FvH4_7g01980

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0015798
Physical Location & Seq
Forward (+)
2374709 .. 2381141
6433 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01980.t1

Sequence Viewer

Length: 3117 bp
ATGAGGTCTCGTATGGAGGACTACTTGAGAAAAAACTTCGACGTCGAACCAAAACGACCTTCCAACGAGGCCCTCAGGCGCTGGAGAGATGCCGTCTCCGTCGTCAAGAACCCTCGCCGCCGCTTCCGCATGGTCGCCGATCTCGCCAAGCGCGCCGAAGACGATAAAAAGCGCAAGAAACTCCAGGAAAAGATACGAGTAGCTCTTTATGTGCAAAAAGCAGCATTGCAATTCATCGATGCTGGTAAAGAAGCTCGTGGATACAGCCTTTCCAAACAAGTTAGTGATGCTGGTTTTAGCATTTCACCTGACGAGCTTGCATCATTTGTGCGATCCCATGATAATAAGGGTTTGGAAGCACGAGGGGGAGTTGAAGGAGTAGCAGGAAATATATCAGTATCCTTGAAAGAAGGTGTCGTGTCAAGCAAGATACCCGGTAGACAAGATGTATTTGGCCTCAACCGATTTATGGAGAAGCCTTCTAAAGGCTTTTGGATGTTCGTCTGGGAAGCCATACAAGATTTAACTCTAATCATCCTTATGGTTTCTGCTGTGGTCTCTATCGGCGTTGGCATTGCAACTGAAGGGTGGCCAAAGGGTATGTATGATGGGTTGGGTATAATACTCAGCATCTTGTTGGTTGTGATGGTCACTGCAATCAGTGACTACAGGCAGTCTTTGCAATTCAAAGAACTGGACAAGGAGAAAAAAAATATAATGATTCAGGTAACTAGGGATGGACGCAGACAGAAGGTTTCTATCTACGATTTGGTAGTTGGAGAGGTTGTTCATCTTTCAATTGGAGATCAAGTTCCAGCTGATGGCCTTCTCATATCAGGCCACAGCTTACAAATCGATGAATCAAGCTTGTCAGGTGAGAGTGAGCCGGTGGACATAAATCAAGATAGACCTTTTATTCTTGGAGGAACCAAAGTGCAAGATGGCTCTGGTAAAATGCTAGTTACATCAGTTGGTATGAGGACTGAGTGGGGACAGCTAATGGTTACTCTGAGTGAGGGAGGAGACAATGAGACACCACTACAGGTGAAACTCAATGGCGTTGCAACCATTATTGGAAAAATTGGTTTGGCATTTGCAGCTCTTACATTTGTTGTTCTGACTACGAGGTACTTAGTTAACAAGGGAATACACCATGACATGACGAACTGGTCTATGAATGATGCATCAGCACTTTTGAATTATTTCTCTACTGCAGTTATCATAATAGTTGTTGCTGTTCCTGAGGGGCTTCCTCTAGCAGTGACACTGAGTCTTGCCTTTGCAATGAAAAAACTAATGGGTGATAAGGCACTTGTGAGGCATCTTTCAGCATGCGAGACCATGGGATCCGCTAATTGTATTTGCACAGATAAGACGGGAACCTTGACAACAAATCATATGGTGGTGAATAAGATATGGATCTGTGACGAAGCCAAGAGTGCTCAGGACAAAGATGTATTGAAGTCAGTGTCTGAAAAAGTTTATAACCTCCTCTTGCAGTCTATCTTTCTGAATACAACCTCAGAGGTAGTAAAAGGGGAAGATGGGAAAAATAGTATTATAGGTACTCCAACAGAGATGGCAATAGTAGAATTTGGCATGCTTCTGGGAGGTGATTTTAAATCTTTCAGTGAGAAGTATAAGATAGATATGGTGGAACCATTCAATTCAATAAAAAAAAAGATGTCAGTGCAAGTGGCTCTTCCTGGTAGTGATATGTCTCGAGCATTCTGCAAAGGCGCATCAGAAATAGTTTTAGGAATGTGTGACAAGGTTGTCAATACTGATGGTGAAGCTTTGCCTTTGTCTGAAGAGCAAAGAAACAAAATATCTGATGTCATAAATGGTTTTGCTTGTGAAGCTTTAAGAACTCTATGCGTAGCTTTCAAGGATATAGAGAGCCCCTCTGGTGCGGAAAGTATTCCTGAAGATGGATATATATTAATAGCTGTTGTGGGAATTAAGGATCCTGTGCGCCCTGCCGTGAGGGAAGCAGTGAAGACTTGTTTAGATGCTGGGATCACTGTGCGGATGGTCACTGGTGATAATATCAATACAGCTAAAGCCATAGCTAAAGAGTGTGGCATTTTGACAGAAGATGGTTTAGCTATAGAAGGGCCAGATTTCCGAAAAATGAACGAACAAGAGATGGCAGAAATTATCCCAAAACTGCAGGTGATGGCACGATCCTTGCCTTTGGACAAGCACACTTTAGTGAAGCAGTTGAGGAATGTGCATAAAGATGTAGTGGCAGTAACTGGTGATGGGACTAATGATGCTCCTGCTTTGCATGAGGCAGACATTGGCCTCGCTATGGGTATAGCTGGTACAGAGGTTGCGAAAGAAAATGCTGATGTTATCATAATGGATGATAACTTTACAAGTATAGTGAATGTTGCAAGATGGGGCCGTGCAGTTTATATCAACATCCAAAAGTTTGTTCAGTTCCAGTTAACGGTTAACATTGTCGCTCTGATGCTCAATTTTGTATCAGCATGCGTCTCAGGATCGGCTCCTCTCACTGCTGTTCAAATGCTTTGGGTAAACTTGATCATGGATACTCTTGGTGCTTTGGCACTGGCTACAGAGCCACCACATGATGGCCTTATGAGAAGACCTCCAGTTGGAAGGAATACTAATTTCATCACTGGGATCATGTGGAGGAATATCATTGGTCAGAGTATCTATCAAATTACTGTCCTTCTGGTTCTTAATTTCTGTGGTATGCGTCTTCTAACTATCACCGGTCCGAAAGCAAGTTCTACTCTCAAGACCTTCATATTCAACAGTTTTGTATTTTGCCAGGTATTCAATGAGATAAACAGCCGTGATATGGAGAAAATCAATGTGTTCGGAGGGATGTTTCAAAGTTATACTTTCATGGTAGTCATGATCTGCACAGTAGTGTTCCAAATCCTAATAATTGAATTTTTGGGCACTTTTGCTCAAACAGTTCCACTGAGCTGGGAGTTGTGGTTAGCAAGCGTCTTGATTGGAGCCGTAAGTTTACTGGTCGCCGTTGTCTTGAAGTGCATTCCTGTCTTAATAAGGAAACAAGATGCTAAGGATCACGACGATATCCACGAACCTCTCCTCCGTGGCCCTGAGCTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1039

Amino Acids

113.76

Weight (kDa)

6.31

Isoelectric Point (pI)

38.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaATP_NAI PF12515 9 - 53 2.4e-20 Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N PF00690 121 - 187 4e-11 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 241 - 334 1e-20 P-type ATPase actuator domain
Cation_ATPase PF13246 495 - 660 5.7e-31 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 647 - 767 1.2e-10 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 838 - 1011 3.9e-41 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015798)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01980 FvH4_7g01980
malus_domestica MD02G1298100.v1.1 MD07G1026000.v1.1
prunus_persica Prupe.2G026200_v2.0.a1
pyrus_communis pycom07g01980
rosa_chinensis RchiOBHm_Chr1g0318981
rosa_laevigata RLG00000030512
rosa_multiflora Rmu_sc0003503.1_g000001 Rmu_sc0006626.1_g000004
