RchiOBHm_Chr2g0172601

Polyribonucleotide nucleotidyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004785
Physical Location & Seq
Forward (+)
85897746 .. 85905987
8242 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53988

Sequence Viewer

Length: 2919 bp
ATGGCCTCCGTGGCCCACCGCGGCAACCCACTCCTCCACCACCTCCCTCATTTCCTCACATGGCGATCCCTACGCTTCCGCACCATCTGCAGCGGCCGCATGGGCTTCTCGTCCCACTCTCAACCCGAACCCGAACCCGAAACCCGTACTCTCCCCGGAACCAAGCTCCTCGAAACCTTCACAGAACAATTCGAGATCGGATCGCGCCTCATCACACTCGAAACCGGAAAGATCGCACGTTTCGCTAACGGCGCCGTCGTTTTGGGCATGGACGAGACCAGAGTTCTCTCCACCGTCTGCTCCGCCAAGGGTGACGCCGTTAAAGACTTTTTACCCCTTACCGTTGATTATCAAGAGAAGCAATTTGCACAAGGTATTATACCAAACACATTCATGAGAAGGGAGGGCGCACCGAAAGAGCGTGAGCTCTTGTGTGGTCGTTTGATTGATCGGCCAATTCGTCCTTTGTTCCCTGCAGGATTTTTCCATGAGGTTCAGGTGATGGCGAGTGTTCTTTCTTCTGATGGAAAGCAAGATCCAGATGTAATGGCAGCTAATGCTACGTCTGCTGCTCTTATGTTATCAGACATTCCGTGGGCAGGCCCCATTGGAATGATTCGCATTGGGAGGATCGAGGGGAAGTTTATTGTTAACCCAACTATGGATGAGCTTAGCTTAAGTGATCTCAACTTAGTGTATGCATGTACAAGGGACAAAACGTTAATGATTGATGTGCAAGCTCGTGAGATTTCAGAAAAAGATCTAGAAGCTGGGATGAGATTGGCTCATCCTGAAGCAGTTAAATATCTTGAACCTCAAATCAGACTGGCTGCTAAAGCTGGCAAGCATAAGAAGGAATATAAATTGTCACTGATGCCGGAGAGAACATTGGAGAAAGTCGCTCAATTGGCAGAAGCTCCCATAGAAGAAGTTTTCACTGATCCCAAGTATGGAAAGTTTGAACGTGGAGAAGCCCTGGAGAATATTGCGCAAGATGTGAGAAAAGCACTTGAAGAAGAGTGTGATGAAGAAAGCTTGAGACTTTTACCGAAAGCAGTAGACACAGTGAGGAAAAAGGTTGTCCGAAGAAGAATAATTGCGGAAGGATCAAGACTTGATGGGAGGCGACTGGATGAAGTTAGGCCCGTGTACTGTGAATCTGGTAATTTGCCTATGTTGCATGGGTCATCACTTTTTTCTCGTGGAGACACGCAGGTACTCTGTACCGTAACACTTGGGGCACCTGGCGATGCACAACGCTTGGAATCTGTGGTGGGCCCCCCATCAAAGCGCTTCATGCTTCACTACAGTTTCCCGCCATTCTGTATAAATGAGGTCGGTAAACATGGTGGCTTGAACAGGCGTGAAGTTGGTCATGGAACTTTGGCGGAAAAAGCTCTGCTTGCCGTGTTACCTCCTGAAGATGACTTTCCATACACTGTTCGTATCAATTCTGAAGTAATGGCTTCTGATGGTTCAACATCTATGGCTTCTGTCTGTGGAGGCAGTATGGCTTTGATGGATGCTGGCATTCCACTGCGCAAACATGTAGCTGGTGTCTCCGTAGGTCTTGTTAGTGAAGTTGATCAATCAACTGGCACAATAAAGGAATACCGTATATTGACTGACATTCTGGGTCTGGAAGATCATTTGGGGGACATGGACTTCAAAATTGTTGGCACGCAAGATGGAATCACCGCAATTCAATTGGATATAAAACCTGCTGGAATTCCTTTAGATATTGTTTGTGAAAGCTTGGAGGCAGCACGTAAAGGTCGTATCCAAATTCTTGATCACATGGAGAGAGAGATCAGTGCACCTCGCACTCAAGATGATAGAAATTCTCCTCGATTAGCTACGTTGAAGTACAGCAATGATGCACTTCGTCGATTGCTTGGGCCTCTGGGTGCTCTTAAAAGAAAAATAGAAGACGAAACAGGTACACGGATCTCTGTAAGTGATGGAACACTCACCATAGTTGCTAAGAATCAATCTGCAATGGACAAAGTACTCGAAAAGGTTGATTTTGTACTTGGACGTGAAATTGAAATTGGTGGGGTCTATAAAGGGACTGTAGCTTCAATCAAAGAATATGGCGCATTTGTGGAGTTTAATGGAGGGCAACAAGGCCTTCTACACATTTCCGAGTTGTCACATGAACCGGTGTCACGGATTTCAGATGTAGTGTCTGTAGGCCAGCAGCTCTCTTTGATGTGCATAGGCCAGGATGTTCGTGGTAACATTAAGTTGTCCCTGAAAGCTACATTACCTCGACCACAATCTCAGACAAATAATTTGGCTGTGGAATCTGTTCCATCTATGAAACAAGCTCCTAGTGTTTGGGCAGCTGCTGGAGATGTGTGTAGTGGCAAAGAAAAGCATAGCCATTTGTCTGAGGAATTGCCAGTGAGTAAATATGAAGTTAATGCGATAAAATCCTCTGCTTCCTCTAGTCCACCAGTTCTAGTCAGAAGTGCTGAAGAGTGCGACGAGGAGGAAAGATCTAGTGGTTTTGTTCAAAATTCTAAAACTACTTCTGAACCCATTGGTGGTTTGAAACCAGAGTGCAAGACGGTTCAATCTACTCCTAAGACATCAAAATCTTTCTTACAAAAGGAACGAGATAAAGATGAGAAGGAACACAATATTGCATCCCAAAACCACTCTAGTAATGATTTGAGTAACAAGGAAGACCCTGTAACTGCAAAAAGTCTGAAGCTAGGAACAAAGGTTACTGCTAAGGTTTATCAGATCCGCACAGGTGGGCTGGTGCTTGATCTGGGTAGGGGACTTCGAGGAATGTATCGGTTTGAGGCCAATGCTAAGAAGGACTTTGATGTCAACGATGAGTTGCGAGTTGAGTGTGTTAGTTTTTCTAGCAAGGGGATTCCGGTAATGTCTTTGGTGGATGATGAATAG

Protein Analysis

972

Amino Acids

106.62

Weight (kDa)

6.17

Isoelectric Point (pI)

41.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_PH PF01138 68 - 198 5.9e-25 3' exoribonuclease family, domain 1
RNase_PH_C PF03725 201 - 263 1.4e-11 3' exoribonuclease family, domain 2
PNPase PF03726 297 - 376 1.9e-16 Polyribonucleotide nucleotidyltransferase, RNA binding domain
RNase_PH PF01138 379 - 512 7.8e-28 3' exoribonuclease family, domain 1
S1 PF00575 682 - 753 7.8e-12 S1 RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2837
Acc16I TGCGCA 2 cut(s) 990, 1541
Acc36I ACCTGC 2 cut(s) 1204, 1729
AccB1I GGYRCC 2 cut(s) 251, 1240
AccI GTMKAC 1 cut(s) 1059
AccII CGCG 2 cut(s) 21, 205
AclI AACGTT 1 cut(s) 719
AclWI GGATC 8 cut(s) 60, 208, 530, 638, 935, 1114, 1955, 2746
AcoI YGGCCR 2 cut(s) 94, 452
AcsI RAATTY 4 cut(s) 1728, 1785, 1840, 2521
AcuI CTGAAG 5 cut(s) 813, 1440, 1476, 2499, 2735
AcyI GRCGYC 2 cut(s) 252, 315
AfaI GTAC 9 cut(s) 148, 706, 1151, 1218, 1225, 1868, 1942, 2010, 2031
AfeI AGCGCT 1 cut(s) 1292
AfiI CCNNNNNNNGG 4 cut(s) 599, 661, 950, 2549
AflII CTTAAG 1 cut(s) 676
AflIII ACRYGT 1 cut(s) 1546
AgeI ACCGGT 1 cut(s) 2161
