pycom111g03160

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0012381
Physical Location & Seq
Reverse (-)
1908003 .. 1910852
2850 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g03160.4

Sequence Viewer

Length: 1632 bp
ATGGCTGGATCAGGAGGAAAAAGACTCGGCACCTCTTGCTTTCTTCTGATAGTGATTTCTGGGATGGAGAGATTTGCATACAAAGGAGTGGCATCGAATCTAGTGACTTATTTAACCGATGTGGTGAAAATGAGCAACTCGGCAGCAGCCAAGACGGTAAACAGTTGGTGTGGGTTCACATCCATGCTCCCACTCTTAGTTGCACCCCTTGTCGACTCTTACTGGGATAGATATCCCACCATCTTGGTCTCTTCTTTCCTCTATGTTGCAGGGCTTGTGGCATTGACATCAACGGCATTCACCCGGGCAACACGTGCTGCAAACAAAGCAAGTTCCAATTCATCATTTCTGTTTTGCTCTCTGTACTTGATTTCTCTAGGTCAAGGCGGATACAACCCATCGCTGCAAGCATTTGGAGCAGACCAACTGGACGGTGAAGAGGAACTTCCTAGCAGCAAAGACGACCAAAAATCAAGCAAAAAGACAATGTTTTTTCAGTGGTGGTATTTTGGTGTTTGCAGTGGCAGCCTCATGGGTGTCACTCTCATGTCCTACATTCAAGACACTTTCGGTTGGATTATAGGTTTCGCCATCCCCATGATATCGATGGTTATATCGGTCGTGGTGTTTATGTGTGGAAGCCGAATCTATACATACAGAGAAAATGATCAGGCAATGGATTATAAGCCTATTTTCAACATAGTTCAAGTCATCAAGGCAACTGCATTAAGGTTAATGAAATGCAGAATCATATTACCGAATGAAACTAATGTTACCGAGCTAGAGCTTCAAGAGAAACCGCTTTGCTGTCAAAATAATAGCAGCACAGCAAGCTTGGTTGGGAATCCCAAAAGTGGCTTCTACGTGCTTGAAAATGCAAAAGTGCTTTTGAGGCTTCTGCCAATATGGATTATGCTTTTGATGTTTGCAGTGATTTTCCAACAGCCTCCAACATTTTTCACCAAACAAGGCATGACGATGAAGAGGAACATTGGAAGCAACTTCAAGGTTCCACCAGCAACTCTTCAAAGCGCTATCACACTTTCCATAATCCTCCTGATGCCTCTGTATGACAAAATAATGATCCCGATTACGCGTCTGGTTACGTCTAATGAAAAGGGTATTAGCGTGATGCAACGGATGGGGATCGGAATGTTCGTTTCGGTCATAGCAATGGTCATCGCAGCGGTTGTGGAAACAAAAAGGCTCAAGATCAGCAGAGAAATGGAAATGCTGGGATCTCAACCCGAAACTGTACCGTTTAGCATCTTTTGGCTGCTTCCACAGTACATTCTTCTAGGCATTTCAGACATTTTCACCGTTGTTGGAATACAAGAGTTCTTTTACTCTGAAGTCCCCAGAAGGATGAGAACAATGGCCTTTGCATTGTACACTAGCGTTTTTGGGGTGGGAAGTTACCTGAGTACCCTTTTGATTGCAACGGTTGAGGCAGTTACAAGTTCAGGAGGAAGGCAGAGCTGGTTCTCTGATGACATGAATGAAGCTAGACTTGATAAATACTACTGGTTTTTGGCCTCACTAAGTGCATTGAGCTTTGTGTTTTATGTAATTTTGTCTAGGTGTTACACAAGCATGAAGGATTTGGACTTGGAAAATGAAGTCTGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0043207 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

544

Amino Acids

60.56

Weight (kDa)

8.83

Isoelectric Point (pI)

35.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012381)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01350 AT5G14940
