FvH4_6g51050

Polyribonucleotide nucleotidyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004785
Physical Location & Seq
Forward (+)
37901319 .. 37910840
9522 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g51050.t1

Sequence Viewer

Length: 2910 bp
ATGGCCACCGCGGCGGCCCACCGCGCCAACCCGCTCCTCGGCCGCCTCCCTCATTTCCTCACATGGCGATCCCTCGGCTTCCGCACCGTCTGCAGCGGCCGCATGGGCTTCTCCTCCCAACCCGAACCCGAACCCGAATCCCATACTCGACCCGGAACCAAGCTCCTCGAAACCTTCACCGAGCAATTCGAGATCGGATCACGCTTGATCACACTCGAGACCGGAAAGATCGCTCGCTTCGCTAACGGCTCCGTCGTCTTGGGCATGGACGACACTCGTGTTCTCTCCACCGTCTGCTCCGCCAAGGGTGACGCCGTTAAGGACTTTCTACCCCTCACCGTTGATTATCAAGAGAAGCAATTCGCGCAAGGTGTTATACCAAACACATTCATGAGGAGGGAGGGTGCACCGAAAGAGCGTGAACTCTTGTGTGCTCGTATAATTGATCGGCCGATTCGTCCCTTGTTTCCTGCAGGCTTTTTCCATGAGGTTCAGGTGACGGCGAATGTTCTTTCTTCTGATGGGAAGCAAGATCCAGATGTAATGGCTGCTAATGCTACGTCTGCTGCTCTTATGTTATCAGATATTCCTTGGGCGGGCCCCATTGGAATGATTCGCATTGGGAGGATTGAGGGGAAGTTTATTGTTAACCCAACTATGGATGAGCTTAGCTTAAGTGATCTCAACTTAGTGTATGCCTGTACAAGGGACAAAACGTTAATGATTGATGTGCAAGCTCGTGAGATTTCCGAAAGAGATCTAGAAGCTGGGTTGAGATTGGCTCATCCGGAAGCAGTTAAATATATTGAACCTCAAATCAGACTGGCTGCTAAAGCTGGCAAGCATAAGAAGGAATATAAGTTGTCACTGATGCCTGAGAGAACATTGGAGAAAGTCGCGCAGTTGGCAGAAGCTCCTATAGAAGAAGTTTTCACTGATCCCAAGTATGGAAAGTTTGAACGTGGAGAAGCCTTGGAGAATATTACGCAAGATGTGAGGAAAGCACTTGAAGAAGAGTGTGATGAAGAAAGCTTGAGACTCCTACCGAAAGCAGTAGACACAGTGAGGAAAAAGGTTGTCCGTAGAAGAATAATTGCGGAAGGATCAAGACTTGATGGGAGGCGACTCGATGAAGTTAGGCCTGTGTACTGTGAATCTGGTATTTTGCCTATGTTGCATGGATCATCACTTTTTTCTCGTGGAGACACGCAGGTACTCTGTACTGTAACACTTGGAGCACCTGGTGATGCACAACGGTTGGAATCTGTGGTGGGTCCTCCATCAAAGCGCTTCATGCTTCACTATAGTTTCCCCCCATTCTGTATAAATGAGGTCGGTAAACATGGTGGTTTGAACAGGCGTGAAGTTGGTCATGGAACTCTGGCGGAAAAAGCTCTACTCGCTGTGTTACCTCCTGAAGATGAATTTCCATACACTGTCCGTATCAATTCTGAAGTAATGGCTTCTGATGGTTCAACATCTATGGCTTCTGTCTGTGGAGGCAGTATGGCTTTGATGGATGCTGGCATTCCGCTGCGCAAACATGTAGCTGGTGTCTCCGTGGGTCTTGTTAGTGAAGTTGATCAAATAACTGGCACAATAAAGGAATACTGTATATTGACTGATATTCTGGGTCTGGAAGACCATTTGGGGGACATGGACTTCAAAATTGTTGGCACTCAAGATGGAGTCACCGCAATTCAGTTGGACATAAAACCTGCTGGAATTCCTTTAGATATTGTTTGTGAAAGCTTGGAGGCTGCACGTAAAGGTCGTATTCAAATTCTTGATCACATGGAGAGAGAGATCAGTGCACCACGTACTCAGGATGATAGAAATTCTCCACGATTAGCTACCATGAAGTACAGCAATGATGCTCTTCGCCGGTTGCTTGGGCCTATGGGTGCTCTTAAAAGAAAAATTGAAGACGAAACAGGTACACGGATGTCTGTAAGTGATGGAACACTAACCATAGTTGCTAAGAATCAATCTGCAATGGACAAAGTACTCGAAAAGGTTGATTTTATACTTGGCCGTGAAATAGAAATCGGTGGGATCTATAAAGGGACTGTAGCTTCAATCAAAGAGTATGGTGCATTTGTGGAGTTTAATGGTGGGCAACAAGGCCTTTTACACATTTCTGAGATGTCACATGAACCGGTATCACGGATTTCAGATGTAGTATCTGTTGGCCAGCAGCTCTCTTTGATGTGCATAGGCCAGGATATCCGTGGTAACATTAAACTGTCCCTAAAAGCTGCATCACCTCGACCACAATCTCAGGCAAATAATCTGGTGGAACCTGTTCTATCTATGAAACAATCTCCTAGTGTTTGGGCAGCCGCTGGAGATGTGTCTACTTCCGAAGAAAAGAATAGCCCTTTGTCTGAGGAATTGCCAGTTAGTAAATATGAAGTTAATGATATAAAATCATCTAGTTCCTCTAGTTCACCAGTTCTAGTCAGAAGTGTTGAGGAGTGTGACGAGAAGGAAAGATCTACTGGTTTTGTTCAAAGTTCTAAAACTACTTCTGAACCCATTGGTGGTTTGAAACCAGAGCGCAAGACTGTTCGAACTACTCGTAAGACATCAAAATCTTTCTTAAAAAAGGGAGAAAAAGATGAGAAGGAACACAATATTTCGTCCCAAACCCACTCTAGCAATGATTTGAGTAGCAAGGAAGATTATGTAACAGCAAGAAGTCTTAAGCTAGGAACAAAGGTTACTGCCAAGGTTTATCAGGTCCGCACGGGTGGGCTGGTGCTTGATCTGGGTGGGGGACTTCGAGGAATGTATCGCTTTGAGGCCGATGGTAAAAAGGACTTTGATGTCAACGATGAGTTGCAAGTTGAGTGCGTAAGTTTCTCTAGCAAGGGCATTCCAGTGATGTCTTTGGTGGATGATGAATAG

Protein Analysis

970

Amino Acids

106.2

Weight (kDa)

6.0

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_PH PF01138 67 - 197 1.7e-26 3' exoribonuclease family, domain 1
RNase_PH_C PF03725 200 - 262 7.8e-11 3' exoribonuclease family, domain 2
PNPase PF03726 296 - 375 1.5e-16 Polyribonucleotide nucleotidyltransferase, RNA binding domain
RNase_PH PF01138 378 - 511 1.2e-28 3' exoribonuclease family, domain 1
S1 PF00575 681 - 751 1.1e-10 S1 RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2828
Acc16I TGCGCA 1 cut(s) 1538
Acc36I ACCTGC 2 cut(s) 1201, 1726
AccBSI CCGCTC 1 cut(s) 34
AccI GTMKAC 2 cut(s) 1056, 2357
AccII CGCG 4 cut(s) 11, 24, 365, 899
AccIII TCCGGA 1 cut(s) 787
AclI AACGTT 1 cut(s) 716
AclWI GGATC 7 cut(s) 63, 205, 527, 932, 1111, 1189, 2063
AcoI YGGCCR 6 cut(s) 3, 40, 97, 449, 2032, 2191
AcsI RAATTY 4 cut(s) 1424, 1725, 1782, 1837
AcuI CTGAAG 2 cut(s) 1437, 1473
AcyI GRCGYC 1 cut(s) 312
AdeI CACNNNGTG 1 cut(s) 1244
AfaI GTAC 8 cut(s) 703, 1148, 1215, 1222, 1822, 1865, 1939, 2007
AfeI AGCGCT 1 cut(s) 1289
AfiI CCNNNNNNNGG 7 cut(s) 38, 596, 658, 705, 947, 1651, 2543
AflII CTTAAG 2 cut(s) 673, 2705
AflIII ACRYGT 1 cut(s) 1543
AgeI ACCGGT 1 cut(s) 2158
AjnI CCWGG 2 cut(s) 1240, 2220
Alw21I GWGCWC 5 cut(s) 409, 436, 1240, 1816, 1909
Alw26I GTCTC 4 cut(s) 212, 1030, 1197, 1561
Alw44I GTGCAC 2 cut(s) 405, 1812
