pycom07g01750

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007095
Physical Location & Seq
Forward (+)
1376346 .. 1380666
4321 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g01750.1

Sequence Viewer

Length: 1509 bp
ATGAATACAATCAAAATGCAAATAATTAGGCATTGTTCGGCAATTTTATCTCTTCTCATTCTTTCTTGCTTCGCAACTCAAATCCATGGAAGCAAGCAATCGCAAGCACTTTCACAGCTCCACAGGTCCAAATTGAAGAACGTCGCCCGCATCGACAAGAGCCCCTTCGTGGCGATTCTTCACGCAAACGCAACCAAAACTCATCCTCAGGAAGGGTTGAAGGTGAAGGATAGGATCGTTAAATTGCCTGGACAACCCAACGTGAAGTTCTCCCAGTATGGTGGGTACGTTACGGTGGATAAAAAAGCCGGCCGTGCAATGTTTTACTATTTAGTTGAAGCTGAGCAAGCTAAGGATTCTTCACCTCTTCTTCTGTGGCTCAATGGAGGACCTGGTTGTTCTTCTCTTGCCTATGGAGCAATGCTCGAGCTTGGACCGTTCCGAATCCACAGCGATGGAAAAACACTTTACAGAAACAGATTTTCATGGAATCATGCTGCTAATGTGTTGTTCCTTGAGTCTCCTGCTGGGGTGGGATTTTCATACTCAAATAGAACATCTGATTACGATGCGAGCGGGGATAGAAACACAGCAGCAGATAATTATGTATTTTTGGTGAATTGGTTGGAGAGGTTTCCTGAATACAAGGGCAGGGAGTTTTATATCTCTGGGGAAAGTTATGCAGGGCATTATGTACCTCAACTTGCACATACCATTCTATACCATAATAAGAGGGCTAACAAGACCATTATCAACCTCAAGGGCATAATAATTGGGAATGCAGTGATCAATGATGAAACTGATACAAAGGGAATGTACGACTATCTTGCGAGCCATGCTATCATTTCAGATAAAACTGCAAACGTTATTAACAACTACTGTAATTTTGCACCTAATGTCAAGACTGATCAGCGTAAGGAGTGCAATGATGCCACAGATGATGCTGCTAAAGATACCTATTACGTTGACATTTATAACATCTATGCCCCTTCATGTTTGTCTACCAACCTCACTGCAAAACCCAAAAGGACTTCGATATTCAAATTTGATCCGTGCAGTGAGGACTATGTGTATGCTTATTTGAATCGGCCTGACGTTCAACAAGCTCTCCATGCCAATGTCACCAAACTCTCACACGATTGGGAACCATGTAGTGATGTTATCACATGGGGCGATAGTGCATCAACCGTACTACCTCTTTTGCATGAGTTCCTGGAGAATGGACTTCGAGTGTGGATTTTCAGTGGCGACATTGATGGGAGGGTACCAGTGACTTCAACAAAGTATTCTCTTGACAAGATGAATCTTCCAGTAAAGACTGAGTGGCACGCTTGGTTCCTTAGTGGAGAGGTTGCTGGGTACACACAAGAGTTCGAGGGAGGCCTAACATTTGCTACAGTGAGACAGGCAGGGCATCAAGTTCCAAGCTATCAGCCTGCAAGAGGACTTTCACTCATCAAGCACTTCCTTGATGGCACACCTCTTCCTGATACCAAAAGATATAACTAG

Protein Analysis

503

Amino Acids

56.3

Weight (kDa)

8.4

Isoelectric Point (pI)