rosa_roxburghii Rroxscaffold_4G00328710
rosa_rugosa Rorug01G0026100 Rorug01G0026200
rosa_samantha Rh1AG038000
rosa_wichuraiana Rw1G002930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1485
AarI CACCTGC 1 cut(s) 2164
AasI GACNNNNNNGTC 2 cut(s) 1168, 1775
AatII GACGTC 1 cut(s) 45
Acc36I ACCTGC 1 cut(s) 2164
AccB7I CCANNNNNTGG 2 cut(s) 821, 2600
AccI GTMKAC 1 cut(s) 439
AccII CGCG 1 cut(s) 153
AciI CCGC 6 cut(s) 118, 121, 127, 1350, 1913, 2029
AcoI YGGCCR 1 cut(s) 590
AcsI RAATTY 2 cut(s) 1592, 2927
AcuI CTGAAG 3 cut(s) 603, 1830, 1947
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 3 cut(s) 1130, 1567, 2329
AgeI ACCGGT 1 cut(s) 2744
AhdI GACNNNNNGTC 1 cut(s) 1269
AjnI CCWGG 3 cut(s) 183, 1705, 2802
AjuI GAANNNNNNNTTGG 4 cut(s) 43, 75, 2424, 2456
AleI CACNNNNGTG 2 cut(s) 2213, 2903
AloI GAACNNNNNNTCC 6 cut(s) 771, 795, 803, 827, 3078, 3110
Alw21I GWGCWC 1 cut(s) 1444
Alw26I GTCTC 8 cut(s) 12, 100, 562, 1017, 1025, 1331, 1725, 2506
AlwNI CAGNNNCTG 3 cut(s) 81, 1472, 2258
Ama87I CYCGRG 1 cut(s) 1722
ApeKI GCWGC 2 cut(s) 221, 1097
ApoI RAATTY 2 cut(s) 1592, 2927
AseI ATTAAT 1 cut(s) 1943
AsiGI ACCGGT 1 cut(s) 2744
Asp700I GAANNNNTTC 2 cut(s) 1202, 1920
AspLEI GCGC 6 cut(s) 81, 153, 155, 174, 1742, 1977
AspS9I GGNCC 5 cut(s) 70, 2117, 2407, 2747, 3101
AsuC2I CCSGG 1 cut(s) 435
AvaI CYCGRG 1 cut(s) 1722
AvaII GGWCC 1 cut(s) 2747
AxyI CCTNAGG 2 cut(s) 74, 1242
BaeGI GKGCMC 1 cut(s) 2939
BaeI ACNNNNGTAYC 2 cut(s) 2319, 2352
BalI TGGCCA 1 cut(s) 592
BamHI GGATCC 2 cut(s) 1346, 1966
BanII GRGCYC 1 cut(s) 1904
BauI CACGAG 2 cut(s) 255, 360
BbsI GAAGAC 4 cut(s) 165, 2006, 2620, 2723
Bbv12I GWGCWC 1 cut(s) 1444
BbvI GCAGC 2 cut(s) 233, 1109
BceAI ACGGC 6 cut(s) 77, 1967, 2394, 2811, 2984, 3002
BcgI CGANNNNNNTGC 2 cut(s) 1713, 1747
BciT130I CCWGG 3 cut(s) 185, 1707, 2804
BciVI GTATCC 3 cut(s) 254, 409, 2551
BclI TGATCA 1 cut(s) 2550
BcnI CCSGG 1 cut(s) 435
BcoDI GTCTC 8 cut(s) 12, 100, 562, 1017, 1025, 1331, 1725, 2506
BfaI CTAG 3 cut(s) 732, 959, 1256
BfmI CTRYAG 6 cut(s) 667, 1040, 1212, 2109, 2171, 2583
BfoI RGCGCY 1 cut(s) 82
BfuAI ACCTGC 1 cut(s) 2164
BfuI GTATCC 3 cut(s) 254, 409, 2551
BisI GCNGC 4 cut(s) 118, 121, 222, 1098
