MD07G1023100.v1.1

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007095
Physical Location & Seq
Forward (+)
1930135 .. 1935262
5128 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1023100.v1.1.491

Sequence Viewer

Length: 1509 bp
ATGAATACAATCAAAATGCAAATAATTAGGCCTTGTTTGGCAATTTTCTCTCTTCTCATTCTTTCTGGCTTCGCAACTCAAATCCATGGAAGCAAGCAATCGCAAGCACTTTCACAGCTCCACAGATCCAAATTGAAGAACGTCGCCCACATCGACAAGAGCCCCTTCGTGGCAATTCTTCACGCAAACGCAACCAAAACTCATCCTCAGGAAGGGTTGAAGGTGAAGGATAGGATCGTTAAATTGCCTGGACAACCCAACGTGAAGTTCTCTCAGTATGGTGGGTACGTTACGGTGGATAAAAAAGCCGGCCGTGCAATGTTTTACTATTTAGTTGAAGCTGAGCAAGCTAAGGATTCTTCACCTCTTCTTCTGTGGCTCAATGGAGGACCTGGTTGTTCTTCTCTTGCCTATGGAGCAATGCTCGAGCTTGGACCATTCCGAATCCACAGCGATGGAAAAACACTTTACAGAAACAGATTTTCATGGAATCATGCTGCTAATGTGTTGTTCCTTGAGTCTCCTGCTGGGGTGGGATTTTCATACTCAAATAGAACATCAGATTACGATGCAAGCGGGGATAGAAACACAGCTGCAGATAATTATGTATTTTTGGTGAATTGGTTGGAGAGGTTTCCTGAATACAAGGGCAGGGAGTTTTATATCTCTGGGGAAAGTTATGCAGGGCATTATGTACCTCAACTTGCACATACCATTCTCTACCATAATAAAAGGGCTAACAAGACCATTATCAACCTCAAGGGCATAATAATTGGGAATGCAGTGATCAATGATGAAACTGATACAAAAGGAATGTACGACTACCTTGCGAGCCATGCTATCATTTCAGATAAAACTGCAAATGATATTAATAAGTACTGTAATTTTGCACCTACTGTCACTACCGATCAGCATAAGGAGTGCAGTGATGCCACTGATGCTGCTGCTAAAGATACCTATTACGTTGACATTTATAACATCTATGCCCCTTCATGTTTGTCTACCAACCTCACTGCAAAACCCAAAAAGACTTCAATATTCAAATTTGATCCGTGTAGTGAGGACTATGCGTATGCATATTTGAATCGGCCTGACGTTCAACAAGCTCTCCATGCCAATGTCACCAAACTCTCTCACGATTGGGAACCATGTAGTGATGTTATCACATGGGGCGATAGCGCATCAACCGTACTACCTCTTTTACATGAGTTCCTGGAGAATGGACTTCGAGTGTGGATTTTCAGTGGCGACATTGATGGGAGGGTACCAGTCACTTCAACAAAGTATTCTCTTGACAAGATGAATCTTCCAGTAAAGACTGAGTGGCACGCCTGGTTCCTTAGTGGAGAGGTTGCTGGGTACACACAAGTGTTCGAGGGAGGCCTAACATTTGCTACAGTGAGACACGCAGGGCATCAAGTTCCAAGCTATCAGCCTGCAAGAGGACTTTCACTCATCAAGCACTTCCTTGATGGCACACCTCTTCCCGATACTAAAAGATATAACTAG

Protein Analysis

503

Amino Acids

56.08

Weight (kDa)

8.26

Isoelectric Point (pI)

