FvH4_7g01760

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007095
Physical Location & Seq
Forward (+)
2152268 .. 2156350
4083 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01760.t2

Sequence Viewer

Length: 1518 bp
ATGAATATTCCTAGGCCATGTTTGGTTCTTCTCTCTTCTTTTCTCCTTCTTTCTTGCTTCTCCAATATCCCTCAAATCCATGGGAGCAAGCATTCACAAGCGTTTTCTCAGCTTTACAAGTCCAAGCTGAATGCAGAAAGCGCAGGCGTCGACACGAAGCCATTCGAGGTGATTCTTCATGCGAATGCGAGCAGCATTCATCATCCCCAGGAAGGGTTGAAGGAGAAAGATAGGATCACCAGCTTGCCTGGACAACCAAAGGTGAACTTCAATCAGTATGGTGGGTATGTTACTGTCGATAAAAAAGCCGGTCGTGCCTTTTTTTACTACTTTGCTGAAGCTGAGGATCAGCAGGCTAAGGATTCTTTGCCTCTTCTTCTATGGCTCAATGGAGGACCTGGATGTTCCTCTCTTGCCTATGGAGCAATGCTTGAGCTTGGACCGTTCCGTATCCACAGCGATGGCAAGACCCTTTACAGAAACAGATTTTCATGGAACTATGCTGCAAATGTTTTGTTCCTTGAGTCTCCTGCTGGTGTGGGGTTTTCATACTCTAACACAACATCAGACTATGATAAAAGTGGGGACAGAAGAACAGCAGCAGATAATTATGTGTTCTTGGTGAATTGGTTGGAGAGGTTTCCTGAATATAAGGGGAGGGAGTTCTATATTTCTGGTGAAAGCTATGCAGGGCACTATGTGCCTCAACTTGCACAGACTATCCTCCACCACAACAAGAAGGCTAACAAAACCATCATCAACCTCAAGGGCATAATTATTGGGAATGCAGTGATTAACGATGAAACTGATACAAAAGGAATGTATGACTACCTTGCTACTCACGCTCTCATTTCCGATGAAATTGCAAATCAAGTCCACAAATACTGTGATTTTTCACCCAATTCCACTACTGATGAAACCAAGGAGTGCGGTGATGCTATGGATGCTATTGACAGGGCTACCTATTACATTGATATCTATAACATCTACGCCCCCTTATGCACCTCTTCCAATCTCACTGTCAAACCCAAAAAGGCTTCAATACTGAAATTTGATCCGTGTAGCGAGAACTATGCGTATGCGTATTTGAATCGGCCTGATGTTCAAGAAGCTCTCCATGCTAATGTCACTAAACTCTCACATGACTGGGAACCATGCAGTGATGTAATTACACATTGGGGAGATAGCTCAACAACTATACTACCCCTTTTACATGAGTTTATGGACAATGGACTTCGAGTCTGGATTTTTAGTGGCGATACCGATGGAAGGGTTCCAGTCACTTCAACAAAGTATTCTCTTGAGAAGATGAATTTACCAATCAAGACTGCATGGCACGCTTGGTTCCTTAGCGGACAGGTTGGTGGTTACACACAAGTGTATGAAGGAGACATAACATTTGCCACAGTGAGGGAGGCAGGGCATCAAGTCCCAAGCTACCAGCCTGCTAGGGCACTTTCACTCATCAAGCACTTCCTTGATGGCACACCTCTTCCTGATACTACAAGATATACTTGA

Protein Analysis

506

Amino Acids

56.6

Weight (kDa)

6.13

Isoelectric Point (pI)

