Rroxscaffold_2G00079710

Polyribonucleotide nucleotidyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004785
Physical Location & Seq
Reverse (-)
2682829 .. 2695277
12449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00079710.1

Sequence Viewer

Length: 5001 bp
ATGGCCTCCGTGGCCCACCGCGCCAACCCACTCGTCCACCACCTCCCTCATTTCCTCACATGGCGATCCCTACGCTTCCGCACCATCTGCAGCGGCCGCATGGGCTTCTCCTCCCACTCTCAACCCGAACCCGAACCCGAAACCCGTACTTTGCCCGGAACCAAGCTTCTCGAAACCTTCACAGAACAATTCGAGATCGGATCGCGCCTCATCACTCTCGAGACCGGAAAGATCGCACGTTTCGCTAACGGCGCCGTCGTTTTGGGCATGGACGAGACCAGAGTTCTCTCCACCGTCTGCTCCGCCAAGGGTGATGCCGTTAAAGACTTTTTGCCCCTCACCGTTGATTATCAAGAGAAACAATTTGCGCAAGGTATTATACCAAACACATTCATGAGAAGGGAGGGTGCACCGAAAGAGCGTGAGCTCTTGTGTGGTCGTTTGATTGATCGGCCTATTCGTCCGTTGTTTCCTGCAGGATTTTTTCATGAGGTTCAGGTGATGGCAAGTGTTCTTTCTTCTGATGGAAAGCAAGATCCAGATGTAATGGCAGCTAATGCTACATCTGCTGCTCTTATGTTATCAGACATTCCGTGGGCAGGCCCCATTGGAATGATTCGCATTGGGAGGATCGAGGGGAAGTTTATTGTTAACCCAACTATGGATGAGCTTAGCTTAAGTGATCTCAACTTAGTGTATGCATGTACAAGGGACAAAACGTTAATGATTGATGTGCAAGCTCGTGAGATTTCAGAAAAAGATCTAGAAGCTGGGATGAGATTGGCTCATCCTGAAGCAGTTAAATATCTTGAACCTCAAATCAGACTGGCTGCTAAAGCTGGCAAGCATAAGAAGGAATATAAATTGTCACTGATGCCAGAGAGAACATTGGAAAAAGTCGCCCAATTGGCAGAAGCTCCCATAGAAGAAGTTTTCACTGATCCTAAGTATGGAAAGTTTGAACGTGGAGAAGCCCTGGAGAATATTGCGCAAGATGTGAGAAAAGCACTTGAAGAAGAATGTGATGAAGAAAACTTGAGACTCTTACCGAAAGCAGTAGACACAGTGAGGAAAAAGGTTGTCCGTAGAAGAATAATTGCGGAAGGATCAAGACTTGATGGGAGGCGACTGGATGAAGTTAGACCTGTGTACTGTGAATCTGGTACTCTGTACCGTAACACTTGGGGCACCTGGCGATGCACAACGCTTGGAATCCGTGGTGGGCCCCCCATCAAAGCGCTTCATGCTTCACTACAGTTTCCCCCATTCTGTATAAATGAGGTCGGTAAACATGGTGGCTTGAACAGGCGTGAAGTTGGTCATGGAACTCTTGCGGAAAAAGCTCTGCTTGCTGTGTTACCTCCTGAAGATGACTTTCCATACACTGTTCGTATCAATTCTGAAGTAATGGCTTCTGATGGTTCAACATCTATGGCTTCTGTCTGTGGAGGCAGTATGGCTTTGATGGATGCTGGCATTCCACTGCGCAAACATGTAGCTGGTGTCTCCGTAGGTCTTGTTAGTGAAGTTGATCAATCAACTGGCAAAATAAAGGAATACCGTATATTGACTGACATTCTGGGTCTGGAAGATCATTTGGGGGACATGGACTTCAAAATTGTTGGCACGCAAGATGGAATCACCGCAATTCAGTTGGATATAAAACCTGCTGGAATTCCTTTAGATATTGTTTGTGAAAGCTTGGAGGCGGCACGTAAAGGTCGTATCCAAATTCTTGATCACATGGAGAGAGAGATCAGTGCACCACGCACTCAAGATGATAGAAATTCTCCTCGATTAGCTACGTTGAAGTACAGCAATGATGCACTTCGTCGCTTGCTTGGGCCTCTGGGTGCTCTTAAAAGAAAAATAGAAGACGAAACAGGTACACGGATCTCTGTAAGTGATGGAACACTCACCATAGTTGCTAAGAATCAATCTGCAATGGACAAAGTACTCGAAAAGGTTGATTTTGTACTTGGACGTGAAATTGAAATTGGTGGGATCTATAAAGGGACTGTAGCTTCAATCAAAGAATATGGTGCATTCGTGGAGTTTAATGGAGGGCAACAAGGCCTTCTACACATTTCCGAGTTGTCACATGAACCGGTGTCACGGATTTCAGATGTAGTATCTGTAGGCCAGCAGCTCTCTTTGATGTGCATAGGCCAGGATGTACGTGGTAACATTAAGTTGTCCCTGAAAGCTACATTACCTCGACCACAATCTCAGACAAAGAATTTGGCTGTGGAATCTGTTCCATCTATGAAACAAGCTCCTAGTGTTTGGGCAGCTACTGGAGATGTGTCTAGTGGTGAAGAAAAGCATAGCCCTTTGTCTGAGGAATTGCCAGTGAGTAAATATGAAGTTAATGCAATGAAATCCTCTACTTCCTCTAGTCCACCAGTTCTAGTCAGAAGTGCTGAAGAGTGCGACGAGGAGGAAAGATCTACTGGTTTTGTTCAAAATTCTAAAACTACTTCTGAACCCATTGGTGGTTTGAAACCAGAGCGCAAGACTGTTCAAACTACTCCTAAGACATCAAAATCTTTCTTACAAAAGGAACGAGATAAAGATGAGAAGGGACACAATATTGCATCCCAAAACCACTCTAGTAATGATTTGAGTAACAAGGAAGACCCTGTTACTGCAAAAAGTCTGAAGCTAGGAACAAAGGTTACTGCCAAGGTTTATCAGATCCGCACAGGTGGGCTGGTGCTTGATCTGGGTGGGGGACTTCGAGGAATGTATCGGTTTGAGAATATTGTCTTCCTAGCACCATCAGTCTTGGTCTACTTTGTTGTCTTCTCATACGATCTACTGTCTAAAGCAGCCTCCATCTTTCTGACATTTCCATATGTTGTTGCCTTGCCACTCATCCAGTCTCTCAATGTCCTGCCAATGCTAAGAAGGCCTTTGATGTCAACGATGAGTTGCGAGTTGAGTGTGTTAGTTTTTCTAGCAAGGGGATTCCGTGGAATAGCTGTGTGTTTTAACAGTCACCACAATTTTCCCAAATTGACATTGATGTCTCGATTAGTTAGGCATTTTTTTGGGTGGGCAAAAATAATGGAAGCACCAAATGCTCAGTTTTCTCTTCCTATCAATGTAGGCATATCTTCAGTAATCTTATCCAAGTGTTATAGTTATATCGATGACCTGGCAGATATGAAGGAGGACCCTCGAACAGAGTTGGTTGCCCGCGCATGCAGCCATACGCGTGCAATTCATACAGCAAGTTACCATGTTACCTTCAACAAGGTGGTGGCTGAGATGGATAAAAAAATGCTCCGTAACAACTTTGGGACTGCGGAAGAGGGTGAGCCTCCTGACCGGCTTTATATGTTGGCGCAATGCATGACAGATCTTTCGGTCAATGAATGTAAGACGTGCTATGATTTGAGTAAGCATGCCTTGGCTGGCTGCCTTCCTAACACAGGAGGTCGTGCTTTATTTGATGGTTGCTTTGTAAGGGCTGAAAATTACAGCTTCTTTGATGAGACATTCCAAGATAAAGATTTCAAGCATTGCAGCGACAATGCCGAGTTCAATCAGAAATTTAACCAAACAGCAGAGAAAGTGATAAAAAAACTTGTGAAGTTGGCTGAGCATAATGATGGATTTGCAATCGCCAAAGATAGCTCAAATGGTTTTACAGTGTTTGCTATGGCTAATTGCTGGAGGAGTCTAGACAGTAGTAAGTGTTCTTCATGTGTAGAAAATGCAGCCAACAGTGCAATTTCATGCTTGCCATCGTCTGAGGGCCGTGTGCTCAATTCTGGCTGCGTTTTACGTTACTCTGATTATGACTTTGGCAATAACCCTCGAGCCCTCGAAATCAAAGAATCTGCACTAAAGACTGCCTCATATGTTATTGTTGCGTTGGCCTTGTTGGCCTTGTTCGCATTGATTATTATGCTCGGAATTTGTGCGGCTAAGGGAAAGGAGAGAGAGATAGTTTTTAACCAAAGCTCAGAGTTCCAGAATTTTAAGTACTCAGCACTAGTGAAAGCAACCGAGTGCTTCGATGAAGCCCATAAGCTTGGTCAAGGAGCATATGGTGAAGTATTTAGGACCCTAACAAGCGGTATCGAGCTCCAAATCATGGGGCGGGGAGCGCTCTACCACAATACTCATCTACACCGCTGCGTCCACGACCATAGTAATTTTGGCCTCTACGGCGGGGGTTATAGTCGTTCGACCGTTATCGTCTTCACGTCGCACTCGGTTGAGCGCCACCGTACGTCCGCCTCCTCACGCCGACCATCTACTTTCCCCACCGAGCTCTTATTCTCCACCATCGCCTCCTTATTCCGCTCACTATTTTCATCATCAATTTATTCCTCATGGACATCCTCGGCAGCCTACACCACTTCTAAACAATCACCCAGCTATTGTTCCATCTCCTCCAAATGCAAATGGCGGTGGGCTTGCTATGCCAATAGACAAGACGCCTGTGCTTTGTCAATTCGGGGTGAACTTGGAATCGGATCTTCCATAGTCATAGACCCGACTCGGTGTAGTGGCAATGGATTGTCGTCGTCGGATCGAGCATATATTAATCCTTCCACTCAATCTCTTGGTGTGGAGGATTATGTTCGGTTGCCGGAGCAGGACGATGGTGGTCTAGTTGAAGATAAGGAGCAGCTTAGCAATGGAATGCCAGAATTTTATGCCCTCCAACTATGCATCGAGGCTATTGAAACAATTGATGCCACCGACGGCGTGATTAAGAGATGCTTGAACATCAACTTACCCAATAAATTTGCTAGTGAAACGATTGAGAATGGCTCTAAGTTCGAAGGATCAAATGCAAAGAATTCGGAATATGTGCAAGATGATGAAGTTGATCCATTAGAGAATGAGCATAGTGATGGGTTTGATGATAAGGAAAATGATGTCAAACAATCTGATTTCGATGAGAATTTGAGCATCTACCAGTCAAGTCATTTAAGTGGAGGCACTTGTTGTCTCTTCGGATATGCATGCATTGGAAGCTAG

