FvH4_6g50271

RNase H

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010670
Physical Location & Seq
Reverse (-)
37427166 .. 37430152
2987 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g50271.t1

Sequence Viewer

Length: 933 bp
ATGGAGGAAGAGAAGGATGCCTTTTATGTTGTTCGAAAGGGAGATGTGGTTGGCATATATAAAAGCTTGAAGGATTGCCAAAACCAAGCTGGTTCATCGGTATGCAATCCTTCTGTAAGTGTGTTTAAAGGGTATGGTTTGCCTAAGGAGGCCGAGGAGTACCTTGTCTCGCATGGGCTTAAGAATGCTTCATATACTATCAGTGCCAGTGATGTGAAAGATGGTCTGTTTGGAAGCCTTGTTGCTTGTCCTTACCAGCAGCCAGCATCTTCTATGGTTAATTCAGGGTTCAAAATTGCACCTGATCAGCTTACACGAAAGAGAATGCCGGATGTGATAAATTCAGGTGTCAATGACCCTCCACAAAAGAGATCACTGGATCAGCCAGCGTCTTCTATGGTTAATTCTGGCTTCAAAATTGCACCTCATCAGCTTACGCGAATGAGATTGCCGGATGTGGTAAATTCAGGTGTCAATGACCCTCCACAGAGGACATTGCCGGATCAATCCTGCATACTTGAGTTTGATGGAGCTTCAAAAGGAAATCCTGGATTATCTGGTGCAGGAGCTGTACTTCGTGCTGAAGATGGGAGTGTTTTGCACCGGCTGCGGGAAGGCGTGGGCATTGCAACAAATAACGTCGCTGAATATCGAGCTGTAATTCTAGGATTGAAATATGCTCTTGAGAAAGGTTATAAGCACATTCGTGTTCAGGGAGACTCTAAACTTGTCTGCTGCCAGATTCAGGGGACCTGGAAGACAAAAAATCAGAATATGGCCGACCTGTGCCAAGTGGCCAAGCAGCTTAAGGACAAGTTTACTTCTTTTCAGATCAATCATGTTCTCAGGGAGTTCAATGCAGATGCTGATGTTCTAGCAAACCGAGCAGTAAATCTCAGAGATGGTCAAGTTGAAGTGGAATCCCAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cauli_VI RVT_3

Protein Analysis

311

Amino Acids

33.79

Weight (kDa)

8.37

Isoelectric Point (pI)

