Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0004785

Physical Location & Seq

Genomic Coordinates Forward (+)

81077903 .. 81085778

Gene Structure 7876 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022119

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2919 bp

ATGGCCTCCGTGGCCCACCGCGCCAACCCACTCGTCCACCACCTCCCTCATTTCCTCACATGGCGATCCCTACGCTTCCGCACCATCTGCAGCGGCCGCATGGGCTTCTCCTCCCACTCTCAACCCGAACCCGAACCCGAAACCCGTACTTTCCCCGGAACCAAGCTTCTCGAAACCTTCACAGAACAATTCGAGATCGGATCGCGCCTCATCACACTCGAGACCGGAAAGATCGCACGTTTCGCTAACGGCGCCGTCGTGTTGGGCATGGACGAGACCAGAGTTCTCTCCACCGTCTGCTCCGCCAAGGGTGACGCCGTTAAAGACTTTTTACCCCTCACCGTTGATTATCAAGAGAAGCAATTTGCGCAAGGTATTATACCAAACACATTCATGAGAAGGGAGGGTGCACCGAAAGAGCGTGAGCTCTTGTGTGGTCGTTTGATTGATCGGCCTATTCGTCCGTTGTTTCCTGCAGGATTTTTTCATGAGGTTCAGGTGATGGCGAGTGTTCTTTCTTCTGATGGAAAGCAAGATCCAGATGTAATGGCAGCTAATGCTACATCTGCTGCTCTTATGTTATCAGACATTCCATGGGCAGGCCCCATTGGAATGATTCGCATTGGGAGGATCGAGGGGAAGTTTATTGTTAACCCAACTATGGATGAGCTTAGCTTAAGTGATCTCAACTTAGTGTATGCATGTACAAGGGACAAAACGTTAATGATTGATGTGCAAGCTCGTGAGATTTCAGAAAAAGATCTAGAAGCTGGGATGAGATTGGCTCATCCTGAAGCAGTTAAATATATTGAACCTCAAATCAGACTGGCTGCTAAAGCTGGCAAGCATAAGAAGGAATATAAATTGTCACTGATGCCGGAGAGAACATTGGAAAAAGTCGCCCAATTGGCAGAAGCTCCCATAGAAGAAGTTTTCACTGATCCTAAGTATGGAAAGTTTGAACGTGGAGAAGCCCTGGAGAATATTGCGCAAGATGTGAGAAAAGCACTTGAAGAAGAATGTGATGAAGAAAGCTTGAGACTCTTACCGAAAGCAGTAGACACAGTGAGGAAAAAGGTTGTCCGTAGAAGAATAATTGCGGAAGGATCAAGACTTGATGGGAGGCAACTGGATGAAGTTAGGCCTGTGTACTGTGAATCTGGTAATTTGCCTATGTTGCATGGATCATCACTTTTTTCCCGTGGAGACACGCAGGTGCTCTGTACAGTAACACTTGGGGCACCTGGTGATGCACAACGCTTGGAATCCGTGGTGGGCCCCCCATCAAAGCGCTTCATGCTTCACTACAGTTTCCCCCCATTCTGTATAAATGAGGTCGGTAAACATGGTGGCTTGAACAGGCGTGAAGTTGGTCATGGAACTCTTGCGGAAAAAGCTCTGCTTGCTGTGTTACCTCCTGAAGATGACTTTCCATACACTGTTCGTATCAATTCTGAAGTAATGGCTTCTGATGGTTCAACATCTATGGCTTCTGTCTGTGGAGGCAGTATGGCTTTGATGGATGCTGGCATTCCACTGTGCAAACATGTAGCTGGTGTCTCCGTAGGTCTTGTTAGTGAAGTTGATCAATCAACTGGCACAATAAAGGAATACCGTATATTGACTGACATTCTGGGTCTGGAAGATCATTTGGGGGACATGGACTTCAAAATTGTTGGCACGCAAGATGGAATCACCGCAATTCAGTTGGATATAAAACCTGCTGGAATTCCTTTAGATATTGTTTGTGAAAGCTTGGAGGCGGCACGTAAAGGTCGTATCGAAATTCTTGATCACATGGAGAGAGAGATCAGTGCACCACGCACTCAAGATGATAGAAATTCTCCTCGATTAGCTACGTTGAAGTACAGCAATGATGCACTTCGTCGCTTGCTTGGGCCTCTGGGTGCTCTTAAAAGAAAAATAGAAGACGAAACAGGTACACGGATCTCTGTAAGTGATGGAACACTCACCATAGTTGCTAAGAATCAATCTGCAATGGACAAAGTACTCGACAAGGTTGATTTTGTACTTGGACGTGAAATTGAAATTGGTGGGATCTATAAAGGGACTGTAGCTTCAATCAAAGAATATGGTGCATTCGTGGAGTTTAATGGAGGGCAACAAGGCCTTCTACACATTTCCGAGTTGTCACATGAACCGGTGTCACGGATTTCAGATGTAGTATCTGTAGGCCAGCAGCTCTCTTTGATGTGCATAGGCCAGGATGTACGTGGTAACATCAAGTTGTCCCTGAAAGCTACATTACCTCGACCACAATCTCAGACAAAGAATTTGGCTGTGGAATCTGTTCCATCTATGAAACAAGCTCCTAGTGTTTGGGCAGCTGCTGGAGATGTGTCTAGTGGTGAAGAAAAGCATAGCCCTTTGTCTGAGGAATTGCCAGTGAGTAAATATGAAGTTAATGCAATGAAATCCTCTGCTTCCTCTAGTCCACCAGTTCTAGTCAGAAGTGCTGAAGAGTGCGACGAGGAGGAAAGATCTAATGGTTTTGTTCAAAATTCTAAAACTACTTCTGAACCCATTGGTGGTTTGAAACCAGAGCGCAAGACTGTTCAAACTACTCCTAAGACATCAAAATCTTTTTTACAAAAGGAACGAGATAAAGATGAGAAGGAACACAATATTGCATCCCAAAACCACTCTAGTAATGATTTGAGTAACAAGGAAGACCCTGTAACTGCAAAAAGTCTGAAGCTAGGAACAAAGGTTACTGCCAAGGTTTATCAGATCCGCACAGGTGGGCTGGTGCTTGATCTGGGTGGGGGACTTCGAGGAATGTATCGGTTTGACGCCAATGCTAAGAAGGACTTTGATGTCAATGATGAGTTGCGAGTTGAGTGTGTTAGTTTTTCTAGCAAGGGGATTCCGGTAATGTCTTTGGTGGATGATGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000175 GO:0000957 GO:0000959 GO:0000963 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0004654 GO:0006139 GO:0006396 GO:0006401 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008408 GO:0009056 GO:0009057 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019439 GO:0034641 GO:0034655 GO:0043170 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0046483 GO:0046700 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140053 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko03018 map00230 map00240 map03018