fragaria_vesca FvH4_6g49960
malus_domestica MD09G1040700.v1.1 MD17G1041000.v1.1
prunus_persica Prupe.3G277200_v2.0.a1 Prupe.3G277200_v2.0.a1
pyrus_communis pycom111g03160 pycom17g03740
rosa_chinensis RchiOBHm_Chr2g0170141
rosa_laevigata RLG00000021928
rosa_multiflora Rmu_sc0001605.1_g000022
rosa_roxburghii Rroxscaffold_2G00081560
rosa_rugosa Rorug02G0546100.1
rosa_samantha Rh2BG629700 Rh2CG599200 Rh2DG641600
rosa_wichuraiana Rw0G018340 Rw2G051190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 684
AccB1I GGYRCC 1 cut(s) 29
AccI GTMKAC 1 cut(s) 213
AccII CGCG 1 cut(s) 1096
AciI CCGC 3 cut(s) 387, 800, 1187
AclWI GGATC 4 cut(s) 16, 1078, 1154, 1246
AcuI CTGAAG 1 cut(s) 1371
AcvI CACGTG 1 cut(s) 314
AdeI CACNNNGTG 1 cut(s) 1544
AfaI GTAC 5 cut(s) 365, 1257, 1289, 1391, 1426
AfeI AGCGCT 1 cut(s) 1033
AfiI CCNNNNNNNGG 1 cut(s) 854
AflIII ACRYGT 2 cut(s) 311, 1094
AgsI TTSAA 7 cut(s) 560, 697, 707, 791, 872, 1006, 1028
AjuI GAANNNNNNNTTGG 2 cut(s) 842, 874
AluBI AGCT 6 cut(s) 781, 787, 834, 1479, 1505, 1554
AluI AGCT 6 cut(s) 781, 787, 834, 1479, 1505, 1554
Alw26I GTCTC 1 cut(s) 253
AlwI GGATC 4 cut(s) 16, 1078, 1154, 1246
Ama87I CYCGRG 1 cut(s) 303
Aor51HI AGCGCT 1 cut(s) 1033
AoxI GGCC 2 cut(s) 1377, 1533
ApeKI GCWGC 9 cut(s) 143, 146, 317, 403, 453, 525, 822, 1184, 1276
AspLEI GCGC 1 cut(s) 1034
AsuC2I CCSGG 2 cut(s) 304, 305
AsuHPI GGTGA 5 cut(s) 136, 292, 446, 952, 1309
AvaI CYCGRG 1 cut(s) 303
BaeI ACNNNNGTAYC 2 cut(s) 1408, 1441
BanI GGYRCC 1 cut(s) 29
BbrPI CACGTG 1 cut(s) 314
BbvI GCAGC 9 cut(s) 155, 158, 304, 390, 465, 537, 834, 1196, 1263
BccI CCATC 6 cut(s) 58, 248, 406, 599, 601, 1135
BceAI ACGGC 1 cut(s) 309
BciVI GTATCC 1 cut(s) 383
BclI TGATCA 1 cut(s) 667
BcnI CCSGG 2 cut(s) 304, 305
BcoDI GTCTC 1 cut(s) 253
BfaI CTAG 8 cut(s) 101, 377, 450, 782, 1298, 1395, 1506, 1578
BfoI RGCGCY 1 cut(s) 1035
BfuI GTATCC 1 cut(s) 383
BisI GCNGC 9 cut(s) 144, 147, 318, 404, 454, 526, 823, 1185, 1277
BlsI GCNGC 9 cut(s) 145, 148, 319, 405, 455, 527, 824, 1186, 1278
Bme1390I CCNGG 2 cut(s) 304, 305
BmeT110I CYCGRG 1 cut(s) 303