BlsI GCNGC 4 cut(s) 119, 122, 223, 1099
Bme1390I CCNGG 4 cut(s) 185, 435, 1707, 2804
Bme18I GGWCC 1 cut(s) 2747
BmeRI GACNNNNNGTC 1 cut(s) 1269
BmeT110I CYCGRG 1 cut(s) 1722
BmgT120I GGNCC 5 cut(s) 70, 2117, 2407, 2747, 3101
BmiI GGNNCC 8 cut(s) 928, 1348, 1381, 1659, 1968, 2408, 2514, 2998
BmrFI CCNGG 4 cut(s) 185, 435, 1707, 2804
BmrI ACTGGG 1 cut(s) 2658
BmuI ACTGGG 1 cut(s) 2658
BpiI GAAGAC 4 cut(s) 165, 2006, 2620, 2723
BplI GAGNNNNNCTC 4 cut(s) 1889, 1921, 2602, 2634
BpmI CTGGAG 3 cut(s) 103, 167, 2604
Bpu10I CCTNAGC 3 cut(s) 1443, 3063, 3105
BpuEI CTTGAG 2 cut(s) 46, 2753
BpuMI CCSGG 1 cut(s) 435
Bsa29I ATCGAT 2 cut(s) 237, 855
BsaBI GATNNNNATC 1 cut(s) 1418
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 3 cut(s) 12, 562, 1331
BsaJI CCNNGG 2 cut(s) 1341, 3097
BsaWI WCCGGW 1 cut(s) 2744
BsaXI ACNNNNNCTCC 8 cut(s) 771, 795, 801, 825, 2960, 2990, 3078, 3108
Bse118I RCCGGY 2 cut(s) 886, 2744
Bse1I ACTGG 9 cut(s) 699, 1172, 2044, 2263, 2449, 2583, 2621, 2653, 3015
Bse21I CCTNAGG 2 cut(s) 74, 1242
Bse3DI GCAATG 3 cut(s) 224, 573, 1290
Bse8I GATNNNNATC 1 cut(s) 1418
BseBI CCWGG 3 cut(s) 185, 1707, 2804
BseCI ATCGAT 2 cut(s) 237, 855
BseDI CCNNGG 2 cut(s) 1341, 3097
BseGI GGATG 7 cut(s) 501, 534, 742, 2037, 2374, 2427, 2865
BseJI GATNNNNATC 1 cut(s) 1418
BseMI GCAATG 3 cut(s) 224, 573, 1290
BseNI ACTGG 9 cut(s) 699, 1172, 2044, 2263, 2449, 2583, 2621, 2653, 3015
BsePI GCGCGC 1 cut(s) 151
BseRI GAGGAG 4 cut(s) 1035, 1481, 2505, 3083
BseSI GKGCMC 1 cut(s) 2939
BseXI GCAGC 2 cut(s) 233, 1109
BseYI CCCAGC 2 cut(s) 2015, 2964
BsgI GTGCAG 2 cut(s) 2433, 2881
Bsh1236I CGCG 1 cut(s) 153
BshTI ACCGGT 1 cut(s) 2744
BshVI ATCGAT 2 cut(s) 237, 855
BsiHKAI GWGCWC 1 cut(s) 1444
BsiHKCI CYCGRG 1 cut(s) 1722
BsiSI CCGG 3 cut(s) 435, 887, 2745
BslFI GGGAC 2 cut(s) 1005, 2281
BsmAI GTCTC 8 cut(s) 12, 100, 562, 1017, 1025, 1331, 1725, 2506
BsmBI CGTCTC 2 cut(s) 100, 2506
BsmFI GGGAC 2 cut(s) 1005, 2281
BsmI GAATGC 2 cut(s) 1727, 3033
Bso31I GGTCTC 3 cut(s) 12, 562, 1331
BsoBI CYCGRG 1 cut(s) 1722
Bsp1286I GDGCHC 3 cut(s) 1444, 1904, 2939
Bsp19I CCATGG 1 cut(s) 1341
BspACI CCGC 6 cut(s) 118, 121, 127, 1350, 1913, 2029