36.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 83 - 261 5.3e-80 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 150
AciI CCGC 2 cut(s) 930, 1353
AclWI GGATC 3 cut(s) 242, 354, 1049
AcsI RAATTY 2 cut(s) 1049, 1312
AcuI CTGAAG 1 cut(s) 357
AcyI GRCGYC 1 cut(s) 147
AdeI CACNNNGTG 1 cut(s) 700
AfiI CCNNNNNNNGG 4 cut(s) 212, 213, 1269, 1410
AgsI TTSAA 6 cut(s) 220, 271, 1041, 1090, 1106, 1287
AhdI GACNNNNNGTC 1 cut(s) 1238
AjnI CCWGG 3 cut(s) 207, 247, 397
AleI CACNNNNGTG 1 cut(s) 1376
AluBI AGCT 9 cut(s) 112, 127, 243, 341, 436, 684, 1112, 1188, 1437
AluI AGCT 9 cut(s) 112, 127, 243, 341, 436, 684, 1112, 1188, 1437
Alw26I GTCTC 2 cut(s) 531, 1383
AlwI GGATC 3 cut(s) 242, 354, 1049
AoxI GGCC 2 cut(s) 14, 1094
ApeKI GCWGC 3 cut(s) 192, 503, 599
ApoI RAATTY 2 cut(s) 1049, 1312
ArsI GACNNNNNNTTYG 2 cut(s) 1382, 1414
Asp700I GAANNNNTTC 1 cut(s) 161
AspA2I CCTAGG 1 cut(s) 11
AspLEI GCGC 1 cut(s) 143
AspS9I GGNCC 2 cut(s) 395, 440
AsuHPI GGTGA 7 cut(s) 181, 229, 274, 634, 689, 888, 944
AvaII GGWCC 2 cut(s) 395, 440
AvrII CCTAGG 1 cut(s) 11
BaeGI GKGCMC 2 cut(s) 696, 1456
BbvCI CCTCAGC 1 cut(s) 342
BbvI GCAGC 3 cut(s) 204, 490, 611
BccI CCATC 4 cut(s) 455, 761, 1259, 1475
BcgI CGANNNNNNTGC 4 cut(s) 845, 879, 1055, 1089
BciT130I CCWGG 3 cut(s) 209, 249, 399
BciVI GTATCC 1 cut(s) 461
BcoDI GTCTC 2 cut(s) 531, 1383
BfaI CTAG 2 cut(s) 12, 1449
BfuI GTATCC 1 cut(s) 461
BisI GCNGC 3 cut(s) 193, 504, 600
BlnI CCTAGG 1 cut(s) 11
BlsI GCNGC 3 cut(s) 194, 505, 601
Bme1390I CCNGG 3 cut(s) 209, 249, 399
Bme18I GGWCC 2 cut(s) 395, 440
BmeRI GACNNNNNGTC 1 cut(s) 1238
BmgT120I GGNCC 2 cut(s) 395, 440
BmiI GGNNCC 3 cut(s) 1152, 1275, 1346
BmrFI CCNGG 3 cut(s) 209, 249, 399
BmrI ACTGGG 1 cut(s) 1156
BmsI GCATC 3 cut(s) 925, 934, 1432
BmuI ACTGGG 1 cut(s) 1156
Bpu10I CCTNAGC 3 cut(s) 342, 357, 1349
BpuEI CTTGAG 4 cut(s) 452, 542, 749, 1322
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 4 cut(s) 11, 79, 207, 921
Bsc4I CCNNNNNNNGG 4 cut(s) 212, 213, 1269, 1410
Bse118I RCCGGY 1 cut(s) 308
Bse1I ACTGG 2 cut(s) 1151, 1277
Bse3DI GCAATG 1 cut(s) 432
BseBI CCWGG 3 cut(s) 209, 249, 399
BseDI CCNNGG 4 cut(s) 11, 79, 207, 921
BseGI GGATG 3 cut(s) 202, 407, 949
BseLI CCNNNNNNNGG 4 cut(s) 212, 213, 1269, 1410
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 2 cut(s) 122, 333
BseNI ACTGG 2 cut(s) 1151, 1277