Pfam Domains

Protein Families

KH_1 PNPase RNase_PH RNase_PH_C S1

Protein Analysis

973

Amino Acids

106.54

Weight (kDa)

5.92

Isoelectric Point (pI)

42.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNase_PH	PF01138	68 - 198	5.9e-25	3' exoribonuclease family, domain 1
RNase_PH_C	PF03725	201 - 263	1.4e-11	3' exoribonuclease family, domain 2
PNPase	PF03726	297 - 376	1.5e-16	Polyribonucleotide nucleotidyltransferase, RNA binding domain
RNase_PH	PF01138	379 - 512	7.8e-28	3' exoribonuclease family, domain 1
S1	PF00575	682 - 753	1.3e-11	S1 RNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004785)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14580
fragaria_vesca	FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050 FvH4_6g51050
malus_domestica	MD17G1031200.v1.1
prunus_persica	Prupe.3G288400_v2.0.a1
pyrus_communis	pycom17g02860
rosa_chinensis	RchiOBHm_Chr2g0172601
rosa_laevigata	RLG00000016016 RLG00000022119
rosa_multiflora	Rmu_sc0000912.1_g000001
rosa_roxburghii	Rroxscaffold_2G00079710
rosa_rugosa	Rorug01G0489200 Rorug02G0560200
rosa_samantha	Rh2AG636600 Rh2BG650000 Rh2CG616700 Rh2DG044700 Rh2DG664200
rosa_wichuraiana	Rw2G003770 Rw2G052650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1204
AasI	GACNNNNNNGTC	1 cut(s)	2837
Acc16I	TGCGCA	2 cut(s)	369, 990
Acc36I	ACCTGC	2 cut(s)	1204, 1729
AccB1I	GGYRCC	2 cut(s)	251, 1240
AccI	GTMKAC	1 cut(s)	1059
AccII	CGCG	2 cut(s)	21, 205
AclI	AACGTT	1 cut(s)	719
AcoI	YGGCCR	1 cut(s)	94
AcsI	RAATTY	5 cut(s)	1728, 1785, 1840, 2293, 2521
AcuI	CTGAAG	5 cut(s)	813, 1440, 1476, 2499, 2735
AcyI	GRCGYC	3 cut(s)	252, 315, 2814
AdeI	CACNNNGTG	1 cut(s)	1247
AfaI	GTAC	9 cut(s)	148, 706, 1151, 1225, 1868, 1942, 2010, 2031, 2232
AfeI	AGCGCT	1 cut(s)	1292
AfiI	CCNNNNNNNGG	4 cut(s)	599, 661, 950, 2549
AflII	CTTAAG	1 cut(s)	676
AflIII	ACRYGT	1 cut(s)	1546
AgeI	ACCGGT	1 cut(s)	2161
AjiI	CACGTC	1 cut(s)	2039
AjnI	CCWGG	3 cut(s)	975, 1243, 2223
AjuI	GAANNNNNNNTTGG	2 cut(s)	2034, 2066
AleI	CACNNNNGTG	1 cut(s)	1214
Alw21I	GWGCWC	5 cut(s)	412, 429, 1221, 1819, 1912
Alw26I	GTCTC	5 cut(s)	215, 269, 1033, 1200, 1564
Alw44I	GTGCAC	2 cut(s)	408, 1815
AlwNI	CAGNNNCTG	1 cut(s)	2351
Ama87I	CYCGRG	1 cut(s)	218
Aor51HI	AGCGCT	1 cut(s)	1292
ApaI	GGGCCC	1 cut(s)	1280
ApaLI	GTGCAC	2 cut(s)	408, 1815
ApeKI	GCWGC	7 cut(s)	90, 551, 569, 830, 2200, 2345, 2348
ApoI	RAATTY	5 cut(s)	1728, 1785, 1840, 2293, 2521
ArsI	GACNNNNNNTTYG	2 cut(s)	2278, 2310
AsiGI	ACCGGT	1 cut(s)	2161
Asp700I	GAANNNNTTC	1 cut(s)	2310
AspLEI	GCGC	7 cut(s)	23, 207, 254, 370, 991, 1293, 2568
AspS9I	GGNCC	5 cut(s)	13, 602, 1276, 1277, 1898