BmiI GGNNCC 2 cut(s) 31, 1011
BmrFI CCNGG 2 cut(s) 304, 305
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 4 cut(s) 101, 1050, 1122, 1275
BmuI ACTGGG 1 cut(s) 232
BpuEI CTTGAG 1 cut(s) 1193
BpuMI CCSGG 2 cut(s) 304, 305
Bsa29I ATCGAT 1 cut(s) 605
BsaAI YACGTR 2 cut(s) 314, 865
BsaBI GATNNNNATC 2 cut(s) 231, 1145
BsaI GGTCTC 1 cut(s) 253
BsaJI CCNNGG 1 cut(s) 303
Bsc4I CCNNNNNNNGG 1 cut(s) 854
Bse1I ACTGG 3 cut(s) 227, 432, 1529
Bse3DI GCAATG 2 cut(s) 681, 1179
Bse8I GATNNNNATC 2 cut(s) 231, 1145
BseCI ATCGAT 1 cut(s) 605
BseDI CCNNGG 1 cut(s) 303
BseGI GGATG 5 cut(s) 69, 179, 591, 1146, 1371
BseJI GATNNNNATC 2 cut(s) 231, 1145
BseLI CCNNNNNNNGG 1 cut(s) 854
BseMI GCAATG 2 cut(s) 681, 1179
BseMII CTCAG 1 cut(s) 1412
BseNI ACTGG 3 cut(s) 227, 432, 1529
BseXI GCAGC 9 cut(s) 155, 158, 304, 390, 465, 537, 834, 1196, 1263
BseYI CCCAGC 1 cut(s) 1234
Bsh1236I CGCG 1 cut(s) 1096
Bsh1285I CGRYCG 1 cut(s) 621
BshFI GGCC 2 cut(s) 1379, 1535
BshNI GGYRCC 1 cut(s) 29
BshVI ATCGAT 1 cut(s) 605
BsiEI CGRYCG 1 cut(s) 621
BsiHKCI CYCGRG 1 cut(s) 303
BsiSI CCGG 1 cut(s) 304
BslFI GGGAC 1 cut(s) 1340
BslI CCNNNNNNNGG 1 cut(s) 854
BsmAI GTCTC 1 cut(s) 253
BsmFI GGGAC 1 cut(s) 1340
BsmI GAATGC 1 cut(s) 296
BsnI GGCC 2 cut(s) 1379, 1535
Bso31I GGTCTC 1 cut(s) 253
BsoBI CYCGRG 1 cut(s) 303
Bsp1407I TGTACA 1 cut(s) 1389
Bsp143I GATC 6 cut(s) 8, 667, 1083, 1146, 1212, 1238
BspACI CCGC 3 cut(s) 387, 800, 1187
BspANI GGCC 2 cut(s) 1379, 1535
BspCNI CTCAG 1 cut(s) 1413
BspDI ATCGAT 1 cut(s) 605
BspFNI CGCG 1 cut(s) 1096
BspLI GGNNCC 2 cut(s) 31, 1011
BspPI GGATC 4 cut(s) 16, 1078, 1154, 1246
BspT107I GGYRCC 1 cut(s) 29
BspTNI GGTCTC 1 cut(s) 253
BsrDI GCAATG 2 cut(s) 681, 1179
BsrGI TGTACA 1 cut(s) 1389
BsrI ACTGG 3 cut(s) 227, 432, 1529
BssECI CCNNGG 1 cut(s) 303
BssMI GATC 6 cut(s) 8, 667, 1083, 1146, 1212, 1238
Bst4CI ACNGT 8 cut(s) 157, 164, 434, 1255, 1260, 1287, 1321, 1444
Bst6I CTCTTC 4 cut(s) 256, 432, 977, 1029
BstAPI GCANNNNNTGC 2 cut(s) 36, 314
BstAUI TGTACA 1 cut(s) 1389
BstBAI YACGTR 2 cut(s) 314, 865
BstC8I GCNNGC 2 cut(s) 408, 832
BstDEI CTNAG 3 cut(s) 196, 1421, 1541
BstF5I GGATG 5 cut(s) 69, 179, 591, 1146, 1371