BseSI GKGCMC 2 cut(s) 696, 1456
BseXI GCAGC 3 cut(s) 204, 490, 611
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 2 cut(s) 16, 1096
BsiEI CGRYCG 1 cut(s) 313
BsiSI CCGG 1 cut(s) 309
BslFI GGGAC 2 cut(s) 599, 1415
BslI CCNNNNNNNGG 4 cut(s) 212, 213, 1269, 1410
BsmAI GTCTC 2 cut(s) 531, 1383
BsmFI GGGAC 2 cut(s) 599, 1415
BsmI GAATGC 5 cut(s) 91, 136, 190, 195, 790
BsnI GGCC 2 cut(s) 16, 1096
Bsp1286I GDGCHC 2 cut(s) 696, 1456
Bsp143I GATC 3 cut(s) 234, 346, 1054
Bsp19I CCATGG 1 cut(s) 79
BspACI CCGC 2 cut(s) 930, 1353
BspANI GGCC 2 cut(s) 16, 1096
BspCNI CTCAG 2 cut(s) 121, 334
BspLI GGNNCC 3 cut(s) 1152, 1275, 1346
BspPI GGATC 3 cut(s) 242, 354, 1049
BsrDI GCAATG 1 cut(s) 432
BsrFI RCCGGY 1 cut(s) 308
BsrI ACTGG 2 cut(s) 1151, 1277
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 4 cut(s) 11, 79, 207, 921
BssMI GATC 3 cut(s) 234, 346, 1054
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 3 cut(s) 11, 79, 921
Bst2UI CCWGG 3 cut(s) 209, 249, 399
Bst4CI ACNGT 5 cut(s) 295, 444, 887, 1021, 1408
Bst6I CTCTTC 4 cut(s) 40, 378, 1012, 1497
BstACI GRCGYC 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 700
BstC8I GCNNGC 7 cut(s) 89, 145, 190, 245, 354, 1338, 1446
BstDEI CTNAG 4 cut(s) 108, 342, 357, 1349
BstDSI CCRYGG 1 cut(s) 79
BstF5I GGATG 3 cut(s) 202, 407, 949
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 3 cut(s) 237, 349, 1057
BstMAI GTCTC 2 cut(s) 531, 1383
BstMBI GATC 3 cut(s) 234, 346, 1054
BstMCI CGRYCG 1 cut(s) 313
BstMWI GCNNNNNNNGC 8 cut(s) 140, 314, 422, 700, 842, 944, 1118, 1337
BstNI CCWGG 3 cut(s) 209, 249, 399
BstSCI CCNGG 3 cut(s) 207, 247, 397
BstSLI GKGCMC 2 cut(s) 696, 1456
BstV1I GCAGC 3 cut(s) 204, 490, 611
BstXI CCANNNNNNTGG 1 cut(s) 461
BsuI GTATCC 1 cut(s) 461
BsuRI GGCC 2 cut(s) 16, 1096
BtgI CCRYGG 1 cut(s) 79
BtgZI GCGATG 1 cut(s) 474
BtsCI GGATG 3 cut(s) 202, 407, 949
BtsI GCAGTG 2 cut(s) 795, 1165
BtsIMutI CAGTG 4 cut(s) 795, 1017, 1165, 1413
Cac8I GCNNGC 7 cut(s) 89, 145, 190, 245, 354, 1338, 1446
CfoI GCGC 1 cut(s) 143
Cfr10I RCCGGY 1 cut(s) 308
Cfr13I GGNCC 2 cut(s) 395, 440
CseI GACGC 1 cut(s) 136
CviAII CATG 9 cut(s) 18, 80, 179, 492, 1118, 1142, 1155, 1214, 1332
DdeI CTNAG 4 cut(s) 108, 342, 357, 1349
DpnI GATC 3 cut(s) 236, 348, 1056
DpnII GATC 3 cut(s) 234, 346, 1054
DraIII CACNNNGTG 1 cut(s) 700
DriI GACNNNNNGTC 1 cut(s) 1238