37.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 83 - 492 3.1e-144 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 975
Acc65I GGTACC 1 cut(s) 1264
AccB1I GGYRCC 1 cut(s) 1264
AccI GTMKAC 1 cut(s) 1001
AciI CCGC 1 cut(s) 576
AclWI GGATC 3 cut(s) 120, 242, 1043
AcoI YGGCCR 1 cut(s) 310
AcsI RAATTY 1 cut(s) 1043
AfaI GTAC 7 cut(s) 287, 696, 818, 878, 1191, 1266, 1361
AfiI CCNNNNNNNGG 3 cut(s) 169, 212, 1442
AgsI TTSAA 8 cut(s) 136, 220, 338, 1035, 1042, 1084, 1100, 1278
AjnI CCWGG 4 cut(s) 247, 391, 1212, 1331
AjuI GAANNNNNNNTTGG 2 cut(s) 251, 283
AleI CACNNNNGTG 1 cut(s) 1367
AluBI AGCT 7 cut(s) 118, 341, 350, 430, 593, 1106, 1428
AluI AGCT 7 cut(s) 118, 341, 350, 430, 593, 1106, 1428
Alw26I GTCTC 2 cut(s) 525, 1396
AlwI GGATC 3 cut(s) 120, 242, 1043
Ama87I CYCGRG 1 cut(s) 425
AoxI GGCC 4 cut(s) 29, 310, 1088, 1381
ApeKI GCWGC 4 cut(s) 497, 593, 941, 944
ApoI RAATTY 1 cut(s) 1043
AseI ATTAAT 1 cut(s) 870
Asp718I GGTACC 1 cut(s) 1264
AspLEI GCGC 1 cut(s) 1181
AspS9I GGNCC 2 cut(s) 389, 434
AsuHPI GGTGA 4 cut(s) 235, 354, 628, 1114
AvaI CYCGRG 1 cut(s) 425
AvaII GGWCC 2 cut(s) 389, 434
AxyI CCTNAGG 1 cut(s) 207
BanI GGYRCC 1 cut(s) 1264
BanII GRGCYC 1 cut(s) 164
BbvI GCAGC 4 cut(s) 484, 580, 928, 931
BccI CCATC 3 cut(s) 449, 1250, 1466
BceAI ACGGC 1 cut(s) 297
BcgI CGANNNNNNTGC 2 cut(s) 809, 843
BciT130I CCWGG 4 cut(s) 249, 393, 1214, 1333
BclI TGATCA 1 cut(s) 786
BcoDI GTCTC 2 cut(s) 525, 1396
BfaI CTAG 1 cut(s) 1507
BfmI CTRYAG 2 cut(s) 594, 1395
BisI GCNGC 4 cut(s) 498, 594, 942, 945
BlpI GCTNAGC 1 cut(s) 342
BlsI GCNGC 4 cut(s) 499, 595, 943, 946
BmcAI AGTACT 1 cut(s) 878
Bme1390I CCNGG 4 cut(s) 249, 393, 1214, 1333
Bme18I GGWCC 2 cut(s) 389, 434
BmeT110I CYCGRG 1 cut(s) 425
BmgT120I GGNCC 2 cut(s) 389, 434
BmiI GGNNCC 3 cut(s) 1146, 1266, 1337
BmrFI CCNGG 4 cut(s) 249, 393, 1214, 1333
BmsI GCATC 5 cut(s) 559, 919, 928, 1190, 1423
BplI GAGNNNNNCTC 2 cut(s) 408, 440
BpmI CTGGAG 1 cut(s) 1235
Bpu10I CCTNAGC 1 cut(s) 351
Bpu1102I GCTNAGC 1 cut(s) 342
BpuEI CTTGAG 2 cut(s) 536, 743
BsaJI CCNNGG 1 cut(s) 85
BsaXI ACNNNNNCTCC 2 cut(s) 1092, 1122
Bsc4I CCNNNNNNNGG 3 cut(s) 169, 212, 1442
Bse118I RCCGGY 1 cut(s) 308
Bse1I ACTGG 2 cut(s) 1268, 1310
Bse21I CCTNAGG 1 cut(s) 207