BstFNI CGCG 1 cut(s) 1096
BstH2I RGCGCY 1 cut(s) 1035
BstHHI GCGC 1 cut(s) 1034
BstKTI GATC 6 cut(s) 11, 670, 1086, 1149, 1215, 1241
BstMAI GTCTC 1 cut(s) 253
BstMBI GATC 6 cut(s) 8, 667, 1083, 1146, 1212, 1238
BstMCI CGRYCG 1 cut(s) 621
BstMWI GCNNNNNNNGC 7 cut(s) 36, 314, 326, 416, 525, 831, 892
BstSCI CCNGG 2 cut(s) 302, 303
BstUI CGCG 1 cut(s) 1096
BstV1I GCAGC 9 cut(s) 155, 158, 304, 390, 465, 537, 834, 1196, 1263
BstX2I RGATCY 1 cut(s) 1238
BstXI CCANNNNNNTGG 1 cut(s) 244
BstYI RGATCY 1 cut(s) 1238
Bsu15I ATCGAT 1 cut(s) 605
BsuI GTATCC 1 cut(s) 383
BsuRI GGCC 2 cut(s) 1379, 1535
BsuTUI ATCGAT 1 cut(s) 605
BtgZI GCGATG 2 cut(s) 384, 1165
BtsCI GGATG 5 cut(s) 69, 179, 591, 1146, 1371
BtsI GCAGTG 2 cut(s) 526, 936
BtsIMutI CAGTG 3 cut(s) 503, 526, 936
Cac8I GCNNGC 2 cut(s) 408, 832
CfoI GCGC 1 cut(s) 1034
Cfr9I CCCGGG 1 cut(s) 303
ClaI ATCGAT 1 cut(s) 605
CseI GACGC 1 cut(s) 1085
Csp6I GTAC 5 cut(s) 364, 1256, 1288, 1390, 1425
CviAII CATG 7 cut(s) 184, 532, 547, 598, 973, 1495, 1594
CviQI GTAC 5 cut(s) 364, 1256, 1288, 1390, 1425
DdeI CTNAG 3 cut(s) 196, 1421, 1541
DpnI GATC 6 cut(s) 10, 669, 1085, 1148, 1214, 1240
DpnII GATC 6 cut(s) 8, 667, 1083, 1146, 1212, 1238
DraIII CACNNNGTG 1 cut(s) 1544
Eam1104I CTCTTC 4 cut(s) 256, 432, 977, 1029
EarI CTCTTC 4 cut(s) 256, 432, 977, 1029
EciI GGCGGA 1 cut(s) 402
Eco31I GGTCTC 1 cut(s) 253
Eco32I GATATC 2 cut(s) 233, 603
Eco47III AGCGCT 1 cut(s) 1033
Eco57I CTGAAG 1 cut(s) 1371
Eco72I CACGTG 1 cut(s) 314
Eco88I CYCGRG 1 cut(s) 303
EcoRV GATATC 2 cut(s) 233, 603
FaeI CATG 7 cut(s) 187, 535, 550, 601, 976, 1498, 1597
FalI AAGNNNNNCTT 4 cut(s) 429, 461, 1494, 1526
FaqI GGGAC 1 cut(s) 1340
FatI CATG 7 cut(s) 183, 531, 546, 597, 972, 1494, 1593
FbaI TGATCA 1 cut(s) 667
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 9 cut(s) 144, 147, 318, 404, 454, 526, 823, 1185, 1277
FokI GGATG 5 cut(s) 76, 166, 578, 1153, 1378
Fsp4HI GCNGC 9 cut(s) 144, 147, 318, 404, 454, 526, 823, 1185, 1277
FspBI CTAG 8 cut(s) 101, 377, 450, 782, 1298, 1395, 1506, 1578
GlaI GCGC 1 cut(s) 1033
GluI GCNGC 9 cut(s) 144, 147, 318, 404, 454, 526, 823, 1185, 1277