31.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_H PF00075 173 - 294 6.3e-10 RNase H
RVT_3 PF13456 175 - 296 3.9e-27 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 696
AccII CGCG 1 cut(s) 439
AciI CCGC 1 cut(s) 610
AclWI GGATC 2 cut(s) 387, 510
AcoI YGGCCR 2 cut(s) 777, 795
AcsI RAATTY 2 cut(s) 340, 463
AcuI CTGAAG 1 cut(s) 603
AfaI GTAC 2 cut(s) 161, 573
AfiI CCNNNNNNNGG 2 cut(s) 610, 745
AflII CTTAAG 2 cut(s) 179, 806
AgsI TTSAA 7 cut(s) 70, 292, 415, 537, 673, 856, 914
AjnI CCWGG 2 cut(s) 547, 752
AleI CACNNNNGTG 1 cut(s) 705
AluBI AGCT 8 cut(s) 66, 89, 310, 433, 533, 569, 656, 805
AluI AGCT 8 cut(s) 66, 89, 310, 433, 533, 569, 656, 805
Alw26I GTCTC 2 cut(s) 172, 711
AlwI GGATC 2 cut(s) 387, 510
AlwNI CAGNNNCTG 2 cut(s) 569, 866
AoxI GGCC 3 cut(s) 150, 777, 795
ApeKI GCWGC 4 cut(s) 259, 607, 735, 802
ApoI RAATTY 2 cut(s) 340, 463
AspS9I GGNCC 1 cut(s) 750
AsuII TTCGAA 1 cut(s) 34
AvaII GGWCC 1 cut(s) 750
AxyI CCTNAGG 1 cut(s) 144
BalI TGGCCA 1 cut(s) 797
BbsI GAAGAC 2 cut(s) 384, 764
BbvI GCAGC 4 cut(s) 271, 594, 722, 814
BccI CCATC 4 cut(s) 215, 521, 581, 896
BciT130I CCWGG 2 cut(s) 549, 754
BclI TGATCA 1 cut(s) 304
BcoDI GTCTC 2 cut(s) 172, 711
BfaI CTAG 2 cut(s) 665, 875
BfrI CTTAAG 2 cut(s) 179, 806
BisI GCNGC 4 cut(s) 260, 608, 736, 803
BlsI GCNGC 4 cut(s) 261, 609, 737, 804
Bme1390I CCNGG 2 cut(s) 549, 754
Bme18I GGWCC 1 cut(s) 750
BmgT120I GGNCC 1 cut(s) 750
BmiI GGNNCC 1 cut(s) 751
BmrFI CCNGG 2 cut(s) 549, 754
BmsI GCATC 3 cut(s) 7, 275, 853
BpiI GAAGAC 2 cut(s) 384, 764
Bpu14I TTCGAA 1 cut(s) 34
BpuEI CTTGAG 2 cut(s) 539, 704
BsaJI CCNNGG 1 cut(s) 153
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 2 cut(s) 610, 745
Bse118I RCCGGY 1 cut(s) 603
Bse1I ACTGG 2 cut(s) 207, 381
Bse21I CCTNAGG 1 cut(s) 144
Bse3DI GCAATG 2 cut(s) 494, 624
BseBI CCWGG 2 cut(s) 549, 754
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 3 cut(s) 22, 337, 460
BseLI CCNNNNNNNGG 2 cut(s) 610, 745
BseMI GCAATG 2 cut(s) 494, 624
BseMII CTCAG 2 cut(s) 859, 910
BseNI ACTGG 2 cut(s) 207, 381
BseRI GAGGAG 1 cut(s) 170
BseXI GCAGC 4 cut(s) 271, 594, 722, 814
BseYI CCCAGC 1 cut(s) 924
BsgI GTGCAG 1 cut(s) 582
Bsh1236I CGCG 1 cut(s) 439
BshFI GGCC 3 cut(s) 152, 779, 797
BsiSI CCGG 4 cut(s) 329, 452, 500, 604
BslFI GGGAC 1 cut(s) 763
BslI CCNNNNNNNGG 2 cut(s) 610, 745
BsmAI GTCTC 2 cut(s) 172, 711
BsmFI GGGAC 1 cut(s) 763
BsmI GAATGC 2 cut(s) 190, 330
BsnI GGCC 3 cut(s) 152, 779, 797
Bsp119I TTCGAA 1 cut(s) 34
Bsp143I GATC 5 cut(s) 304, 371, 379, 502, 831
BspACI CCGC 1 cut(s) 610
BspANI GGCC 3 cut(s) 152, 779, 797
BspCNI CTCAG 2 cut(s) 858, 909
BspFNI CGCG 1 cut(s) 439
BspLI GGNNCC 1 cut(s) 751
BspPI GGATC 2 cut(s) 387, 510