AjiI CACGTC 1 cut(s) 2039
AjnI CCWGG 3 cut(s) 975, 1243, 2223
AjuI GAANNNNNNNTTGG 2 cut(s) 2034, 2066
Alw21I GWGCWC 3 cut(s) 429, 1819, 1912
Alw26I GTCTC 4 cut(s) 269, 1033, 1200, 1564
Alw44I GTGCAC 1 cut(s) 1815
AlwI GGATC 8 cut(s) 60, 208, 530, 638, 935, 1114, 1955, 2746
AlwNI CAGNNNCTG 1 cut(s) 2351
Aor51HI AGCGCT 1 cut(s) 1292
ApaI GGGCCC 1 cut(s) 1280
ApaLI GTGCAC 1 cut(s) 1815
ApeKI GCWGC 8 cut(s) 90, 551, 569, 830, 1763, 2200, 2345, 2348
ApoI RAATTY 4 cut(s) 1728, 1785, 1840, 2521
ArsI GACNNNNNNTTYG 2 cut(s) 2278, 2310
AsiGI ACCGGT 1 cut(s) 2161
Asp700I GAANNNNTTC 1 cut(s) 2310
AspLEI GCGC 7 cut(s) 207, 254, 410, 991, 1293, 1542, 2099
AspS9I GGNCC 6 cut(s) 13, 602, 1143, 1276, 1277, 1898
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 4 cut(s) 323, 511, 1687, 1963
BaeGI GKGCMC 3 cut(s) 1243, 1280, 1819
BanI GGYRCC 2 cut(s) 251, 1240
BanII GRGCYC 2 cut(s) 429, 1280
BauI CACGAG 2 cut(s) 741, 1200
BbsI GAAGAC 2 cut(s) 1935, 2697
Bbv12I GWGCWC 3 cut(s) 429, 1819, 1912
BbvI GCAGC 8 cut(s) 102, 556, 563, 817, 1775, 2212, 2335, 2357
BceAI ACGGC 4 cut(s) 239, 265, 302, 1391
BciT130I CCWGG 3 cut(s) 977, 1245, 2225
BciVI GTATCC 1 cut(s) 1790
BclI TGATCA 2 cut(s) 1585, 1792
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 4 cut(s) 269, 1033, 1200, 1564
BfaI CTAG 8 cut(s) 764, 2334, 2451, 2465, 2505, 2667, 2720, 2877
BfmI CTRYAG 5 cut(s) 88, 474, 1306, 2073, 2190
BfoI RGCGCY 2 cut(s) 255, 1294
BfrI CTTAAG 1 cut(s) 676
BfuAI ACCTGC 2 cut(s) 1204, 1729
BfuI GTATCC 1 cut(s) 1790
BglI GCCNNNNNGGC 2 cut(s) 11, 102
BglII AGATCT 2 cut(s) 760, 2501
BlpI GCTNAGC 1 cut(s) 671
BmcAI AGTACT 1 cut(s) 2010
Bme1390I CCNGG 4 cut(s) 156, 977, 1245, 2225
BmgBI CACGTC 1 cut(s) 2039
BmgT120I GGNCC 6 cut(s) 13, 602, 1143, 1276, 1277, 1898
BmiI GGNNCC 6 cut(s) 160, 253, 604, 1242, 1278, 1279
BmrFI CCNGG 4 cut(s) 156, 977, 1245, 2225
BmsI GCATC 5 cut(s) 864, 1240, 1513, 1867, 2660
BpiI GAAGAC 2 cut(s) 1935, 2697
BplI GAGNNNNNCTC 2 cut(s) 769, 801
BpmI CTGGAG 2 cut(s) 998, 2373
Bpu10I CCTNAGC 1 cut(s) 2739
Bpu1102I GCTNAGC 1 cut(s) 671
BpuEI CTTGAG 2 cut(s) 1057, 1812
BpuMI CCSGG 1 cut(s) 156
BsaAI YACGTR 1 cut(s) 1769
BsaHI GRCGYC 2 cut(s) 252, 315