Protein Analysis

1666

Amino Acids

183.64

Weight (kDa)

6.27

Isoelectric Point (pI)

43.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_PH PF01138 68 - 198 1.2e-24 3' exoribonuclease family, domain 1
RNase_PH_C PF03725 201 - 263 2.6e-11 3' exoribonuclease family, domain 2
PNPase PF03726 297 - 376 4.2e-16 Polyribonucleotide nucleotidyltransferase, RNA binding domain
RNase_PH PF01138 412 - 494 2.2e-13 3' exoribonuclease family, domain 1
S1 PF00575 664 - 735 2.4e-11 S1 RNA binding domain
Stress-antifung PF01657 1070 - 1162 8.5e-12 Salt stress response/antifungal
Stress-antifung PF01657 1177 - 1269 4e-12 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3028
Acc16I TGCGCA 3 cut(s) 369, 990, 1487
Acc36I ACCTGC 1 cut(s) 1675
AccB1I GGYRCC 2 cut(s) 251, 1187
AccB7I CCANNNNNTGG 1 cut(s) 4105
AccBSI CCGCTC 1 cut(s) 4317
AccI GTMKAC 2 cut(s) 1059, 2793
AccII CGCG 4 cut(s) 21, 205, 3204, 3220
AclI AACGTT 1 cut(s) 719
AcoI YGGCCR 1 cut(s) 94
AcuI CTGAAG 6 cut(s) 813, 1386, 1422, 2445, 2681, 3105
AcyI GRCGYC 2 cut(s) 252, 4452
AfeI AGCGCT 2 cut(s) 1239, 4119
AfiI CCNNNNNNNGG 6 cut(s) 599, 661, 950, 2495, 3437, 4105
AflII CTTAAG 1 cut(s) 676
AflIII ACRYGT 2 cut(s) 1492, 3218
AgeI ACCGGT 1 cut(s) 2107
AhlI ACTAGT 1 cut(s) 4003
AjiI CACGTC 3 cut(s) 1985, 3390, 4218
AjnI CCWGG 4 cut(s) 975, 1190, 2169, 3159
AjuI GAANNNNNNNTTGG 2 cut(s) 1980, 2012
Alw21I GWGCWC 7 cut(s) 412, 429, 1765, 1858, 3774, 4098, 4287
Alw26I GTCTC 8 cut(s) 215, 269, 1033, 1510, 2889, 3036, 3494, 4976
Alw44I GTGCAC 2 cut(s) 408, 1761
AlwNI CAGNNNCTG 1 cut(s) 2297
Ama87I CYCGRG 2 cut(s) 218, 3825
Aor51HI AGCGCT 2 cut(s) 1239, 4119
ApaI GGGCCC 1 cut(s) 1227
ApaLI GTGCAC 2 cut(s) 408, 1761
ArsI GACNNNNNNTTYG 4 cut(s) 2224, 2256, 3352, 3384
AseI ATTAAT 1 cut(s) 4560
AsiGI ACCGGT 1 cut(s) 2107
Asp700I GAANNNNTTC 1 cut(s) 2256
AspS9I GGNCC 8 cut(s) 13, 602, 1223, 1224, 1844, 3178, 3763, 4074
AsuC2I CCSGG 1 cut(s) 156
AsuII TTCGAA 1 cut(s) 4800
AvaI CYCGRG 2 cut(s) 218, 3825
AvaII GGWCC 2 cut(s) 3178, 4074
BaeGI GKGCMC 4 cut(s) 412, 1190, 1227, 1765
BanI GGYRCC 2 cut(s) 251, 1187
BanII GRGCYC 5 cut(s) 429, 1227, 3832, 4098, 4287
BarI GAAGNNNNNNTAC 2 cut(s) 3691, 3723
BauI CACGAG 1 cut(s) 741
BbsI GAAGAC 5 cut(s) 1881, 2643, 2761, 2797, 4204
Bbv12I GWGCWC 7 cut(s) 412, 429, 1765, 1858, 3774, 4098, 4287
