Rh1AG032500

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0007095
Physical Location & Seq
Forward (+)
5822057 .. 5826396
4340 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG032500.1

Sequence Viewer

Length: 1248 bp
ATGAATATTCCCAAGCCTTTTTTGGTTCTTTTCTCTTCCTTTCTCATTCTTTCTTGCTTCTCCATAGCTCAAATCCATGGGAGCAAGCATTCACAAGCGCTTTCTCAGCTGTACAAGTCCAAGCTGAATTCCAAAAGCGCAGGCATCGACACGAAGCCATTCGAGGCGATTCTTCATGCGAACGCAACCAGCATTCATCCCCAAGAAGGGTTGAAGGAGAAAGATCGGATCAAAAGCTTGCCTGGACAACCCAAGGTGAAGTTCAGTCAGTATGGTGGGTATGTTACTGTGGATAAAAAAGCAGGTCGTGCACTTTTTTACTACTTTGCTGAAGCTGACAATCAGCAGGCCAAGGATTCTTTGCCTCTTCTTCTATGGCTTAATGGAGGACCTGGATGTTCCTCTCTTGCCTATGGAGCAATGCTTGAGCTCGGACCATTCCGTATCCATAGCGATGGCAAAACCCTTTACAGAAATAGATTTTCATGGAACTACGCTGCAAATGTTTTGTTCCTTGAGTCTCCTGCTGGTGTGGGTTTTTCATACTCTAACAGAACATCAGATTATGATAAAAGTGGGGACAGAAGAACAGCTGCAGATAATTATGTGTTCTTAGTGAATTGGTTGGAGAGGTTTCCTGAATATAAGGGGAGGGAGTTCTATATTGCTGGTGAAAGCTATGCAGGGCACTATGTACCTCAACTTGCACAGACTATCCTCCACCACAACAAGAAGGCTAATAGAACCATCATCAACCTCAAGGGCATAATTATTGGGAATGCAGTGATTAATGATGAAACTGATACAAGAGGAATGTATGACTACCTTGCTTCCCATGCTCTCATTTCCGATGAAATTGCAAATCAAGTCCGAAAATACTGTGATTTTTCACCCAATGCCACTATTCAACCCAAAGAGTGCGATGACGCTACTGTTGCTATTGAGAAGGCTACCTATTTCATTGATATCTATAACATCTATGCCCCCTCATGTGCCTCTTCCAATCTCACTGTCCAACCCAAAAAGGCTTCAATACTGAAATTTGATCCGTGCAGCGAGTACTATGCGGAAGCTTATTTGAATCGGCCTGATGTTCAAGAAGCTCTCCATGCTAATGTCACTAAACTCACACATGACTGGGAACCATGTAGTGATGTAATCAAACACTGGAGAGATAGCTCATCAACTATACTACCCCTTCTACATGAGTTTATGGATAATGGACTTCGAGTCTGGATTTTTAGGTAG

Protein Analysis

415

Amino Acids

46.79

Weight (kDa)