BspT104I TTCGAA 1 cut(s) 34
BspTI CTTAAG 2 cut(s) 179, 806
BsrDI GCAATG 2 cut(s) 494, 624
BsrFI RCCGGY 1 cut(s) 603
BsrI ACTGG 2 cut(s) 207, 381
BssAI RCCGGY 1 cut(s) 603
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 5 cut(s) 304, 371, 379, 502, 831
Bst2UI CCWGG 2 cut(s) 549, 754
Bst6I CTCTTC 1 cut(s) 3
BstAFI CTTAAG 2 cut(s) 179, 806
BstAPI GCANNNNNTGC 1 cut(s) 607
BstBI TTCGAA 1 cut(s) 34
BstC8I GCNNGC 2 cut(s) 264, 387
BstDEI CTNAG 3 cut(s) 144, 845, 896
BstF5I GGATG 3 cut(s) 22, 337, 460
BstFNI CGCG 1 cut(s) 439
BstKTI GATC 5 cut(s) 307, 374, 382, 505, 834
BstMAI GTCTC 2 cut(s) 172, 711
BstMBI GATC 5 cut(s) 304, 371, 379, 502, 831
BstMWI GCNNNNNNNGC 2 cut(s) 607, 884
BstNI CCWGG 2 cut(s) 549, 754
BstSCI CCNGG 2 cut(s) 547, 752
BstUI CGCG 1 cut(s) 439
BstV1I GCAGC 4 cut(s) 271, 594, 722, 814
BstV2I GAAGAC 2 cut(s) 384, 764
Bsu36I CCTNAGG 1 cut(s) 144
BsuRI GGCC 3 cut(s) 152, 779, 797
BtsCI GGATG 3 cut(s) 22, 337, 460
BtsIMutI CAGTG 3 cut(s) 208, 214, 374
Cac8I GCNNGC 2 cut(s) 264, 387
CaiI CAGNNNCTG 2 cut(s) 569, 866
Cfr10I RCCGGY 1 cut(s) 603
Cfr13I GGNCC 1 cut(s) 750
CseI GACGC 1 cut(s) 378
Csp6I GTAC 2 cut(s) 160, 572
CviAII CATG 2 cut(s) 173, 839
CviQI GTAC 2 cut(s) 160, 572
DdeI CTNAG 3 cut(s) 144, 845, 896
DpnI GATC 5 cut(s) 306, 373, 381, 504, 833
DpnII GATC 5 cut(s) 304, 371, 379, 502, 831
DraI TTTAAA 1 cut(s) 127
EaeI YGGCCR 2 cut(s) 777, 795
Eam1104I CTCTTC 1 cut(s) 3
EarI CTCTTC 1 cut(s) 3
Eco47I GGWCC 1 cut(s) 750
Eco57I CTGAAG 1 cut(s) 603
Eco81I CCTNAGG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 750
EcoRII CCWGG 2 cut(s) 547, 752
FaeI CATG 2 cut(s) 176, 842
FalI AAGNNNNNCTT 1 cut(s) 37
FaqI GGGAC 1 cut(s) 763
FatI CATG 2 cut(s) 172, 838
FauI CCCGC 1 cut(s) 603
FbaI TGATCA 1 cut(s) 304
Fnu4HI GCNGC 4 cut(s) 260, 608, 736, 803
FokI GGATG 3 cut(s) 29, 344, 467
Fsp4HI GCNGC 4 cut(s) 260, 608, 736, 803
FspBI CTAG 2 cut(s) 665, 875
GluI GCNGC 4 cut(s) 260, 608, 736, 803
GsaI CCCAGC 1 cut(s) 928
HaeIII GGCC 3 cut(s) 152, 779, 797
HapII CCGG 4 cut(s) 329, 452, 500, 604
HgaI GACGC 1 cut(s) 378
Hin1II CATG 2 cut(s) 176, 842
HindIII AAGCTT 1 cut(s) 64
HinfI GANTC 3 cut(s) 719, 742, 920
HpaII CCGG 4 cut(s) 329, 452, 500, 604
Hpy166II GTNNAC 1 cut(s) 819
Hpy188I TCNGA 3 cut(s) 771, 831, 899
Hpy188III TCNNGA 1 cut(s) 683
Hpy8I GTNNAC 1 cut(s) 819
Hpy99I CGWCG 1 cut(s) 644
HpyAV CCTTC 4 cut(s) 7, 64, 120, 608
HpyCH4IV ACGT 1 cut(s) 639
HpyCH4V TGCA 8 cut(s) 105, 299, 422, 513, 563, 601, 629, 860
HpyF10VI GCNNNNNNNGC 2 cut(s) 607, 884
HpyF3I CTNAG 3 cut(s) 144, 845, 896
HpySE526I ACGT 1 cut(s) 639
Hsp92II CATG 2 cut(s) 176, 842
Ksp22I TGATCA 1 cut(s) 304
Kzo9I GATC 5 cut(s) 304, 371, 379, 502, 831
LmnI GCTCC 2 cut(s) 530, 566