BceAI ACGGC 6 cut(s) 239, 265, 302, 3750, 4195, 4738
BcgI CGANNNNNNTGC 2 cut(s) 4803, 4837
BciT130I CCWGG 4 cut(s) 977, 1192, 2171, 3161
BciVI GTATCC 1 cut(s) 1736
BclI TGATCA 2 cut(s) 1531, 1738
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 8 cut(s) 215, 269, 1033, 1510, 2889, 3036, 3494, 4976
BcuI ACTAGT 1 cut(s) 4003
BfmI CTRYAG 5 cut(s) 88, 474, 1253, 2019, 2136
BfoI RGCGCY 4 cut(s) 255, 1241, 4121, 4237
BfrI CTTAAG 1 cut(s) 676
BfuAI ACCTGC 1 cut(s) 1675
BfuI GTATCC 1 cut(s) 1736
BglI GCCNNNNNGGC 5 cut(s) 11, 102, 908, 3893, 4179
BglII AGATCT 3 cut(s) 760, 2447, 3364
BlpI GCTNAGC 3 cut(s) 671, 3606, 4649
BmcAI AGTACT 2 cut(s) 1956, 3995
Bme1390I CCNGG 5 cut(s) 156, 977, 1192, 2171, 3161
Bme18I GGWCC 2 cut(s) 3178, 4074
BmeT110I CYCGRG 2 cut(s) 218, 3825
BmgBI CACGTC 3 cut(s) 1985, 3390, 4218
BmgT120I GGNCC 8 cut(s) 13, 602, 1223, 1224, 1844, 3178, 3763, 4074
BmiI GGNNCC 8 cut(s) 160, 253, 604, 1189, 1225, 1226, 3180, 4076
BmrFI CCNGG 5 cut(s) 156, 977, 1192, 2171, 3161
BpiI GAAGAC 5 cut(s) 1881, 2643, 2761, 2797, 4204
BplI GAGNNNNNCTC 4 cut(s) 769, 801, 4775, 4807
BpmI CTGGAG 3 cut(s) 998, 2319, 3700
Bpu10I CCTNAGC 1 cut(s) 3936
Bpu1102I GCTNAGC 3 cut(s) 671, 3606, 4649
Bpu14I TTCGAA 1 cut(s) 4800
BpuEI CTTGAG 2 cut(s) 1057, 1758
BpuMI CCSGG 1 cut(s) 156
Bsa29I ATCGAT 1 cut(s) 3153
BsaAI YACGTR 2 cut(s) 1715, 2180
BsaHI GRCGYC 2 cut(s) 252, 4452
BsaI GGTCTC 2 cut(s) 215, 269
BsaJI CCNNGG 9 cut(s) 9, 306, 593, 975, 1216, 2685, 2974, 3414, 4356
BsaWI WCCGGW 2 cut(s) 224, 2107
Bsc4I CCNNNNNNNGG 6 cut(s) 599, 661, 950, 2495, 3437, 4105
Bse118I RCCGGY 2 cut(s) 2107, 3333
Bse1I ACTGG 9 cut(s) 831, 1134, 1546, 2302, 2351, 2405, 2458, 2881, 4939
Bse3DI GCAATG 7 cut(s) 1825, 1950, 2382, 3359, 3526, 4534, 4660
BseBI CCWGG 4 cut(s) 977, 1192, 2171, 3161
BseCI ATCGAT 1 cut(s) 3153
BseDI CCNNGG 9 cut(s) 9, 306, 593, 975, 1216, 2685, 2974, 3414, 4356
BseGI GGATG 9 cut(s) 670, 780, 787, 1138, 1474, 2179, 2597, 2877, 4352
BseLI CCNNNNNNNGG 6 cut(s) 599, 661, 950, 2495, 3437, 4105
BseMI GCAATG 7 cut(s) 1825, 1950, 2382, 3359, 3526, 4534, 4660
BseMII CTCAG 8 cut(s) 2243, 2331, 3101, 3261, 3597, 3750, 3987, 4011
BseNI ACTGG 9 cut(s) 831, 1134, 1546, 2302, 2351, 2405, 2458, 2881, 4939
BseRI GAGGAG 6 cut(s) 100, 1782, 2453, 3697, 4243, 4396
BseSI GKGCMC 4 cut(s) 412, 1190, 1227, 1765
BseX3I CGGCCG 1 cut(s) 94
BseYI CCCAGC 2 cut(s) 770, 4388
BsgI GTGCAG 1 cut(s) 3834
Bsh1236I CGCG 4 cut(s) 21, 205, 3204, 3220
Bsh1285I CGRYCG 2 cut(s) 97, 4203
BshNI GGYRCC 2 cut(s) 251, 1187
BshTI ACCGGT 1 cut(s) 2107