Bse3DI GCAATG 2 cut(s) 324, 426
BseBI CCWGG 4 cut(s) 249, 393, 1214, 1333
BseDI CCNNGG 1 cut(s) 85
BseGI GGATG 1 cut(s) 202
BseLI CCNNNNNNNGG 3 cut(s) 169, 212, 1442
BseMI GCAATG 2 cut(s) 324, 426
BseMII CTCAG 4 cut(s) 221, 287, 333, 1311
BseNI ACTGG 2 cut(s) 1268, 1310
BseX3I CGGCCG 1 cut(s) 310
BseXI GCAGC 4 cut(s) 484, 580, 928, 931
BseYI CCCAGC 2 cut(s) 527, 1355
BsgI GTGCAG 1 cut(s) 943
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 4 cut(s) 31, 312, 1090, 1383
BshNI GGYRCC 1 cut(s) 1264
BsiEI CGRYCG 1 cut(s) 313
BsiHKCI CYCGRG 1 cut(s) 425
BsiSI CCGG 1 cut(s) 309
BslI CCNNNNNNNGG 3 cut(s) 169, 212, 1442
BsmAI GTCTC 2 cut(s) 525, 1396
BsmI GAATGC 1 cut(s) 784
BsnI GGCC 4 cut(s) 31, 312, 1090, 1383
BsoBI CYCGRG 1 cut(s) 425
Bsp1286I GDGCHC 1 cut(s) 164
Bsp143I GATC 5 cut(s) 125, 234, 786, 907, 1048
Bsp1720I GCTNAGC 1 cut(s) 342
Bsp19I CCATGG 1 cut(s) 85
BspACI CCGC 1 cut(s) 576
BspANI GGCC 4 cut(s) 31, 312, 1090, 1383
BspCNI CTCAG 4 cut(s) 220, 286, 334, 1312
BspLI GGNNCC 3 cut(s) 1146, 1266, 1337
BspMAI CTGCAG 1 cut(s) 598
BspPI GGATC 3 cut(s) 120, 242, 1043
BspT107I GGYRCC 1 cut(s) 1264
BsrDI GCAATG 2 cut(s) 324, 426
BsrFI RCCGGY 1 cut(s) 308
BsrI ACTGG 2 cut(s) 1268, 1310
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 1 cut(s) 85
BssMI GATC 5 cut(s) 125, 234, 786, 907, 1048
BssT1I CCWWGG 1 cut(s) 85
Bst2UI CCWGG 4 cut(s) 249, 393, 1214, 1333
Bst4CI ACNGT 5 cut(s) 295, 881, 898, 1189, 1399
Bst6I CTCTTC 3 cut(s) 57, 372, 1488
BstC8I GCNNGC 8 cut(s) 95, 105, 310, 348, 574, 832, 1329, 1437
BstDEI CTNAG 6 cut(s) 207, 273, 342, 351, 1320, 1340
BstDSI CCRYGG 1 cut(s) 85
BstENI CCTNNNNNAGG 2 cut(s) 210, 1440
BstF5I GGATG 1 cut(s) 202
BstHHI GCGC 1 cut(s) 1181
BstKTI GATC 5 cut(s) 128, 237, 789, 910, 1051
BstMAI GTCTC 2 cut(s) 525, 1396
BstMBI GATC 5 cut(s) 125, 234, 786, 907, 1048
BstMCI CGRYCG 1 cut(s) 313
BstMWI GCNNNNNNNGC 6 cut(s) 314, 347, 416, 836, 938, 1112
BstNI CCWGG 4 cut(s) 249, 393, 1214, 1333
BstSCI CCNGG 4 cut(s) 247, 391, 1212, 1331
BstSFI CTRYAG 2 cut(s) 594, 1395
BstV1I GCAGC 4 cut(s) 484, 580, 928, 931
BstX2I RGATCY 1 cut(s) 125
BstXI CCANNNNNNTGG 1 cut(s) 455
BstYI RGATCY 1 cut(s) 125
BstZI CGGCCG 1 cut(s) 310
Bsu36I CCTNAGG 1 cut(s) 207