Eam1104I CTCTTC 4 cut(s) 40, 378, 1012, 1497
Eam1105I GACNNNNNGTC 1 cut(s) 1238
EarI CTCTTC 4 cut(s) 40, 378, 1012, 1497
Eco130I CCWWGG 3 cut(s) 11, 79, 921
Eco32I GATATC 1 cut(s) 976
Eco47I GGWCC 2 cut(s) 395, 440
Eco57I CTGAAG 1 cut(s) 357
EcoO109I RGGNCCY 1 cut(s) 395
EcoRII CCWGG 3 cut(s) 207, 247, 397
EcoRV GATATC 1 cut(s) 976
EcoT14I CCWWGG 3 cut(s) 11, 79, 921
ErhI CCWWGG 3 cut(s) 11, 79, 921
FaeI CATG 9 cut(s) 21, 83, 182, 495, 1121, 1145, 1158, 1217, 1335
FalI AAGNNNNNCTT 3 cut(s) 251, 283, 1498
FaqI GGGAC 2 cut(s) 599, 1415
FatI CATG 9 cut(s) 17, 79, 178, 491, 1117, 1141, 1154, 1213, 1331
FblI GTMKAC 1 cut(s) 150
Fnu4HI GCNGC 3 cut(s) 193, 504, 600
FokI GGATG 3 cut(s) 189, 414, 956
Fsp4HI GCNGC 3 cut(s) 193, 504, 600
FspBI CTAG 2 cut(s) 12, 1449
GlaI GCGC 1 cut(s) 142
GluI GCNGC 3 cut(s) 193, 504, 600
HaeIII GGCC 2 cut(s) 16, 1096
HapII CCGG 1 cut(s) 309
HgaI GACGC 1 cut(s) 136
HhaI GCGC 1 cut(s) 143
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 9 cut(s) 21, 83, 182, 495, 1121, 1145, 1158, 1217, 1335
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HinfI GANTC 5 cut(s) 172, 362, 524, 1090, 1239
HpaII CCGG 1 cut(s) 309
HphI GGTGA 7 cut(s) 181, 229, 274, 634, 689, 888, 944
Hpy166II GTNNAC 3 cut(s) 151, 265, 877
Hpy188I TCNGA 2 cut(s) 568, 856
Hpy188III TCNNGA 6 cut(s) 644, 1106, 1243, 1301, 1324, 1496
Hpy8I GTNNAC 3 cut(s) 151, 265, 877
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 6 cut(s) 56, 206, 214, 733, 1263, 1379
HpyCH4III ACNGT 5 cut(s) 295, 444, 887, 1021, 1408
HpyCH4V TGCA 9 cut(s) 134, 506, 689, 713, 788, 866, 1002, 1158, 1331
HpyF10VI GCNNNNNNNGC 8 cut(s) 140, 314, 422, 700, 842, 944, 1118, 1337
HpyF3I CTNAG 4 cut(s) 108, 342, 357, 1349
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 9 cut(s) 21, 83, 182, 495, 1121, 1145, 1158, 1217, 1335
HspAI GCGC 1 cut(s) 141
Kzo9I GATC 3 cut(s) 234, 346, 1054
LmnI GCTCC 2 cut(s) 84, 422
Lsp1109I GCAGC 3 cut(s) 204, 490, 611
LweI GCATC 3 cut(s) 925, 934, 1432
MaeI CTAG 2 cut(s) 12, 1449
MaeIII GTNAC 4 cut(s) 289, 1126, 1279, 1367
MalI GATC 3 cut(s) 236, 348, 1056
MboI GATC 3 cut(s) 234, 346, 1054
MboII GAAGA 9 cut(s) 20, 27, 167, 365, 368, 603, 999, 1318, 1484
MhlI GDGCHC 2 cut(s) 696, 1456
MluCI AATT 8 cut(s) 607, 625, 774, 861, 901, 1049, 1167, 1312
MlyI GAGTC 2 cut(s) 533, 1248
MmeI TCCRAC 1 cut(s) 612