AlwI GGATC 7 cut(s) 63, 205, 527, 932, 1111, 1189, 2063
AlwNI CAGNNNCTG 1 cut(s) 2345
Ama87I CYCGRG 1 cut(s) 215
Aor13HI TCCGGA 1 cut(s) 787
Aor51HI AGCGCT 1 cut(s) 1289
ApaI GGGCCC 1 cut(s) 602
ApaLI GTGCAC 2 cut(s) 405, 1812
ApeKI GCWGC 9 cut(s) 93, 548, 566, 827, 1534, 1760, 2197, 2258, 2339
ApoI RAATTY 4 cut(s) 1424, 1725, 1782, 1837
AsiGI ACCGGT 1 cut(s) 2158
Asp700I GAANNNNTTC 2 cut(s) 359, 2304
AspLEI GCGC 6 cut(s) 26, 367, 901, 1290, 1539, 2562
AspS9I GGNCC 6 cut(s) 16, 598, 599, 1274, 1895, 2743
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 8 cut(s) 169, 320, 328, 508, 1256, 1684, 2256, 2442
AsuII TTCGAA 1 cut(s) 2572
AvaI CYCGRG 1 cut(s) 215
AvaII GGWCC 2 cut(s) 1274, 2743
BaeGI GKGCMC 3 cut(s) 409, 602, 1816
BalI TGGCCA 2 cut(s) 5, 2193
BanII GRGCYC 1 cut(s) 602
BauI CACGAG 3 cut(s) 276, 738, 1197
BbsI GAAGAC 2 cut(s) 1647, 1932
Bbv12I GWGCWC 5 cut(s) 409, 436, 1240, 1816, 1909
BbvI GCAGC 9 cut(s) 105, 535, 553, 814, 1521, 1747, 2209, 2245, 2351
BccI CCATC 8 cut(s) 515, 1109, 1288, 1463, 1510, 1679, 1952, 2804
BceAI ACGGC 4 cut(s) 262, 299, 516, 2019
BciT130I CCWGG 2 cut(s) 1242, 2222
BclI TGATCA 3 cut(s) 207, 1582, 1789
BcnI CCSGG 1 cut(s) 153
BcoDI GTCTC 4 cut(s) 212, 1030, 1197, 1561
BfaI CTAG 8 cut(s) 761, 2328, 2436, 2445, 2459, 2658, 2711, 2868
BfmI CTRYAG 5 cut(s) 91, 471, 918, 1303, 2070
BfoI RGCGCY 1 cut(s) 1291
BfrI CTTAAG 2 cut(s) 673, 2705
BfuAI ACCTGC 2 cut(s) 1201, 1726
BglI GCCNNNNNGGC 2 cut(s) 11, 105
BglII AGATCT 2 cut(s) 757, 2495
BlpI GCTNAGC 1 cut(s) 668
BmcAI AGTACT 1 cut(s) 2007
Bme1390I CCNGG 3 cut(s) 153, 1242, 2222
Bme18I GGWCC 2 cut(s) 1274, 2743
BmeT110I CYCGRG 1 cut(s) 215
BmgT120I GGNCC 6 cut(s) 16, 598, 599, 1274, 1895, 2743
BmiI GGNNCC 6 cut(s) 157, 250, 600, 601, 1275, 2301
BmrFI CCNGG 3 cut(s) 153, 1242, 2222
BmsI GCATC 5 cut(s) 861, 1237, 1510, 1864, 2270
BpiI GAAGAC 2 cut(s) 1647, 1932
BplI GAGNNNNNCTC 2 cut(s) 766, 798
BpmI CTGGAG 1 cut(s) 2367
Bpu1102I GCTNAGC 1 cut(s) 668
Bpu14I TTCGAA 1 cut(s) 2572
BpuEI CTTGAG 2 cut(s) 1054, 1665
BpuMI CCSGG 1 cut(s) 153
BsaAI YACGTR 2 cut(s) 1766, 1820
BsaHI GRCGYC 1 cut(s) 312
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 9 cut(s) 9, 37, 73, 303, 590, 972, 1560, 2230, 2730
BsaWI WCCGGW 3 cut(s) 221, 787, 2158
BsaXI ACNNNNNCTCC 2 cut(s) 388, 418