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 6 cut(s) 9, 19, 154, 306, 593, 975
BsaWI WCCGGW 3 cut(s) 224, 2161, 2890
Bsc4I CCNNNNNNNGG 4 cut(s) 599, 661, 950, 2549
Bse118I RCCGGY 1 cut(s) 2161
Bse1I ACTGG 5 cut(s) 831, 1134, 1600, 2405, 2459
Bse3DI GCAATG 2 cut(s) 1879, 2004
BseBI CCWGG 3 cut(s) 977, 1245, 2225
BseDI CCNNGG 6 cut(s) 9, 19, 154, 306, 593, 975
BseGI GGATG 8 cut(s) 670, 780, 787, 1138, 1528, 2233, 2651, 2914
BseLI CCNNNNNNNGG 4 cut(s) 599, 661, 950, 2549
BseMI GCAATG 2 cut(s) 1879, 2004
BseMII CTCAG 2 cut(s) 2297, 2385
BseNI ACTGG 5 cut(s) 831, 1134, 1600, 2405, 2459
BseRI GAGGAG 4 cut(s) 23, 158, 1836, 2507
BseSI GKGCMC 3 cut(s) 1243, 1280, 1819
BseX3I CGGCCG 1 cut(s) 94
BseXI GCAGC 8 cut(s) 102, 556, 563, 817, 1775, 2212, 2335, 2357
BseYI CCCAGC 1 cut(s) 770
Bsh1236I CGCG 2 cut(s) 21, 205
Bsh1285I CGRYCG 1 cut(s) 97
BshNI GGYRCC 2 cut(s) 251, 1240
BshTI ACCGGT 1 cut(s) 2161
BsiEI CGRYCG 1 cut(s) 97
BsiHKAI GWGCWC 3 cut(s) 429, 1819, 1912
BsiSI CCGG 5 cut(s) 156, 225, 878, 2162, 2891
BslFI GGGAC 6 cut(s) 97, 725, 1670, 2083, 2236, 2802
BslI CCNNNNNNNGG 4 cut(s) 599, 661, 950, 2549
BsmAI GTCTC 4 cut(s) 269, 1033, 1200, 1564
BsmFI GGGAC 6 cut(s) 97, 725, 1670, 2083, 2236, 2802
BsmI GAATGC 1 cut(s) 1530
Bso31I GGTCTC 1 cut(s) 269
Bsp120I GGGCCC 1 cut(s) 1276
Bsp1286I GDGCHC 5 cut(s) 429, 1243, 1280, 1819, 1912
Bsp1407I TGTACA 1 cut(s) 704
Bsp1720I GCTNAGC 1 cut(s) 671
BspCNI CTCAG 2 cut(s) 2296, 2386
BspFNI CGCG 2 cut(s) 21, 205
BspHI TCATGA 1 cut(s) 393
BspLI GGNNCC 6 cut(s) 160, 253, 604, 1242, 1278, 1279
BspMAI CTGCAG 2 cut(s) 92, 478
BspMI ACCTGC 2 cut(s) 1204, 1729
BspPI GGATC 8 cut(s) 60, 208, 530, 638, 935, 1114, 1955, 2746
BspT107I GGYRCC 2 cut(s) 251, 1240
BspTI CTTAAG 1 cut(s) 676
BspTNI GGTCTC 1 cut(s) 269
BsrDI GCAATG 2 cut(s) 1879, 2004
BsrFI RCCGGY 1 cut(s) 2161
BsrGI TGTACA 1 cut(s) 704
BsrI ACTGG 5 cut(s) 831, 1134, 1600, 2405, 2459
BssAI RCCGGY 1 cut(s) 2161
BssECI CCNNGG 6 cut(s) 9, 19, 154, 306, 593, 975
BssNI GRCGYC 2 cut(s) 252, 315
BssSI CACGAG 2 cut(s) 741, 1200
BssT1I CCWWGG 1 cut(s) 306
Bst2BI CACGAG 2 cut(s) 741, 1200
Bst2UI CCWGG 3 cut(s) 977, 1245, 2225
Bst6I CTCTTC 2 cut(s) 1011, 2475
