MD02G1300900.v1.1

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007095
Physical Location & Seq
Reverse (-)
35558207 .. 35564746
6540 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1300900.v1.1.491

Sequence Viewer

Length: 1509 bp
ATGAATACAATCAAAATGCAAACAATTAGGCCTTGTTTGGCACTTTTCTCTCTTCTCGTTCTTTCTTGCTTCGTAGCTCAAATCCATGGAAGCAAGCAATCAGAAGCACTTTCACGGCTCTACAAGTCCAAATTGAAGAACGCCGCCCCCATCGACAAGAGCCCTTTCGTGGCGATTTTTCACGCAAACACAACAAAAACTCATACTCAGGAGGGATTGAAGGAGAAGGATAGGATCGATAAGTTGCCTGGACAACCCAAAGTGAAATTCTCTCAGTATGGCGGGTACGTTACGGTGGATAAAAAAGCCGGTCGTGCACTATTTTACTACTTAGTTGAAGCTGAGAAAGCTAAGGATTCTTCACCTCTTCTTCTATGGCTCAATGGAGGACCTGGTTGTTCTTCTCTTGCCTATGGAGCAATGCTCGAGCTTGGACCGTTCCGAATCCACAGCGATGGAAAAACACTTTACAGAAACAGATTTTCATGGAATTATGCTGCCAATGTGTTGTTCCTTGAGTCTCCTGCTGGGGTGGGGTTTTCTTACTCAAATAGAACATCTGATTACGATGCAAGCGGGGATAGAAACACAGCTGCAGATAATTATGTATTTTTGGTGAACTGGTTGGAGAGGTTTCCTGAATACAAGGGCAGGGAGTTTTATATTTCTGGGGAAAGCTATGCAGGGCATTATGTACCTCAACTTGCACATACCATTCTCAACCATAATAAAAGGGCAAACAAGACCATTATCAACCTCAAGGGCATTATAATTGGGAATGCAGTGATCAATGATGAAACTGATTCAAAAGGAATGTACGACTACCTTGCGAGCCATGCTCTGATTTCAGATAAAACTGCCAACAATATAAACAAGTACTGTAACTTTGCAGCTAATGCCACCACTCAACCTAAGGAGTGCAATGATGCCACTGATGCTGCAAATAAAGATACCTACTACGTCGATATTTATAACATCTATGCCCCTTTATGCTTGTCTACGAACCTCACTGCAAAACCCAAAAGGACTTCAATTTTCGAATTTGATCCGTGTAGTGAGTACTATGCATATGGTTATTTGAATCGTCCTGACGTCCAACAAGCTCTCCATGCCAATGTCACCAAACTGTCACACGACTGGGAACCATGTAGTGATATAATCACACACTGGGGCGATAGCGCATCAACCGTACTACCTCTTTTACATGAGTTCCTGGATAATGGACTTCGAGTGTGGATTTTCAGTGGCGACATTGATGGGAGGGTACCAGTGACTTCAACAAAGTATTCTATTGACAAGATGAATCTTGCAGTAAAGACTGAGTGGCATGCTTGGTTCCTTAGTGGAGAGGTTGGTGGGTACACACAAGTGTACGAGGGAGGCCTAACATTTGCTACAGTGAGAGAGGCAGGGCATCAAGTTCCAAGCTATCAGCCTGCAAGAGGACTTTCTCTCATCAAGCACTTCCTTGATGGCACACCTCTTCCTGATACTACAAGATATAACTAA

Protein Analysis

503

Amino Acids

56.17

Weight (kDa)

7.96

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 83 - 492 2.6e-144 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 770, 972
AatII GACGTC 1 cut(s) 1095
Acc65I GGTACC 1 cut(s) 1264
AccB1I GGYRCC 1 cut(s) 1264
AccI GTMKAC 1 cut(s) 998
AciI CCGC 3 cut(s) 144, 282, 576
AclWI GGATC 2 cut(s) 242, 1040
AcsI RAATTY 2 cut(s) 266, 1040
