Rorug01G0026200

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0015798
Physical Location & Seq
Forward (+)
4470972 .. 4471931
960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0026200.1

Sequence Viewer

Length: 960 bp
ATGAAATCAGCTCTTGTTATTCTCTTCTTCTTCTGTTTGTCTGTTCACCAATCCCAAGGAGCATCTTTTTCACTCCCAAACCTAAACCATCATGAAATACCCATCCACGTACCACAGCCTGAAAGCATTGCTTTCGACTGCAATGGCGCTCCCTACGTGGGTGTGGCTGATGGCAGGATATTCAAGTGGCTAGGATACCCTTTTGGGTGGAGAGAATTCGCAGTCACCTCCCCCAATAGGCCGAGAGGAGTATGTGATTTAACAACCAACAAAGACAATGAACCGATATGCGGGAGGCCACTTGGTTTGAAATTTGACCTCACAACATGTGAACTGTATATCGCAGATGCCTATTTTGGACTTCTAAAGACAGGACCAAATGGTGGAGTAGCTCAAATACTTGCGACATCTGCTGAAGGAGTTCCTTTCCGTTTCTTAAACGGTGTCGACATTGATCGCCAAAGTGGAATGGTGTACTTCACAGACACCAGTGCTATTTACCAGAGAAGAAATTGGGAATTGTCAGTTAGTACAGGTGATAAAACTGGAAGGTTAATGCGTTATGATCCATGGACAAAGAATGTCACTGTGTTGCGTCATGACTTGTCTTTTGCAAATGGGGTGGCACTAAGCAAAGAAGGTGATTTTGTGCTAGTAACCCAAACCACCGCAAAGAACATTTTGAGGTACTGGCTTCGAGGGCCTAGAGCCAACACTGTTGATATTTTTTTCCAACTGCAAGGAGCCCCAGACAATATCATCCGCAACATAAATGGAGAATTTTGGGTTGCGGAAAATAGTGCTCGAAAAGGTAAAGCGGTCAAGCTTGACGCCCAAGGAAATTTTTTGGACGTGGTAGATGGACTCAACCCTGTGAGTCATGTTGAGCAGCGTTATAGAAACCTTTGGATAGGATCTGTGGTTAATAACTTTGTTGGGGTTATTCTTAATTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

319

Amino Acids

35.57

Weight (kDa)

8.24

Isoelectric Point (pI)