BspDI ATCGAT 2 cut(s) 237, 855
BspFNI CGCG 1 cut(s) 153
BspHI TCATGA 1 cut(s) 2889
BspLI GGNNCC 8 cut(s) 928, 1348, 1381, 1659, 1968, 2408, 2514, 2998
BspMAI CTGCAG 2 cut(s) 1216, 2175
BspMI ACCTGC 1 cut(s) 2164
BspQI GCTCTTC 2 cut(s) 1707, 1806
BspTNI GGTCTC 3 cut(s) 12, 562, 1331
BsrDI GCAATG 3 cut(s) 224, 573, 1290
BsrFI RCCGGY 2 cut(s) 886, 2744
BsrI ACTGG 9 cut(s) 699, 1172, 2044, 2263, 2449, 2583, 2621, 2653, 3015
BssAI RCCGGY 2 cut(s) 886, 2744
BssECI CCNNGG 2 cut(s) 1341, 3097
BssHII GCGCGC 1 cut(s) 151
BssNI GRCGYC 1 cut(s) 42
BssSI CACGAG 2 cut(s) 255, 360
BssT1I CCWWGG 1 cut(s) 1341
Bst2BI CACGAG 2 cut(s) 255, 360
Bst2UI CCWGG 3 cut(s) 185, 1707, 2804
Bst4CI ACNGT 6 cut(s) 2026, 2458, 2698, 2789, 2902, 2953
Bst6I CTCTTC 2 cut(s) 1707, 1806
BstACI GRCGYC 1 cut(s) 42
BstAPI GCANNNNNTGC 1 cut(s) 679
BstC8I GCNNGC 7 cut(s) 153, 318, 1333, 1601, 2497, 2983, 3111
BstDSI CCRYGG 2 cut(s) 1341, 3097
BstENI CCTNNNNNAGG 1 cut(s) 483
BstF5I GGATG 7 cut(s) 501, 534, 742, 2037, 2374, 2427, 2865
BstFNI CGCG 1 cut(s) 153
BstH2I RGCGCY 1 cut(s) 82
BstHHI GCGC 6 cut(s) 81, 153, 155, 174, 1742, 1977
BstMAI GTCTC 8 cut(s) 12, 100, 562, 1017, 1025, 1331, 1725, 2506
BstMWI GCNNNNNNNGC 7 cut(s) 126, 143, 152, 679, 1097, 1439, 1859
BstNI CCWGG 3 cut(s) 185, 1707, 2804
BstNSI RCATGY 3 cut(s) 1335, 1603, 2499
BstSCI CCNGG 4 cut(s) 183, 433, 1705, 2802
BstSFI CTRYAG 6 cut(s) 667, 1040, 1212, 2109, 2171, 2583
BstSLI GKGCMC 1 cut(s) 2939
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 2 cut(s) 233, 1109
BstV2I GAAGAC 4 cut(s) 165, 2006, 2620, 2723
BstX2I RGATCY 3 cut(s) 1346, 1419, 1966
BstXI CCANNNNNNTGG 1 cut(s) 2964
BstYI RGATCY 3 cut(s) 1346, 1419, 1966
Bsu15I ATCGAT 2 cut(s) 237, 855
Bsu36I CCTNAGG 2 cut(s) 74, 1242
BsuI GTATCC 3 cut(s) 254, 409, 2551
BsuTUI ATCGAT 2 cut(s) 237, 855
BtgI CCRYGG 2 cut(s) 1341, 3097
BtsCI GGATG 7 cut(s) 501, 534, 742, 2037, 2374, 2427, 2865
BtsI GCAGTG 4 cut(s) 651, 1266, 2001, 2520
BveI ACCTGC 1 cut(s) 2164
Cac8I GCNNGC 7 cut(s) 153, 318, 1333, 1601, 2497, 2983, 3111
CaiI CAGNNNCTG 3 cut(s) 81, 1472, 2258
CciI TCATGA 1 cut(s) 2889
CfoI GCGC 6 cut(s) 81, 153, 155, 174, 1742, 1977
Cfr10I RCCGGY 2 cut(s) 886, 2744