AcyI GRCGYC 1 cut(s) 1092
AfaI GTAC 9 cut(s) 287, 696, 818, 878, 1061, 1191, 1266, 1361, 1373
AfiI CCNNNNNNNGG 2 cut(s) 169, 1442
AgsI TTSAA 7 cut(s) 136, 220, 338, 807, 1032, 1081, 1278
AjnI CCWGG 3 cut(s) 247, 391, 1212
AjuI GAANNNNNNNTTGG 4 cut(s) 251, 283, 494, 526
AleI CACNNNNGTG 1 cut(s) 1367
AluBI AGCT 9 cut(s) 77, 341, 350, 430, 593, 678, 893, 1103, 1428
AluI AGCT 9 cut(s) 77, 341, 350, 430, 593, 678, 893, 1103, 1428
Alw21I GWGCWC 1 cut(s) 319
Alw26I GTCTC 1 cut(s) 525
Alw44I GTGCAC 1 cut(s) 315
AlwI GGATC 2 cut(s) 242, 1040
Ama87I CYCGRG 1 cut(s) 425
AoxI GGCC 2 cut(s) 29, 1381
ApaLI GTGCAC 1 cut(s) 315
ApeKI GCWGC 4 cut(s) 497, 593, 890, 938
ApoI RAATTY 2 cut(s) 266, 1040
Asp718I GGTACC 1 cut(s) 1264
AspLEI GCGC 1 cut(s) 1181
AspS9I GGNCC 2 cut(s) 389, 434
AsuHPI GGTGA 3 cut(s) 354, 628, 1111
AsuII TTCGAA 1 cut(s) 1038
AvaI CYCGRG 1 cut(s) 425
AvaII GGWCC 2 cut(s) 389, 434
AxyI CCTNAGG 1 cut(s) 912
BaeGI GKGCMC 1 cut(s) 319
BanI GGYRCC 1 cut(s) 1264
BanII GRGCYC 1 cut(s) 164
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 4 cut(s) 484, 580, 902, 925
BccI CCATC 4 cut(s) 158, 449, 1250, 1466
BceAI ACGGC 1 cut(s) 131
BcgI CGANNNNNNTGC 2 cut(s) 809, 843
BciT130I CCWGG 3 cut(s) 249, 393, 1214
BclI TGATCA 1 cut(s) 786
BcoDI GTCTC 1 cut(s) 525
BfmI CTRYAG 2 cut(s) 594, 1395
BisI GCNGC 5 cut(s) 144, 498, 594, 891, 939
BlsI GCNGC 5 cut(s) 145, 499, 595, 892, 940
BmcAI AGTACT 2 cut(s) 878, 1061
Bme1390I CCNGG 3 cut(s) 249, 393, 1214
Bme18I GGWCC 2 cut(s) 389, 434
BmeT110I CYCGRG 1 cut(s) 425
BmgT120I GGNCC 2 cut(s) 389, 434
BmiI GGNNCC 3 cut(s) 1143, 1266, 1337
BmrFI CCNGG 3 cut(s) 249, 393, 1214
BmrI ACTGGG 2 cut(s) 1147, 1177
BmsI GCATC 5 cut(s) 559, 916, 925, 1190, 1423
BmuI ACTGGG 2 cut(s) 1147, 1177
BplI GAGNNNNNCTC 4 cut(s) 408, 440, 823, 855
Bpu10I CCTNAGC 1 cut(s) 351
Bpu14I TTCGAA 1 cut(s) 1038
BpuEI CTTGAG 2 cut(s) 536, 743
Bsa29I ATCGAT 1 cut(s) 237
BsaHI GRCGYC 1 cut(s) 1092
BsaJI CCNNGG 1 cut(s) 85
BsaXI ACNNNNNCTCC 2 cut(s) 1089, 1119
Bsc4I CCNNNNNNNGG 2 cut(s) 169, 1442
Bse118I RCCGGY 1 cut(s) 308
Bse1I ACTGG 4 cut(s) 626, 1142, 1172, 1268
Bse21I CCTNAGG 1 cut(s) 912
