Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004785

Physical Location & Seq

Genomic Coordinates Reverse (-)

2256412 .. 2262619

Gene Structure 6208 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1031200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2835 bp

ATGGCGTCCATGGCCAACCGAGCCAATCCGCTGCTCAACAACCTCCCTCACTTCCTCACATGGCGAGCCTTAGGGTTCCGCACCATCTGCAGCGGCCGCATGGGCTTCTCGTCTCAGTCCGAGCGGCAGCCTGATCCCGAAACCCCCGTCGCCGGCACCAAGGTGCTCGAGACTTTCAGAGAAGAATTTGAAGTCGGAGATCGATTAATTACGCTCGAAACGGGAAAGATCGCTCGCTTCGCTAACGGTGCCGTTGTTTTGGGCATGGAGGAGACCAAAGTATTATCCACCGTGGCCGCCGCCAAAGGCGACGCCGTTCGCGATTTTCTGCCCCTAACTGTTGATTATCAAGAGAAGCAATTTGCCCAAGGAGTAATTCCGAACACATTCATGCGAAGGGAGGGTGCGCCGAAGGAGCGTGAACTCTTATGTGGTCGTATCATTGATCGACCAATACGTCCGCTATTTCCTGCTGGGTTTTTCCATGAAGTTCAGGTAACAGCAAGTGTTCTTTCCTCTGATGGAAAGCAAGATCCAGATGTACTGGTAGCTAATGCTACATCTGCTGCGCTTATGTTATCAGATATTCCTTGGGGTGGCCCCATTGGCATGATTCGTATAGGCAGGATTGCTGGGAAGTTTATTGTTAATCCAACTATGGATGAGCTTAGCTTGAGTGATCTCAACTTAGTGTATGCCTGTACGAGAGACAAAACGTTAATGATTGATGTGCAGGCTCGTGAGATCTCAGAAAAAGATCTAGAAGCAGGGTTAAGATTAGCTCATCCCGAGGCAGTTAAATATATTGAACCTCAAATCAAACTGGCTGCTAAAGCTGGTAAGCATAAGAAGGAATATAAATTGTCAATGATGCCAGATAGAACATTGGAGAAAGTCAGTCAATTGGCAGAAGCTCCAATAGAAGCCGTTTTCACTGATCCATCATATGGAAAGTTTGAACGTGGAGAGGCCTTAGAGAATATTACACAAGACGTGAGAAAAGTACTTGAAGAAGAGTGTGATGAAGAAAGCTTGAAACTTCTACCAAAGGCAGTAGATACTGTGAGGAAAAAGGTTGTTCGTAAAAGAATTATTGCGGAAGGAGTCAGACTTGATGGGAGGCGTCTTGATGAAGTTAGGCCTTTGTATTGTGAAGCTGGTCATTTGCCTATGCTGCATGGATCATCACTTTTTTCTCGTGGAGACACACAGGTTCTTTGTACTGTAACACTTGGGGCGCCTGGTGATGCTCAACGATTGGATTCTCTGGTTGGGCCTCCAACAAAACGTTTCATGCTTCACTACAGCTTTCCCCCTTTCTGTATTAATGAAGTTGGTAAACGAGGCGGCTTGAATAGGCGTGAAGTTGGTCATGGGACTCTGGCGGAAAAAGCTCTTCTTGCTGTATTACCTCCAGAAGATGATTTTCCATATACTGTTCGTATCAATTCTGAAGTAATGGCCTCTGACGGTTCAACATCAATGGCAACTGTCTGTGGAGGCAGTATGGCTCTGATGGATGCTGGCATTCCATTGCGTGAACATGTAGCTGGTGTCTCGGTGGGTCTTGTTAGTGAAGTTGATCAATCAACTGGCTCAATAAAGGAGTACCGTATACTGACTGATATTCTGGGCCTGGAAGATCATTTGGGGGACATGGACTTTAAAATTGCTGGCACACTAAATGGAGTTACAGCTATTCAGTTGGATATAAAACCCGCTGGAATTCCTTTAGATATTGTCTGTGAAAGCTTAGAACCTGCACGCAAAGGTCGCATTCAAATTCTTGACCACATGGAGAGAGAGATCAGTACACCACGCACTCAGGATGATAGAAATTCTCCACGATTAGTTACCTTGAAGTACAGTAATGATGCACTTCGTCGCTTGCTTGGGCCTCTCGGTGCTCTAAAAAGAAAAATTGAAGATGAAACTGGTGCACGGATGTCTGTAAGTGATGGAACACTGACCATAGTTGCTAAGAATCAATCTGTAATGGAGAAAGTACTAGAAAAGGTTGATTTTATACTTGGGCGTGAGATCGAAATTGGTGGGATCTATAAGGGAAGGGTAACTTCAGTCAAGGAATATGGTGCATTTGTGGAGTTTAATGGTGGTCAGCAAGGCCTTCTGCACATTTCTGAGTTGTCGCAAACACCAGTATCTCGGGTTTCAGATGTGGTAGCTGTTGGAGAACAGCTTTCCTTGATGTGCATAGGTCAGGATGTTCGCGGTAACATTAAACTATCCCTTAAAGCTACTTTACCTCGATCTGGATCCAAGACAAATAATGCGGTTGAGGAATCCGTTTCATCAACCCAAGAACCTAGTATTTGGGCAGCAGCTGGAGACCTTTTGTCTAATGGACAAGAAAAGCAGAGCTCGTCATCTGAGGAATTGCCAGCAAACAATTGTGAAGTTGGCGGGATAAATTCATCTACTTCCTCTACTCCGATTCTAATTCGAAGTGCTGCAGAGTGTGACGAGGTGGAAAAAGCTGCTGGTTTGGTTCAAAACTCTGATACTACTAAGTCAAAAACTAGTAATGTGAAGACATCAAAGTCGTCCTCACAGAAGGGAGGAGATAAGGAAGCTGAAGTTGGAGGCTCTGTAACCGAAAAAAGTCTGAAGATAGGAACAAAAGTTACTGCCAAGGTTTATCAGATCCGCACAGGAGGGTTGGTGCTTGACCTCGGTGGGGGACTTCGTGGAATGTATCGGTTTGAGACAAATGGTAGGACGGACTTTGAAGTCAATGACGAGCTGCGAGTTGAGTGTGTTAGTTTCTCTGGCAAGGGGATTCCAGTAATGTCTCTGGTGGATGATGAAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000175 GO:0000957 GO:0000959 GO:0000963 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0004654 GO:0006139 GO:0006396 GO:0006401 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008408 GO:0009056 GO:0009057 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019439 GO:0034641 GO:0034655 GO:0043170 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0046483 GO:0046700 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140053 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko03018 map00230 map00240 map03018