BsuRI GGCC 4 cut(s) 31, 312, 1090, 1383
BtgI CCRYGG 1 cut(s) 85
BtgZI GCGATG 1 cut(s) 468
BtsCI GGATG 1 cut(s) 202
BtsI GCAGTG 3 cut(s) 789, 931, 1011
BtsIMutI CAGTG 6 cut(s) 789, 931, 933, 1011, 1249, 1404
Cac8I GCNNGC 8 cut(s) 95, 105, 310, 348, 574, 832, 1329, 1437
CfoI GCGC 1 cut(s) 1181
Cfr10I RCCGGY 1 cut(s) 308
Cfr13I GGNCC 2 cut(s) 389, 434
CsiI ACCWGGT 1 cut(s) 391
Csp6I GTAC 7 cut(s) 286, 695, 817, 877, 1190, 1265, 1360
CviAII CATG 9 cut(s) 86, 486, 494, 836, 993, 1112, 1149, 1167, 1205
CviQI GTAC 7 cut(s) 286, 695, 817, 877, 1190, 1265, 1360
DdeI CTNAG 6 cut(s) 207, 273, 342, 351, 1320, 1340
DpnI GATC 5 cut(s) 127, 236, 788, 909, 1050
DpnII GATC 5 cut(s) 125, 234, 786, 907, 1048
EaeI YGGCCR 1 cut(s) 310
EagI CGGCCG 1 cut(s) 310
Eam1104I CTCTTC 3 cut(s) 57, 372, 1488
EarI CTCTTC 3 cut(s) 57, 372, 1488
EclXI CGGCCG 1 cut(s) 310
Eco130I CCWWGG 1 cut(s) 85
Eco147I AGGCCT 2 cut(s) 31, 1383
Eco24I GRGCYC 1 cut(s) 164
Eco47I GGWCC 2 cut(s) 389, 434
Eco52I CGGCCG 1 cut(s) 310
Eco81I CCTNAGG 1 cut(s) 207
Eco88I CYCGRG 1 cut(s) 425
EcoNI CCTNNNNNAGG 2 cut(s) 210, 1440
EcoO109I RGGNCCY 1 cut(s) 389
EcoRII CCWGG 4 cut(s) 247, 391, 1212, 1331
EcoT14I CCWWGG 1 cut(s) 85
EcoT22I ATGCAT 1 cut(s) 1078
EcoT38I GRGCYC 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 85
FaeI CATG 9 cut(s) 89, 489, 497, 839, 996, 1115, 1152, 1170, 1208
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FatI CATG 9 cut(s) 85, 485, 493, 835, 992, 1111, 1148, 1166, 1204
FauI CCCGC 1 cut(s) 569
FbaI TGATCA 1 cut(s) 786
FblI GTMKAC 1 cut(s) 1001
Fnu4HI GCNGC 4 cut(s) 498, 594, 942, 945
FokI GGATG 1 cut(s) 189
FriOI GRGCYC 1 cut(s) 164
Fsp4HI GCNGC 4 cut(s) 498, 594, 942, 945
FspBI CTAG 1 cut(s) 1507
GlaI GCGC 1 cut(s) 1180
GluI GCNGC 4 cut(s) 498, 594, 942, 945
GsaI CCCAGC 2 cut(s) 531, 1359
GsuI CTGGAG 1 cut(s) 1235
HaeIII GGCC 4 cut(s) 31, 312, 1090, 1383
HapII CCGG 1 cut(s) 309
HhaI GCGC 1 cut(s) 1181
Hin1II CATG 9 cut(s) 89, 489, 497, 839, 996, 1115, 1152, 1170, 1208
Hin6I GCGC 1 cut(s) 1179
HinP1I GCGC 1 cut(s) 1179
HincII GTYRAC 1 cut(s) 967
HindII GTYRAC 1 cut(s) 967
HinfI GANTC 6 cut(s) 356, 444, 490, 518, 1084, 1303
HpaII CCGG 1 cut(s) 309
HphI GGTGA 4 cut(s) 235, 354, 628, 1114
Hpy166II GTNNAC 3 cut(s) 967, 1002, 1362