Bse3DI GCAATG 2 cut(s) 426, 928
BseBI CCWGG 3 cut(s) 249, 393, 1214
BseCI ATCGAT 1 cut(s) 237
BseDI CCNNGG 1 cut(s) 85
BseLI CCNNNNNNNGG 2 cut(s) 169, 1442
BseMI GCAATG 2 cut(s) 426, 928
BseMII CTCAG 4 cut(s) 221, 287, 333, 1311
BseNI ACTGG 4 cut(s) 626, 1142, 1172, 1268
BseSI GKGCMC 1 cut(s) 319
BseXI GCAGC 4 cut(s) 484, 580, 902, 925
BseYI CCCAGC 1 cut(s) 527
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 2 cut(s) 31, 1383
BshNI GGYRCC 1 cut(s) 1264
BshVI ATCGAT 1 cut(s) 237
BsiEI CGRYCG 1 cut(s) 313
BsiHKAI GWGCWC 1 cut(s) 319
BsiHKCI CYCGRG 1 cut(s) 425
BsiSI CCGG 1 cut(s) 309
BslI CCNNNNNNNGG 2 cut(s) 169, 1442
BsmAI GTCTC 1 cut(s) 525
BsmI GAATGC 1 cut(s) 784
BsnI GGCC 2 cut(s) 31, 1383
BsoBI CYCGRG 1 cut(s) 425
Bsp119I TTCGAA 1 cut(s) 1038
Bsp1286I GDGCHC 2 cut(s) 164, 319
Bsp143I GATC 3 cut(s) 234, 786, 1045
Bsp19I CCATGG 1 cut(s) 85
BspACI CCGC 3 cut(s) 144, 282, 576
BspANI GGCC 2 cut(s) 31, 1383
BspCNI CTCAG 4 cut(s) 220, 286, 334, 1312
BspDI ATCGAT 1 cut(s) 237
BspLI GGNNCC 3 cut(s) 1143, 1266, 1337
BspMAI CTGCAG 1 cut(s) 598
BspPI GGATC 2 cut(s) 242, 1040
BspT104I TTCGAA 1 cut(s) 1038
BspT107I GGYRCC 1 cut(s) 1264
BsrDI GCAATG 2 cut(s) 426, 928
BsrFI RCCGGY 1 cut(s) 308
BsrI ACTGG 4 cut(s) 626, 1142, 1172, 1268
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 1 cut(s) 85
BssMI GATC 3 cut(s) 234, 786, 1045
BssNI GRCGYC 1 cut(s) 1092
BssT1I CCWWGG 1 cut(s) 85
Bst2UI CCWGG 3 cut(s) 249, 393, 1214
Bst4CI ACNGT 6 cut(s) 295, 438, 881, 1128, 1189, 1399
Bst6I CTCTTC 3 cut(s) 57, 372, 1488
BstACI GRCGYC 1 cut(s) 1092
BstAPI GCANNNNNTGC 1 cut(s) 896
BstBI TTCGAA 1 cut(s) 1038
BstC8I GCNNGC 5 cut(s) 95, 574, 832, 1329, 1437
BstDEI CTNAG 8 cut(s) 207, 273, 331, 342, 351, 912, 1320, 1340
BstDSI CCRYGG 1 cut(s) 85
BstENI CCTNNNNNAGG 1 cut(s) 1440
BstHHI GCGC 1 cut(s) 1181
BstKTI GATC 3 cut(s) 237, 789, 1048
BstMAI GTCTC 1 cut(s) 525
BstMBI GATC 3 cut(s) 234, 786, 1045
BstMCI CGRYCG 1 cut(s) 313
BstMWI GCNNNNNNNGC 7 cut(s) 314, 347, 416, 836, 896, 935, 1109
BstNI CCWGG 3 cut(s) 249, 393, 1214
BstNSI RCATGY 1 cut(s) 1331
BstSCI CCNGG 3 cut(s) 247, 391, 1212
BstSFI CTRYAG 2 cut(s) 594, 1395
BstSLI GKGCMC 1 cut(s) 319