AsuC2I	CCSGG	1 cut(s)	156
AsuHPI	GGTGA	7 cut(s)	323, 331, 511, 1259, 1687, 1963, 2381
AvaI	CYCGRG	1 cut(s)	218
BaeGI	GKGCMC	4 cut(s)	412, 1243, 1280, 1819
BanI	GGYRCC	2 cut(s)	251, 1240
BanII	GRGCYC	2 cut(s)	429, 1280
BauI	CACGAG	1 cut(s)	741
BbsI	GAAGAC	2 cut(s)	1935, 2697
Bbv12I	GWGCWC	5 cut(s)	412, 429, 1221, 1819, 1912
BbvI	GCAGC	7 cut(s)	102, 556, 563, 817, 2212, 2335, 2357
BceAI	ACGGC	3 cut(s)	239, 265, 302
BciT130I	CCWGG	3 cut(s)	977, 1245, 2225
BclI	TGATCA	2 cut(s)	1585, 1792
BcnI	CCSGG	1 cut(s)	156
BcoDI	GTCTC	5 cut(s)	215, 269, 1033, 1200, 1564
BfaI	CTAG	8 cut(s)	764, 2334, 2364, 2451, 2465, 2667, 2720, 2877
BfmI	CTRYAG	5 cut(s)	88, 474, 1306, 2073, 2190
BfoI	RGCGCY	2 cut(s)	255, 1294
BfrI	CTTAAG	1 cut(s)	676
BfuAI	ACCTGC	2 cut(s)	1204, 1729
BglI	GCCNNNNNGGC	3 cut(s)	11, 102, 908
BglII	AGATCT	2 cut(s)	760, 2501
BlpI	GCTNAGC	1 cut(s)	671
BmcAI	AGTACT	1 cut(s)	2010
Bme1390I	CCNGG	4 cut(s)	156, 977, 1245, 2225
BmeT110I	CYCGRG	1 cut(s)	218
BmgBI	CACGTC	1 cut(s)	2039
BmgT120I	GGNCC	5 cut(s)	13, 602, 1276, 1277, 1898
BmiI	GGNNCC	6 cut(s)	160, 253, 604, 1242, 1278, 1279
BmrFI	CCNGG	4 cut(s)	156, 977, 1245, 2225
BmsI	GCATC	5 cut(s)	864, 1240, 1513, 1867, 2660
BpiI	GAAGAC	2 cut(s)	1935, 2697
BplI	GAGNNNNNCTC	2 cut(s)	769, 801
BpmI	CTGGAG	2 cut(s)	998, 2373
Bpu1102I	GCTNAGC	1 cut(s)	671
BpuEI	CTTGAG	2 cut(s)	1057, 1812
BpuMI	CCSGG	1 cut(s)	156
BsaAI	YACGTR	2 cut(s)	1769, 2234
BsaHI	GRCGYC	3 cut(s)	252, 315, 2814
BsaI	GGTCTC	2 cut(s)	215, 269
BsaJI	CCNNGG	8 cut(s)	9, 154, 306, 593, 975, 1201, 1269, 2739
BsaWI	WCCGGW	3 cut(s)	224, 2161, 2890
Bsc4I	CCNNNNNNNGG	4 cut(s)	599, 661, 950, 2549
Bse118I	RCCGGY	1 cut(s)	2161
Bse1I	ACTGG	5 cut(s)	831, 1134, 1600, 2405, 2459
Bse3DI	GCAATG	3 cut(s)	1879, 2004, 2436
BseBI	CCWGG	3 cut(s)	977, 1245, 2225
BseDI	CCNNGG	8 cut(s)	9, 154, 306, 593, 975, 1201, 1269, 2739
BseGI	GGATG	8 cut(s)	670, 780, 787, 1138, 1528, 2233, 2651, 2914
BseLI	CCNNNNNNNGG	4 cut(s)	599, 661, 950, 2549
BseMI	GCAATG	3 cut(s)	1879, 2004, 2436
BseMII	CTCAG	2 cut(s)	2297, 2385
BseNI	ACTGG	5 cut(s)	831, 1134, 1600, 2405, 2459
BseRI	GAGGAG	3 cut(s)	100, 1836, 2507
BseSI	GKGCMC	4 cut(s)	412, 1243, 1280, 1819
BseX3I	CGGCCG	1 cut(s)	94
BseXI	GCAGC	7 cut(s)	102, 556, 563, 817, 2212, 2335, 2357
BseYI	CCCAGC	1 cut(s)	770
Bsh1236I	CGCG	2 cut(s)	21, 205
Bsh1285I	CGRYCG	1 cut(s)	97
BshNI	GGYRCC	2 cut(s)	251, 1240
BshTI	ACCGGT	1 cut(s)	2161
BsiEI	CGRYCG	1 cut(s)	97
BsiHKAI	GWGCWC	5 cut(s)	412, 429, 1221, 1819, 1912
BsiHKCI	CYCGRG	1 cut(s)	218
BsiSI	CCGG	5 cut(s)	156, 225, 878, 2162, 2891
BslFI	GGGAC	5 cut(s)	725, 1670, 2083, 2236, 2802
