Rw2G003770

Polyribonucleotide nucleotidyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004785
Physical Location & Seq
Forward (+)
3492285 .. 3494297
2013 bp
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UTR
Exon/CDS
Intron
Rw2G003770.1

Sequence Viewer

Length: 471 bp
ATGGAGAGAGAGATCAGTGCACCACGCACTCAAGATGATAAAAATTCTCCTCGATTAGCTATCTTGAAGTACAGCAATGATGCACTTCGTCGCTTGCTTGGGCCTCTGGGTGCTCTTAAAAGAAAAATTGAAGACAAAACAGGTACATGGATCTCTGTAAGTGATGGAACACTCACCATAGTTGCTAAGAATCAATCTCCAATGGACAAAGTACACGAAAAGGTTGATTTTGTACTTGGGCATGACATTGAAATTGGCGGGATCTATAAAGGGACTGTAGCTTCAATCAAAAAGTATGGTGCATTCGTGGAGTTTAATGGTGGGCAACAAGGCCTTCTACACATTTCTGAGTTGTCACATGAACCTGTGTTACGGATTTCAGATGCAGTATCTGTTGGCCAGCAGCTCTCTTTGATGTGCATAGGCCAGGATGTTTGTGGTAACATTAAGTTGTCCCTGAAAGCCACATAA

Protein Analysis

156

Amino Acids

17.0

Weight (kDa)

8.53

Isoelectric Point (pI)