Cfr13I GGNCC 5 cut(s) 70, 2117, 2407, 2747, 3101
ClaI ATCGAT 2 cut(s) 237, 855
CpoI CGGWCCG 1 cut(s) 2747
CseI GACGC 4 cut(s) 750, 2488, 2717, 2974
Csp6I GTAC 3 cut(s) 1129, 1566, 2328
CspAI ACCGGT 1 cut(s) 2744
CspI CGGWCCG 1 cut(s) 2747
CviQI GTAC 3 cut(s) 1129, 1566, 2328
DraI TTTAAA 1 cut(s) 1621
DrdI GACNNNNNNGTC 2 cut(s) 1168, 1775
DriI GACNNNNNGTC 1 cut(s) 1269
DseDI GACNNNNNNGTC 2 cut(s) 1168, 1775
EaeI YGGCCR 1 cut(s) 590
Eam1104I CTCTTC 2 cut(s) 1707, 1806
Eam1105I GACNNNNNGTC 1 cut(s) 1269
EarI CTCTTC 2 cut(s) 1707, 1806
Eco130I CCWWGG 1 cut(s) 1341
Eco24I GRGCYC 1 cut(s) 1904
Eco31I GGTCTC 3 cut(s) 12, 562, 1331
Eco32I GATATC 1 cut(s) 3079
Eco47I GGWCC 1 cut(s) 2747
Eco57I CTGAAG 3 cut(s) 603, 1830, 1947
Eco81I CCTNAGG 2 cut(s) 74, 1242
Eco88I CYCGRG 1 cut(s) 1722
EcoNI CCTNNNNNAGG 1 cut(s) 483
EcoO109I RGGNCCY 1 cut(s) 70
EcoRII CCWGG 3 cut(s) 183, 1705, 2802
EcoRV GATATC 1 cut(s) 3079
EcoT14I CCWWGG 1 cut(s) 1341
EcoT22I ATGCAT 1 cut(s) 1186
EcoT38I GRGCYC 1 cut(s) 1904
ErhI CCWWGG 1 cut(s) 1341
Esp3I CGTCTC 2 cut(s) 100, 2506
FalI AAGNNNNNCTT 4 cut(s) 1686, 1718, 2860, 2892
FaqI GGGAC 2 cut(s) 1005, 2281
FauNDI CATATG 1 cut(s) 1398
FbaI TGATCA 1 cut(s) 2550
FblI GTMKAC 1 cut(s) 439
Fnu4HI GCNGC 4 cut(s) 118, 121, 222, 1098
FokI GGATG 7 cut(s) 508, 521, 749, 2044, 2381, 2414, 2872
FriOI GRGCYC 1 cut(s) 1904
Fsp4HI GCNGC 4 cut(s) 118, 121, 222, 1098
FspBI CTAG 3 cut(s) 732, 959, 1256
GlaI GCGC 6 cut(s) 80, 152, 154, 173, 1741, 1976
GluI GCNGC 4 cut(s) 118, 121, 222, 1098
GsaI CCCAGC 2 cut(s) 2019, 2968
GsuI CTGGAG 3 cut(s) 103, 167, 2604
HaeII RGCGCY 1 cut(s) 82
HapII CCGG 3 cut(s) 435, 887, 2745
HgaI GACGC 4 cut(s) 750, 2488, 2717, 2974
HhaI GCGC 6 cut(s) 81, 153, 155, 174, 1742, 1977
Hin1I GRCGYC 1 cut(s) 42
Hin6I GCGC 6 cut(s) 79, 151, 153, 172, 1740, 1975
HinP1I GCGC 6 cut(s) 79, 151, 153, 172, 1740, 1975
HincII GTYRAC 3 cut(s) 1138, 2454, 2461
HindII GTYRAC 3 cut(s) 1138, 2454, 2461
HindIII AAGCTT 3 cut(s) 865, 1794, 1860
HinfI GANTC 3 cut(s) 721, 860, 1270
HpaI GTTAAC 3 cut(s) 1138, 2454, 2461
HpaII CCGG 3 cut(s) 435, 887, 2745
Hpy166II GTNNAC 7 cut(s) 440, 892, 1138, 2454, 2461, 2545, 3008