BstACI GRCGYC 2 cut(s) 252, 315
BstAFI CTTAAG 1 cut(s) 676
BstAPI GCANNNNNTGC 2 cut(s) 87, 557
BstAUI TGTACA 1 cut(s) 704
BstBAI YACGTR 1 cut(s) 1769
BstC8I GCNNGC 8 cut(s) 601, 738, 841, 845, 1404, 1528, 1682, 2198
BstDEI CTNAG 8 cut(s) 671, 691, 1983, 2283, 2394, 2589, 2739, 2823
BstDSI CCRYGG 3 cut(s) 9, 19, 593
BstF5I GGATG 8 cut(s) 670, 780, 787, 1138, 1528, 2233, 2651, 2914
BstFNI CGCG 2 cut(s) 21, 205
BstH2I RGCGCY 2 cut(s) 255, 1294
BstHHI GCGC 7 cut(s) 207, 254, 410, 991, 1293, 1542, 2099
BstMAI GTCTC 4 cut(s) 269, 1033, 1200, 1564
BstMCI CGRYCG 1 cut(s) 97
BstNI CCWGG 3 cut(s) 977, 1245, 2225
BstNSI RCATGY 2 cut(s) 705, 1550
BstSCI CCNGG 4 cut(s) 154, 975, 1243, 2223
BstSFI CTRYAG 5 cut(s) 88, 474, 1306, 2073, 2190
BstSLI GKGCMC 3 cut(s) 1243, 1280, 1819
BstUI CGCG 2 cut(s) 21, 205
BstV1I GCAGC 8 cut(s) 102, 556, 563, 817, 1775, 2212, 2335, 2357
BstV2I GAAGAC 2 cut(s) 1935, 2697
BstX2I RGATCY 5 cut(s) 535, 760, 1947, 2501, 2751
BstYI RGATCY 5 cut(s) 535, 760, 1947, 2501, 2751
BstZI CGGCCG 1 cut(s) 94
BsuI GTATCC 1 cut(s) 1790
BtgI CCRYGG 3 cut(s) 9, 19, 593
BtgZI GCGATG 1 cut(s) 1263
BtrI CACGTC 1 cut(s) 2039
BtsCI GGATG 8 cut(s) 670, 780, 787, 1138, 1528, 2233, 2651, 2914
BtsI GCAGTG 1 cut(s) 1535
BtsIMutI CAGTG 7 cut(s) 869, 936, 1071, 1437, 1535, 1819, 2412
BveI ACCTGC 2 cut(s) 1204, 1729
Cac8I GCNNGC 8 cut(s) 601, 738, 841, 845, 1404, 1528, 1682, 2198
CaiI CAGNNNCTG 1 cut(s) 2351
CciI TCATGA 1 cut(s) 393
CciNI GCGGCCGC 1 cut(s) 94
CfoI GCGC 7 cut(s) 207, 254, 410, 991, 1293, 1542, 2099
Cfr10I RCCGGY 1 cut(s) 2161
Cfr13I GGNCC 6 cut(s) 13, 602, 1143, 1276, 1277, 1898
Cfr42I CCGCGG 1 cut(s) 22
CseI GACGC 1 cut(s) 323
Csp6I GTAC 9 cut(s) 147, 705, 1150, 1217, 1224, 1867, 1941, 2009, 2030
CspAI ACCGGT 1 cut(s) 2161
CviQI GTAC 9 cut(s) 147, 705, 1150, 1217, 1224, 1867, 1941, 2009, 2030
DdeI CTNAG 8 cut(s) 671, 691, 1983, 2283, 2394, 2589, 2739, 2823
DinI GGCGCC 1 cut(s) 253
DrdI GACNNNNNNGTC 1 cut(s) 2837
DseDI GACNNNNNNGTC 1 cut(s) 2837
EaeI YGGCCR 2 cut(s) 94, 452
EagI CGGCCG 1 cut(s) 94
Eam1104I CTCTTC 2 cut(s) 1011, 2475
EarI CTCTTC 2 cut(s) 1011, 2475
EciI GGCGGA 2 cut(s) 292, 1403
Ecl136II GAGCTC 1 cut(s) 427