BshVI ATCGAT 1 cut(s) 3153
BsiEI CGRYCG 2 cut(s) 97, 4203
BsiHKAI GWGCWC 7 cut(s) 412, 429, 1765, 1858, 3774, 4098, 4287
BsiHKCI CYCGRG 2 cut(s) 218, 3825
BsiSI CCGG 5 cut(s) 156, 225, 2108, 3334, 4607
BsiWI CGTACG 1 cut(s) 4241
BslFI GGGAC 7 cut(s) 725, 1616, 2029, 2182, 2598, 2748, 3319
BslI CCNNNNNNNGG 6 cut(s) 599, 661, 950, 2495, 3437, 4105
BsmAI GTCTC 8 cut(s) 215, 269, 1033, 1510, 2889, 3036, 3494, 4976
BsmFI GGGAC 7 cut(s) 725, 1616, 2029, 2182, 2598, 2748, 3319
BsmI GAATGC 3 cut(s) 1476, 2045, 4665
Bso31I GGTCTC 2 cut(s) 215, 269
BsoBI CYCGRG 2 cut(s) 218, 3825
Bsp119I TTCGAA 1 cut(s) 4800
Bsp120I GGGCCC 1 cut(s) 1223
Bsp1407I TGTACA 1 cut(s) 704
Bsp1720I GCTNAGC 3 cut(s) 671, 3606, 4649
BspCNI CTCAG 8 cut(s) 2242, 2332, 3100, 3262, 3598, 3751, 3986, 4010
BspDI ATCGAT 1 cut(s) 3153
BspFNI CGCG 4 cut(s) 21, 205, 3204, 3220
BspHI TCATGA 2 cut(s) 393, 487
BspLI GGNNCC 8 cut(s) 160, 253, 604, 1189, 1225, 1226, 3180, 4076
BspMAI CTGCAG 2 cut(s) 92, 478
BspMI ACCTGC 1 cut(s) 1675
BspT104I TTCGAA 1 cut(s) 4800
BspT107I GGYRCC 2 cut(s) 251, 1187
BspTI CTTAAG 1 cut(s) 676
BspTNI GGTCTC 2 cut(s) 215, 269
BsrBI CCGCTC 1 cut(s) 4317
BsrDI GCAATG 7 cut(s) 1825, 1950, 2382, 3359, 3526, 4534, 4660
BsrFI RCCGGY 2 cut(s) 2107, 3333
BsrGI TGTACA 1 cut(s) 704
BsrI ACTGG 9 cut(s) 831, 1134, 1546, 2302, 2351, 2405, 2458, 2881, 4939
BssAI RCCGGY 2 cut(s) 2107, 3333
BssECI CCNNGG 9 cut(s) 9, 306, 593, 975, 1216, 2685, 2974, 3414, 4356
BssNI GRCGYC 2 cut(s) 252, 4452
BssSI CACGAG 1 cut(s) 741
BssT1I CCWWGG 3 cut(s) 306, 2685, 3414
Bst2BI CACGAG 1 cut(s) 741
Bst2UI CCWGG 4 cut(s) 977, 1192, 2171, 3161
Bst6I CTCTTC 4 cut(s) 2421, 3102, 3309, 4979
BstACI GRCGYC 2 cut(s) 252, 4452
BstAFI CTTAAG 1 cut(s) 676
BstAPI GCANNNNNTGC 3 cut(s) 87, 557, 3083
BstAUI TGTACA 1 cut(s) 704
BstBAI YACGTR 2 cut(s) 1715, 2180
BstBI TTCGAA 1 cut(s) 4800
BstDSI CCRYGG 4 cut(s) 9, 593, 1216, 2974
BstENI CCTNNNNNAGG 1 cut(s) 3435
BstF5I GGATG 9 cut(s) 670, 780, 787, 1138, 1474, 2179, 2597, 2877, 4352
BstFNI CGCG 4 cut(s) 21, 205, 3204, 3220
BstH2I RGCGCY 4 cut(s) 255, 1241, 4121, 4237
BstMAI GTCTC 8 cut(s) 215, 269, 1033, 1510, 2889, 3036, 3494, 4976
BstMCI CGRYCG 2 cut(s) 97, 4203
BstNI CCWGG 4 cut(s) 977, 1192, 2171, 3161
BstNSI RCATGY 5 cut(s) 705, 1496, 3210, 3413, 4989
BstSCI CCNGG 5 cut(s) 154, 975, 1190, 2169, 3159
BstSFI CTRYAG 5 cut(s) 88, 474, 1253, 2019, 2136
BstSLI GKGCMC 4 cut(s) 412, 1190, 1227, 1765
BstUI CGCG 4 cut(s) 21, 205, 3204, 3220
BstV2I GAAGAC 5 cut(s) 1881, 2643, 2761, 2797, 4204
