Rh2CG602400

RNase H

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0010670
Physical Location & Seq
Reverse (-)
77765734 .. 77767834
2101 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG602400.1

Sequence Viewer

Length: 939 bp
ATGGAGGAAGAGAAGGATGCTTTTTATGTTGTTCGAAAGGGAGATGTGGTGGGCATATACAAAAGTTTGAAGGATTGTCAAAACCAAGCCGGTTCCTCGGTATGCAATCCTTCTGTAAGTGTGTTTAAAGGGTATGGTTTGCCTAAGGAGGCCGAGGAGTACCTGGTTTCACACGGGCTTAAGAATGCTTCATATACAATCAGTGCCAGTGACGTGAAAGTTGATCTGTTTGGAAGCCTTGTTGCTTGTCCTTACCAGCAGCCGGCATCTTCTATGGTGAATTCAGGTGTCGAAGACCCTTCAAAAAGGAGATTTCCAGATATGGCGCTTTCCTCGGCTTCCAGTTTCTCTCCACATAAGCATTTTAAAGCTGATAGTTGTAGTCAAACTCGATTAATTTCTTCTACCAGTCAATGCACACTTGAGTTTGATGGTGCTTCAAAAGGAAATCCAGGACTATCTGGTGCGGGAGCTGTACTCCGTGCTGAAGACGGGAGTGTTTTGTACTGGCTGCGGGAAGGGGTGGGCATTGCAACAAATAACGTTGCTGAATATCGAGCTGTAATTCTAGGATTGAAGTATGCTCTTGAGAAAGGATATAAGCACATTCGTGTTAAAGGAGACTCTAAACTTGTCTGCTGCCAGATTCAGGGGACCTGGAAAACAAAAAATCAGAATATGGCCGACCTGTGCCAAATCGCCAAGCAGCTTAAGGAAAAGTTTACTTCTTTTGAGATCAATCATGTTCTCAGGGAGTTCAATGCCGAGGCTGATGTTCAAGCAAACCGAGCCATAAATCTCAGAGGTGAGTGGCTTTCAGTTCAATCAGTTCTCATGCTTTTCATGGTTTATGCTTTGCTCATATTCATTAGAATTGACTGGAGATCTTATATGCTTAAGCAGATGGACAAGTTGAAGTGGAATCGGCGCATTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cauli_VI RVT_3

Protein Analysis

312

Amino Acids

34.8

Weight (kDa)

9.04

Isoelectric Point (pI)