Bsc4I CCNNNNNNNGG 7 cut(s) 38, 596, 658, 705, 947, 1651, 2543
Bse118I RCCGGY 2 cut(s) 1884, 2158
Bse1I ACTGG 6 cut(s) 828, 1597, 2399, 2453, 2506, 2882
Bse3DI GCAATG 3 cut(s) 1876, 2001, 2668
BseAI TCCGGA 1 cut(s) 787
BseBI CCWGG 2 cut(s) 1242, 2222
BseDI CCNNGG 9 cut(s) 9, 37, 73, 303, 590, 972, 1560, 2230, 2730
BseGI GGATG 6 cut(s) 667, 784, 1525, 1834, 1950, 2905
BseLI CCNNNNNNNGG 7 cut(s) 38, 596, 658, 705, 947, 1651, 2543
BseMI GCAATG 3 cut(s) 1876, 2001, 2668
BseMII CTCAG 5 cut(s) 867, 1838, 2133, 2294, 2379
BseNI ACTGG 6 cut(s) 828, 1597, 2399, 2453, 2506, 2882
BseRI GAGGAG 5 cut(s) 26, 103, 155, 409, 2489
BseSI GKGCMC 3 cut(s) 409, 602, 1816
BseX3I CGGCCG 3 cut(s) 40, 97, 449
BseXI GCAGC 9 cut(s) 105, 535, 553, 814, 1521, 1747, 2209, 2245, 2351
BseYI CCCAGC 1 cut(s) 767
BsgI GTGCAG 1 cut(s) 1746
Bsh1236I CGCG 4 cut(s) 11, 24, 365, 899
Bsh1285I CGRYCG 3 cut(s) 43, 100, 452
BshTI ACCGGT 1 cut(s) 2158
BsiEI CGRYCG 3 cut(s) 43, 100, 452
BsiHKAI GWGCWC 5 cut(s) 409, 436, 1240, 1816, 1909
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 5 cut(s) 153, 222, 788, 1885, 2159
BslFI GGGAC 7 cut(s) 444, 722, 1667, 2080, 2233, 2629, 2793
BslI CCNNNNNNNGG 7 cut(s) 38, 596, 658, 705, 947, 1651, 2543
BsmAI GTCTC 4 cut(s) 212, 1030, 1197, 1561
BsmFI GGGAC 7 cut(s) 444, 722, 1667, 2080, 2233, 2629, 2793
BsmI GAATGC 2 cut(s) 1527, 2877
Bso31I GGTCTC 1 cut(s) 212
BsoBI CYCGRG 1 cut(s) 215
Bsp119I TTCGAA 1 cut(s) 2572
Bsp120I GGGCCC 1 cut(s) 598
Bsp1286I GDGCHC 6 cut(s) 409, 436, 602, 1240, 1816, 1909
Bsp13I TCCGGA 1 cut(s) 787
Bsp1407I TGTACA 1 cut(s) 701
Bsp1720I GCTNAGC 1 cut(s) 668
BspCNI CTCAG 5 cut(s) 868, 1837, 2134, 2293, 2380
BspEI TCCGGA 1 cut(s) 787
BspFNI CGCG 4 cut(s) 11, 24, 365, 899
BspHI TCATGA 1 cut(s) 390
BspLI GGNNCC 6 cut(s) 157, 250, 600, 601, 1275, 2301
BspMAI CTGCAG 2 cut(s) 95, 475
BspMI ACCTGC 2 cut(s) 1201, 1726
BspPI GGATC 7 cut(s) 63, 205, 527, 932, 1111, 1189, 2063
BspQI GCTCTTC 1 cut(s) 1884
BspT104I TTCGAA 1 cut(s) 2572
BspTI CTTAAG 2 cut(s) 673, 2705
BspTNI GGTCTC 1 cut(s) 212
BsrBI CCGCTC 1 cut(s) 34
BsrDI GCAATG 3 cut(s) 1876, 2001, 2668
BsrFI RCCGGY 2 cut(s) 1884, 2158
BsrGI TGTACA 1 cut(s) 701
BsrI ACTGG 6 cut(s) 828, 1597, 2399, 2453, 2506, 2882
BssAI RCCGGY 2 cut(s) 1884, 2158