Hpy8I GTNNAC 7 cut(s) 440, 892, 1138, 2454, 2461, 2545, 3008
Hpy99I CGWCG 4 cut(s) 44, 47, 104, 3077
HpyCH4III ACNGT 6 cut(s) 2026, 2458, 2698, 2789, 2902, 2953
HpyCH4IV ACGT 1 cut(s) 42
HpyF10VI GCNNNNNNNGC 7 cut(s) 126, 143, 152, 679, 1097, 1439, 1859
HpySE526I ACGT 1 cut(s) 42
Hsp92I GRCGYC 1 cut(s) 42
HspAI GCGC 6 cut(s) 79, 151, 153, 172, 1740, 1975
Ksp22I TGATCA 1 cut(s) 2550
KspAI GTTAAC 3 cut(s) 1138, 2454, 2461
LguI GCTCTTC 2 cut(s) 1707, 1806
LmnI GCTCC 3 cut(s) 2284, 2518, 2996
Lsp1109I GCAGC 2 cut(s) 233, 1109
MaeI CTAG 3 cut(s) 732, 959, 1256
MaeII ACGT 1 cut(s) 42
MboII GAAGA 9 cut(s) 170, 1553, 1694, 1823, 1940, 2011, 2108, 2625, 2723
MfeI CAATTG 1 cut(s) 798
MflI RGATCY 3 cut(s) 1346, 1419, 1966
MhlI GDGCHC 3 cut(s) 1444, 1904, 2939
MlsI TGGCCA 1 cut(s) 592
MluNI TGGCCA 1 cut(s) 592
MlyI GAGTC 1 cut(s) 1279
MmeI TCCRAC 4 cut(s) 87, 757, 1595, 2605
Mox20I TGGCCA 1 cut(s) 592
Mph1103I ATGCAT 1 cut(s) 1186
MroXI GAANNNNTTC 2 cut(s) 1202, 1920
MscI TGGCCA 1 cut(s) 592
MslI CAYNNNNRTG 4 cut(s) 539, 2213, 2241, 2903
Msp20I TGGCCA 1 cut(s) 592
MspA1I CMGCKG 1 cut(s) 818
MspI CCGG 3 cut(s) 435, 887, 2745
MspR9I CCNGG 4 cut(s) 185, 435, 1707, 2804
MunI CAATTG 1 cut(s) 798
Mva1269I GAATGC 2 cut(s) 1727, 3033
MvaI CCWGG 3 cut(s) 185, 1707, 2804
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 7 cut(s) 126, 143, 152, 679, 1097, 1439, 1859
NciI CCSGG 1 cut(s) 435
NcoI CCATGG 1 cut(s) 1341
NdeI CATATG 1 cut(s) 1398
NlaIV GGNNCC 8 cut(s) 928, 1348, 1381, 1659, 1968, 2408, 2514, 2998
NmuCI GTSAC 6 cut(s) 649, 662, 1261, 1424, 1766, 2035
NsiI ATGCAT 1 cut(s) 1186
NspI RCATGY 3 cut(s) 1335, 1603, 2499
OliI CACNNNNGTG 2 cut(s) 2213, 2903
PaeI GCATGC 3 cut(s) 1335, 1603, 2499
PaeR7I CTCGAG 1 cut(s) 1722
PagI TCATGA 1 cut(s) 2889
PaqCI CACCTGC 1 cut(s) 2164
PauI GCGCGC 1 cut(s) 151
PciSI GCTCTTC 2 cut(s) 1707, 1806
PcsI WCGNNNNNNNCGW 1 cut(s) 3081
PctI GAATGC 2 cut(s) 1727, 3033
PdmI GAANNNNTTC 2 cut(s) 1202, 1920
PfeI GAWTC 2 cut(s) 721, 860
PflMI CCANNNNNTGG 2 cut(s) 821, 2600
PfoI TCCNGGA 1 cut(s) 183
PinAI ACCGGT 1 cut(s) 2744
PkrI GCNGC 4 cut(s) 119, 122, 223, 1099
PleI GAGTC 1 cut(s) 1278
PpsI GAGTC 1 cut(s) 1278
