MD02G1298100.v1.1

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0015798
Physical Location & Seq
Reverse (-)
35295674 .. 35301462
5789 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1298100.v1.1.491

Sequence Viewer

Length: 3327 bp
ATGAGAGAGAAAATGGAGGAGTACTTGCGGAGGAACTTCGACGTCGAACCTAAACGCCCGTCCGAGGAGGCCCTGAAGCGGTGGAGGTCCGCCGTCTCCGTCATCAAGAACCCTACCCGAAGGTTCCGCATGGTCGCTGATCTCGCCAAGCGCGCCGAGGACAAGCGGAAGCGCAAGAGCCTCCAGGAGAAGATACGTGTTGCTATATACGTACAAAAAGCAGCCTTGCAATTCATTGACGCTGGTAATCGCGGGGATTATAATCTTCCCAAAGAAGTTAAAGATGCTGGTTTTGGCATTGATCCTGAGGAAATTGCATCCTTTGCTCGATCTCATGATACCAAAGGGTTGGAAGGACATGGAGGAGTATCAGGATTAGCAGGAGAGGTTTCTGTATCGCTGGAAGATGGTGTTGTTTCAAGCAAGATACCTATTAGGCAGGATATATATGGCTTCAACCAATATGCAGAGAAACCTTCTAAAGGTTTTGGGATGTATCTCTGGGAAGCCGTACAAGATTTAACTCTAATCATCCTCATGGTTGCTGCTGCGGTCTCTATAGGTGTTGGAATCGCAACTGAAGGAATGCCGAAAGGTATATATGATGGCTTGGGAATAATACTTAGCATATTGCTAGTAGTGTTAGTGACTGCAATCAGTGACTACAAGCAGTCATTGCAATTTAAAGAACTGGACAAAGAGAAGAAAAACATAATTGTTCAGGTGACTAGAGACGGATGCAGACAGAAGGTCTCTATCTACGATTTGGTGGTTGGAGACATTGTTCATCTTTCCATCGGCGATCAAGTTCCAGCTGATGGTATTTTCGTATCAGGCCACAGCTTACAAGTCGACGAATCAAGCTTGTCAGGTGAGAGTGAGCCAGCGGACATAAATCAAGAGAGTCCCTTTCTTCTTGCAGGAACCAAAGTGCAAGATGGTTCTGGGAAAATGTTGGTGACATCAGTTGGTATGAGGACCGAATGGGGACGACTAATGGTTGCTCTGAGTGAAGGGGGAAACAATGAGACACCACTACAGGTAAAACTTAATGGAGTTGCAACCATTATCGGAAAAATTGGTTTGGCTTTCGCGGTGCTAACATTTATAGTTCTGACTTCAAGGTTTTTAATAAACAAGGCGGTTCACCATCACATGACAGACTGGTCTTCCAAAGATGCATTAAAGCTTTTGAATTACTTCTCTACTGCCGTCATTATAATTGTTGTTGCAGTTCCGGAAGGGTTACCCCTGGCAGTGACACTAAGTCTCGCCTTTGCAATGAAAAAACTCATGTTCGATAAAGCACTTGTGAGGCATCTCTCTGCATGTGAGACAATGGGTTCTGCTACTTGTATTTGCACAGATAAAACAGGAACCCTGACAACAAATCATATGGTTGTGAACAAGATATGGGTCTGTAATGAAACCGAGACTATAACGAGTAATGATGACAATGACGTGTTGAAGTCGGCTTCTGAAGGAGTACATGACCTCCTTTTACAGTCCATCTTTCTGAATACAAGCGCGGAGGTAGTCAAGGGGGAAGATGGAAAGAATAGCATTATGGGGACTCCAACAGAGACGGCGGTATTTGAATTTGGAATGCTTCTTGGAGGTGATTTTAAATCTTACAGTGAAGATTATAAGATACTGACGGTTGAACCATTCAATTCAGTCAGAAAAAAGATGTCGGTGCTAGTGGCTCTGCCTGATGGAGGAAAGAAACGAGCATTCTGCAAAGGAGCATCAGAAATTATCTTAAAAATGTGTGATAATGTTGTTGCCACTCATGGTGAAGCTGTGCCTCTATCGGACGAGCAAAGAACAAATATCTCAGATGTCATAAATGGTTTTGCTTGTGAAGCTTTAAGAACTCTATGCTTAGCTTTCAAGAATGTATACGACTCCTCTAGTGAAGACAGTATTCCTGACGATAAATATACGTTAATCGCTGTTGTTGGAATCAAGGATCCTGTGCGTCCGGGGGTGAGGGAAGCCGTGAAGACTTGTCTAGATGCTGGGATTACTGTAAGGATGGTCACTGGTGATAATATTAATACGGCTAAAGCCATAGCTAAAGAGTGTGGCATTTTGACAGAAGATGGTTTGGCTATAGAAGGGCCAGATTTCCAAAATAAGAGCTCGCAGGAGATGGCAGAAATAATTCCGAAACTGCAGGTAATGGCTCGATCGTTGCCTTTGGACAAGCACACCTTGGTGAAACAGTTGAGGAATGAGCTTAGAGAAGTTGTGGCAGTAACTGGTGATGGGGCGAATGACGCTCCTGCACTGCACGAGGCAGACATCGGACTTGCTATGGGTATTGCAGGAACGGAGGTTGCGAAAGAAAGTGCTGATGTTATAATAATGGATGACAACTTCACAAGCATAGTGAATGTTGCACGATGGGGTCGGGCGGTTTATGTCAACATTCAAAAGTTTGTACAGTTCCAGCTAACAGTTAACATCGTCGCTCTGATGCTCAGTTTCATTTCAGCATGCGTCTCAGGAACCGCTCCTCTCACGGCTGTGCAGATGCTTTGGGTGAACTTGATCATGGACACTCTTGGTGCTTTGGCGCTGGCTACAGAGCCCCCAAACGAGGGTCTAATGAGAAGACCACCGGTCGGAAGGAACATCAAGTTCATCACCGGGATCATGTGGAGGAACATCATTGGCCAGAGTATCTACCAGATTGCTGTCCTTTTGGCTCTCAAATTTTGTGGAATCCGGCTTTTGAAGCTCACCGGTTCAGATGCGAATTCCGTTCTCAATACCGTTGTCTTCAACTCTTTTGTGTTCTGCCAGGTATTCAACGAGATAAACAGCCGTGATATGGAGAAGATCAATGTGTTCAGTGGCATGTTCAGCAGTTATACCTTCATGATGGTGATGATCTCCACCGTTGTTTCCCAGATCATCATAGTTGAATTCTTGGGCAAGTTTGCACAAACCGTGCCACTGAGCTGGAAGTTGTGGTTAACAAGCGTCTTGATCGGCGCTGTAAGTTTGCTCGTCGCTGTCGTCCTCAAGTTCATTCCTGTTTCAGTCAGGAAACAAGAAGCTTCCCGGGATCACGGGGATGTCTCTACGAACCGCTCCTCCGTGGCCCTGACCTTGCATGAAAGATCGAAACTGCTTGTTCGTGAAAGATTTTTGTGCAGAATTTTGGAGGAATCTCTTCGTTTTTTTCGTTGGATGTTTGCGCAACATTTTTTCGACACCATTTCTCTCGTCATTTTTTGTTGTACATTTCAGTGTTCATTGAGGTCAGCGAGAAATCAGTCTCCTTTTGGCCATTTCGGTGGTATTTATACGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1109

Amino Acids

121.57

Weight (kDa)

6.54

Isoelectric Point (pI)

40.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaATP_NAI PF12515 10 - 53 9.3e-20 Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N PF00690 120 - 186 1.8e-09 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 238 - 333 6.3e-22 P-type ATPase actuator domain
Cation_ATPase PF13246 500 - 662 2.6e-33 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 642 - 769 6.7e-13 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 840 - 1014 1.5e-40 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015798)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01980 FvH4_7g01980
malus_domestica MD02G1298100.v1.1 MD07G1026000.v1.1
prunus_persica Prupe.2G026200_v2.0.a1
pyrus_communis pycom07g01980
rosa_chinensis RchiOBHm_Chr1g0318981
rosa_laevigata RLG00000030512
rosa_multiflora Rmu_sc0003503.1_g000001 Rmu_sc0006626.1_g000004
rosa_roxburghii Rroxscaffold_4G00328710
rosa_rugosa Rorug01G0026100 Rorug01G0026200
rosa_samantha Rh1AG038000
rosa_wichuraiana Rw1G002930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 261, 1220, 1647, 2366
AasI GACNNNNNNGTC 1 cut(s) 1165
AatII GACGTC 1 cut(s) 45
Acc16I TGCGCA 1 cut(s) 3210
Acc36I ACCTGC 1 cut(s) 2170