BssECI CCNNGG 9 cut(s) 9, 37, 73, 303, 590, 972, 1560, 2230, 2730
BssNI GRCGYC 1 cut(s) 312
BssSI CACGAG 3 cut(s) 276, 738, 1197
BssT1I CCWWGG 4 cut(s) 303, 590, 972, 2730
Bst2BI CACGAG 3 cut(s) 276, 738, 1197
Bst2UI CCWGG 2 cut(s) 1242, 2222
Bst6I CTCTTC 2 cut(s) 1008, 1884
BstACI GRCGYC 1 cut(s) 312
BstAFI CTTAAG 2 cut(s) 673, 2705
BstAPI GCANNNNNTGC 1 cut(s) 90
BstAUI TGTACA 1 cut(s) 701
BstBAI YACGTR 2 cut(s) 1766, 1820
BstBI TTCGAA 1 cut(s) 2572
BstC8I GCNNGC 8 cut(s) 235, 475, 598, 735, 838, 842, 1525, 2195
BstDEI CTNAG 8 cut(s) 668, 688, 876, 1824, 1980, 2142, 2280, 2388
BstDSI CCRYGG 3 cut(s) 9, 1560, 2230
BstENI CCTNNNNNAGG 1 cut(s) 703
BstF5I GGATG 6 cut(s) 667, 784, 1525, 1834, 1950, 2905
BstFNI CGCG 4 cut(s) 11, 24, 365, 899
BstH2I RGCGCY 1 cut(s) 1291
BstHHI GCGC 6 cut(s) 26, 367, 901, 1290, 1539, 2562
BstMAI GTCTC 4 cut(s) 212, 1030, 1197, 1561
BstMCI CGRYCG 3 cut(s) 43, 100, 452
BstNI CCWGG 2 cut(s) 1242, 2222
BstNSI RCATGY 1 cut(s) 1547
BstSCI CCNGG 3 cut(s) 151, 1240, 2220
BstSFI CTRYAG 5 cut(s) 91, 471, 918, 1303, 2070
BstSLI GKGCMC 3 cut(s) 409, 602, 1816
BstUI CGCG 4 cut(s) 11, 24, 365, 899
BstV1I GCAGC 9 cut(s) 105, 535, 553, 814, 1521, 1747, 2209, 2245, 2351
BstV2I GAAGAC 2 cut(s) 1647, 1932
BstX2I RGATCY 4 cut(s) 532, 757, 2055, 2495
BstYI RGATCY 4 cut(s) 532, 757, 2055, 2495
BstZI CGGCCG 3 cut(s) 40, 97, 449
BtgI CCRYGG 3 cut(s) 9, 1560, 2230
BtsCI GGATG 6 cut(s) 667, 784, 1525, 1834, 1950, 2905
BtsIMutI CAGTG 6 cut(s) 866, 933, 1068, 1434, 1816, 2889
BveI ACCTGC 2 cut(s) 1201, 1726
Cac8I GCNNGC 8 cut(s) 235, 475, 598, 735, 838, 842, 1525, 2195
CaiI CAGNNNCTG 1 cut(s) 2345
CciI TCATGA 1 cut(s) 390
CciNI GCGGCCGC 1 cut(s) 97
CfoI GCGC 6 cut(s) 26, 367, 901, 1290, 1539, 2562
Cfr10I RCCGGY 2 cut(s) 1884, 2158
Cfr13I GGNCC 6 cut(s) 16, 598, 599, 1274, 1895, 2743
Cfr42I CCGCGG 1 cut(s) 12
CseI GACGC 1 cut(s) 320
CsiI ACCWGGT 1 cut(s) 1240
Csp6I GTAC 8 cut(s) 702, 1147, 1214, 1221, 1821, 1864, 1938, 2006
CspAI ACCGGT 1 cut(s) 2158
CviQI GTAC 8 cut(s) 702, 1147, 1214, 1221, 1821, 1864, 1938, 2006
DdeI CTNAG 8 cut(s) 668, 688, 876, 1824, 1980, 2142, 2280, 2388
DraIII CACNNNGTG 1 cut(s) 1244
DrdI GACNNNNNNGTC 1 cut(s) 2828
DseDI GACNNNNNNGTC 1 cut(s) 2828
EaeI YGGCCR 6 cut(s) 3, 40, 97, 449, 2032, 2191