Hpy188I TCNGA 3 cut(s) 443, 562, 850
Hpy188III TCNNGA 5 cut(s) 209, 638, 1136, 1292, 1487
Hpy8I GTNNAC 3 cut(s) 967, 1002, 1362
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 5 cut(s) 175, 206, 214, 220, 999
HpyCH4III ACNGT 5 cut(s) 295, 881, 898, 1189, 1399
HpyCH4IV ACGT 5 cut(s) 141, 261, 288, 963, 1095
HpyF10VI GCNNNNNNNGC 6 cut(s) 314, 347, 416, 836, 938, 1112
HpyF3I CTNAG 6 cut(s) 207, 273, 342, 351, 1320, 1340
HpySE526I ACGT 5 cut(s) 141, 261, 288, 963, 1095
Hsp92II CATG 9 cut(s) 89, 489, 497, 839, 996, 1115, 1152, 1170, 1208
HspAI GCGC 1 cut(s) 1179
KpnI GGTACC 1 cut(s) 1268
KroI GCCGGC 1 cut(s) 308
KroNI GCCGGC 1 cut(s) 310
Ksp22I TGATCA 1 cut(s) 786
Kzo9I GATC 5 cut(s) 125, 234, 786, 907, 1048
LmnI GCTCC 2 cut(s) 123, 416
Lsp1109I GCAGC 4 cut(s) 484, 580, 928, 931
LweI GCATC 5 cut(s) 559, 919, 928, 1190, 1423
MabI ACCWGGT 1 cut(s) 391
MaeI CTAG 1 cut(s) 1507
MaeII ACGT 5 cut(s) 141, 261, 288, 963, 1095
MaeIII GTNAC 4 cut(s) 289, 898, 1120, 1270
MalI GATC 5 cut(s) 127, 236, 788, 909, 1050
MboI GATC 5 cut(s) 125, 234, 786, 907, 1048
MboII GAAGA 9 cut(s) 44, 148, 170, 351, 359, 362, 393, 1298, 1475
MflI RGATCY 1 cut(s) 125
MhlI GDGCHC 1 cut(s) 164
MlyI GAGTC 1 cut(s) 527
MmeI TCCRAC 1 cut(s) 606
Mph1103I ATGCAT 1 cut(s) 1078
MroNI GCCGGC 1 cut(s) 308
MseI TTAA 2 cut(s) 240, 870
MslI CAYNNNNRTG 3 cut(s) 453, 1116, 1367
MspA1I CMGCKG 1 cut(s) 593
MspI CCGG 1 cut(s) 309
MspR9I CCNGG 4 cut(s) 249, 393, 1214, 1333
Mva1269I GAATGC 1 cut(s) 784
MvaI CCWGG 4 cut(s) 249, 393, 1214, 1333
MwoI GCNNNNNNNGC 6 cut(s) 314, 347, 416, 836, 938, 1112
NaeI GCCGGC 1 cut(s) 310
NcoI CCATGG 1 cut(s) 85
NdeII GATC 5 cut(s) 125, 234, 786, 907, 1048
NgoMIV GCCGGC 1 cut(s) 308
NlaIII CATG 9 cut(s) 89, 489, 497, 839, 996, 1115, 1152, 1170, 1208
NlaIV GGNNCC 3 cut(s) 1146, 1266, 1337
NmuCI GTSAC 3 cut(s) 898, 1120, 1270
NsiI ATGCAT 1 cut(s) 1078
OliI CACNNNNGTG 1 cut(s) 1367
PaeR7I CTCGAG 1 cut(s) 425
PceI AGGCCT 2 cut(s) 31, 1383
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PctI GAATGC 1 cut(s) 784
PdiI GCCGGC 1 cut(s) 310
PfeI GAWTC 5 cut(s) 356, 444, 490, 1084, 1303
PfoI TCCNGGA 1 cut(s) 1212
PkrI GCNGC 4 cut(s) 499, 595, 943, 946
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