BslI	CCNNNNNNNGG	4 cut(s)	599, 661, 950, 2549
BsmAI	GTCTC	5 cut(s)	215, 269, 1033, 1200, 1564
BsmFI	GGGAC	5 cut(s)	725, 1670, 2083, 2236, 2802
BsmI	GAATGC	2 cut(s)	1530, 2099
Bso31I	GGTCTC	2 cut(s)	215, 269
BsoBI	CYCGRG	1 cut(s)	218
Bsp120I	GGGCCC	1 cut(s)	1276
Bsp1286I	GDGCHC	7 cut(s)	412, 429, 1221, 1243, 1280, 1819, 1912
Bsp1407I	TGTACA	2 cut(s)	704, 1223
Bsp1720I	GCTNAGC	1 cut(s)	671
Bsp19I	CCATGG	1 cut(s)	593
BspCNI	CTCAG	2 cut(s)	2296, 2386
BspFNI	CGCG	2 cut(s)	21, 205
BspHI	TCATGA	2 cut(s)	393, 487
BspLI	GGNNCC	6 cut(s)	160, 253, 604, 1242, 1278, 1279
BspMAI	CTGCAG	2 cut(s)	92, 478
BspMI	ACCTGC	2 cut(s)	1204, 1729
BspT107I	GGYRCC	2 cut(s)	251, 1240
BspTI	CTTAAG	1 cut(s)	676
BspTNI	GGTCTC	2 cut(s)	215, 269
BsrDI	GCAATG	3 cut(s)	1879, 2004, 2436
BsrFI	RCCGGY	1 cut(s)	2161
BsrGI	TGTACA	2 cut(s)	704, 1223
BsrI	ACTGG	5 cut(s)	831, 1134, 1600, 2405, 2459
BssAI	RCCGGY	1 cut(s)	2161
BssECI	CCNNGG	8 cut(s)	9, 154, 306, 593, 975, 1201, 1269, 2739
BssNI	GRCGYC	3 cut(s)	252, 315, 2814
BssSI	CACGAG	1 cut(s)	741
BssT1I	CCWWGG	3 cut(s)	306, 593, 2739
Bst2BI	CACGAG	1 cut(s)	741
Bst2UI	CCWGG	3 cut(s)	977, 1245, 2225
Bst6I	CTCTTC	1 cut(s)	2475
BstACI	GRCGYC	3 cut(s)	252, 315, 2814
BstAFI	CTTAAG	1 cut(s)	676
BstAPI	GCANNNNNTGC	2 cut(s)	87, 557
BstAUI	TGTACA	2 cut(s)	704, 1223
BstBAI	YACGTR	2 cut(s)	1769, 2234
BstC8I	GCNNGC	9 cut(s)	601, 738, 841, 845, 1404, 1528, 1682, 1892, 2198
BstDEI	CTNAG	8 cut(s)	671, 691, 945, 1983, 2283, 2394, 2589, 2823
BstDSI	CCRYGG	4 cut(s)	9, 593, 1201, 1269
BstF5I	GGATG	8 cut(s)	670, 780, 787, 1138, 1528, 2233, 2651, 2914
BstFNI	CGCG	2 cut(s)	21, 205
BstH2I	RGCGCY	2 cut(s)	255, 1294
BstHHI	GCGC	7 cut(s)	23, 207, 254, 370, 991, 1293, 2568
BstMAI	GTCTC	5 cut(s)	215, 269, 1033, 1200, 1564
BstMCI	CGRYCG	1 cut(s)	97
BstNI	CCWGG	3 cut(s)	977, 1245, 2225
BstNSI	RCATGY	2 cut(s)	705, 1550
BstSCI	CCNGG	4 cut(s)	154, 975, 1243, 2223
BstSFI	CTRYAG	5 cut(s)	88, 474, 1306, 2073, 2190
BstSLI	GKGCMC	4 cut(s)	412, 1243, 1280, 1819
BstUI	CGCG	2 cut(s)	21, 205
BstV1I	GCAGC	7 cut(s)	102, 556, 563, 817, 2212, 2335, 2357
BstV2I	GAAGAC	2 cut(s)	1935, 2697
BstX2I	RGATCY	6 cut(s)	535, 760, 1947, 2058, 2501, 2751
BstYI	RGATCY	6 cut(s)	535, 760, 1947, 2058, 2501, 2751
BstZI	CGGCCG	1 cut(s)	94
BtgI	CCRYGG	4 cut(s)	9, 593, 1201, 1269
BtrI	CACGTC	1 cut(s)	2039
BtsCI	GGATG	8 cut(s)	670, 780, 787, 1138, 1528, 2233, 2651, 2914
BtsIMutI	CAGTG	7 cut(s)	869, 936, 1071, 1437, 1535, 1819, 2412
BveI	ACCTGC	2 cut(s)	1204, 1729
Cac8I	GCNNGC	9 cut(s)	601, 738, 841, 845, 1404, 1528, 1682, 1892, 2198
CaiI	CAGNNNCTG	1 cut(s)	2351