EagI CGGCCG 3 cut(s) 40, 97, 449
Eam1104I CTCTTC 2 cut(s) 1008, 1884
EarI CTCTTC 2 cut(s) 1008, 1884
EciI GGCGGA 2 cut(s) 289, 1400
EclXI CGGCCG 3 cut(s) 40, 97, 449
Eco130I CCWWGG 4 cut(s) 303, 590, 972, 2730
Eco147I AGGCCT 2 cut(s) 1141, 2127
Eco24I GRGCYC 1 cut(s) 602
Eco31I GGTCTC 1 cut(s) 212
Eco32I GATATC 1 cut(s) 2227
Eco47I GGWCC 2 cut(s) 1274, 2743
Eco47III AGCGCT 1 cut(s) 1289
Eco52I CGGCCG 3 cut(s) 40, 97, 449
Eco57I CTGAAG 2 cut(s) 1437, 1473
Eco88I CYCGRG 1 cut(s) 215
EcoNI CCTNNNNNAGG 1 cut(s) 703
EcoO109I RGGNCCY 2 cut(s) 599, 1274
EcoRI GAATTC 1 cut(s) 1725
EcoRII CCWGG 2 cut(s) 1240, 2220
EcoRV GATATC 1 cut(s) 2227
EcoT14I CCWWGG 4 cut(s) 303, 590, 972, 2730
EcoT38I GRGCYC 1 cut(s) 602
ErhI CCWWGG 4 cut(s) 303, 590, 972, 2730
FaqI GGGAC 7 cut(s) 444, 722, 1667, 2080, 2233, 2629, 2793
FauI CCCGC 2 cut(s) 39, 589
FbaI TGATCA 3 cut(s) 207, 1582, 1789
FblI GTMKAC 2 cut(s) 1056, 2357
FokI GGATG 5 cut(s) 674, 771, 1532, 1841, 1957
FriOI GRGCYC 1 cut(s) 602
FspBI CTAG 8 cut(s) 761, 2328, 2436, 2445, 2459, 2658, 2711, 2868
FspI TGCGCA 1 cut(s) 1538
GlaI GCGC 6 cut(s) 25, 366, 900, 1289, 1538, 2561
GsaI CCCAGC 1 cut(s) 771
GsuI CTGGAG 1 cut(s) 2367
HaeII RGCGCY 1 cut(s) 1291
HapII CCGG 5 cut(s) 153, 222, 788, 1885, 2159
HgaI GACGC 1 cut(s) 320
HhaI GCGC 6 cut(s) 26, 367, 901, 1290, 1539, 2562
Hin1I GRCGYC 1 cut(s) 312
Hin6I GCGC 6 cut(s) 24, 365, 899, 1288, 1537, 2560
HinP1I GCGC 6 cut(s) 24, 365, 899, 1288, 1537, 2560
HincII GTYRAC 2 cut(s) 649, 2833
HindII GTYRAC 2 cut(s) 649, 2833
HindIII AAGCTT 2 cut(s) 1030, 1750
HinfI GANTC 9 cut(s) 137, 454, 613, 1038, 1125, 1154, 1262, 1689, 1984
HpaI GTTAAC 1 cut(s) 649
HpaII CCGG 5 cut(s) 153, 222, 788, 1885, 2159
HphI GGTGA 8 cut(s) 169, 320, 328, 508, 1256, 1684, 2256, 2442
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 5 cut(s) 184, 844, 1094, 2482, 2620
HpyCH4IV ACGT 5 cut(s) 560, 716, 961, 1765, 1819
HpyF3I CTNAG 8 cut(s) 668, 688, 876, 1824, 1980, 2142, 2280, 2388
HpySE526I ACGT 5 cut(s) 560, 716, 961, 1765, 1819
Hsp92I GRCGYC 1 cut(s) 312
HspAI GCGC 6 cut(s) 24, 365, 899, 1288, 1537, 2560
Kpn2I TCCGGA 1 cut(s) 787
Ksp22I TGATCA 3 cut(s) 207, 1582, 1789
KspAI GTTAAC 1 cut(s) 649
KspI CCGCGG 1 cut(s) 12
LguI GCTCTTC 1 cut(s) 1884
LmnI GCTCC 6 cut(s) 39, 168, 254, 302, 919, 1235
