712
Fnu4HI GCNGC 9 cut(s) 147, 624, 627, 730, 766, 1044, 1139, 1395, 1398
FokI GGATG 7 cut(s) 79, 131, 166, 483, 741, 755, 1201
Fsp4HI GCNGC 9 cut(s) 147, 624, 627, 730, 766, 1044, 1139, 1395, 1398
FspBI CTAG 5 cut(s) 387, 486, 686, 1094, 1370
GluI GCNGC 9 cut(s) 147, 624, 627, 730, 766, 1044, 1139, 1395, 1398
HaeIII GGCC 1 cut(s) 1238
Hin1II CATG 7 cut(s) 39, 187, 506, 583, 607, 778, 886
HincII GTYRAC 1 cut(s) 713
HindII GTYRAC 1 cut(s) 713
HindIII AAGCTT 1 cut(s) 342
HinfI GANTC 4 cut(s) 24, 174, 215, 919
HphI GGTGA 2 cut(s) 169, 862
Hpy166II GTNNAC 3 cut(s) 160, 713, 1406
Hpy188I TCNGA 9 cut(s) 102, 114, 720, 905, 918, 993, 1108, 1168, 1210
Hpy188III TCNNGA 4 cut(s) 196, 465, 1012, 1270
Hpy8I GTNNAC 3 cut(s) 160, 713, 1406
HpyAV CCTTC 5 cut(s) 280, 731, 867, 1056, 1301
HpyCH4III ACNGT 7 cut(s) 157, 433, 1114, 1146, 1180, 1303, 1475
HpyF10VI GCNNNNNNNGC 6 cut(s) 36, 209, 350, 738, 817, 1403
HpyF3I CTNAG 5 cut(s) 681, 800, 1005, 1107, 1409
Hsp92II CATG 7 cut(s) 39, 187, 506, 583, 607, 778, 886
Ksp22I TGATCA 1 cut(s) 1035
Kzo9I GATC 5 cut(s) 355, 993, 1035, 1097, 1293
LmnI GCTCC 3 cut(s) 350, 1071, 1272
Lsp1109I GCAGC 8 cut(s) 613, 635, 741, 777, 1055, 1125, 1406, 1409
LweI GCATC 6 cut(s) 101, 763, 777, 960, 1179, 1320
MaeI CTAG 5 cut(s) 387, 486, 686, 1094, 1370
MaeIII GTNAC 3 cut(s) 657, 676, 1019
MalI GATC 5 cut(s) 357, 995, 1037, 1099, 1295
MbiI CCGCTC 1 cut(s) 706
MboI GATC 5 cut(s) 355, 993, 1035, 1097, 1293
MboII GAAGA 8 cut(s) 35, 132, 163, 383, 389, 859, 904, 1145
MflI RGATCY 1 cut(s) 1097
MhlI GDGCHC 2 cut(s) 1288, 1408
MluCI AATT 7 cut(s) 54, 549, 635, 643, 910, 999, 1211
MlyI GAGTC 1 cut(s) 18
MmeI TCCRAC 3 cut(s) 435, 459, 1449
MnlI CCTC 4 cut(s) 259, 888, 984, 1449
Mph1103I ATGCAT 1 cut(s) 41
MseI TTAA 4 cut(s) 618, 725, 830, 1121
MslI CAYNNNNRTG 5 cut(s) 182, 501, 773, 887, 1223
MspA1I CMGCKG 1 cut(s) 626
MspCI CTTAAG 1 cut(s) 724
Mva1269I GAATGC 1 cut(s) 39
MwoI GCNNNNNNNGC 6 cut(s) 36, 209, 350, 738, 817, 1403
NcoI CCATGG 2 cut(s) 183, 579
NdeI CATATG 1 cut(s) 815
NdeII GATC 5 cut(s) 355, 993, 1035, 1097, 1293
NlaIII CATG 7 cut(s) 39, 187, 506, 583, 607, 778, 886
NlaIV GGNNCC 4 cut(s) 189, 430, 1067, 1116
NmeAIII GCCGAG 1 cut(s) 6
NsiI ATGCAT 1 cut(s) 41
NspI RCATGY 2 cut(s) 607, 778
NspV TTCGAA 1 cut(s) 780
PaeI GCATGC 1 cut(s) 607