GsaI CCCAGC 1 cut(s) 1238
HaeII RGCGCY 1 cut(s) 1035
HaeIII GGCC 2 cut(s) 1379, 1535
HapII CCGG 1 cut(s) 304
HgaI GACGC 1 cut(s) 1085
HhaI GCGC 1 cut(s) 1034
Hin1II CATG 7 cut(s) 187, 535, 550, 601, 976, 1498, 1597
Hin6I GCGC 1 cut(s) 1032
HinP1I GCGC 1 cut(s) 1032
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 1 cut(s) 832
HinfI GANTC 6 cut(s) 24, 97, 215, 645, 747, 844
HpaII CCGG 1 cut(s) 304
HphI GGTGA 5 cut(s) 136, 292, 446, 952, 1309
Hpy166II GTNNAC 4 cut(s) 160, 177, 214, 1392
Hpy188I TCNGA 5 cut(s) 48, 1151, 1309, 1351, 1489
Hpy188III TCNNGA 7 cut(s) 12, 560, 791, 1057, 1087, 1210, 1464
Hpy8I GTNNAC 4 cut(s) 160, 177, 214, 1392
HpyAV CCTTC 3 cut(s) 1356, 1464, 1591
HpyCH4III ACNGT 8 cut(s) 157, 164, 434, 1255, 1260, 1287, 1321, 1444
HpyCH4IV ACGT 3 cut(s) 313, 864, 1106
HpyF10VI GCNNNNNNNGC 7 cut(s) 36, 314, 326, 416, 525, 831, 892
HpyF3I CTNAG 3 cut(s) 196, 1421, 1541
HpySE526I ACGT 3 cut(s) 313, 864, 1106
Hsp92II CATG 7 cut(s) 187, 535, 550, 601, 976, 1498, 1597
HspAI GCGC 1 cut(s) 1032
Ksp22I TGATCA 1 cut(s) 667
Kzo9I GATC 6 cut(s) 8, 667, 1083, 1146, 1212, 1238
LmnI GCTCC 2 cut(s) 192, 416
Lsp1109I GCAGC 9 cut(s) 155, 158, 304, 390, 465, 537, 834, 1196, 1263
LweI GCATC 4 cut(s) 101, 1050, 1122, 1275
MaeI CTAG 8 cut(s) 101, 377, 450, 782, 1298, 1395, 1506, 1578
MaeII ACGT 3 cut(s) 313, 864, 1106
MaeIII GTNAC 7 cut(s) 103, 538, 772, 1102, 1415, 1453, 1583
MalI GATC 6 cut(s) 10, 669, 1085, 1148, 1214, 1240
MboI GATC 6 cut(s) 8, 667, 1083, 1146, 1212, 1238
MboII GAAGA 6 cut(s) 35, 243, 449, 994, 1016, 1286
MflI RGATCY 1 cut(s) 1238
MluCI AATT 2 cut(s) 337, 1569
MluI ACGCGT 1 cut(s) 1094
MlyI GAGTC 2 cut(s) 18, 209
MmeI TCCRAC 4 cut(s) 554, 964, 974, 1306
MseI TTAA 3 cut(s) 113, 728, 734
MslI CAYNNNNRTG 6 cut(s) 182, 545, 596, 977, 1172, 1592
MspA1I CMGCKG 1 cut(s) 1187
MspI CCGG 1 cut(s) 304
MspR9I CCNGG 2 cut(s) 304, 305
Mva1269I GAATGC 1 cut(s) 296
MvnI CGCG 1 cut(s) 1096
MwoI GCNNNNNNNGC 7 cut(s) 36, 314, 326, 416, 525, 831, 892
NciI CCSGG 2 cut(s) 304, 305
NdeII GATC 6 cut(s) 8, 667, 1083, 1146, 1212, 1238
NlaIII CATG 7 cut(s) 187, 535, 550, 601, 976, 1498, 1597