35.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_H PF00075 140 - 249 2.8e-09 RNase H
RVT_3 PF13456 143 - 264 3.8e-27 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 467, 514
AclI AACGTT 1 cut(s) 543
AcoI YGGCCR 1 cut(s) 681
AcsI RAATTY 1 cut(s) 280
AcuI CTGAAG 1 cut(s) 507
AfaI GTAC 3 cut(s) 161, 477, 506
AfiI CCNNNNNNNGG 2 cut(s) 262, 649
AflII CTTAAG 3 cut(s) 179, 710, 896
AgsI TTSAA 8 cut(s) 70, 303, 441, 577, 760, 779, 824, 916
AjiI CACGTC 1 cut(s) 214
AjnI CCWGG 3 cut(s) 162, 451, 656
AleI CACNNNNGTG 1 cut(s) 609
AluBI AGCT 4 cut(s) 371, 473, 560, 709
AluI AGCT 4 cut(s) 371, 473, 560, 709
Alw26I GTCTC 1 cut(s) 615
AoxI GGCC 2 cut(s) 150, 681
ApeKI GCWGC 4 cut(s) 259, 511, 639, 706
ApoI RAATTY 1 cut(s) 280
AseI ATTAAT 1 cut(s) 395
AspLEI GCGC 2 cut(s) 328, 930
AspS9I GGNCC 1 cut(s) 654
AsuHPI GGTGA 2 cut(s) 289, 818
AsuII TTCGAA 1 cut(s) 34
AvaII GGWCC 1 cut(s) 654
AxyI CCTNAGG 1 cut(s) 144
BbsI GAAGAC 2 cut(s) 300, 495
BbvI GCAGC 4 cut(s) 271, 498, 626, 718
BccI CCATC 2 cut(s) 425, 898
BciT130I CCWGG 3 cut(s) 164, 453, 658
BcoDI GTCTC 1 cut(s) 615
BfaI CTAG 1 cut(s) 569
BfoI RGCGCY 1 cut(s) 329
BfrI CTTAAG 3 cut(s) 179, 710, 896
BglII AGATCT 1 cut(s) 884
BisI GCNGC 4 cut(s) 260, 512, 640, 707
BlsI GCNGC 4 cut(s) 261, 513, 641, 708
Bme1390I CCNGG 3 cut(s) 164, 453, 658
Bme18I GGWCC 1 cut(s) 654
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 654
BmiI GGNNCC 2 cut(s) 94, 655
BmrFI CCNGG 3 cut(s) 164, 453, 658
BmsI GCATC 2 cut(s) 7, 275
BpiI GAAGAC 2 cut(s) 300, 495
BplI GAGNNNNNCTC 2 cut(s) 462, 494
BpmI CTGGAG 1 cut(s) 901
Bpu14I TTCGAA 1 cut(s) 34
BpuEI CTTGAG 2 cut(s) 443, 608
BsaJI CCNNGG 4 cut(s) 96, 153, 333, 765
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 649
Bse118I RCCGGY 2 cut(s) 89, 262
Bse1I ACTGG 5 cut(s) 207, 342, 408, 512, 884
Bse21I CCTNAGG 1 cut(s) 144
Bse3DI GCAATG 1 cut(s) 528
BseBI CCWGG 3 cut(s) 164, 453, 658
BseDI CCNNGG 4 cut(s) 96, 153, 333, 765
BseGI GGATG 1 cut(s) 22
BseLI CCNNNNNNNGG 2 cut(s) 262, 649
BseMI GCAATG 1 cut(s) 528
BseMII CTCAG 2 cut(s) 763, 814
BseNI ACTGG 5 cut(s) 207, 342, 408, 512, 884
BseRI GAGGAG 1 cut(s) 170
BseXI GCAGC 4 cut(s) 271, 498, 626, 718
BshFI GGCC 2 cut(s) 152, 683
BsiSI CCGG 2 cut(s) 90, 263
BslFI GGGAC 1 cut(s) 667
BslI CCNNNNNNNGG 2 cut(s) 262, 649
BsmAI GTCTC 1 cut(s) 615
BsmFI GGGAC 1 cut(s) 667
BsmI GAATGC 1 cut(s) 190
BsnI GGCC 2 cut(s) 152, 683
Bsp119I TTCGAA 1 cut(s) 34
Bsp143I GATC 3 cut(s) 223, 735, 884
BspACI CCGC 2 cut(s) 467, 514
BspANI GGCC 2 cut(s) 152, 683
BspCNI CTCAG 2 cut(s) 762, 813
BspLI GGNNCC 2 cut(s) 94, 655
BspT104I TTCGAA 1 cut(s) 34
BspTI CTTAAG 3 cut(s) 179, 710, 896
BsrDI GCAATG 1 cut(s) 528
BsrFI RCCGGY 2 cut(s) 89, 262
BsrI ACTGG 5 cut(s) 207, 342, 408, 512, 884