35.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 84 - 154 5.8e-11 S1 RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 258
AclWI GGATC 2 cut(s) 158, 269
AcoI YGGCCR 1 cut(s) 397
AcsI RAATTY 1 cut(s) 43
AfaI GTAC 4 cut(s) 71, 145, 213, 234
AgsI TTSAA 4 cut(s) 67, 131, 251, 285
AjnI CCWGG 1 cut(s) 426
AluBI AGCT 3 cut(s) 59, 281, 406
AluI AGCT 3 cut(s) 59, 281, 406
Alw21I GWGCWC 2 cut(s) 22, 115
Alw44I GTGCAC 1 cut(s) 18
AlwI GGATC 2 cut(s) 158, 269
AlwNI CAGNNNCTG 1 cut(s) 392
AoxI GGCC 4 cut(s) 101, 331, 397, 424
ApaLI GTGCAC 1 cut(s) 18
ApeKI GCWGC 1 cut(s) 403
ApoI RAATTY 1 cut(s) 43
AspS9I GGNCC 1 cut(s) 101
AsuHPI GGTGA 1 cut(s) 166
BaeGI GKGCMC 1 cut(s) 22
BalI TGGCCA 1 cut(s) 399
BbsI GAAGAC 1 cut(s) 138
Bbv12I GWGCWC 2 cut(s) 22, 115
BbvI GCAGC 1 cut(s) 415
BccI CCATC 1 cut(s) 158
BciT130I CCWGG 1 cut(s) 428
BfmI CTRYAG 1 cut(s) 276
BisI GCNGC 1 cut(s) 404
BlsI GCNGC 1 cut(s) 405
Bme1390I CCNGG 1 cut(s) 428
BmgT120I GGNCC 1 cut(s) 101
BmrFI CCNGG 1 cut(s) 428
BmsI GCATC 2 cut(s) 70, 373
BpiI GAAGAC 1 cut(s) 138
BpuEI CTTGAG 1 cut(s) 15
Bse3DI GCAATG 1 cut(s) 82
BseBI CCWGG 1 cut(s) 428
BseGI GGATG 1 cut(s) 436
BseMI GCAATG 1 cut(s) 82
BseMII CTCAG 1 cut(s) 339
BseRI GAGGAG 1 cut(s) 39
BseSI GKGCMC 1 cut(s) 22
BseXI GCAGC 1 cut(s) 415
BshFI GGCC 4 cut(s) 103, 333, 399, 426
BsiHKAI GWGCWC 2 cut(s) 22, 115
BslFI GGGAC 2 cut(s) 286, 439
BsmFI GGGAC 2 cut(s) 286, 439
BsmI GAATGC 1 cut(s) 302
BsnI GGCC 4 cut(s) 103, 333, 399, 426
Bsp1286I GDGCHC 2 cut(s) 22, 115
Bsp143I GATC 3 cut(s) 12, 150, 261
BspACI CCGC 1 cut(s) 258
BspANI GGCC 4 cut(s) 103, 333, 399, 426
BspCNI CTCAG 1 cut(s) 340
BspPI GGATC 2 cut(s) 158, 269
BsrDI GCAATG 1 cut(s) 82
BssMI GATC 3 cut(s) 12, 150, 261
Bst2UI CCWGG 1 cut(s) 428
Bst4CI ACNGT 1 cut(s) 277
BstC8I GCNNGC 2 cut(s) 95, 401
BstDEI CTNAG 2 cut(s) 186, 348
BstF5I GGATG 1 cut(s) 436
BstKTI GATC 3 cut(s) 15, 153, 264
BstMBI GATC 3 cut(s) 12, 150, 261
BstNI CCWGG 1 cut(s) 428
BstSCI CCNGG 1 cut(s) 426
BstSFI CTRYAG 1 cut(s) 276
BstSLI GKGCMC 1 cut(s) 22
BstV1I GCAGC 1 cut(s) 415
BstV2I GAAGAC 1 cut(s) 138
BstX2I RGATCY 2 cut(s) 150, 261
BstYI RGATCY 2 cut(s) 150, 261
BsuRI GGCC 4 cut(s) 103, 333, 399, 426
BtsCI GGATG 1 cut(s) 436
BtsIMutI CAGTG 1 cut(s) 22
Cac8I GCNNGC 2 cut(s) 95, 401
CaiI CAGNNNCTG 1 cut(s) 392
Cfr13I GGNCC 1 cut(s) 101
Csp6I GTAC 4 cut(s) 70, 144, 212, 233
CviAII CATG 3 cut(s) 147, 242, 359
CviJI RGCY 8 cut(s) 59, 103, 281, 333, 399, 406, 426, 464
CviKI_1 RGCY 8 cut(s) 59, 103, 281, 333, 399, 406, 426, 464
CviQI GTAC 4 cut(s) 70, 144, 212, 233
DdeI CTNAG 2 cut(s) 186, 348
DpnI GATC 3 cut(s) 14, 152, 263
DpnII GATC 3 cut(s) 12, 150, 261
EaeI YGGCCR 1 cut(s) 397
Eco147I AGGCCT 1 cut(s) 333
EcoRII CCWGG 1 cut(s) 426
FaeI CATG 3 cut(s) 150, 245, 362