AccB7I CCANNNNNTGG 1 cut(s) 818
AccBSI CCGCTC 2 cut(s) 2519, 3102
AccI GTMKAC 2 cut(s) 852, 1902
AccII CGCG 4 cut(s) 153, 252, 1094, 1529
AccIII TCCGGA 1 cut(s) 1237
AclWI GGATC 5 cut(s) 296, 1967, 1980, 2666, 3084
AcoI YGGCCR 2 cut(s) 2680, 3298
AcsI RAATTY 5 cut(s) 1598, 2720, 2764, 2933, 3168
AcuI CTGAAG 3 cut(s) 95, 600, 1500
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 6 cut(s) 23, 213, 513, 1488, 2448, 3253
AfiI CCNNNNNNNGG 9 cut(s) 64, 78, 482, 818, 1718, 2605, 2606, 2630, 2839
AflIII ACRYGT 2 cut(s) 196, 1461
AgeI ACCGGT 2 cut(s) 2626, 2750
AhdI GACNNNNNGTC 3 cut(s) 749, 1266, 2627
AjiI CACGTC 1 cut(s) 1462
AjnI CCWGG 3 cut(s) 183, 1251, 2808
AleI CACNNNNGTG 2 cut(s) 2219, 2531
AloI GAACNNNNNNTCC 4 cut(s) 768, 800, 3089, 3121
Alw21I GWGCWC 1 cut(s) 2147
AlwI GGATC 5 cut(s) 296, 1967, 1980, 2666, 3084
AlwNI CAGNNNCTG 1 cut(s) 2264
Ama87I CYCGRG 1 cut(s) 3072
Aor13HI TCCGGA 1 cut(s) 1237
AoxI GGCC 6 cut(s) 69, 835, 2123, 2680, 3111, 3298
ApeKI GCWGC 3 cut(s) 221, 545, 548
ApoI RAATTY 5 cut(s) 1598, 2720, 2764, 2933, 3168
AseI ATTAAT 1 cut(s) 2058
AsiGI ACCGGT 2 cut(s) 2626, 2750
Asp700I GAANNNNTTC 3 cut(s) 1199, 2166, 3183
AspLEI GCGC 7 cut(s) 153, 155, 174, 1529, 2584, 3005, 3211
AspS9I GGNCC 5 cut(s) 70, 87, 978, 2123, 3112
AsuC2I CCSGG 4 cut(s) 1986, 2656, 3073, 3074
AvaI CYCGRG 1 cut(s) 3072
AvaII GGWCC 2 cut(s) 87, 978
AxyI CCTNAGG 1 cut(s) 306
BalI TGGCCA 2 cut(s) 2682, 3300
BamHI GGATCC 1 cut(s) 1972
BanII GRGCYC 2 cut(s) 2147, 2598
BauI CACGAG 1 cut(s) 2297
BbsI GAAGAC 5 cut(s) 1161, 1926, 2012, 2626, 2779
Bbv12I GWGCWC 1 cut(s) 2147
BbvI GCAGC 3 cut(s) 233, 532, 535
BceAI ACGGC 8 cut(s) 77, 494, 1196, 1602, 1985, 2079, 2544, 2817
BcgI CGANNNNNNTGC 2 cut(s) 1719, 1753
BciT130I CCWGG 3 cut(s) 185, 1253, 2810
BclI TGATCA 1 cut(s) 2556
BcnI CCSGG 4 cut(s) 1986, 2656, 3073, 3074
BfaI CTAG 5 cut(s) 635, 729, 1700, 1914, 2015
BfmI CTRYAG 5 cut(s) 558, 1037, 2115, 2177, 2589
BfoI RGCGCY 2 cut(s) 2585, 3006
BfuAI ACCTGC 1 cut(s) 2170
BisI GCNGC 3 cut(s) 222, 546, 549
BlpI GCTNAGC 1 cut(s) 1885
BlsI GCNGC 3 cut(s) 223, 547, 550
BmcAI AGTACT 1 cut(s) 23
Bme1390I CCNGG 7 cut(s) 185, 1253, 1986, 2656, 2810, 3073, 3074
Bme18I GGWCC 2 cut(s) 87, 978
BmeRI GACNNNNNGTC 3 cut(s) 749, 1266, 2627
BmeT110I CYCGRG 1 cut(s) 3072
BmgBI CACGTC 1 cut(s) 1462
BmgT120I GGNCC 5 cut(s) 70, 87, 978, 2123, 3112
BmiI GGNNCC 5 cut(s) 125, 925, 1378, 1974, 2515
BmrFI CCNGG 7 cut(s) 185, 1253, 1986, 2656, 2810, 3073, 3074