Pfam Domains

Protein Families

KH_1 PNPase RNase_PH RNase_PH_C S1

Protein Analysis

945

Amino Acids

102.54

Weight (kDa)

5.61

Isoelectric Point (pI)

35.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNase_PH	PF01138	68 - 197	5.8e-25	3' exoribonuclease family, domain 1
RNase_PH_C	PF03725	200 - 262	4.3e-11	3' exoribonuclease family, domain 2
PNPase	PF03726	296 - 375	1.2e-16	Polyribonucleotide nucleotidyltransferase, RNA binding domain
RNase_PH	PF01138	378 - 511	4.2e-28	3' exoribonuclease family, domain 1
S1	PF00575	681 - 752	9.8e-12	S1 RNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004785)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14580
fragaria_vesca	FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050
malus_domestica	MD17G1031200.v1.1
prunus_persica	Prupe.3G288400_v2.0.a1
pyrus_communis	pycom17g02860
rosa_chinensis	RchiOBHm_Chr2g0172601
rosa_laevigata	RLG00000016016 RLG00000022119
rosa_multiflora	Rmu_sc0000912.1_g000001
rosa_roxburghii	Rroxscaffold_2G00079710
rosa_rugosa	Rorug01G0489200 Rorug02G0560200
rosa_samantha	Rh2AG636600 Rh2BG650000 Rh2CG616700 Rh2DG044700 Rh2DG664200
rosa_wichuraiana	Rw2G003770 Rw2G052650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	456, 2560, 2750
Acc36I	ACCTGC	1 cut(s)	1768
AccB1I	GGYRCC	3 cut(s)	155, 248, 1237
AccB7I	CCANNNNNTGG	1 cut(s)	947
AccBSI	CCGCTC	1 cut(s)	124
AccI	GTMKAC	1 cut(s)	1615
AccII	CGCG	2 cut(s)	321, 2232
AclI	AACGTT	2 cut(s)	716, 1288
AclWI	GGATC	8 cut(s)	128, 527, 932, 1189, 2063, 2271, 2284, 2659
AcoI	YGGCCR	3 cut(s)	12, 94, 294
AcsI	RAATTY	5 cut(s)	185, 1725, 1782, 1837, 2431
AcuI	CTGAAG	4 cut(s)	1473, 2061, 2616, 2648
AcyI	GRCGYC	4 cut(s)	5, 312, 1123, 1238
AfaI	GTAC	8 cut(s)	543, 703, 1005, 1222, 1610, 1813, 1865, 2007
AfiI	CCNNNNNNNGG	5 cut(s)	152, 596, 947, 2273, 2698
AflIII	ACRYGT	1 cut(s)	1543
AhdI	GACNNNNNGTC	1 cut(s)	2356
AhlI	ACTAGT	1 cut(s)	2540
AjiI	CACGTC	1 cut(s)	994
AjnI	CCWGG	2 cut(s)	1240, 1635
AjuI	GAANNNNNNNTTGG	4 cut(s)	360, 392, 2583, 2615
AleI	CACNNNNGTG	1 cut(s)	161
Alw21I	GWGCWC	4 cut(s)	168, 1909, 1942, 2384
Alw26I	GTCTC	9 cut(s)	117, 164, 266, 702, 1197, 1561, 2343, 2720, 2817
Alw44I	GTGCAC	1 cut(s)	1938
AlwI	GGATC	8 cut(s)	128, 527, 932, 1189, 2063, 2271, 2284, 2659
AlwNI	CAGNNNCTG	1 cut(s)	2345
Ama87I	CYCGRG	3 cut(s)	167, 788, 2166
ApaLI	GTGCAC	1 cut(s)	1938
ApoI	RAATTY	5 cut(s)	185, 1725, 1782, 1837, 2431
ArsI	GACNNNNNNTTYG	2 cut(s)	132, 164