37.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 83 - 492 4.1e-144 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 975
Acc65I GGTACC 1 cut(s) 1264
AccB1I GGYRCC 1 cut(s) 1264
AccBSI CCGCTC 1 cut(s) 576
AccI GTMKAC 1 cut(s) 1001
AciI CCGC 2 cut(s) 148, 576
AclI AACGTT 1 cut(s) 864
AclWI GGATC 2 cut(s) 242, 1043
AcoI YGGCCR 1 cut(s) 310
AcsI RAATTY 1 cut(s) 1043
AfaI GTAC 6 cut(s) 287, 696, 818, 1191, 1266, 1361
AfiI CCNNNNNNNGG 3 cut(s) 169, 212, 1442
AgsI TTSAA 7 cut(s) 136, 220, 338, 1042, 1084, 1100, 1278
AjnI CCWGG 3 cut(s) 247, 391, 1212
AjuI GAANNNNNNNTTGG 2 cut(s) 251, 283
AluBI AGCT 6 cut(s) 118, 341, 350, 430, 1106, 1428
AluI AGCT 6 cut(s) 118, 341, 350, 430, 1106, 1428
Alw26I GTCTC 2 cut(s) 525, 1396
AlwI GGATC 2 cut(s) 242, 1043
Ama87I CYCGRG 1 cut(s) 425
AoxI GGCC 3 cut(s) 310, 1088, 1381
ApeKI GCWGC 3 cut(s) 497, 593, 944
ApoI RAATTY 1 cut(s) 1043
Asp718I GGTACC 1 cut(s) 1264
AspS9I GGNCC 3 cut(s) 126, 389, 434
AsuHPI GGTGA 4 cut(s) 235, 354, 628, 1114
AvaI CYCGRG 1 cut(s) 425
AvaII GGWCC 3 cut(s) 126, 389, 434
AxyI CCTNAGG 1 cut(s) 207
BanI GGYRCC 1 cut(s) 1264
BanII GRGCYC 1 cut(s) 164
BbvI GCAGC 3 cut(s) 484, 605, 931
BccI CCATC 3 cut(s) 449, 1250, 1466
BceAI ACGGC 1 cut(s) 297
BcgI CGANNNNNNTGC 2 cut(s) 809, 843
BciT130I CCWGG 3 cut(s) 249, 393, 1214
BclI TGATCA 2 cut(s) 786, 907
BcoDI GTCTC 2 cut(s) 525, 1396
BfaI CTAG 1 cut(s) 1507
BfmI CTRYAG 1 cut(s) 1395
BisI GCNGC 3 cut(s) 498, 594, 945
BlpI GCTNAGC 1 cut(s) 342
BlsI GCNGC 3 cut(s) 499, 595, 946
Bme1390I CCNGG 3 cut(s) 249, 393, 1214
Bme18I GGWCC 3 cut(s) 126, 389, 434
BmeT110I CYCGRG 1 cut(s) 425
BmgT120I GGNCC 3 cut(s) 126, 389, 434
BmiI GGNNCC 3 cut(s) 1146, 1266, 1337
BmrFI CCNGG 3 cut(s) 249, 393, 1214
BmrI ACTGGG 1 cut(s) 268
BmsI GCATC 6 cut(s) 159, 559, 919, 931, 1190, 1423
BmuI ACTGGG 1 cut(s) 268
BplI GAGNNNNNCTC 2 cut(s) 408, 440
BpmI CTGGAG 1 cut(s) 1235
Bpu10I CCTNAGC 1 cut(s) 351
Bpu1102I GCTNAGC 1 cut(s) 342
BpuEI CTTGAG 2 cut(s) 536, 743
BsaJI CCNNGG 1 cut(s) 85
BsaXI ACNNNNNCTCC 2 cut(s) 1092, 1122
Bsc4I CCNNNNNNNGG 3 cut(s) 169, 212, 1442
Bse118I RCCGGY 1 cut(s) 308
Bse1I ACTGG 3 cut(s) 274, 1268, 1310
Bse21I CCTNAGG 1 cut(s) 207
Bse3DI GCAATG 3 cut(s) 324, 426, 931
BseBI CCWGG 3 cut(s) 249, 393, 1214
BseDI CCNNGG 1 cut(s) 85
BseGI GGATG 1 cut(s) 202
BseLI CCNNNNNNNGG 3 cut(s) 169, 212, 1442
BseMI GCAATG 3 cut(s) 324, 426, 931
BseMII CTCAG 3 cut(s) 221, 333, 1311
BseNI ACTGG 3 cut(s) 274, 1268, 1310
BseX3I CGGCCG 1 cut(s) 310
BseXI GCAGC 3 cut(s) 484, 605, 931
BseYI CCCAGC 2 cut(s) 527, 1355
BsgI GTGCAG 1 cut(s) 1075
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 3 cut(s) 312, 1090, 1383