BssAI RCCGGY 2 cut(s) 89, 262
BssECI CCNNGG 4 cut(s) 96, 153, 333, 765
BssMI GATC 3 cut(s) 223, 735, 884
Bst2UI CCWGG 3 cut(s) 164, 453, 658
Bst6I CTCTTC 1 cut(s) 3
BstAFI CTTAAG 3 cut(s) 179, 710, 896
BstBI TTCGAA 1 cut(s) 34
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 3 cut(s) 144, 749, 800
BstF5I GGATG 1 cut(s) 22
BstH2I RGCGCY 1 cut(s) 329
BstHHI GCGC 2 cut(s) 328, 930
BstKTI GATC 3 cut(s) 226, 738, 887
BstMAI GTCTC 1 cut(s) 615
BstMBI GATC 3 cut(s) 223, 735, 884
BstMWI GCNNNNNNNGC 1 cut(s) 788
BstNI CCWGG 3 cut(s) 164, 453, 658
BstSCI CCNGG 3 cut(s) 162, 451, 656
BstV1I GCAGC 4 cut(s) 271, 498, 626, 718
BstV2I GAAGAC 2 cut(s) 300, 495
BstX2I RGATCY 1 cut(s) 884
BstYI RGATCY 1 cut(s) 884
Bsu36I CCTNAGG 1 cut(s) 144
BsuRI GGCC 2 cut(s) 152, 683
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 1 cut(s) 22
BtsIMutI CAGTG 2 cut(s) 208, 214
Cac8I GCNNGC 1 cut(s) 264
CfoI GCGC 2 cut(s) 328, 930
Cfr10I RCCGGY 2 cut(s) 89, 262
Cfr13I GGNCC 1 cut(s) 654
CsiI ACCWGGT 1 cut(s) 162
Csp6I GTAC 3 cut(s) 160, 476, 505
CviAII CATG 3 cut(s) 743, 835, 844
CviQI GTAC 3 cut(s) 160, 476, 505
DdeI CTNAG 3 cut(s) 144, 749, 800
DpnI GATC 3 cut(s) 225, 737, 886
DpnII GATC 3 cut(s) 223, 735, 884
DraI TTTAAA 2 cut(s) 127, 367
EaeI YGGCCR 1 cut(s) 681
Eam1104I CTCTTC 1 cut(s) 3
EarI CTCTTC 1 cut(s) 3
Eco47I GGWCC 1 cut(s) 654
Eco57I CTGAAG 1 cut(s) 507
Eco81I CCTNAGG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 654
EcoRI GAATTC 1 cut(s) 280
EcoRII CCWGG 3 cut(s) 162, 451, 656
FaeI CATG 3 cut(s) 746, 838, 847
FaqI GGGAC 1 cut(s) 667
FatI CATG 3 cut(s) 742, 834, 843
FauI CCCGC 2 cut(s) 460, 507
Fnu4HI GCNGC 4 cut(s) 260, 512, 640, 707
FokI GGATG 1 cut(s) 29
Fsp4HI GCNGC 4 cut(s) 260, 512, 640, 707
FspBI CTAG 1 cut(s) 569
GlaI GCGC 2 cut(s) 327, 929
GluI GCNGC 4 cut(s) 260, 512, 640, 707
GsuI CTGGAG 1 cut(s) 901
HaeII RGCGCY 1 cut(s) 329
HaeIII GGCC 2 cut(s) 152, 683
HapII CCGG 2 cut(s) 90, 263
HhaI GCGC 2 cut(s) 328, 930
Hin1II CATG 3 cut(s) 746, 838, 847
Hin6I GCGC 2 cut(s) 326, 928
HinP1I GCGC 2 cut(s) 326, 928
HinfI GANTC 3 cut(s) 623, 646, 922
HpaII CCGG 2 cut(s) 90, 263
HphI GGTGA 2 cut(s) 289, 818
Hpy166II GTNNAC 1 cut(s) 723
Hpy188I TCNGA 2 cut(s) 675, 803
Hpy188III TCNNGA 2 cut(s) 317, 587
Hpy8I GTNNAC 1 cut(s) 723
HpyAV CCTTC 5 cut(s) 7, 64, 120, 309, 512
HpyCH4IV ACGT 2 cut(s) 213, 543
HpyCH4V TGCA 3 cut(s) 105, 417, 533
HpyF10VI GCNNNNNNNGC 1 cut(s) 788
HpyF3I CTNAG 3 cut(s) 144, 749, 800
HpySE526I ACGT 2 cut(s) 213, 543
Hsp92II CATG 3 cut(s) 746, 838, 847
HspAI GCGC 2 cut(s) 326, 928
KroI GCCGGC 1 cut(s) 262
KroNI GCCGGC 1 cut(s) 264
Kzo9I GATC 3 cut(s) 223, 735, 884
LmnI GCTCC 1 cut(s) 470
Lsp1109I GCAGC 4 cut(s) 271, 498, 626, 718
LweI GCATC 2 cut(s) 7, 275
MabI ACCWGGT 1 cut(s) 162