AseI	ATTAAT	2 cut(s)	206, 1326
Asp700I	GAANNNNTTC	1 cut(s)	386
AspLEI	GCGC	3 cut(s)	409, 571, 1240
AspS9I	GGNCC	4 cut(s)	599, 1274, 1633, 1895
AsuHPI	GGTGA	1 cut(s)	1256
AsuII	TTCGAA	1 cut(s)	2464
AvaI	CYCGRG	3 cut(s)	167, 788, 2166
AxyI	CCTNAGG	1 cut(s)	70
BaeGI	GKGCMC	1 cut(s)	1942
BaeI	ACNNNNGTAYC	2 cut(s)	2145, 2178
BalI	TGGCCA	1 cut(s)	14
BamHI	GGATCC	1 cut(s)	2276
BanI	GGYRCC	3 cut(s)	155, 248, 1237
BanII	GRGCYC	1 cut(s)	2384
BauI	CACGAG	2 cut(s)	738, 1197
BbsI	GAAGAC	1 cut(s)	2558
Bbv12I	GWGCWC	4 cut(s)	168, 1909, 1942, 2384
BccI	CCATC	6 cut(s)	92, 515, 949, 1109, 1510, 1952
BceAI	ACGGC	3 cut(s)	236, 299, 911
BciT130I	CCWGG	2 cut(s)	1242, 1637
BclI	TGATCA	1 cut(s)	1582
BcoDI	GTCTC	9 cut(s)	117, 164, 266, 702, 1197, 1561, 2343, 2720, 2817
BcuI	ACTAGT	1 cut(s)	2540
BfaI	CTAG	4 cut(s)	761, 2009, 2328, 2541
BfmI	CTRYAG	3 cut(s)	88, 1303, 2472
BfoI	RGCGCY	1 cut(s)	1241
BfuAI	ACCTGC	1 cut(s)	1768
BglI	GCCNNNNNGGC	2 cut(s)	102, 606
BglII	AGATCT	2 cut(s)	744, 757
BlpI	GCTNAGC	1 cut(s)	668
BmcAI	AGTACT	2 cut(s)	1005, 2007
Bme1390I	CCNGG	2 cut(s)	1242, 1637
BmeRI	GACNNNNNGTC	1 cut(s)	2356
BmeT110I	CYCGRG	3 cut(s)	167, 788, 2166
BmgBI	CACGTC	1 cut(s)	994
BmgT120I	GGNCC	4 cut(s)	599, 1274, 1633, 1895
BmiI	GGNNCC	6 cut(s)	77, 157, 250, 601, 1239, 2278
BmrFI	CCNGG	2 cut(s)	1242, 1637
BmsI	GCATC	4 cut(s)	861, 1237, 1510, 1864
BpiI	GAAGAC	1 cut(s)	2558
BpmI	CTGGAG	2 cut(s)	1398, 2367
Bpu1102I	GCTNAGC	1 cut(s)	668
Bpu14I	TTCGAA	1 cut(s)	2464
BpuEI	CTTGAG	1 cut(s)	694
Bsa29I	ATCGAT	1 cut(s)	202
BsaBI	GATNNNNATC	1 cut(s)	2275
BsaHI	GRCGYC	4 cut(s)	5, 312, 1123, 1238
BsaI	GGTCTC	2 cut(s)	266, 2343
BsaJI	CCNNGG	8 cut(s)	9, 159, 291, 367, 590, 789, 2652, 2692
BsaXI	ACNNNNNCTCC	4 cut(s)	2595, 2625, 2667, 2697
Bsc4I	CCNNNNNNNGG	5 cut(s)	152, 596, 947, 2273, 2698
Bse118I	RCCGGY	1 cut(s)	152
Bse1I	ACTGG	6 cut(s)	549, 828, 1597, 1939, 2159, 2804
Bse21I	CCTNAGG	1 cut(s)	70
Bse3DI	GCAATG	1 cut(s)	1532
Bse8I	GATNNNNATC	1 cut(s)	2275
BseBI	CCWGG	2 cut(s)	1242, 1637
BseCI	ATCGAT	1 cut(s)	202
BseDI	CCNNGG	8 cut(s)	9, 159, 291, 367, 590, 789, 2652, 2692
BseGI	GGATG	7 cut(s)	667, 784, 1525, 1834, 1950, 2230, 2827
BseJI	GATNNNNATC	1 cut(s)	2275
BseLI	CCNNNNNNNGG	5 cut(s)	152, 596, 947, 2273, 2698
BseMI	GCAATG	1 cut(s)	1532
BseMII	CTCAG	5 cut(s)	128, 762, 1838, 2133, 2382
BseNI	ACTGG	6 cut(s)	549, 828, 1597, 1939, 2159, 2804
BseRI	GAGGAG	2 cut(s)	284, 2595
BseSI	GKGCMC	1 cut(s)	1942
BseX3I	CGGCCG	1 cut(s)	94