BshNI GGYRCC 1 cut(s) 1264
BsiEI CGRYCG 1 cut(s) 313
BsiHKCI CYCGRG 1 cut(s) 425
BsiSI CCGG 1 cut(s) 309
BslI CCNNNNNNNGG 3 cut(s) 169, 212, 1442
BsmAI GTCTC 2 cut(s) 525, 1396
BsmI GAATGC 1 cut(s) 784
BsnI GGCC 3 cut(s) 312, 1090, 1383
BsoBI CYCGRG 1 cut(s) 425
Bsp1286I GDGCHC 1 cut(s) 164
Bsp143I GATC 4 cut(s) 234, 786, 907, 1048
Bsp1720I GCTNAGC 1 cut(s) 342
Bsp19I CCATGG 1 cut(s) 85
BspACI CCGC 2 cut(s) 148, 576
BspANI GGCC 3 cut(s) 312, 1090, 1383
BspCNI CTCAG 3 cut(s) 220, 334, 1312
BspLI GGNNCC 3 cut(s) 1146, 1266, 1337
BspPI GGATC 2 cut(s) 242, 1043
BspT107I GGYRCC 1 cut(s) 1264
BsrBI CCGCTC 1 cut(s) 576
BsrDI GCAATG 3 cut(s) 324, 426, 931
BsrFI RCCGGY 1 cut(s) 308
BsrI ACTGG 3 cut(s) 274, 1268, 1310
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 1 cut(s) 85
BssMI GATC 4 cut(s) 234, 786, 907, 1048
BssT1I CCWWGG 1 cut(s) 85
Bst2UI CCWGG 3 cut(s) 249, 393, 1214
Bst4CI ACNGT 5 cut(s) 295, 438, 881, 1189, 1399
Bst6I CTCTTC 3 cut(s) 57, 372, 1488
BstC8I GCNNGC 9 cut(s) 95, 105, 148, 310, 348, 574, 832, 1329, 1437
BstDEI CTNAG 5 cut(s) 207, 342, 351, 1320, 1340
BstDSI CCRYGG 1 cut(s) 85
BstENI CCTNNNNNAGG 2 cut(s) 210, 1440
BstF5I GGATG 1 cut(s) 202
BstKTI GATC 4 cut(s) 237, 789, 910, 1051
BstMAI GTCTC 2 cut(s) 525, 1396
BstMBI GATC 4 cut(s) 234, 786, 907, 1048
BstMCI CGRYCG 1 cut(s) 313
BstMWI GCNNNNNNNGC 5 cut(s) 314, 347, 416, 836, 1112
BstNI CCWGG 3 cut(s) 249, 393, 1214
BstSCI CCNGG 3 cut(s) 247, 391, 1212
BstSFI CTRYAG 1 cut(s) 1395
BstV1I GCAGC 3 cut(s) 484, 605, 931
BstXI CCANNNNNNTGG 2 cut(s) 281, 455
BstZI CGGCCG 1 cut(s) 310
Bsu36I CCTNAGG 1 cut(s) 207
BsuRI GGCC 3 cut(s) 312, 1090, 1383
BtgI CCRYGG 1 cut(s) 85
BtgZI GCGATG 1 cut(s) 468
BtsCI GGATG 1 cut(s) 202
BtsI GCAGTG 3 cut(s) 789, 1011, 1063
BtsIMutI CAGTG 6 cut(s) 789, 1011, 1063, 1249, 1275, 1404
Cac8I GCNNGC 9 cut(s) 95, 105, 148, 310, 348, 574, 832, 1329, 1437
Cfr10I RCCGGY 1 cut(s) 308
Cfr13I GGNCC 3 cut(s) 126, 389, 434
CsiI ACCWGGT 1 cut(s) 391
Csp6I GTAC 6 cut(s) 286, 695, 817, 1190, 1265, 1360
CviAII CATG 9 cut(s) 86, 486, 494, 836, 993, 1112, 1149, 1167, 1205
CviQI GTAC 6 cut(s) 286, 695, 817, 1190, 1265, 1360
DdeI CTNAG 5 cut(s) 207, 342, 351, 1320, 1340
DpnI GATC 4 cut(s) 236, 788, 909, 1050
DpnII GATC 4 cut(s) 234, 786, 907, 1048
EaeI YGGCCR 1 cut(s) 310
EagI CGGCCG 1 cut(s) 310
Eam1104I CTCTTC 3 cut(s) 57, 372, 1488
EarI CTCTTC 3 cut(s) 57, 372, 1488
EclXI CGGCCG 1 cut(s) 310
Eco130I CCWWGG 1 cut(s) 85
Eco147I AGGCCT 1 cut(s) 1383
Eco24I GRGCYC 1 cut(s) 164
Eco47I GGWCC 3 cut(s) 126, 389, 434
Eco52I CGGCCG 1 cut(s) 310
Eco81I CCTNAGG 1 cut(s) 207