8.02

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 81 - 414 6.3e-115 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 293
AciI CCGC 1 cut(s) 1067
AclWI GGATC 2 cut(s) 236, 1040
AcsI RAATTY 2 cut(s) 127, 1040
AcuI CTGAAG 1 cut(s) 351
AfaI GTAC 3 cut(s) 113, 696, 1061
AfeI AGCGCT 1 cut(s) 99
AfiI CCNNNNNNNGG 2 cut(s) 206, 207
AgsI TTSAA 5 cut(s) 214, 908, 1032, 1081, 1097
AhdI GACNNNNNGTC 1 cut(s) 1229
AjnI CCWGG 2 cut(s) 241, 391
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
Alw21I GWGCWC 2 cut(s) 313, 432
Alw26I GTCTC 1 cut(s) 525
Alw44I GTGCAC 1 cut(s) 309
AlwI GGATC 2 cut(s) 236, 1040
Aor51HI AGCGCT 1 cut(s) 99
AoxI GGCC 2 cut(s) 348, 1085
ApaLI GTGCAC 1 cut(s) 309
ApeKI GCWGC 3 cut(s) 497, 593, 1053
ApoI RAATTY 2 cut(s) 127, 1040
AseI ATTAAT 1 cut(s) 789
Asp700I GAANNNNTTC 1 cut(s) 158
AspLEI GCGC 2 cut(s) 100, 140
AspS9I GGNCC 2 cut(s) 389, 434
AsuHPI GGTGA 3 cut(s) 268, 683, 882
AvaII GGWCC 2 cut(s) 389, 434
BaeGI GKGCMC 2 cut(s) 313, 690
BanII GRGCYC 1 cut(s) 432
Bbv12I GWGCWC 2 cut(s) 313, 432
BbvI GCAGC 3 cut(s) 484, 580, 1065
BccI CCATC 2 cut(s) 449, 755
BcgI CGANNNNNNTGC 4 cut(s) 839, 873, 1046, 1080
BciT130I CCWGG 2 cut(s) 243, 393
BciVI GTATCC 1 cut(s) 455
BcoDI GTCTC 1 cut(s) 525
BfmI CTRYAG 1 cut(s) 594
BfoI RGCGCY 1 cut(s) 101
BfuAI ACCTGC 1 cut(s) 293
BfuI GTATCC 1 cut(s) 455
BisI GCNGC 3 cut(s) 498, 594, 1054
BlsI GCNGC 3 cut(s) 499, 595, 1055
BmcAI AGTACT 1 cut(s) 1061
Bme1390I CCNGG 2 cut(s) 243, 393
Bme18I GGWCC 2 cut(s) 389, 434
BmeRI GACNNNNNGTC 1 cut(s) 1229
BmgT120I GGNCC 2 cut(s) 389, 434
BmiI GGNNCC 1 cut(s) 1143
BmrFI CCNGG 2 cut(s) 243, 393
BmrI ACTGGG 1 cut(s) 1147
BmsI GCATC 1 cut(s) 153
BmuI ACTGGG 1 cut(s) 1147
BpmI CTGGAG 1 cut(s) 1189
BpuEI CTTGAG 3 cut(s) 446, 536, 743
BsaJI CCNNGG 3 cut(s) 76, 252, 351
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 207
Bse1I ACTGG 2 cut(s) 1142, 1172
Bse3DI GCAATG 1 cut(s) 426
BseBI CCWGG 2 cut(s) 243, 393
BseDI CCNNGG 3 cut(s) 76, 252, 351
BseGI GGATG 2 cut(s) 196, 401
BseLI CCNNNNNNNGG 2 cut(s) 206, 207
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 1 cut(s) 119
BseNI ACTGG 2 cut(s) 1142, 1172
BseSI GKGCMC 2 cut(s) 313, 690
BseXI GCAGC 3 cut(s) 484, 580, 1065
BsgI GTGCAG 1 cut(s) 1072
BshFI GGCC 2 cut(s) 350, 1087