BseYI	CCCAGC	2 cut(s)	473, 632
BsgI	GTGCAG	3 cut(s)	752, 1746, 2117
Bsh1236I	CGCG	2 cut(s)	321, 2232
Bsh1285I	CGRYCG	1 cut(s)	97
BshNI	GGYRCC	3 cut(s)	155, 248, 1237
BshVI	ATCGAT	1 cut(s)	202
BsiEI	CGRYCG	1 cut(s)	97
BsiHKAI	GWGCWC	4 cut(s)	168, 1909, 1942, 2384
BsiHKCI	CYCGRG	3 cut(s)	167, 788, 2166
BsiSI	CCGG	1 cut(s)	153
BslFI	GGGAC	3 cut(s)	1390, 1667, 2715
BslI	CCNNNNNNNGG	5 cut(s)	152, 596, 947, 2273, 2698
BsmAI	GTCTC	9 cut(s)	117, 164, 266, 702, 1197, 1561, 2343, 2720, 2817
BsmBI	CGTCTC	1 cut(s)	117
BsmFI	GGGAC	3 cut(s)	1390, 1667, 2715
BsmI	GAATGC	2 cut(s)	1527, 1776
Bso31I	GGTCTC	2 cut(s)	266, 2343
BsoBI	CYCGRG	3 cut(s)	167, 788, 2166
Bsp119I	TTCGAA	1 cut(s)	2464
Bsp1286I	GDGCHC	4 cut(s)	168, 1909, 1942, 2384
Bsp1720I	GCTNAGC	1 cut(s)	668
Bsp19I	CCATGG	1 cut(s)	9
Bsp68I	TCGCGA	1 cut(s)	321
BspCNI	CTCAG	5 cut(s)	127, 761, 1837, 2134, 2383
BspDI	ATCGAT	1 cut(s)	202
BspFNI	CGCG	2 cut(s)	321, 2232
BspLI	GGNNCC	6 cut(s)	77, 157, 250, 601, 1239, 2278
BspMAI	CTGCAG	2 cut(s)	92, 2476
BspMI	ACCTGC	1 cut(s)	1768
BspPI	GGATC	8 cut(s)	128, 527, 932, 1189, 2063, 2271, 2284, 2659
BspQI	GCTCTTC	1 cut(s)	1401
BspT104I	TTCGAA	1 cut(s)	2464
BspT107I	GGYRCC	3 cut(s)	155, 248, 1237
BspTNI	GGTCTC	2 cut(s)	266, 2343
BsrBI	CCGCTC	1 cut(s)	124
BsrDI	GCAATG	1 cut(s)	1532
BsrFI	RCCGGY	1 cut(s)	152
BsrI	ACTGG	6 cut(s)	549, 828, 1597, 1939, 2159, 2804
BssAI	RCCGGY	1 cut(s)	152
BssECI	CCNNGG	8 cut(s)	9, 159, 291, 367, 590, 789, 2652, 2692
BssNAI	GTATAC	1 cut(s)	1616
BssNI	GRCGYC	4 cut(s)	5, 312, 1123, 1238
BssSI	CACGAG	2 cut(s)	738, 1197
BssT1I	CCWWGG	5 cut(s)	9, 159, 367, 590, 2652
Bst1107I	GTATAC	1 cut(s)	1616
Bst2BI	CACGAG	2 cut(s)	738, 1197
Bst2UI	CCWGG	2 cut(s)	1242, 1637
Bst6I	CTCTTC	3 cut(s)	1008, 1401, 2823
BstACI	GRCGYC	4 cut(s)	5, 312, 1123, 1238
BstAPI	GCANNNNNTGC	1 cut(s)	87
BstBI	TTCGAA	1 cut(s)	2464
BstC8I	GCNNGC	9 cut(s)	66, 154, 235, 735, 1525, 1675, 1765, 1889, 2403
BstDSI	CCRYGG	2 cut(s)	9, 291
BstF5I	GGATG	7 cut(s)	667, 784, 1525, 1834, 1950, 2230, 2827
BstFNI	CGCG	2 cut(s)	321, 2232
BstH2I	RGCGCY	1 cut(s)	1241
BstHHI	GCGC	3 cut(s)	409, 571, 1240
BstMAI	GTCTC	9 cut(s)	117, 164, 266, 702, 1197, 1561, 2343, 2720, 2817
BstMCI	CGRYCG	1 cut(s)	97
BstNI	CCWGG	2 cut(s)	1242, 1637
BstNSI	RCATGY	1 cut(s)	1547
BstSCI	CCNGG	2 cut(s)	1240, 1635
BstSFI	CTRYAG	3 cut(s)	88, 1303, 2472
BstSLI	GKGCMC	1 cut(s)	1942
BstUI	CGCG	2 cut(s)	321, 2232
BstV2I	GAAGAC	1 cut(s)	2558