BstX2I RGATCY 8 cut(s) 535, 760, 1893, 2004, 2447, 2697, 3364, 4490
BstXI CCANNNNNNTGG 1 cut(s) 4043
BstYI RGATCY 8 cut(s) 535, 760, 1893, 2004, 2447, 2697, 3364, 4490
BstZI CGGCCG 1 cut(s) 94
Bsu15I ATCGAT 1 cut(s) 3153
BsuI GTATCC 1 cut(s) 1736
BsuTUI ATCGAT 1 cut(s) 3153
BtgI CCRYGG 4 cut(s) 9, 593, 1216, 2974
BtgZI GCGATG 2 cut(s) 1210, 4285
BtrI CACGTC 3 cut(s) 1985, 3390, 4218
BtsCI GGATG 9 cut(s) 670, 780, 787, 1138, 1474, 2179, 2597, 2877, 4352
BtsI GCAGTG 1 cut(s) 1481
BtsIMutI CAGTG 9 cut(s) 869, 936, 1071, 1383, 1481, 1765, 2358, 3663, 3739
BveI ACCTGC 1 cut(s) 1675
CaiI CAGNNNCTG 1 cut(s) 2297
CciI TCATGA 2 cut(s) 393, 487
CciNI GCGGCCGC 1 cut(s) 94
Cfr10I RCCGGY 2 cut(s) 2107, 3333
Cfr13I GGNCC 8 cut(s) 13, 602, 1223, 1224, 1844, 3178, 3763, 4074
ClaI ATCGAT 1 cut(s) 3153
CseI GACGC 2 cut(s) 4138, 4460
CspAI ACCGGT 1 cut(s) 2107
DinI GGCGCC 1 cut(s) 253
DrdI GACNNNNNNGTC 1 cut(s) 3028
DseDI GACNNNNNNGTC 1 cut(s) 3028
EaeI YGGCCR 1 cut(s) 94
EagI CGGCCG 1 cut(s) 94
Eam1104I CTCTTC 4 cut(s) 2421, 3102, 3309, 4979
EarI CTCTTC 4 cut(s) 2421, 3102, 3309, 4979
EciI GGCGGA 2 cut(s) 292, 4237
Ecl136II GAGCTC 3 cut(s) 427, 4096, 4285
EclXI CGGCCG 1 cut(s) 94
Eco130I CCWWGG 3 cut(s) 306, 2685, 3414
Eco147I AGGCCT 2 cut(s) 2076, 2914
Eco24I GRGCYC 5 cut(s) 429, 1227, 3832, 4098, 4287
Eco31I GGTCTC 2 cut(s) 215, 269
Eco47I GGWCC 2 cut(s) 3178, 4074
Eco47III AGCGCT 2 cut(s) 1239, 4119
Eco52I CGGCCG 1 cut(s) 94
Eco53kI GAGCTC 3 cut(s) 427, 4096, 4285
Eco57I CTGAAG 6 cut(s) 813, 1386, 1422, 2445, 2681, 3105
Eco88I CYCGRG 2 cut(s) 218, 3825
EcoICRI GAGCTC 3 cut(s) 427, 4096, 4285
EcoNI CCTNNNNNAGG 1 cut(s) 3435
EcoO109I RGGNCCY 4 cut(s) 602, 1224, 3178, 4074
EcoRI GAATTC 2 cut(s) 1674, 4819
EcoRII CCWGG 4 cut(s) 975, 1190, 2169, 3159
EcoT14I CCWWGG 3 cut(s) 306, 2685, 3414
EcoT22I ATGCAT 5 cut(s) 703, 3359, 4691, 4987, 4991
EcoT38I GRGCYC 5 cut(s) 429, 1227, 3832, 4098, 4287
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
ErhI CCWWGG 3 cut(s) 306, 2685, 3414
FalI AAGNNNNNCTT 8 cut(s) 1332, 1364, 2899, 2931, 3398, 3430, 4724, 4756
FaqI GGGAC 7 cut(s) 725, 1616, 2029, 2182, 2598, 2748, 3319
FauI CCCGC 3 cut(s) 3209, 4104, 4175
FauNDI CATATG 3 cut(s) 2857, 3868, 4057
FbaI TGATCA 2 cut(s) 1531, 1738
FblI GTMKAC 2 cut(s) 1059, 2793
FokI GGATG 9 cut(s) 677, 774, 787, 1145, 1481, 2186, 2584, 2864, 4339
FriOI GRGCYC 5 cut(s) 429, 1227, 3832, 4098, 4287
FspI TGCGCA 3 cut(s) 369, 990, 1487
GsaI CCCAGC 2 cut(s) 774, 4392
GsuI CTGGAG 3 cut(s) 998, 2319, 3700
HaeII RGCGCY 4 cut(s) 255, 1241, 4121, 4237