BsiHKAI GWGCWC 2 cut(s) 313, 432
BslFI GGGAC 1 cut(s) 593
BslI CCNNNNNNNGG 2 cut(s) 206, 207
BsmAI GTCTC 1 cut(s) 525
BsmFI GGGAC 1 cut(s) 593
BsmI GAATGC 3 cut(s) 88, 192, 784
BsnI GGCC 2 cut(s) 350, 1087
Bsp1286I GDGCHC 3 cut(s) 313, 432, 690
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 3 cut(s) 223, 228, 1045
Bsp19I CCATGG 1 cut(s) 76
BspACI CCGC 1 cut(s) 1067
BspANI GGCC 2 cut(s) 350, 1087
BspCNI CTCAG 1 cut(s) 118
BspLI GGNNCC 1 cut(s) 1143
BspMAI CTGCAG 1 cut(s) 598
BspMI ACCTGC 1 cut(s) 293
BspPI GGATC 2 cut(s) 236, 1040
BsrDI GCAATG 1 cut(s) 426
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 2 cut(s) 1142, 1172
BssECI CCNNGG 3 cut(s) 76, 252, 351
BssMI GATC 3 cut(s) 223, 228, 1045
BssT1I CCWWGG 3 cut(s) 76, 252, 351
Bst2UI CCWGG 2 cut(s) 243, 393
Bst4CI ACNGT 4 cut(s) 289, 881, 934, 1012
Bst6I CTCTTC 3 cut(s) 40, 372, 1003
BstAPI GCANNNNNTGC 1 cut(s) 308
BstAUI TGTACA 1 cut(s) 111
BstC8I GCNNGC 4 cut(s) 86, 142, 239, 348
BstDEI CTNAG 2 cut(s) 105, 613
BstDSI CCRYGG 1 cut(s) 76
BstF5I GGATG 2 cut(s) 196, 401
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 2 cut(s) 100, 140
BstKTI GATC 3 cut(s) 226, 231, 1048
BstMAI GTCTC 1 cut(s) 525
BstMBI GATC 3 cut(s) 223, 228, 1045
BstMWI GCNNNNNNNGC 6 cut(s) 106, 308, 416, 836, 935, 1109
BstNI CCWGG 2 cut(s) 243, 393
BstSCI CCNGG 2 cut(s) 241, 391
BstSFI CTRYAG 1 cut(s) 594
BstSLI GKGCMC 2 cut(s) 313, 690
BstV1I GCAGC 3 cut(s) 484, 580, 1065
BstXI CCANNNNNNTGG 1 cut(s) 455
BsuI GTATCC 1 cut(s) 455
BsuRI GGCC 2 cut(s) 350, 1087
BtgI CCRYGG 1 cut(s) 76
BtgZI GCGATG 2 cut(s) 468, 936
BtsCI GGATG 2 cut(s) 196, 401
BtsI GCAGTG 1 cut(s) 789
BtsIMutI CAGTG 3 cut(s) 789, 1008, 1165
BveI ACCTGC 1 cut(s) 293
Cac8I GCNNGC 4 cut(s) 86, 142, 239, 348
CfoI GCGC 2 cut(s) 100, 140
Cfr13I GGNCC 2 cut(s) 389, 434
CseI GACGC 1 cut(s) 935
Csp6I GTAC 3 cut(s) 112, 695, 1060
CviAII CATG 9 cut(s) 77, 176, 486, 836, 990, 1109, 1133, 1146, 1205
CviQI GTAC 3 cut(s) 112, 695, 1060
DdeI CTNAG 2 cut(s) 105, 613
DpnI GATC 3 cut(s) 225, 230, 1047
DpnII GATC 3 cut(s) 223, 228, 1045
DriI GACNNNNNGTC 1 cut(s) 1229
Eam1104I CTCTTC 3 cut(s) 40, 372, 1003
Eam1105I GACNNNNNGTC 1 cut(s) 1229
EarI CTCTTC 3 cut(s) 40, 372, 1003
Ecl136II GAGCTC 1 cut(s) 430
Eco130I CCWWGG 3 cut(s) 76, 252, 351