NlaIV GGNNCC 2 cut(s) 31, 1011
NmeAIII GCCGAG 2 cut(s) 6, 119
NmuCI GTSAC 2 cut(s) 103, 538
PcsI WCGNNNNNNNCGW 1 cut(s) 1155
PctI GAATGC 1 cut(s) 296
PfeI GAWTC 4 cut(s) 97, 645, 747, 844
PkrI GCNGC 9 cut(s) 145, 148, 319, 405, 455, 527, 824, 1186, 1278
PleI GAGTC 2 cut(s) 18, 209
PmaCI CACGTG 1 cut(s) 314
PmlI CACGTG 1 cut(s) 314
PpsI GAGTC 2 cut(s) 18, 209
Ppu21I YACGTR 2 cut(s) 314, 865
PsiI TTATAA 1 cut(s) 684
PspCI CACGTG 1 cut(s) 314
PspFI CCCAGC 1 cut(s) 1234
PspN4I GGNNCC 2 cut(s) 31, 1011
PsuI RGATCY 1 cut(s) 1238
RsaI GTAC 5 cut(s) 365, 1257, 1289, 1391, 1426
RsaNI GTAC 5 cut(s) 364, 1256, 1288, 1390, 1425
RseI CAYNNNNRTG 6 cut(s) 182, 545, 596, 977, 1172, 1592
SalI GTCGAC 1 cut(s) 212
SaqAI TTAA 3 cut(s) 113, 728, 734
SatI GCNGC 9 cut(s) 144, 147, 318, 404, 454, 526, 823, 1185, 1277
Sau3AI GATC 6 cut(s) 8, 667, 1083, 1146, 1212, 1238
SchI GAGTC 2 cut(s) 18, 209
ScrFI CCNGG 2 cut(s) 304, 305
SfaNI GCATC 4 cut(s) 101, 1050, 1122, 1275
SmaI CCCGGG 1 cut(s) 305
SmiMI CAYNNNNRTG 6 cut(s) 182, 545, 596, 977, 1172, 1592
SmlI CTYRAG 1 cut(s) 1208
SmoI CTYRAG 1 cut(s) 1208
Sse9I AATT 2 cut(s) 337, 1569
SsiI CCGC 3 cut(s) 387, 800, 1187
SspMI CTAG 8 cut(s) 101, 377, 450, 782, 1298, 1395, 1506, 1578
StyD4I CCNGG 2 cut(s) 302, 303
TaaI ACNGT 8 cut(s) 157, 164, 434, 1255, 1260, 1287, 1321, 1444
TaiI ACGT 3 cut(s) 316, 867, 1109
TaqI TCGA 3 cut(s) 95, 213, 605
TaqII GACCGA 2 cut(s) 607, 1153
TasI AATT 2 cut(s) 337, 1569
TatI WGTACW 3 cut(s) 363, 1287, 1389
TfiI GAWTC 4 cut(s) 97, 645, 747, 844
Tru1I TTAA 3 cut(s) 113, 728, 734
Tru9I TTAA 3 cut(s) 113, 728, 734
TscAI CASTG 3 cut(s) 503, 526, 936
TseFI GTSAC 2 cut(s) 103, 538
TseI GCWGC 9 cut(s) 143, 146, 317, 403, 453, 525, 822, 1184, 1276
Tsp45I GTSAC 2 cut(s) 103, 538
TspDTI ATGAA 9 cut(s) 330, 752, 777, 995, 1128, 1511, 1515, 1610, 1632
TspGWI ACGGA 1 cut(s) 1153
TspMI CCCGGG 1 cut(s) 303
TspRI CASTG 3 cut(s) 503, 526, 936
XcmI CCANNNNNNNNNTGG 1 cut(s) 604
XmaI CCCGGG 1 cut(s) 303
XmiI GTMKAC 1 cut(s) 213
XspI CTAG 8 cut(s) 101, 377, 450, 782, 1298, 1395, 1506, 1578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.