BstX2I	RGATCY	6 cut(s)	532, 744, 757, 2055, 2276, 2664
BstYI	RGATCY	6 cut(s)	532, 744, 757, 2055, 2276, 2664
BstZ17I	GTATAC	1 cut(s)	1616
BstZI	CGGCCG	1 cut(s)	94
Bsu15I	ATCGAT	1 cut(s)	202
Bsu36I	CCTNAGG	1 cut(s)	70
BsuTUI	ATCGAT	1 cut(s)	202
BtgI	CCRYGG	2 cut(s)	9, 291
BtrI	CACGTC	1 cut(s)	994
BtsCI	GGATG	7 cut(s)	667, 784, 1525, 1834, 1950, 2230, 2827
BtsIMutI	CAGTG	2 cut(s)	933, 1964
BtuMI	TCGCGA	1 cut(s)	321
BveI	ACCTGC	1 cut(s)	1768
Cac8I	GCNNGC	9 cut(s)	66, 154, 235, 735, 1525, 1675, 1765, 1889, 2403
CaiI	CAGNNNCTG	1 cut(s)	2345
CciNI	GCGGCCGC	1 cut(s)	94
CfoI	GCGC	3 cut(s)	409, 571, 1240
Cfr10I	RCCGGY	1 cut(s)	152
Cfr13I	GGNCC	4 cut(s)	599, 1274, 1633, 1895
ClaI	ATCGAT	1 cut(s)	202
CseI	GACGC	2 cut(s)	320, 1112
Csp6I	GTAC	8 cut(s)	542, 702, 1004, 1221, 1609, 1812, 1864, 2006
CviQI	GTAC	8 cut(s)	542, 702, 1004, 1221, 1609, 1812, 1864, 2006
DinI	GGCGCC	1 cut(s)	1239
DraI	TTTAAA	1 cut(s)	1666
DrdI	GACNNNNNNGTC	3 cut(s)	456, 2560, 2750
DriI	GACNNNNNGTC	1 cut(s)	2356
DseDI	GACNNNNNNGTC	3 cut(s)	456, 2560, 2750
EaeI	YGGCCR	3 cut(s)	12, 94, 294
EagI	CGGCCG	1 cut(s)	94
Eam1104I	CTCTTC	3 cut(s)	1008, 1401, 2823
Eam1105I	GACNNNNNGTC	1 cut(s)	2356
EarI	CTCTTC	3 cut(s)	1008, 1401, 2823
EciI	GGCGGA	1 cut(s)	1400
Ecl136II	GAGCTC	1 cut(s)	2382
EclXI	CGGCCG	1 cut(s)	94
Eco130I	CCWWGG	5 cut(s)	9, 159, 367, 590, 2652
Eco147I	AGGCCT	3 cut(s)	971, 1141, 2127
Eco24I	GRGCYC	1 cut(s)	2384
Eco31I	GGTCTC	2 cut(s)	266, 2343
Eco52I	CGGCCG	1 cut(s)	94
Eco53kI	GAGCTC	1 cut(s)	2382
Eco57I	CTGAAG	4 cut(s)	1473, 2061, 2616, 2648
Eco81I	CCTNAGG	1 cut(s)	70
Eco88I	CYCGRG	3 cut(s)	167, 788, 2166
EcoICRI	GAGCTC	1 cut(s)	2382
EcoRI	GAATTC	1 cut(s)	1725
EcoRII	CCWGG	2 cut(s)	1240, 1635
EcoT14I	CCWWGG	5 cut(s)	9, 159, 367, 590, 2652
EcoT38I	GRGCYC	1 cut(s)	2384
EgeI	GGCGCC	1 cut(s)	1239
EheI	GGCGCC	1 cut(s)	1239
ErhI	CCWWGG	5 cut(s)	9, 159, 367, 590, 2652
Esp3I	CGTCTC	1 cut(s)	117
FalI	AAGNNNNNCTT	4 cut(s)	1383, 1415, 2059, 2091
FaqI	GGGAC	3 cut(s)	1390, 1667, 2715
FauI	CCCGC	2 cut(s)	1726, 2417
FauNDI	CATATG	1 cut(s)	946
FbaI	TGATCA	1 cut(s)	1582
FblI	GTMKAC	1 cut(s)	1615
FokI	GGATG	6 cut(s)	674, 771, 1532, 1841, 1957, 2237
FriOI	GRGCYC	1 cut(s)	2384
FspBI	CTAG	4 cut(s)	761, 2009, 2328, 2541
GlaI	GCGC	3 cut(s)	408, 570, 1239
GsaI	CCCAGC	2 cut(s)	477, 636
GsuI	CTGGAG	2 cut(s)	1398, 2367
HaeII	RGCGCY	1 cut(s)	1241
HapII	CCGG	1 cut(s)	153
HgaI	GACGC	2 cut(s)	320, 1112
HhaI	GCGC	3 cut(s)	409, 571, 1240
Hin1I	GRCGYC	4 cut(s)	5, 312, 1123, 1238