BpiI GAAGAC 5 cut(s) 1161, 1926, 2012, 2626, 2779
BpmI CTGGAG 1 cut(s) 167
Bpu1102I GCTNAGC 1 cut(s) 1885
BpuEI CTTGAG 1 cut(s) 3017
BpuMI CCSGG 4 cut(s) 1986, 2656, 3073, 3074
BsaAI YACGTR 2 cut(s) 197, 211
BsaBI GATNNNNATC 1 cut(s) 261
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 2 cut(s) 559, 757
BsaJI CCNNGG 7 cut(s) 63, 156, 1251, 1985, 2217, 3072, 3108
BsaWI WCCGGW 3 cut(s) 1237, 2626, 2750
BsaXI ACNNNNNCTCC 6 cut(s) 768, 798, 1479, 1509, 3089, 3119
Bsc4I CCNNNNNNNGG 9 cut(s) 64, 78, 482, 818, 1718, 2605, 2606, 2630, 2839
Bse118I RCCGGY 2 cut(s) 2626, 2750
Bse1I ACTGG 4 cut(s) 696, 1169, 2050, 2269
Bse21I CCTNAGG 1 cut(s) 306
Bse3DI GCAATG 2 cut(s) 674, 1287
Bse8I GATNNNNATC 1 cut(s) 261
BseAI TCCGGA 1 cut(s) 1237
BseBI CCWGG 3 cut(s) 185, 1253, 2810
BseDI CCNNGG 7 cut(s) 63, 156, 1251, 1985, 2217, 3072, 3108
BseGI GGATG 8 cut(s) 317, 498, 531, 743, 2043, 2380, 3091, 3207
BseJI GATNNNNATC 1 cut(s) 261
BseLI CCNNNNNNNGG 9 cut(s) 64, 78, 482, 818, 1718, 2605, 2606, 2630, 2839
BseMI GCAATG 2 cut(s) 674, 1287
BseMII CTCAG 6 cut(s) 297, 998, 1851, 2500, 2523, 2957
BseNI ACTGG 4 cut(s) 696, 1169, 2050, 2269
BsePI GCGCGC 1 cut(s) 151
BseRI GAGGAG 6 cut(s) 32, 80, 378, 1900, 2511, 3094
BseXI GCAGC 3 cut(s) 233, 532, 535
BseYI CCCAGC 1 cut(s) 2021
BsgI GTGCAG 4 cut(s) 2274, 2279, 2555, 3184
Bsh1236I CGCG 4 cut(s) 153, 252, 1094, 1529
Bsh1285I CGRYCG 2 cut(s) 2195, 2631
BshFI GGCC 6 cut(s) 71, 837, 2125, 2682, 3113, 3300
BshTI ACCGGT 2 cut(s) 2626, 2750
BsiEI CGRYCG 2 cut(s) 2195, 2631
BsiHKAI GWGCWC 1 cut(s) 2147
BsiHKCI CYCGRG 1 cut(s) 3072
BsiSI CCGG 7 cut(s) 1238, 1985, 2627, 2655, 2734, 2751, 3073
BslFI GGGAC 3 cut(s) 891, 1002, 1585
BslI CCNNNNNNNGG 9 cut(s) 64, 78, 482, 818, 1718, 2605, 2606, 2630, 2839
BsmBI CGTCTC 4 cut(s) 100, 726, 1577, 2512
BsmFI GGGAC 3 cut(s) 891, 1002, 1585
BsmI GAATGC 3 cut(s) 591, 1611, 1733
BsnI GGCC 6 cut(s) 71, 837, 2125, 2682, 3113, 3300
Bso31I GGTCTC 2 cut(s) 559, 757
BsoBI CYCGRG 1 cut(s) 3072
Bsp1286I GDGCHC 2 cut(s) 2147, 2598
Bsp13I TCCGGA 1 cut(s) 1237
Bsp1407I TGTACA 2 cut(s) 2446, 3251
Bsp1720I GCTNAGC 1 cut(s) 1885
BspANI GGCC 6 cut(s) 71, 837, 2125, 2682, 3113, 3300
BspCNI CTCAG 6 cut(s) 298, 999, 1850, 2499, 2522, 2958
BspEI TCCGGA 1 cut(s) 1237
BspFNI CGCG 4 cut(s) 153, 252, 1094, 1529
BspHI TCATGA 2 cut(s) 334, 2886
BspLI GGNNCC 5 cut(s) 125, 925, 1378, 1974, 2515
BspMAI CTGCAG 1 cut(s) 2181