BstV1I GCAGC 4 cut(s) 484, 580, 902, 925
BstXI CCANNNNNNTGG 1 cut(s) 455
Bsu15I ATCGAT 1 cut(s) 237
Bsu36I CCTNAGG 1 cut(s) 912
BsuRI GGCC 2 cut(s) 31, 1383
BsuTUI ATCGAT 1 cut(s) 237
BtgI CCRYGG 1 cut(s) 85
BtgZI GCGATG 1 cut(s) 468
BtsI GCAGTG 2 cut(s) 789, 1008
BtsIMutI CAGTG 7 cut(s) 789, 930, 1008, 1165, 1249, 1275, 1404
Cac8I GCNNGC 5 cut(s) 95, 574, 832, 1329, 1437
CfoI GCGC 1 cut(s) 1181
Cfr10I RCCGGY 1 cut(s) 308
Cfr13I GGNCC 2 cut(s) 389, 434
ClaI ATCGAT 1 cut(s) 237
CsiI ACCWGGT 1 cut(s) 391
Csp6I GTAC 9 cut(s) 286, 695, 817, 877, 1060, 1190, 1265, 1360, 1372
CviAII CATG 7 cut(s) 86, 486, 836, 1109, 1146, 1205, 1328
CviQI GTAC 9 cut(s) 286, 695, 817, 877, 1060, 1190, 1265, 1360, 1372
DdeI CTNAG 8 cut(s) 207, 273, 331, 342, 351, 912, 1320, 1340
DpnI GATC 3 cut(s) 236, 788, 1047
DpnII GATC 3 cut(s) 234, 786, 1045
Eam1104I CTCTTC 3 cut(s) 57, 372, 1488
EarI CTCTTC 3 cut(s) 57, 372, 1488
Eco130I CCWWGG 1 cut(s) 85
Eco147I AGGCCT 2 cut(s) 31, 1383
Eco24I GRGCYC 1 cut(s) 164
Eco47I GGWCC 2 cut(s) 389, 434
Eco81I CCTNAGG 1 cut(s) 912
Eco88I CYCGRG 1 cut(s) 425
EcoNI CCTNNNNNAGG 1 cut(s) 1440
EcoO109I RGGNCCY 1 cut(s) 389
EcoRII CCWGG 3 cut(s) 247, 391, 1212
EcoT14I CCWWGG 1 cut(s) 85
EcoT22I ATGCAT 1 cut(s) 1069
EcoT38I GRGCYC 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 85
FaeI CATG 7 cut(s) 89, 489, 839, 1112, 1149, 1208, 1331
FatI CATG 7 cut(s) 85, 485, 835, 1108, 1145, 1204, 1327
FauI CCCGC 2 cut(s) 275, 569
FauNDI CATATG 1 cut(s) 1069
FbaI TGATCA 1 cut(s) 786
FblI GTMKAC 1 cut(s) 998
Fnu4HI GCNGC 5 cut(s) 144, 498, 594, 891, 939
FriOI GRGCYC 1 cut(s) 164
Fsp4HI GCNGC 5 cut(s) 144, 498, 594, 891, 939
GlaI GCGC 1 cut(s) 1180
GluI GCNGC 5 cut(s) 144, 498, 594, 891, 939
GsaI CCCAGC 1 cut(s) 531
HaeIII GGCC 2 cut(s) 31, 1383
HapII CCGG 1 cut(s) 309
HhaI GCGC 1 cut(s) 1181
Hin1I GRCGYC 1 cut(s) 1092
Hin1II CATG 7 cut(s) 89, 489, 839, 1112, 1149, 1208, 1331
Hin6I GCGC 1 cut(s) 1179
HinP1I GCGC 1 cut(s) 1179
HinfI GANTC 6 cut(s) 356, 444, 518, 803, 1081, 1303
HpaII CCGG 1 cut(s) 309
HphI GGTGA 3 cut(s) 354, 628, 1111
Hpy166II GTNNAC 5 cut(s) 317, 619, 999, 1362, 1372
Hpy188I TCNGA 5 cut(s) 103, 443, 562, 843, 850