FaiI YATR 8 cut(s) 148, 179, 243, 267, 297, 360, 422, 469
FaqI GGGAC 2 cut(s) 286, 439
FatI CATG 3 cut(s) 146, 241, 358
FauI CCCGC 1 cut(s) 251
Fnu4HI GCNGC 1 cut(s) 404
FokI GGATG 1 cut(s) 443
Fsp4HI GCNGC 1 cut(s) 404
GluI GCNGC 1 cut(s) 404
HaeIII GGCC 4 cut(s) 103, 333, 399, 426
Hin1II CATG 3 cut(s) 150, 245, 362
HinfI GANTC 1 cut(s) 190
HphI GGTGA 1 cut(s) 166
Hpy166II GTNNAC 2 cut(s) 20, 214
Hpy188I TCNGA 2 cut(s) 349, 382
Hpy188III TCNNGA 2 cut(s) 32, 64
Hpy8I GTNNAC 2 cut(s) 20, 214
Hpy99I CGWCG 1 cut(s) 93
HpyAV CCTTC 1 cut(s) 344
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4V TGCA 5 cut(s) 20, 83, 302, 386, 420
HpyF3I CTNAG 2 cut(s) 186, 348
Hsp92II CATG 3 cut(s) 150, 245, 362
Kzo9I GATC 3 cut(s) 12, 150, 261
LpnPI CCDG 6 cut(s) 92, 126, 378, 413, 413, 440
Lsp1109I GCAGC 1 cut(s) 415
LweI GCATC 2 cut(s) 70, 373
MaeIII GTNAC 3 cut(s) 354, 369, 440
MalI GATC 3 cut(s) 14, 152, 263
MboI GATC 3 cut(s) 12, 150, 261
MboII GAAGA 1 cut(s) 143
MflI RGATCY 2 cut(s) 150, 261
MhlI GDGCHC 2 cut(s) 22, 115
MlsI TGGCCA 1 cut(s) 399
MluCI AATT 3 cut(s) 43, 126, 252
MluNI TGGCCA 1 cut(s) 399
MnlI CCTC 2 cut(s) 60, 114
Mox20I TGGCCA 1 cut(s) 399
MscI TGGCCA 1 cut(s) 399
MseI TTAA 3 cut(s) 117, 315, 447
Msp20I TGGCCA 1 cut(s) 399
MspR9I CCNGG 1 cut(s) 428
Mva1269I GAATGC 1 cut(s) 302
MvaI CCWGG 1 cut(s) 428
NdeII GATC 3 cut(s) 12, 150, 261
NlaIII CATG 3 cut(s) 150, 245, 362
NmuCI GTSAC 1 cut(s) 354
PceI AGGCCT 1 cut(s) 333
PctI GAATGC 1 cut(s) 302
PfeI GAWTC 1 cut(s) 190
PkrI GCNGC 1 cut(s) 405
Psp6I CCWGG 1 cut(s) 426
PspGI CCWGG 1 cut(s) 426
PspPI GGNCC 1 cut(s) 101
PsrI GAACNNNNNNTAC 2 cut(s) 354, 386
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 2 cut(s) 150, 261
RsaI GTAC 4 cut(s) 71, 145, 213, 234
RsaNI GTAC 4 cut(s) 70, 144, 212, 233
SaqAI TTAA 3 cut(s) 117, 315, 447
SatI GCNGC 1 cut(s) 404
Sau3AI GATC 3 cut(s) 12, 150, 261
Sau96I GGNCC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 428
SduI GDGCHC 2 cut(s) 22, 115
SetI ASST 6 cut(s) 61, 145, 225, 283, 367, 408
SfaNI GCATC 2 cut(s) 70, 373
SfcI CTRYAG 1 cut(s) 276
SmlI CTYRAG 1 cut(s) 30
SmoI CTYRAG 1 cut(s) 30
Sse9I AATT 3 cut(s) 43, 126, 252
SseBI AGGCCT 1 cut(s) 333
SsiI CCGC 1 cut(s) 258
StuI AGGCCT 1 cut(s) 333
StyD4I CCNGG 1 cut(s) 426
TaaI ACNGT 1 cut(s) 277
TaqI TCGA 1 cut(s) 52
TasI AATT 3 cut(s) 43, 126, 252
TatI WGTACW 3 cut(s) 69, 211, 232
TfiI GAWTC 1 cut(s) 190
Tru1I TTAA 3 cut(s) 117, 315, 447
Tru9I TTAA 3 cut(s) 117, 315, 447
TscAI CASTG 1 cut(s) 22
TseFI GTSAC 1 cut(s) 354
TseI GCWGC 1 cut(s) 403
Tsp45I GTSAC 1 cut(s) 354
TspDTI ATGAA 1 cut(s) 375
TspGWI ACGGA 1 cut(s) 388
TspRI CASTG 1 cut(s) 22
VneI GTGCAC 1 cut(s) 18
XapI RAATTY 1 cut(s) 43
XcmI CCANNNNNNNNNTGG 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.