BspMI ACCTGC 1 cut(s) 2170
BspPI GGATC 5 cut(s) 296, 1967, 1980, 2666, 3084
BspTNI GGTCTC 2 cut(s) 559, 757
BsrBI CCGCTC 2 cut(s) 2519, 3102
BsrDI GCAATG 2 cut(s) 674, 1287
BsrFI RCCGGY 2 cut(s) 2626, 2750
BsrGI TGTACA 2 cut(s) 2446, 3251
BsrI ACTGG 4 cut(s) 696, 1169, 2050, 2269
BssAI RCCGGY 2 cut(s) 2626, 2750
BssECI CCNNGG 7 cut(s) 63, 156, 1251, 1985, 2217, 3072, 3108
BssHII GCGCGC 1 cut(s) 151
BssNAI GTATAC 1 cut(s) 1903
BssNI GRCGYC 1 cut(s) 42
BssSI CACGAG 1 cut(s) 2297
BssT1I CCWWGG 1 cut(s) 2217
Bst1107I GTATAC 1 cut(s) 1903
Bst2BI CACGAG 1 cut(s) 2297
Bst2UI CCWGG 3 cut(s) 185, 1253, 2810
Bst6I CTCTTC 1 cut(s) 3189
BstACI GRCGYC 1 cut(s) 42
BstAPI GCANNNNNTGC 2 cut(s) 323, 676
BstAUI TGTACA 2 cut(s) 2446, 3251
BstBAI YACGTR 2 cut(s) 197, 211
BstC8I GCNNGC 5 cut(s) 153, 885, 2147, 2503, 2586
BstDSI CCRYGG 1 cut(s) 3108
BstEII GGTNACC 1 cut(s) 1245
BstENI CCTNNNNNAGG 2 cut(s) 480, 1716
BstF5I GGATG 8 cut(s) 317, 498, 531, 743, 2043, 2380, 3091, 3207
BstFNI CGCG 4 cut(s) 153, 252, 1094, 1529
BstH2I RGCGCY 2 cut(s) 2585, 3006
BstHHI GCGC 7 cut(s) 153, 155, 174, 1529, 2584, 3005, 3211
BstMCI CGRYCG 2 cut(s) 2195, 2631
BstMWI GCNNNNNNNGC 8 cut(s) 143, 152, 323, 676, 1865, 2282, 2743, 2871
BstNI CCWGG 3 cut(s) 185, 1253, 2810
BstNSI RCATGY 3 cut(s) 1332, 2505, 2869
BstPI GGTNACC 1 cut(s) 1245
BstSCI CCNGG 7 cut(s) 183, 1251, 1984, 2654, 2808, 3071, 3072
BstSFI CTRYAG 5 cut(s) 558, 1037, 2115, 2177, 2589
BstSNI TACGTA 1 cut(s) 211
BstUI CGCG 4 cut(s) 153, 252, 1094, 1529
BstV1I GCAGC 3 cut(s) 233, 532, 535
BstV2I GAAGAC 5 cut(s) 1161, 1926, 2012, 2626, 2779
BstX2I RGATCY 1 cut(s) 1972
BstXI CCANNNNNNTGG 3 cut(s) 349, 2970, 3308
BstYI RGATCY 1 cut(s) 1972
BstZ17I GTATAC 1 cut(s) 1903
Bsu36I CCTNAGG 1 cut(s) 306
BsuRI GGCC 6 cut(s) 71, 837, 2125, 2682, 3113, 3300
BtgI CCRYGG 1 cut(s) 3108
BtrI CACGTC 1 cut(s) 1462
BtsCI GGATG 8 cut(s) 317, 498, 531, 743, 2043, 2380, 3091, 3207
BtsI GCAGTG 2 cut(s) 1263, 2291
BtsIMutI CAGTG 8 cut(s) 664, 1263, 1642, 2043, 2291, 2866, 2963, 3266
BveI ACCTGC 1 cut(s) 2170
Cac8I GCNNGC 5 cut(s) 153, 885, 2147, 2503, 2586
CaiI CAGNNNCTG 1 cut(s) 2264
CciI TCATGA 2 cut(s) 334, 2886
CfoI GCGC 7 cut(s) 153, 155, 174, 1529, 2584, 3005, 3211
Cfr10I RCCGGY 2 cut(s) 2626, 2750
Cfr13I GGNCC 5 cut(s) 70, 87, 978, 2123, 3112
Cfr9I CCCGGG 1 cut(s) 3072
CseI GACGC 5 cut(s) 248, 1970, 2291, 2494, 2980
Csp6I GTAC 6 cut(s) 22, 212, 512, 1487, 2447, 3252
CspAI ACCGGT 2 cut(s) 2626, 2750