CciI	TCATGA	2 cut(s)	393, 487
CciNI	GCGGCCGC	1 cut(s)	94
CfoI	GCGC	7 cut(s)	23, 207, 254, 370, 991, 1293, 2568
Cfr10I	RCCGGY	1 cut(s)	2161
Cfr13I	GGNCC	5 cut(s)	13, 602, 1276, 1277, 1898
CseI	GACGC	2 cut(s)	323, 2822
CsiI	ACCWGGT	1 cut(s)	1243
Csp6I	GTAC	9 cut(s)	147, 705, 1150, 1224, 1867, 1941, 2009, 2030, 2231
CspAI	ACCGGT	1 cut(s)	2161
CviQI	GTAC	9 cut(s)	147, 705, 1150, 1224, 1867, 1941, 2009, 2030, 2231
DdeI	CTNAG	8 cut(s)	671, 691, 945, 1983, 2283, 2394, 2589, 2823
DinI	GGCGCC	1 cut(s)	253
DraIII	CACNNNGTG	1 cut(s)	1247
DrdI	GACNNNNNNGTC	1 cut(s)	2837
DseDI	GACNNNNNNGTC	1 cut(s)	2837
EaeI	YGGCCR	1 cut(s)	94
EagI	CGGCCG	1 cut(s)	94
Eam1104I	CTCTTC	1 cut(s)	2475
EarI	CTCTTC	1 cut(s)	2475
EciI	GGCGGA	1 cut(s)	292
Ecl136II	GAGCTC	1 cut(s)	427
EclXI	CGGCCG	1 cut(s)	94
Eco130I	CCWWGG	3 cut(s)	306, 593, 2739
Eco147I	AGGCCT	2 cut(s)	1144, 2130
Eco24I	GRGCYC	2 cut(s)	429, 1280
Eco31I	GGTCTC	2 cut(s)	215, 269
Eco47III	AGCGCT	1 cut(s)	1292
Eco52I	CGGCCG	1 cut(s)	94
Eco53kI	GAGCTC	1 cut(s)	427
Eco57I	CTGAAG	5 cut(s)	813, 1440, 1476, 2499, 2735
Eco88I	CYCGRG	1 cut(s)	218
EcoICRI	GAGCTC	1 cut(s)	427
EcoO109I	RGGNCCY	2 cut(s)	602, 1277
EcoRI	GAATTC	1 cut(s)	1728
EcoRII	CCWGG	3 cut(s)	975, 1243, 2223
EcoT14I	CCWWGG	3 cut(s)	306, 593, 2739
EcoT22I	ATGCAT	1 cut(s)	703
EcoT38I	GRGCYC	2 cut(s)	429, 1280
EgeI	GGCGCC	1 cut(s)	253
EheI	GGCGCC	1 cut(s)	253
ErhI	CCWWGG	3 cut(s)	306, 593, 2739
FalI	AAGNNNNNCTT	4 cut(s)	1386, 1418, 2816, 2848
FaqI	GGGAC	5 cut(s)	725, 1670, 2083, 2236, 2802
FbaI	TGATCA	2 cut(s)	1585, 1792
FblI	GTMKAC	1 cut(s)	1059
FokI	GGATG	7 cut(s)	677, 774, 787, 1145, 1535, 2240, 2638
FriOI	GRGCYC	2 cut(s)	429, 1280
FspBI	CTAG	8 cut(s)	764, 2334, 2364, 2451, 2465, 2667, 2720, 2877
FspI	TGCGCA	2 cut(s)	369, 990
GlaI	GCGC	7 cut(s)	22, 206, 253, 369, 990, 1292, 2567
GsaI	CCCAGC	1 cut(s)	774
GsuI	CTGGAG	2 cut(s)	998, 2373
HaeII	RGCGCY	2 cut(s)	255, 1294
HapII	CCGG	5 cut(s)	156, 225, 878, 2162, 2891
HgaI	GACGC	2 cut(s)	323, 2822
HhaI	GCGC	7 cut(s)	23, 207, 254, 370, 991, 1293, 2568
Hin1I	GRCGYC	3 cut(s)	252, 315, 2814
Hin6I	GCGC	7 cut(s)	21, 205, 252, 368, 989, 1291, 2566
HinP1I	GCGC	7 cut(s)	21, 205, 252, 368, 989, 1291, 2566
HincII	GTYRAC	1 cut(s)	652
HindII	GTYRAC	1 cut(s)	652
HindIII	AAGCTT	3 cut(s)	164, 1033, 1753
HinfI	GANTC	8 cut(s)	616, 1041, 1157, 1265, 1692, 1987, 2306, 2887
HpaI	GTTAAC	1 cut(s)	652
HpaII	CCGG	5 cut(s)	156, 225, 878, 2162, 2891
HphI	GGTGA	7 cut(s)	323, 331, 511, 1259, 1687, 1963, 2381
Hpy166II	GTNNAC	9 cut(s)	37, 410, 652, 1060, 1150, 1343, 1817, 1943, 2456
Hpy8I	GTNNAC	9 cut(s)	37, 410, 652, 1060, 1150, 1343, 1817, 1943, 2456