Hpy188III TCNNGA 4 cut(s) 209, 638, 1088, 1487
Hpy8I GTNNAC 5 cut(s) 317, 619, 999, 1362, 1372
Hpy99I CGWCG 1 cut(s) 965
HpyAV CCTTC 2 cut(s) 214, 220
HpyCH4III ACNGT 6 cut(s) 295, 438, 881, 1128, 1189, 1399
HpyCH4IV ACGT 3 cut(s) 288, 960, 1092
HpyF10VI GCNNNNNNNGC 7 cut(s) 314, 347, 416, 836, 896, 935, 1109
HpyF3I CTNAG 8 cut(s) 207, 273, 331, 342, 351, 912, 1320, 1340
HpySE526I ACGT 3 cut(s) 288, 960, 1092
Hsp92I GRCGYC 1 cut(s) 1092
Hsp92II CATG 7 cut(s) 89, 489, 839, 1112, 1149, 1208, 1331
HspAI GCGC 1 cut(s) 1179
KpnI GGTACC 1 cut(s) 1268
Ksp22I TGATCA 1 cut(s) 786
Kzo9I GATC 3 cut(s) 234, 786, 1045
LmnI GCTCC 1 cut(s) 416
Lsp1109I GCAGC 4 cut(s) 484, 580, 902, 925
LweI GCATC 5 cut(s) 559, 916, 925, 1190, 1423
MabI ACCWGGT 1 cut(s) 391
MaeII ACGT 3 cut(s) 288, 960, 1092
MaeIII GTNAC 5 cut(s) 289, 881, 1117, 1128, 1270
MalI GATC 3 cut(s) 236, 788, 1047
MboI GATC 3 cut(s) 234, 786, 1045
MboII GAAGA 7 cut(s) 44, 148, 351, 359, 362, 393, 1475
MhlI GDGCHC 2 cut(s) 164, 319
MluCI AATT 8 cut(s) 24, 131, 266, 490, 601, 771, 1032, 1040
MlyI GAGTC 1 cut(s) 527
MmeI TCCRAC 2 cut(s) 606, 1120
Mph1103I ATGCAT 1 cut(s) 1069
MslI CAYNNNNRTG 3 cut(s) 453, 1113, 1367
MspA1I CMGCKG 1 cut(s) 593
MspI CCGG 1 cut(s) 309
MspR9I CCNGG 3 cut(s) 249, 393, 1214
Mva1269I GAATGC 1 cut(s) 784
MvaI CCWGG 3 cut(s) 249, 393, 1214
MwoI GCNNNNNNNGC 7 cut(s) 314, 347, 416, 836, 896, 935, 1109
NcoI CCATGG 1 cut(s) 85
NdeI CATATG 1 cut(s) 1069
NdeII GATC 3 cut(s) 234, 786, 1045
NlaIII CATG 7 cut(s) 89, 489, 839, 1112, 1149, 1208, 1331
NlaIV GGNNCC 3 cut(s) 1143, 1266, 1337
NmuCI GTSAC 3 cut(s) 1117, 1128, 1270
NsiI ATGCAT 1 cut(s) 1069
NspI RCATGY 1 cut(s) 1331
NspV TTCGAA 1 cut(s) 1038
OliI CACNNNNGTG 1 cut(s) 1367
PaeI GCATGC 1 cut(s) 1331
PaeR7I CTCGAG 1 cut(s) 425
PceI AGGCCT 2 cut(s) 31, 1383
PctI GAATGC 1 cut(s) 784
PfeI GAWTC 5 cut(s) 356, 444, 803, 1081, 1303
PfoI TCCNGGA 1 cut(s) 1212
PkrI GCNGC 5 cut(s) 145, 499, 595, 892, 940
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
PpuMI RGGWCCY 1 cut(s) 389
PsiI TTATAA 2 cut(s) 770, 972
Psp5II RGGWCCY 1 cut(s) 389
Psp6I CCWGG 3 cut(s) 247, 391, 1212
PspFI CCCAGC 1 cut(s) 527