26.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 147 - 234 3.7e-29 Strictosidine synthase
SGL PF08450 178 - 287 4.2e-09 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015798)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01980 FvH4_7g01980
malus_domestica MD02G1298100.v1.1 MD07G1026000.v1.1
prunus_persica Prupe.2G026200_v2.0.a1
pyrus_communis pycom07g01980
rosa_chinensis RchiOBHm_Chr1g0318981
rosa_laevigata RLG00000030512
rosa_multiflora Rmu_sc0003503.1_g000001 Rmu_sc0006626.1_g000004
rosa_roxburghii Rroxscaffold_4G00328710
rosa_rugosa Rorug01G0026100 Rorug01G0026200
rosa_samantha Rh1AG038000
rosa_wichuraiana Rw1G002930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 383
AccI GTMKAC 1 cut(s) 447
AciI CCGC 5 cut(s) 291, 669, 763, 791, 818
AclWI GGATC 2 cut(s) 560, 922
AcsI RAATTY 4 cut(s) 215, 311, 779, 841
AcuI CTGAAG 1 cut(s) 435
AcyI GRCGYC 1 cut(s) 831
AfaI GTAC 4 cut(s) 111, 476, 532, 689
AfiI CCNNNNNNNGG 4 cut(s) 158, 237, 290, 383
AflIII ACRYGT 1 cut(s) 326
AgsI TTSAA 2 cut(s) 184, 310
AjiI CACGTC 1 cut(s) 853
AluBI AGCT 3 cut(s) 11, 392, 826
AluI AGCT 3 cut(s) 11, 392, 826
Alw21I GWGCWC 1 cut(s) 805
AlwI GGATC 2 cut(s) 560, 922
AoxI GGCC 3 cut(s) 239, 296, 701
ApeKI GCWGC 1 cut(s) 889
ApoI RAATTY 4 cut(s) 215, 311, 779, 841
ArsI GACNNNNNNTTYG 2 cut(s) 593, 625
Asp700I GAANNNNTTC 1 cut(s) 420
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 2 cut(s) 374, 701
AsuHPI GGTGA 4 cut(s) 38, 217, 548, 653
AvaII GGWCC 1 cut(s) 374
BanII GRGCYC 1 cut(s) 748
Bbv12I GWGCWC 1 cut(s) 805
BbvI GCAGC 1 cut(s) 901
BccI CCATC 4 cut(s) 96, 110, 164, 854
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciVI GTATCC 1 cut(s) 188
BfaI CTAG 3 cut(s) 191, 653, 705
BfoI RGCGCY 1 cut(s) 150
BfuI GTATCC 1 cut(s) 188
BisI GCNGC 1 cut(s) 890
BlsI GCNGC 1 cut(s) 891
Bme18I GGWCC 1 cut(s) 374
BmgBI CACGTC 1 cut(s) 853
BmgT120I GGNCC 2 cut(s) 374, 701
BmiI GGNNCC 1 cut(s) 745
BmsI GCATC 2 cut(s) 71, 337
BsaAI YACGTR 2 cut(s) 109, 157
BsaHI GRCGYC 1 cut(s) 831
BsaJI CCNNGG 3 cut(s) 55, 569, 835
Bsc4I CCNNNNNNNGG 4 cut(s) 158, 237, 290, 383
Bse1I ACTGG 3 cut(s) 489, 550, 695
Bse3DI GCAATG 2 cut(s) 126, 148
BseDI CCNNGG 3 cut(s) 55, 569, 835
BseGI GGATG 2 cut(s) 102, 759
BseLI CCNNNNNNNGG 4 cut(s) 158, 237, 290, 383
BseMI GCAATG 2 cut(s) 126, 148
BseNI ACTGG 3 cut(s) 489, 550, 695
BseRI GAGGAG 1 cut(s) 261
BseXI GCAGC 1 cut(s) 901
BshFI GGCC 3 cut(s) 241, 298, 703
BsiHKAI GWGCWC 1 cut(s) 805
BslI CCNNNNNNNGG 4 cut(s) 158, 237, 290, 383
BsnI GGCC 3 cut(s) 241, 298, 703
Bsp1286I GDGCHC 2 cut(s) 748, 805
Bsp143I GATC 3 cut(s) 454, 565, 914
Bsp19I CCATGG 1 cut(s) 569
BspACI CCGC 5 cut(s) 291, 669, 763, 791, 818