Hin6I	GCGC	3 cut(s)	407, 569, 1238
HinP1I	GCGC	3 cut(s)	407, 569, 1238
HindIII	AAGCTT	2 cut(s)	1030, 1750
HinfI	GANTC	8 cut(s)	613, 1104, 1262, 1378, 1984, 2303, 2455, 2800
HpaII	CCGG	1 cut(s)	153
HphI	GGTGA	1 cut(s)	1256
Hpy166II	GTNNAC	6 cut(s)	422, 1340, 1541, 1616, 1814, 1940
Hpy8I	GTNNAC	6 cut(s)	422, 1340, 1541, 1616, 1814, 1940
Hpy99I	CGWCG	3 cut(s)	152, 314, 1887
HpyAV	CCTTC	7 cut(s)	390, 406, 844, 1094, 2061, 2138, 2569
HpyCH4IV	ACGT	5 cut(s)	457, 716, 961, 993, 1288
HpySE526I	ACGT	5 cut(s)	457, 716, 961, 993, 1288
Hsp92I	GRCGYC	4 cut(s)	5, 312, 1123, 1238
HspAI	GCGC	3 cut(s)	407, 569, 1238
KasI	GGCGCC	1 cut(s)	1237
KroI	GCCGGC	1 cut(s)	152
KroNI	GCCGGC	1 cut(s)	154
Ksp22I	TGATCA	1 cut(s)	1582
LguI	GCTCTTC	1 cut(s)	1401
LmnI	GCTCC	2 cut(s)	415, 919
LweI	GCATC	4 cut(s)	861, 1237, 1510, 1864
MaeI	CTAG	4 cut(s)	761, 2009, 2328, 2541
MaeII	ACGT	5 cut(s)	457, 716, 961, 993, 1288
MaeIII	GTNAC	9 cut(s)	496, 1225, 1690, 1852, 2071, 2234, 2480, 2611, 2644
MbiI	CCGCTC	1 cut(s)	124
MfeI	CAATTG	2 cut(s)	902, 2410
MflI	RGATCY	6 cut(s)	532, 744, 757, 2055, 2276, 2664
MhlI	GDGCHC	4 cut(s)	168, 1909, 1942, 2384
MlsI	TGGCCA	1 cut(s)	14
MluNI	TGGCCA	1 cut(s)	14
Mly113I	GGCGCC	1 cut(s)	1238
MlyI	GAGTC	2 cut(s)	1113, 1372
MmeI	TCCRAC	6 cut(s)	175, 677, 1304, 1686, 2170, 2581
Mox20I	TGGCCA	1 cut(s)	14
MroNI	GCCGGC	1 cut(s)	152
MroXI	GAANNNNTTC	1 cut(s)	386
MscI	TGGCCA	1 cut(s)	14
MslI	CAYNNNNRTG	3 cut(s)	161, 389, 608
Msp20I	TGGCCA	1 cut(s)	14
MspA1I	CMGCKG	4 cut(s)	31, 93, 1721, 2345
MspI	CCGG	1 cut(s)	153
MspR9I	CCNGG	2 cut(s)	1242, 1637
MunI	CAATTG	2 cut(s)	902, 2410
Mva1269I	GAATGC	2 cut(s)	1527, 1776
MvaI	CCWGG	2 cut(s)	1242, 1637
MvnI	CGCG	2 cut(s)	321, 2232
NaeI	GCCGGC	1 cut(s)	154
NarI	GGCGCC	1 cut(s)	1238
NcoI	CCATGG	1 cut(s)	9
NdeI	CATATG	1 cut(s)	946
NgoMIV	GCCGGC	1 cut(s)	152
NlaIV	GGNNCC	6 cut(s)	77, 157, 250, 601, 1239, 2278
NmuCI	GTSAC	1 cut(s)	2480
NotI	GCGGCCGC	1 cut(s)	94
NruI	TCGCGA	1 cut(s)	321
NspI	RCATGY	1 cut(s)	1547
NspV	TTCGAA	1 cut(s)	2464
OliI	CACNNNNGTG	1 cut(s)	161
PaeR7I	CTCGAG	1 cut(s)	167
PceI	AGGCCT	3 cut(s)	971, 1141, 2127
PciI	ACATGT	1 cut(s)	1543
PciSI	GCTCTTC	1 cut(s)	1401
PcsI	WCGNNNNNNNCGW	2 cut(s)	318, 454
PctI	GAATGC	2 cut(s)	1527, 1776
PdiI	GCCGGC	1 cut(s)	154
PdmI	GAANNNNTTC	1 cut(s)	386
PfeI	GAWTC	6 cut(s)	613, 1262, 1984, 2303, 2455, 2800
PflMI	CCANNNNNTGG	1 cut(s)	947
PleI	GAGTC	2 cut(s)	1112, 1372
PluTI	GGCGCC	1 cut(s)	1241