PspGI CCWGG 3 cut(s) 247, 391, 1212
PspN4I GGNNCC 3 cut(s) 1143, 1266, 1337
PspPI GGNCC 2 cut(s) 389, 434
PspPPI RGGWCCY 1 cut(s) 389
PspXI VCTCGAGB 1 cut(s) 425
PstI CTGCAG 1 cut(s) 598
PvuII CAGCTG 1 cut(s) 593
RsaI GTAC 9 cut(s) 287, 696, 818, 878, 1061, 1191, 1266, 1361, 1373
RsaNI GTAC 9 cut(s) 286, 695, 817, 877, 1060, 1190, 1265, 1360, 1372
RseI CAYNNNNRTG 3 cut(s) 453, 1113, 1367
SatI GCNGC 5 cut(s) 144, 498, 594, 891, 939
Sau3AI GATC 3 cut(s) 234, 786, 1045
Sau96I GGNCC 2 cut(s) 389, 434
ScaI AGTACT 2 cut(s) 878, 1061
SchI GAGTC 1 cut(s) 527
ScrFI CCNGG 3 cut(s) 249, 393, 1214
SduI GDGCHC 2 cut(s) 164, 319
SexAI ACCWGGT 1 cut(s) 391
SfaNI GCATC 5 cut(s) 559, 916, 925, 1190, 1423
SfcI CTRYAG 2 cut(s) 594, 1395
Sfr274I CTCGAG 1 cut(s) 425
SfuI TTCGAA 1 cut(s) 1038
SinI GGWCC 2 cut(s) 389, 434
SlaI CTCGAG 1 cut(s) 425
SmiMI CAYNNNNRTG 3 cut(s) 453, 1113, 1367
SmlI CTYRAG 3 cut(s) 425, 515, 758
SmoI CTYRAG 3 cut(s) 425, 515, 758
SphI GCATGC 1 cut(s) 1331
Sse9I AATT 8 cut(s) 24, 131, 266, 490, 601, 771, 1032, 1040
SseBI AGGCCT 2 cut(s) 31, 1383
SsiI CCGC 3 cut(s) 144, 282, 576
StuI AGGCCT 2 cut(s) 31, 1383
StyD4I CCNGG 3 cut(s) 247, 391, 1212
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 6 cut(s) 295, 438, 881, 1128, 1189, 1399
TaiI ACGT 3 cut(s) 291, 963, 1095
TaqI TCGA 6 cut(s) 153, 237, 426, 963, 1038, 1228
TasI AATT 8 cut(s) 24, 131, 266, 490, 601, 771, 1032, 1040
TatI WGTACW 2 cut(s) 876, 1059
TauI GCSGC 1 cut(s) 146
TfiI GAWTC 5 cut(s) 356, 444, 803, 1081, 1303
TscAI CASTG 7 cut(s) 789, 937, 1015, 1172, 1249, 1275, 1404
TseFI GTSAC 3 cut(s) 1117, 1128, 1270
TseI GCWGC 4 cut(s) 497, 593, 890, 938
Tsp45I GTSAC 3 cut(s) 1117, 1128, 1270
TspDTI ATGAA 4 cut(s) 17, 474, 810, 1316
TspGWI ACGGA 1 cut(s) 1038
TspRI CASTG 7 cut(s) 789, 937, 1015, 1172, 1249, 1275, 1404
VneI GTGCAC 1 cut(s) 315
VpaK11BI GGWCC 2 cut(s) 389, 434
XagI CCTNNNNNAGG 1 cut(s) 1440
XapI RAATTY 2 cut(s) 266, 1040
XceI RCATGY 1 cut(s) 1331
XhoI CTCGAG 1 cut(s) 425
XmiI GTMKAC 1 cut(s) 998
ZraI GACGTC 1 cut(s) 1093
ZrmI AGTACT 2 cut(s) 878, 1061
Zsp2I ATGCAT 1 cut(s) 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.