EclXI CGGCCG 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 306
Eco147I AGGCCT 1 cut(s) 2130
Eco24I GRGCYC 2 cut(s) 429, 1280
Eco31I GGTCTC 1 cut(s) 269
Eco47III AGCGCT 1 cut(s) 1292
Eco52I CGGCCG 1 cut(s) 94
Eco53kI GAGCTC 1 cut(s) 427
Eco57I CTGAAG 5 cut(s) 813, 1440, 1476, 2499, 2735
EcoICRI GAGCTC 1 cut(s) 427
EcoO109I RGGNCCY 2 cut(s) 602, 1277
EcoRI GAATTC 1 cut(s) 1728
EcoRII CCWGG 3 cut(s) 975, 1243, 2223
EcoT14I CCWWGG 1 cut(s) 306
EcoT22I ATGCAT 1 cut(s) 703
EcoT38I GRGCYC 2 cut(s) 429, 1280
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 306
FalI AAGNNNNNCTT 4 cut(s) 1386, 1418, 2816, 2848
FaqI GGGAC 6 cut(s) 97, 725, 1670, 2083, 2236, 2802
FauI CCCGC 1 cut(s) 1323
FbaI TGATCA 2 cut(s) 1585, 1792
FblI GTMKAC 1 cut(s) 1059
FokI GGATG 7 cut(s) 677, 774, 787, 1145, 1535, 2240, 2638
FriOI GRGCYC 2 cut(s) 429, 1280
FspBI CTAG 8 cut(s) 764, 2334, 2451, 2465, 2505, 2667, 2720, 2877
FspI TGCGCA 2 cut(s) 990, 1541
GlaI GCGC 7 cut(s) 206, 253, 409, 990, 1292, 1541, 2098
GsaI CCCAGC 1 cut(s) 774
GsuI CTGGAG 2 cut(s) 998, 2373
HaeII RGCGCY 2 cut(s) 255, 1294
HapII CCGG 5 cut(s) 156, 225, 878, 2162, 2891
HgaI GACGC 1 cut(s) 323
HhaI GCGC 7 cut(s) 207, 254, 410, 991, 1293, 1542, 2099
Hin1I GRCGYC 2 cut(s) 252, 315
Hin6I GCGC 7 cut(s) 205, 252, 408, 989, 1291, 1540, 2097
HinP1I GCGC 7 cut(s) 205, 252, 408, 989, 1291, 1540, 2097
HincII GTYRAC 2 cut(s) 652, 2842
HindII GTYRAC 2 cut(s) 652, 2842
HindIII AAGCTT 2 cut(s) 1033, 1753
HinfI GANTC 7 cut(s) 616, 1157, 1265, 1692, 1987, 2306, 2887
HpaI GTTAAC 1 cut(s) 652
HpaII CCGG 5 cut(s) 156, 225, 878, 2162, 2891
HphI GGTGA 4 cut(s) 323, 511, 1687, 1963
Hpy166II GTNNAC 8 cut(s) 652, 1060, 1150, 1343, 1817, 1943, 2456, 2842
Hpy8I GTNNAC 8 cut(s) 652, 1060, 1150, 1343, 1817, 1943, 2456, 2842
Hpy99I CGWCG 3 cut(s) 260, 1890, 2492
HpyAV CCTTC 7 cut(s) 187, 393, 847, 1097, 2141, 2629, 2821
HpyCH4IV ACGT 7 cut(s) 238, 563, 719, 964, 1768, 1859, 2038
HpyF3I CTNAG 8 cut(s) 671, 691, 1983, 2283, 2394, 2589, 2739, 2823
HpySE526I ACGT 7 cut(s) 238, 563, 719, 964, 1768, 1859, 2038
Hsp92I GRCGYC 2 cut(s) 252, 315
HspAI GCGC 7 cut(s) 205, 252, 408, 989, 1291, 1540, 2097
KasI GGCGCC 1 cut(s) 251