Lsp1109I GCAGC 4 cut(s) 271, 594, 722, 814
LweI GCATC 3 cut(s) 7, 275, 853
MaeI CTAG 2 cut(s) 665, 875
MaeII ACGT 1 cut(s) 639
MalI GATC 5 cut(s) 306, 373, 381, 504, 833
MboI GATC 5 cut(s) 304, 371, 379, 502, 831
MboII GAAGA 5 cut(s) 20, 261, 384, 596, 769
MlsI TGGCCA 1 cut(s) 797
MluCI AATT 7 cut(s) 280, 294, 340, 403, 417, 463, 660
MluNI TGGCCA 1 cut(s) 797
MlyI GAGTC 1 cut(s) 713
MnlI CCTC 6 cut(s) 142, 148, 369, 435, 483, 492
Mox20I TGGCCA 1 cut(s) 797
MscI TGGCCA 1 cut(s) 797
MseI TTAA 5 cut(s) 126, 180, 279, 402, 807
MslI CAYNNNNRTG 2 cut(s) 100, 705
Msp20I TGGCCA 1 cut(s) 797
MspCI CTTAAG 2 cut(s) 179, 806
MspI CCGG 4 cut(s) 329, 452, 500, 604
MspR9I CCNGG 2 cut(s) 549, 754
Mva1269I GAATGC 2 cut(s) 190, 330
MvaI CCWGG 2 cut(s) 549, 754
MvnI CGCG 1 cut(s) 439
MwoI GCNNNNNNNGC 2 cut(s) 607, 884
NdeII GATC 5 cut(s) 304, 371, 379, 502, 831
NlaIII CATG 2 cut(s) 176, 842
NlaIV GGNNCC 1 cut(s) 751
NmeAIII GCCGAG 1 cut(s) 178
NspV TTCGAA 1 cut(s) 34
OliI CACNNNNGTG 1 cut(s) 705
PctI GAATGC 2 cut(s) 190, 330
PfeI GAWTC 2 cut(s) 742, 920
PfoI TCCNGGA 1 cut(s) 547
PkrI GCNGC 4 cut(s) 261, 609, 737, 804
PleI GAGTC 1 cut(s) 713
PpsI GAGTC 1 cut(s) 713
PpuMI RGGWCCY 1 cut(s) 750
PsiI TTATAA 1 cut(s) 696
Psp5II RGGWCCY 1 cut(s) 750
Psp6I CCWGG 2 cut(s) 547, 752
PspFI CCCAGC 1 cut(s) 924
PspGI CCWGG 2 cut(s) 547, 752
PspN4I GGNNCC 1 cut(s) 751
PspPI GGNCC 1 cut(s) 750
PspPPI RGGWCCY 1 cut(s) 750
PstNI CAGNNNCTG 2 cut(s) 569, 866
RsaI GTAC 2 cut(s) 161, 573
RsaNI GTAC 2 cut(s) 160, 572
RseI CAYNNNNRTG 2 cut(s) 100, 705
SaqAI TTAA 5 cut(s) 126, 180, 279, 402, 807
SatI GCNGC 4 cut(s) 260, 608, 736, 803
Sau3AI GATC 5 cut(s) 304, 371, 379, 502, 831
Sau96I GGNCC 1 cut(s) 750
SchI GAGTC 1 cut(s) 713
ScrFI CCNGG 2 cut(s) 549, 754
SfaNI GCATC 3 cut(s) 7, 275, 853
SfuI TTCGAA 1 cut(s) 34
SinI GGWCC 1 cut(s) 750
SmiMI CAYNNNNRTG 2 cut(s) 100, 705
SmlI CTYRAG 4 cut(s) 179, 518, 683, 806
SmoI CTYRAG 4 cut(s) 179, 518, 683, 806
Sse9I AATT 7 cut(s) 280, 294, 340, 403, 417, 463, 660
SsiI CCGC 1 cut(s) 610
SspMI CTAG 2 cut(s) 665, 875
StyD4I CCNGG 2 cut(s) 547, 752
TaiI ACGT 1 cut(s) 642
TaqI TCGA 2 cut(s) 34, 652
TasI AATT 7 cut(s) 280, 294, 340, 403, 417, 463, 660
TatI WGTACW 1 cut(s) 571
TfiI GAWTC 2 cut(s) 742, 920
Tru1I TTAA 5 cut(s) 126, 180, 279, 402, 807
Tru9I TTAA 5 cut(s) 126, 180, 279, 402, 807
TscAI CASTG 3 cut(s) 208, 214, 381
TseI GCWGC 4 cut(s) 259, 607, 735, 802
TspDTI ATGAA 2 cut(s) 84, 180
TspRI CASTG 3 cut(s) 208, 214, 381
Vha464I CTTAAG 2 cut(s) 179, 806
VpaK11BI GGWCC 1 cut(s) 750
XapI RAATTY 2 cut(s) 340, 463
XcmI CCANNNNNNNNNTGG 1 cut(s) 86
XspI CTAG 2 cut(s) 665, 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.