MroXI GAANNNNTTC 1 cut(s) 161
MseI TTAA 1 cut(s) 795
MslI CAYNNNNRTG 4 cut(s) 183, 459, 1122, 1376
MspI CCGG 1 cut(s) 309
MspR9I CCNGG 3 cut(s) 209, 249, 399
Mva1269I GAATGC 5 cut(s) 91, 136, 190, 195, 790
MvaI CCWGG 3 cut(s) 209, 249, 399
MwoI GCNNNNNNNGC 8 cut(s) 140, 314, 422, 700, 842, 944, 1118, 1337
NcoI CCATGG 1 cut(s) 79
NdeII GATC 3 cut(s) 234, 346, 1054
NlaIII CATG 9 cut(s) 21, 83, 182, 495, 1121, 1145, 1158, 1217, 1335
NlaIV GGNNCC 3 cut(s) 1152, 1275, 1346
NmuCI GTSAC 2 cut(s) 1126, 1279
OliI CACNNNNGTG 1 cut(s) 1376
PctI GAATGC 5 cut(s) 91, 136, 190, 195, 790
PdmI GAANNNNTTC 1 cut(s) 161
PfeI GAWTC 3 cut(s) 172, 362, 1090
PkrI GCNGC 3 cut(s) 194, 505, 601
PleI GAGTC 2 cut(s) 532, 1247
PpsI GAGTC 2 cut(s) 532, 1247
PpuMI RGGWCCY 1 cut(s) 395
Psp5II RGGWCCY 1 cut(s) 395
Psp6I CCWGG 3 cut(s) 207, 247, 397
PspGI CCWGG 3 cut(s) 207, 247, 397
PspN4I GGNNCC 3 cut(s) 1152, 1275, 1346
PspPI GGNCC 2 cut(s) 395, 440
PspPPI RGGWCCY 1 cut(s) 395
RseI CAYNNNNRTG 4 cut(s) 183, 459, 1122, 1376
SalI GTCGAC 1 cut(s) 149
SaqAI TTAA 1 cut(s) 795
SatI GCNGC 3 cut(s) 193, 504, 600
Sau3AI GATC 3 cut(s) 234, 346, 1054
Sau96I GGNCC 2 cut(s) 395, 440
SchI GAGTC 2 cut(s) 533, 1248
ScrFI CCNGG 3 cut(s) 209, 249, 399
SduI GDGCHC 2 cut(s) 696, 1456
SfaNI GCATC 3 cut(s) 925, 934, 1432
SinI GGWCC 2 cut(s) 395, 440
SmiMI CAYNNNNRTG 4 cut(s) 183, 459, 1122, 1376
SmlI CTYRAG 4 cut(s) 431, 521, 764, 1301
SmoI CTYRAG 4 cut(s) 431, 521, 764, 1301
Sse9I AATT 8 cut(s) 607, 625, 774, 861, 901, 1049, 1167, 1312
SsiI CCGC 2 cut(s) 930, 1353
SspI AATATT 1 cut(s) 7
SspMI CTAG 2 cut(s) 12, 1449
StyD4I CCNGG 3 cut(s) 207, 247, 397
StyI CCWWGG 3 cut(s) 11, 79, 921
TaaI ACNGT 5 cut(s) 295, 444, 887, 1021, 1408
TaqI TCGA 4 cut(s) 150, 165, 297, 1237
TasI AATT 8 cut(s) 607, 625, 774, 861, 901, 1049, 1167, 1312
TfiI GAWTC 3 cut(s) 172, 362, 1090
Tru1I TTAA 1 cut(s) 795
Tru9I TTAA 1 cut(s) 795
TscAI CASTG 4 cut(s) 795, 1024, 1165, 1413
TseFI GTSAC 2 cut(s) 1126, 1279
TseI GCWGC 3 cut(s) 192, 503, 599
Tsp45I GTSAC 2 cut(s) 1126, 1279
TspGWI ACGGA 2 cut(s) 437, 1047
TspRI CASTG 4 cut(s) 795, 1024, 1165, 1413
VpaK11BI GGWCC 2 cut(s) 395, 440
XapI RAATTY 2 cut(s) 1049, 1312
XmaJI CCTAGG 1 cut(s) 11
XmiI GTMKAC 1 cut(s) 150
XmnI GAANNNNTTC 1 cut(s) 161
XspI CTAG 2 cut(s) 12, 1449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.