Ksp22I TGATCA 2 cut(s) 1585, 1792
KspAI GTTAAC 1 cut(s) 652
KspI CCGCGG 1 cut(s) 22
LmnI GCTCC 4 cut(s) 171, 305, 922, 2335
Lsp1109I GCAGC 8 cut(s) 102, 556, 563, 817, 1775, 2212, 2335, 2357
LweI GCATC 5 cut(s) 864, 1240, 1513, 1867, 2660
MaeI CTAG 8 cut(s) 764, 2334, 2451, 2465, 2505, 2667, 2720, 2877
MaeII ACGT 7 cut(s) 238, 563, 719, 964, 1768, 1859, 2038
MfeI CAATTG 2 cut(s) 905, 1706
MflI RGATCY 5 cut(s) 535, 760, 1947, 2501, 2751
MhlI GDGCHC 5 cut(s) 429, 1243, 1280, 1819, 1912
Mly113I GGCGCC 1 cut(s) 252
Mph1103I ATGCAT 1 cut(s) 703
MroXI GAANNNNTTC 1 cut(s) 2310
MseI TTAA 9 cut(s) 321, 651, 677, 722, 801, 1914, 2112, 2244, 2424
MslI CAYNNNNRTG 2 cut(s) 392, 611
MspA1I CMGCKG 3 cut(s) 21, 93, 2348
MspCI CTTAAG 1 cut(s) 676
MspI CCGG 5 cut(s) 156, 225, 878, 2162, 2891
MspR9I CCNGG 4 cut(s) 156, 977, 1245, 2225
MunI CAATTG 2 cut(s) 905, 1706
Mva1269I GAATGC 1 cut(s) 1530
MvaI CCWGG 3 cut(s) 977, 1245, 2225
MvnI CGCG 2 cut(s) 21, 205
NarI GGCGCC 1 cut(s) 252
NciI CCSGG 1 cut(s) 156
NlaIV GGNNCC 6 cut(s) 160, 253, 604, 1242, 1278, 1279
NmuCI GTSAC 4 cut(s) 311, 867, 2151, 2166
NotI GCGGCCGC 1 cut(s) 94
NsbI TGCGCA 2 cut(s) 990, 1541
NsiI ATGCAT 1 cut(s) 703
NspI RCATGY 2 cut(s) 705, 1550
PagI TCATGA 1 cut(s) 393
PceI AGGCCT 1 cut(s) 2130
PciI ACATGT 1 cut(s) 1546
PcsI WCGNNNNNNNCGW 3 cut(s) 255, 457, 1774
PctI GAATGC 1 cut(s) 1530
PdmI GAANNNNTTC 1 cut(s) 2310
PfeI GAWTC 7 cut(s) 616, 1157, 1265, 1692, 1987, 2306, 2887
PinAI ACCGGT 1 cut(s) 2161
PluTI GGCGCC 1 cut(s) 255
Ppu21I YACGTR 1 cut(s) 1769
PscI ACATGT 1 cut(s) 1546
Psp124BI GAGCTC 1 cut(s) 429
Psp1406I AACGTT 1 cut(s) 719
Psp6I CCWGG 3 cut(s) 975, 1243, 2223
PspFI CCCAGC 1 cut(s) 770
PspGI CCWGG 3 cut(s) 975, 1243, 2223
PspN4I GGNNCC 6 cut(s) 160, 253, 604, 1242, 1278, 1279
PspOMI GGGCCC 1 cut(s) 1276
PspPI GGNCC 6 cut(s) 13, 602, 1143, 1276, 1277, 1898
PsrI GAACNNNNNNTAC 2 cut(s) 2716, 2748
PstI CTGCAG 2 cut(s) 92, 478
PstNI CAGNNNCTG 1 cut(s) 2351
PsuI RGATCY 5 cut(s) 535, 760, 1947, 2501, 2751
PvuII CAGCTG 1 cut(s) 2348
RsaI GTAC 9 cut(s) 148, 706, 1151, 1218, 1225, 1868, 1942, 2010, 2031
RsaNI GTAC 9 cut(s) 147, 705, 1150, 1217, 1224, 1867, 1941, 2009, 2030