PpuMI RGGWCCY 1 cut(s) 389
PshBI ATTAAT 1 cut(s) 870
PsiI TTATAA 1 cut(s) 975
Psp5II RGGWCCY 1 cut(s) 389
Psp6I CCWGG 4 cut(s) 247, 391, 1212, 1331
PspFI CCCAGC 2 cut(s) 527, 1355
PspGI CCWGG 4 cut(s) 247, 391, 1212, 1331
PspN4I GGNNCC 3 cut(s) 1146, 1266, 1337
PspPI GGNCC 2 cut(s) 389, 434
PspPPI RGGWCCY 1 cut(s) 389
PspXI VCTCGAGB 1 cut(s) 425
PstI CTGCAG 1 cut(s) 598
PsuI RGATCY 1 cut(s) 125
PvuII CAGCTG 1 cut(s) 593
RsaI GTAC 7 cut(s) 287, 696, 818, 878, 1191, 1266, 1361
RsaNI GTAC 7 cut(s) 286, 695, 817, 877, 1190, 1265, 1360
RseI CAYNNNNRTG 3 cut(s) 453, 1116, 1367
SaqAI TTAA 2 cut(s) 240, 870
SatI GCNGC 4 cut(s) 498, 594, 942, 945
Sau3AI GATC 5 cut(s) 125, 234, 786, 907, 1048
Sau96I GGNCC 2 cut(s) 389, 434
ScaI AGTACT 1 cut(s) 878
SchI GAGTC 1 cut(s) 527
ScrFI CCNGG 4 cut(s) 249, 393, 1214, 1333
SduI GDGCHC 1 cut(s) 164
SexAI ACCWGGT 1 cut(s) 391
SfaNI GCATC 5 cut(s) 559, 919, 928, 1190, 1423
SfcI CTRYAG 2 cut(s) 594, 1395
Sfr274I CTCGAG 1 cut(s) 425
SinI GGWCC 2 cut(s) 389, 434
SlaI CTCGAG 1 cut(s) 425
SmiMI CAYNNNNRTG 3 cut(s) 453, 1116, 1367
SmlI CTYRAG 3 cut(s) 425, 515, 758
SmoI CTYRAG 3 cut(s) 425, 515, 758
SseBI AGGCCT 2 cut(s) 31, 1383
SsiI CCGC 1 cut(s) 576
SspI AATATT 1 cut(s) 1038
SspMI CTAG 1 cut(s) 1507
StuI AGGCCT 2 cut(s) 31, 1383
StyD4I CCNGG 4 cut(s) 247, 391, 1212, 1331
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 5 cut(s) 295, 881, 898, 1189, 1399
TaiI ACGT 5 cut(s) 144, 264, 291, 966, 1098
TaqI TCGA 4 cut(s) 153, 426, 1228, 1374
TatI WGTACW 1 cut(s) 876
TfiI GAWTC 5 cut(s) 356, 444, 490, 1084, 1303
Tru1I TTAA 2 cut(s) 240, 870
Tru9I TTAA 2 cut(s) 240, 870
TscAI CASTG 6 cut(s) 789, 931, 940, 1018, 1249, 1404
TseFI GTSAC 3 cut(s) 898, 1120, 1270
TseI GCWGC 4 cut(s) 497, 593, 941, 944
Tsp45I GTSAC 3 cut(s) 898, 1120, 1270
TspDTI ATGAA 6 cut(s) 17, 474, 531, 810, 981, 1316
TspGWI ACGGA 1 cut(s) 1041
TspRI CASTG 6 cut(s) 789, 931, 940, 1018, 1249, 1404
VpaK11BI GGWCC 2 cut(s) 389, 434
VspI ATTAAT 1 cut(s) 870
XagI CCTNNNNNAGG 2 cut(s) 210, 1440
XapI RAATTY 1 cut(s) 1043
XhoI CTCGAG 1 cut(s) 425
XmiI GTMKAC 1 cut(s) 1001
XspI CTAG 1 cut(s) 1507
ZrmI AGTACT 1 cut(s) 878
Zsp2I ATGCAT 1 cut(s) 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.