PpsI	GAGTC	2 cut(s)	1112, 1372
PscI	ACATGT	1 cut(s)	1543
PshBI	ATTAAT	2 cut(s)	206, 1326
Psp124BI	GAGCTC	1 cut(s)	2384
Psp1406I	AACGTT	2 cut(s)	716, 1288
Psp6I	CCWGG	2 cut(s)	1240, 1635
PspFI	CCCAGC	2 cut(s)	473, 632
PspGI	CCWGG	2 cut(s)	1240, 1635
PspN4I	GGNNCC	6 cut(s)	77, 157, 250, 601, 1239, 2278
PspPI	GGNCC	4 cut(s)	599, 1274, 1633, 1895
PsrI	GAACNNNNNNTAC	2 cut(s)	2629, 2661
PstI	CTGCAG	2 cut(s)	92, 2476
PstNI	CAGNNNCTG	1 cut(s)	2345
PsuI	RGATCY	6 cut(s)	532, 744, 757, 2055, 2276, 2664
PvuII	CAGCTG	1 cut(s)	2345
RruI	TCGCGA	1 cut(s)	321
RsaI	GTAC	8 cut(s)	543, 703, 1005, 1222, 1610, 1813, 1865, 2007
RsaNI	GTAC	8 cut(s)	542, 702, 1004, 1221, 1609, 1812, 1864, 2006
RseI	CAYNNNNRTG	3 cut(s)	161, 389, 608
SacI	GAGCTC	1 cut(s)	2384
SapI	GCTCTTC	1 cut(s)	1401
Sau96I	GGNCC	4 cut(s)	599, 1274, 1633, 1895
ScaI	AGTACT	2 cut(s)	1005, 2007
SchI	GAGTC	2 cut(s)	1113, 1372
ScrFI	CCNGG	2 cut(s)	1242, 1637
SduI	GDGCHC	4 cut(s)	168, 1909, 1942, 2384
SfaNI	GCATC	4 cut(s)	861, 1237, 1510, 1864
SfcI	CTRYAG	3 cut(s)	88, 1303, 2472
SfoI	GGCGCC	1 cut(s)	1239
Sfr274I	CTCGAG	1 cut(s)	167
SfuI	TTCGAA	1 cut(s)	2464
SlaI	CTCGAG	1 cut(s)	167
SmiMI	CAYNNNNRTG	3 cut(s)	161, 389, 608
SmlI	CTYRAG	2 cut(s)	167, 673
SmoI	CTYRAG	2 cut(s)	167, 673
SpeI	ACTAGT	1 cut(s)	2540
SseBI	AGGCCT	3 cut(s)	971, 1141, 2127
SspDI	GGCGCC	1 cut(s)	1237
SspI	AATATT	1 cut(s)	982
SspMI	CTAG	4 cut(s)	761, 2009, 2328, 2541
SstI	GAGCTC	1 cut(s)	2384
StuI	AGGCCT	3 cut(s)	971, 1141, 2127
StyD4I	CCNGG	2 cut(s)	1240, 1635
StyI	CCWWGG	5 cut(s)	9, 159, 367, 590, 2652
TaiI	ACGT	5 cut(s)	460, 719, 964, 996, 1291
TaqI	TCGA	7 cut(s)	168, 202, 216, 448, 2043, 2269, 2464
TatI	WGTACW	6 cut(s)	541, 1003, 1220, 1811, 1863, 2005
TauI	GCSGC	6 cut(s)	96, 99, 127, 299, 302, 1350
TfiI	GAWTC	6 cut(s)	613, 1262, 1984, 2303, 2455, 2800
TscAI	CASTG	2 cut(s)	940, 1971
TseFI	GTSAC	1 cut(s)	2480
Tsp45I	GTSAC	1 cut(s)	2480
TspDTI	ATGAA	9 cut(s)	379, 501, 1038, 1146, 1282, 1344, 1944, 2301, 2424
TspGWI	ACGGA	3 cut(s)	1957, 2296, 2756
TspRI	CASTG	2 cut(s)	940, 1971
Van91I	CCANNNNNTGG	1 cut(s)	947
VneI	GTGCAC	1 cut(s)	1938
VspI	ATTAAT	2 cut(s)	206, 1326
XapI	RAATTY	5 cut(s)	185, 1725, 1782, 1837, 2431
XbaI	TCTAGA	1 cut(s)	760
XceI	RCATGY	1 cut(s)	1547
XhoI	CTCGAG	1 cut(s)	167
XmiI	GTMKAC	1 cut(s)	1615
XmnI	GAANNNNTTC	1 cut(s)	386
XspI	CTAG	4 cut(s)	761, 2009, 2328, 2541
ZrmI	AGTACT	2 cut(s)	1005, 2007

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.