Eco88I CYCGRG 1 cut(s) 425
EcoNI CCTNNNNNAGG 2 cut(s) 210, 1440
EcoO109I RGGNCCY 1 cut(s) 389
EcoRII CCWGG 3 cut(s) 247, 391, 1212
EcoT14I CCWWGG 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 85
FaeI CATG 9 cut(s) 89, 489, 497, 839, 996, 1115, 1152, 1170, 1208
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FatI CATG 9 cut(s) 85, 485, 493, 835, 992, 1111, 1148, 1166, 1204
FauI CCCGC 2 cut(s) 155, 569
FbaI TGATCA 2 cut(s) 786, 907
FblI GTMKAC 1 cut(s) 1001
Fnu4HI GCNGC 3 cut(s) 498, 594, 945
FokI GGATG 1 cut(s) 189
FriOI GRGCYC 1 cut(s) 164
Fsp4HI GCNGC 3 cut(s) 498, 594, 945
FspBI CTAG 1 cut(s) 1507
GluI GCNGC 3 cut(s) 498, 594, 945
GsaI CCCAGC 2 cut(s) 531, 1359
GsuI CTGGAG 1 cut(s) 1235
HaeIII GGCC 3 cut(s) 312, 1090, 1383
HapII CCGG 1 cut(s) 309
Hin1II CATG 9 cut(s) 89, 489, 497, 839, 996, 1115, 1152, 1170, 1208
HincII GTYRAC 1 cut(s) 967
HindII GTYRAC 1 cut(s) 967
HinfI GANTC 7 cut(s) 175, 356, 444, 490, 518, 1084, 1303
HpaII CCGG 1 cut(s) 309
HphI GGTGA 4 cut(s) 235, 354, 628, 1114
Hpy166II GTNNAC 3 cut(s) 967, 1002, 1362
Hpy188I TCNGA 3 cut(s) 443, 562, 850
Hpy188III TCNNGA 5 cut(s) 209, 638, 901, 1292, 1487
Hpy8I GTNNAC 3 cut(s) 967, 1002, 1362
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 5 cut(s) 175, 206, 214, 220, 999
HpyCH4III ACNGT 5 cut(s) 295, 438, 881, 1189, 1399
HpyCH4IV ACGT 6 cut(s) 141, 261, 288, 864, 963, 1095
HpyF10VI GCNNNNNNNGC 5 cut(s) 314, 347, 416, 836, 1112
HpyF3I CTNAG 5 cut(s) 207, 342, 351, 1320, 1340
HpySE526I ACGT 6 cut(s) 141, 261, 288, 864, 963, 1095
Hsp92II CATG 9 cut(s) 89, 489, 497, 839, 996, 1115, 1152, 1170, 1208
KpnI GGTACC 1 cut(s) 1268
KroI GCCGGC 1 cut(s) 308
KroNI GCCGGC 1 cut(s) 310
Ksp22I TGATCA 2 cut(s) 786, 907
Kzo9I GATC 4 cut(s) 234, 786, 907, 1048
LmnI GCTCC 2 cut(s) 123, 416
Lsp1109I GCAGC 3 cut(s) 484, 605, 931
LweI GCATC 6 cut(s) 159, 559, 919, 931, 1190, 1423
MabI ACCWGGT 1 cut(s) 391
MaeI CTAG 1 cut(s) 1507
MaeII ACGT 6 cut(s) 141, 261, 288, 864, 963, 1095
MaeIII GTNAC 3 cut(s) 289, 1120, 1270
MalI GATC 4 cut(s) 236, 788, 909, 1050
MbiI CCGCTC 1 cut(s) 576
MboI GATC 4 cut(s) 234, 786, 907, 1048
MboII GAAGA 9 cut(s) 44, 148, 170, 351, 359, 362, 393, 1298, 1475
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 9 cut(s) 24, 42, 131, 242, 601, 619, 771, 883, 1043
MlyI GAGTC 1 cut(s) 527
MmeI TCCRAC 1 cut(s) 606
MroNI GCCGGC 1 cut(s) 308
MseI TTAA 2 cut(s) 240, 870
MslI CAYNNNNRTG 2 cut(s) 453, 1116
MspI CCGG 1 cut(s) 309
MspR9I CCNGG 3 cut(s) 249, 393, 1214
Mva1269I GAATGC 1 cut(s) 784
MvaI CCWGG 3 cut(s) 249, 393, 1214
MwoI GCNNNNNNNGC 5 cut(s) 314, 347, 416, 836, 1112
NaeI GCCGGC 1 cut(s) 310
NcoI CCATGG 1 cut(s) 85