Eco24I GRGCYC 1 cut(s) 432
Eco32I GATATC 1 cut(s) 967
Eco47I GGWCC 2 cut(s) 389, 434
Eco47III AGCGCT 1 cut(s) 99
Eco53kI GAGCTC 1 cut(s) 430
Eco57I CTGAAG 1 cut(s) 351
EcoICRI GAGCTC 1 cut(s) 430
EcoO109I RGGNCCY 1 cut(s) 389
EcoRI GAATTC 1 cut(s) 127
EcoRII CCWGG 2 cut(s) 241, 391
EcoRV GATATC 1 cut(s) 967
EcoT14I CCWWGG 3 cut(s) 76, 252, 351
EcoT38I GRGCYC 1 cut(s) 432
ErhI CCWWGG 3 cut(s) 76, 252, 351
FaeI CATG 9 cut(s) 80, 179, 489, 839, 993, 1112, 1136, 1149, 1208
FaqI GGGAC 1 cut(s) 593
FatI CATG 9 cut(s) 76, 175, 485, 835, 989, 1108, 1132, 1145, 1204
Fnu4HI GCNGC 3 cut(s) 498, 594, 1054
FokI GGATG 2 cut(s) 183, 408
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 3 cut(s) 498, 594, 1054
GlaI GCGC 2 cut(s) 99, 139
GluI GCNGC 3 cut(s) 498, 594, 1054
GsuI CTGGAG 1 cut(s) 1189
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 2 cut(s) 350, 1087
HgaI GACGC 1 cut(s) 935
HhaI GCGC 2 cut(s) 100, 140
Hin1II CATG 9 cut(s) 80, 179, 489, 839, 993, 1112, 1136, 1149, 1208
Hin6I GCGC 2 cut(s) 98, 138
HinP1I GCGC 2 cut(s) 98, 138
HindIII AAGCTT 2 cut(s) 235, 1071
HinfI GANTC 5 cut(s) 169, 356, 518, 1081, 1230
HphI GGTGA 3 cut(s) 268, 683, 882
Hpy166II GTNNAC 1 cut(s) 311
Hpy188I TCNGA 5 cut(s) 228, 434, 562, 850, 872
Hpy188III TCNNGA 3 cut(s) 638, 1097, 1234
Hpy8I GTNNAC 1 cut(s) 311
HpyAV CCTTC 5 cut(s) 200, 208, 727, 940, 1208
HpyCH4III ACNGT 4 cut(s) 289, 881, 934, 1012
HpyCH4V TGCA 8 cut(s) 311, 500, 596, 683, 707, 782, 860, 1053
HpyF10VI GCNNNNNNNGC 6 cut(s) 106, 308, 416, 836, 935, 1109
HpyF3I CTNAG 2 cut(s) 105, 613
Hsp92II CATG 9 cut(s) 80, 179, 489, 839, 993, 1112, 1136, 1149, 1208
HspAI GCGC 2 cut(s) 98, 138
Kzo9I GATC 3 cut(s) 223, 228, 1045
LmnI GCTCC 2 cut(s) 81, 416
Lsp1109I GCAGC 3 cut(s) 484, 580, 1065
LweI GCATC 1 cut(s) 153
MaeIII GTNAC 2 cut(s) 283, 1117
MalI GATC 3 cut(s) 225, 230, 1047
MboI GATC 3 cut(s) 223, 228, 1045
MboII GAAGA 6 cut(s) 27, 164, 359, 362, 597, 990
MhlI GDGCHC 3 cut(s) 313, 432, 690
MluCI AATT 6 cut(s) 127, 601, 619, 768, 855, 1040
MlyI GAGTC 2 cut(s) 527, 1239
MmeI TCCRAC 2 cut(s) 606, 1039
MroXI GAANNNNTTC 1 cut(s) 158
MseI TTAA 2 cut(s) 381, 789
MslI CAYNNNNRTG 2 cut(s) 453, 1113
MspA1I CMGCKG 2 cut(s) 109, 593
MspR9I CCNGG 2 cut(s) 243, 393
Mva1269I GAATGC 3 cut(s) 88, 192, 784
MvaI CCWGG 2 cut(s) 243, 393