Lsp1109I GCAGC 9 cut(s) 105, 535, 553, 814, 1521, 1747, 2209, 2245, 2351
LweI GCATC 5 cut(s) 861, 1237, 1510, 1864, 2270
MabI ACCWGGT 1 cut(s) 1240
MaeI CTAG 8 cut(s) 761, 2328, 2436, 2445, 2459, 2658, 2711, 2868
MaeII ACGT 5 cut(s) 560, 716, 961, 1765, 1819
MbiI CCGCTC 1 cut(s) 34
MflI RGATCY 4 cut(s) 532, 757, 2055, 2495
MhlI GDGCHC 6 cut(s) 409, 436, 602, 1240, 1816, 1909
MlsI TGGCCA 2 cut(s) 5, 2193
MluNI TGGCCA 2 cut(s) 5, 2193
MlyI GAGTC 3 cut(s) 1032, 1119, 1698
MmeI TCCRAC 2 cut(s) 1239, 1686
Mox20I TGGCCA 2 cut(s) 5, 2193
MroI TCCGGA 1 cut(s) 787
MroXI GAANNNNTTC 2 cut(s) 359, 2304
MscI TGGCCA 2 cut(s) 5, 2193
MslI CAYNNNNRTG 3 cut(s) 389, 608, 2882
Msp20I TGGCCA 2 cut(s) 5, 2193
MspA1I CMGCKG 4 cut(s) 11, 96, 1534, 2345
MspCI CTTAAG 2 cut(s) 673, 2705
MspI CCGG 5 cut(s) 153, 222, 788, 1885, 2159
MspR9I CCNGG 3 cut(s) 153, 1242, 2222
Mva1269I GAATGC 2 cut(s) 1527, 2877
MvaI CCWGG 2 cut(s) 1242, 2222
MvnI CGCG 4 cut(s) 11, 24, 365, 899
NciI CCSGG 1 cut(s) 153
NlaIV GGNNCC 6 cut(s) 157, 250, 600, 601, 1275, 2301
NmeAIII GCCGAG 2 cut(s) 18, 54
NmuCI GTSAC 6 cut(s) 308, 496, 864, 1690, 2148, 2480
NotI GCGGCCGC 1 cut(s) 97
NsbI TGCGCA 1 cut(s) 1538
NspI RCATGY 1 cut(s) 1547
NspV TTCGAA 1 cut(s) 2572
PaeR7I CTCGAG 1 cut(s) 215
PagI TCATGA 1 cut(s) 390
PceI AGGCCT 2 cut(s) 1141, 2127
PciI ACATGT 1 cut(s) 1543
PciSI GCTCTTC 1 cut(s) 1884
PcsI WCGNNNNNNNCGW 4 cut(s) 252, 454, 1771, 2578
PctI GAATGC 2 cut(s) 1527, 2877
PdmI GAANNNNTTC 2 cut(s) 359, 2304
PfeI GAWTC 6 cut(s) 137, 454, 613, 1154, 1262, 1984
PinAI ACCGGT 1 cut(s) 2158
PleI GAGTC 3 cut(s) 1032, 1119, 1697
PpsI GAGTC 3 cut(s) 1032, 1119, 1697
Ppu21I YACGTR 2 cut(s) 1766, 1820
PpuMI RGGWCCY 1 cut(s) 1274
PscI ACATGT 1 cut(s) 1543
Psp1406I AACGTT 1 cut(s) 716
Psp5II RGGWCCY 1 cut(s) 1274
Psp6I CCWGG 2 cut(s) 1240, 2220
PspFI CCCAGC 1 cut(s) 767
PspGI CCWGG 2 cut(s) 1240, 2220
PspN4I GGNNCC 6 cut(s) 157, 250, 600, 601, 1275, 2301
PspOMI GGGCCC 1 cut(s) 598
PspPI GGNCC 6 cut(s) 16, 598, 599, 1274, 1895, 2743
PspPPI RGGWCCY 1 cut(s) 1274
PsrI GAACNNNNNNTAC 2 cut(s) 2707, 2739
PstI CTGCAG 2 cut(s) 95, 475
PstNI CAGNNNCTG 1 cut(s) 2345
PsuI RGATCY 4 cut(s) 532, 757, 2055, 2495
RsaI GTAC 8 cut(s) 703, 1148, 1215, 1222, 1822, 1865, 1939, 2007