NdeII GATC 4 cut(s) 234, 786, 907, 1048
NgoMIV GCCGGC 1 cut(s) 308
NlaIII CATG 9 cut(s) 89, 489, 497, 839, 996, 1115, 1152, 1170, 1208
NlaIV GGNNCC 3 cut(s) 1146, 1266, 1337
NmuCI GTSAC 2 cut(s) 1120, 1270
PaeR7I CTCGAG 1 cut(s) 425
PceI AGGCCT 1 cut(s) 1383
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PctI GAATGC 1 cut(s) 784
PdiI GCCGGC 1 cut(s) 310
PfeI GAWTC 6 cut(s) 175, 356, 444, 490, 1084, 1303
PfoI TCCNGGA 1 cut(s) 1212
PkrI GCNGC 3 cut(s) 499, 595, 946
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
PpuMI RGGWCCY 1 cut(s) 389
PsiI TTATAA 1 cut(s) 975
Psp1406I AACGTT 1 cut(s) 864
Psp5II RGGWCCY 1 cut(s) 389
Psp6I CCWGG 3 cut(s) 247, 391, 1212
PspFI CCCAGC 2 cut(s) 527, 1355
PspGI CCWGG 3 cut(s) 247, 391, 1212
PspN4I GGNNCC 3 cut(s) 1146, 1266, 1337
PspPI GGNCC 3 cut(s) 126, 389, 434
PspPPI RGGWCCY 1 cut(s) 389
PspXI VCTCGAGB 1 cut(s) 425
RsaI GTAC 6 cut(s) 287, 696, 818, 1191, 1266, 1361
RsaNI GTAC 6 cut(s) 286, 695, 817, 1190, 1265, 1360
RseI CAYNNNNRTG 2 cut(s) 453, 1116
SaqAI TTAA 2 cut(s) 240, 870
SatI GCNGC 3 cut(s) 498, 594, 945
Sau3AI GATC 4 cut(s) 234, 786, 907, 1048
Sau96I GGNCC 3 cut(s) 126, 389, 434
SchI GAGTC 1 cut(s) 527
ScrFI CCNGG 3 cut(s) 249, 393, 1214
SduI GDGCHC 1 cut(s) 164
SexAI ACCWGGT 1 cut(s) 391
SfaNI GCATC 6 cut(s) 159, 559, 919, 931, 1190, 1423
SfcI CTRYAG 1 cut(s) 1395
Sfr274I CTCGAG 1 cut(s) 425
SinI GGWCC 3 cut(s) 126, 389, 434
SlaI CTCGAG 1 cut(s) 425
SmiMI CAYNNNNRTG 2 cut(s) 453, 1116
SmlI CTYRAG 3 cut(s) 425, 515, 758
SmoI CTYRAG 3 cut(s) 425, 515, 758
Sse9I AATT 9 cut(s) 24, 42, 131, 242, 601, 619, 771, 883, 1043
SseBI AGGCCT 1 cut(s) 1383
SsiI CCGC 2 cut(s) 148, 576
SspMI CTAG 1 cut(s) 1507
StuI AGGCCT 1 cut(s) 1383
StyD4I CCNGG 3 cut(s) 247, 391, 1212
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 5 cut(s) 295, 438, 881, 1189, 1399
TaiI ACGT 6 cut(s) 144, 264, 291, 867, 966, 1098
TaqI TCGA 5 cut(s) 153, 426, 1034, 1228, 1374
TasI AATT 9 cut(s) 24, 42, 131, 242, 601, 619, 771, 883, 1043
TfiI GAWTC 6 cut(s) 175, 356, 444, 490, 1084, 1303
Tru1I TTAA 2 cut(s) 240, 870
Tru9I TTAA 2 cut(s) 240, 870
TscAI CASTG 6 cut(s) 789, 1018, 1063, 1249, 1275, 1404
TseFI GTSAC 2 cut(s) 1120, 1270
TseI GCWGC 3 cut(s) 497, 593, 944
Tsp45I GTSAC 2 cut(s) 1120, 1270
TspDTI ATGAA 6 cut(s) 17, 474, 531, 810, 981, 1316
TspGWI ACGGA 1 cut(s) 1041
TspRI CASTG 6 cut(s) 789, 1018, 1063, 1249, 1275, 1404
VpaK11BI GGWCC 3 cut(s) 126, 389, 434
XagI CCTNNNNNAGG 2 cut(s) 210, 1440
XapI RAATTY 1 cut(s) 1043
XhoI CTCGAG 1 cut(s) 425
XmiI GTMKAC 1 cut(s) 1001
XspI CTAG 1 cut(s) 1507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.