HapII CCGG 5 cut(s) 156, 225, 2108, 3334, 4607
HgaI GACGC 2 cut(s) 4138, 4460
Hin1I GRCGYC 2 cut(s) 252, 4452
HincII GTYRAC 2 cut(s) 652, 2925
HindII GTYRAC 2 cut(s) 652, 2925
HindIII AAGCTT 3 cut(s) 164, 1699, 4040
HpaI GTTAAC 1 cut(s) 652
HpaII CCGG 5 cut(s) 156, 225, 2108, 3334, 4607
Hpy99I CGWCG 7 cut(s) 260, 1836, 2438, 4222, 4543, 4546, 4724
Hsp92I GRCGYC 2 cut(s) 252, 4452
KasI GGCGCC 1 cut(s) 251
Ksp22I TGATCA 2 cut(s) 1531, 1738
KspAI GTTAAC 1 cut(s) 652
LmnI GCTCC 9 cut(s) 305, 922, 2281, 3294, 4052, 4101, 4115, 4609, 4642
MbiI CCGCTC 1 cut(s) 4317
MfeI CAATTG 2 cut(s) 905, 4707
MflI RGATCY 8 cut(s) 535, 760, 1893, 2004, 2447, 2697, 3364, 4490
MluI ACGCGT 1 cut(s) 3218
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 3 cut(s) 1035, 3694, 4507
MmeI TCCRAC 3 cut(s) 1635, 4524, 4705
Mph1103I ATGCAT 5 cut(s) 703, 3359, 4691, 4987, 4991
MroXI GAANNNNTTC 1 cut(s) 2256
MslI CAYNNNNRTG 6 cut(s) 392, 611, 3219, 3615, 4872, 4953
MspA1I CMGCKG 2 cut(s) 93, 4146
MspCI CTTAAG 1 cut(s) 676
MspI CCGG 5 cut(s) 156, 225, 2108, 3334, 4607
MspR9I CCNGG 5 cut(s) 156, 977, 1192, 2171, 3161
MunI CAATTG 2 cut(s) 905, 4707
Mva1269I GAATGC 3 cut(s) 1476, 2045, 4665
MvaI CCWGG 4 cut(s) 977, 1192, 2171, 3161
MvnI CGCG 4 cut(s) 21, 205, 3204, 3220
NarI GGCGCC 1 cut(s) 252
NciI CCSGG 1 cut(s) 156
NdeI CATATG 3 cut(s) 2857, 3868, 4057
NlaIV GGNNCC 8 cut(s) 160, 253, 604, 1189, 1225, 1226, 3180, 4076
NmeAIII GCCGAG 2 cut(s) 3568, 4337
NmuCI GTSAC 4 cut(s) 867, 2097, 2112, 2999
NotI GCGGCCGC 1 cut(s) 94
NsbI TGCGCA 3 cut(s) 369, 990, 1487
NsiI ATGCAT 5 cut(s) 703, 3359, 4691, 4987, 4991
NspI RCATGY 5 cut(s) 705, 1496, 3210, 3413, 4989
NspV TTCGAA 1 cut(s) 4800
PaeI GCATGC 3 cut(s) 3210, 3413, 4989
PaeR7I CTCGAG 2 cut(s) 218, 3825
PagI TCATGA 2 cut(s) 393, 487
PceI AGGCCT 2 cut(s) 2076, 2914
PciI ACATGT 1 cut(s) 1492
PcsI WCGNNNNNNNCGW 3 cut(s) 255, 457, 1720
PctI GAATGC 3 cut(s) 1476, 2045, 4665
PdmI GAANNNNTTC 1 cut(s) 2256
PfeI GAWTC 9 cut(s) 616, 1157, 1212, 1638, 1933, 2252, 2970, 3845, 4485
Pfl23II CGTACG 1 cut(s) 4241
PflMI CCANNNNNTGG 1 cut(s) 4105
PinAI ACCGGT 1 cut(s) 2107
PleI GAGTC 3 cut(s) 1035, 3693, 4507
PluTI GGCGCC 1 cut(s) 255
PpsI GAGTC 3 cut(s) 1035, 3693, 4507
Ppu21I YACGTR 2 cut(s) 1715, 2180
PpuMI RGGWCCY 2 cut(s) 3178, 4074
PscI ACATGT 1 cut(s) 1492
PshBI ATTAAT 1 cut(s) 4560
Psp124BI GAGCTC 3 cut(s) 429, 4098, 4287
Psp1406I AACGTT 1 cut(s) 719
Psp5II RGGWCCY 2 cut(s) 3178, 4074
Psp6I CCWGG 4 cut(s) 975, 1190, 2169, 3159
PspFI CCCAGC 2 cut(s) 770, 4388
PspGI CCWGG 4 cut(s) 975, 1190, 2169, 3159