PciI ACATGT 1 cut(s) 774
PctI GAATGC 1 cut(s) 39
PfeI GAWTC 3 cut(s) 174, 215, 919
PkrI GCNGC 9 cut(s) 148, 625, 628, 731, 767, 1045, 1140, 1396, 1399
PleI GAGTC 1 cut(s) 18
PpsI GAGTC 1 cut(s) 18
PpuMI RGGWCCY 1 cut(s) 1009
PscI ACATGT 1 cut(s) 774
Psp5II RGGWCCY 1 cut(s) 1009
PspN4I GGNNCC 4 cut(s) 189, 430, 1067, 1116
PspPI GGNCC 2 cut(s) 515, 1009
PspPPI RGGWCCY 1 cut(s) 1009
PstI CTGCAG 1 cut(s) 939
PsuI RGATCY 1 cut(s) 1097
PvuII CAGCTG 1 cut(s) 626
RsaI GTAC 2 cut(s) 1148, 1379
RsaNI GTAC 2 cut(s) 1147, 1378
RseI CAYNNNNRTG 5 cut(s) 182, 501, 773, 887, 1223
SalI GTCGAC 1 cut(s) 711
SaqAI TTAA 4 cut(s) 618, 725, 830, 1121
SatI GCNGC 9 cut(s) 147, 624, 627, 730, 766, 1044, 1139, 1395, 1398
Sau3AI GATC 5 cut(s) 355, 993, 1035, 1097, 1293
Sau96I GGNCC 2 cut(s) 515, 1009
ScaI AGTACT 1 cut(s) 1379
SchI GAGTC 1 cut(s) 18
SduI GDGCHC 2 cut(s) 1288, 1408
SfaNI GCATC 6 cut(s) 101, 763, 777, 960, 1179, 1320
SfcI CTRYAG 2 cut(s) 935, 957
SfuI TTCGAA 1 cut(s) 780
SinI GGWCC 2 cut(s) 515, 1009
SmiMI CAYNNNNRTG 5 cut(s) 182, 501, 773, 887, 1223
SmlI CTYRAG 2 cut(s) 724, 1279
SmoI CTYRAG 2 cut(s) 724, 1279
SphI GCATGC 1 cut(s) 607
Sse9I AATT 7 cut(s) 54, 549, 635, 643, 910, 999, 1211
SsiI CCGC 4 cut(s) 146, 171, 704, 927
SspMI CTAG 5 cut(s) 387, 486, 686, 1094, 1370
StyI CCWWGG 3 cut(s) 183, 579, 1093
TaaI ACNGT 7 cut(s) 157, 433, 1114, 1146, 1180, 1303, 1475
TaqI TCGA 4 cut(s) 510, 712, 780, 860
TasI AATT 7 cut(s) 54, 549, 635, 643, 910, 999, 1211
TatI WGTACW 2 cut(s) 1146, 1377
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 3 cut(s) 174, 215, 919
Tru1I TTAA 4 cut(s) 618, 725, 830, 1121
Tru9I TTAA 4 cut(s) 618, 725, 830, 1121
TscAI CASTG 6 cut(s) 846, 939, 1038, 1183, 1261, 1413
TseI GCWGC 8 cut(s) 623, 626, 729, 765, 1043, 1138, 1394, 1397
TspDTI ATGAA 9 cut(s) 262, 390, 681, 750, 905, 1370, 1374, 1425, 1494
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 6 cut(s) 846, 939, 1038, 1183, 1261, 1413
Vha464I CTTAAG 1 cut(s) 724
VneI GTGCAC 1 cut(s) 1404
VpaK11BI GGWCC 2 cut(s) 515, 1009
XapI RAATTY 2 cut(s) 643, 1211
XceI RCATGY 2 cut(s) 607, 778
XcmI CCANNNNNNNNNTGG 1 cut(s) 509
XmaJI CCTAGG 1 cut(s) 1093
XmiI GTMKAC 1 cut(s) 712
XspI CTAG 5 cut(s) 387, 486, 686, 1094, 1370
ZrmI AGTACT 1 cut(s) 1379
Zsp2I ATGCAT 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.