CspCI CAANNNNNGTGG 2 cut(s) 2707, 2742
CviQI GTAC 6 cut(s) 22, 212, 512, 1487, 2447, 3252
DraI TTTAAA 2 cut(s) 685, 1627
DrdI GACNNNNNNGTC 1 cut(s) 1165
DriI GACNNNNNGTC 3 cut(s) 749, 1266, 2627
DseDI GACNNNNNNGTC 1 cut(s) 1165
EaeI YGGCCR 2 cut(s) 2680, 3298
Eam1104I CTCTTC 1 cut(s) 3189
Eam1105I GACNNNNNGTC 3 cut(s) 749, 1266, 2627
EarI CTCTTC 1 cut(s) 3189
EciI GGCGGA 1 cut(s) 79
Ecl136II GAGCTC 1 cut(s) 2145
Eco105I TACGTA 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 2217
Eco24I GRGCYC 2 cut(s) 2147, 2598
Eco31I GGTCTC 2 cut(s) 559, 757
Eco47I GGWCC 2 cut(s) 87, 978
Eco53kI GAGCTC 1 cut(s) 2145
Eco57I CTGAAG 3 cut(s) 95, 600, 1500
Eco81I CCTNAGG 1 cut(s) 306
Eco88I CYCGRG 1 cut(s) 3072
Eco91I GGTNACC 1 cut(s) 1245
EcoICRI GAGCTC 1 cut(s) 2145
EcoNI CCTNNNNNAGG 2 cut(s) 480, 1716
EcoO109I RGGNCCY 1 cut(s) 70
EcoO65I GGTNACC 1 cut(s) 1245
EcoRI GAATTC 2 cut(s) 2764, 2933
EcoRII CCWGG 3 cut(s) 183, 1251, 2808
EcoT14I CCWWGG 1 cut(s) 2217
EcoT22I ATGCAT 1 cut(s) 1183
EcoT38I GRGCYC 2 cut(s) 2147, 2598
ErhI CCWWGG 1 cut(s) 2217
Esp3I CGTCTC 4 cut(s) 100, 726, 1577, 2512
FalI AAGNNNNNCTT 2 cut(s) 2201, 2233
FaqI GGGAC 3 cut(s) 891, 1002, 1585
FauI CCCGC 1 cut(s) 245
FauNDI CATATG 1 cut(s) 1395
FbaI TGATCA 1 cut(s) 2556
FblI GTMKAC 2 cut(s) 852, 1902
Fnu4HI GCNGC 3 cut(s) 222, 546, 549
FokI GGATG 8 cut(s) 304, 505, 518, 750, 2050, 2387, 3098, 3214
FriOI GRGCYC 2 cut(s) 2147, 2598
Fsp4HI GCNGC 3 cut(s) 222, 546, 549
FspBI CTAG 5 cut(s) 635, 729, 1700, 1914, 2015
FspI TGCGCA 1 cut(s) 3210
GlaI GCGC 7 cut(s) 152, 154, 173, 1528, 2583, 3004, 3210
GluI GCNGC 3 cut(s) 222, 546, 549
GsaI CCCAGC 1 cut(s) 2025
GsuI CTGGAG 1 cut(s) 167
HaeII RGCGCY 2 cut(s) 2585, 3006
HaeIII GGCC 6 cut(s) 71, 837, 2125, 2682, 3113, 3300
HapII CCGG 7 cut(s) 1238, 1985, 2627, 2655, 2734, 2751, 3073
HgaI GACGC 5 cut(s) 248, 1970, 2291, 2494, 2980
HhaI GCGC 7 cut(s) 153, 155, 174, 1529, 2584, 3005, 3211
Hin1I GRCGYC 1 cut(s) 42
Hin6I GCGC 7 cut(s) 151, 153, 172, 1527, 2582, 3003, 3209
HinP1I GCGC 7 cut(s) 151, 153, 172, 1527, 2582, 3003, 3209
HincII GTYRAC 4 cut(s) 853, 2431, 2467, 2985
HindII GTYRAC 4 cut(s) 853, 2431, 2467, 2985
HindIII AAGCTT 4 cut(s) 862, 1187, 1866, 3066
HinfI GANTC 8 cut(s) 570, 857, 904, 1573, 1907, 1965, 2730, 3179
HpaI GTTAAC 2 cut(s) 2467, 2985
HpaII CCGG 7 cut(s) 1238, 1985, 2627, 2655, 2734, 2751, 3073
Hpy166II GTNNAC 8 cut(s) 853, 1147, 1405, 1903, 2431, 2467, 2551, 2985