MaeI CTAG 1 cut(s) 569
MaeII ACGT 2 cut(s) 213, 543
MaeIII GTNAC 1 cut(s) 209
MalI GATC 3 cut(s) 225, 737, 886
MboI GATC 3 cut(s) 223, 735, 884
MboII GAAGA 5 cut(s) 20, 261, 305, 393, 500
MflI RGATCY 1 cut(s) 884
MluCI AATT 4 cut(s) 280, 396, 564, 873
MlyI GAGTC 1 cut(s) 617
MnlI CCTC 6 cut(s) 106, 142, 148, 343, 760, 797
MroNI GCCGGC 1 cut(s) 262
MseI TTAA 8 cut(s) 126, 180, 366, 395, 615, 711, 897, 933
MslI CAYNNNNRTG 1 cut(s) 609
MspCI CTTAAG 3 cut(s) 179, 710, 896
MspI CCGG 2 cut(s) 90, 263
MspR9I CCNGG 3 cut(s) 164, 453, 658
Mva1269I GAATGC 1 cut(s) 190
MvaI CCWGG 3 cut(s) 164, 453, 658
MwoI GCNNNNNNNGC 1 cut(s) 788
NaeI GCCGGC 1 cut(s) 264
NdeII GATC 3 cut(s) 223, 735, 884
NgoMIV GCCGGC 1 cut(s) 262
NlaIII CATG 3 cut(s) 746, 838, 847
NlaIV GGNNCC 2 cut(s) 94, 655
NmeAIII GCCGAG 3 cut(s) 178, 314, 790
NmuCI GTSAC 1 cut(s) 209
NspV TTCGAA 1 cut(s) 34
OliI CACNNNNGTG 1 cut(s) 609
PctI GAATGC 1 cut(s) 190
PdiI GCCGGC 1 cut(s) 264
PfeI GAWTC 2 cut(s) 646, 922
PfoI TCCNGGA 1 cut(s) 451
PkrI GCNGC 4 cut(s) 261, 513, 641, 708
PleI GAGTC 1 cut(s) 617
PpsI GAGTC 1 cut(s) 617
PpuMI RGGWCCY 1 cut(s) 654
PshBI ATTAAT 1 cut(s) 395
Psp1406I AACGTT 1 cut(s) 543
Psp5II RGGWCCY 1 cut(s) 654
Psp6I CCWGG 3 cut(s) 162, 451, 656
PspGI CCWGG 3 cut(s) 162, 451, 656
PspN4I GGNNCC 2 cut(s) 94, 655
PspPI GGNCC 1 cut(s) 654
PspPPI RGGWCCY 1 cut(s) 654
PsuI RGATCY 1 cut(s) 884
RsaI GTAC 3 cut(s) 161, 477, 506
RsaNI GTAC 3 cut(s) 160, 476, 505
RseI CAYNNNNRTG 1 cut(s) 609
SaqAI TTAA 8 cut(s) 126, 180, 366, 395, 615, 711, 897, 933
SatI GCNGC 4 cut(s) 260, 512, 640, 707
Sau3AI GATC 3 cut(s) 223, 735, 884
Sau96I GGNCC 1 cut(s) 654
SchI GAGTC 1 cut(s) 617
ScrFI CCNGG 3 cut(s) 164, 453, 658
SexAI ACCWGGT 1 cut(s) 162
SfaNI GCATC 2 cut(s) 7, 275
SfuI TTCGAA 1 cut(s) 34
SinI GGWCC 1 cut(s) 654
SmiMI CAYNNNNRTG 1 cut(s) 609
SmlI CTYRAG 5 cut(s) 179, 422, 587, 710, 896
SmoI CTYRAG 5 cut(s) 179, 422, 587, 710, 896
Sse9I AATT 4 cut(s) 280, 396, 564, 873
SsiI CCGC 2 cut(s) 467, 514
SspMI CTAG 1 cut(s) 569
StyD4I CCNGG 3 cut(s) 162, 451, 656
TaiI ACGT 2 cut(s) 216, 546
TaqI TCGA 4 cut(s) 34, 291, 391, 556
TasI AATT 4 cut(s) 280, 396, 564, 873
TatI WGTACW 2 cut(s) 475, 504
TfiI GAWTC 2 cut(s) 646, 922
Tru1I TTAA 8 cut(s) 126, 180, 366, 395, 615, 711, 897, 933
Tru9I TTAA 8 cut(s) 126, 180, 366, 395, 615, 711, 897, 933
TscAI CASTG 2 cut(s) 208, 214
TseFI GTSAC 1 cut(s) 209
TseI GCWGC 4 cut(s) 259, 511, 639, 706
Tsp45I GTSAC 1 cut(s) 209
TspDTI ATGAA 3 cut(s) 180, 832, 856
TspGWI ACGGA 1 cut(s) 470
TspRI CASTG 2 cut(s) 208, 214
Vha464I CTTAAG 3 cut(s) 179, 710, 896
VpaK11BI GGWCC 1 cut(s) 654
VspI ATTAAT 1 cut(s) 395
XapI RAATTY 1 cut(s) 280
XspI CTAG 1 cut(s) 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.