PshBI ATTAAT 1 cut(s) 1943
PsiI TTATAA 1 cut(s) 1485
Psp6I CCWGG 3 cut(s) 183, 1705, 2802
PspFI CCCAGC 2 cut(s) 2015, 2964
PspGI CCWGG 3 cut(s) 183, 1705, 2802
PspN4I GGNNCC 8 cut(s) 928, 1348, 1381, 1659, 1968, 2408, 2514, 2998
PspPI GGNCC 5 cut(s) 70, 2117, 2407, 2747, 3101
PstI CTGCAG 2 cut(s) 1216, 2175
PstNI CAGNNNCTG 3 cut(s) 81, 1472, 2258
PsuI RGATCY 3 cut(s) 1346, 1419, 1966
PteI GCGCGC 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 818
RsaI GTAC 3 cut(s) 1130, 1567, 2329
RsaNI GTAC 3 cut(s) 1129, 1566, 2328
RseI CAYNNNNRTG 4 cut(s) 539, 2213, 2241, 2903
Rsr2I CGGWCCG 1 cut(s) 2747
RsrII CGGWCCG 1 cut(s) 2747
SapI GCTCTTC 2 cut(s) 1707, 1806
SatI GCNGC 4 cut(s) 118, 121, 222, 1098
Sau96I GGNCC 5 cut(s) 70, 2117, 2407, 2747, 3101
SchI GAGTC 1 cut(s) 1279
ScrFI CCNGG 4 cut(s) 185, 435, 1707, 2804
SduI GDGCHC 3 cut(s) 1444, 1904, 2939
SfcI CTRYAG 6 cut(s) 667, 1040, 1212, 2109, 2171, 2583
Sfr274I CTCGAG 1 cut(s) 1722
SinI GGWCC 1 cut(s) 2747
SlaI CTCGAG 1 cut(s) 1722
SmiMI CAYNNNNRTG 4 cut(s) 539, 2213, 2241, 2903
SmlI CTYRAG 3 cut(s) 25, 1722, 2768
SmoI CTYRAG 3 cut(s) 25, 1722, 2768
SphI GCATGC 3 cut(s) 1335, 1603, 2499
SsiI CCGC 6 cut(s) 118, 121, 127, 1350, 1913, 2029
SspMI CTAG 3 cut(s) 732, 959, 1256
StyD4I CCNGG 4 cut(s) 183, 433, 1705, 2802
StyI CCWWGG 1 cut(s) 1341
TaaI ACNGT 6 cut(s) 2026, 2458, 2698, 2789, 2902, 2953
TaiI ACGT 1 cut(s) 45
TaqI TCGA 5 cut(s) 39, 45, 237, 855, 1723
TauI GCSGC 2 cut(s) 120, 123
TfiI GAWTC 2 cut(s) 721, 860
TseFI GTSAC 6 cut(s) 649, 662, 1261, 1424, 1766, 2035
TseI GCWGC 2 cut(s) 221, 1097
Tsp45I GTSAC 6 cut(s) 649, 662, 1261, 1424, 1766, 2035
TspDTI ATGAA 9 cut(s) 223, 779, 873, 1190, 1301, 2150, 2632, 2767, 2869
TspGWI ACGGA 2 cut(s) 88, 3086
Van91I CCANNNNNTGG 2 cut(s) 821, 2600
VpaK11BI GGWCC 1 cut(s) 2747
VspI ATTAAT 1 cut(s) 1943
XagI CCTNNNNNAGG 1 cut(s) 483
XapI RAATTY 2 cut(s) 1592, 2927
XceI RCATGY 3 cut(s) 1335, 1603, 2499
XhoI CTCGAG 1 cut(s) 1722
XmiI GTMKAC 1 cut(s) 439
XmnI GAANNNNTTC 2 cut(s) 1202, 1920
XspI CTAG 3 cut(s) 732, 959, 1256
ZraI GACGTC 1 cut(s) 43
Zsp2I ATGCAT 1 cut(s) 1186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.