Hpy8I GTNNAC 8 cut(s) 853, 1147, 1405, 1903, 2431, 2467, 2551, 2985
Hpy99I CGWCG 5 cut(s) 44, 47, 857, 2477, 3023
HpyCH4IV ACGT 5 cut(s) 42, 196, 210, 1461, 1946
HpyF10VI GCNNNNNNNGC 8 cut(s) 143, 152, 323, 676, 1865, 2282, 2743, 2871
HpySE526I ACGT 5 cut(s) 42, 196, 210, 1461, 1946
Hsp92I GRCGYC 1 cut(s) 42
HspAI GCGC 7 cut(s) 151, 153, 172, 1527, 2582, 3003, 3209
Kpn2I TCCGGA 1 cut(s) 1237
Ksp22I TGATCA 1 cut(s) 2556
KspAI GTTAAC 2 cut(s) 2467, 2985
LmnI GCTCC 4 cut(s) 1745, 2290, 2524, 3107
Lsp1109I GCAGC 3 cut(s) 233, 532, 535
MaeI CTAG 5 cut(s) 635, 729, 1700, 1914, 2015
MaeII ACGT 5 cut(s) 42, 196, 210, 1461, 1946
MaeIII GTNAC 8 cut(s) 646, 659, 724, 958, 1245, 1258, 2041, 2260
MbiI CCGCTC 2 cut(s) 2519, 3102
MflI RGATCY 1 cut(s) 1972
MhlI GDGCHC 2 cut(s) 2147, 2598
MlsI TGGCCA 2 cut(s) 2682, 3300
MluNI TGGCCA 2 cut(s) 2682, 3300
MlyI GAGTC 3 cut(s) 913, 1567, 1901
MmeI TCCRAC 7 cut(s) 330, 547, 754, 1601, 1942, 2611, 3179
Mox20I TGGCCA 2 cut(s) 2682, 3300
Mph1103I ATGCAT 1 cut(s) 1183
MroI TCCGGA 1 cut(s) 1237
MroXI GAANNNNTTC 3 cut(s) 1199, 2166, 3183
MscI TGGCCA 2 cut(s) 2682, 3300
MslI CAYNNNNRTG 7 cut(s) 536, 2219, 2531, 2891, 3084, 3259, 3306
Msp20I TGGCCA 2 cut(s) 2682, 3300
MspA1I CMGCKG 2 cut(s) 815, 887
MspI CCGG 7 cut(s) 1238, 1985, 2627, 2655, 2734, 2751, 3073
MspR9I CCNGG 7 cut(s) 185, 1253, 1986, 2656, 2810, 3073, 3074
Mva1269I GAATGC 3 cut(s) 591, 1611, 1733
MvaI CCWGG 3 cut(s) 185, 1253, 2810
MvnI CGCG 4 cut(s) 153, 252, 1094, 1529
MwoI GCNNNNNNNGC 8 cut(s) 143, 152, 323, 676, 1865, 2282, 2743, 2871
NciI CCSGG 4 cut(s) 1986, 2656, 3073, 3074
NdeI CATATG 1 cut(s) 1395
NlaIV GGNNCC 5 cut(s) 125, 925, 1378, 1974, 2515
NmeAIII GCCGAG 1 cut(s) 181
NmuCI GTSAC 6 cut(s) 646, 659, 724, 958, 1258, 2041
NsbI TGCGCA 1 cut(s) 3210
NsiI ATGCAT 1 cut(s) 1183
NspI RCATGY 3 cut(s) 1332, 2505, 2869
OliI CACNNNNGTG 2 cut(s) 2219, 2531
PaeI GCATGC 1 cut(s) 2505
PagI TCATGA 2 cut(s) 334, 2886
PauI GCGCGC 1 cut(s) 151
PcsI WCGNNNNNNNCGW 3 cut(s) 2342, 3024, 3193
PctI GAATGC 3 cut(s) 591, 1611, 1733
PdmI GAANNNNTTC 3 cut(s) 1199, 2166, 3183
PfeI GAWTC 5 cut(s) 570, 857, 1965, 2730, 3179
PflMI CCANNNNNTGG 1 cut(s) 818
PfoI TCCNGGA 1 cut(s) 183
PinAI ACCGGT 2 cut(s) 2626, 2750
PkrI GCNGC 3 cut(s) 223, 547, 550
Ple19I CGATCG 1 cut(s) 2195
PleI GAGTC 3 cut(s) 912, 1567, 1901
PpsI GAGTC 3 cut(s) 912, 1567, 1901
Ppu21I YACGTR 2 cut(s) 197, 211
PshBI ATTAAT 1 cut(s) 2058