BspANI GGCC 3 cut(s) 241, 298, 703
BspHI TCATGA 2 cut(s) 91, 598
BspLI GGNNCC 1 cut(s) 745
BspPI GGATC 2 cut(s) 560, 922
BsrDI GCAATG 2 cut(s) 126, 148
BsrI ACTGG 3 cut(s) 489, 550, 695
BssECI CCNNGG 3 cut(s) 55, 569, 835
BssMI GATC 3 cut(s) 454, 565, 914
BssNI GRCGYC 1 cut(s) 831
BssT1I CCWWGG 3 cut(s) 55, 569, 835
Bst4CI ACNGT 4 cut(s) 336, 443, 589, 718
Bst6I CTCTTC 1 cut(s) 29
BstACI GRCGYC 1 cut(s) 831
BstBAI YACGTR 2 cut(s) 109, 157
BstDEI CTNAG 1 cut(s) 629
BstDSI CCRYGG 1 cut(s) 569
BstF5I GGATG 2 cut(s) 102, 759
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 3 cut(s) 457, 568, 917
BstMBI GATC 3 cut(s) 454, 565, 914
BstMWI GCNNNNNNNGC 2 cut(s) 410, 700
BstNSI RCATGY 1 cut(s) 330
BstV1I GCAGC 1 cut(s) 901
BstX2I RGATCY 1 cut(s) 914
BstYI RGATCY 1 cut(s) 914
BsuI GTATCC 1 cut(s) 188
BsuRI GGCC 3 cut(s) 241, 298, 703
BtgI CCRYGG 1 cut(s) 569
BtrI CACGTC 1 cut(s) 853
BtsCI GGATG 2 cut(s) 102, 759
BtsIMutI CAGTG 3 cut(s) 496, 585, 714
CciI TCATGA 2 cut(s) 91, 598
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 374, 701
CseI GACGC 2 cut(s) 584, 839
Csp6I GTAC 4 cut(s) 110, 475, 531, 688
CspCI CAANNNNNGTGG 4 cut(s) 288, 323, 603, 638
CviAII CATG 5 cut(s) 92, 327, 570, 599, 881
CviQI GTAC 4 cut(s) 110, 475, 531, 688
DdeI CTNAG 1 cut(s) 629
DpnI GATC 3 cut(s) 456, 567, 916
DpnII GATC 3 cut(s) 454, 565, 914
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco130I CCWWGG 3 cut(s) 55, 569, 835
Eco24I GRGCYC 1 cut(s) 748
Eco47I GGWCC 1 cut(s) 374
Eco57I CTGAAG 1 cut(s) 435
EcoO109I RGGNCCY 1 cut(s) 701
EcoRI GAATTC 1 cut(s) 215
EcoT14I CCWWGG 3 cut(s) 55, 569, 835
EcoT38I GRGCYC 1 cut(s) 748
ErhI CCWWGG 3 cut(s) 55, 569, 835
FaeI CATG 5 cut(s) 95, 330, 573, 602, 884
FalI AAGNNNNNCTT 2 cut(s) 115, 147
FatI CATG 5 cut(s) 91, 326, 569, 598, 880
FauI CCCGC 1 cut(s) 284
FblI GTMKAC 1 cut(s) 447
Fnu4HI GCNGC 1 cut(s) 890
FokI GGATG 2 cut(s) 89, 746
FriOI GRGCYC 1 cut(s) 748
Fsp4HI GCNGC 1 cut(s) 890
FspBI CTAG 3 cut(s) 191, 653, 705
GlaI GCGC 1 cut(s) 148
GluI GCNGC 1 cut(s) 890
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 3 cut(s) 241, 298, 703
HgaI GACGC 2 cut(s) 584, 839
HhaI GCGC 1 cut(s) 149
Hin1I GRCGYC 1 cut(s) 831
Hin1II CATG 5 cut(s) 95, 330, 573, 602, 884
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HindIII AAGCTT 1 cut(s) 824
HinfI GANTC 2 cut(s) 864, 877
HphI GGTGA 4 cut(s) 38, 217, 548, 653
Hpy166II GTNNAC 4 cut(s) 46, 332, 448, 475
Hpy188III TCNNGA 2 cut(s) 92, 599
Hpy8I GTNNAC 4 cut(s) 46, 332, 448, 475
HpyAV CCTTC 3 cut(s) 410, 543, 632
HpyCH4III ACNGT 4 cut(s) 336, 443, 589, 718
HpyCH4IV ACGT 3 cut(s) 108, 156, 852
HpyCH4V TGCA 3 cut(s) 141, 614, 739
HpyF10VI GCNNNNNNNGC 2 cut(s) 410, 700