RsaNI GTAC 8 cut(s) 702, 1147, 1214, 1221, 1821, 1864, 1938, 2006
RseI CAYNNNNRTG 3 cut(s) 389, 608, 2882
SacII CCGCGG 1 cut(s) 12
SapI GCTCTTC 1 cut(s) 1884
Sau96I GGNCC 6 cut(s) 16, 598, 599, 1274, 1895, 2743
SbfI CCTGCAGG 1 cut(s) 475
ScaI AGTACT 1 cut(s) 2007
SchI GAGTC 3 cut(s) 1032, 1119, 1698
ScrFI CCNGG 3 cut(s) 153, 1242, 2222
SdaI CCTGCAGG 1 cut(s) 475
SduI GDGCHC 6 cut(s) 409, 436, 602, 1240, 1816, 1909
SexAI ACCWGGT 1 cut(s) 1240
SfaNI GCATC 5 cut(s) 861, 1237, 1510, 1864, 2270
SfcI CTRYAG 5 cut(s) 91, 471, 918, 1303, 2070
Sfr274I CTCGAG 1 cut(s) 215
Sfr303I CCGCGG 1 cut(s) 12
SfuI TTCGAA 1 cut(s) 2572
SgrBI CCGCGG 1 cut(s) 12
SinI GGWCC 2 cut(s) 1274, 2743
SlaI CTCGAG 1 cut(s) 215
SmiMI CAYNNNNRTG 3 cut(s) 389, 608, 2882
SmlI CTYRAG 5 cut(s) 215, 673, 1033, 1680, 2705
SmoI CTYRAG 5 cut(s) 215, 673, 1033, 1680, 2705
Sse8387I CCTGCAGG 1 cut(s) 475
SseBI AGGCCT 2 cut(s) 1141, 2127
SspI AATATT 2 cut(s) 982, 2638
SspMI CTAG 8 cut(s) 761, 2328, 2436, 2445, 2459, 2658, 2711, 2868
StuI AGGCCT 2 cut(s) 1141, 2127
StyD4I CCNGG 3 cut(s) 151, 1240, 2220
StyI CCWWGG 4 cut(s) 303, 590, 972, 2730
TaiI ACGT 5 cut(s) 563, 719, 964, 1768, 1822
TaqI TCGA 9 cut(s) 148, 168, 189, 216, 1128, 2010, 2269, 2572, 2785
TatI WGTACW 5 cut(s) 701, 1146, 1220, 1863, 2005
TauI GCSGC 6 cut(s) 14, 17, 45, 99, 102, 2345
TfiI GAWTC 6 cut(s) 137, 454, 613, 1154, 1262, 1984
TscAI CASTG 6 cut(s) 873, 940, 1068, 1441, 1816, 2889
TseFI GTSAC 6 cut(s) 308, 496, 864, 1690, 2148, 2480
TseI GCWGC 9 cut(s) 93, 548, 566, 827, 1534, 1760, 2197, 2258, 2339
Tsp45I GTSAC 6 cut(s) 308, 496, 864, 1690, 2148, 2480
TspDTI ATGAA 9 cut(s) 379, 1038, 1146, 1282, 1437, 1874, 2169, 2330, 2427
TspGWI ACGGA 7 cut(s) 241, 1070, 1430, 1549, 1957, 2182, 2219
TspRI CASTG 6 cut(s) 873, 940, 1068, 1441, 1816, 2889
Vha464I CTTAAG 2 cut(s) 673, 2705
VneI GTGCAC 2 cut(s) 405, 1812
VpaK11BI GGWCC 2 cut(s) 1274, 2743
XagI CCTNNNNNAGG 1 cut(s) 703
XapI RAATTY 4 cut(s) 1424, 1725, 1782, 1837
XbaI TCTAGA 1 cut(s) 760
XceI RCATGY 1 cut(s) 1547
XcmI CCANNNNNNNNNTGG 1 cut(s) 2228
XhoI CTCGAG 1 cut(s) 215
XmiI GTMKAC 2 cut(s) 1056, 2357
XmnI GAANNNNTTC 2 cut(s) 359, 2304
XspI CTAG 8 cut(s) 761, 2328, 2436, 2445, 2459, 2658, 2711, 2868
ZrmI AGTACT 1 cut(s) 2007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.