PspLI CGTACG 1 cut(s) 4241
PspN4I GGNNCC 8 cut(s) 160, 253, 604, 1189, 1225, 1226, 3180, 4076
PspOMI GGGCCC 1 cut(s) 1223
PspPI GGNCC 8 cut(s) 13, 602, 1223, 1224, 1844, 3178, 3763, 4074
PspPPI RGGWCCY 2 cut(s) 3178, 4074
PspXI VCTCGAGB 1 cut(s) 3825
PsrI GAACNNNNNNTAC 4 cut(s) 2662, 2694, 3688, 3720
PstI CTGCAG 2 cut(s) 92, 478
PstNI CAGNNNCTG 1 cut(s) 2297
PsuI RGATCY 8 cut(s) 535, 760, 1893, 2004, 2447, 2697, 3364, 4490
RseI CAYNNNNRTG 6 cut(s) 392, 611, 3219, 3615, 4872, 4953
SacI GAGCTC 3 cut(s) 429, 4098, 4287
Sau96I GGNCC 8 cut(s) 13, 602, 1223, 1224, 1844, 3178, 3763, 4074
SbfI CCTGCAGG 1 cut(s) 478
ScaI AGTACT 2 cut(s) 1956, 3995
SchI GAGTC 3 cut(s) 1035, 3694, 4507
ScrFI CCNGG 5 cut(s) 156, 977, 1192, 2171, 3161
SdaI CCTGCAGG 1 cut(s) 478
SfcI CTRYAG 5 cut(s) 88, 474, 1253, 2019, 2136
SfiI GGCCNNNNNGGCC 2 cut(s) 11, 3893
SfoI GGCGCC 1 cut(s) 253
Sfr274I CTCGAG 2 cut(s) 218, 3825
SfuI TTCGAA 1 cut(s) 4800
SinI GGWCC 2 cut(s) 3178, 4074
SlaI CTCGAG 2 cut(s) 218, 3825
SmiMI CAYNNNNRTG 6 cut(s) 392, 611, 3219, 3615, 4872, 4953
SmlI CTYRAG 5 cut(s) 218, 676, 1036, 1773, 3825
SmoI CTYRAG 5 cut(s) 218, 676, 1036, 1773, 3825
SpeI ACTAGT 1 cut(s) 4003
SphI GCATGC 3 cut(s) 3210, 3413, 4989
Sse8387I CCTGCAGG 1 cut(s) 478
SseBI AGGCCT 2 cut(s) 2076, 2914
SspDI GGCGCC 1 cut(s) 251
SspI AATATT 3 cut(s) 985, 2593, 2764
SstI GAGCTC 3 cut(s) 429, 4098, 4287
StuI AGGCCT 2 cut(s) 2076, 2914
StyD4I CCNGG 5 cut(s) 154, 975, 1190, 2169, 3159
StyI CCWWGG 3 cut(s) 306, 2685, 3414
TaqII GACCGA 1 cut(s) 3361
TatI WGTACW 6 cut(s) 704, 1149, 1812, 1954, 1975, 3993
TauI GCSGC 4 cut(s) 96, 99, 1712, 3935
TfiI GAWTC 9 cut(s) 616, 1157, 1212, 1638, 1933, 2252, 2970, 3845, 4485
TscAI CASTG 9 cut(s) 876, 943, 1071, 1390, 1488, 1765, 2358, 3663, 3739
TseFI GTSAC 4 cut(s) 867, 2097, 2112, 2999
Tsp45I GTSAC 4 cut(s) 867, 2097, 2112, 2999
TspGWI ACGGA 9 cut(s) 453, 582, 1073, 1205, 1498, 1906, 2131, 2963, 3281
TspRI CASTG 9 cut(s) 876, 943, 1071, 1390, 1488, 1765, 2358, 3663, 3739
Van91I CCANNNNNTGG 1 cut(s) 4105
Vha464I CTTAAG 1 cut(s) 676
VneI GTGCAC 2 cut(s) 408, 1761
VpaK11BI GGWCC 2 cut(s) 3178, 4074
VspI ATTAAT 1 cut(s) 4560
XagI CCTNNNNNAGG 1 cut(s) 3435
XbaI TCTAGA 2 cut(s) 763, 3688
XceI RCATGY 5 cut(s) 705, 1496, 3210, 3413, 4989
XcmI CCANNNNNNNNNTGG 2 cut(s) 2177, 4166
XhoI CTCGAG 2 cut(s) 218, 3825
XmiI GTMKAC 2 cut(s) 1059, 2793
XmnI GAANNNNTTC 1 cut(s) 2256
ZrmI AGTACT 2 cut(s) 1956, 3995
Zsp2I ATGCAT 5 cut(s) 703, 3359, 4691, 4987, 4991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.