HpyF3I CTNAG 1 cut(s) 629
HpySE526I ACGT 3 cut(s) 108, 156, 852
Hsp92I GRCGYC 1 cut(s) 831
Hsp92II CATG 5 cut(s) 95, 330, 573, 602, 884
HspAI GCGC 1 cut(s) 147
Kzo9I GATC 3 cut(s) 454, 565, 914
LmnI GCTCC 3 cut(s) 59, 154, 743
Lsp1109I GCAGC 1 cut(s) 901
LweI GCATC 2 cut(s) 71, 337
MaeI CTAG 3 cut(s) 191, 653, 705
MaeII ACGT 3 cut(s) 108, 156, 852
MaeIII GTNAC 3 cut(s) 223, 583, 655
MalI GATC 3 cut(s) 456, 567, 916
MboI GATC 3 cut(s) 454, 565, 914
MboII GAAGA 4 cut(s) 16, 19, 22, 519
MflI RGATCY 1 cut(s) 914
MhlI GDGCHC 2 cut(s) 748, 805
MluCI AATT 7 cut(s) 215, 311, 511, 518, 779, 841, 949
MlyI GAGTC 2 cut(s) 858, 886
MmeI TCCRAC 1 cut(s) 757
MnlI CCTC 6 cut(s) 238, 239, 288, 329, 678, 692
MroXI GAANNNNTTC 1 cut(s) 420
MseI TTAA 5 cut(s) 260, 437, 554, 924, 948
MwoI GCNNNNNNNGC 2 cut(s) 410, 700
NcoI CCATGG 1 cut(s) 569
NdeII GATC 3 cut(s) 454, 565, 914
NlaIII CATG 5 cut(s) 95, 330, 573, 602, 884
NlaIV GGNNCC 1 cut(s) 745
NmeAIII GCCGAG 1 cut(s) 267
NmuCI GTSAC 2 cut(s) 223, 583
NspI RCATGY 1 cut(s) 330
PagI TCATGA 2 cut(s) 91, 598
PciI ACATGT 1 cut(s) 326
PdmI GAANNNNTTC 1 cut(s) 420
PflMI CCANNNNNTGG 1 cut(s) 383
PkrI GCNGC 1 cut(s) 891
PleI GAGTC 2 cut(s) 858, 885
PpsI GAGTC 2 cut(s) 858, 885
Ppu21I YACGTR 2 cut(s) 109, 157
PscI ACATGT 1 cut(s) 326
PspN4I GGNNCC 1 cut(s) 745
PspPI GGNCC 2 cut(s) 374, 701
PsuI RGATCY 1 cut(s) 914
RsaI GTAC 4 cut(s) 111, 476, 532, 689
RsaNI GTAC 4 cut(s) 110, 475, 531, 688
SalI GTCGAC 1 cut(s) 446
SaqAI TTAA 5 cut(s) 260, 437, 554, 924, 948
SatI GCNGC 1 cut(s) 890
Sau3AI GATC 3 cut(s) 454, 565, 914
Sau96I GGNCC 2 cut(s) 374, 701
SchI GAGTC 2 cut(s) 858, 886
SduI GDGCHC 2 cut(s) 748, 805
SfaNI GCATC 2 cut(s) 71, 337
SinI GGWCC 1 cut(s) 374
Sse9I AATT 7 cut(s) 215, 311, 511, 518, 779, 841, 949
SsiI CCGC 5 cut(s) 291, 669, 763, 791, 818
SspMI CTAG 3 cut(s) 191, 653, 705
StyI CCWWGG 3 cut(s) 55, 569, 835
TaaI ACNGT 4 cut(s) 336, 443, 589, 718
TaiI ACGT 3 cut(s) 111, 159, 855
TaqI TCGA 4 cut(s) 135, 447, 697, 805
TasI AATT 7 cut(s) 215, 311, 511, 518, 779, 841, 949
TatI WGTACW 2 cut(s) 474, 530
Tru1I TTAA 5 cut(s) 260, 437, 554, 924, 948
Tru9I TTAA 5 cut(s) 260, 437, 554, 924, 948
TscAI CASTG 3 cut(s) 496, 592, 721
TseFI GTSAC 2 cut(s) 223, 583
TseI GCWGC 1 cut(s) 889
Tsp45I GTSAC 2 cut(s) 223, 583
TspDTI ATGAA 3 cut(s) 17, 108, 294
TspGWI ACGGA 1 cut(s) 419
TspRI CASTG 3 cut(s) 496, 592, 721
Van91I CCANNNNNTGG 1 cut(s) 383
VpaK11BI GGWCC 1 cut(s) 374
XapI RAATTY 4 cut(s) 215, 311, 779, 841
XceI RCATGY 1 cut(s) 330
XmiI GTMKAC 1 cut(s) 447
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 3 cut(s) 191, 653, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.