Hpy99I	CGWCG	3 cut(s)	260, 1890, 2492
HpyAV	CCTTC	7 cut(s)	187, 393, 847, 1097, 2141, 2629, 2821
HpyCH4IV	ACGT	7 cut(s)	238, 719, 964, 1768, 1859, 2038, 2233
HpyF3I	CTNAG	8 cut(s)	671, 691, 945, 1983, 2283, 2394, 2589, 2823
HpySE526I	ACGT	7 cut(s)	238, 719, 964, 1768, 1859, 2038, 2233
Hsp92I	GRCGYC	3 cut(s)	252, 315, 2814
HspAI	GCGC	7 cut(s)	21, 205, 252, 368, 989, 1291, 2566
KasI	GGCGCC	1 cut(s)	251
Ksp22I	TGATCA	2 cut(s)	1585, 1792
KspAI	GTTAAC	1 cut(s)	652
LmnI	GCTCC	3 cut(s)	305, 922, 2335
Lsp1109I	GCAGC	7 cut(s)	102, 556, 563, 817, 2212, 2335, 2357
LweI	GCATC	5 cut(s)	864, 1240, 1513, 1867, 2660
MabI	ACCWGGT	1 cut(s)	1243
MaeI	CTAG	8 cut(s)	764, 2334, 2364, 2451, 2465, 2667, 2720, 2877
MaeII	ACGT	7 cut(s)	238, 719, 964, 1768, 1859, 2038, 2233
MfeI	CAATTG	1 cut(s)	905
MflI	RGATCY	6 cut(s)	535, 760, 1947, 2058, 2501, 2751
MhlI	GDGCHC	7 cut(s)	412, 429, 1221, 1243, 1280, 1819, 1912
Mly113I	GGCGCC	1 cut(s)	252
MlyI	GAGTC	1 cut(s)	1035
MmeI	TCCRAC	1 cut(s)	1689
Mph1103I	ATGCAT	1 cut(s)	703
MroXI	GAANNNNTTC	1 cut(s)	2310
MseI	TTAA	8 cut(s)	321, 651, 677, 722, 801, 1914, 2112, 2424
MslI	CAYNNNNRTG	3 cut(s)	392, 611, 1214
MspA1I	CMGCKG	2 cut(s)	93, 2348
MspCI	CTTAAG	1 cut(s)	676
MspI	CCGG	5 cut(s)	156, 225, 878, 2162, 2891
MspR9I	CCNGG	4 cut(s)	156, 977, 1245, 2225
MunI	CAATTG	1 cut(s)	905
Mva1269I	GAATGC	2 cut(s)	1530, 2099
MvaI	CCWGG	3 cut(s)	977, 1245, 2225
MvnI	CGCG	2 cut(s)	21, 205
NarI	GGCGCC	1 cut(s)	252
NciI	CCSGG	1 cut(s)	156
NcoI	CCATGG	1 cut(s)	593
NlaIV	GGNNCC	6 cut(s)	160, 253, 604, 1242, 1278, 1279
NmuCI	GTSAC	4 cut(s)	311, 867, 2151, 2166
NotI	GCGGCCGC	1 cut(s)	94
NsbI	TGCGCA	2 cut(s)	369, 990
NsiI	ATGCAT	1 cut(s)	703
NspI	RCATGY	2 cut(s)	705, 1550
OliI	CACNNNNGTG	1 cut(s)	1214
PaeR7I	CTCGAG	1 cut(s)	218
PagI	TCATGA	2 cut(s)	393, 487
PaqCI	CACCTGC	1 cut(s)	1204
PceI	AGGCCT	2 cut(s)	1144, 2130
PciI	ACATGT	1 cut(s)	1546
PcsI	WCGNNNNNNNCGW	3 cut(s)	255, 457, 1774
PctI	GAATGC	2 cut(s)	1530, 2099
PdmI	GAANNNNTTC	1 cut(s)	2310
PfeI	GAWTC	7 cut(s)	616, 1157, 1265, 1692, 1987, 2306, 2887
PinAI	ACCGGT	1 cut(s)	2161
PleI	GAGTC	1 cut(s)	1035
PluTI	GGCGCC	1 cut(s)	255
PpsI	GAGTC	1 cut(s)	1035
Ppu21I	YACGTR	2 cut(s)	1769, 2234
PscI	ACATGT	1 cut(s)	1546
Psp124BI	GAGCTC	1 cut(s)	429
Psp1406I	AACGTT	1 cut(s)	719
Psp6I	CCWGG	3 cut(s)	975, 1243, 2223
PspFI	CCCAGC	1 cut(s)	770
PspGI	CCWGG	3 cut(s)	975, 1243, 2223
PspN4I	GGNNCC	6 cut(s)	160, 253, 604, 1242, 1278, 1279
PspOMI	GGGCCC	1 cut(s)	1276
PspPI	GGNCC	5 cut(s)	13, 602, 1276, 1277, 1898
PsrI	GAACNNNNNNTAC	2 cut(s)	2716, 2748
PstI	CTGCAG	2 cut(s)	92, 478