PsiI TTATAA 4 cut(s) 261, 1220, 1647, 2366
Psp124BI GAGCTC 1 cut(s) 2147
Psp6I CCWGG 3 cut(s) 183, 1251, 2808
PspEI GGTNACC 1 cut(s) 1245
PspFI CCCAGC 1 cut(s) 2021
PspGI CCWGG 3 cut(s) 183, 1251, 2808
PspN4I GGNNCC 5 cut(s) 125, 925, 1378, 1974, 2515
PspPI GGNCC 5 cut(s) 70, 87, 978, 2123, 3112
PstI CTGCAG 1 cut(s) 2181
PstNI CAGNNNCTG 1 cut(s) 2264
PsuI RGATCY 1 cut(s) 1972
PteI GCGCGC 1 cut(s) 151
PvuI CGATCG 1 cut(s) 2195
PvuII CAGCTG 1 cut(s) 815
RsaI GTAC 6 cut(s) 23, 213, 513, 1488, 2448, 3253
RsaNI GTAC 6 cut(s) 22, 212, 512, 1487, 2447, 3252
RseI CAYNNNNRTG 7 cut(s) 536, 2219, 2531, 2891, 3084, 3259, 3306
SacI GAGCTC 1 cut(s) 2147
SalI GTCGAC 1 cut(s) 851
SatI GCNGC 3 cut(s) 222, 546, 549
Sau96I GGNCC 5 cut(s) 70, 87, 978, 2123, 3112
ScaI AGTACT 1 cut(s) 23
SchI GAGTC 3 cut(s) 913, 1567, 1901
ScrFI CCNGG 7 cut(s) 185, 1253, 1986, 2656, 2810, 3073, 3074
SduI GDGCHC 2 cut(s) 2147, 2598
SfcI CTRYAG 5 cut(s) 558, 1037, 2115, 2177, 2589
SinI GGWCC 2 cut(s) 87, 978
SmaI CCCGGG 1 cut(s) 3074
SmiMI CAYNNNNRTG 7 cut(s) 536, 2219, 2531, 2891, 3084, 3259, 3306
SmlI CTYRAG 1 cut(s) 3032
SmoI CTYRAG 1 cut(s) 3032
SnaBI TACGTA 1 cut(s) 211
SphI GCATGC 1 cut(s) 2505
SspI AATATT 1 cut(s) 2056
SspMI CTAG 5 cut(s) 635, 729, 1700, 1914, 2015
SstI GAGCTC 1 cut(s) 2147
StyD4I CCNGG 7 cut(s) 183, 1251, 1984, 2654, 2808, 3071, 3072
StyI CCWWGG 1 cut(s) 2217
TaiI ACGT 5 cut(s) 45, 199, 213, 1464, 1949
TaqI TCGA 8 cut(s) 39, 45, 328, 852, 1299, 2191, 3134, 3222
TaqII GACCGA 1 cut(s) 995
TatI WGTACW 4 cut(s) 21, 1486, 2446, 3251
TfiI GAWTC 5 cut(s) 570, 857, 1965, 2730, 3179
TscAI CASTG 8 cut(s) 664, 1263, 1642, 2050, 2298, 2866, 2970, 3266
TseFI GTSAC 6 cut(s) 646, 659, 724, 958, 1258, 2041
TseI GCWGC 3 cut(s) 221, 545, 548
Tsp45I GTSAC 6 cut(s) 646, 659, 724, 958, 1258, 2041
TspGWI ACGGA 5 cut(s) 88, 750, 2351, 2758, 3097
TspMI CCCGGG 1 cut(s) 3072
TspRI CASTG 8 cut(s) 664, 1263, 1642, 2050, 2298, 2866, 2970, 3266
Van91I CCANNNNNTGG 1 cut(s) 818
VpaK11BI GGWCC 2 cut(s) 87, 978
VspI ATTAAT 1 cut(s) 2058
XagI CCTNNNNNAGG 2 cut(s) 480, 1716
XapI RAATTY 5 cut(s) 1598, 2720, 2764, 2933, 3168
XbaI TCTAGA 1 cut(s) 2014
XceI RCATGY 3 cut(s) 1332, 2505, 2869
XmaI CCCGGG 1 cut(s) 3072
XmiI GTMKAC 2 cut(s) 852, 1902
XmnI GAANNNNTTC 3 cut(s) 1199, 2166, 3183
XspI CTAG 5 cut(s) 635, 729, 1700, 1914, 2015
ZraI GACGTC 1 cut(s) 43
ZrmI AGTACT 1 cut(s) 23
Zsp2I ATGCAT 1 cut(s) 1183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.