RseI CAYNNNNRTG 2 cut(s) 392, 611
SacI GAGCTC 1 cut(s) 429
SacII CCGCGG 1 cut(s) 22
SaqAI TTAA 9 cut(s) 321, 651, 677, 722, 801, 1914, 2112, 2244, 2424
Sau96I GGNCC 6 cut(s) 13, 602, 1143, 1276, 1277, 1898
SbfI CCTGCAGG 1 cut(s) 478
ScaI AGTACT 1 cut(s) 2010
ScrFI CCNGG 4 cut(s) 156, 977, 1245, 2225
SdaI CCTGCAGG 1 cut(s) 478
SduI GDGCHC 5 cut(s) 429, 1243, 1280, 1819, 1912
SfaNI GCATC 5 cut(s) 864, 1240, 1513, 1867, 2660
SfcI CTRYAG 5 cut(s) 88, 474, 1306, 2073, 2190
SfiI GGCCNNNNNGGCC 1 cut(s) 11
SfoI GGCGCC 1 cut(s) 253
Sfr303I CCGCGG 1 cut(s) 22
SgrBI CCGCGG 1 cut(s) 22
SmiMI CAYNNNNRTG 2 cut(s) 392, 611
SmlI CTYRAG 3 cut(s) 676, 1036, 1827
SmoI CTYRAG 3 cut(s) 676, 1036, 1827
Sse8387I CCTGCAGG 1 cut(s) 478
SseBI AGGCCT 1 cut(s) 2130
SspDI GGCGCC 1 cut(s) 251
SspI AATATT 2 cut(s) 985, 2647
SspMI CTAG 8 cut(s) 764, 2334, 2451, 2465, 2505, 2667, 2720, 2877
SstI GAGCTC 1 cut(s) 429
StuI AGGCCT 1 cut(s) 2130
StyD4I CCNGG 4 cut(s) 154, 975, 1243, 2223
StyI CCWWGG 1 cut(s) 306
TaiI ACGT 7 cut(s) 241, 566, 722, 967, 1771, 1862, 2041
TaqI TCGA 9 cut(s) 171, 192, 219, 633, 1849, 1888, 2013, 2272, 2794
TatI WGTACW 5 cut(s) 704, 1149, 1866, 2008, 2029
TauI GCSGC 3 cut(s) 24, 96, 99
TfiI GAWTC 7 cut(s) 616, 1157, 1265, 1692, 1987, 2306, 2887
Tru1I TTAA 9 cut(s) 321, 651, 677, 722, 801, 1914, 2112, 2244, 2424
Tru9I TTAA 9 cut(s) 321, 651, 677, 722, 801, 1914, 2112, 2244, 2424
TscAI CASTG 7 cut(s) 876, 943, 1071, 1444, 1542, 1819, 2412
TseFI GTSAC 4 cut(s) 311, 867, 2151, 2166
TseI GCWGC 8 cut(s) 90, 551, 569, 830, 1763, 2200, 2345, 2348
Tsp45I GTSAC 4 cut(s) 311, 867, 2151, 2166
TspDTI ATGAA 7 cut(s) 382, 1041, 1149, 1285, 2172, 2336, 2433
TspGWI ACGGA 4 cut(s) 582, 1552, 1960, 2185
TspRI CASTG 7 cut(s) 876, 943, 1071, 1444, 1542, 1819, 2412
Vha464I CTTAAG 1 cut(s) 676
VneI GTGCAC 1 cut(s) 1815
XapI RAATTY 4 cut(s) 1728, 1785, 1840, 2521
XbaI TCTAGA 1 cut(s) 763
XceI RCATGY 2 cut(s) 705, 1550
XcmI CCANNNNNNNNNTGG 1 cut(s) 2231
XmiI GTMKAC 1 cut(s) 1059
XmnI GAANNNNTTC 1 cut(s) 2310
XspI CTAG 8 cut(s) 764, 2334, 2451, 2465, 2505, 2667, 2720, 2877
ZrmI AGTACT 1 cut(s) 2010
Zsp2I ATGCAT 1 cut(s) 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.