PstNI	CAGNNNCTG	1 cut(s)	2351
PsuI	RGATCY	6 cut(s)	535, 760, 1947, 2058, 2501, 2751
PvuII	CAGCTG	1 cut(s)	2348
RsaI	GTAC	9 cut(s)	148, 706, 1151, 1225, 1868, 1942, 2010, 2031, 2232
RsaNI	GTAC	9 cut(s)	147, 705, 1150, 1224, 1867, 1941, 2009, 2030, 2231
RseI	CAYNNNNRTG	3 cut(s)	392, 611, 1214
SacI	GAGCTC	1 cut(s)	429
SaqAI	TTAA	8 cut(s)	321, 651, 677, 722, 801, 1914, 2112, 2424
Sau96I	GGNCC	5 cut(s)	13, 602, 1276, 1277, 1898
SbfI	CCTGCAGG	1 cut(s)	478
ScaI	AGTACT	1 cut(s)	2010
SchI	GAGTC	1 cut(s)	1035
ScrFI	CCNGG	4 cut(s)	156, 977, 1245, 2225
SdaI	CCTGCAGG	1 cut(s)	478
SduI	GDGCHC	7 cut(s)	412, 429, 1221, 1243, 1280, 1819, 1912
SexAI	ACCWGGT	1 cut(s)	1243
SfaNI	GCATC	5 cut(s)	864, 1240, 1513, 1867, 2660
SfcI	CTRYAG	5 cut(s)	88, 474, 1306, 2073, 2190
SfiI	GGCCNNNNNGGCC	1 cut(s)	11
SfoI	GGCGCC	1 cut(s)	253
Sfr274I	CTCGAG	1 cut(s)	218
SlaI	CTCGAG	1 cut(s)	218
SmiMI	CAYNNNNRTG	3 cut(s)	392, 611, 1214
SmlI	CTYRAG	4 cut(s)	218, 676, 1036, 1827
SmoI	CTYRAG	4 cut(s)	218, 676, 1036, 1827
Sse8387I	CCTGCAGG	1 cut(s)	478
SseBI	AGGCCT	2 cut(s)	1144, 2130
SspDI	GGCGCC	1 cut(s)	251
SspI	AATATT	2 cut(s)	985, 2647
SspMI	CTAG	8 cut(s)	764, 2334, 2364, 2451, 2465, 2667, 2720, 2877
SstI	GAGCTC	1 cut(s)	429
StuI	AGGCCT	2 cut(s)	1144, 2130
StyD4I	CCNGG	4 cut(s)	154, 975, 1243, 2223
StyI	CCWWGG	3 cut(s)	306, 593, 2739
TaiI	ACGT	7 cut(s)	241, 722, 967, 1771, 1862, 2041, 2236
TaqI	TCGA	9 cut(s)	171, 192, 219, 633, 1782, 1849, 2013, 2272, 2794
TatI	WGTACW	6 cut(s)	704, 1149, 1223, 1866, 2008, 2029
TauI	GCSGC	3 cut(s)	96, 99, 1766
TfiI	GAWTC	7 cut(s)	616, 1157, 1265, 1692, 1987, 2306, 2887
Tru1I	TTAA	8 cut(s)	321, 651, 677, 722, 801, 1914, 2112, 2424
Tru9I	TTAA	8 cut(s)	321, 651, 677, 722, 801, 1914, 2112, 2424
TscAI	CASTG	7 cut(s)	876, 943, 1071, 1444, 1542, 1819, 2412
TseFI	GTSAC	4 cut(s)	311, 867, 2151, 2166
TseI	GCWGC	7 cut(s)	90, 551, 569, 830, 2200, 2345, 2348
Tsp45I	GTSAC	4 cut(s)	311, 867, 2151, 2166
TspDTI	ATGAA	9 cut(s)	382, 476, 1041, 1149, 1285, 2172, 2336, 2433, 2447
TspGWI	ACGGA	6 cut(s)	453, 1073, 1258, 1552, 1960, 2185
TspRI	CASTG	7 cut(s)	876, 943, 1071, 1444, 1542, 1819, 2412
Vha464I	CTTAAG	1 cut(s)	676
VneI	GTGCAC	2 cut(s)	408, 1815
XapI	RAATTY	5 cut(s)	1728, 1785, 1840, 2293, 2521
XbaI	TCTAGA	1 cut(s)	763
XceI	RCATGY	2 cut(s)	705, 1550
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2231
XhoI	CTCGAG	1 cut(s)	218
XmiI	GTMKAC	1 cut(s)	1059
XmnI	GAANNNNTTC	1 cut(s)	2310
XspI	CTAG	8 cut(s)	764, 2334, 2364, 2451, 2465, 2667, 2720, 2877
ZrmI	AGTACT	1 cut(s)	2010
Zsp2I	ATGCAT	1 cut(s)	703

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.