MwoI GCNNNNNNNGC 6 cut(s) 106, 308, 416, 836, 935, 1109
NcoI CCATGG 1 cut(s) 76
NdeII GATC 3 cut(s) 223, 228, 1045
NlaIII CATG 9 cut(s) 80, 179, 489, 839, 993, 1112, 1136, 1149, 1208
NlaIV GGNNCC 1 cut(s) 1143
NmuCI GTSAC 1 cut(s) 1117
PctI GAATGC 3 cut(s) 88, 192, 784
PdmI GAANNNNTTC 1 cut(s) 158
PfeI GAWTC 3 cut(s) 169, 356, 1081
PkrI GCNGC 3 cut(s) 499, 595, 1055
PleI GAGTC 2 cut(s) 526, 1238
PpsI GAGTC 2 cut(s) 526, 1238
PpuMI RGGWCCY 1 cut(s) 389
PshBI ATTAAT 1 cut(s) 789
Psp124BI GAGCTC 1 cut(s) 432
Psp5II RGGWCCY 1 cut(s) 389
Psp6I CCWGG 2 cut(s) 241, 391
PspGI CCWGG 2 cut(s) 241, 391
PspN4I GGNNCC 1 cut(s) 1143
PspPI GGNCC 2 cut(s) 389, 434
PspPPI RGGWCCY 1 cut(s) 389
PstI CTGCAG 1 cut(s) 598
PvuII CAGCTG 2 cut(s) 109, 593
RsaI GTAC 3 cut(s) 113, 696, 1061
RsaNI GTAC 3 cut(s) 112, 695, 1060
RseI CAYNNNNRTG 2 cut(s) 453, 1113
SacI GAGCTC 1 cut(s) 432
SaqAI TTAA 2 cut(s) 381, 789
SatI GCNGC 3 cut(s) 498, 594, 1054
Sau3AI GATC 3 cut(s) 223, 228, 1045
Sau96I GGNCC 2 cut(s) 389, 434
ScaI AGTACT 1 cut(s) 1061
SchI GAGTC 2 cut(s) 527, 1239
ScrFI CCNGG 2 cut(s) 243, 393
SduI GDGCHC 3 cut(s) 313, 432, 690
SfaNI GCATC 1 cut(s) 153
SfcI CTRYAG 1 cut(s) 594
SinI GGWCC 2 cut(s) 389, 434
SmiMI CAYNNNNRTG 2 cut(s) 453, 1113
SmlI CTYRAG 3 cut(s) 425, 515, 758
SmoI CTYRAG 3 cut(s) 425, 515, 758
Sse9I AATT 6 cut(s) 127, 601, 619, 768, 855, 1040
SsiI CCGC 1 cut(s) 1067
SspI AATATT 1 cut(s) 7
SstI GAGCTC 1 cut(s) 432
StyD4I CCNGG 2 cut(s) 241, 391
StyI CCWWGG 3 cut(s) 76, 252, 351
TaaI ACNGT 4 cut(s) 289, 881, 934, 1012
TaqI TCGA 3 cut(s) 147, 162, 1228
TasI AATT 6 cut(s) 127, 601, 619, 768, 855, 1040
TatI WGTACW 2 cut(s) 111, 1059
TfiI GAWTC 3 cut(s) 169, 356, 1081
Tru1I TTAA 2 cut(s) 381, 789
Tru9I TTAA 2 cut(s) 381, 789
TscAI CASTG 3 cut(s) 789, 1015, 1172
TseFI GTSAC 1 cut(s) 1117
TseI GCWGC 3 cut(s) 497, 593, 1053
Tsp45I GTSAC 1 cut(s) 1117
TspDTI ATGAA 8 cut(s) 17, 164, 185, 474, 531, 810, 867, 949
TspGWI ACGGA 2 cut(s) 431, 1038
TspRI CASTG 3 cut(s) 789, 1015, 1172
VneI GTGCAC 1 cut(s) 309
VpaK11BI GGWCC 2 cut(s) 389, 434
VspI ATTAAT 1 cut(s) 789
XapI RAATTY 2 cut(s) 127, 1040
XcmI CCANNNNNNNNNTGG 1 cut(s) 19
XmnI GAANNNNTTC 1 cut(s) 158
ZrmI AGTACT 1 cut(s) 1061
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.